AlfalfaGEDB | Msa1195210

M. sativa cultivar zhongmu-4 / Msa1195210

KEGG: K11644
GO: -
PFAM: PF02671.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1195210	sp\|O48686\|SNL3_ARATH	39.196	199	84	2	1	199	1	162	2.76e-35	134

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1195210	G7LEM6	91.626	203	15	2	1	201	1	203	7.64e-130	372

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TFs/TRs:

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Gene ID	Type	Classification
Msa1195210	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1195210	MtrunA17_Chr8g0364021	76.923	182	32	4	1	172	31	212	2.09e-90	265
Msa1195210	MtrunA17_Chr5g0415031	39.286	196	82	2	4	199	33	191	6.86e-38	139
Msa1195210	MtrunA17_Chr6g0452001	41.206	199	75	4	2	199	54	211	1.29e-37	138
Msa1195210	MtrunA17_Chr8g0364231	38.660	194	82	2	6	199	34	190	4.79e-35	130
Msa1195210	MtrunA17_Chr6g0452021	38.021	192	80	2	8	199	37	189	6.23e-34	125
Msa1195210	MtrunA17_Chr1g0206781	47.191	89	46	1	8	96	5	92	1.07e-19	80.5
Msa1195210	MtrunA17_Chr1g0206771	51.389	72	35	0	7	78	9	80	4.67e-18	78.2
Msa1195210	MtrunA17_Chr1g0206771	48.571	70	36	0	13	82	94	163	2.08e-12	62.8
Msa1195210	MtrunA17_Chr3g0077881	43.119	109	55	3	8	109	2	110	3.96e-17	75.5
Msa1195210	MtrunA17_Chr3g0077881	43.529	85	46	2	5	87	90	174	3.55e-12	62.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1195210	AT1G24190.3	39.691	194	80	2	6	199	6	162	1.97e-36	135
Msa1195210	AT1G24190.1	39.196	199	84	2	1	199	1	162	2.81e-36	134
Msa1195210	AT1G24190.2	39.196	199	84	2	1	199	1	162	2.86e-36	134
Msa1195210	AT1G70060.2	38.191	199	87	2	1	199	1	163	1.49e-35	132
Msa1195210	AT1G70060.1	38.191	199	87	2	1	199	1	163	1.49e-35	132
Msa1195210	AT3G01320.2	37.629	194	83	1	7	200	50	205	2.39e-35	132
Msa1195210	AT3G01320.1	37.629	194	83	1	7	200	50	205	2.61e-35	132
Msa1195210	AT5G15020.2	36.979	192	82	3	8	199	46	198	6.37e-32	122
Msa1195210	AT5G15020.1	36.979	192	82	3	8	199	46	198	7.51e-32	122
Msa1195210	AT1G59890.3	36.559	186	79	2	14	199	45	191	2.46e-31	120

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Find 39 sgRNAs with CRISPR-Local

Find 328 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATGCTAATGAGTTTGTC+AGG	0.450168	8_1:-43030074	Msa1195210:CDS
AAGATGAGTACGAGTCAAGG+TGG	0.711763	8_1:-43030750	Msa1195210:CDS
ACAAACTCATTAGCATGTTC+AGG	0.410073	8_1:+43030078	None:intergenic
ACAAAGATGAGTACGAGTCA+AGG	0.614659	8_1:-43030753	None:intergenic
ACATTCATCAAGATTCCTAG+GGG	0.685325	8_1:+43030212	None:intergenic
ACTTCCACTTCTTAAACACC+AGG	0.607178	8_1:+43026418	None:intergenic
AGCATATCACAACTCCATCA+AGG	0.566686	8_1:-43030119	Msa1195210:CDS
AGGACATAATGATCTGATTA+CGG	0.410966	8_1:-43030295	Msa1195210:CDS
ATATGCAAAGGCATCATCTT+CGG	0.504699	8_1:+43030716	None:intergenic
ATGTGAAATGACAATTCCAT+TGG	0.573688	8_1:-43030193	Msa1195210:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAGGATTACAAAGATAAC+AGG	-	chr8_1:43026547-43026566	Msa1195210:intron	30.0%
GATTACAAAGATAACAGGTT+AGG	-	chr8_1:43026552-43026571	Msa1195210:intron	30.0%
TAATTACCATGACGTTGTAA+CGG	-	chr8_1:43026839-43026858	Msa1195210:intron	30.0%
AGGACATAATGATCTGATTA+CGG	-	chr8_1:43026881-43026900	Msa1195210:intron	30.0%
ATGTGAAATGACAATTCCAT+TGG	-	chr8_1:43026983-43027002	Msa1195210:intron	30.0%
TCTAATAAAGAAGAAGCTAC+TGG	-	chr8_1:43027301-43027320	Msa1195210:intron	30.0%
CTAATAAAGAAGAAGCTACT+GGG	-	chr8_1:43027302-43027321	Msa1195210:intron	30.0%
AACTCAAACATTCATCTAGT+CGG	-	chr8_1:43027449-43027468	Msa1195210:intron	30.0%
TGCATTGTTCTATTGCTAAT+AGG	-	chr8_1:43027540-43027559	Msa1195210:intron	30.0%
TGTTCTATTGCTAATAGGTT+TGG	-	chr8_1:43027545-43027564	Msa1195210:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	43026429	43030769	43026429	ID=Msa1195210;Name=Msa1195210
chr8_1	mRNA	43026429	43030769	43026429	ID=Msa1195210-mRNA-1;Parent=Msa1195210;Name=Msa1195210-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|0\|0\|1\|1\|1\|4\|0\|201
chr8_1	exon	43030630	43030769	43030630	ID=Msa1195210-mRNA-1:exon:13898;Parent=Msa1195210-mRNA-1
chr8_1	exon	43030065	43030362	43030065	ID=Msa1195210-mRNA-1:exon:13897;Parent=Msa1195210-mRNA-1
chr8_1	exon	43026635	43026733	43026635	ID=Msa1195210-mRNA-1:exon:13896;Parent=Msa1195210-mRNA-1
chr8_1	exon	43026429	43026497	43026429	ID=Msa1195210-mRNA-1:exon:13895;Parent=Msa1195210-mRNA-1
chr8_1	CDS	43030630	43030769	43030630	ID=Msa1195210-mRNA-1:cds;Parent=Msa1195210-mRNA-1
chr8_1	CDS	43030065	43030362	43030065	ID=Msa1195210-mRNA-1:cds;Parent=Msa1195210-mRNA-1
chr8_1	CDS	43026635	43026733	43026635	ID=Msa1195210-mRNA-1:cds;Parent=Msa1195210-mRNA-1
chr8_1	CDS	43026429	43026497	43026429	ID=Msa1195210-mRNA-1:cds;Parent=Msa1195210-mRNA-1

Gene Sequence

>Msa1195210

ATGAGTACGAGTCAAGGTGGTAAGAAACTAACCGAAGATGATGCCTTTGCATATCTTAAGGCAGTCAAGGACGTTTTCAGAGAACAAATGGAAAACTATTCTTACTTTTTGAAAATAATGAAGGATTACAAAGATAACAGGATTAATTACCATGACGTTGTAACGGAGGTGAAGGAGTTGTTTAAAGGACATAATGATCTGATTACGGGATTCAACATCTTTGCACCAAGTGGTTATCAAATCCCAATTTCAGAAGAAGATGAACAACCCCCTAGGAATCTTGATGAATGTGAAATGACAATTCCATTGGAAGAATTAAAACCTCTCAATGCACAATCTCCTCAGATGATGCTTGGAGAAGAGCATATCACAACTCCATCAAGGAATGAACCATTTGGTGGACCTGAACATGCTAATGAGTTTGTCAGGAAAGTTAAGACTCGGTATGAAGGTGATCGTCATGTTTTCAAGTCATTTCTTTACATCATGAACATGCTGATAGATAGGAAAAAGACTTACATAGAGACTTACTATGAGGCTGCTGCACTTTTGGAGGGTCACACAGATCTTCAAGATGAGTTAATTATGTTTTTTCCTGGTGTTTAA

Protein sequence

>Msa1195210

MSTSQGGKKLTEDDAFAYLKAVKDVFREQMENYSYFLKIMKDYKDNRINYHDVVTEVKELFKGHNDLITGFNIFAPSGYQIPISEEDEQPPRNLDECEMTIPLEELKPLNAQSPQMMLGEEHITTPSRNEPFGGPEHANEFVRKVKTRYEGDRHVFKSFLYIMNMLIDRKKTYIETYYEAAALLEGHTDLQDELIMFFPGV*