Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1189280 | XP_013445128.1 | 73.394 | 109 | 26 | 1 | 14 | 119 | 21 | 129 | 7.02e-50 | 165 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1189280 | sp|Q9M9S0|ZHD4_ARATH | 32.624 | 141 | 61 | 4 | 15 | 121 | 107 | 247 | 1.89e-12 | 65.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa1189280 | TF | zf-HD |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1189280 | MtrunA17_Chr8g0357721 | 73.394 | 109 | 26 | 1 | 14 | 119 | 21 | 129 | 6.46e-54 | 165 |
| Msa1189280 | MtrunA17_Chr8g0357741 | 90.323 | 62 | 6 | 0 | 58 | 119 | 1 | 62 | 2.03e-35 | 117 |
| Msa1189280 | MtrunA17_Chr2g0315941 | 38.514 | 148 | 48 | 6 | 14 | 118 | 84 | 231 | 2.70e-19 | 80.5 |
| Msa1189280 | MtrunA17_Chr8g0367691 | 38.065 | 155 | 44 | 7 | 14 | 121 | 85 | 234 | 9.42e-19 | 79.0 |
| Msa1189280 | MtrunA17_Chr5g0404271 | 28.025 | 157 | 53 | 4 | 13 | 119 | 114 | 260 | 2.46e-14 | 67.4 |
| Msa1189280 | MtrunA17_Chr1g0188441 | 28.125 | 128 | 69 | 2 | 14 | 119 | 25 | 151 | 2.17e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1189280 | AT1G14440.1 | 32.624 | 141 | 61 | 4 | 15 | 121 | 107 | 247 | 1.92e-13 | 65.5 |
| Msa1189280 | AT1G14440.2 | 32.624 | 141 | 61 | 4 | 15 | 121 | 107 | 247 | 1.92e-13 | 65.5 |
| Msa1189280 | AT2G18350.1 | 29.323 | 133 | 62 | 3 | 13 | 118 | 97 | 224 | 4.16e-12 | 61.2 |
Find 27 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGATCCTCCATTGAAATT+TGG | 0.135463 | 8_1:-30723948 | None:intergenic |
| TCTTGTCATAACTTCATCTA+TGG | 0.262086 | 8_1:+30723921 | Msa1189280:CDS |
| CGAAATCGAATAAGAGGTTT+TGG | 0.329727 | 8_1:+30724034 | Msa1189280:CDS |
| TTGGATCTTGCTATGACATT+AGG | 0.351262 | 8_1:+30724090 | Msa1189280:CDS |
| CAAGAAGTAGCCTCCAATAT+TGG | 0.357708 | 8_1:-30723789 | None:intergenic |
| TATTGGAGGCTACTTCTTGA+TGG | 0.372242 | 8_1:+30723793 | Msa1189280:CDS |
| CCAGCACCATCACCATTGAT+AGG | 0.458035 | 8_1:-30724000 | None:intergenic |
| CATCTTCTTCCTTTGCTCAC+GGG | 0.472025 | 8_1:-30724067 | None:intergenic |
| ACATCTTCTTCCTTTGCTCA+CGG | 0.486999 | 8_1:-30724068 | None:intergenic |
| GGACGTCGAAATCGAATAAG+AGG | 0.494204 | 8_1:+30724028 | Msa1189280:CDS |
| TATGGGGATCCAAATTTCAA+TGG | 0.497183 | 8_1:+30723939 | Msa1189280:CDS |
| CCTATCAATGGTGATGGTGC+TGG | 0.500146 | 8_1:+30724000 | Msa1189280:CDS |
| GTTATGACAAGACTAGTTGC+AGG | 0.514220 | 8_1:-30723910 | None:intergenic |
| AGACATATTGAGCCTATCAA+TGG | 0.544287 | 8_1:+30723988 | Msa1189280:CDS |
| GTGGTACCTTCGGCTCCAGC+GGG | 0.546226 | 8_1:-30723832 | None:intergenic |
| CTTGTCATAACTTCATCTAT+GGG | 0.547890 | 8_1:+30723922 | Msa1189280:CDS |
| AATGGTGATGGTGCTGGAGA+AGG | 0.551509 | 8_1:+30724006 | Msa1189280:CDS |
| ATCTTGCTATGACATTAGGT+TGG | 0.558400 | 8_1:+30724094 | Msa1189280:CDS |
| GGTGGTACCTTCGGCTCCAG+CGG | 0.577457 | 8_1:-30723833 | None:intergenic |
| TGAGCAAAGGAAGAAGATGT+TGG | 0.584392 | 8_1:+30724071 | Msa1189280:CDS |
| ATGGTGATGGTGCTGGAGAA+GGG | 0.586074 | 8_1:+30724007 | Msa1189280:CDS |
| CAAAGTTTACCCGTGAGCAA+AGG | 0.600303 | 8_1:+30724058 | Msa1189280:CDS |
| AATCACATGTCCAATATTGG+AGG | 0.604304 | 8_1:+30723779 | Msa1189280:CDS |
| GGGGATCCAAATTTCAATGG+AGG | 0.640010 | 8_1:+30723942 | Msa1189280:CDS |
| TAGTTGCAGGTACAATACAA+TGG | 0.652775 | 8_1:-30723897 | None:intergenic |
| ATTGAGCCTATCAATGGTGA+TGG | 0.658220 | 8_1:+30723994 | Msa1189280:CDS |
| TTGTCATAACTTCATCTATG+GGG | 0.673616 | 8_1:+30723923 | Msa1189280:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAACATTTAAAAAAAATGG+TGG | - | chr8_1:30723854-30723873 | None:intergenic | 20.0% |
| !! | AGCAGAACATTTAAAAAAAA+TGG | - | chr8_1:30723857-30723876 | None:intergenic | 20.0% |
| ! | AAAAATCACATGTCCAATAT+TGG | + | chr8_1:30723776-30723795 | Msa1189280:CDS | 25.0% |
| AAAAAAAATGGTGGTACCTT+CGG | - | chr8_1:30723845-30723864 | None:intergenic | 30.0% | |
| TCTTGTCATAACTTCATCTA+TGG | + | chr8_1:30723921-30723940 | Msa1189280:CDS | 30.0% | |
| CTTGTCATAACTTCATCTAT+GGG | + | chr8_1:30723922-30723941 | Msa1189280:CDS | 30.0% | |
| TTGTCATAACTTCATCTATG+GGG | + | chr8_1:30723923-30723942 | Msa1189280:CDS | 30.0% | |
| AATCACATGTCCAATATTGG+AGG | + | chr8_1:30723779-30723798 | Msa1189280:CDS | 35.0% | |
| TAGTTGCAGGTACAATACAA+TGG | - | chr8_1:30723900-30723919 | None:intergenic | 35.0% | |
| TATGGGGATCCAAATTTCAA+TGG | + | chr8_1:30723939-30723958 | Msa1189280:CDS | 35.0% | |
| CAAGATCCTCCATTGAAATT+TGG | - | chr8_1:30723951-30723970 | None:intergenic | 35.0% | |
| AGACATATTGAGCCTATCAA+TGG | + | chr8_1:30723988-30724007 | Msa1189280:CDS | 35.0% | |
| ! | CGAAATCGAATAAGAGGTTT+TGG | + | chr8_1:30724034-30724053 | Msa1189280:CDS | 35.0% |
| ! | TTGGATCTTGCTATGACATT+AGG | + | chr8_1:30724090-30724109 | Msa1189280:CDS | 35.0% |
| ! | ATCTTGCTATGACATTAGGT+TGG | + | chr8_1:30724094-30724113 | Msa1189280:CDS | 35.0% |
| CAAGAAGTAGCCTCCAATAT+TGG | - | chr8_1:30723792-30723811 | None:intergenic | 40.0% | |
| TATTGGAGGCTACTTCTTGA+TGG | + | chr8_1:30723793-30723812 | Msa1189280:CDS | 40.0% | |
| ! | GTTATGACAAGACTAGTTGC+AGG | - | chr8_1:30723913-30723932 | None:intergenic | 40.0% |
| ATTGAGCCTATCAATGGTGA+TGG | + | chr8_1:30723994-30724013 | Msa1189280:CDS | 40.0% | |
| ACATCTTCTTCCTTTGCTCA+CGG | - | chr8_1:30724071-30724090 | None:intergenic | 40.0% | |
| TGAGCAAAGGAAGAAGATGT+TGG | + | chr8_1:30724071-30724090 | Msa1189280:CDS | 40.0% | |
| ! | TGTCATGAGTTTTTACCCGC+TGG | + | chr8_1:30723817-30723836 | Msa1189280:CDS | 45.0% |
| GGGGATCCAAATTTCAATGG+AGG | + | chr8_1:30723942-30723961 | Msa1189280:CDS | 45.0% | |
| GGACGTCGAAATCGAATAAG+AGG | + | chr8_1:30724028-30724047 | Msa1189280:CDS | 45.0% | |
| CAAAGTTTACCCGTGAGCAA+AGG | + | chr8_1:30724058-30724077 | Msa1189280:CDS | 45.0% | |
| CATCTTCTTCCTTTGCTCAC+GGG | - | chr8_1:30724070-30724089 | None:intergenic | 45.0% | |
| CCAGCACCATCACCATTGAT+AGG | - | chr8_1:30724003-30724022 | None:intergenic | 50.0% | |
| !! | CCTATCAATGGTGATGGTGC+TGG | + | chr8_1:30724000-30724019 | Msa1189280:CDS | 50.0% |
| !! | AATGGTGATGGTGCTGGAGA+AGG | + | chr8_1:30724006-30724025 | Msa1189280:CDS | 50.0% |
| !! | ATGGTGATGGTGCTGGAGAA+GGG | + | chr8_1:30724007-30724026 | Msa1189280:CDS | 50.0% |
| ! | TTTTTACCCGCTGGAGCCGA+AGG | + | chr8_1:30723826-30723845 | Msa1189280:CDS | 55.0% |
| GTGGTACCTTCGGCTCCAGC+GGG | - | chr8_1:30723835-30723854 | None:intergenic | 65.0% | |
| GGTGGTACCTTCGGCTCCAG+CGG | - | chr8_1:30723836-30723855 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 30723769 | 30724134 | 30723769 | ID=Msa1189280;Name=Msa1189280 |
| chr8_1 | mRNA | 30723769 | 30724134 | 30723769 | ID=Msa1189280-mRNA-1;Parent=Msa1189280;Name=Msa1189280-mRNA-1;_AED=0.02;_eAED=0.12;_QI=0|-1|0|1|-1|1|1|0|121 |
| chr8_1 | exon | 30723769 | 30724134 | 30723769 | ID=Msa1189280-mRNA-1:exon:10641;Parent=Msa1189280-mRNA-1 |
| chr8_1 | CDS | 30723769 | 30724134 | 30723769 | ID=Msa1189280-mRNA-1:cds;Parent=Msa1189280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
