Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1185770 | GAU41275.1 | 63.291 | 158 | 52 | 2 | 1 | 152 | 1 | 158 | 1.71e-58 | 192 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1185770 | sp|Q9FJK3|AGL80_ARATH | 47.097 | 155 | 75 | 3 | 1 | 149 | 1 | 154 | 4.11e-33 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1185770 | A0A2Z6NZ76 | 63.291 | 158 | 52 | 2 | 1 | 152 | 1 | 158 | 8.16e-59 | 192 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1185770 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1185770 | MtrunA17_Chr8g0351751 | 68.750 | 160 | 42 | 3 | 1 | 152 | 1 | 160 | 1.41e-57 | 180 |
| Msa1185770 | MtrunA17_Chr3g0107381 | 57.419 | 155 | 59 | 4 | 1 | 149 | 1 | 154 | 6.77e-53 | 167 |
| Msa1185770 | MtrunA17_Chr3g0110131 | 58.710 | 155 | 57 | 3 | 1 | 149 | 1 | 154 | 3.26e-49 | 158 |
| Msa1185770 | MtrunA17_Chr4g0014031 | 55.063 | 158 | 65 | 2 | 1 | 152 | 1 | 158 | 2.29e-46 | 150 |
| Msa1185770 | MtrunA17_Chr4g0013041 | 52.597 | 154 | 65 | 3 | 1 | 148 | 1 | 152 | 2.71e-45 | 147 |
| Msa1185770 | MtrunA17_Chr4g0014321 | 50.000 | 158 | 73 | 2 | 1 | 152 | 1 | 158 | 6.93e-43 | 142 |
| Msa1185770 | MtrunA17_Chr4g0014131 | 54.717 | 159 | 66 | 2 | 1 | 153 | 1 | 159 | 6.45e-42 | 139 |
| Msa1185770 | MtrunA17_Chr4g0014121 | 52.830 | 159 | 69 | 2 | 1 | 153 | 29 | 187 | 3.09e-40 | 134 |
| Msa1185770 | MtrunA17_Chr2g0296831 | 59.350 | 123 | 42 | 3 | 1 | 116 | 1 | 122 | 7.66e-37 | 123 |
| Msa1185770 | MtrunA17_Chr3g0107331 | 43.919 | 148 | 76 | 3 | 3 | 144 | 2 | 148 | 3.28e-34 | 117 |
| Msa1185770 | MtrunA17_Chr3g0107351 | 41.892 | 148 | 79 | 3 | 3 | 144 | 2 | 148 | 4.00e-32 | 112 |
| Msa1185770 | MtrunA17_Chr3g0107361 | 42.568 | 148 | 78 | 3 | 3 | 144 | 2 | 148 | 4.15e-31 | 110 |
| Msa1185770 | MtrunA17_Chr3g0107341 | 41.892 | 148 | 79 | 3 | 3 | 144 | 2 | 148 | 2.08e-30 | 108 |
| Msa1185770 | MtrunA17_Chr3g0107461 | 37.500 | 152 | 89 | 3 | 1 | 146 | 1 | 152 | 1.30e-29 | 106 |
| Msa1185770 | MtrunA17_Chr5g0420351 | 39.744 | 156 | 86 | 4 | 1 | 150 | 1 | 154 | 1.41e-28 | 103 |
| Msa1185770 | MtrunA17_Chr2g0283941 | 37.736 | 159 | 93 | 3 | 1 | 153 | 1 | 159 | 4.00e-26 | 99.4 |
| Msa1185770 | MtrunA17_Chr4g0031421 | 37.705 | 122 | 71 | 1 | 30 | 146 | 3 | 124 | 4.89e-23 | 89.0 |
| Msa1185770 | MtrunA17_Chr5g0432691 | 33.775 | 151 | 93 | 3 | 1 | 145 | 1 | 150 | 2.74e-22 | 87.4 |
| Msa1185770 | MtrunA17_Chr7g0218151 | 39.073 | 151 | 84 | 4 | 1 | 145 | 1 | 149 | 1.68e-21 | 85.1 |
| Msa1185770 | MtrunA17_Chr3g0105791 | 38.017 | 121 | 70 | 2 | 30 | 145 | 3 | 123 | 2.45e-21 | 84.3 |
| Msa1185770 | MtrunA17_Chr2g0296851 | 57.647 | 85 | 36 | 0 | 68 | 152 | 1 | 85 | 3.86e-21 | 82.4 |
| Msa1185770 | MtrunA17_Chr1g0188681 | 33.775 | 151 | 92 | 4 | 1 | 145 | 1 | 149 | 2.68e-20 | 82.0 |
| Msa1185770 | MtrunA17_Chr1g0193311 | 35.542 | 166 | 79 | 5 | 1 | 150 | 1 | 154 | 2.79e-20 | 82.0 |
| Msa1185770 | MtrunA17_Chr1g0197321 | 31.646 | 158 | 101 | 3 | 1 | 152 | 1 | 157 | 3.59e-19 | 79.3 |
| Msa1185770 | MtrunA17_Chr1g0188661 | 34.437 | 151 | 91 | 4 | 1 | 145 | 1 | 149 | 8.92e-19 | 78.2 |
| Msa1185770 | MtrunA17_Chr4g0012761 | 35.099 | 151 | 90 | 4 | 1 | 145 | 1 | 149 | 1.06e-18 | 78.2 |
| Msa1185770 | MtrunA17_Chr1g0197461 | 33.333 | 153 | 83 | 5 | 3 | 149 | 2 | 141 | 1.85e-18 | 77.4 |
| Msa1185770 | MtrunA17_Chr1g0197261 | 31.788 | 151 | 95 | 4 | 1 | 145 | 1 | 149 | 2.22e-18 | 77.0 |
| Msa1185770 | MtrunA17_Chr2g0296861 | 36.538 | 156 | 92 | 4 | 1 | 150 | 1 | 155 | 4.18e-18 | 76.6 |
| Msa1185770 | MtrunA17_Chr1g0188651 | 35.762 | 151 | 87 | 4 | 1 | 145 | 1 | 147 | 6.92e-15 | 68.2 |
| Msa1185770 | MtrunA17_Chr3g0106421 | 30.952 | 126 | 82 | 2 | 30 | 150 | 3 | 128 | 1.55e-12 | 62.8 |
| Msa1185770 | MtrunA17_Chr3g0106501 | 28.906 | 128 | 86 | 1 | 31 | 153 | 4 | 131 | 2.95e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1185770 | AT5G48670.1 | 47.097 | 155 | 75 | 3 | 1 | 149 | 1 | 154 | 4.18e-34 | 122 |
| Msa1185770 | AT5G26630.1 | 38.710 | 155 | 88 | 3 | 1 | 149 | 1 | 154 | 6.83e-23 | 90.9 |
| Msa1185770 | AT1G65330.1 | 41.176 | 119 | 64 | 3 | 3 | 115 | 2 | 120 | 1.29e-22 | 91.3 |
| Msa1185770 | AT5G27810.1 | 42.308 | 104 | 49 | 3 | 30 | 128 | 3 | 100 | 2.38e-21 | 84.0 |
| Msa1185770 | AT1G65300.1 | 35.948 | 153 | 91 | 4 | 3 | 149 | 2 | 153 | 1.30e-20 | 85.9 |
| Msa1185770 | AT5G27960.1 | 38.519 | 135 | 76 | 4 | 4 | 132 | 2 | 135 | 1.91e-20 | 86.3 |
| Msa1185770 | AT5G26650.1 | 37.037 | 135 | 78 | 4 | 4 | 132 | 2 | 135 | 3.63e-20 | 85.9 |
| Msa1185770 | AT1G31630.1 | 30.667 | 150 | 94 | 4 | 3 | 145 | 2 | 148 | 1.95e-19 | 83.6 |
| Msa1185770 | AT3G05860.2 | 37.600 | 125 | 71 | 3 | 1 | 119 | 1 | 124 | 6.24e-18 | 77.4 |
| Msa1185770 | AT3G05860.3 | 37.600 | 125 | 71 | 3 | 1 | 119 | 1 | 124 | 8.27e-18 | 78.2 |
| Msa1185770 | AT3G05860.1 | 37.600 | 125 | 71 | 3 | 1 | 119 | 1 | 124 | 1.02e-17 | 77.8 |
| Msa1185770 | AT5G26580.1 | 33.813 | 139 | 69 | 4 | 1 | 132 | 1 | 123 | 1.53e-16 | 75.9 |
| Msa1185770 | AT1G31640.1 | 30.882 | 136 | 86 | 4 | 3 | 132 | 2 | 135 | 3.35e-14 | 69.3 |
| Msa1185770 | AT2G28700.1 | 29.333 | 150 | 99 | 3 | 1 | 144 | 1 | 149 | 1.81e-12 | 64.3 |
Find 36 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTGCTTTGTTCTATTTC+AGG | 0.134324 | 8_1:+23221218 | None:intergenic |
| TAAGACATTGAAATATTAGA+AGG | 0.315541 | 8_1:+23221099 | None:intergenic |
| GAGTAGTCATGATTTGAATT+TGG | 0.321998 | 8_1:+23220944 | None:intergenic |
| GAAGGACATCGATAGAATAT+TGG | 0.362429 | 8_1:-23221000 | Msa1185770:CDS |
| AATGATCATGCTTGGTTGAT+TGG | 0.365792 | 8_1:-23221032 | Msa1185770:CDS |
| TCATTGTCAATGATACTATA+AGG | 0.367700 | 8_1:-23221407 | Msa1185770:CDS |
| AAAAGGTACTCTCGAAATTC+AGG | 0.396888 | 8_1:-23221247 | Msa1185770:CDS |
| TGAATTTCGAGAGTACCTTT+TGG | 0.396952 | 8_1:+23221249 | None:intergenic |
| GATGAACTAAACACTCTTTG+TGG | 0.400357 | 8_1:-23221342 | Msa1185770:CDS |
| AGAAGCAAAAGAATGACAAT+AGG | 0.400807 | 8_1:-23221136 | Msa1185770:CDS |
| ATCTAATGTTGCGGTTCATA+AGG | 0.404391 | 8_1:+23221291 | None:intergenic |
| TTAGATGTGGCCATCCCCTT+GGG | 0.405563 | 8_1:-23221274 | Msa1185770:CDS |
| GGAAAGTAACATATAAGAAA+AGG | 0.424806 | 8_1:-23221386 | Msa1185770:CDS |
| TATAATAATGGTCAAATTGA+AGG | 0.460220 | 8_1:-23220967 | Msa1185770:CDS |
| GAAGCAAAAGAATGACAATA+GGG | 0.466782 | 8_1:-23221135 | Msa1185770:CDS |
| AACATATAAGAAAAGGAAGA+AGG | 0.481425 | 8_1:-23221379 | Msa1185770:CDS |
| ATCGATAGAATATTGGAAGC+AGG | 0.484523 | 8_1:-23220993 | Msa1185770:CDS |
| ATTAGATGTGGCCATCCCCT+TGG | 0.484632 | 8_1:-23221275 | Msa1185770:CDS |
| GTTGATTGGTCACAATTTGA+AGG | 0.487875 | 8_1:-23221018 | Msa1185770:CDS |
| CCATCCCCTTGGGGTCCAAA+AGG | 0.499637 | 8_1:-23221264 | Msa1185770:CDS |
| GGAAGCAGGGGATATAATAA+TGG | 0.505439 | 8_1:-23220979 | Msa1185770:CDS |
| ATATTTCAATGTCTTAATGC+AGG | 0.505576 | 8_1:-23221092 | Msa1185770:CDS |
| CATGAAACAAAGGGTGGTGA+AGG | 0.522218 | 8_1:-23221174 | Msa1185770:CDS |
| TATAGTATCATTGACAATGA+AGG | 0.523085 | 8_1:+23221410 | None:intergenic |
| TGGGCACAACAATATGTCAA+TGG | 0.545200 | 8_1:-23221063 | Msa1185770:CDS |
| AGAGACTTTCATGAAACAAA+GGG | 0.552731 | 8_1:-23221183 | Msa1185770:CDS |
| AAGAGACTTTCATGAAACAA+AGG | 0.557993 | 8_1:-23221184 | Msa1185770:CDS |
| CGATAGAATATTGGAAGCAG+GGG | 0.564591 | 8_1:-23220991 | Msa1185770:CDS |
| TCGATAGAATATTGGAAGCA+GGG | 0.568198 | 8_1:-23220992 | Msa1185770:CDS |
| ACATATAAGAAAAGGAAGAA+GGG | 0.584169 | 8_1:-23221378 | Msa1185770:CDS |
| AAGACATTGAAATATTAGAA+GGG | 0.587962 | 8_1:+23221100 | None:intergenic |
| TTGATGTGAATGATCATGCT+TGG | 0.606958 | 8_1:-23221040 | Msa1185770:CDS |
| TAGATGTGGCCATCCCCTTG+GGG | 0.619614 | 8_1:-23221273 | Msa1185770:CDS |
| ATGAACCGCAACATTAGATG+TGG | 0.642488 | 8_1:-23221287 | Msa1185770:CDS |
| GATGGCCACATCTAATGTTG+CGG | 0.647650 | 8_1:+23221282 | None:intergenic |
| GACTTTCATGAAACAAAGGG+TGG | 0.665794 | 8_1:-23221180 | Msa1185770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGACATTGAAATATTAGAA+GGG | + | chr8_1:23221308-23221327 | None:intergenic | 20.0% |
| !! | TAAGACATTGAAATATTAGA+AGG | + | chr8_1:23221309-23221328 | None:intergenic | 20.0% |
| !! | TATAATAATGGTCAAATTGA+AGG | - | chr8_1:23221438-23221457 | Msa1185770:CDS | 20.0% |
| ! | TATAGTATCATTGACAATGA+AGG | + | chr8_1:23220998-23221017 | None:intergenic | 25.0% |
| ! | TCATTGTCAATGATACTATA+AGG | - | chr8_1:23220998-23221017 | Msa1185770:CDS | 25.0% |
| ! | GGAAAGTAACATATAAGAAA+AGG | - | chr8_1:23221019-23221038 | Msa1185770:CDS | 25.0% |
| ! | AACATATAAGAAAAGGAAGA+AGG | - | chr8_1:23221026-23221045 | Msa1185770:CDS | 25.0% |
| ! | ACATATAAGAAAAGGAAGAA+GGG | - | chr8_1:23221027-23221046 | Msa1185770:CDS | 25.0% |
| ! | ATATTTCAATGTCTTAATGC+AGG | - | chr8_1:23221313-23221332 | Msa1185770:intron | 25.0% |
| !!! | TTTCACCTTTTTTCTAGTCA+TGG | + | chr8_1:23220971-23220990 | None:intergenic | 30.0% |
| GAAGTTTGTGCTATAGTTTA+TGG | - | chr8_1:23221090-23221109 | Msa1185770:CDS | 30.0% | |
| ! | TTTCTGCTTTGTTCTATTTC+AGG | + | chr8_1:23221190-23221209 | None:intergenic | 30.0% |
| AAGAGACTTTCATGAAACAA+AGG | - | chr8_1:23221221-23221240 | Msa1185770:CDS | 30.0% | |
| AGAGACTTTCATGAAACAAA+GGG | - | chr8_1:23221222-23221241 | Msa1185770:CDS | 30.0% | |
| AGAAGCAAAAGAATGACAAT+AGG | - | chr8_1:23221269-23221288 | Msa1185770:CDS | 30.0% | |
| GAAGCAAAAGAATGACAATA+GGG | - | chr8_1:23221270-23221289 | Msa1185770:CDS | 30.0% | |
| TGTCTTAATGCAGGAAAAAT+TGG | - | chr8_1:23221322-23221341 | Msa1185770:intron | 30.0% | |
| GTCTTAATGCAGGAAAAATT+GGG | - | chr8_1:23221323-23221342 | Msa1185770:intron | 30.0% | |
| CACAACCATGACTAGAAAAA+AGG | - | chr8_1:23220963-23220982 | Msa1185770:CDS | 35.0% | |
| GATGAACTAAACACTCTTTG+TGG | - | chr8_1:23221063-23221082 | Msa1185770:CDS | 35.0% | |
| ATCTAATGTTGCGGTTCATA+AGG | + | chr8_1:23221117-23221136 | None:intergenic | 35.0% | |
| !!! | TGAATTTCGAGAGTACCTTT+TGG | + | chr8_1:23221159-23221178 | None:intergenic | 35.0% |
| !! | AAAAGGTACTCTCGAAATTC+AGG | - | chr8_1:23221158-23221177 | Msa1185770:CDS | 35.0% |
| TTGATGTGAATGATCATGCT+TGG | - | chr8_1:23221365-23221384 | Msa1185770:CDS | 35.0% | |
| AATGATCATGCTTGGTTGAT+TGG | - | chr8_1:23221373-23221392 | Msa1185770:CDS | 35.0% | |
| ! | GTTGATTGGTCACAATTTGA+AGG | - | chr8_1:23221387-23221406 | Msa1185770:CDS | 35.0% |
| GAAGGACATCGATAGAATAT+TGG | - | chr8_1:23221405-23221424 | Msa1185770:CDS | 35.0% | |
| ATCGATAGAATATTGGAAGC+AGG | - | chr8_1:23221412-23221431 | Msa1185770:CDS | 35.0% | |
| TCGATAGAATATTGGAAGCA+GGG | - | chr8_1:23221413-23221432 | Msa1185770:CDS | 35.0% | |
| ATGAACCGCAACATTAGATG+TGG | - | chr8_1:23221118-23221137 | Msa1185770:CDS | 40.0% | |
| GACTTTCATGAAACAAAGGG+TGG | - | chr8_1:23221225-23221244 | Msa1185770:CDS | 40.0% | |
| TGGGCACAACAATATGTCAA+TGG | - | chr8_1:23221342-23221361 | Msa1185770:CDS | 40.0% | |
| CGATAGAATATTGGAAGCAG+GGG | - | chr8_1:23221414-23221433 | Msa1185770:CDS | 40.0% | |
| GGAAGCAGGGGATATAATAA+TGG | - | chr8_1:23221426-23221445 | Msa1185770:CDS | 40.0% | |
| GATGGCCACATCTAATGTTG+CGG | + | chr8_1:23221126-23221145 | None:intergenic | 45.0% | |
| CATGAAACAAAGGGTGGTGA+AGG | - | chr8_1:23221231-23221250 | Msa1185770:CDS | 45.0% | |
| ATTAGATGTGGCCATCCCCT+TGG | - | chr8_1:23221130-23221149 | Msa1185770:CDS | 50.0% | |
| TTAGATGTGGCCATCCCCTT+GGG | - | chr8_1:23221131-23221150 | Msa1185770:CDS | 50.0% | |
| ! | AGTACCTTTTGGACCCCAAG+GGG | + | chr8_1:23221148-23221167 | None:intergenic | 50.0% |
| ! | GAGTACCTTTTGGACCCCAA+GGG | + | chr8_1:23221149-23221168 | None:intergenic | 50.0% |
| !! | AGAGTACCTTTTGGACCCCA+AGG | + | chr8_1:23221150-23221169 | None:intergenic | 50.0% |
| TAGATGTGGCCATCCCCTTG+GGG | - | chr8_1:23221132-23221151 | Msa1185770:CDS | 55.0% | |
| ! | CCTTTTGGACCCCAAGGGGA+TGG | + | chr8_1:23221144-23221163 | None:intergenic | 60.0% |
| CCATCCCCTTGGGGTCCAAA+AGG | - | chr8_1:23221141-23221160 | Msa1185770:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 23220953 | 23221474 | 23220953 | ID=Msa1185770;Name=Msa1185770 |
| chr8_1 | mRNA | 23220953 | 23221474 | 23220953 | ID=Msa1185770-mRNA-1;Parent=Msa1185770;Name=Msa1185770-mRNA-1;_AED=0.08;_eAED=0.17;_QI=17|0|0|1|0|0|2|0|164 |
| chr8_1 | exon | 23221335 | 23221474 | 23221335 | ID=Msa1185770-mRNA-1:exon:8558;Parent=Msa1185770-mRNA-1 |
| chr8_1 | exon | 23220953 | 23221321 | 23220953 | ID=Msa1185770-mRNA-1:exon:8557;Parent=Msa1185770-mRNA-1 |
| chr8_1 | five_prime_UTR | 23221458 | 23221474 | 23221458 | ID=Msa1185770-mRNA-1:five_prime_utr;Parent=Msa1185770-mRNA-1 |
| chr8_1 | CDS | 23221335 | 23221457 | 23221335 | ID=Msa1185770-mRNA-1:cds;Parent=Msa1185770-mRNA-1 |
| chr8_1 | CDS | 23220953 | 23221321 | 23220953 | ID=Msa1185770-mRNA-1:cds;Parent=Msa1185770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
