AlfalfaGEDB | Msa1187640

M. sativa cultivar zhongmu-4 / Msa1187640

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1187640	XP_003628084.3	88.922	334	12	3	1	309	222	555	0.0	612

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1187640	sp\|Q1PEM5\|PERK3_ARATH	40.184	326	146	8	50	326	144	469	1.34e-63	214

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1187640	MtrunA17_Chr8g0354651	89.399	283	11	2	1	264	37	319	0.0	520
Msa1187640	MtrunA17_Chr3g0080901	66.997	303	74	4	42	318	94	396	1.12e-139	406
Msa1187640	MtrunA17_Chr3g0080881	61.290	310	90	7	30	309	25	334	1.35e-122	358
Msa1187640	MtrunA17_Chr3g0093601	45.066	304	140	5	34	310	64	367	6.92e-81	251
Msa1187640	MtrunA17_Chr2g0330471	47.039	304	127	8	47	316	377	680	1.64e-78	254
Msa1187640	MtrunA17_Chr8g0341881	47.368	304	126	8	47	316	375	678	2.27e-78	255
Msa1187640	MtrunA17_Chr3g0093591	44.918	305	138	7	39	314	95	398	4.20e-78	246
Msa1187640	MtrunA17_Chr8g0389251	45.724	304	131	9	47	316	374	677	1.33e-75	246
Msa1187640	MtrunA17_Chr3g0093611	41.391	302	142	5	46	312	35	336	6.59e-75	235
Msa1187640	MtrunA17_Chr3g0125631	43.060	281	123	7	64	309	61	339	1.24e-63	206

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1187640	AT1G55200.3	46.497	314	132	9	47	325	349	661	1.75e-78	254
Msa1187640	AT1G55200.4	46.497	314	132	9	47	325	349	661	1.75e-78	254
Msa1187640	AT1G55200.1	46.497	314	132	9	47	325	349	661	1.75e-78	254
Msa1187640	AT1G55200.2	46.497	314	132	9	47	325	349	661	1.75e-78	254
Msa1187640	AT5G56790.1	46.129	310	133	8	47	322	360	669	4.01e-78	253
Msa1187640	AT3G13690.2	45.902	305	129	9	47	316	381	684	8.53e-74	243
Msa1187640	AT3G13690.1	45.902	305	129	9	47	316	381	684	8.53e-74	243
Msa1187640	AT3G13690.3	45.902	305	129	9	47	316	381	684	8.53e-74	243
Msa1187640	AT3G24540.1	42.475	299	136	7	64	326	171	469	4.44e-65	214
Msa1187640	AT1G23540.1	40.060	332	158	10	64	357	359	687	1.14e-62	213

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Find 58 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATACACATCTGACT+TGG	0.618312	8_1:+27509119	None:intergenic
AAAGATCTAGATATGGCAAG+AGG	0.653248	8_1:-27510307	None:intergenic
AAATCCTTAATGATTCAGTG+AGG	0.740201	8_1:-27508028	Msa1187640:CDS
AAGTCAGATGTGTATTCTTT+TGG	0.289391	8_1:-27509117	Msa1187640:CDS
ACACAAAAGTCTTGTGGGAT+GGG	0.501905	8_1:-27509040	Msa1187640:intron
ACAGATGTTGTTGGAACATT+AGG	0.251263	8_1:-27509183	Msa1187640:CDS
AGCCAGGATCAAAGTGGCAA+TGG	0.512784	8_1:-27509468	Msa1187640:CDS
AGGACCACAGACAAAAGGAT+TGG	0.547529	8_1:-27509063	Msa1187640:CDS
ATCCAGATTTAATTGATGAA+AGG	0.534741	8_1:-27508657	Msa1187640:CDS
ATCCTTTCATCAATTAAATC+TGG	0.319310	8_1:+27508655	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAAGCAATACATGAAAATG+TGG	-	chr8_1:27508312-27508331	Msa1187640:intron	30.0%
GATATTTGTGGAGATGATTT+AGG	-	chr8_1:27508572-27508591	Msa1187640:CDS	30.0%
CTTCATAATCATGTGTAACA+AGG	+	chr8_1:27508864-27508883	None:intergenic	30.0%
TACACATGATTATGAAGCAA+TGG	-	chr8_1:27508867-27508886	Msa1187640:intron	30.0%
AAAAGAATACACATCTGACT+TGG	+	chr8_1:27509120-27509139	None:intergenic	30.0%
GATATACTTACAGTATCCAT+TGG	+	chr8_1:27509692-27509711	None:intergenic	30.0%
AGAATTCGACGTAGTTATTA+TGG	+	chr8_1:27509818-27509837	None:intergenic	30.0%
AAATCCTTAATGATTCAGTG+AGG	-	chr8_1:27510208-27510227	Msa1187640:CDS	30.0%
ATGCAATATTTCCATTACAC+AGG	-	chr8_1:27510236-27510255	Msa1187640:CDS	30.0%
CAAAGCAATACATGAAAATG+TGG	-	chr8_1:27508312-27508331	Msa1187640:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	27507941	27510317	27507941	ID=Msa1187640;Name=Msa1187640
chr8_1	mRNA	27507941	27510317	27507941	ID=Msa1187640-mRNA-1;Parent=Msa1187640;Name=Msa1187640-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0\|0\|0\|1\|0.8\|0.66\|6\|0\|360
chr8_1	exon	27510095	27510317	27510095	ID=Msa1187640-mRNA-1:exon:9659;Parent=Msa1187640-mRNA-1
chr8_1	exon	27509834	27510067	27509834	ID=Msa1187640-mRNA-1:exon:9658;Parent=Msa1187640-mRNA-1
chr8_1	exon	27509370	27509488	27509370	ID=Msa1187640-mRNA-1:exon:9657;Parent=Msa1187640-mRNA-1
chr8_1	exon	27509041	27509256	27509041	ID=Msa1187640-mRNA-1:exon:9656;Parent=Msa1187640-mRNA-1
chr8_1	exon	27508554	27508689	27508554	ID=Msa1187640-mRNA-1:exon:9655;Parent=Msa1187640-mRNA-1
chr8_1	exon	27507941	27508095	27507941	ID=Msa1187640-mRNA-1:exon:9654;Parent=Msa1187640-mRNA-1
chr8_1	CDS	27510095	27510317	27510095	ID=Msa1187640-mRNA-1:cds;Parent=Msa1187640-mRNA-1
chr8_1	CDS	27509834	27510067	27509834	ID=Msa1187640-mRNA-1:cds;Parent=Msa1187640-mRNA-1
chr8_1	CDS	27509370	27509488	27509370	ID=Msa1187640-mRNA-1:cds;Parent=Msa1187640-mRNA-1
chr8_1	CDS	27509041	27509256	27509041	ID=Msa1187640-mRNA-1:cds;Parent=Msa1187640-mRNA-1
chr8_1	CDS	27508554	27508689	27508554	ID=Msa1187640-mRNA-1:cds;Parent=Msa1187640-mRNA-1
chr8_1	CDS	27507941	27508095	27507941	ID=Msa1187640-mRNA-1:cds;Parent=Msa1187640-mRNA-1

Gene Sequence

>Msa1187640

ATGGCAAGAGGCTATTGCATATTACCAACTCCTGATAATCAAAAGACAAGAGTTCTTGAAATGCATCACAAAGATTCAACATTTAAACATATCAGAAACCATAAAACATATCAGATCGTTGAAAATTTTAAAAATTCTGTGTGCTCAGTGTGTGAGAACAAAAGGCCAAAATTTGAATTGATGAAAGAATTCACATATGGAGAGTTGCATGAAGCAACGCATGAAGGTGGTTTCGGTTCTGTTTATTGGGGAAAGATGCAAGGGCTCAAGATCGCTGTTAAGAAACATAAATTTGCAAGCCTTCAAGGAGAGAAGGAATTCAAATCTGAAGTTAATGCACTCAACAAAGCAATACATGAAAATGTGGTCATGCTGCTAGGATCTTGTTCGGAGGGAAAGAACAGATTTCTTGTATATGAATTTGTTTGCAATGGCTCACTTGATCAACATTTATCGCCCAGGATCAAAGTGGCAATGGGGGCTGCAAAAGGCTTGTTGTATTTGCATGAGAATAACATCATACACAGAGATATGAGACCAAGTAACATCCTTGTTACACATGATTATGAAGCAATGATTGGAGATTTTGGTCTAGCTAGGACTGAACAAATGGACTCAGTGTACTCAACAGATGTTGTTGGAACATTAGGGTATATGGCACCAGAATATGCAGAATCTGGTAAAGTCTCAACCAAGTCAGATGTGTATTCTTTTGGAGTGGTGCTTCTTCAGTTAATCACAGGAATGAGGACCACAGACAAAAGGATTGGACACAAAAGTCTTGTGGGATGGGAAAGGAACTATCCAGATTTAATTGATGAAAGGATGATGGATACCTATGATTGCCATCAGCTTTTTTGGATGATTCGTTTAGCAGAAAAATGTCTCACTAGGGATCCTCAAAAACGATTACCAATGGATACTGTAAATGAGATTGATGATGATGATGATGGAGAGAATGAGATTGATGCCAAAATCCTTAATGATTCAGTGAGGAAAGCATGCAATATTTCCATTACACAGGAAACAATCTTGAGGTTTCCAGAGTTTTTTGATGGGGGAGAAGCTTCAAATGCAGGGTAA

Protein sequence

>Msa1187640

MARGYCILPTPDNQKTRVLEMHHKDSTFKHIRNHKTYQIVENFKNSVCSVCENKRPKFELMKEFTYGELHEATHEGGFGSVYWGKMQGLKIAVKKHKFASLQGEKEFKSEVNALNKAIHENVVMLLGSCSEGKNRFLVYEFVCNGSLDQHLSPRIKVAMGAAKGLLYLHENNIIHRDMRPSNILVTHDYEAMIGDFGLARTEQMDSVYSTDVVGTLGYMAPEYAESGKVSTKSDVYSFGVVLLQLITGMRTTDKRIGHKSLVGWERNYPDLIDERMMDTYDCHQLFWMIRLAEKCLTRDPQKRLPMDTVNEIDDDDDGENEIDAKILNDSVRKACNISITQETILRFPEFFDGGEASNAG*