AlfalfaGEDB | Msa1179260

M. sativa cultivar zhongmu-4 / Msa1179260

KEGG: K09422
GO: GO:0000228 GO:0000723 GO:0000781 GO:0000784 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003691 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009751 GO:0009753 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010468 GO:0010556 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031627 GO:0031974 GO:0031981 GO:0032200 GO:0034641 GO:0042162 GO:0042221 GO:0042493 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0046677 GO:0046686 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098687 GO:0140110 GO:1901360 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1179260	XP_003627170.1	82.623	305	34	3	1	305	1	286	2.63e-170	484

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1179260	sp\|F4IEY4\|TRB5_ARATH	42.395	309	146	7	1	302	1	284	5.63e-64	206

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1179260	G7LI96	82.623	305	34	3	1	305	1	286	1.26e-170	484

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TFs/TRs:

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Gene ID	Type	Classification
Msa1179260	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1179260	MtrunA17_Chr8g0342871	82.623	305	34	3	1	305	1	286	2.42e-174	484
Msa1179260	MtrunA17_Chr2g0329921	47.937	315	140	8	1	304	1	302	1.49e-82	251
Msa1179260	MtrunA17_Chr8g0384671	38.249	217	114	5	1	216	1	198	3.58e-35	129
Msa1179260	MtrunA17_Chr2g0293381	37.900	219	118	5	1	219	1	201	3.46e-34	126
Msa1179260	MtrunA17_Chr4g0054131	39.806	206	107	6	1	197	1	198	1.15e-33	125
Msa1179260	MtrunA17_Chr5g0401061	38.191	199	105	4	1	197	1	183	1.70e-33	125
Msa1179260	MtrunA17_Chr1g0166681	55.556	63	28	0	1	63	1	63	1.71e-18	82.0
Msa1179260	MtrunA17_Chr8g0383821	60.345	58	23	0	1	58	1	58	6.44e-18	79.0
Msa1179260	MtrunA17_Chr7g0260771	43.939	66	36	1	133	197	63	128	7.12e-11	62.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1179260	AT1G72740.2	43.234	303	146	6	1	302	1	278	2.61e-67	212
Msa1179260	AT1G72740.1	42.395	309	146	7	1	302	1	284	5.72e-65	206
Msa1179260	AT1G17520.4	43.137	306	151	5	1	302	1	287	1.62e-64	205
Msa1179260	AT1G17520.3	42.997	307	151	6	1	302	1	288	8.65e-63	201
Msa1179260	AT1G17520.1	42.308	312	151	6	1	302	1	293	5.04e-62	199
Msa1179260	AT1G17520.2	42.173	313	151	7	1	302	1	294	3.62e-60	194
Msa1179260	AT1G49950.3	34.906	212	94	5	1	197	1	183	2.77e-29	114
Msa1179260	AT1G49950.1	34.906	212	94	5	1	197	1	183	2.77e-29	114
Msa1179260	AT1G49950.2	34.906	212	94	5	1	197	1	183	2.77e-29	114
Msa1179260	AT3G49850.1	34.158	202	115	3	1	199	1	187	9.00e-28	109

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Find 69 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTGATCAGGCCGAAA+CGG	0.507533	8_1:-12584823	Msa1179260:CDS
AAAATTTGTAGTCCAGTAAA+TGG	0.305217	8_1:-12584417	Msa1179260:three_prime_UTR
AAAGACATGGCCCTGGTAAA+TGG	0.273347	8_1:-12588125	Msa1179260:CDS
AACCTCAATGAAACTAGCAA+TGG	0.624133	8_1:+12586215	None:intergenic
AACTGAAAACAAATCATATC+TGG	0.352692	8_1:-12584717	Msa1179260:CDS
AACTGAGGCAGTCAAGGCGA+CGG	0.589786	8_1:-12584693	Msa1179260:CDS
AAGGACCGAACTGAGGGTCA+AGG	0.625130	8_1:+12588091	None:intergenic
AAGTTGTCAACACCATTTAC+TGG	0.336743	8_1:+12584405	None:intergenic
ACATCTTGGTCAATTTGAAG+AGG	0.594971	8_1:+12587732	None:intergenic
ACATTGTTCATAAAGTTGTT+CGG	0.449895	8_1:+12584622	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAACATTACTCAAGTTCCT+AGG	-	chr8_1:12584620-12584639	Msa1179260:intron	30.0%
TTAACAACAACATCAGAAGA+AGG	+	chr8_1:12584748-12584767	None:intergenic	30.0%
TTGTTAAAAACAACACACCT+AGG	-	chr8_1:12584824-12584843	Msa1179260:CDS	30.0%
TCTTCTAAACAATTCGTCAT+TGG	+	chr8_1:12584912-12584931	None:intergenic	30.0%
GTCAAATTGTTACAGAATAG+TGG	-	chr8_1:12585063-12585082	Msa1179260:intron	30.0%
ATTGTTACAGAATAGTGGTT+TGG	-	chr8_1:12585068-12585087	Msa1179260:intron	30.0%
AAATAAACACACGCACTAAA+GGG	+	chr8_1:12585148-12585167	None:intergenic	30.0%
GGTTTGTAAATTGATCCTAT+AGG	-	chr8_1:12586022-12586041	Msa1179260:intron	30.0%
CACAGACACTAAAAAATACA+AGG	+	chr8_1:12586911-12586930	None:intergenic	30.0%
TCAAGATCTCTTGGTTAATT+TGG	-	chr8_1:12587012-12587031	Msa1179260:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	12584274	12588282	12584274	ID=Msa1179260;Name=Msa1179260
chr8_1	mRNA	12584274	12588282	12584274	ID=Msa1179260-mRNA-1;Parent=Msa1179260;Name=Msa1179260-mRNA-1;_AED=0.07;_eAED=0.07;_QI=74\|1\|1\|1\|1\|1\|6\|195\|314
chr8_1	exon	12584274	12584527	12584274	ID=Msa1179260-mRNA-1:exon:4502;Parent=Msa1179260-mRNA-1
chr8_1	exon	12584624	12584939	12584624	ID=Msa1179260-mRNA-1:exon:4501;Parent=Msa1179260-mRNA-1
chr8_1	exon	12585851	12585934	12585851	ID=Msa1179260-mRNA-1:exon:4500;Parent=Msa1179260-mRNA-1
chr8_1	exon	12586218	12586302	12586218	ID=Msa1179260-mRNA-1:exon:4499;Parent=Msa1179260-mRNA-1
chr8_1	exon	12587711	12587961	12587711	ID=Msa1179260-mRNA-1:exon:4498;Parent=Msa1179260-mRNA-1
chr8_1	exon	12588059	12588282	12588059	ID=Msa1179260-mRNA-1:exon:4497;Parent=Msa1179260-mRNA-1
chr8_1	five_prime_UTR	12588209	12588282	12588209	ID=Msa1179260-mRNA-1:five_prime_utr;Parent=Msa1179260-mRNA-1
chr8_1	CDS	12588059	12588208	12588059	ID=Msa1179260-mRNA-1:cds;Parent=Msa1179260-mRNA-1
chr8_1	CDS	12587711	12587961	12587711	ID=Msa1179260-mRNA-1:cds;Parent=Msa1179260-mRNA-1
chr8_1	CDS	12586218	12586302	12586218	ID=Msa1179260-mRNA-1:cds;Parent=Msa1179260-mRNA-1
chr8_1	CDS	12585851	12585934	12585851	ID=Msa1179260-mRNA-1:cds;Parent=Msa1179260-mRNA-1
chr8_1	CDS	12584624	12584939	12584624	ID=Msa1179260-mRNA-1:cds;Parent=Msa1179260-mRNA-1
chr8_1	CDS	12584469	12584527	12584469	ID=Msa1179260-mRNA-1:cds;Parent=Msa1179260-mRNA-1
chr8_1	three_prime_UTR	12584274	12584468	12584274	ID=Msa1179260-mRNA-1:three_prime_utr;Parent=Msa1179260-mRNA-1

Gene Sequence

>Msa1179260

ATGGGAAATCAAAAGCTGAAATGGACGAATGAAGAAGAAGACGCTCTTATCGCTGGAATCGAAAGACATGGCCCTGGTAAATGGAAAGCCATTCTCCTTGACCCTCAGTTCGGTCCTTTACTCACTTCTCGTTCCAACATCGACCTCAAGGATAAATGGAGGAATATGAATGTGAATAACATTACTCAAGTTCCTAGGTTTCCCAAATGCAAACCTCCTGCTGCTGCTGCTTCTTCTCCTGTTCCTGCTGCTACTCCAGTTTCTTCTTCCAGTGCTGCTGTTAATCAAAATGTTGCTCTTCCTTCTTCTGATGTTGTTGTTAACAATGTTACTCCTCCTCTTCAAATTGTTCCTCCACCTCTTCAAATTGACCAAGATGTTGTTAAAAACAACACACCTAGGTATGATGTCATGATTTATGAAGCTCTGACTACATTAAAGGATACTAATGGATCCGACGTAAATGCCATTGCTAGTTTCATTGAGCAAAAGCATCCGGTTCCTCAAAATTTCAGAAAGTCACTGACTGCAAGGTTGAGGATGCTTGTGAGTCAAGGCAAGCTTGAAAAGGAACAAAATTGTTTCAAGATAAAAGAAGCCTTGTTGGTGAAAAAATCACCTTCACCAAAACAAAAGGAGGCCAAGCCATCACCTTCAGCAAAACAAAAGGTGATCAGGCCGAAACGGCAATCATCAGTCTCTGATGATTCATCAGTCTCTGATGATATGCTAAAGGAAGCAGCTGAAACTGCAGCATACATAATTGCTGAAACTGAAAACAAATCATATCTGGCAACTGAGGCAGTCAAGGCGACGGAAAAATACTCACGCATGGCTGAAGAAAATGATGCGATGTTGTTGCTAGCCGAACAACTTTATGAACAATGTTTGCGCGGTGAAACTATCAAATGGGCTCAAGATGGTCAATGCAAGATTGATAAATAG

Protein sequence

>Msa1179260

MGNQKLKWTNEEEDALIAGIERHGPGKWKAILLDPQFGPLLTSRSNIDLKDKWRNMNVNNITQVPRFPKCKPPAAAASSPVPAATPVSSSSAAVNQNVALPSSDVVVNNVTPPLQIVPPPLQIDQDVVKNNTPRYDVMIYEALTTLKDTNGSDVNAIASFIEQKHPVPQNFRKSLTARLRMLVSQGKLEKEQNCFKIKEALLVKKSPSPKQKEAKPSPSAKQKVIRPKRQSSVSDDSSVSDDMLKEAAETAAYIIAETENKSYLATEAVKATEKYSRMAEENDAMLLLAEQLYEQCLRGETIKWAQDGQCKIDK*