AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1191380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1191380 MtrunA17_Chr8g0349391 99.024 205 2 0 1 205 1 205 1.22e-152 421
Msa1191380 MtrunA17_Chr7g0247111 77.295 207 45 2 1 205 1 207 4.32e-112 318
Msa1191380 MtrunA17_Chr3g0113051 61.497 187 66 2 1 181 1 187 3.31e-83 245
Msa1191380 MtrunA17_Chr8g0386501 54.680 203 86 3 1 202 1 198 4.83e-75 225
Msa1191380 MtrunA17_Chr1g0152431 48.718 195 93 3 1 189 1 194 9.45e-63 193
Msa1191380 MtrunA17_Chr4g0059371 48.045 179 80 2 2 171 3 177 6.27e-57 178
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1191380 AT3G61230.1 69.697 198 56 2 2 195 3 200 1.06e-103 298
Msa1191380 AT1G01780.1 67.500 200 65 0 1 200 1 200 1.87e-101 292
Msa1191380 AT2G45800.1 70.098 204 55 4 1 198 1 204 1.20e-100 291
Msa1191380 AT3G55770.3 67.232 177 54 2 1 173 1 177 1.76e-88 259
Msa1191380 AT3G55770.5 67.232 177 54 2 1 173 1 177 1.76e-88 259
Msa1191380 AT3G55770.2 67.232 177 54 2 1 173 1 177 1.76e-88 259
Msa1191380 AT3G55770.1 67.232 177 54 2 1 173 1 177 1.76e-88 259
Msa1191380 AT2G39900.1 66.292 178 55 2 1 173 1 178 2.46e-87 256
Msa1191380 AT3G55770.7 56.398 211 54 3 1 173 1 211 1.66e-80 240
Msa1191380 AT3G55770.7 42.857 98 54 1 97 194 3 98 2.50e-19 83.2
Msa1191380 AT3G55770.7 35.088 114 40 1 3 82 101 214 2.34e-14 69.7
Msa1191380 AT2G45800.2 65.116 172 51 6 34 198 4 173 1.40e-70 213
Msa1191380 AT2G45800.2 52.113 71 34 0 3 73 68 138 6.49e-21 86.3
Msa1191380 AT1G10200.1 50.286 175 81 2 1 169 1 175 9.84e-61 188
Msa1191380 AT3G55770.4 65.414 133 41 3 1 129 1 132 2.93e-56 175
Msa1191380 AT3G55770.4 42.857 98 54 1 97 194 3 98 1.45e-20 84.0
Msa1191380 AT3G55770.6 66.142 127 37 3 1 121 1 127 1.19e-53 168
Msa1191380 AT3G55770.6 42.857 98 54 1 97 194 3 98 8.83e-21 84.3
Msa1191380 AT1G10200.2 45.385 130 65 2 46 169 4 133 2.32e-37 127
Msa1191380 AT1G10200.2 48.611 72 37 0 3 74 61 132 2.30e-17 75.9

Find 35 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTCAAGAGGTTTCTTC+TGG 0.235129 8_1:-35584907 None:intergenic
GTTTCTTCTGGATGATCTTC+AGG 0.252303 8_1:-35584895 None:intergenic
TCAAGCTATGCTGCTCTTGA+TGG 0.353414 8_1:+35584727 Msa1191380:CDS
CAAAATGAACCCTGCAGTAT+AGG 0.377575 8_1:-35584753 None:intergenic
AGCAAGTCGCACATTTGTCC+AGG 0.436853 8_1:-35584318 None:intergenic
TGCTTTAGGTGTGCTCATGC+AGG 0.437095 8_1:+35584688 Msa1191380:CDS
TTGATGGTGTCCTATACTGC+AGG 0.448405 8_1:+35584743 Msa1191380:CDS
TCTACCACAAGACATGCTTT+AGG 0.453453 8_1:+35584674 Msa1191380:CDS
AACCGTCTATCCACTGGAAA+AGG 0.456725 8_1:+35584345 Msa1191380:CDS
CTCTAAAACCGTCTATCCAC+TGG 0.500295 8_1:+35584339 Msa1191380:CDS
GAAGAAAACATGTCTTTCAC+TGG 0.519623 8_1:+35583276 None:intergenic
TTGTTACTGTTTCATTCAAG+AGG 0.524503 8_1:-35584917 None:intergenic
TTCCATGTTCAGTGGTACCC+TGG 0.525905 8_1:+35584300 Msa1191380:CDS
TCATTGCAAGGGAAATCTTA+CGG 0.534756 8_1:+35583398 Msa1191380:CDS
AACATGGAAGATAGTCTACT+TGG 0.545011 8_1:-35584286 None:intergenic
GGTGCTTCTGCTGATTGTGA+AGG 0.545791 8_1:-35584874 None:intergenic
ATTAAACAGGTGACACTGGA+AGG 0.549370 8_1:+35584646 Msa1191380:intron
CTTCAAATGTAGTCATTGCA+AGG 0.555260 8_1:+35583386 Msa1191380:CDS
GCAATAGAGAATTCCATCCA+TGG 0.558350 8_1:-35583722 None:intergenic
AGTACATACTCCTCCATGGA+TGG 0.562913 8_1:+35583709 Msa1191380:CDS
TTCTGACAAAGCAAATGAGC+TGG 0.565336 8_1:+35583938 Msa1191380:CDS
TACTCAAATCTGCACACAAG+AGG 0.573033 8_1:+35584812 Msa1191380:CDS
GCTTTAGGTGTGCTCATGCA+GGG 0.590942 8_1:+35584689 Msa1191380:CDS
TTCAAATGTAGTCATTGCAA+GGG 0.595286 8_1:+35583387 Msa1191380:CDS
TGATGGTGTCCTATACTGCA+GGG 0.598936 8_1:+35584744 Msa1191380:CDS
GATGAGTACATACTCCTCCA+TGG 0.608526 8_1:+35583705 Msa1191380:intron
GTCCAGGGTACCACTGAACA+TGG 0.612761 8_1:-35584302 None:intergenic
GCATAGCTTGAATGTGTGAG+AGG 0.618734 8_1:-35584715 None:intergenic
AAATCTGCACACAAGAGGAA+TGG 0.618865 8_1:+35584817 Msa1191380:CDS
TGGAACTCTTGATAAATGCA+AGG 0.629692 8_1:+35583296 Msa1191380:CDS
CATAGCTTGAATGTGTGAGA+GGG 0.638300 8_1:-35584714 None:intergenic
AGACTATCTTCCATGTTCAG+TGG 0.654978 8_1:+35584292 Msa1191380:CDS
GCAAGTCGCACATTTGTCCA+GGG 0.661497 8_1:-35584317 None:intergenic
CACACCTAAAGCATGTCTTG+TGG 0.697099 8_1:-35584678 None:intergenic
ATAGAGAATTCCATCCATGG+AGG 0.764020 8_1:-35583719 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATAATTTTTTGTTCATTT+TGG + chr8_1:35583882-35583901 Msa1191380:intron 10.0%
!! TAAAATAAAAATTCAAGTAA+TGG - chr8_1:35584007-35584026 None:intergenic 10.0%
!! AATTTATCTCTAATAATTTA+TGG - chr8_1:35584107-35584126 None:intergenic 10.0%
!!! AATTGTTTCTTTTAAAAATT+TGG + chr8_1:35584432-35584451 Msa1191380:intron 10.0%
!!! ATATAATTTTTTGTTCATTT+TGG + chr8_1:35583882-35583901 Msa1191380:intron 10.0%
!! TAAAATAAAAATTCAAGTAA+TGG - chr8_1:35584007-35584026 None:intergenic 10.0%
!! AATTTATCTCTAATAATTTA+TGG - chr8_1:35584107-35584126 None:intergenic 10.0%
!!! AATTGTTTCTTTTAAAAATT+TGG + chr8_1:35584432-35584451 Msa1191380:intron 10.0%
!!! TTAAAGCTTCAATTTATTTT+TGG + chr8_1:35583626-35583645 Msa1191380:intron 15.0%
!! AAAATAAAAAATGAAGGATT+TGG - chr8_1:35583809-35583828 None:intergenic 15.0%
!! AAAATTATATGTGTATCAAT+TGG - chr8_1:35583872-35583891 None:intergenic 15.0%
!! TAAAAGATAAAGATAAGATA+TGG - chr8_1:35583970-35583989 None:intergenic 15.0%
!!! TTTTTTTGGTTTTATTAAAC+AGG + chr8_1:35584633-35584652 Msa1191380:intron 15.0%
!!! TTAAAGCTTCAATTTATTTT+TGG + chr8_1:35583626-35583645 Msa1191380:intron 15.0%
!! AAAATAAAAAATGAAGGATT+TGG - chr8_1:35583809-35583828 None:intergenic 15.0%
!! AAAATTATATGTGTATCAAT+TGG - chr8_1:35583872-35583891 None:intergenic 15.0%
!! TAAAAGATAAAGATAAGATA+TGG - chr8_1:35583970-35583989 None:intergenic 15.0%
!!! TTTTTTTGGTTTTATTAAAC+AGG + chr8_1:35584633-35584652 Msa1191380:intron 15.0%
!!! TACAGCTTGTTTTAAAATTT+AGG - chr8_1:35583602-35583621 None:intergenic 20.0%
!!! AGACAAATTTCATATCTTTT+TGG - chr8_1:35584075-35584094 None:intergenic 20.0%
!!! GCTTTTTTAAATCAAATTCT+TGG - chr8_1:35584485-35584504 None:intergenic 20.0%
!! AATATGGAATTCATAAATGA+AGG + chr8_1:35584571-35584590 Msa1191380:intron 20.0%
!!! TACAGCTTGTTTTAAAATTT+AGG - chr8_1:35583602-35583621 None:intergenic 20.0%
!!! AGACAAATTTCATATCTTTT+TGG - chr8_1:35584075-35584094 None:intergenic 20.0%
!!! GCTTTTTTAAATCAAATTCT+TGG - chr8_1:35584485-35584504 None:intergenic 20.0%
!! AATATGGAATTCATAAATGA+AGG + chr8_1:35584571-35584590 Msa1191380:intron 20.0%
!!! ATGCTTATTTGAAGCATTTT+AGG + chr8_1:35583534-35583553 Msa1191380:intron 25.0%
! CCAATATTCAATAATGTCAT+TGG - chr8_1:35583681-35583700 None:intergenic 25.0%
! CCAATGACATTATTGAATAT+TGG + chr8_1:35583678-35583697 Msa1191380:intron 25.0%
! AAAAAGTTGAATTAGCCATA+TGG + chr8_1:35584039-35584058 Msa1191380:intron 25.0%
! AATTAGCCATATGGAAATTA+GGG + chr8_1:35584048-35584067 Msa1191380:intron 25.0%
!!! GTATTGATCTTTTACAGAAT+AGG + chr8_1:35584260-35584279 Msa1191380:intron 25.0%
!! AACAATTTTCCACAAAATCA+AGG - chr8_1:35584419-35584438 None:intergenic 25.0%
! TTACCTGATTTCATATAACT+TGG + chr8_1:35584458-35584477 Msa1191380:intron 25.0%
! TTTAAACACAAGCTGTAATA+TGG + chr8_1:35584555-35584574 Msa1191380:intron 25.0%
!! AAAAAATGTGTTACGACTTT+TGG - chr8_1:35584619-35584638 None:intergenic 25.0%
!!! AAAGTCGTAACACATTTTTT+TGG + chr8_1:35584619-35584638 Msa1191380:intron 25.0%
!!! ATGCTTATTTGAAGCATTTT+AGG + chr8_1:35583534-35583553 Msa1191380:intron 25.0%
! CCAATATTCAATAATGTCAT+TGG - chr8_1:35583681-35583700 None:intergenic 25.0%
! CCAATGACATTATTGAATAT+TGG + chr8_1:35583678-35583697 Msa1191380:intron 25.0%
! AAAAAGTTGAATTAGCCATA+TGG + chr8_1:35584039-35584058 Msa1191380:intron 25.0%
! AATTAGCCATATGGAAATTA+GGG + chr8_1:35584048-35584067 Msa1191380:intron 25.0%
!!! GTATTGATCTTTTACAGAAT+AGG + chr8_1:35584260-35584279 Msa1191380:intron 25.0%
!! AACAATTTTCCACAAAATCA+AGG - chr8_1:35584419-35584438 None:intergenic 25.0%
! TTACCTGATTTCATATAACT+TGG + chr8_1:35584458-35584477 Msa1191380:intron 25.0%
! TTTAAACACAAGCTGTAATA+TGG + chr8_1:35584555-35584574 Msa1191380:intron 25.0%
!! AAAAAATGTGTTACGACTTT+TGG - chr8_1:35584619-35584638 None:intergenic 25.0%
!!! AAAGTCGTAACACATTTTTT+TGG + chr8_1:35584619-35584638 Msa1191380:intron 25.0%
!! TACATTTGAAGCAGCTTTTA+TGG - chr8_1:35583377-35583396 None:intergenic 30.0%
TTCAAATGTAGTCATTGCAA+GGG + chr8_1:35583387-35583406 Msa1191380:CDS 30.0%
! AGGAGCATATGAAGAATTTA+AGG - chr8_1:35583461-35583480 None:intergenic 30.0%
ATGTCATTGGTATCTCATTT+AGG - chr8_1:35583668-35583687 None:intergenic 30.0%
! ACATTTTGAACAGCTTTTCA+AGG + chr8_1:35583750-35583769 Msa1191380:CDS 30.0%
GAATTAGCCATATGGAAATT+AGG + chr8_1:35584047-35584066 Msa1191380:intron 30.0%
! TTCATAAATGAAGGTGCATT+CGG + chr8_1:35584580-35584599 Msa1191380:intron 30.0%
! TTTTATTAAACAGGTGACAC+TGG + chr8_1:35584642-35584661 Msa1191380:intron 30.0%
! TCATTTTGCACAACTTTTCA+TGG + chr8_1:35584768-35584787 Msa1191380:CDS 30.0%
!! TACATTTGAAGCAGCTTTTA+TGG - chr8_1:35583377-35583396 None:intergenic 30.0%
TTCAAATGTAGTCATTGCAA+GGG + chr8_1:35583387-35583406 Msa1191380:CDS 30.0%
! AGGAGCATATGAAGAATTTA+AGG - chr8_1:35583461-35583480 None:intergenic 30.0%
ATGTCATTGGTATCTCATTT+AGG - chr8_1:35583668-35583687 None:intergenic 30.0%
! ACATTTTGAACAGCTTTTCA+AGG + chr8_1:35583750-35583769 Msa1191380:CDS 30.0%
GAATTAGCCATATGGAAATT+AGG + chr8_1:35584047-35584066 Msa1191380:intron 30.0%
! TTCATAAATGAAGGTGCATT+CGG + chr8_1:35584580-35584599 Msa1191380:intron 30.0%
! TTTTATTAAACAGGTGACAC+TGG + chr8_1:35584642-35584661 Msa1191380:intron 30.0%
! TCATTTTGCACAACTTTTCA+TGG + chr8_1:35584768-35584787 Msa1191380:CDS 30.0%
TGGAACTCTTGATAAATGCA+AGG + chr8_1:35583296-35583315 Msa1191380:CDS 35.0%
! TTGAAGCAGCTTTTATGGTA+TGG - chr8_1:35583372-35583391 None:intergenic 35.0%
CTTCAAATGTAGTCATTGCA+AGG + chr8_1:35583386-35583405 Msa1191380:CDS 35.0%
TCATTGCAAGGGAAATCTTA+CGG + chr8_1:35583398-35583417 Msa1191380:CDS 35.0%
!! GGCAATTTTAGCAAGAACTT+TGG + chr8_1:35583778-35583797 Msa1191380:CDS 35.0%
TTTGGTCCCTAATTTCCATA+TGG - chr8_1:35584057-35584076 None:intergenic 35.0%
AACATGGAAGATAGTCTACT+TGG - chr8_1:35584289-35584308 None:intergenic 35.0%
! TGTTGTGATCCTTGATTTTG+TGG + chr8_1:35584407-35584426 Msa1191380:intron 35.0%
GGTCCAAGTTATATGAAATC+AGG - chr8_1:35584464-35584483 None:intergenic 35.0%
AAAGCAATCATACGTTTCGT+TGG + chr8_1:35584500-35584519 Msa1191380:intron 35.0%
ATCATACGTTTCGTTGGATA+TGG + chr8_1:35584506-35584525 Msa1191380:intron 35.0%
TGGAACTCTTGATAAATGCA+AGG + chr8_1:35583296-35583315 Msa1191380:CDS 35.0%
! TTGAAGCAGCTTTTATGGTA+TGG - chr8_1:35583372-35583391 None:intergenic 35.0%
CTTCAAATGTAGTCATTGCA+AGG + chr8_1:35583386-35583405 Msa1191380:CDS 35.0%
TCATTGCAAGGGAAATCTTA+CGG + chr8_1:35583398-35583417 Msa1191380:CDS 35.0%
!! GGCAATTTTAGCAAGAACTT+TGG + chr8_1:35583778-35583797 Msa1191380:CDS 35.0%
TTTGGTCCCTAATTTCCATA+TGG - chr8_1:35584057-35584076 None:intergenic 35.0%
AACATGGAAGATAGTCTACT+TGG - chr8_1:35584289-35584308 None:intergenic 35.0%
! TGTTGTGATCCTTGATTTTG+TGG + chr8_1:35584407-35584426 Msa1191380:intron 35.0%
GGTCCAAGTTATATGAAATC+AGG - chr8_1:35584464-35584483 None:intergenic 35.0%
AAAGCAATCATACGTTTCGT+TGG + chr8_1:35584500-35584519 Msa1191380:intron 35.0%
ATCATACGTTTCGTTGGATA+TGG + chr8_1:35584506-35584525 Msa1191380:intron 35.0%
ATAGAGAATTCCATCCATGG+AGG - chr8_1:35583722-35583741 None:intergenic 40.0%
GCAATAGAGAATTCCATCCA+TGG - chr8_1:35583725-35583744 None:intergenic 40.0%
!! GAACAGCTTTTCAAGGAATC+TGG + chr8_1:35583757-35583776 Msa1191380:CDS 40.0%
TTCTGACAAAGCAAATGAGC+TGG + chr8_1:35583938-35583957 Msa1191380:CDS 40.0%
AGACTATCTTCCATGTTCAG+TGG + chr8_1:35584292-35584311 Msa1191380:CDS 40.0%
! AACCTTTTCCAGTGGATAGA+CGG - chr8_1:35584350-35584369 None:intergenic 40.0%
! TGTCGCGATTCTTGATTTTG+AGG + chr8_1:35584370-35584389 Msa1191380:intron 40.0%
! TGTGTTACGACTTTTGGTCA+CGG - chr8_1:35584613-35584632 None:intergenic 40.0%
ATTAAACAGGTGACACTGGA+AGG + chr8_1:35584646-35584665 Msa1191380:intron 40.0%
TCTACCACAAGACATGCTTT+AGG + chr8_1:35584674-35584693 Msa1191380:CDS 40.0%
CATAGCTTGAATGTGTGAGA+GGG - chr8_1:35584717-35584736 None:intergenic 40.0%
CAAAATGAACCCTGCAGTAT+AGG - chr8_1:35584756-35584775 None:intergenic 40.0%
! GCACAACTTTTCATGGAGAA+AGG + chr8_1:35584775-35584794 Msa1191380:CDS 40.0%
TACTCAAATCTGCACACAAG+AGG + chr8_1:35584812-35584831 Msa1191380:CDS 40.0%
AAATCTGCACACAAGAGGAA+TGG + chr8_1:35584817-35584836 Msa1191380:CDS 40.0%
GTTTCTTCTGGATGATCTTC+AGG - chr8_1:35584898-35584917 None:intergenic 40.0%
ATAGAGAATTCCATCCATGG+AGG - chr8_1:35583722-35583741 None:intergenic 40.0%
GCAATAGAGAATTCCATCCA+TGG - chr8_1:35583725-35583744 None:intergenic 40.0%
!! GAACAGCTTTTCAAGGAATC+TGG + chr8_1:35583757-35583776 Msa1191380:CDS 40.0%
TTCTGACAAAGCAAATGAGC+TGG + chr8_1:35583938-35583957 Msa1191380:CDS 40.0%
AGACTATCTTCCATGTTCAG+TGG + chr8_1:35584292-35584311 Msa1191380:CDS 40.0%
! AACCTTTTCCAGTGGATAGA+CGG - chr8_1:35584350-35584369 None:intergenic 40.0%
! TGTCGCGATTCTTGATTTTG+AGG + chr8_1:35584370-35584389 Msa1191380:intron 40.0%
! TGTGTTACGACTTTTGGTCA+CGG - chr8_1:35584613-35584632 None:intergenic 40.0%
ATTAAACAGGTGACACTGGA+AGG + chr8_1:35584646-35584665 Msa1191380:intron 40.0%
TCTACCACAAGACATGCTTT+AGG + chr8_1:35584674-35584693 Msa1191380:CDS 40.0%
CATAGCTTGAATGTGTGAGA+GGG - chr8_1:35584717-35584736 None:intergenic 40.0%
CAAAATGAACCCTGCAGTAT+AGG - chr8_1:35584756-35584775 None:intergenic 40.0%
! GCACAACTTTTCATGGAGAA+AGG + chr8_1:35584775-35584794 Msa1191380:CDS 40.0%
TACTCAAATCTGCACACAAG+AGG + chr8_1:35584812-35584831 Msa1191380:CDS 40.0%
AAATCTGCACACAAGAGGAA+TGG + chr8_1:35584817-35584836 Msa1191380:CDS 40.0%
GTTTCTTCTGGATGATCTTC+AGG - chr8_1:35584898-35584917 None:intergenic 40.0%
! TGGAGGAGTATGTACTCATC+TGG - chr8_1:35583705-35583724 None:intergenic 45.0%
GATGAGTACATACTCCTCCA+TGG + chr8_1:35583705-35583724 Msa1191380:intron 45.0%
AGTACATACTCCTCCATGGA+TGG + chr8_1:35583709-35583728 Msa1191380:CDS 45.0%
!! CTCTAAAACCGTCTATCCAC+TGG + chr8_1:35584339-35584358 Msa1191380:CDS 45.0%
AACCGTCTATCCACTGGAAA+AGG + chr8_1:35584345-35584364 Msa1191380:CDS 45.0%
CACACCTAAAGCATGTCTTG+TGG - chr8_1:35584681-35584700 None:intergenic 45.0%
GCATAGCTTGAATGTGTGAG+AGG - chr8_1:35584718-35584737 None:intergenic 45.0%
TCAAGCTATGCTGCTCTTGA+TGG + chr8_1:35584727-35584746 Msa1191380:CDS 45.0%
!! TTGATGGTGTCCTATACTGC+AGG + chr8_1:35584743-35584762 Msa1191380:CDS 45.0%
! TGATGGTGTCCTATACTGCA+GGG + chr8_1:35584744-35584763 Msa1191380:CDS 45.0%
! TGGAGGAGTATGTACTCATC+TGG - chr8_1:35583705-35583724 None:intergenic 45.0%
GATGAGTACATACTCCTCCA+TGG + chr8_1:35583705-35583724 Msa1191380:intron 45.0%
AGTACATACTCCTCCATGGA+TGG + chr8_1:35583709-35583728 Msa1191380:CDS 45.0%
!! CTCTAAAACCGTCTATCCAC+TGG + chr8_1:35584339-35584358 Msa1191380:CDS 45.0%
AACCGTCTATCCACTGGAAA+AGG + chr8_1:35584345-35584364 Msa1191380:CDS 45.0%
CACACCTAAAGCATGTCTTG+TGG - chr8_1:35584681-35584700 None:intergenic 45.0%
GCATAGCTTGAATGTGTGAG+AGG - chr8_1:35584718-35584737 None:intergenic 45.0%
TCAAGCTATGCTGCTCTTGA+TGG + chr8_1:35584727-35584746 Msa1191380:CDS 45.0%
!! TTGATGGTGTCCTATACTGC+AGG + chr8_1:35584743-35584762 Msa1191380:CDS 45.0%
! TGATGGTGTCCTATACTGCA+GGG + chr8_1:35584744-35584763 Msa1191380:CDS 45.0%
!! TATGTATATAAATAATTAAA+AGG - chr8_1:35583481-35583500 None:intergenic 5.0%
!! AAAATTAAAATAAAAAATGA+AGG - chr8_1:35583815-35583834 None:intergenic 5.0%
!! TATGTATATAAATAATTAAA+AGG - chr8_1:35583481-35583500 None:intergenic 5.0%
!! AAAATTAAAATAAAAAATGA+AGG - chr8_1:35583815-35583834 None:intergenic 5.0%
TTCCATGTTCAGTGGTACCC+TGG + chr8_1:35584300-35584319 Msa1191380:CDS 50.0%
GCAAGTCGCACATTTGTCCA+GGG - chr8_1:35584320-35584339 None:intergenic 50.0%
AGCAAGTCGCACATTTGTCC+AGG - chr8_1:35584321-35584340 None:intergenic 50.0%
! TCGCGACAAACCTTTTCCAG+TGG - chr8_1:35584358-35584377 None:intergenic 50.0%
! CGACTTTTGGTCACGGAGTT+TGG - chr8_1:35584606-35584625 None:intergenic 50.0%
! TGCTTTAGGTGTGCTCATGC+AGG + chr8_1:35584688-35584707 Msa1191380:CDS 50.0%
! GCTTTAGGTGTGCTCATGCA+GGG + chr8_1:35584689-35584708 Msa1191380:CDS 50.0%
! GGTGCTTCTGCTGATTGTGA+AGG - chr8_1:35584877-35584896 None:intergenic 50.0%
TTCCATGTTCAGTGGTACCC+TGG + chr8_1:35584300-35584319 Msa1191380:CDS 50.0%
GCAAGTCGCACATTTGTCCA+GGG - chr8_1:35584320-35584339 None:intergenic 50.0%
AGCAAGTCGCACATTTGTCC+AGG - chr8_1:35584321-35584340 None:intergenic 50.0%
! TCGCGACAAACCTTTTCCAG+TGG - chr8_1:35584358-35584377 None:intergenic 50.0%
! CGACTTTTGGTCACGGAGTT+TGG - chr8_1:35584606-35584625 None:intergenic 50.0%
! TGCTTTAGGTGTGCTCATGC+AGG + chr8_1:35584688-35584707 Msa1191380:CDS 50.0%
! GCTTTAGGTGTGCTCATGCA+GGG + chr8_1:35584689-35584708 Msa1191380:CDS 50.0%
! GGTGCTTCTGCTGATTGTGA+AGG - chr8_1:35584877-35584896 None:intergenic 50.0%
! GTCCAGGGTACCACTGAACA+TGG - chr8_1:35584305-35584324 None:intergenic 55.0%
! GTCCAGGGTACCACTGAACA+TGG - chr8_1:35584305-35584324 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 35583285 35584924 35583285 ID=Msa1191380;Name=Msa1191380
chr8_1 mRNA 35583285 35584924 35583285 ID=Msa1191380-mRNA-1;Parent=Msa1191380;Name=Msa1191380-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|1|1|5|0|205
chr8_1 exon 35583285 35583419 35583285 ID=Msa1191380-mRNA-1:exon:11761;Parent=Msa1191380-mRNA-1
chr8_1 exon 35583706 35583799 35583706 ID=Msa1191380-mRNA-1:exon:11762;Parent=Msa1191380-mRNA-1
chr8_1 exon 35583931 35583959 35583931 ID=Msa1191380-mRNA-1:exon:11763;Parent=Msa1191380-mRNA-1
chr8_1 exon 35584277 35584366 35584277 ID=Msa1191380-mRNA-1:exon:11764;Parent=Msa1191380-mRNA-1
chr8_1 exon 35584655 35584924 35584655 ID=Msa1191380-mRNA-1:exon:11765;Parent=Msa1191380-mRNA-1
chr8_1 CDS 35583285 35583419 35583285 ID=Msa1191380-mRNA-1:cds;Parent=Msa1191380-mRNA-1
chr8_1 CDS 35583706 35583799 35583706 ID=Msa1191380-mRNA-1:cds;Parent=Msa1191380-mRNA-1
chr8_1 CDS 35583931 35583959 35583931 ID=Msa1191380-mRNA-1:cds;Parent=Msa1191380-mRNA-1
chr8_1 CDS 35584277 35584366 35584277 ID=Msa1191380-mRNA-1:cds;Parent=Msa1191380-mRNA-1
chr8_1 CDS 35584655 35584924 35584655 ID=Msa1191380-mRNA-1:cds;Parent=Msa1191380-mRNA-1
Gene Sequence

>Msa1191380

ATGTCTTTCACTGGAACTCTTGATAAATGCAAGGCTTGTGACAAGACTGTTTATGTTGTTGATTTGTTAACTCTTGAAAATATTCCATACCATAAAAGCTGCTTCAAATGTAGTCATTGCAAGGGAAATCTTACGATGAGTACATACTCCTCCATGGATGGAATTCTCTATTGCAAAACACATTTTGAACAGCTTTTCAAGGAATCTGGCAATTTTAGCAAGAACTTTGCAAAGTCTTCTGACAAAGCAAATGAGCTGAATAGGACACCAAGTAGACTATCTTCCATGTTCAGTGGTACCCTGGACAAATGTGCGACTTGCTCTAAAACCGTCTATCCACTGGAAAAGGTGACACTGGAAGGAGAAATCTACCACAAGACATGCTTTAGGTGTGCTCATGCAGGGTGCCCTCTCACACATTCAAGCTATGCTGCTCTTGATGGTGTCCTATACTGCAGGGTTCATTTTGCACAACTTTTCATGGAGAAAGGTAACTACAGTCATGTACTCAAATCTGCACACAAGAGGAATGGTTCTTCTTCTGAATCAGTTGAAGTTGTTGAAGAGCCTTCACAATCAGCAGAAGCACCTGAAGATCATCCAGAAGAAACCTCTTGA
Protein sequence

>Msa1191380

MSFTGTLDKCKACDKTVYVVDLLTLENIPYHKSCFKCSHCKGNLTMSTYSSMDGILYCKTHFEQLFKESGNFSKNFAKSSDKANELNRTPSRLSSMFSGTLDKCATCSKTVYPLEKVTLEGEIYHKTCFRCAHAGCPLTHSSYAALDGVLYCRVHFAQLFMEKGNYSHVLKSAHKRNGSSSESVEVVEEPSQSAEAPEDHPEETS*