AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1188190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1188190 MtrunA17_Chr8g0355261 92.840 419 13 3 38 455 37 439 0.0 795
Msa1188190 MtrunA17_Chr6g0453321 36.409 401 169 13 111 454 123 494 3.87e-62 209
Msa1188190 MtrunA17_Chr4g0042481 35.567 388 168 13 113 453 111 463 2.69e-60 204
Msa1188190 MtrunA17_Chr7g0217581 34.978 446 205 18 53 454 178 582 7.99e-60 205
Msa1188190 MtrunA17_Chr3g0092111 35.129 427 199 14 70 453 72 463 2.52e-57 196
Msa1188190 MtrunA17_Chr7g0254161 37.037 378 168 12 109 455 86 424 5.39e-56 191
Msa1188190 MtrunA17_Chr5g0417551 33.152 368 147 16 94 455 97 371 3.66e-42 153
Msa1188190 MtrunA17_Chr6g0460721 34.375 320 147 11 72 350 32 329 9.95e-40 145
Msa1188190 MtrunA17_Chr6g0460731 31.627 332 149 11 93 364 54 367 1.34e-34 132
Msa1188190 MtrunA17_Chr2g0283811 75.000 64 16 0 114 177 37 100 3.43e-32 120
Msa1188190 MtrunA17_Chr5g0417571 64.198 81 29 0 99 179 113 193 2.84e-31 122
Msa1188190 MtrunA17_Chr5g0417601 62.338 77 29 0 101 177 117 193 1.74e-29 118
Msa1188190 MtrunA17_Chr5g0417601 33.158 190 70 9 278 455 237 381 1.08e-13 72.4
Msa1188190 MtrunA17_Chr5g0417561 53.684 95 44 0 87 181 82 176 6.09e-29 117
Msa1188190 MtrunA17_Chr4g0010151 67.797 59 19 0 120 178 78 136 6.27e-25 105
Msa1188190 MtrunA17_Chr3g0127201 59.701 67 27 0 113 179 16 82 7.29e-25 103
Msa1188190 MtrunA17_Chr2g0308011 74.000 50 13 0 123 172 36 85 1.69e-24 100
Msa1188190 MtrunA17_Chr8g0374111 55.714 70 31 0 124 193 29 98 3.79e-24 100
Msa1188190 MtrunA17_Chr4g0061761 71.698 53 15 0 122 174 38 90 4.41e-24 102
Msa1188190 MtrunA17_Chr8g0346551 61.290 62 24 0 119 180 19 80 7.74e-24 100
Msa1188190 MtrunA17_Chr2g0328021 52.809 89 38 2 95 183 37 121 8.65e-24 100
Msa1188190 MtrunA17_Chr4g0031411 66.102 59 20 0 120 178 71 129 1.42e-23 101
Msa1188190 MtrunA17_Chr2g0326001 63.333 60 22 0 119 178 35 94 2.21e-23 99.8
Msa1188190 MtrunA17_Chr8g0374251 72.222 54 15 0 124 177 68 121 2.72e-23 100
Msa1188190 MtrunA17_Chr8g0341671 69.231 52 16 0 124 175 20 71 6.67e-23 96.7
Msa1188190 MtrunA17_Chr8g0367161 46.914 81 43 0 98 178 6 86 8.94e-23 99.0
Msa1188190 MtrunA17_Chr7g0237921 69.811 53 16 0 126 178 78 130 1.93e-22 98.2
Msa1188190 MtrunA17_Chr1g0188851 56.452 62 27 0 118 179 37 98 2.75e-22 96.7
Msa1188190 MtrunA17_Chr7g0223311 69.811 53 16 0 126 178 79 131 4.56e-22 97.4
Msa1188190 MtrunA17_Chr5g0411951 65.455 55 19 0 120 174 39 93 8.36e-22 95.9
Msa1188190 MtrunA17_Chr4g0047001 67.925 53 17 0 126 178 57 109 9.70e-22 96.7
Msa1188190 MtrunA17_Chr4g0030051 60.000 65 25 1 113 176 29 93 4.03e-21 93.2
Msa1188190 MtrunA17_Chr3g0117601 53.521 71 32 1 105 174 41 111 6.57e-21 93.6
Msa1188190 MtrunA17_Chr3g0126201 64.151 53 19 0 126 178 53 105 1.18e-20 92.8
Msa1188190 MtrunA17_Chr2g0282511 68.000 50 16 0 123 172 44 93 1.48e-20 92.0
Msa1188190 MtrunA17_Chr2g0282441 68.000 50 16 0 126 175 27 76 1.78e-20 92.0
Msa1188190 MtrunA17_Chr1g0177251 62.963 54 20 0 126 179 14 67 1.92e-20 91.3
Msa1188190 MtrunA17_Chr4g0047761 65.455 55 18 1 124 178 72 125 2.43e-20 91.7
Msa1188190 MtrunA17_Chr2g0282051 66.038 53 17 1 126 178 48 99 3.30e-20 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1188190 AT5G39660.3 36.765 408 184 17 77 454 93 456 1.81e-58 199
Msa1188190 AT5G39660.1 36.765 408 184 17 77 454 93 456 1.81e-58 199
Msa1188190 AT5G39660.2 36.765 408 184 17 77 454 93 456 1.81e-58 199
Msa1188190 AT3G47500.1 36.318 402 182 17 97 455 78 448 2.69e-56 193
Msa1188190 AT1G69570.1 30.676 414 171 13 75 455 67 397 7.86e-42 153
Msa1188190 AT5G62430.1 62.921 89 32 1 89 177 20 107 4.74e-34 129
Msa1188190 AT5G62430.1 41.000 100 46 3 361 455 207 298 1.44e-11 65.5
Msa1188190 AT1G29160.1 69.863 73 22 0 105 177 43 115 6.75e-34 125
Msa1188190 AT1G26790.1 30.645 372 156 16 101 455 86 372 8.57e-34 130
Msa1188190 AT2G34140.1 76.667 60 14 0 118 177 52 111 5.63e-32 120
Msa1188190 AT5G60850.1 75.000 56 14 0 124 179 51 106 7.12e-26 107
Msa1188190 AT3G55370.1 68.333 60 19 0 120 179 72 131 9.30e-25 104
Msa1188190 AT3G55370.2 68.333 60 19 0 120 179 72 131 1.04e-24 105
Msa1188190 AT3G55370.3 68.333 60 19 0 120 179 117 176 1.29e-24 105
Msa1188190 AT2G37590.1 65.000 60 21 0 120 179 85 144 2.73e-24 103
Msa1188190 AT1G51700.1 68.421 57 17 1 124 179 33 89 2.78e-24 100
Msa1188190 AT2G28810.2 69.643 56 17 0 120 175 73 128 4.55e-24 102
Msa1188190 AT2G28810.1 67.241 58 19 0 118 175 88 145 6.70e-24 102
Msa1188190 AT3G45610.1 50.633 79 34 1 106 179 17 95 7.85e-24 100
Msa1188190 AT5G60200.1 55.714 70 31 0 120 189 51 120 1.17e-23 100
Msa1188190 AT5G65590.1 51.685 89 40 1 91 179 11 96 1.32e-23 101
Msa1188190 AT1G07640.1 69.091 55 17 0 124 178 21 75 2.87e-23 99.4
Msa1188190 AT3G50410.1 50.000 80 30 1 109 178 5 84 2.93e-23 99.0
Msa1188190 AT4G38000.1 68.966 58 17 1 123 180 40 96 3.06e-23 98.6
Msa1188190 AT3G21270.1 69.811 53 16 0 124 176 29 81 3.34e-23 97.4
Msa1188190 AT1G07640.2 69.091 55 17 0 124 178 77 131 5.06e-23 99.8
Msa1188190 AT1G07640.3 69.091 55 17 0 124 178 85 139 6.76e-23 99.8
Msa1188190 AT4G24060.1 56.061 66 29 0 113 178 42 107 7.26e-23 99.8
Msa1188190 AT5G02460.1 66.102 59 20 0 120 178 91 149 8.18e-23 100
Msa1188190 AT3G61850.2 60.656 61 24 0 119 179 57 117 8.46e-23 98.2
Msa1188190 AT3G61850.3 60.656 61 24 0 119 179 57 117 8.46e-23 98.2
Msa1188190 AT3G61850.1 60.656 61 24 0 119 179 69 129 9.28e-23 98.6
Msa1188190 AT3G61850.5 60.656 61 24 0 119 179 57 117 1.34e-22 98.2
Msa1188190 AT3G61850.4 60.656 61 24 0 119 179 69 129 1.40e-22 98.6
Msa1188190 AT1G64620.1 61.667 60 23 0 113 172 38 97 2.30e-22 98.2
Msa1188190 AT4G21050.1 37.313 134 66 3 111 244 11 126 3.91e-22 94.7
Msa1188190 AT4G00940.1 50.000 78 39 0 119 196 63 140 4.36e-22 96.7
Msa1188190 AT4G00940.3 50.000 78 39 0 119 196 63 140 4.36e-22 96.7
Msa1188190 AT4G00940.2 50.000 78 39 0 119 196 63 140 4.36e-22 96.7
Msa1188190 AT2G28510.1 68.519 54 17 0 126 179 50 103 5.81e-22 95.9
Msa1188190 AT1G47655.1 69.388 49 15 0 124 172 29 77 1.44e-21 92.8
Msa1188190 AT5G66940.1 53.623 69 24 1 110 178 26 86 1.50e-21 93.2
Msa1188190 AT1G28310.1 65.455 55 19 0 124 178 27 81 2.01e-21 95.1
Msa1188190 AT1G28310.2 65.455 55 19 0 124 178 51 105 3.11e-21 94.7
Msa1188190 AT2G46590.1 64.151 53 19 0 119 171 63 115 1.59e-20 93.2
Msa1188190 AT2G46590.2 64.151 53 19 0 119 171 75 127 1.59e-20 93.2
Msa1188190 AT3G52440.1 62.264 53 20 0 126 178 27 79 3.03e-20 90.1
Msa1188190 AT3G52440.2 62.264 53 20 0 126 178 46 98 3.40e-20 90.5
Msa1188190 AT1G21340.1 61.111 54 21 0 126 179 40 93 5.50e-20 89.7
Msa1188190 AT5G62940.1 65.306 49 17 0 126 174 75 123 2.44e-19 89.7
Msa1188190 AT4G21040.1 67.347 49 16 0 126 174 27 75 5.30e-18 83.6
Msa1188190 AT4G21080.1 65.306 49 17 0 126 174 27 75 2.92e-17 81.6
Msa1188190 AT4G21030.1 50.769 65 25 2 112 169 2 66 2.06e-12 66.2

Find 99 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACGATGAAGCCGAATATT+TGG 0.219567 8_1:+28294741 Msa1188190:CDS
AAAACACTTCCTTTCATAAT+AGG 0.225913 8_1:-28294854 None:intergenic
TCCATTTGGCCAATCCAATT+TGG 0.234872 8_1:-28294587 None:intergenic
GAACTCTTAGCAGCCTCTTC+TGG 0.248749 8_1:-28294791 None:intergenic
ATTGGGGCTGTATGCCAAAT+TGG 0.283292 8_1:+28294573 Msa1188190:CDS
GGACTTGACGTTATGTTATC+AGG 0.299089 8_1:-28294509 None:intergenic
GGTAATAGGTCACCAACTTT+AGG 0.303102 8_1:+28294686 Msa1188190:CDS
TTGATTGTCAGTATCTCTTT+TGG 0.303295 8_1:-28293940 None:intergenic
GGACAGCTGGTGGAGTAATT+AGG 0.315052 8_1:+28294096 Msa1188190:CDS
TTGCTTATTTCTACGCTTAC+CGG 0.329821 8_1:-28294135 None:intergenic
ACCGATGACACATTCGTATT+GGG 0.335040 8_1:+28294556 Msa1188190:CDS
TCTGTGAGAGCTGCTGAATC+CGG 0.352345 8_1:+28294341 Msa1188190:CDS
GTGGTACTTGTTCCGGTAAT+AGG 0.368265 8_1:+28294672 Msa1188190:CDS
TTGTGGATTAGCTTGTTTGC+TGG 0.369067 8_1:-28293826 None:intergenic
ATTAGTTCTATGAGCTGCTT+TGG 0.372933 8_1:-28293904 None:intergenic
GCTAGCAGTGGTACTTGTTC+CGG 0.387977 8_1:+28294665 Msa1188190:CDS
AGCTGCTGAATCCGGGGAAA+TGG 0.391294 8_1:+28294349 Msa1188190:CDS
GCCAAATTGGATTGGCCAAA+TGG 0.393333 8_1:+28294586 Msa1188190:CDS
CTAACGATAATTCTTCCATT+TGG 0.399422 8_1:-28294601 None:intergenic
ATGGAAGAATTATCGTTAGT+TGG 0.407541 8_1:+28294605 Msa1188190:CDS
GTACCGGTATTGTACATGTT+TGG 0.410276 8_1:-28293867 None:intergenic
GTAATTAGGAATGTTCCTAT+TGG 0.410965 8_1:+28294110 Msa1188190:CDS
TTACAAAGTTGTTCCATTTG+AGG 0.416938 8_1:+28292495 Msa1188190:CDS
CTACGCTTACCGGCTCCAAT+AGG 0.417710 8_1:-28294125 None:intergenic
TACGATGAAGCCGAATATTT+GGG 0.418538 8_1:+28294742 Msa1188190:CDS
CGAGAACTTACATTTGACTT+AGG 0.422907 8_1:-28294890 None:intergenic
TAAAGTTGGTGACCTATTAC+CGG 0.424336 8_1:-28294684 None:intergenic
CTATGAGCTGCTTTGGTCAC+CGG 0.424624 8_1:-28293897 None:intergenic
TCTACAGCCATCATGGTTGC+AGG 0.425416 8_1:-28294530 None:intergenic
GGACACGGCAGAACCTTGTC+TGG 0.427129 8_1:-28293981 None:intergenic
AGCAGCTCTCACAGAAGAGC+TGG 0.431132 8_1:-28294333 None:intergenic
AATATAGAACATGCCTCAAA+TGG 0.433555 8_1:-28292508 None:intergenic
GACTTATCCATGTTGGAATC+CGG 0.435979 8_1:+28294475 Msa1188190:CDS
GTTTCTACCGATTCACTAAG+AGG 0.436362 8_1:-28294236 None:intergenic
CTGTGAGAGCTGCTGAATCC+GGG 0.445335 8_1:+28294342 Msa1188190:CDS
GTGTCCTCGCTGCAATAGTT+TGG 0.447623 8_1:+28293998 Msa1188190:CDS
TACCGATGACACATTCGTAT+TGG 0.449308 8_1:+28294555 Msa1188190:CDS
CACGCTTTCCTGAAAGGATC+CGG 0.450006 8_1:-28294966 None:intergenic
CATTCCATCACCTTCCTCTT+CGG 0.453115 8_1:+28294643 Msa1188190:CDS
CAATACCGGTACTTTGAAAC+CGG 0.464327 8_1:+28293878 Msa1188190:CDS
TGCCAGAGGTATTGGACAGC+TGG 0.480992 8_1:+28294083 Msa1188190:CDS
CCGGAGCGAGAGAAAGCAGC+AGG 0.481752 8_1:-28294947 None:intergenic
ATTATTACTCTGCTCCTCCA+TGG 0.483782 8_1:+28294453 Msa1188190:CDS
TTAGGAAAACATTGTAGAGA+TGG 0.504456 8_1:+28294704 Msa1188190:CDS
CGGTACTTCTACAGCCATCA+TGG 0.505901 8_1:-28294537 None:intergenic
GGAAGTTCCTCTTAGTGAAT+CGG 0.506714 8_1:+28294229 Msa1188190:CDS
AGGCTGCTAAGAGTTCTATA+TGG 0.510382 8_1:+28294798 Msa1188190:CDS
ACTAAGAGGAACTTCCTCCT+TGG 0.511294 8_1:-28294222 None:intergenic
CTCCATGGACTTATCCATGT+TGG 0.511657 8_1:+28294468 Msa1188190:CDS
CCTGCTGCTTTCTCTCGCTC+CGG 0.514923 8_1:+28294947 Msa1188190:CDS
TTCTGAATACTCCATTTCCC+CGG 0.521423 8_1:-28294360 None:intergenic
CACCAGCTGTCCAATACCTC+TGG 0.521554 8_1:-28294085 None:intergenic
GGCTGTATGCCAAATTGGAT+TGG 0.523809 8_1:+28294578 Msa1188190:CDS
GGTCACCGGTTTCAAAGTAC+CGG 0.526533 8_1:-28293883 None:intergenic
AGGAATGTTCCTATTGGAGC+CGG 0.533831 8_1:+28294116 Msa1188190:CDS
ATATTACACGCTTTCCTGAA+AGG 0.538533 8_1:-28294972 None:intergenic
TTAGTTGGATCTGCGTTCAA+CGG 0.546446 8_1:+28294620 Msa1188190:CDS
TTGGAATCTATGTGTATGAC+AGG 0.546648 8_1:-28294203 None:intergenic
AATGAAAAGCATGACCATGA+TGG 0.548748 8_1:-28292632 None:intergenic
ATTCCAAACATGTACAATAC+CGG 0.552268 8_1:+28293864 Msa1188190:CDS
TAATAATGAAGCGAATGTAA+AGG 0.553509 8_1:-28294437 None:intergenic
GAAATGGAGTATTCAGAAAG+TGG 0.559216 8_1:+28294365 Msa1188190:CDS
CAGAATTAATGATCCAGAAG+AGG 0.559620 8_1:+28294778 Msa1188190:CDS
TTATCAGGCTTAAATGCCGC+CGG 0.562271 8_1:-28294494 None:intergenic
TGTCATACACATAGATTCCA+AGG 0.569094 8_1:+28294205 Msa1188190:CDS
TATGACAGGTACTGCATCAA+GGG 0.571285 8_1:-28294189 None:intergenic
CAGAACAAACCTATTATGAA+AGG 0.575243 8_1:+28294845 Msa1188190:CDS
GTCAAGTCCTGCAACCATGA+TGG 0.576641 8_1:+28294523 Msa1188190:CDS
AAACTATTGCAGCGAGGACA+CGG 0.577128 8_1:-28293996 None:intergenic
ATAGTATTAGCATCTTCTTG+TGG 0.578337 8_1:-28293843 None:intergenic
GAAAGTGGAATAGAGCATGT+TGG 0.578755 8_1:+28294380 Msa1188190:CDS
CCCCAATACGAATGTGTCAT+CGG 0.582026 8_1:-28294557 None:intergenic
ACTTTGACACCACAATGCAA+TGG 0.588024 8_1:+28294407 Msa1188190:CDS
TTCCAACATGGATAAGTCCA+TGG 0.593931 8_1:-28294470 None:intergenic
GTATGACAGGTACTGCATCA+AGG 0.597457 8_1:-28294190 None:intergenic
TCCACATCAACAATCCATCA+TGG 0.598956 8_1:+28292618 Msa1188190:CDS
ACCATGATGGATTGTTGATG+TGG 0.602567 8_1:-28292619 None:intergenic
AGTACCACTGCTAGCCGAAG+AGG 0.608123 8_1:-28294657 None:intergenic
CTAGCCGAAGAGGAAGGTGA+TGG 0.608712 8_1:-28294647 None:intergenic
CCGATGACACATTCGTATTG+GGG 0.610193 8_1:+28294557 Msa1188190:CDS
CCTTCCTCTTCGGCTAGCAG+TGG 0.612556 8_1:+28294653 Msa1188190:CDS
AGAAACTGTGTTAAATCACA+AGG 0.612750 8_1:+28294253 Msa1188190:CDS
CAGAGGTATTGGACAGCTGG+TGG 0.613246 8_1:+28294086 Msa1188190:CDS
ATTACAATGTTAACCAACCA+AGG 0.617440 8_1:+28294045 Msa1188190:CDS
GGACTCTTCTACTGTCAAAG+AGG 0.620817 8_1:+28294295 Msa1188190:CDS
GGAAGAGAGTGAATCTGTGG+TGG 0.622672 8_1:-28292598 None:intergenic
TGTGAGAGCTGCTGAATCCG+GGG 0.623039 8_1:+28294343 Msa1188190:CDS
AGAACTTGACAATTCTGCAA+AGG 0.627470 8_1:-28294161 None:intergenic
GGAATTAAGCCATTGCATTG+TGG 0.632188 8_1:-28294416 None:intergenic
GAAGAGAGTGAATCTGTGGT+GGG 0.633316 8_1:-28292597 None:intergenic
AATGCCGCCGGATTCCAACA+TGG 0.640756 8_1:-28294482 None:intergenic
CGGAGCGAGAGAAAGCAGCA+GGG 0.655440 8_1:-28294946 None:intergenic
TTATCCATGTTGGAATCCGG+CGG 0.663952 8_1:+28294478 Msa1188190:CDS
CCACTGCTAGCCGAAGAGGA+AGG 0.690209 8_1:-28294653 None:intergenic
TGTGGAAGAGAGTGAATCTG+TGG 0.693535 8_1:-28292601 None:intergenic
GTCTCCAAACTATTGCAGCG+AGG 0.702604 8_1:-28294002 None:intergenic
GAAACTGTGTTAAATCACAA+GGG 0.705716 8_1:+28294254 Msa1188190:CDS
CAACATGGATAAGTCCATGG+AGG 0.737932 8_1:-28294467 None:intergenic
CATACACATAGATTCCAAGG+AGG 0.765853 8_1:+28294208 Msa1188190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATAAGAAAGATAAAA+AGG - chr8_1:28292679-28292698 None:intergenic 10.0%
!!! TATCTTTCTTATATATTTTT+TGG + chr8_1:28292682-28292701 Msa1188190:intron 10.0%
!! AAATTAAAATGAAATATGTT+AGG + chr8_1:28293310-28293329 Msa1188190:intron 10.0%
!! AAAATGTTTATATTATAGCT+AGG + chr8_1:28292893-28292912 Msa1188190:intron 15.0%
!!! TTTGAAAAAGTCATTATTTT+CGG + chr8_1:28293628-28293647 Msa1188190:intron 15.0%
!! TGAAAGGAAAAAAAAAATCT+GGG - chr8_1:28292761-28292780 None:intergenic 20.0%
!! ATGAAAGGAAAAAAAAAATC+TGG - chr8_1:28292762-28292781 None:intergenic 20.0%
!! ATTCGTATATATATAGTTCT+AGG + chr8_1:28292812-28292831 Msa1188190:intron 20.0%
!!! TGTTTGATAATTTGTTTTGA+AGG + chr8_1:28292926-28292945 Msa1188190:intron 20.0%
!!! GAATTATTTTTGTTTGAATC+TGG + chr8_1:28293005-28293024 Msa1188190:intron 20.0%
!! TGACATGAAAAATGTTAAAT+TGG + chr8_1:28293063-28293082 Msa1188190:intron 20.0%
!! TTTGAACTTAACATAACAAA+AGG - chr8_1:28293353-28293372 None:intergenic 20.0%
!! TAATCAAACAGTTAAAGAAT+AGG - chr8_1:28293407-28293426 None:intergenic 20.0%
!!! TTTTGGTATTGTTTAATTTG+TGG + chr8_1:28293451-28293470 Msa1188190:intron 20.0%
!!! TTTGGTATTGTTTAATTTGT+GGG + chr8_1:28293452-28293471 Msa1188190:intron 20.0%
!!! TAATGACTTTTTCAAATTAG+CGG - chr8_1:28293624-28293643 None:intergenic 20.0%
!!! TATCAATGAGTTATAACTTT+TGG + chr8_1:28293687-28293706 Msa1188190:intron 20.0%
!!! TCATTAATTCTGATAGTTTT+CGG - chr8_1:28294770-28294789 None:intergenic 20.0%
!!! TTTTTTCCTTTCATACTGAA+TGG + chr8_1:28292768-28292787 Msa1188190:intron 25.0%
!! GTGTTACTATAGAAAATAAG+TGG + chr8_1:28292969-28292988 Msa1188190:intron 25.0%
! TACTATAGAAAATAAGTGGT+AGG + chr8_1:28292973-28292992 Msa1188190:intron 25.0%
! ACTATAGAAAATAAGTGGTA+GGG + chr8_1:28292974-28292993 Msa1188190:intron 25.0%
!!! TTGAATCTGGTTTTTTAAGT+TGG + chr8_1:28293018-28293037 Msa1188190:intron 25.0%
!! ACATTTTTCATGTCAATTAC+TGG - chr8_1:28293058-28293077 None:intergenic 25.0%
! AAATTGGACCAAATTAATTG+AGG + chr8_1:28293079-28293098 Msa1188190:intron 25.0%
! ATAATTTGAGTCCAATTACT+CGG - chr8_1:28293145-28293164 None:intergenic 25.0%
!! TTAATTTTGTGTTAATCCTC+TGG - chr8_1:28293297-28293316 None:intergenic 25.0%
! AAATCAACGTTGTTAAATAG+TGG + chr8_1:28293502-28293521 Msa1188190:intron 25.0%
! TTGTACTAGATAATGTATTG+TGG - chr8_1:28293565-28293584 None:intergenic 25.0%
! TTAAAAAGACAACTTGCATT+AGG - chr8_1:28293714-28293733 None:intergenic 25.0%
! TAATAATGAAGCGAATGTAA+AGG - chr8_1:28294440-28294459 None:intergenic 25.0%
! AAAACACTTCCTTTCATAAT+AGG - chr8_1:28294857-28294876 None:intergenic 25.0%
TTACAAAGTTGTTCCATTTG+AGG + chr8_1:28292495-28292514 Msa1188190:CDS 30.0%
AATATAGAACATGCCTCAAA+TGG - chr8_1:28292511-28292530 None:intergenic 30.0%
AAGGTGCAAAAAAGATGTAA+AGG - chr8_1:28292660-28292679 None:intergenic 30.0%
CTATATCCATTCAGTATGAA+AGG - chr8_1:28292777-28292796 None:intergenic 30.0%
TTCATACTGAATGGATATAG+AGG + chr8_1:28292777-28292796 Msa1188190:intron 30.0%
AGAACACACCTCAATTAATT+TGG - chr8_1:28293090-28293109 None:intergenic 30.0%
AAATTAATTGAGGTGTGTTC+TGG + chr8_1:28293089-28293108 Msa1188190:intron 30.0%
TAAACAATACCAAAAACTCG+TGG - chr8_1:28293446-28293465 None:intergenic 30.0%
ATTGTGGAACAACACTAATT+TGG - chr8_1:28293549-28293568 None:intergenic 30.0%
! TTGATAGTATGCATTTCTAC+CGG - chr8_1:28293673-28293692 None:intergenic 30.0%
ATAGTATTAGCATCTTCTTG+TGG - chr8_1:28293846-28293865 None:intergenic 30.0%
ATTCCAAACATGTACAATAC+CGG + chr8_1:28293864-28293883 Msa1188190:CDS 30.0%
! TTGATTGTCAGTATCTCTTT+TGG - chr8_1:28293943-28293962 None:intergenic 30.0%
ATTACAATGTTAACCAACCA+AGG + chr8_1:28294045-28294064 Msa1188190:CDS 30.0%
GTAATTAGGAATGTTCCTAT+TGG + chr8_1:28294110-28294129 Msa1188190:CDS 30.0%
AGAAACTGTGTTAAATCACA+AGG + chr8_1:28294253-28294272 Msa1188190:CDS 30.0%
GAAACTGTGTTAAATCACAA+GGG + chr8_1:28294254-28294273 Msa1188190:CDS 30.0%
! AGTCCAGTTCAATTTTTCTA+TGG - chr8_1:28294280-28294299 None:intergenic 30.0%
CTAACGATAATTCTTCCATT+TGG - chr8_1:28294604-28294623 None:intergenic 30.0%
ATGGAAGAATTATCGTTAGT+TGG + chr8_1:28294605-28294624 Msa1188190:CDS 30.0%
! CTACAATGTTTTCCTAAAGT+TGG - chr8_1:28294701-28294720 None:intergenic 30.0%
TTAGGAAAACATTGTAGAGA+TGG + chr8_1:28294704-28294723 Msa1188190:CDS 30.0%
CAGAACAAACCTATTATGAA+AGG + chr8_1:28294845-28294864 Msa1188190:CDS 30.0%
! GATCCTTCAATCAAGCTTTT+TGG + chr8_1:28292552-28292571 Msa1188190:CDS 35.0%
CTTCCAAAAAGCTTGATTGA+AGG - chr8_1:28292558-28292577 None:intergenic 35.0%
! AATGAAAAGCATGACCATGA+TGG - chr8_1:28292635-28292654 None:intergenic 35.0%
! TGTTCTGGTTACTGTATCAT+TGG + chr8_1:28293104-28293123 Msa1188190:intron 35.0%
CTGTATCATTGGAATATGTC+TGG + chr8_1:28293115-28293134 Msa1188190:intron 35.0%
ACTAAGTACGAATTGTTCGT+GGG + chr8_1:28293176-28293195 Msa1188190:intron 35.0%
! GTCAGACTTTACTTACGTTT+TGG + chr8_1:28293234-28293253 Msa1188190:intron 35.0%
TTTGGTACTCAATTGCAGAA+TGG + chr8_1:28293753-28293772 Msa1188190:intron 35.0%
!! ATTAGTTCTATGAGCTGCTT+TGG - chr8_1:28293907-28293926 None:intergenic 35.0%
! CAATTTTTGCAGAAATGCCT+TGG - chr8_1:28294065-28294084 None:intergenic 35.0%
ATTTCTGCAAAAATTGCCAG+AGG + chr8_1:28294069-28294088 Msa1188190:CDS 35.0%
!! TTGCTTATTTCTACGCTTAC+CGG - chr8_1:28294138-28294157 None:intergenic 35.0%
AGAACTTGACAATTCTGCAA+AGG - chr8_1:28294164-28294183 None:intergenic 35.0%
TTGGAATCTATGTGTATGAC+AGG - chr8_1:28294206-28294225 None:intergenic 35.0%
TGTCATACACATAGATTCCA+AGG + chr8_1:28294205-28294224 Msa1188190:CDS 35.0%
! GAAATGGAGTATTCAGAAAG+TGG + chr8_1:28294365-28294384 Msa1188190:CDS 35.0%
!! TAAAGTTGGTGACCTATTAC+CGG - chr8_1:28294687-28294706 None:intergenic 35.0%
ATACGATGAAGCCGAATATT+TGG + chr8_1:28294741-28294760 Msa1188190:CDS 35.0%
TACGATGAAGCCGAATATTT+GGG + chr8_1:28294742-28294761 Msa1188190:CDS 35.0%
CAGAATTAATGATCCAGAAG+AGG + chr8_1:28294778-28294797 Msa1188190:CDS 35.0%
! CTGTTCAGATTTTGTTCTCA+AGG - chr8_1:28294828-28294847 None:intergenic 35.0%
! CGAGAACTTACATTTGACTT+AGG - chr8_1:28294893-28294912 None:intergenic 35.0%
TCCACATCAACAATCCATCA+TGG + chr8_1:28292618-28292637 Msa1188190:CDS 40.0%
!! ACCATGATGGATTGTTGATG+TGG - chr8_1:28292622-28292641 None:intergenic 40.0%
CACGAACAATTCGTACTTAG+TGG - chr8_1:28293177-28293196 None:intergenic 40.0%
CACTAAGTACGAATTGTTCG+TGG + chr8_1:28293175-28293194 Msa1188190:intron 40.0%
TTCCTGAGTGGGATAATTCT+TGG + chr8_1:28293212-28293231 Msa1188190:intron 40.0%
! GTTAATCCTCTGGTTCCTAA+GGG - chr8_1:28293287-28293306 None:intergenic 40.0%
! TGTTAATCCTCTGGTTCCTA+AGG - chr8_1:28293288-28293307 None:intergenic 40.0%
! CGAGTAAAACCACGAGTTTT+TGG + chr8_1:28293434-28293453 Msa1188190:intron 40.0%
TTAGCGGCATAACAGTTCTA+TGG - chr8_1:28293608-28293627 None:intergenic 40.0%
GTATGTCAACTGACACAGTT+TGG + chr8_1:28293735-28293754 Msa1188190:intron 40.0%
!! TTGTGGATTAGCTTGTTTGC+TGG - chr8_1:28293829-28293848 None:intergenic 40.0%
! GTACCGGTATTGTACATGTT+TGG - chr8_1:28293870-28293889 None:intergenic 40.0%
!! CAATACCGGTACTTTGAAAC+CGG + chr8_1:28293878-28293897 Msa1188190:CDS 40.0%
!! AGTTTTCAAGAAGCCAGACA+AGG + chr8_1:28293968-28293987 Msa1188190:CDS 40.0%
! TTTTGCAGAAATGCCTTGGT+TGG - chr8_1:28294061-28294080 None:intergenic 40.0%
! GCAAAAATTGCCAGAGGTAT+TGG + chr8_1:28294075-28294094 Msa1188190:CDS 40.0%
! TATGACAGGTACTGCATCAA+GGG - chr8_1:28294192-28294211 None:intergenic 40.0%
CATACACATAGATTCCAAGG+AGG + chr8_1:28294208-28294227 Msa1188190:CDS 40.0%
GGAAGTTCCTCTTAGTGAAT+CGG + chr8_1:28294229-28294248 Msa1188190:CDS 40.0%
!! GTTTCTACCGATTCACTAAG+AGG - chr8_1:28294239-28294258 None:intergenic 40.0%
GGGCCATAGAAAAATTGAAC+TGG + chr8_1:28294274-28294293 Msa1188190:CDS 40.0%
TTCTGAATACTCCATTTCCC+CGG - chr8_1:28294363-28294382 None:intergenic 40.0%
GAAAGTGGAATAGAGCATGT+TGG + chr8_1:28294380-28294399 Msa1188190:CDS 40.0%
ACTTTGACACCACAATGCAA+TGG + chr8_1:28294407-28294426 Msa1188190:CDS 40.0%
GGAATTAAGCCATTGCATTG+TGG - chr8_1:28294419-28294438 None:intergenic 40.0%
ATTATTACTCTGCTCCTCCA+TGG + chr8_1:28294453-28294472 Msa1188190:CDS 40.0%
TTCCAACATGGATAAGTCCA+TGG - chr8_1:28294473-28294492 None:intergenic 40.0%
GACTTATCCATGTTGGAATC+CGG + chr8_1:28294475-28294494 Msa1188190:CDS 40.0%
GGACTTGACGTTATGTTATC+AGG - chr8_1:28294512-28294531 None:intergenic 40.0%
TACCGATGACACATTCGTAT+TGG + chr8_1:28294555-28294574 Msa1188190:CDS 40.0%
ACCGATGACACATTCGTATT+GGG + chr8_1:28294556-28294575 Msa1188190:CDS 40.0%
TCCATTTGGCCAATCCAATT+TGG - chr8_1:28294590-28294609 None:intergenic 40.0%
TTAGTTGGATCTGCGTTCAA+CGG + chr8_1:28294620-28294639 Msa1188190:CDS 40.0%
GGTAATAGGTCACCAACTTT+AGG + chr8_1:28294686-28294705 Msa1188190:CDS 40.0%
! GTTTTCGGCACCCAAATATT+CGG - chr8_1:28294755-28294774 None:intergenic 40.0%
AGGCTGCTAAGAGTTCTATA+TGG + chr8_1:28294798-28294817 Msa1188190:CDS 40.0%
GAAGAGAGTGAATCTGTGGT+GGG - chr8_1:28292600-28292619 None:intergenic 45.0%
TGTGGAAGAGAGTGAATCTG+TGG - chr8_1:28292604-28292623 None:intergenic 45.0%
TGTCTGGTTGACCGAGTAAT+TGG + chr8_1:28293131-28293150 Msa1188190:intron 45.0%
ATCTGAGTTCGATTCCTGAG+TGG + chr8_1:28293200-28293219 Msa1188190:intron 45.0%
TCTGAGTTCGATTCCTGAGT+GGG + chr8_1:28293201-28293220 Msa1188190:intron 45.0%
GACCAAGAATTATCCCACTC+AGG - chr8_1:28293217-28293236 None:intergenic 45.0%
TCTACCGGTGCTTTCAATCA+CGG - chr8_1:28293658-28293677 None:intergenic 45.0%
AAACTATTGCAGCGAGGACA+CGG - chr8_1:28293999-28294018 None:intergenic 45.0%
AGGAATGTTCCTATTGGAGC+CGG + chr8_1:28294116-28294135 Msa1188190:CDS 45.0%
! GTATGACAGGTACTGCATCA+AGG - chr8_1:28294193-28294212 None:intergenic 45.0%
ACTAAGAGGAACTTCCTCCT+TGG - chr8_1:28294225-28294244 None:intergenic 45.0%
GGACTCTTCTACTGTCAAAG+AGG + chr8_1:28294295-28294314 Msa1188190:CDS 45.0%
CAACATGGATAAGTCCATGG+AGG - chr8_1:28294470-28294489 None:intergenic 45.0%
CTCCATGGACTTATCCATGT+TGG + chr8_1:28294468-28294487 Msa1188190:CDS 45.0%
TTATCCATGTTGGAATCCGG+CGG + chr8_1:28294478-28294497 Msa1188190:CDS 45.0%
TTATCAGGCTTAAATGCCGC+CGG - chr8_1:28294497-28294516 None:intergenic 45.0%
CCCCAATACGAATGTGTCAT+CGG - chr8_1:28294560-28294579 None:intergenic 45.0%
CCGATGACACATTCGTATTG+GGG + chr8_1:28294557-28294576 Msa1188190:CDS 45.0%
ATTGGGGCTGTATGCCAAAT+TGG + chr8_1:28294573-28294592 Msa1188190:CDS 45.0%
GGCTGTATGCCAAATTGGAT+TGG + chr8_1:28294578-28294597 Msa1188190:CDS 45.0%
!! GCCAAATTGGATTGGCCAAA+TGG + chr8_1:28294586-28294605 Msa1188190:CDS 45.0%
CATTCCATCACCTTCCTCTT+CGG + chr8_1:28294643-28294662 Msa1188190:CDS 45.0%
GTGGTACTTGTTCCGGTAAT+AGG + chr8_1:28294672-28294691 Msa1188190:CDS 45.0%
GGAAGAGAGTGAATCTGTGG+TGG - chr8_1:28292601-28292620 None:intergenic 50.0%
!! CTTACGTTTTGGCCGAACTC+CGG + chr8_1:28293245-28293264 Msa1188190:intron 50.0%
CGTTCCAGTAATCCGGAGTT+CGG - chr8_1:28293260-28293279 None:intergenic 50.0%
TTACTGGAACGCCTTCCCTT+AGG + chr8_1:28293269-28293288 Msa1188190:intron 50.0%
! ATCCTCTGGTTCCTAAGGGA+AGG - chr8_1:28293283-28293302 None:intergenic 50.0%
! CGATCCGTGATTGAAAGCAC+CGG + chr8_1:28293651-28293670 Msa1188190:intron 50.0%
!! GGTCACCGGTTTCAAAGTAC+CGG - chr8_1:28293886-28293905 None:intergenic 50.0%
! CTATGAGCTGCTTTGGTCAC+CGG - chr8_1:28293900-28293919 None:intergenic 50.0%
GTGTCCTCGCTGCAATAGTT+TGG + chr8_1:28293998-28294017 Msa1188190:CDS 50.0%
GTCTCCAAACTATTGCAGCG+AGG - chr8_1:28294005-28294024 None:intergenic 50.0%
GGACAGCTGGTGGAGTAATT+AGG + chr8_1:28294096-28294115 Msa1188190:CDS 50.0%
TCTGTGAGAGCTGCTGAATC+CGG + chr8_1:28294341-28294360 Msa1188190:CDS 50.0%
GTCAAGTCCTGCAACCATGA+TGG + chr8_1:28294523-28294542 Msa1188190:CDS 50.0%
TCTACAGCCATCATGGTTGC+AGG - chr8_1:28294533-28294552 None:intergenic 50.0%
CGGTACTTCTACAGCCATCA+TGG - chr8_1:28294540-28294559 None:intergenic 50.0%
GCTAGCAGTGGTACTTGTTC+CGG + chr8_1:28294665-28294684 Msa1188190:CDS 50.0%
GAACTCTTAGCAGCCTCTTC+TGG - chr8_1:28294794-28294813 None:intergenic 50.0%
CACGCTTTCCTGAAAGGATC+CGG - chr8_1:28294969-28294988 None:intergenic 50.0%
TTGGCCGAACTCCGGATTAC+TGG + chr8_1:28293253-28293272 Msa1188190:intron 55.0%
!! GGGAAGGCGTTCCAGTAATC+CGG - chr8_1:28293267-28293286 None:intergenic 55.0%
!! TGCCAGAGGTATTGGACAGC+TGG + chr8_1:28294083-28294102 Msa1188190:CDS 55.0%
CACCAGCTGTCCAATACCTC+TGG - chr8_1:28294088-28294107 None:intergenic 55.0%
!! CAGAGGTATTGGACAGCTGG+TGG + chr8_1:28294086-28294105 Msa1188190:CDS 55.0%
!! CTACGCTTACCGGCTCCAAT+AGG - chr8_1:28294128-28294147 None:intergenic 55.0%
AGCAGCTCTCACAGAAGAGC+TGG - chr8_1:28294336-28294355 None:intergenic 55.0%
CTGTGAGAGCTGCTGAATCC+GGG + chr8_1:28294342-28294361 Msa1188190:CDS 55.0%
TGTGAGAGCTGCTGAATCCG+GGG + chr8_1:28294343-28294362 Msa1188190:CDS 55.0%
AGCTGCTGAATCCGGGGAAA+TGG + chr8_1:28294349-28294368 Msa1188190:CDS 55.0%
AATGCCGCCGGATTCCAACA+TGG - chr8_1:28294485-28294504 None:intergenic 55.0%
CTAGCCGAAGAGGAAGGTGA+TGG - chr8_1:28294650-28294669 None:intergenic 55.0%
AGTACCACTGCTAGCCGAAG+AGG - chr8_1:28294660-28294679 None:intergenic 55.0%
CGCCTTCCCTTAGGAACCAG+AGG + chr8_1:28293278-28293297 Msa1188190:intron 60.0%
GGACACGGCAGAACCTTGTC+TGG - chr8_1:28293984-28294003 None:intergenic 60.0%
CCACTGCTAGCCGAAGAGGA+AGG - chr8_1:28294656-28294675 None:intergenic 60.0%
CCTTCCTCTTCGGCTAGCAG+TGG + chr8_1:28294653-28294672 Msa1188190:CDS 60.0%
CGGAGCGAGAGAAAGCAGCA+GGG - chr8_1:28294949-28294968 None:intergenic 60.0%
!! CCTGCTGCTTTCTCTCGCTC+CGG + chr8_1:28294947-28294966 Msa1188190:CDS 60.0%
CTCTCGCTCCGGATCCTTTC+AGG + chr8_1:28294958-28294977 Msa1188190:CDS 60.0%
CCGGAGCGAGAGAAAGCAGC+AGG - chr8_1:28294950-28294969 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 28292493 28294988 28292493 ID=Msa1188190;Name=Msa1188190
chr8_1 mRNA 28292493 28294988 28292493 ID=Msa1188190-mRNA-1;Parent=Msa1188190;Name=Msa1188190-mRNA-1;_AED=0.05;_eAED=0.07;_QI=0|1|0|1|0|0|2|0|455
chr8_1 exon 28292493 28292639 28292493 ID=Msa1188190-mRNA-1:exon:9964;Parent=Msa1188190-mRNA-1
chr8_1 exon 28293771 28294988 28293771 ID=Msa1188190-mRNA-1:exon:9965;Parent=Msa1188190-mRNA-1
chr8_1 CDS 28292493 28292639 28292493 ID=Msa1188190-mRNA-1:cds;Parent=Msa1188190-mRNA-1
chr8_1 CDS 28293771 28294988 28293771 ID=Msa1188190-mRNA-1:cds;Parent=Msa1188190-mRNA-1
Gene Sequence

>Msa1188190

ACTTACAAAGTTGTTCCATTTGAGGCATGTTCTATATTAAACAAGAACAACAAGAAGAAGATCCTTCAATCAAGCTTTTTGGAAGTGATATTCAAATTCATACTCCCACCACAGATTCACTCTCTTCCACATCAACAATCCATCATGAATGGACTTGAAGAATCAACTAATGCTGATGTTGATGTTCGTATGAATGAAGTTTCCAGCAAACAAGCTAATCCACAAGAAGATGCTAATACTATTCCAAACATGTACAATACCGGTACTTTGAAACCGGTGACCAAAGCAGCTCATAGAACTAATGAACACAAGACTGCCAAAAGAGATACTGACAATCAAGAGAAAGTTTTCAAGAAGCCAGACAAGGTTCTGCCGTGTCCTCGCTGCAATAGTTTGGAGACAAAGTTTTGCTATTTCAACAATTACAATGTTAACCAACCAAGGCATTTCTGCAAAAATTGCCAGAGGTATTGGACAGCTGGTGGAGTAATTAGGAATGTTCCTATTGGAGCCGGTAAGCGTAGAAATAAGCAATCACCTTTGCAGAATTGTCAAGTTCTTGTTACCCTTGATGCAGTACCTGTCATACACATAGATTCCAAGGAGGAAGTTCCTCTTAGTGAATCGGTAGAAACTGTGTTAAATCACAAGGGCCATAGAAAAATTGAACTGGACTCTTCTACTGTCAAAGAGGATATTGAGAACTCTTCCAGCTCTTCTGTGAGAGCTGCTGAATCCGGGGAAATGGAGTATTCAGAAAGTGGAATAGAGCATGTTGGTTTGACTTTGACACCACAATGCAATGGCTTAATTCCTTTACATTCGCTTCATTATTACTCTGCTCCTCCATGGACTTATCCATGTTGGAATCCGGCGGCATTTAAGCCTGATAACATAACGTCAAGTCCTGCAACCATGATGGCTGTAGAAGTACCGATGACACATTCGTATTGGGGCTGTATGCCAAATTGGATTGGCCAAATGGAAGAATTATCGTTAGTTGGATCTGCGTTCAACGGCATTCCATCACCTTCCTCTTCGGCTAGCAGTGGTACTTGTTCCGGTAATAGGTCACCAACTTTAGGAAAACATTGTAGAGATGGAAGTACACAAGAAGATACGATGAAGCCGAATATTTGGGTGCCGAAAACTATCAGAATTAATGATCCAGAAGAGGCTGCTAAGAGTTCTATATGGTCAACCTTGAGAACAAAATCTGAACAGAACAAACCTATTATGAAAGGAAGTGTTTTCAAATCATTTGAACCTAAGTCAAATGTAAGTTCTCGTGATTTAGACGATAATCAAATTCTAAGAGCAAACCCTGCTGCTTTCTCTCGCTCCGGATCCTTTCAGGAAAGCGTG
Protein sequence

>Msa1188190

TYKVVPFEACSILNKNNKKKILQSSFLEVIFKFILPPQIHSLPHQQSIMNGLEESTNADVDVRMNEVSSKQANPQEDANTIPNMYNTGTLKPVTKAAHRTNEHKTAKRDTDNQEKVFKKPDKVLPCPRCNSLETKFCYFNNYNVNQPRHFCKNCQRYWTAGGVIRNVPIGAGKRRNKQSPLQNCQVLVTLDAVPVIHIDSKEEVPLSESVETVLNHKGHRKIELDSSTVKEDIENSSSSSVRAAESGEMEYSESGIEHVGLTLTPQCNGLIPLHSLHYYSAPPWTYPCWNPAAFKPDNITSSPATMMAVEVPMTHSYWGCMPNWIGQMEELSLVGSAFNGIPSPSSSASSGTCSGNRSPTLGKHCRDGSTQEDTMKPNIWVPKTIRINDPEEAAKSSIWSTLRTKSEQNKPIMKGSVFKSFEPKSNVSSRDLDDNQILRANPAAFSRSGSFQESV