Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1201930 | KVH97760.1 | 84.000 | 75 | 12 | 0 | 12 | 86 | 103 | 177 | 1.72e-31 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1201930 | sp|Q9LVS0|KUA1_ARATH | 75.000 | 72 | 18 | 0 | 12 | 83 | 94 | 165 | 1.51e-34 | 124 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1201930 | A0A444XY94 | 86.111 | 72 | 10 | 0 | 12 | 83 | 76 | 147 | 9.01e-37 | 134 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1201930 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1201930 | MtrunA17_Chr3g0092621 | 69.663 | 89 | 23 | 1 | 1 | 85 | 67 | 155 | 4.31e-38 | 125 |
| Msa1201930 | MtrunA17_Chr4g0055931 | 74.286 | 70 | 18 | 0 | 12 | 81 | 93 | 162 | 1.35e-34 | 120 |
| Msa1201930 | MtrunA17_Chr2g0331031 | 70.833 | 72 | 21 | 0 | 12 | 83 | 107 | 178 | 1.40e-34 | 120 |
| Msa1201930 | MtrunA17_Chr6g0488471 | 74.627 | 67 | 17 | 0 | 12 | 78 | 95 | 161 | 1.90e-33 | 116 |
| Msa1201930 | MtrunA17_Chr5g0415191 | 83.562 | 73 | 12 | 0 | 12 | 84 | 100 | 172 | 3.69e-33 | 116 |
| Msa1201930 | MtrunA17_Chr8g0389241 | 73.134 | 67 | 17 | 1 | 12 | 78 | 103 | 168 | 5.47e-31 | 109 |
| Msa1201930 | MtrunA17_Chr4g0059701 | 91.667 | 72 | 6 | 0 | 12 | 83 | 82 | 153 | 5.53e-31 | 110 |
| Msa1201930 | MtrunA17_Chr4g0062741 | 71.014 | 69 | 20 | 0 | 12 | 80 | 136 | 204 | 1.37e-30 | 109 |
| Msa1201930 | MtrunA17_Chr1g0210471 | 68.116 | 69 | 22 | 0 | 12 | 80 | 122 | 190 | 5.64e-30 | 106 |
| Msa1201930 | MtrunA17_Chr1g0162081 | 69.565 | 69 | 21 | 0 | 12 | 80 | 120 | 188 | 1.34e-29 | 106 |
| Msa1201930 | MtrunA17_Chr3g0104071 | 69.565 | 69 | 21 | 0 | 12 | 80 | 145 | 213 | 2.05e-29 | 106 |
| Msa1201930 | MtrunA17_Chr5g0429521 | 69.565 | 69 | 21 | 0 | 12 | 80 | 130 | 198 | 2.67e-29 | 105 |
| Msa1201930 | MtrunA17_Chr7g0241761 | 69.118 | 68 | 21 | 0 | 12 | 79 | 138 | 205 | 1.18e-28 | 104 |
| Msa1201930 | MtrunA17_Chr6g0452791 | 66.176 | 68 | 23 | 0 | 6 | 73 | 72 | 139 | 1.02e-26 | 96.3 |
| Msa1201930 | MtrunA17_Chr5g0436501 | 62.162 | 74 | 26 | 1 | 12 | 83 | 123 | 196 | 7.94e-26 | 96.3 |
| Msa1201930 | MtrunA17_Chr3g0135671 | 58.667 | 75 | 28 | 1 | 10 | 81 | 87 | 161 | 1.15e-24 | 92.4 |
| Msa1201930 | MtrunA17_Chr5g0440731 | 45.783 | 83 | 39 | 2 | 12 | 88 | 103 | 185 | 1.34e-18 | 77.4 |
| Msa1201930 | MtrunA17_Chr5g0440741 | 46.835 | 79 | 36 | 2 | 16 | 88 | 102 | 180 | 9.43e-18 | 75.1 |
| Msa1201930 | MtrunA17_Chr5g0409841 | 48.485 | 66 | 32 | 1 | 16 | 79 | 94 | 159 | 2.06e-17 | 73.6 |
| Msa1201930 | MtrunA17_Chr5g0409831 | 49.333 | 75 | 32 | 2 | 16 | 84 | 79 | 153 | 3.29e-16 | 70.1 |
| Msa1201930 | MtrunA17_Chr8g0364731 | 57.627 | 59 | 23 | 1 | 23 | 79 | 10 | 68 | 7.54e-16 | 68.6 |
| Msa1201930 | MtrunA17_Chr8g0365011 | 44.872 | 78 | 29 | 2 | 16 | 79 | 94 | 171 | 5.05e-15 | 67.8 |
| Msa1201930 | MtrunA17_Chr1g0192061 | 47.143 | 70 | 33 | 2 | 16 | 82 | 63 | 131 | 3.10e-13 | 61.6 |
| Msa1201930 | MtrunA17_Chr3g0113121 | 44.444 | 72 | 33 | 1 | 16 | 80 | 97 | 168 | 9.64e-12 | 58.2 |
| Msa1201930 | MtrunA17_Chr5g0432921 | 46.269 | 67 | 33 | 2 | 16 | 80 | 115 | 180 | 2.03e-11 | 57.4 |
| Msa1201930 | MtrunA17_Chr5g0432941 | 44.776 | 67 | 34 | 2 | 16 | 80 | 115 | 180 | 6.96e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1201930 | AT5G47390.1 | 75.000 | 72 | 18 | 0 | 12 | 83 | 94 | 165 | 1.53e-35 | 124 |
| Msa1201930 | AT1G49010.1 | 72.464 | 69 | 19 | 0 | 12 | 80 | 132 | 200 | 2.01e-31 | 112 |
| Msa1201930 | AT1G70000.1 | 78.947 | 76 | 16 | 0 | 12 | 87 | 96 | 171 | 7.51e-31 | 109 |
| Msa1201930 | AT1G70000.2 | 78.947 | 76 | 16 | 0 | 12 | 87 | 96 | 171 | 7.51e-31 | 109 |
| Msa1201930 | AT5G04760.1 | 68.116 | 69 | 22 | 0 | 12 | 80 | 97 | 165 | 3.53e-30 | 106 |
| Msa1201930 | AT2G38090.1 | 69.565 | 69 | 21 | 0 | 12 | 80 | 139 | 207 | 6.67e-30 | 107 |
| Msa1201930 | AT5G58900.1 | 69.565 | 69 | 21 | 0 | 12 | 80 | 139 | 207 | 9.83e-30 | 107 |
| Msa1201930 | AT5G08520.1 | 68.116 | 69 | 22 | 0 | 12 | 80 | 116 | 184 | 1.73e-29 | 106 |
| Msa1201930 | AT5G56840.1 | 65.714 | 70 | 23 | 1 | 12 | 80 | 89 | 158 | 5.97e-29 | 103 |
| Msa1201930 | AT5G61620.1 | 64.286 | 70 | 25 | 0 | 12 | 81 | 106 | 175 | 8.05e-29 | 105 |
| Msa1201930 | AT1G74840.2 | 83.562 | 73 | 12 | 0 | 12 | 84 | 94 | 166 | 1.12e-28 | 103 |
| Msa1201930 | AT1G74840.1 | 83.562 | 73 | 12 | 0 | 12 | 84 | 94 | 166 | 1.48e-28 | 103 |
| Msa1201930 | AT1G19000.2 | 74.699 | 83 | 21 | 0 | 1 | 83 | 89 | 171 | 1.65e-27 | 101 |
| Msa1201930 | AT1G19000.1 | 74.699 | 83 | 21 | 0 | 1 | 83 | 89 | 171 | 1.65e-27 | 101 |
| Msa1201930 | AT3G16350.1 | 68.493 | 73 | 23 | 0 | 12 | 84 | 135 | 207 | 2.93e-27 | 102 |
| Msa1201930 | AT5G01200.1 | 65.217 | 69 | 24 | 0 | 12 | 80 | 144 | 212 | 8.20e-27 | 99.0 |
| Msa1201930 | AT5G05790.2 | 56.410 | 78 | 34 | 0 | 12 | 89 | 129 | 206 | 1.06e-25 | 96.3 |
| Msa1201930 | AT5G05790.1 | 56.410 | 78 | 34 | 0 | 12 | 89 | 129 | 206 | 1.06e-25 | 96.3 |
| Msa1201930 | AT3G11280.2 | 58.904 | 73 | 30 | 0 | 12 | 84 | 125 | 197 | 1.74e-25 | 95.5 |
| Msa1201930 | AT3G11280.1 | 58.904 | 73 | 30 | 0 | 12 | 84 | 125 | 197 | 1.74e-25 | 95.5 |
| Msa1201930 | AT3G10580.1 | 57.353 | 68 | 27 | 1 | 12 | 79 | 93 | 158 | 2.80e-23 | 90.1 |
| Msa1201930 | AT3G10580.2 | 57.353 | 68 | 27 | 1 | 12 | 79 | 93 | 158 | 9.43e-23 | 88.2 |
| Msa1201930 | AT4G09450.1 | 52.778 | 72 | 34 | 0 | 13 | 84 | 90 | 161 | 2.46e-22 | 85.9 |
| Msa1201930 | AT5G23650.1 | 57.353 | 68 | 29 | 0 | 12 | 79 | 118 | 185 | 3.38e-21 | 85.5 |
| Msa1201930 | AT3G10590.1 | 50.725 | 69 | 33 | 1 | 15 | 83 | 112 | 179 | 1.17e-17 | 73.9 |
Find 19 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTTGGATTGAGCATTAA+AGG | 0.190813 | 8_1:+55233007 | Msa1201930:CDS |
| AGAAAGTGGGAAAAGGTGAT+TGG | 0.465565 | 8_1:+55233301 | Msa1201930:CDS |
| TTGGTTGGATTGAAGAAAGT+GGG | 0.486765 | 8_1:+55233288 | Msa1201930:CDS |
| ATGCAAGAGGAACTTTCACT+TGG | 0.526843 | 8_1:+55232992 | Msa1201930:CDS |
| TTTGGTTGGATTGAAGAAAG+TGG | 0.534445 | 8_1:+55233287 | Msa1201930:CDS |
| GGTGGTGATATCAAAGAGAC+TGG | 0.540239 | 8_1:-55233433 | None:intergenic |
| GGATTGAAGAAAGTGGGAAA+AGG | 0.562648 | 8_1:+55233294 | Msa1201930:CDS |
| TTGTGCTCTTCCTCAGTCCA+CGG | 0.578432 | 8_1:-55233258 | None:intergenic |
| TATCAAAGAGACTGGATCTA+CGG | 0.592657 | 8_1:-55233425 | None:intergenic |
| ATAACTACATCTTATGCAAG+AGG | 0.597216 | 8_1:+55232979 | None:intergenic |
| GTGATTGGAGAGGAATCTCA+AGG | 0.619264 | 8_1:+55233316 | Msa1201930:CDS |
| GTGGGAAAAGGTGATTGGAG+AGG | 0.624127 | 8_1:+55233306 | Msa1201930:CDS |
| TGAATGGATCAGGGATTCCG+TGG | 0.626840 | 8_1:+55233241 | Msa1201930:intron |
| GGCGATTGAGATTACTTCGG+CGG | 0.629813 | 8_1:-55233398 | None:intergenic |
| GCATGACTTGCAACATGCGT+AGG | 0.635692 | 8_1:-55233360 | None:intergenic |
| CTTTGATATCACCACCGACA+CGG | 0.638033 | 8_1:+55233440 | Msa1201930:CDS |
| GACGGCGATTGAGATTACTT+CGG | 0.641222 | 8_1:-55233401 | None:intergenic |
| ATCAGGGATTCCGTGGACTG+AGG | 0.660333 | 8_1:+55233248 | Msa1201930:intron |
| AGAGACTGGATCTACGGCGA+CGG | 0.684123 | 8_1:-55233419 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTCAATCAATTCAAACAA+AGG | - | chr8_1:55233191-55233210 | None:intergenic | 20.0% |
| !!! | TGTTATTGATTTTCTTTGAA+TGG | + | chr8_1:55233225-55233244 | Msa1201930:intron | 20.0% |
| ! | AATATCTACTTAAAATCCTG+AGG | - | chr8_1:55233040-55233059 | None:intergenic | 25.0% |
| !! | TCTCACATTTAAATGGTTTT+TGG | + | chr8_1:55233096-55233115 | Msa1201930:intron | 25.0% |
| CTACATGTCTCACATTTAAA+TGG | + | chr8_1:55233089-55233108 | Msa1201930:intron | 30.0% | |
| ! | TGATTTTCTTTGAATGGATC+AGG | + | chr8_1:55233231-55233250 | Msa1201930:intron | 30.0% |
| ! | GATTTTCTTTGAATGGATCA+GGG | + | chr8_1:55233232-55233251 | Msa1201930:intron | 30.0% |
| TCACTTGGATTGAGCATTAA+AGG | + | chr8_1:55233007-55233026 | Msa1201930:CDS | 35.0% | |
| CATTAAAGGTTGTTTACCTC+AGG | + | chr8_1:55233021-55233040 | Msa1201930:intron | 35.0% | |
| TTTGGTTGGATTGAAGAAAG+TGG | + | chr8_1:55233287-55233306 | Msa1201930:CDS | 35.0% | |
| TTGGTTGGATTGAAGAAAGT+GGG | + | chr8_1:55233288-55233307 | Msa1201930:CDS | 35.0% | |
| TATCAAAGAGACTGGATCTA+CGG | - | chr8_1:55233428-55233447 | None:intergenic | 35.0% | |
| ATGCAAGAGGAACTTTCACT+TGG | + | chr8_1:55232992-55233011 | Msa1201930:CDS | 40.0% | |
| CATTCTCTCTGAGCAAACAA+TGG | + | chr8_1:55233066-55233085 | Msa1201930:intron | 40.0% | |
| !! | GGAAGAGCACAAGATGTTTT+TGG | + | chr8_1:55233269-55233288 | Msa1201930:CDS | 40.0% |
| !!! | GAGCACAAGATGTTTTTGGT+TGG | + | chr8_1:55233273-55233292 | Msa1201930:CDS | 40.0% |
| GGATTGAAGAAAGTGGGAAA+AGG | + | chr8_1:55233294-55233313 | Msa1201930:CDS | 40.0% | |
| AGAAAGTGGGAAAAGGTGAT+TGG | + | chr8_1:55233301-55233320 | Msa1201930:CDS | 40.0% | |
| !!! | CACCGACACGGTAATTTTTT+CGG | + | chr8_1:55233452-55233471 | Msa1201930:CDS | 40.0% |
| TGCCGAAAAAATTACCGTGT+CGG | - | chr8_1:55233457-55233476 | None:intergenic | 40.0% | |
| GTGATTGGAGAGGAATCTCA+AGG | + | chr8_1:55233316-55233335 | Msa1201930:CDS | 45.0% | |
| GACGGCGATTGAGATTACTT+CGG | - | chr8_1:55233404-55233423 | None:intergenic | 45.0% | |
| GGTGGTGATATCAAAGAGAC+TGG | - | chr8_1:55233436-55233455 | None:intergenic | 45.0% | |
| CTTTGATATCACCACCGACA+CGG | + | chr8_1:55233440-55233459 | Msa1201930:CDS | 45.0% | |
| CGAAAAAATTACCGTGTCGG+TGG | - | chr8_1:55233454-55233473 | None:intergenic | 45.0% | |
| TGAATGGATCAGGGATTCCG+TGG | + | chr8_1:55233241-55233260 | Msa1201930:intron | 50.0% | |
| TTGTGCTCTTCCTCAGTCCA+CGG | - | chr8_1:55233261-55233280 | None:intergenic | 50.0% | |
| GTGGGAAAAGGTGATTGGAG+AGG | + | chr8_1:55233306-55233325 | Msa1201930:CDS | 50.0% | |
| ! | GCATGACTTGCAACATGCGT+AGG | - | chr8_1:55233363-55233382 | None:intergenic | 50.0% |
| GGCGATTGAGATTACTTCGG+CGG | - | chr8_1:55233401-55233420 | None:intergenic | 50.0% | |
| ! | ATCAGGGATTCCGTGGACTG+AGG | + | chr8_1:55233248-55233267 | Msa1201930:intron | 55.0% |
| AGAGACTGGATCTACGGCGA+CGG | - | chr8_1:55233422-55233441 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 55232992 | 55233485 | 55232992 | ID=Msa1201930;Name=Msa1201930 |
| chr8_1 | mRNA | 55232992 | 55233485 | 55232992 | ID=Msa1201930-mRNA-1;Parent=Msa1201930;Name=Msa1201930-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|0|0|0.5|0|0.5|2|0|89 |
| chr8_1 | exon | 55232992 | 55233028 | 55232992 | ID=Msa1201930-mRNA-1:exon:17870;Parent=Msa1201930-mRNA-1 |
| chr8_1 | exon | 55233253 | 55233485 | 55233253 | ID=Msa1201930-mRNA-1:exon:17871;Parent=Msa1201930-mRNA-1 |
| chr8_1 | CDS | 55232992 | 55233028 | 55232992 | ID=Msa1201930-mRNA-1:cds;Parent=Msa1201930-mRNA-1 |
| chr8_1 | CDS | 55233253 | 55233485 | 55233253 | ID=Msa1201930-mRNA-1:cds;Parent=Msa1201930-mRNA-1 |
| Gene Sequence |
| Protein sequence |
