AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1202090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1202090 MtrunA17_Chr8g0372461 96.736 337 5 3 1 337 1 331 0.0 664
Msa1202090 MtrunA17_Chr5g0404781 52.762 362 107 14 1 337 1 323 3.37e-91 275
Msa1202090 MtrunA17_Chr3g0142171 43.188 345 126 16 1 332 1 288 2.17e-63 203
Msa1202090 MtrunA17_Chr1g0155791 51.915 235 75 7 125 337 120 338 5.72e-58 190
Msa1202090 MtrunA17_Chr5g0441921 50.323 155 54 4 127 258 124 278 9.22e-41 145
Msa1202090 MtrunA17_Chr4g0035591 47.887 142 65 2 123 264 16 148 2.77e-40 140
Msa1202090 MtrunA17_Chr3g0082511 39.869 153 69 1 127 256 134 286 1.49e-33 126
Msa1202090 MtrunA17_Chr8g0353111 39.884 173 73 3 114 256 111 282 4.86e-33 125
Msa1202090 MtrunA17_Chr0c01g0489181 56.410 39 17 0 123 161 63 101 5.25e-14 67.4
Msa1202090 MtrunA17_Chr2g0281741 61.538 39 15 0 123 161 146 184 4.35e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1202090 AT3G51060.1 44.388 392 141 11 1 337 1 370 3.10e-80 249
Msa1202090 AT5G66350.1 44.624 372 130 14 1 337 1 331 6.15e-75 234
Msa1202090 AT5G66350.3 44.321 361 127 13 1 326 1 322 7.07e-73 229
Msa1202090 AT5G66350.2 44.444 360 126 13 1 325 1 321 1.05e-72 228
Msa1202090 AT1G19790.2 42.359 373 149 15 1 336 1 344 6.80e-69 219
Msa1202090 AT1G19790.1 42.359 373 149 15 1 336 1 344 6.80e-69 219
Msa1202090 AT1G75520.1 41.287 373 156 13 1 337 1 346 2.44e-63 205
Msa1202090 AT4G36260.1 39.722 360 151 13 1 335 1 319 2.04e-54 181
Msa1202090 AT2G21400.4 47.701 174 83 3 124 293 6 175 6.33e-49 162
Msa1202090 AT2G21400.2 47.701 174 83 3 124 293 7 176 6.60e-49 162
Msa1202090 AT2G21400.3 47.647 170 74 3 124 293 7 161 3.61e-48 160
Msa1202090 AT2G21400.1 47.647 170 74 3 124 293 6 160 3.66e-48 160
Msa1202090 AT2G18120.1 53.548 155 57 3 116 261 61 209 3.09e-47 159
Msa1202090 AT2G18120.2 52.288 153 60 2 116 261 61 207 1.88e-46 157
Msa1202090 AT5G12330.1 47.205 161 55 4 127 258 112 271 2.24e-38 139
Msa1202090 AT5G12330.2 47.205 161 55 4 127 258 112 271 2.24e-38 139
Msa1202090 AT5G12330.4 47.205 161 55 4 127 258 199 358 3.72e-38 140
Msa1202090 AT5G33210.1 42.405 158 70 5 93 235 14 165 4.50e-29 110
Msa1202090 AT5G33210.2 61.176 85 23 2 125 199 15 99 3.71e-27 103
Msa1202090 AT3G54430.1 42.553 141 59 6 127 256 41 170 1.09e-25 102
Msa1202090 AT5G12330.3 82.609 46 8 0 127 172 112 157 3.50e-21 90.9

Find 117 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTGTTGGTACTACTTTC+TGG 0.121760 8_1:-55558857 None:intergenic
TTAAAGAAACTACTGTTATT+AGG 0.143287 8_1:+55557343 Msa1202090:five_prime_UTR
AATGATTGAAACACAAATTT+TGG 0.146618 8_1:-55559181 None:intergenic
AAAGGAATTCTCTATGATTT+TGG 0.200894 8_1:+55558833 Msa1202090:CDS
AGAAGAAAGAGGAGTTATTA+TGG 0.207274 8_1:+55559114 Msa1202090:three_prime_UTR
ACAGTTGAGAGAGAATTAAT+TGG 0.214572 8_1:-55557237 None:intergenic
TTTCTATGTGTACTCTATAA+TGG 0.229112 8_1:+55559393 Msa1202090:three_prime_UTR
ACAGAGGTAATTTAGAATTA+TGG 0.238498 8_1:+55557523 Msa1202090:CDS
CACCACAAGAGACACTATTT+TGG 0.271662 8_1:+55557469 Msa1202090:CDS
TAAAGAAACTACTGTTATTA+GGG 0.274863 8_1:+55557344 Msa1202090:five_prime_UTR
TGATCCTTCTTCATTGTATT+CGG 0.283339 8_1:+55559018 Msa1202090:CDS
TATCATCTTGTTGTTGTTGC+TGG 0.328192 8_1:-55557581 None:intergenic
AATAATAGTAATTACATGAT+TGG 0.330195 8_1:+55558899 Msa1202090:CDS
GAAGAAAGAGGAGTTATTAT+GGG 0.330694 8_1:+55559115 Msa1202090:three_prime_UTR
ATAATAGTAATTACATGATT+GGG 0.347728 8_1:+55558900 Msa1202090:CDS
AACTACTGTTATTAGGGTTA+AGG 0.348943 8_1:+55557350 Msa1202090:five_prime_UTR
AAGTATATGTCTCCTTGTTA+TGG 0.356150 8_1:-55557125 None:intergenic
TACCACTGTTGTTGCCTCCT+CGG 0.375579 8_1:+55558985 Msa1202090:CDS
AAGCTATAGCTGCTGCTAGC+TGG 0.376069 8_1:+55556974 Msa1202090:five_prime_UTR
ATGGCTGGTTTGTTCTCATT+AGG 0.380675 8_1:+55557393 Msa1202090:CDS
TAAGCTCACCAGCGGAGTTC+AGG 0.381652 8_1:+55558729 Msa1202090:CDS
TACTCTATAATGGACTTCTA+TGG 0.385956 8_1:+55559403 Msa1202090:three_prime_UTR
CAGCGGAGTTCAGGTGTGTA+AGG 0.390315 8_1:+55558738 Msa1202090:CDS
GTTGTTGTGATTCTTCTCCT+TGG 0.392328 8_1:-55557434 None:intergenic
TACATGATTGGGGAGACTTC+CGG 0.393571 8_1:+55558911 Msa1202090:CDS
GTTGTTGCTGGTGGTGTTGT+TGG 0.401531 8_1:-55557569 None:intergenic
GTTGTTGGTGCTGTTGTTGT+TGG 0.405595 8_1:-55557554 None:intergenic
CAGTTGAGAGAGAATTAATT+GGG 0.406433 8_1:-55557236 None:intergenic
TACGGTCCAGGAGTTGGTTT+AGG 0.417465 8_1:+55557639 Msa1202090:CDS
ACCACTGTTGTTGCCTCCTC+GGG 0.418082 8_1:+55558986 Msa1202090:CDS
ACTATTGTTGCTGTTATTGT+TGG 0.418687 8_1:-55558870 None:intergenic
AGCTATAGCTGCTGCTAGCT+GGG 0.421738 8_1:+55556975 Msa1202090:five_prime_UTR
CCAGGAGTTGGTTTAGGTGT+TGG 0.426741 8_1:+55557645 Msa1202090:CDS
GTTTAGGTGTTGGACCAAGC+AGG 0.429835 8_1:+55557655 Msa1202090:CDS
GGTGCTGTTGTTGTTGGTGT+TGG 0.440333 8_1:-55557548 None:intergenic
TGGTATCAGAGTCGGTGATT+AGG 0.442544 8_1:-55558967 None:intergenic
TATGTCTCCTTGTTATGGTT+GGG 0.444674 8_1:-55557120 None:intergenic
GCCGTCATGAAGGCATTAAT+CGG 0.448399 8_1:-55559043 None:intergenic
ACTTCCGGTGGAGGCGGTGT+TGG 0.448651 8_1:+55558926 Msa1202090:CDS
ATAGAAAGAAACAATAGCTT+TGG 0.451472 8_1:-55559377 None:intergenic
ATTGGGGAGACTTCCGGTGG+AGG 0.461874 8_1:+55558917 Msa1202090:CDS
GAGGACAATCTTTCTTAGCT+TGG 0.464592 8_1:-55557788 None:intergenic
TTCTTCTTCAAAGAAATGGC+TGG 0.464677 8_1:+55557378 Msa1202090:exon
TTGTTCTCATTAGGAGGTGG+TGG 0.466253 8_1:+55557402 Msa1202090:CDS
TTAGAAATATCTCTTTGTAA+AGG 0.466509 8_1:-55557927 None:intergenic
GGGGAGACTTCCGGTGGAGG+CGG 0.470323 8_1:+55558920 Msa1202090:CDS
GAGATCGGCAGCGTTTACTA+TGG 0.472056 8_1:+55557713 Msa1202090:CDS
TTGAAGGAAGGAAGGAAGGA+AGG 0.475608 8_1:+55556951 Msa1202090:five_prime_UTR
AACGCCAACACCGCCTCCAC+CGG 0.479358 8_1:-55558930 None:intergenic
CAATTGATGATGCAGATGAT+AGG 0.486383 8_1:+55558768 Msa1202090:CDS
AATGAGATTATTATATATGA+AGG 0.489209 8_1:+55559275 Msa1202090:three_prime_UTR
TCATTAGGAGGTGGTGGAAG+AGG 0.489984 8_1:+55557408 Msa1202090:CDS
ATATGTCTCCTTGTTATGGT+TGG 0.492221 8_1:-55557121 None:intergenic
AAACTCATGTTAAAAGCACT+TGG 0.502171 8_1:+55557856 Msa1202090:CDS
ACATCAATCGTCGTCGAGAT+CGG 0.503948 8_1:+55557698 Msa1202090:CDS
AAACAAAATTGAGAAGAAAG+AGG 0.504247 8_1:+55559103 Msa1202090:three_prime_UTR
GGTTTGTTCTCATTAGGAGG+TGG 0.504419 8_1:+55557399 Msa1202090:CDS
GCTGGTGAGCTTACCACAGC+AGG 0.505962 8_1:-55558719 None:intergenic
CGCTCACGGCGTCTAGAAGC+AGG 0.506968 8_1:-55557882 None:intergenic
AGGAGTTATTATGGGGATCA+TGG 0.508047 8_1:+55559123 Msa1202090:three_prime_UTR
GATCTCTACGGTCCAGGAGT+TGG 0.516227 8_1:+55557633 Msa1202090:CDS
GCTGGTTTGTTCTCATTAGG+AGG 0.517479 8_1:+55557396 Msa1202090:CDS
ATTGGAGGACATTTGTTCAA+AGG 0.517913 8_1:+55558815 Msa1202090:CDS
AGAGCATTTGAACCATCTCT+AGG 0.524317 8_1:-55557954 None:intergenic
AGGTCTTCTTCTTCAGCTGA+AGG 0.529357 8_1:+55557741 Msa1202090:CDS
CATCTTGTTGTTGTTGCTGG+TGG 0.532790 8_1:-55557578 None:intergenic
TTTAGGTGTTGGACCAAGCA+GGG 0.533902 8_1:+55557656 Msa1202090:CDS
TCAAATTTACCTCTACTACT+TGG 0.536171 8_1:-55559227 None:intergenic
GTAAAAGAGAAGTATATATG+TGG 0.544134 8_1:-55559159 None:intergenic
GGGATAAGTTGTCAAGATTG+TGG 0.548229 8_1:+55557762 Msa1202090:CDS
ATAACTGTTGTTGACGCTCA+CGG 0.552876 8_1:-55557896 None:intergenic
CCAACACCTAAACCAACTCC+TGG 0.554565 8_1:-55557645 None:intergenic
CAAAATAGTGTCTCTTGTGG+TGG 0.555466 8_1:-55557468 None:intergenic
TAGAGATCTCGCGGGAAGAA+CGG 0.556385 8_1:-55557618 None:intergenic
TATCAAACGGCTGTTAACAT+TGG 0.556930 8_1:+55558797 Msa1202090:CDS
TGACCATGACATTGTTGTTG+TGG 0.557428 8_1:+55559204 Msa1202090:three_prime_UTR
TGATAGGTATGCATATCAAA+CGG 0.559733 8_1:+55558784 Msa1202090:CDS
AACAAACGTTGAAGGAAGGA+AGG 0.563270 8_1:+55556943 Msa1202090:five_prime_UTR
CAAACGGCTGTTAACATTGG+AGG 0.566332 8_1:+55558800 Msa1202090:CDS
ATTTCTAAACGCCCTAGAGA+TGG 0.568041 8_1:+55557942 Msa1202090:CDS
CGTACCACTAGCCGTCATGA+AGG 0.569674 8_1:-55559053 None:intergenic
TTCTTCCCGCGAGATCTCTA+CGG 0.572634 8_1:+55557621 Msa1202090:CDS
TCATCTGATGAAACCCTGCT+TGG 0.577114 8_1:-55557669 None:intergenic
AACTCATGTTAAAAGCACTT+GGG 0.578823 8_1:+55557857 Msa1202090:CDS
TAAAAGAGAAGTATATATGT+GGG 0.583599 8_1:-55559158 None:intergenic
CTTACACACCTGAACTCCGC+TGG 0.583863 8_1:-55558737 None:intergenic
TAATAGTAATTACATGATTG+GGG 0.584644 8_1:+55558901 Msa1202090:CDS
GGTGGTGGAAGAGGAAACCA+AGG 0.593423 8_1:+55557417 Msa1202090:CDS
GGTCTTCTTCTTCAGCTGAA+GGG 0.597588 8_1:+55557742 Msa1202090:CDS
AAGAAAGAGGAGTTATTATG+GGG 0.598652 8_1:+55559116 Msa1202090:three_prime_UTR
GAGCATTTGAACCATCTCTA+GGG 0.600057 8_1:-55557953 None:intergenic
AGCGGAGTTCAGGTGTGTAA+GGG 0.600996 8_1:+55558739 Msa1202090:CDS
TTGTTGTGGCCAAGTAGTAG+AGG 0.601864 8_1:+55559218 Msa1202090:three_prime_UTR
AGGATCAACAAGTGCGCCCG+AGG 0.604938 8_1:-55559002 None:intergenic
GGAGCCGAATACAATGAAGA+AGG 0.606433 8_1:-55559022 None:intergenic
AACGTTGAAGGAAGGAAGGA+AGG 0.609399 8_1:+55556947 Msa1202090:five_prime_UTR
GAGTCGGTGATTAGGTTCAA+CGG 0.611827 8_1:-55558959 None:intergenic
AATGCCTTCATGACGGCTAG+TGG 0.616126 8_1:+55559049 Msa1202090:CDS
ATCAACAAGTGCGCCCGAGG+AGG 0.617141 8_1:-55558999 None:intergenic
AACAACAGTGGTATCAGAGT+CGG 0.622301 8_1:-55558975 None:intergenic
AGAACTTGTTGTAAGAGTCG+TGG 0.626794 8_1:+55557822 Msa1202090:CDS
TCCGATTAATGCCTTCATGA+CGG 0.631102 8_1:+55559042 Msa1202090:CDS
GGACAGAAAACAGAAACAGA+CGG 0.636265 8_1:-55557050 None:intergenic
TGCTGTGGTAAGCTCACCAG+CGG 0.638941 8_1:+55558721 Msa1202090:CDS
GATGTTTCATCTTACAACAG+AGG 0.641152 8_1:+55557507 Msa1202090:CDS
CCTGGACCGTAGAGATCTCG+CGG 0.642572 8_1:-55557627 None:intergenic
TGGCCACAACAACAATGTCA+TGG 0.643734 8_1:-55559207 None:intergenic
CAGCGTTTACTATGGCTATG+AGG 0.650670 8_1:+55557721 Msa1202090:CDS
TACCAAAATAGTGTCTCTTG+TGG 0.654055 8_1:-55557471 None:intergenic
ATGATTGGGGAGACTTCCGG+TGG 0.658081 8_1:+55558914 Msa1202090:CDS
AAGATGATATGCATGCTGCA+CGG 0.670001 8_1:+55557595 Msa1202090:CDS
GCCCGAGGAGGCAACAACAG+TGG 0.675386 8_1:-55558987 None:intergenic
GACGCACACAAAAGAAAACA+CGG 0.689183 8_1:+55557187 Msa1202090:five_prime_UTR
CAAGTTCTACATCTCATGTG+AGG 0.701177 8_1:-55557807 None:intergenic
CAGACGGCTAAACAGTGTCG+TGG 0.701923 8_1:-55557034 None:intergenic
CTGGACCGTAGAGATCTCGC+GGG 0.715374 8_1:-55557626 None:intergenic
GGAGAAGAATCACAACAACA+AGG 0.732085 8_1:+55557438 Msa1202090:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTAATAATAATAATAA+AGG - chr8_1:55556850-55556869 None:intergenic 0.0%
!! TTAATAATAATAATAAAGGA+AGG - chr8_1:55556846-55556865 None:intergenic 10.0%
!!! TTTGTTTTGTTAAAAAATAA+AGG + chr8_1:55558677-55558696 Msa1202090:intron 10.0%
!!! TTGTTTTGTTAAAAAATAAA+GGG + chr8_1:55558678-55558697 Msa1202090:intron 10.0%
!!! AAGAATAAATATAGTATTTT+TGG - chr8_1:55559337-55559356 None:intergenic 10.0%
!! AATAATAGTAATTACATGAT+TGG + chr8_1:55558899-55558918 Msa1202090:CDS 15.0%
!! ATAATAGTAATTACATGATT+GGG + chr8_1:55558900-55558919 Msa1202090:CDS 15.0%
!! AATGAGATTATTATATATGA+AGG + chr8_1:55559275-55559294 Msa1202090:three_prime_UTR 15.0%
!! TATACAGAAAAACATAAGTT+AGG + chr8_1:55556907-55556926 Msa1202090:five_prime_UTR 20.0%
!! AATTGAAGTTAAGAAAAAGA+AGG - chr8_1:55557074-55557093 None:intergenic 20.0%
!!! ATTTGTGAAAAATGGTTTTT+GGG - chr8_1:55557216-55557235 None:intergenic 20.0%
!!! CTAAATAGGTTTTTTGTTTT+TGG - chr8_1:55557293-55557312 None:intergenic 20.0%
!! CAAAAACAAAAAACCTATTT+AGG + chr8_1:55557291-55557310 Msa1202090:five_prime_UTR 20.0%
!! TTAAAGAAACTACTGTTATT+AGG + chr8_1:55557343-55557362 Msa1202090:five_prime_UTR 20.0%
!! TAAAGAAACTACTGTTATTA+GGG + chr8_1:55557344-55557363 Msa1202090:five_prime_UTR 20.0%
!!! TTTTTTCTTCTTCAAAGAAA+TGG + chr8_1:55557374-55557393 Msa1202090:exon 20.0%
!! TTAGAAATATCTCTTTGTAA+AGG - chr8_1:55557930-55557949 None:intergenic 20.0%
!!! TTGCTTTTGTTTTTTAGTTA+AGG + chr8_1:55558035-55558054 Msa1202090:intron 20.0%
!!! TGTTTTTCACAAAAGAAAAA+GGG - chr8_1:55558388-55558407 None:intergenic 20.0%
!!! ATGTTTTTCACAAAAGAAAA+AGG - chr8_1:55558389-55558408 None:intergenic 20.0%
!! TAATAGTAATTACATGATTG+GGG + chr8_1:55558901-55558920 Msa1202090:CDS 20.0%
!!! AATTTTGTTTTCAAGATCTT+GGG - chr8_1:55559093-55559112 None:intergenic 20.0%
!!! TAAAAGAGAAGTATATATGT+GGG - chr8_1:55559161-55559180 None:intergenic 20.0%
!!! AATGATTGAAACACAAATTT+TGG - chr8_1:55559184-55559203 None:intergenic 20.0%
!! GATTTGTGAAAAATGGTTTT+TGG - chr8_1:55557217-55557236 None:intergenic 25.0%
! TAATTGGGATTTGTGAAAAA+TGG - chr8_1:55557224-55557243 None:intergenic 25.0%
!!! ACCTATTTAGGTTTTTGATA+TGG + chr8_1:55557303-55557322 Msa1202090:five_prime_UTR 25.0%
! TCCATATCAAAAACCTAAAT+AGG - chr8_1:55557307-55557326 None:intergenic 25.0%
!! ACAGAGGTAATTTAGAATTA+TGG + chr8_1:55557523-55557542 Msa1202090:CDS 25.0%
! ATTCATATAATAGTACCTGA+AGG - chr8_1:55557999-55558018 None:intergenic 25.0%
!!! TTTGTTTTTTAGTTAAGGAC+TGG + chr8_1:55558040-55558059 Msa1202090:intron 25.0%
!! AAAACAAAGAGAGAATCTTT+TGG + chr8_1:55558179-55558198 Msa1202090:intron 25.0%
! AAAACTATGTCATCAGTATT+TGG - chr8_1:55558232-55558251 None:intergenic 25.0%
!! AAATACTGATGACATAGTTT+TGG + chr8_1:55558231-55558250 Msa1202090:intron 25.0%
! CTGATAGATATGAAATATGA+TGG - chr8_1:55558320-55558339 None:intergenic 25.0%
! AAGAATCACAAAGAAAATGA+TGG - chr8_1:55558523-55558542 None:intergenic 25.0%
! GTTAAAAAATAAAGGGTTAG+AGG + chr8_1:55558685-55558704 Msa1202090:intron 25.0%
!! AAAGGAATTCTCTATGATTT+TGG + chr8_1:55558833-55558852 Msa1202090:CDS 25.0%
!!! CAATTTTGTTTTCAAGATCT+TGG - chr8_1:55559094-55559113 None:intergenic 25.0%
! AAACAAAATTGAGAAGAAAG+AGG + chr8_1:55559103-55559122 Msa1202090:three_prime_UTR 25.0%
!! GTAAAAGAGAAGTATATATG+TGG - chr8_1:55559162-55559181 None:intergenic 25.0%
! ATAGAAAGAAACAATAGCTT+TGG - chr8_1:55559380-55559399 None:intergenic 25.0%
! TTTCTATGTGTACTCTATAA+TGG + chr8_1:55559393-55559412 Msa1202090:three_prime_UTR 25.0%
TACACAAAAACAAACGTTGA+AGG + chr8_1:55556935-55556954 Msa1202090:five_prime_UTR 30.0%
AAGTATATGTCTCCTTGTTA+TGG - chr8_1:55557128-55557147 None:intergenic 30.0%
! CTGTGTTTTGTGTAATTTTC+TGG + chr8_1:55557163-55557182 Msa1202090:five_prime_UTR 30.0%
CAGTTGAGAGAGAATTAATT+GGG - chr8_1:55557239-55557258 None:intergenic 30.0%
ACAGTTGAGAGAGAATTAAT+TGG - chr8_1:55557240-55557259 None:intergenic 30.0%
AACTACTGTTATTAGGGTTA+AGG + chr8_1:55557350-55557369 Msa1202090:five_prime_UTR 30.0%
!! AAACTCATGTTAAAAGCACT+TGG + chr8_1:55557856-55557875 Msa1202090:CDS 30.0%
!! AACTCATGTTAAAAGCACTT+GGG + chr8_1:55557857-55557876 Msa1202090:CDS 30.0%
CTTAACCATAACACTGAAAA+GGG - chr8_1:55558131-55558150 None:intergenic 30.0%
TCTTAACCATAACACTGAAA+AGG - chr8_1:55558132-55558151 None:intergenic 30.0%
AGGAAAAACAGTTTCTTTCT+TGG - chr8_1:55558434-55558453 None:intergenic 30.0%
TGATAGGTATGCATATCAAA+CGG + chr8_1:55558784-55558803 Msa1202090:CDS 30.0%
TTATTGTTGGTACTACTTTC+TGG - chr8_1:55558860-55558879 None:intergenic 30.0%
! ACTATTGTTGCTGTTATTGT+TGG - chr8_1:55558873-55558892 None:intergenic 30.0%
TGATCCTTCTTCATTGTATT+CGG + chr8_1:55559018-55559037 Msa1202090:CDS 30.0%
AGAAGAAAGAGGAGTTATTA+TGG + chr8_1:55559114-55559133 Msa1202090:three_prime_UTR 30.0%
GAAGAAAGAGGAGTTATTAT+GGG + chr8_1:55559115-55559134 Msa1202090:three_prime_UTR 30.0%
AAGAAAGAGGAGTTATTATG+GGG + chr8_1:55559116-55559135 Msa1202090:three_prime_UTR 30.0%
TCAAATTTACCTCTACTACT+TGG - chr8_1:55559230-55559249 None:intergenic 30.0%
!! TACTCTATAATGGACTTCTA+TGG + chr8_1:55559403-55559422 Msa1202090:three_prime_UTR 30.0%
CAAAAACAAACGTTGAAGGA+AGG + chr8_1:55556939-55556958 Msa1202090:five_prime_UTR 35.0%
! TATGTCTCCTTGTTATGGTT+GGG - chr8_1:55557123-55557142 None:intergenic 35.0%
! ATATGTCTCCTTGTTATGGT+TGG - chr8_1:55557124-55557143 None:intergenic 35.0%
TTCTTCTTCAAAGAAATGGC+TGG + chr8_1:55557378-55557397 Msa1202090:exon 35.0%
TACCAAAATAGTGTCTCTTG+TGG - chr8_1:55557474-55557493 None:intergenic 35.0%
GATGTTTCATCTTACAACAG+AGG + chr8_1:55557507-55557526 Msa1202090:CDS 35.0%
!! TATCATCTTGTTGTTGTTGC+TGG - chr8_1:55557584-55557603 None:intergenic 35.0%
! TCAACTCGTAATTTTCCTTC+AGG + chr8_1:55557981-55558000 Msa1202090:CDS 35.0%
TACTGTGACATAAGGTGTAA+GGG + chr8_1:55558068-55558087 Msa1202090:intron 35.0%
! ATTGACCCTTTTCAGTGTTA+TGG + chr8_1:55558123-55558142 Msa1202090:intron 35.0%
!!! GAGAGAATCTTTTGGTTTAG+AGG + chr8_1:55558187-55558206 Msa1202090:intron 35.0%
ATAAACACACGCAATCGATA+CGG - chr8_1:55558280-55558299 None:intergenic 35.0%
AACAGTTTCTTTCTTGGTCA+GGG - chr8_1:55558428-55558447 None:intergenic 35.0%
AAACAGTTTCTTTCTTGGTC+AGG - chr8_1:55558429-55558448 None:intergenic 35.0%
!! CAATTGATGATGCAGATGAT+AGG + chr8_1:55558768-55558787 Msa1202090:CDS 35.0%
TATCAAACGGCTGTTAACAT+TGG + chr8_1:55558797-55558816 Msa1202090:CDS 35.0%
ATTGGAGGACATTTGTTCAA+AGG + chr8_1:55558815-55558834 Msa1202090:CDS 35.0%
AACAAACGTTGAAGGAAGGA+AGG + chr8_1:55556943-55556962 Msa1202090:five_prime_UTR 40.0%
GGACAGAAAACAGAAACAGA+CGG - chr8_1:55557053-55557072 None:intergenic 40.0%
! CTCTTTTCCCAACCATAACA+AGG + chr8_1:55557113-55557132 Msa1202090:five_prime_UTR 40.0%
GACGCACACAAAAGAAAACA+CGG + chr8_1:55557187-55557206 Msa1202090:five_prime_UTR 40.0%
!! ATGGCTGGTTTGTTCTCATT+AGG + chr8_1:55557393-55557412 Msa1202090:CDS 40.0%
GTTGTTGTGATTCTTCTCCT+TGG - chr8_1:55557437-55557456 None:intergenic 40.0%
GGAGAAGAATCACAACAACA+AGG + chr8_1:55557438-55557457 Msa1202090:CDS 40.0%
CAAAATAGTGTCTCTTGTGG+TGG - chr8_1:55557471-55557490 None:intergenic 40.0%
! CACCACAAGAGACACTATTT+TGG + chr8_1:55557469-55557488 Msa1202090:CDS 40.0%
AAGATGATATGCATGCTGCA+CGG + chr8_1:55557595-55557614 Msa1202090:CDS 40.0%
GGGATAAGTTGTCAAGATTG+TGG + chr8_1:55557762-55557781 Msa1202090:CDS 40.0%
GAGGACAATCTTTCTTAGCT+TGG - chr8_1:55557791-55557810 None:intergenic 40.0%
CAAGTTCTACATCTCATGTG+AGG - chr8_1:55557810-55557829 None:intergenic 40.0%
!! AGAACTTGTTGTAAGAGTCG+TGG + chr8_1:55557822-55557841 Msa1202090:CDS 40.0%
ATAACTGTTGTTGACGCTCA+CGG - chr8_1:55557899-55557918 None:intergenic 40.0%
ATTTCTAAACGCCCTAGAGA+TGG + chr8_1:55557942-55557961 Msa1202090:CDS 40.0%
GAGCATTTGAACCATCTCTA+GGG - chr8_1:55557956-55557975 None:intergenic 40.0%
! AGAGCATTTGAACCATCTCT+AGG - chr8_1:55557957-55557976 None:intergenic 40.0%
TGGACTTGTACTGTGACATA+AGG + chr8_1:55558060-55558079 Msa1202090:intron 40.0%
GTACTGTGACATAAGGTGTA+AGG + chr8_1:55558067-55558086 Msa1202090:intron 40.0%
ACACTGAAAAGGGTCAATCA+CGG - chr8_1:55558121-55558140 None:intergenic 40.0%
GTTATGGTTAAGAGAGTGAG+TGG + chr8_1:55558139-55558158 Msa1202090:intron 40.0%
GAGAGTGAGTGGTGAAATAA+AGG + chr8_1:55558150-55558169 Msa1202090:intron 40.0%
GATTGAGGTGAGAATATGTG+TGG - chr8_1:55558492-55558511 None:intergenic 40.0%
ATGATGGTAGCTAGTGATTG+AGG - chr8_1:55558507-55558526 None:intergenic 40.0%
AACAACAGTGGTATCAGAGT+CGG - chr8_1:55558978-55558997 None:intergenic 40.0%
TCCGATTAATGCCTTCATGA+CGG + chr8_1:55559042-55559061 Msa1202090:CDS 40.0%
AGGAGTTATTATGGGGATCA+TGG + chr8_1:55559123-55559142 Msa1202090:three_prime_UTR 40.0%
! TGACCATGACATTGTTGTTG+TGG + chr8_1:55559204-55559223 Msa1202090:three_prime_UTR 40.0%
AACGTTGAAGGAAGGAAGGA+AGG + chr8_1:55556947-55556966 Msa1202090:five_prime_UTR 45.0%
TTGAAGGAAGGAAGGAAGGA+AGG + chr8_1:55556951-55556970 Msa1202090:five_prime_UTR 45.0%
!! GCTGGTTTGTTCTCATTAGG+AGG + chr8_1:55557396-55557415 Msa1202090:CDS 45.0%
! GGTTTGTTCTCATTAGGAGG+TGG + chr8_1:55557399-55557418 Msa1202090:CDS 45.0%
TTGTTCTCATTAGGAGGTGG+TGG + chr8_1:55557402-55557421 Msa1202090:CDS 45.0%
!! GTTGTTGGTGCTGTTGTTGT+TGG - chr8_1:55557557-55557576 None:intergenic 45.0%
!! CATCTTGTTGTTGTTGCTGG+TGG - chr8_1:55557581-55557600 None:intergenic 45.0%
!! TTTAGGTGTTGGACCAAGCA+GGG + chr8_1:55557656-55557675 Msa1202090:CDS 45.0%
TCATCTGATGAAACCCTGCT+TGG - chr8_1:55557672-55557691 None:intergenic 45.0%
ACATCAATCGTCGTCGAGAT+CGG + chr8_1:55557698-55557717 Msa1202090:CDS 45.0%
CAGCGTTTACTATGGCTATG+AGG + chr8_1:55557721-55557740 Msa1202090:CDS 45.0%
AGGTCTTCTTCTTCAGCTGA+AGG + chr8_1:55557741-55557760 Msa1202090:CDS 45.0%
GGTCTTCTTCTTCAGCTGAA+GGG + chr8_1:55557742-55557761 Msa1202090:CDS 45.0%
GGAATGTGCTAAGAAAGAGG+AGG - chr8_1:55558454-55558473 None:intergenic 45.0%
GAGGGAATGTGCTAAGAAAG+AGG - chr8_1:55558457-55558476 None:intergenic 45.0%
GTGTGGATGTGAAAGAGAGA+GGG - chr8_1:55558475-55558494 None:intergenic 45.0%
TGTGTGGATGTGAAAGAGAG+AGG - chr8_1:55558476-55558495 None:intergenic 45.0%
! GGAAGCAAATTTTCCTGCTG+TGG + chr8_1:55558706-55558725 Msa1202090:CDS 45.0%
CAAACGGCTGTTAACATTGG+AGG + chr8_1:55558800-55558819 Msa1202090:CDS 45.0%
TACATGATTGGGGAGACTTC+CGG + chr8_1:55558911-55558930 Msa1202090:CDS 45.0%
GAGTCGGTGATTAGGTTCAA+CGG - chr8_1:55558962-55558981 None:intergenic 45.0%
TGGTATCAGAGTCGGTGATT+AGG - chr8_1:55558970-55558989 None:intergenic 45.0%
GGAGCCGAATACAATGAAGA+AGG - chr8_1:55559025-55559044 None:intergenic 45.0%
!! GCCGTCATGAAGGCATTAAT+CGG - chr8_1:55559046-55559065 None:intergenic 45.0%
!! GTTTTCAAGATCTTGGGCGA+GGG - chr8_1:55559087-55559106 None:intergenic 45.0%
!! TGTTTTCAAGATCTTGGGCG+AGG - chr8_1:55559088-55559107 None:intergenic 45.0%
TGGCCACAACAACAATGTCA+TGG - chr8_1:55559210-55559229 None:intergenic 45.0%
TTGTTGTGGCCAAGTAGTAG+AGG + chr8_1:55559218-55559237 Msa1202090:three_prime_UTR 45.0%
AAGCTATAGCTGCTGCTAGC+TGG + chr8_1:55556974-55556993 Msa1202090:five_prime_UTR 50.0%
AGCTATAGCTGCTGCTAGCT+GGG + chr8_1:55556975-55556994 Msa1202090:five_prime_UTR 50.0%
!! TCATTAGGAGGTGGTGGAAG+AGG + chr8_1:55557408-55557427 Msa1202090:CDS 50.0%
!! GGTGCTGTTGTTGTTGGTGT+TGG - chr8_1:55557551-55557570 None:intergenic 50.0%
!! GTTGTTGCTGGTGGTGTTGT+TGG - chr8_1:55557572-55557591 None:intergenic 50.0%
TAGAGATCTCGCGGGAAGAA+CGG - chr8_1:55557621-55557640 None:intergenic 50.0%
TTCTTCCCGCGAGATCTCTA+CGG + chr8_1:55557621-55557640 Msa1202090:CDS 50.0%
! TACGGTCCAGGAGTTGGTTT+AGG + chr8_1:55557639-55557658 Msa1202090:CDS 50.0%
CCAACACCTAAACCAACTCC+TGG - chr8_1:55557648-55557667 None:intergenic 50.0%
! CCAGGAGTTGGTTTAGGTGT+TGG + chr8_1:55557645-55557664 Msa1202090:CDS 50.0%
!! GTTTAGGTGTTGGACCAAGC+AGG + chr8_1:55557655-55557674 Msa1202090:CDS 50.0%
GAGATCGGCAGCGTTTACTA+TGG + chr8_1:55557713-55557732 Msa1202090:CDS 50.0%
ACACACGCAATCGATACGGT+TGG - chr8_1:55558276-55558295 None:intergenic 50.0%
AGCGGAGTTCAGGTGTGTAA+GGG + chr8_1:55558739-55558758 Msa1202090:CDS 50.0%
TACCACTGTTGTTGCCTCCT+CGG + chr8_1:55558985-55559004 Msa1202090:CDS 50.0%
AATGCCTTCATGACGGCTAG+TGG + chr8_1:55559049-55559068 Msa1202090:CDS 50.0%
CAGACGGCTAAACAGTGTCG+TGG - chr8_1:55557037-55557056 None:intergenic 55.0%
!! GGTGGTGGAAGAGGAAACCA+AGG + chr8_1:55557417-55557436 Msa1202090:CDS 55.0%
GATCTCTACGGTCCAGGAGT+TGG + chr8_1:55557633-55557652 Msa1202090:CDS 55.0%
TGCTGTGGTAAGCTCACCAG+CGG + chr8_1:55558721-55558740 Msa1202090:CDS 55.0%
TAAGCTCACCAGCGGAGTTC+AGG + chr8_1:55558729-55558748 Msa1202090:CDS 55.0%
CTTACACACCTGAACTCCGC+TGG - chr8_1:55558740-55558759 None:intergenic 55.0%
CAGCGGAGTTCAGGTGTGTA+AGG + chr8_1:55558738-55558757 Msa1202090:CDS 55.0%
ATGATTGGGGAGACTTCCGG+TGG + chr8_1:55558914-55558933 Msa1202090:CDS 55.0%
ACCACTGTTGTTGCCTCCTC+GGG + chr8_1:55558986-55559005 Msa1202090:CDS 55.0%
CGTACCACTAGCCGTCATGA+AGG - chr8_1:55559056-55559075 None:intergenic 55.0%
CTGGACCGTAGAGATCTCGC+GGG - chr8_1:55557629-55557648 None:intergenic 60.0%
CCTGGACCGTAGAGATCTCG+CGG - chr8_1:55557630-55557649 None:intergenic 60.0%
GCTGGTGAGCTTACCACAGC+AGG - chr8_1:55558722-55558741 None:intergenic 60.0%
ATTGGGGAGACTTCCGGTGG+AGG + chr8_1:55558917-55558936 Msa1202090:CDS 60.0%
ATCAACAAGTGCGCCCGAGG+AGG - chr8_1:55559002-55559021 None:intergenic 60.0%
AGGATCAACAAGTGCGCCCG+AGG - chr8_1:55559005-55559024 None:intergenic 60.0%
CCGCGAGATCTCTACGGTCC+AGG + chr8_1:55557627-55557646 Msa1202090:CDS 65.0%
CGCTCACGGCGTCTAGAAGC+AGG - chr8_1:55557885-55557904 None:intergenic 65.0%
ACTTCCGGTGGAGGCGGTGT+TGG + chr8_1:55558926-55558945 Msa1202090:CDS 65.0%
AACGCCAACACCGCCTCCAC+CGG - chr8_1:55558933-55558952 None:intergenic 65.0%
GCCCGAGGAGGCAACAACAG+TGG - chr8_1:55558990-55559009 None:intergenic 65.0%
GGGGAGACTTCCGGTGGAGG+CGG + chr8_1:55558920-55558939 Msa1202090:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 55556833 55559450 55556833 ID=Msa1202090;Name=Msa1202090
chr8_1 mRNA 55556833 55559450 55556833 ID=Msa1202090-mRNA-1;Parent=Msa1202090;Name=Msa1202090-mRNA-1;_AED=0.00;_eAED=0.00;_QI=560|1|1|1|1|1|2|348|337
chr8_1 exon 55556833 55558002 55556833 ID=Msa1202090-mRNA-1:exon:17973;Parent=Msa1202090-mRNA-1
chr8_1 exon 55558699 55559450 55558699 ID=Msa1202090-mRNA-1:exon:17974;Parent=Msa1202090-mRNA-1
chr8_1 five_prime_UTR 55556833 55557392 55556833 ID=Msa1202090-mRNA-1:five_prime_utr;Parent=Msa1202090-mRNA-1
chr8_1 CDS 55557393 55558002 55557393 ID=Msa1202090-mRNA-1:cds;Parent=Msa1202090-mRNA-1
chr8_1 CDS 55558699 55559102 55558699 ID=Msa1202090-mRNA-1:cds;Parent=Msa1202090-mRNA-1
chr8_1 three_prime_UTR 55559103 55559450 55559103 ID=Msa1202090-mRNA-1:three_prime_utr;Parent=Msa1202090-mRNA-1
Gene Sequence

>Msa1202090

ATGGCTGGTTTGTTCTCATTAGGAGGTGGTGGAAGAGGAAACCAAGGAGAAGAATCACAACAACAAGGTCATATTCCACCACAAGAGACACTATTTTGGTACAACAAAAACGATGATGTTTCATCTTACAACAGAGGTAATTTAGAATTATGGAACCAACACCAACAACAACAGCACCAACAACACCACCAGCAACAACAACAAGATGATATGCATGCTGCACGGCCGTTCTTCCCGCGAGATCTCTACGGTCCAGGAGTTGGTTTAGGTGTTGGACCAAGCAGGGTTTCATCAGATGATCATGAACATCAATCGTCGTCGAGATCGGCAGCGTTTACTATGGCTATGAGGTCTTCTTCTTCAGCTGAAGGGATAAGTTGTCAAGATTGTGGTAACCAAGCTAAGAAAGATTGTCCTCACATGAGATGTAGAACTTGTTGTAAGAGTCGTGGCTTTCAGTGTCAAACTCATGTTAAAAGCACTTGGGTTCCTGCTTCTAGACGCCGTGAGCGTCAACAACAGTTATCTTCTTCTCCTTTACAAAGAGATATTTCTAAACGCCCTAGAGATGGTTCAAATGCTCTTGTTTCAACTCGTAATTTTCCTTCAGGGTTAGAGGAAGCAAATTTTCCTGCTGTGGTAAGCTCACCAGCGGAGTTCAGGTGTGTAAGGGTTAGTTCAATTGATGATGCAGATGATAGGTATGCATATCAAACGGCTGTTAACATTGGAGGACATTTGTTCAAAGGAATTCTCTATGATTTTGGTCCAGAAAGTAGTACCAACAATAACAGCAACAATAGTAATTATAATAATAGTAATTACATGATTGGGGAGACTTCCGGTGGAGGCGGTGTTGGCGTTGCTCAACCGTTGAACCTAATCACCGACTCTGATACCACTGTTGTTGCCTCCTCGGGCGCACTTGTTGATCCTTCTTCATTGTATTCGGCTCCGATTAATGCCTTCATGACGGCTAGTGGTACGCAATTTTTCCCTCGCCCAAGATCTTGA
Protein sequence

>Msa1202090

MAGLFSLGGGGRGNQGEESQQQGHIPPQETLFWYNKNDDVSSYNRGNLELWNQHQQQQHQQHHQQQQQDDMHAARPFFPRDLYGPGVGLGVGPSRVSSDDHEHQSSSRSAAFTMAMRSSSSAEGISCQDCGNQAKKDCPHMRCRTCCKSRGFQCQTHVKSTWVPASRRRERQQQLSSSPLQRDISKRPRDGSNALVSTRNFPSGLEEANFPAVVSSPAEFRCVRVSSIDDADDRYAYQTAVNIGGHLFKGILYDFGPESSTNNNSNNSNYNNSNYMIGETSGGGGVGVAQPLNLITDSDTTVVASSGALVDPSSLYSAPINAFMTASGTQFFPRPRS*