AlfalfaGEDB | Msa1203080

M. sativa cultivar zhongmu-4 / Msa1203080

KEGG: -
GO: GO:0000003 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009791 GO:0009889 GO:0009937 GO:0009966 GO:0010029 GO:0010154 GO:0010431 GO:0010468 GO:0010556 GO:0010646 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0023051 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0048316 GO:0048580 GO:0048583 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071695 GO:0080090 GO:0097159 GO:0140110 GO:1900140 GO:1901363 GO:1903506 GO:2000026 GO:2000112 GO:2001141
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1203080	sp\|Q9SCQ6\|IDD2_ARATH	82.558	86	13	2	2	86	3	87	7.94e-39	146
Msa1203080	sp\|Q9SCQ6\|IDD2_ARATH	42.751	269	88	10	89	355	245	449	1.82e-36	140

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1203080	A0A396GNR0	100.000	86	0	0	1	86	1	86	8.01e-51	187
Msa1203080	A0A396GNR0	97.080	274	8	0	84	357	246	519	0.0	522

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TFs/TRs:

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Gene ID	Type	Classification
Msa1203080	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1203080	MtrunA17_Chr8g0373541	97.080	274	8	0	84	357	246	519	0.0	522
Msa1203080	MtrunA17_Chr8g0373541	100.000	86	0	0	1	86	1	86	1.54e-54	187
Msa1203080	MtrunA17_Chr2g0330171	60.606	99	23	1	4	86	1	99	1.15e-31	124
Msa1203080	MtrunA17_Chr8g0342421	60.606	99	24	2	3	86	17	115	3.54e-31	123
Msa1203080	MtrunA17_Chr1g0185121	65.909	88	25	1	4	86	6	93	1.15e-30	121
Msa1203080	MtrunA17_Chr7g0263941	87.931	58	7	0	29	86	30	87	3.87e-30	119
Msa1203080	MtrunA17_Chr4g0029521	66.250	80	23	1	11	86	12	91	4.07e-30	120
Msa1203080	MtrunA17_Chr3g0141431	86.207	58	8	0	29	86	18	75	8.72e-30	118
Msa1203080	MtrunA17_Chr3g0141431	42.149	121	57	4	211	331	300	407	3.72e-14	73.2
Msa1203080	MtrunA17_Chr1g0181381	80.303	66	8	1	26	86	26	91	1.10e-29	119

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1203080	AT3G50700.1	82.558	86	13	2	2	86	3	87	8.08e-40	146
Msa1203080	AT3G50700.1	42.751	269	88	10	89	355	245	449	1.85e-37	140
Msa1203080	AT5G66730.1	46.570	277	101	10	90	357	262	500	1.41e-39	147
Msa1203080	AT5G66730.1	80.233	86	13	2	2	86	3	85	8.03e-39	144
Msa1203080	AT4G02670.2	57.944	107	34	3	8	103	21	127	3.03e-29	117
Msa1203080	AT4G02670.1	57.944	107	34	3	8	103	19	125	3.26e-29	117
Msa1203080	AT5G44160.1	70.588	85	23	2	4	86	6	90	1.79e-28	115
Msa1203080	AT3G13810.5	46.970	132	47	2	3	111	14	145	1.86e-28	116
Msa1203080	AT3G13810.4	46.970	132	47	2	3	111	19	150	1.99e-28	115
Msa1203080	AT3G13810.1	46.970	132	47	2	3	111	19	150	1.99e-28	115

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Find 142 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAAGCGTTGTCTGCTT+AGG	0.462981	8_1:-56863568	None:intergenic
AAATCCACCCCAAATCATAG+AGG	0.606558	8_1:+56862943	Msa1203080:CDS
AAATTAAGGGTATGTGAATA+TGG	0.414970	8_1:+56864058	Msa1203080:three_prime_UTR
AACGCTTGATTTCCTAGGAT+TGG	0.393689	8_1:+56863579	Msa1203080:CDS
AACTACTAGTGTTATTATCA+GGG	0.565154	8_1:+56863766	Msa1203080:three_prime_UTR
AAGAAAATGTGACCTTAACA+AGG	0.633723	8_1:+56863999	Msa1203080:three_prime_UTR
AAGCACATTGTACCTTGTTA+AGG	0.439079	8_1:-56864011	None:intergenic
AAGCTACCTAAAGAGGGAAT+AGG	0.450917	8_1:-56864388	None:intergenic
AAGGCAAAGAGAGATCGCCT+CGG	0.703738	8_1:-56863206	None:intergenic
AAGGTACAATGTGCTTGTTT+TGG	0.357051	8_1:+56864018	Msa1203080:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATTGTCTAAATCAACCATT+GGG	-	chr8_1:56861962-56861981	None:intergenic	30.0%
ACATTGTCTAAATCAACCAT+TGG	-	chr8_1:56861963-56861982	None:intergenic	30.0%
AAGAAAAAAAGAAATCTCCC+AGG	+	chr8_1:56862059-56862078	Msa1203080:CDS	30.0%
GTGTGTGAAATATGTAACAA+AGG	+	chr8_1:56862235-56862254	Msa1203080:CDS	30.0%
AATCTTCAACTACATAGAAG+AGG	+	chr8_1:56862274-56862293	Msa1203080:CDS	30.0%
ATTGTTTGTTTATGTGGTCT+TGG	+	chr8_1:56862488-56862507	Msa1203080:intron	30.0%
AACACATAGACAAGTAAAGT+TGG	-	chr8_1:56862788-56862807	None:intergenic	30.0%
CATAGTTGCATATTGCATAT+TGG	-	chr8_1:56863890-56863909	None:intergenic	30.0%
CAATATGCAATATGCAACTA+TGG	+	chr8_1:56863888-56863907	Msa1203080:three_prime_UTR	30.0%
AAGAAAATGTGACCTTAACA+AGG	+	chr8_1:56863999-56864018	Msa1203080:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	56861749	56864472	56861749	ID=Msa1203080;Name=Msa1203080
chr8_1	mRNA	56861749	56864472	56861749	ID=Msa1203080-mRNA-1;Parent=Msa1203080;Name=Msa1203080-mRNA-1;_AED=0.01;_eAED=0.01;_QI=214\|0.5\|0.66\|1\|1\|1\|3\|725\|357
chr8_1	exon	56861749	56862089	56861749	ID=Msa1203080-mRNA-1:exon:18552;Parent=Msa1203080-mRNA-1
chr8_1	exon	56862176	56862307	56862176	ID=Msa1203080-mRNA-1:exon:18553;Parent=Msa1203080-mRNA-1
chr8_1	exon	56862933	56864472	56862933	ID=Msa1203080-mRNA-1:exon:18554;Parent=Msa1203080-mRNA-1
chr8_1	five_prime_UTR	56861749	56861962	56861749	ID=Msa1203080-mRNA-1:five_prime_utr;Parent=Msa1203080-mRNA-1
chr8_1	CDS	56861963	56862089	56861963	ID=Msa1203080-mRNA-1:cds;Parent=Msa1203080-mRNA-1
chr8_1	CDS	56862176	56862307	56862176	ID=Msa1203080-mRNA-1:cds;Parent=Msa1203080-mRNA-1
chr8_1	CDS	56862933	56863747	56862933	ID=Msa1203080-mRNA-1:cds;Parent=Msa1203080-mRNA-1
chr8_1	three_prime_UTR	56863748	56864472	56863748	ID=Msa1203080-mRNA-1:three_prime_utr;Parent=Msa1203080-mRNA-1

Gene Sequence

>Msa1203080

ATGGTTGATTTAGACAATGTTTCCACAGCTTCAGGTGAAGCTAGCATCTCTTCCTCTGGTAATAACAATATTCAATCACCAATTCCAAAACCCACCAAGAAAAAAAGAAATCTCCCAGGAATGCCAGATCCAGAAGCAGAGGTGATTGCTCTGTCACCAACAACGTTGTTAGCTACAAACAGATTTGTGTGTGAAATATGTAACAAAGGTTTTCAAAGAGATCAGAATCTTCAACTACATAGAAGAGGACATAATCTAGATTTGCCAGAAAATCCACCCCAAATCATAGAGGAACCACAAGTTGTTGTTACTACTACTGCTAGTGCTTTAAATGCAAGTTGTAGCAGCAGTACTAGCTCAACAAGCAACGGCTGTGCTACTACCAGCACTGGTGTGTTTGCGAGCTTGTTTGCTTCATCAACAGCTTCAGCTTCTGCGAGTCTCCAACCTCAGGCACCGGCGTTCAGTGACCTAATTAGGTCCATGGGATGTACAGATCCCCGTCCTACAAATTTCTCAGCTCCTCCATCGTCCGAGGCGATCTCTCTTTGCCTTTCGACCAATCATGGATCATCAATTTTCGGAACAGGAGGGCAGGAGTGTCGCCAATACGTGCCAACACATCAGCCGCCCGCAATGTCTGCTACTGCATTACTTCAAAAAGCAGCTCAAATGGGCGCTGCTGCAACAAACGCCTCTCTGCTTCGCGGGCTTGGAATTGTGTCGTCTTCTGCTTCCACTTCATCTGGCCAACAAGATAGCCTGCATTGGGGTCTCGGACAAATGGAACCAGAGGGTTCTAGTTTAGTTTCAGCAGGACTTGGACTAGGTCTTCCTTGTGACGGTGATTCTGGACTAAAGGAATTGATGTTGGGGACTCCGTCAATGTTTGGTCCTAAGCAGACAACGCTTGATTTCCTAGGATTGGGGATGGCTGCTGGTGGCAGTGCCGGTGGAGGCCTGTCTGCACTTATCACATCAATTGGCGGAGGCAGTGGTTTGGATGTTACCGCAGCAGCCGCATCCTTCGGTAATGGAGAGTTCTCTGGCAAAGATATTGGAAGAAGTTCATGA

Protein sequence

>Msa1203080

MVDLDNVSTASGEASISSSGNNNIQSPIPKPTKKKRNLPGMPDPEAEVIALSPTTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLDLPENPPQIIEEPQVVVTTTASALNASCSSSTSSTSNGCATTSTGVFASLFASSTASASASLQPQAPAFSDLIRSMGCTDPRPTNFSAPPSSEAISLCLSTNHGSSIFGTGGQECRQYVPTHQPPAMSATALLQKAAQMGAAATNASLLRGLGIVSSSASTSSGQQDSLHWGLGQMEPEGSSLVSAGLGLGLPCDGDSGLKELMLGTPSMFGPKQTTLDFLGLGMAAGGSAGGGLSALITSIGGGSGLDVTAAAASFGNGEFSGKDIGRSS*