Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1193960 | sp|F4JZT3|ARR24_ARATH | 55.932 | 118 | 50 | 1 | 10 | 127 | 16 | 131 | 1.53e-35 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1193960 | G7LG58 | 93.056 | 144 | 10 | 0 | 1 | 144 | 1 | 144 | 6.54e-93 | 274 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1193960 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1193960 | MtrunA17_Chr8g0364781 | 93.056 | 144 | 10 | 0 | 1 | 144 | 1 | 144 | 1.26e-96 | 274 |
| Msa1193960 | MtrunA17_Chr7g0262351 | 57.600 | 125 | 50 | 1 | 5 | 129 | 15 | 136 | 1.26e-41 | 135 |
| Msa1193960 | MtrunA17_Chr7g0262671 | 62.821 | 78 | 28 | 1 | 1 | 78 | 1 | 77 | 2.08e-27 | 97.8 |
| Msa1193960 | MtrunA17_Chr6g0472261 | 54.795 | 73 | 33 | 0 | 6 | 78 | 4 | 76 | 7.98e-20 | 77.8 |
| Msa1193960 | MtrunA17_Chr7g0262681 | 64.179 | 67 | 19 | 2 | 66 | 132 | 1 | 62 | 1.18e-18 | 74.7 |
| Msa1193960 | MtrunA17_Chr8g0371601 | 31.206 | 141 | 74 | 4 | 12 | 131 | 1029 | 1167 | 2.02e-13 | 66.2 |
| Msa1193960 | MtrunA17_Chr8g0392301 | 31.159 | 138 | 67 | 4 | 15 | 132 | 864 | 993 | 2.97e-13 | 65.9 |
| Msa1193960 | MtrunA17_Chr6g0451941 | 33.333 | 108 | 70 | 1 | 12 | 119 | 33 | 138 | 6.32e-13 | 62.0 |
| Msa1193960 | MtrunA17_Chr5g0447641 | 41.791 | 67 | 39 | 0 | 15 | 81 | 1077 | 1143 | 2.57e-11 | 60.5 |
| Msa1193960 | MtrunA17_Chr8g0377481 | 32.759 | 116 | 70 | 2 | 15 | 122 | 844 | 959 | 2.62e-11 | 60.1 |
| Msa1193960 | MtrunA17_Chr1g0194731 | 37.190 | 121 | 56 | 5 | 15 | 122 | 331 | 444 | 6.96e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1193960 | AT5G26594.1 | 55.932 | 118 | 50 | 1 | 10 | 127 | 16 | 131 | 1.56e-36 | 122 |
| Msa1193960 | AT3G04280.1 | 51.786 | 112 | 52 | 2 | 12 | 122 | 22 | 132 | 6.27e-32 | 111 |
| Msa1193960 | AT3G04280.2 | 51.786 | 112 | 52 | 2 | 12 | 122 | 22 | 132 | 6.27e-32 | 111 |
| Msa1193960 | AT3G04280.3 | 51.786 | 112 | 52 | 2 | 12 | 122 | 22 | 132 | 6.27e-32 | 111 |
| Msa1193960 | AT2G17820.1 | 27.950 | 161 | 78 | 4 | 15 | 139 | 1047 | 1205 | 2.94e-13 | 66.2 |
| Msa1193960 | AT2G01830.5 | 30.579 | 121 | 66 | 3 | 15 | 122 | 937 | 1052 | 4.87e-12 | 62.8 |
| Msa1193960 | AT2G01830.2 | 30.579 | 121 | 66 | 3 | 15 | 122 | 948 | 1063 | 5.21e-12 | 62.8 |
| Msa1193960 | AT2G01830.1 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.25e-12 | 62.8 |
| Msa1193960 | AT2G01830.4 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.25e-12 | 62.8 |
| Msa1193960 | AT2G01830.6 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.25e-12 | 62.8 |
| Msa1193960 | AT2G01830.3 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.25e-12 | 62.8 |
| Msa1193960 | AT2G47430.1 | 33.333 | 123 | 62 | 5 | 15 | 119 | 989 | 1109 | 1.38e-11 | 61.6 |
Find 25 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAATAGTGATAAAATTTA+TGG | 0.245050 | 8_1:+40936537 | Msa1193960:CDS |
| TGGTGTATCCTCACACTCTA+TGG | 0.342570 | 8_1:+40936507 | Msa1193960:CDS |
| TGACCTAATCTTGATGGATA+AGG | 0.347722 | 8_1:+40936230 | Msa1193960:CDS |
| ACGTCATTTGATGAATTCTT+CGG | 0.351042 | 8_1:-40936128 | None:intergenic |
| CACAACAAGTGCTGTTAACT+TGG | 0.355344 | 8_1:-40936088 | None:intergenic |
| CGTCATTTGATGAATTCTTC+GGG | 0.366651 | 8_1:-40936127 | None:intergenic |
| GTGATAAAATTTATGGAAGC+AGG | 0.456780 | 8_1:+40936544 | Msa1193960:CDS |
| AATTCCATTCTTCATGCCGT+TGG | 0.460947 | 8_1:+40936610 | Msa1193960:CDS |
| TATCCTTATCCATCAAGATT+AGG | 0.462938 | 8_1:-40936233 | None:intergenic |
| TCTTGAGAAACCATTGGATA+AGG | 0.467024 | 8_1:+40936579 | Msa1193960:CDS |
| AGGATATCTAGCACTATTGT+TGG | 0.469888 | 8_1:+40936487 | Msa1193960:CDS |
| TCATTTCATCTTGTGAGGAA+TGG | 0.470437 | 8_1:-40936656 | None:intergenic |
| TATAGGCAACAAAGCAACTA+AGG | 0.474253 | 8_1:+40936458 | Msa1193960:intron |
| CAAATGACGTTGAGTAACTT+AGG | 0.479404 | 8_1:+40936141 | Msa1193960:CDS |
| ACCTCAGCTCCATTCATCTC+AGG | 0.491878 | 8_1:-40936258 | None:intergenic |
| AAGGATATGCCTGAGATGAA+TGG | 0.499108 | 8_1:+40936249 | Msa1193960:CDS |
| CGAATATCTTGAGAAACCAT+TGG | 0.533361 | 8_1:+40936573 | Msa1193960:CDS |
| TTTGTTCATTTCATCTTGTG+AGG | 0.533712 | 8_1:-40936661 | None:intergenic |
| CTCCTCTTCCATAGAGTGTG+AGG | 0.555361 | 8_1:-40936515 | None:intergenic |
| ATCCTCACACTCTATGGAAG+AGG | 0.570487 | 8_1:+40936513 | Msa1193960:CDS |
| CAAAGCAACTAAGGTTGATG+AGG | 0.578109 | 8_1:+40936467 | Msa1193960:CDS |
| CAAGTTAACAGCACTTGTTG+TGG | 0.584291 | 8_1:+40936089 | Msa1193960:CDS |
| CTCACACTCTATGGAAGAGG+AGG | 0.604784 | 8_1:+40936516 | Msa1193960:CDS |
| GCAGTGGAAATTCATCAGCA+TGG | 0.649906 | 8_1:+40936198 | Msa1193960:CDS |
| GCCTGAGATGAATGGAGCTG+AGG | 0.680215 | 8_1:+40936257 | Msa1193960:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATGTGTTTCTTATAT+AGG | + | chr8_1:40936441-40936460 | Msa1193960:intron | 15.0% |
| !!! | TTTTTCTTTTATTTTTCCAA+CGG | - | chr8_1:40936629-40936648 | None:intergenic | 15.0% |
| !! | GGAAATAGTGATAAAATTTA+TGG | + | chr8_1:40936537-40936556 | Msa1193960:CDS | 20.0% |
| !!! | ATTTTTTGACCTAATCTTGA+TGG | + | chr8_1:40936224-40936243 | Msa1193960:CDS | 25.0% |
| !!! | TTGGATGTAAAGTTTTGAGA+TGG | - | chr8_1:40936072-40936091 | None:intergenic | 30.0% |
| ACGTCATTTGATGAATTCTT+CGG | - | chr8_1:40936131-40936150 | None:intergenic | 30.0% | |
| AACAACACTTGTGTGAAAAA+CGG | + | chr8_1:40936168-40936187 | Msa1193960:CDS | 30.0% | |
| TATCCTTATCCATCAAGATT+AGG | - | chr8_1:40936236-40936255 | None:intergenic | 30.0% | |
| GAGTTCATTCTTGCTTATTT+AGG | + | chr8_1:40936339-40936358 | Msa1193960:intron | 30.0% | |
| GTGATAAAATTTATGGAAGC+AGG | + | chr8_1:40936544-40936563 | Msa1193960:CDS | 30.0% | |
| CGTCATTTGATGAATTCTTC+GGG | - | chr8_1:40936130-40936149 | None:intergenic | 35.0% | |
| CAAATGACGTTGAGTAACTT+AGG | + | chr8_1:40936141-40936160 | Msa1193960:CDS | 35.0% | |
| ! | TGACCTAATCTTGATGGATA+AGG | + | chr8_1:40936230-40936249 | Msa1193960:CDS | 35.0% |
| TATAGGCAACAAAGCAACTA+AGG | + | chr8_1:40936458-40936477 | Msa1193960:intron | 35.0% | |
| AGGATATCTAGCACTATTGT+TGG | + | chr8_1:40936487-40936506 | Msa1193960:CDS | 35.0% | |
| CGAATATCTTGAGAAACCAT+TGG | + | chr8_1:40936573-40936592 | Msa1193960:CDS | 35.0% | |
| TCTTGAGAAACCATTGGATA+AGG | + | chr8_1:40936579-40936598 | Msa1193960:CDS | 35.0% | |
| ! | TTGAGTTTTGCCTTATCCAA+TGG | - | chr8_1:40936592-40936611 | None:intergenic | 35.0% |
| TCATTTCATCTTGTGAGGAA+TGG | - | chr8_1:40936659-40936678 | None:intergenic | 35.0% | |
| ! | CACAACAAGTGCTGTTAACT+TGG | - | chr8_1:40936091-40936110 | None:intergenic | 40.0% |
| ! | CAAGTTAACAGCACTTGTTG+TGG | + | chr8_1:40936089-40936108 | Msa1193960:CDS | 40.0% |
| GAAAAACGGAAAAGAAGCAG+TGG | + | chr8_1:40936182-40936201 | Msa1193960:CDS | 40.0% | |
| AAGGATATGCCTGAGATGAA+TGG | + | chr8_1:40936249-40936268 | Msa1193960:CDS | 40.0% | |
| CATTCTTGCTTATTTAGGCG+TGG | + | chr8_1:40936344-40936363 | Msa1193960:intron | 40.0% | |
| !! | CAAAGCAACTAAGGTTGATG+AGG | + | chr8_1:40936467-40936486 | Msa1193960:CDS | 40.0% |
| AATTCCATTCTTCATGCCGT+TGG | + | chr8_1:40936610-40936629 | Msa1193960:CDS | 40.0% | |
| ! | TTTTCCAACGGCATGAAGAA+TGG | - | chr8_1:40936617-40936636 | None:intergenic | 40.0% |
| GCAGTGGAAATTCATCAGCA+TGG | + | chr8_1:40936198-40936217 | Msa1193960:CDS | 45.0% | |
| TGGTGTATCCTCACACTCTA+TGG | + | chr8_1:40936507-40936526 | Msa1193960:CDS | 45.0% | |
| ATCCTCACACTCTATGGAAG+AGG | + | chr8_1:40936513-40936532 | Msa1193960:CDS | 45.0% | |
| !! | ATATATAAATGTAAAATTTA+GGG | - | chr8_1:40936303-40936322 | None:intergenic | 5.0% |
| !! | TATATATAAATGTAAAATTT+AGG | - | chr8_1:40936304-40936323 | None:intergenic | 5.0% |
| ACCTCAGCTCCATTCATCTC+AGG | - | chr8_1:40936261-40936280 | None:intergenic | 50.0% | |
| CTCCTCTTCCATAGAGTGTG+AGG | - | chr8_1:40936518-40936537 | None:intergenic | 50.0% | |
| CTCACACTCTATGGAAGAGG+AGG | + | chr8_1:40936516-40936535 | Msa1193960:CDS | 50.0% | |
| GCCTGAGATGAATGGAGCTG+AGG | + | chr8_1:40936257-40936276 | Msa1193960:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 40936060 | 40936678 | 40936060 | ID=Msa1193960;Name=Msa1193960 |
| chr8_1 | mRNA | 40936060 | 40936678 | 40936060 | ID=Msa1193960-mRNA-1;Parent=Msa1193960;Name=Msa1193960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|0|0|1|1|1|2|0|144 |
| chr8_1 | exon | 40936060 | 40936278 | 40936060 | ID=Msa1193960-mRNA-1:exon:13208;Parent=Msa1193960-mRNA-1 |
| chr8_1 | exon | 40936463 | 40936678 | 40936463 | ID=Msa1193960-mRNA-1:exon:13209;Parent=Msa1193960-mRNA-1 |
| chr8_1 | CDS | 40936060 | 40936278 | 40936060 | ID=Msa1193960-mRNA-1:cds;Parent=Msa1193960-mRNA-1 |
| chr8_1 | CDS | 40936463 | 40936678 | 40936463 | ID=Msa1193960-mRNA-1:cds;Parent=Msa1193960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
