AlfalfaGEDB | Msa1189600

M. sativa cultivar zhongmu-4 / Msa1189600

KEGG: -
GO: GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1189600	sp\|O81906\|B120_ARATH	69.444	72	22	0	1	72	546	617	6.89e-28	112

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1189600	A0A396GKJ2	98.131	107	2	0	73	179	48	154	8.85e-72	223

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1189600	MtrunA17_Chr8g0357781	98.131	107	2	0	73	179	48	154	1.70e-75	223
Msa1189600	MtrunA17_Chr8g0357881	89.720	107	11	0	73	179	693	799	3.70e-62	205
Msa1189600	MtrunA17_Chr8g0357881	95.714	70	3	0	1	70	494	563	1.20e-38	140
Msa1189600	MtrunA17_Chr8g0358141	83.333	108	17	1	72	179	294	400	1.19e-59	191
Msa1189600	MtrunA17_Chr8g0358141	73.529	102	11	1	1	102	84	169	2.35e-42	145
Msa1189600	MtrunA17_Chr8g0357981	86.916	107	13	1	73	179	522	627	2.62e-59	195
Msa1189600	MtrunA17_Chr8g0357981	90.000	70	7	0	1	70	323	392	3.96e-36	132
Msa1189600	MtrunA17_Chr8g0357961	84.906	106	16	0	73	178	701	806	8.64e-59	196
Msa1189600	MtrunA17_Chr8g0357961	92.857	70	5	0	1	70	502	571	1.26e-38	139
Msa1189600	MtrunA17_Chr8g0357991	83.962	106	17	0	73	178	702	807	3.91e-58	194

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1189600	AT3G16030.3	69.444	72	22	0	1	72	565	636	3.60e-29	113
Msa1189600	AT3G16030.8	69.444	72	22	0	1	72	565	636	3.60e-29	113
Msa1189600	AT3G16030.5	69.444	72	22	0	1	72	565	636	3.60e-29	113
Msa1189600	AT4G21390.1	69.444	72	22	0	1	72	546	617	7.00e-29	112
Msa1189600	AT4G21390.1	38.667	75	46	0	74	148	745	819	2.47e-11	62.0
Msa1189600	AT4G21390.2	69.444	72	22	0	1	72	546	617	8.55e-29	112
Msa1189600	AT3G16030.1	68.571	70	22	0	1	70	544	613	8.78e-28	109
Msa1189600	AT3G16030.1	39.189	74	43	2	73	144	744	817	1.53e-11	62.4
Msa1189600	AT3G16030.4	68.571	70	22	0	1	70	565	634	9.21e-28	109
Msa1189600	AT1G67520.1	68.571	70	22	0	1	70	438	507	9.38e-28	108

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Find 32 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACTTTCAAAGACATC+TGG	0.440474	8_1:-31667855	Msa1189600:CDS
AAATGCTGGTCTTCTTGGTA+AGG	0.489595	8_1:+31666597	None:intergenic
AAGGGGAAACAACTTCTAAA+GGG	0.491542	8_1:-31666552	Msa1189600:CDS
AAGTAGAAGGGAAAATATAA+TGG	0.446063	8_1:-31666483	Msa1189600:exon
ACAAATATGTCATTTAATGT+TGG	0.465098	8_1:+31666739	None:intergenic
ACAAATCTTGTCCAACTACT+TGG	0.428393	8_1:-31667777	Msa1189600:CDS
ACAGCAATTTCTTGCCCTGT+TGG	0.495597	8_1:+31667879	None:intergenic
ACATAAAATGCTGGTCTTCT+TGG	0.294568	8_1:+31666592	None:intergenic
AGAAATTGCAGTCATAGAAT+AGG	0.500013	8_1:+31666516	None:intergenic
AGCTTGGATTTCTATCTATT+TGG	0.178416	8_1:-31667699	Msa1189600:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGACTTTCAAAGACATC+TGG	-	chr8_1:31666339-31666358	Msa1189600:three_prime_UTR	30.0%
ATATATGCCAAACAAAAGCT+TGG	-	chr8_1:31666479-31666498	Msa1189600:exon	30.0%
AGCTTGGATTTCTATCTATT+TGG	-	chr8_1:31666495-31666514	Msa1189600:CDS	30.0%
ACACGTCATAAAATACACTT+TGG	+	chr8_1:31666637-31666656	None:intergenic	30.0%
TACGGTGTTTCGAAAATTTA+GGG	-	chr8_1:31666663-31666682	Msa1189600:CDS	30.0%
TCATGTTTCATGTAACTCAA+TGG	-	chr8_1:31666833-31666852	Msa1189600:intron	30.0%
AATCCTGTTGGTATTTACTA+CGG	+	chr8_1:31667067-31667086	None:intergenic	30.0%
TAAATACCAACAGGATTGTT+GGG	-	chr8_1:31667070-31667089	Msa1189600:intron	30.0%
TGGTGAGTATCTACAATTAA+TGG	-	chr8_1:31667430-31667449	Msa1189600:intron	30.0%
GATATAACATCAGACATTGT+AGG	+	chr8_1:31667548-31667567	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	31666306	31667910	31666306	ID=Msa1189600;Name=Msa1189600
chr8_1	mRNA	31666306	31667910	31666306	ID=Msa1189600-mRNA-1;Parent=Msa1189600;Name=Msa1189600-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0\|0\|0.5\|1\|0\|0.5\|2\|180\|179
chr8_1	exon	31666306	31666806	31666306	ID=Msa1189600-mRNA-1:exon:10855;Parent=Msa1189600-mRNA-1
chr8_1	exon	31667692	31667910	31667692	ID=Msa1189600-mRNA-1:exon:10854;Parent=Msa1189600-mRNA-1
chr8_1	CDS	31667692	31667910	31667692	ID=Msa1189600-mRNA-1:cds;Parent=Msa1189600-mRNA-1
chr8_1	CDS	31666486	31666806	31666486	ID=Msa1189600-mRNA-1:cds;Parent=Msa1189600-mRNA-1
chr8_1	three_prime_UTR	31666306	31666485	31666306	ID=Msa1189600-mRNA-1:three_prime_utr;Parent=Msa1189600-mRNA-1

Gene Sequence

>Msa1189600

GGAATTTTGCCAACAGGGCAAGAAATTGCTGTAAAAAGACTTTCAAAGACATCTGGACAAGGAATTGTGGAATTCAAAAATGAATTGGTACTTATATGTGAACTTCAACACACAAATCTTGTCCAACTACTTGGTTGTTGCATTCATCAAGAAGAAAGGATTCTGATTTACGAATATATGCCAAACAAAAGCTTGGATTTCTATCTATTTGGTGAGAATGCATGGGAGCTATGGAATGATGGTGAGTATCTACAATTAATGGATCCAACATTAAATGACATATTTGTTCCAGATGAAGTGCAAAAGTGCATTCATGTTGGTCTCTTATGTGTAGAACAATATGCAAATAATCGACCTACAATGTCTGATGTTATATCAATGTTAACAAACAAATACGCACCGACAACCTTACCAAGAAGACCAGCATTTTATGTTACCAGAGAAATCTTCGAAGGGGAAACAACTTCTAAAGGGCTAGATACTGACACCTATTCTATGACTGCAATTTCTACATCATGTGAAGTAGAAGGGAAAATATAA

Protein sequence

>Msa1189600

GILPTGQEIAVKRLSKTSGQGIVEFKNELVLICELQHTNLVQLLGCCIHQEERILIYEYMPNKSLDFYLFGENAWELWNDGEYLQLMDPTLNDIFVPDEVQKCIHVGLLCVEQYANNRPTMSDVISMLTNKYAPTTLPRRPAFYVTREIFEGETTSKGLDTDTYSMTAISTSCEVEGKI*