AlfalfaGEDB | Msa1193960

M. sativa cultivar zhongmu-4 / Msa1193960

KEGG: -
GO: -
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1193960	sp\|F4JZT3\|ARR24_ARATH	55.932	118	50	1	10	127	16	131	1.53e-35	122

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1193960	G7LG58	93.056	144	10	0	1	144	1	144	6.54e-93	274

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TFs/TRs:

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Gene ID	Type	Classification
Msa1193960	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1193960	MtrunA17_Chr8g0364781	93.056	144	10	0	1	144	1	144	1.26e-96	274
Msa1193960	MtrunA17_Chr7g0262351	57.600	125	50	1	5	129	15	136	1.26e-41	135
Msa1193960	MtrunA17_Chr7g0262671	62.821	78	28	1	1	78	1	77	2.08e-27	97.8
Msa1193960	MtrunA17_Chr6g0472261	54.795	73	33	0	6	78	4	76	7.98e-20	77.8
Msa1193960	MtrunA17_Chr7g0262681	64.179	67	19	2	66	132	1	62	1.18e-18	74.7
Msa1193960	MtrunA17_Chr8g0371601	31.206	141	74	4	12	131	1029	1167	2.02e-13	66.2
Msa1193960	MtrunA17_Chr8g0392301	31.159	138	67	4	15	132	864	993	2.97e-13	65.9
Msa1193960	MtrunA17_Chr6g0451941	33.333	108	70	1	12	119	33	138	6.32e-13	62.0
Msa1193960	MtrunA17_Chr5g0447641	41.791	67	39	0	15	81	1077	1143	2.57e-11	60.5
Msa1193960	MtrunA17_Chr8g0377481	32.759	116	70	2	15	122	844	959	2.62e-11	60.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1193960	AT5G26594.1	55.932	118	50	1	10	127	16	131	1.56e-36	122
Msa1193960	AT3G04280.1	51.786	112	52	2	12	122	22	132	6.27e-32	111
Msa1193960	AT3G04280.2	51.786	112	52	2	12	122	22	132	6.27e-32	111
Msa1193960	AT3G04280.3	51.786	112	52	2	12	122	22	132	6.27e-32	111
Msa1193960	AT2G17820.1	27.950	161	78	4	15	139	1047	1205	2.94e-13	66.2
Msa1193960	AT2G01830.5	30.579	121	66	3	15	122	937	1052	4.87e-12	62.8
Msa1193960	AT2G01830.2	30.579	121	66	3	15	122	948	1063	5.21e-12	62.8
Msa1193960	AT2G01830.1	30.579	121	66	3	15	122	925	1040	5.25e-12	62.8
Msa1193960	AT2G01830.4	30.579	121	66	3	15	122	925	1040	5.25e-12	62.8
Msa1193960	AT2G01830.6	30.579	121	66	3	15	122	925	1040	5.25e-12	62.8

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Find 25 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGATATGCCTGAGATGAA+TGG	0.499108	8_1:+40936249	Msa1193960:CDS
AATTCCATTCTTCATGCCGT+TGG	0.460947	8_1:+40936610	Msa1193960:CDS
ACCTCAGCTCCATTCATCTC+AGG	0.491878	8_1:-40936258	None:intergenic
ACGTCATTTGATGAATTCTT+CGG	0.351042	8_1:-40936128	None:intergenic
AGGATATCTAGCACTATTGT+TGG	0.469888	8_1:+40936487	Msa1193960:CDS
ATCCTCACACTCTATGGAAG+AGG	0.570487	8_1:+40936513	Msa1193960:CDS
CAAAGCAACTAAGGTTGATG+AGG	0.578109	8_1:+40936467	Msa1193960:CDS
CAAATGACGTTGAGTAACTT+AGG	0.479404	8_1:+40936141	Msa1193960:CDS
CAAGTTAACAGCACTTGTTG+TGG	0.584291	8_1:+40936089	Msa1193960:CDS
CACAACAAGTGCTGTTAACT+TGG	0.355344	8_1:-40936088	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACGTCATTTGATGAATTCTT+CGG	-	chr8_1:40936131-40936150	None:intergenic	30.0%
AACAACACTTGTGTGAAAAA+CGG	+	chr8_1:40936168-40936187	Msa1193960:CDS	30.0%
TATCCTTATCCATCAAGATT+AGG	-	chr8_1:40936236-40936255	None:intergenic	30.0%
GAGTTCATTCTTGCTTATTT+AGG	+	chr8_1:40936339-40936358	Msa1193960:intron	30.0%
GTGATAAAATTTATGGAAGC+AGG	+	chr8_1:40936544-40936563	Msa1193960:CDS	30.0%
CGTCATTTGATGAATTCTTC+GGG	-	chr8_1:40936130-40936149	None:intergenic	35.0%
CAAATGACGTTGAGTAACTT+AGG	+	chr8_1:40936141-40936160	Msa1193960:CDS	35.0%
TATAGGCAACAAAGCAACTA+AGG	+	chr8_1:40936458-40936477	Msa1193960:intron	35.0%
AGGATATCTAGCACTATTGT+TGG	+	chr8_1:40936487-40936506	Msa1193960:CDS	35.0%
CGAATATCTTGAGAAACCAT+TGG	+	chr8_1:40936573-40936592	Msa1193960:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	40936060	40936678	40936060	ID=Msa1193960;Name=Msa1193960
chr8_1	mRNA	40936060	40936678	40936060	ID=Msa1193960-mRNA-1;Parent=Msa1193960;Name=Msa1193960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|0\|0\|1\|1\|1\|2\|0\|144
chr8_1	exon	40936060	40936278	40936060	ID=Msa1193960-mRNA-1:exon:13208;Parent=Msa1193960-mRNA-1
chr8_1	exon	40936463	40936678	40936463	ID=Msa1193960-mRNA-1:exon:13209;Parent=Msa1193960-mRNA-1
chr8_1	CDS	40936060	40936278	40936060	ID=Msa1193960-mRNA-1:cds;Parent=Msa1193960-mRNA-1
chr8_1	CDS	40936463	40936678	40936463	ID=Msa1193960-mRNA-1:cds;Parent=Msa1193960-mRNA-1

Gene Sequence

>Msa1193960

ATGGCATCACCATCTCAAAACTTTACATCCAAGTTAACAGCACTTGTTGTGGATGATGATGCAATGACCCGAAGAATTCATCAAATGACGTTGAGTAACTTAGGAGTTAACAACACTTGTGTGAAAAACGGAAAAGAAGCAGTGGAAATTCATCAGCATGGTAAATTTTTTGACCTAATCTTGATGGATAAGGATATGCCTGAGATGAATGGAGCTGAGGCAACAAAGCAACTAAGGTTGATGAGGATATCTAGCACTATTGTTGGTGTATCCTCACACTCTATGGAAGAGGAGGAAATAGTGATAAAATTTATGGAAGCAGGACTCGACGAATATCTTGAGAAACCATTGGATAAGGCAAAACTCAATTCCATTCTTCATGCCGTTGGAAAAATAAAAGAAAAAGAAAATTCCATTCCTCACAAGATGAAATGA

Protein sequence

>Msa1193960

MASPSQNFTSKLTALVVDDDAMTRRIHQMTLSNLGVNNTCVKNGKEAVEIHQHGKFFDLILMDKDMPEMNGAEATKQLRLMRISSTIVGVSSHSMEEEEIVIKFMEAGLDEYLEKPLDKAKLNSILHAVGKIKEKENSIPHKMK*