AlfalfaGEDB | Msa1199960

M. sativa cultivar zhongmu-4 / Msa1199960

KEGG: K08829
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199960	sp\|Q6Z8C8\|CDKF4_ORYSJ	57.543	464	177	7	1	450	1	458	0.0	531

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199960	A0A072TT15	98.004	451	9	0	1	451	1	451	0.0	920

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199960	MtrunA17_Chr8g0369451	97.783	451	9	1	1	451	1	450	0.0	913
Msa1199960	MtrunA17_Chr5g0409421	78.714	451	94	1	1	451	1	449	0.0	761
Msa1199960	MtrunA17_Chr8g0339221	55.782	294	129	1	1	293	1	294	1.06e-120	359
Msa1199960	MtrunA17_Chr4g0051261	37.037	297	177	6	1	290	1	294	4.81e-68	219
Msa1199960	MtrunA17_Chr2g0293491	36.700	297	178	6	1	290	1	294	2.27e-67	217
Msa1199960	MtrunA17_Chr5g0397261	38.832	291	161	6	7	285	44	329	1.41e-62	207
Msa1199960	MtrunA17_Chr8g0378831	39.933	298	161	9	3	287	31	323	1.22e-61	204
Msa1199960	MtrunA17_Chr3g0099951	36.082	291	177	5	2	286	12	299	1.21e-60	203
Msa1199960	MtrunA17_Chr3g0141871	38.305	295	170	7	3	287	31	323	7.42e-60	200
Msa1199960	MtrunA17_Chr7g0248461	37.847	288	164	6	7	283	41	324	8.52e-60	199

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199960	AT4G19110.1	67.957	465	131	8	1	451	1	461	0.0	653
Msa1199960	AT4G19110.2	67.521	468	131	8	1	451	1	464	0.0	652
Msa1199960	AT5G45430.2	57.654	503	155	8	1	451	1	497	0.0	563
Msa1199960	AT5G45430.1	57.030	505	157	8	1	451	1	499	0.0	560
Msa1199960	AT4G19110.3	65.441	408	124	8	57	451	1	404	0.0	547
Msa1199960	AT4G13020.4	59.790	286	109	2	1	283	1	283	3.04e-129	380
Msa1199960	AT4G13020.7	59.790	286	109	2	1	283	1	283	3.93e-129	378
Msa1199960	AT4G13020.6	59.790	286	109	2	1	283	1	283	4.17e-129	379
Msa1199960	AT4G13020.1	59.790	286	109	2	1	283	1	283	8.52e-129	380
Msa1199960	AT4G13020.2	59.441	286	110	2	1	283	9	291	2.75e-127	376

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Find 218 sgRNAs with CRISPR-Local

Find 537 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGTTTGGTGATTGG+TGG	0.518617	8_1:-52261440	Msa1199960:five_prime_UTR
AAAAGGGCAGAAAAGAGGTT+TGG	0.509955	8_1:-52261450	Msa1199960:five_prime_UTR
AAAATATTAAAGATATCTGC+TGG	0.534904	8_1:+52258538	None:intergenic
AAACAAAAGAAAAGAGAAAA+AGG	0.181570	8_1:-52261467	None:intergenic
AAACCGGAATAAATTCTCCA+AGG	0.625894	8_1:+52259349	None:intergenic
AAAGTTCTTCCAGTTGGAAT+CGG	0.398901	8_1:+52254299	None:intergenic
AAATTACATACCAGATGGAG+GGG	0.600758	8_1:+52255654	None:intergenic
AAATTGGTGATGGAACATTT+GGG	0.480090	8_1:-52258324	Msa1199960:CDS
AACAAGGGCAGAACTGCTCG+TGG	0.655220	8_1:-52254464	Msa1199960:CDS
AACCCGTCCACCTCCAATGA+AGG	0.517538	8_1:-52254375	Msa1199960:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAATTGAATCGAAAGAGAA+TGG	+	chr8_1:52253845-52253864	None:intergenic	30.0%
TCCTCGATCCAAAAAAAAAA+AGG	+	chr8_1:52253916-52253935	None:intergenic	30.0%
TCGTTTCGTGATTTATGAAT+TGG	-	chr8_1:52253943-52253962	Msa1199960:three_prime_UTR	30.0%
TTCGTGATTTATGAATTGGT+AGG	-	chr8_1:52253947-52253966	Msa1199960:three_prime_UTR	30.0%
TTTCTCGATTTATCATTGGA+AGG	-	chr8_1:52254010-52254029	Msa1199960:three_prime_UTR	30.0%
AGAATAAGGTCTCAATTAAG+TGG	-	chr8_1:52254111-52254130	Msa1199960:three_prime_UTR	30.0%
AAAATAGATGACATTGAGAG+AGG	-	chr8_1:52254598-52254617	Msa1199960:intron	30.0%
TATTACTCCTCTCCATTTAT+AGG	-	chr8_1:52254922-52254941	Msa1199960:intron	30.0%
TAGTTAGTTAGTTAGTCCAT+GGG	+	chr8_1:52254997-52255016	None:intergenic	30.0%
TTAGTTAGTTAGTTAGTCCA+TGG	+	chr8_1:52254998-52255017	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	52253679	52261474	52253679	ID=Msa1199960;Name=Msa1199960
chr8_1	mRNA	52253679	52261474	52253679	ID=Msa1199960-mRNA-1;Parent=Msa1199960;Name=Msa1199960-mRNA-1;_AED=0.08;_eAED=0.10;_QI=1168\|1\|1\|1\|0.83\|0.78\|19\|598\|451
chr8_1	exon	52253679	52254494	52253679	ID=Msa1199960-mRNA-1:exon:16680;Parent=Msa1199960-mRNA-1
chr8_1	exon	52254829	52254904	52254829	ID=Msa1199960-mRNA-1:exon:16679;Parent=Msa1199960-mRNA-1
chr8_1	exon	52255075	52255106	52255075	ID=Msa1199960-mRNA-1:exon:16678;Parent=Msa1199960-mRNA-1
chr8_1	exon	52255427	52255543	52255427	ID=Msa1199960-mRNA-1:exon:16677;Parent=Msa1199960-mRNA-1
chr8_1	exon	52255665	52255725	52255665	ID=Msa1199960-mRNA-1:exon:16676;Parent=Msa1199960-mRNA-1
chr8_1	exon	52255858	52255926	52255858	ID=Msa1199960-mRNA-1:exon:16675;Parent=Msa1199960-mRNA-1
chr8_1	exon	52256014	52256079	52256014	ID=Msa1199960-mRNA-1:exon:16674;Parent=Msa1199960-mRNA-1
chr8_1	exon	52256629	52256737	52256629	ID=Msa1199960-mRNA-1:exon:16673;Parent=Msa1199960-mRNA-1
chr8_1	exon	52257000	52257066	52257000	ID=Msa1199960-mRNA-1:exon:16672;Parent=Msa1199960-mRNA-1
chr8_1	exon	52257182	52257234	52257182	ID=Msa1199960-mRNA-1:exon:16671;Parent=Msa1199960-mRNA-1
chr8_1	exon	52257329	52257431	52257329	ID=Msa1199960-mRNA-1:exon:16670;Parent=Msa1199960-mRNA-1
chr8_1	exon	52257733	52257871	52257733	ID=Msa1199960-mRNA-1:exon:16669;Parent=Msa1199960-mRNA-1
chr8_1	exon	52257955	52258044	52257955	ID=Msa1199960-mRNA-1:exon:16668;Parent=Msa1199960-mRNA-1
chr8_1	exon	52258131	52258199	52258131	ID=Msa1199960-mRNA-1:exon:16667;Parent=Msa1199960-mRNA-1
chr8_1	exon	52258285	52258363	52258285	ID=Msa1199960-mRNA-1:exon:16666;Parent=Msa1199960-mRNA-1
chr8_1	exon	52258451	52259027	52258451	ID=Msa1199960-mRNA-1:exon:16665;Parent=Msa1199960-mRNA-1
chr8_1	exon	52259116	52259167	52259116	ID=Msa1199960-mRNA-1:exon:16664;Parent=Msa1199960-mRNA-1
chr8_1	exon	52259241	52259501	52259241	ID=Msa1199960-mRNA-1:exon:16663;Parent=Msa1199960-mRNA-1
chr8_1	exon	52261189	52261474	52261189	ID=Msa1199960-mRNA-1:exon:16662;Parent=Msa1199960-mRNA-1
chr8_1	five_prime_UTR	52261189	52261474	52261189	ID=Msa1199960-mRNA-1:five_prime_utr;Parent=Msa1199960-mRNA-1
chr8_1	five_prime_UTR	52259241	52259501	52259241	ID=Msa1199960-mRNA-1:five_prime_utr;Parent=Msa1199960-mRNA-1
chr8_1	five_prime_UTR	52259116	52259167	52259116	ID=Msa1199960-mRNA-1:five_prime_utr;Parent=Msa1199960-mRNA-1
chr8_1	five_prime_UTR	52258459	52259027	52258459	ID=Msa1199960-mRNA-1:five_prime_utr;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52258451	52258458	52258451	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52258285	52258363	52258285	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52258131	52258199	52258131	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52257955	52258044	52257955	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52257733	52257871	52257733	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52257329	52257431	52257329	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52257182	52257234	52257182	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52257000	52257066	52257000	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52256629	52256737	52256629	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52256014	52256079	52256014	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52255858	52255926	52255858	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52255665	52255725	52255665	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52255427	52255543	52255427	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52255075	52255106	52255075	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52254829	52254904	52254829	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	CDS	52254277	52254494	52254277	ID=Msa1199960-mRNA-1:cds;Parent=Msa1199960-mRNA-1
chr8_1	three_prime_UTR	52253679	52254276	52253679	ID=Msa1199960-mRNA-1:three_prime_utr;Parent=Msa1199960-mRNA-1

Gene Sequence

>Msa1199960

ATGGAAAGGTACAAATTAATTAAGGAAATTGGTGATGGAACATTTGGGATTGTCTGGAGAGCCATTAATAAGCAAAGCGGTGAAGTTGTTGCTATCAAGAAAATGAAGAAAAAATATTACTCTTGGGAGGAGTGTGTAAACCTGAGAGAAGTCAAGTCATTACGAAAAATGAATCACTCAAATGTCGTGAAGCTGAAGGAAGTTATTCGAGAAAGTGACATTCTGTACCTTGTTTTTGAATACATGGAATGCAACCTCTACCAACTTATGAAAAAAAGGGAAAAGCTGTTTTCTGATGATGAAGTTAGAAATTTGTGTTTTCAAGTTTTTCAAGGTCTTGCATACATGCACCAACGTGGATACTTCCATCGTGATCTAAAACCTGAAAACTTGTTAGTCACCAAGGATATTATTAAAGTTTCTGATTTCGGCCTAGTGCGAGAGATCAGCTCACAACCACCCTATACTGAGTATGTCTCCACACGGTGGTATCGTGCTCCTGAAGTCCTGCTTCAGTCTTCCCTTTATAGTTCCAAAGTTGACATGTGGGCTATGGGTGCTATTATGGCTGAATTGTTCACACTCCGGCCTCTATTTCCGGGTACCAGTGAAGCAGATGAGATCTACAAAATTTGCAGTGTGATAGGTAGTCCAACCGCTGAATCTTGGGCTGATGGACTCAAACTTGCTAGGGATATCAACTATCAATTTCCACAGGTAGCTACTGCAGATCTCTCTGTACTGATACCATCCAGAAGCGATGATGCAATCAACCTTATCAAATTACTTTGTTCATGGGATCCCTGCAATAGACCTTCTGCAGCAGAGGCCCTTCAACATCCTTTCTTCAAGAGTTGCTTTTACATTCCTCCATCGCTTCGCACCAAAGTAGTAACAAGAACCCCTCCATCTGCTGGAGTAAGGGGATCAGTAGATCGGCAAGGGGTCAAGAGATATTCTGGTGGATTGTCTAATTCGAAACTGACCACTAATTTTTCTTCCAACAAATTACATCCTGCTTTAGCTCCAGGTGTGCAAAGGAAACTGGATATGGCAAATGAGGATGGAACTAAAAATAAGAAAACAGTGAAGACTAATCAACAATCAAAATATCGACCGCCAGGAAAAGACAGTCCGACTTCTATAAACAAGGGCAGAACTGCTCGTGGTGTTTCAGAAACAGCCAACAAGTTGGCCAACATGTCAATTGCTACTCGAAGACAGTCATTGGGTCAAACCCGTCCACCTCCAATGAAGGCCGGAGTTAACTGGACTTCTGAATCTGGAAACATCCTGCTCAGGTCCTCCCACCCGATTCCAACTGGAAGAACTTTCACAAAAAAAGTTGCTGGATGA

Protein sequence

>Msa1199960

MERYKLIKEIGDGTFGIVWRAINKQSGEVVAIKKMKKKYYSWEECVNLREVKSLRKMNHSNVVKLKEVIRESDILYLVFEYMECNLYQLMKKREKLFSDDEVRNLCFQVFQGLAYMHQRGYFHRDLKPENLLVTKDIIKVSDFGLVREISSQPPYTEYVSTRWYRAPEVLLQSSLYSSKVDMWAMGAIMAELFTLRPLFPGTSEADEIYKICSVIGSPTAESWADGLKLARDINYQFPQVATADLSVLIPSRSDDAINLIKLLCSWDPCNRPSAAEALQHPFFKSCFYIPPSLRTKVVTRTPPSAGVRGSVDRQGVKRYSGGLSNSKLTTNFSSNKLHPALAPGVQRKLDMANEDGTKNKKTVKTNQQSKYRPPGKDSPTSINKGRTARGVSETANKLANMSIATRRQSLGQTRPPPMKAGVNWTSESGNILLRSSHPIPTGRTFTKKVAG*