AlfalfaGEDB | Msa1190200

M. sativa cultivar zhongmu-4 / Msa1190200

KEGG: K02206
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1190200	KEH19489.1	85.879	347	19	1	1	317	1	347	0.0	608

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1190200	sp\|Q41639\|CDC2_VIGAC	48.136	295	148	2	14	308	1	290	7.40e-95	285

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1190200	MtrunA17_Chr8g0358921	85.630	341	19	1	1	311	1	341	0.0	597
Msa1190200	MtrunA17_Chr4g0051261	47.119	295	151	2	14	308	1	290	3.01e-93	278
Msa1190200	MtrunA17_Chr2g0293491	46.102	295	154	2	14	308	1	290	2.62e-92	276
Msa1190200	MtrunA17_Chr1g0187371	42.715	302	167	4	15	312	16	315	8.26e-83	253
Msa1190200	MtrunA17_Chr4g0002001	41.801	311	162	7	14	309	1	307	3.75e-78	241
Msa1190200	MtrunA17_Chr3g0099951	39.863	291	163	6	21	308	18	299	7.82e-66	212
Msa1190200	MtrunA17_Chr6g0483151	39.860	286	162	5	21	305	18	294	7.79e-64	207
Msa1190200	MtrunA17_Chr4g0057791	36.422	313	177	6	17	312	218	525	1.70e-58	196
Msa1190200	MtrunA17_Chr3g0093571	37.864	309	163	8	17	305	25	324	5.35e-58	194
Msa1190200	MtrunA17_Chr3g0093391	37.908	306	161	8	20	305	26	322	1.44e-57	193

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1190200	AT3G48750.1	45.675	289	152	2	20	308	7	290	4.54e-90	271
Msa1190200	AT1G76540.1	43.686	293	159	4	20	308	17	307	2.04e-82	252
Msa1190200	AT1G20930.1	42.809	299	165	4	14	308	13	309	7.28e-81	248
Msa1190200	AT2G38620.2	41.424	309	166	6	14	309	1	307	4.03e-76	236
Msa1190200	AT3G54180.1	38.762	307	175	5	14	309	1	305	5.67e-71	223
Msa1190200	AT1G18040.1	39.510	286	163	5	21	305	16	292	3.24e-66	213
Msa1190200	AT1G73690.1	38.811	286	165	5	21	305	15	291	5.50e-65	210
Msa1190200	AT1G66750.1	39.437	284	162	5	23	305	19	293	2.57e-64	207
Msa1190200	AT2G38620.4	36.824	296	136	4	14	309	1	245	2.24e-60	193
Msa1190200	AT5G10270.1	37.255	306	163	8	20	305	29	325	1.39e-55	188

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Find 64 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGAGACTTCTCTGATGA+TGG	0.476409	8_1:+33109253	None:intergenic
AACTGGTCTTAATCTTCTAA+TGG	0.406752	8_1:-33107610	Msa1190200:intron
AAGCTTGGTGAAGGTGGTTT+CGG	0.390300	8_1:-33109368	Msa1190200:CDS
AATAATTACCTAACAATGTT+AGG	0.349233	8_1:+33109212	None:intergenic
AATGTTCGGTACCCCAACAG+AGG	0.680862	8_1:-33107830	Msa1190200:CDS
ACATGCTTACTTTGATGACT+TGG	0.444550	8_1:-33106802	Msa1190200:CDS
ACTTCTCTGATGATGGAACC+AGG	0.604779	8_1:+33109260	None:intergenic
AGACCAGTTGGATCAAAAGG+AGG	0.639703	8_1:+33107624	None:intergenic
AGAGTTTAAGAGAGTAGAAT+AGG	0.363500	8_1:+33106768	None:intergenic
AGCATGTCTAAGAGCAGCTT+CGG	0.426384	8_1:+33106818	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAATCATCCTAACATTGTT+AGG	-	chr8_1:33107271-33107290	Msa1190200:intron	30.0%
GTTGAATGTAAAGTACAAGA+AGG	-	chr8_1:33107387-33107406	Msa1190200:intron	30.0%
TAACGATCCAATGACTAAAA+AGG	-	chr8_1:33107498-33107517	Msa1190200:intron	30.0%
TACATATGCACAAATACACA+CGG	+	chr8_1:33107821-33107840	None:intergenic	30.0%
GAAATAGTTATTCACAAGAG+TGG	-	chr8_1:33108204-33108223	Msa1190200:intron	30.0%
ACAAGAGTGGTCAATTATAA+TGG	-	chr8_1:33108217-33108236	Msa1190200:intron	30.0%
TAATTTCACTCACACAAAAC+AGG	-	chr8_1:33108579-33108598	Msa1190200:intron	30.0%
ATTAGAAGATTAAGACCAGT+TGG	+	chr8_1:33108882-33108901	None:intergenic	30.0%
ATTGAAAATGAGACCAAGAA+AGG	+	chr8_1:33109582-33109601	None:intergenic	30.0%
TTCAATACTTTCTTTCATGC+CGG	-	chr8_1:33109596-33109615	Msa1190200:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	33106773	33109740	33106773	ID=Msa1190200;Name=Msa1190200
chr8_1	mRNA	33106773	33109740	33106773	ID=Msa1190200-mRNA-1;Parent=Msa1190200;Name=Msa1190200-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0\|0\|0\|1\|0.85\|0.87\|8\|0\|317
chr8_1	exon	33109710	33109740	33109710	ID=Msa1190200-mRNA-1:exon:11176;Parent=Msa1190200-mRNA-1
chr8_1	exon	33109221	33109422	33109221	ID=Msa1190200-mRNA-1:exon:11175;Parent=Msa1190200-mRNA-1
chr8_1	exon	33108994	33109129	33108994	ID=Msa1190200-mRNA-1:exon:11174;Parent=Msa1190200-mRNA-1
chr8_1	exon	33108736	33108909	33108736	ID=Msa1190200-mRNA-1:exon:11173;Parent=Msa1190200-mRNA-1
chr8_1	exon	33107936	33108099	33107936	ID=Msa1190200-mRNA-1:exon:11172;Parent=Msa1190200-mRNA-1
chr8_1	exon	33107765	33107852	33107765	ID=Msa1190200-mRNA-1:exon:11171;Parent=Msa1190200-mRNA-1
chr8_1	exon	33107611	33107664	33107611	ID=Msa1190200-mRNA-1:exon:11170;Parent=Msa1190200-mRNA-1
chr8_1	exon	33106773	33106877	33106773	ID=Msa1190200-mRNA-1:exon:11169;Parent=Msa1190200-mRNA-1
chr8_1	CDS	33109710	33109740	33109710	ID=Msa1190200-mRNA-1:cds;Parent=Msa1190200-mRNA-1
chr8_1	CDS	33109221	33109422	33109221	ID=Msa1190200-mRNA-1:cds;Parent=Msa1190200-mRNA-1
chr8_1	CDS	33108994	33109129	33108994	ID=Msa1190200-mRNA-1:cds;Parent=Msa1190200-mRNA-1
chr8_1	CDS	33108736	33108909	33108736	ID=Msa1190200-mRNA-1:cds;Parent=Msa1190200-mRNA-1
chr8_1	CDS	33107936	33108099	33107936	ID=Msa1190200-mRNA-1:cds;Parent=Msa1190200-mRNA-1
chr8_1	CDS	33107765	33107852	33107765	ID=Msa1190200-mRNA-1:cds;Parent=Msa1190200-mRNA-1
chr8_1	CDS	33107611	33107664	33107611	ID=Msa1190200-mRNA-1:cds;Parent=Msa1190200-mRNA-1
chr8_1	CDS	33106773	33106877	33106773	ID=Msa1190200-mRNA-1:cds;Parent=Msa1190200-mRNA-1

Gene Sequence

>Msa1190200

ATGCTGAGAAATCTCCAAGAACACGATTCAAGAAAACCGTTCGAAAATCTGGATTTTCTTGAGAAGCTTGGTGAAGGTGGTTTCGGTACAGTTTTCCGTTGCTGTGACGTTGAAACTAAACGAATCGTGGCTGTTAAACAAGTTTCTATTCATGAAACTTATAATGCTGTTCCTGGTTCCATCATCAGAGAAGTCTCGTTTCTTAGGGAATTGAATCATCCTAACATTGTTAGGTTGTTGAATGTAAAGTACAAGAAGGGAACAAGACTCGTGCACTTGGTTTTAGAGTATCTTGATTGTGATCTTCATGATTACATTCTTGACGCGGAACGCTTCGATTCATCTATTAACGATCCAATGACTAAAAAGAGTTTTCTGTATCAGATACTATCTGCGGTGGAGTACTGTCATTCTCGCAAGATTATTCATAGAGATTTGAAACCGAGTAACCTTTTAATTGATCATTCTGAAAAGATAATCAAGTTGGCTGATTTTGGCTTGGCTAGAGAGTTGGGAGATCCTGACGTGTTATATTCGCCGAAGGTAGCAACTCGGTTTTATAGGGCACCTGAGATATTGTTTGAATGTGGCCAATATTCAACTCCAATTGATATATGGTCTGTCGGCTGTATTTTTGGTGAGATGGTACTTGGAATACCAATTCTCCGAGCAATTGATTGCAAGGATGAACTAGAGACGATTTTCAGAATGTTCGGTACCCCAACAGAGGAAACATGGCCAGGAGTCACTGCTTTATGTGCTAACCTTCAAACCTATCCCAAATTTAGACCAATGGACCTGTCATCAATTTTTCCTCCTTTTGATCCAACTGGTCTTAATCTTCTAATGAGGATGCTTTGCTTGGACCCAAACAGGAGGATTTCTGCCGAAGCTGCTCTTAGACATGCTTACTTTGATGACTTGGATCCGGTATATCCTATTCTACTCTCTTAA

Protein sequence

>Msa1190200

MLRNLQEHDSRKPFENLDFLEKLGEGGFGTVFRCCDVETKRIVAVKQVSIHETYNAVPGSIIREVSFLRELNHPNIVRLLNVKYKKGTRLVHLVLEYLDCDLHDYILDAERFDSSINDPMTKKSFLYQILSAVEYCHSRKIIHRDLKPSNLLIDHSEKIIKLADFGLARELGDPDVLYSPKVATRFYRAPEILFECGQYSTPIDIWSVGCIFGEMVLGIPILRAIDCKDELETIFRMFGTPTEETWPGVTALCANLQTYPKFRPMDLSSIFPPFDPTGLNLLMRMLCLDPNRRISAEAALRHAYFDDLDPVYPILLS*