AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1204230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204230 MtrunA17_Chr4g0063371 82.051 117 14 1 1 117 116 225 1.52e-56 175
Msa1204230 MtrunA17_Chr4g0063391 52.688 93 14 1 1 63 118 210 7.39e-26 96.7
Msa1204230 MtrunA17_Chr4g0063401 41.748 103 18 2 1 62 147 248 1.17e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204230 AT3G19360.1 29.508 183 62 4 1 117 186 367 9.09e-18 77.4
Msa1204230 AT3G19360.2 29.508 183 62 4 1 117 186 367 9.09e-18 77.4

Find 36 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAGATCCTGCACAATTT+AGG 0.163729 8_1:+59743052 Msa1204230:CDS
CTCCCCATTGCTATACTTCT+TGG 0.165677 8_1:-59742995 None:intergenic
CCAAGTATACGTTTCTTCTT+AGG 0.214369 8_1:-59744049 None:intergenic
TGAAGATCCTGCACAATTTA+GGG 0.278155 8_1:+59743053 Msa1204230:CDS
AGTCAGATCAATCGTATTTA+CGG 0.310380 8_1:+59744082 Msa1204230:CDS
GATACAGGATATTGTTCCTT+TGG 0.336419 8_1:+59743114 Msa1204230:CDS
CACAATTTAGGGGCACTTAT+CGG 0.370314 8_1:+59743064 Msa1204230:CDS
AATTGCAGCTTCAGCTTCAA+TGG 0.390999 8_1:-59743957 None:intergenic
AAATCAGAGTTACAAGTTCC+TGG 0.400098 8_1:+59743929 Msa1204230:intron
GACTTGCAGGGGAGGTGGTA+GGG 0.419543 8_1:-59744000 None:intergenic
TGCAAGTCAACTTGTGGAAA+AGG 0.437938 8_1:+59744015 Msa1204230:CDS
CCTAAGAAGAAACGTATACT+TGG 0.441257 8_1:+59744049 Msa1204230:CDS
TGTCCAAGAAGTATAGCAAT+GGG 0.457675 8_1:+59742992 Msa1204230:CDS
CACTTTGCTCATGGTGAAGA+AGG 0.463092 8_1:+59743147 Msa1204230:CDS
TGTCTCAAGTGGAAGGATAC+AGG 0.464303 8_1:+59743099 Msa1204230:CDS
TGACTTGCAGGGGAGGTGGT+AGG 0.490726 8_1:-59744001 None:intergenic
CACAAGTTGACTTGCAGGGG+AGG 0.509364 8_1:-59744008 None:intergenic
AATTTACACTTATCTGCATA+GGG 0.538027 8_1:-59743024 None:intergenic
CTCCCCTGCAAGTCAACTTG+TGG 0.543090 8_1:+59744009 Msa1204230:CDS
AAGTTGACTTGCAGGGGAGG+TGG 0.548779 8_1:-59744005 None:intergenic
CTGTCCAAGAAGTATAGCAA+TGG 0.557285 8_1:+59742991 Msa1204230:CDS
GAATTTACACTTATCTGCAT+AGG 0.559420 8_1:-59743025 None:intergenic
TTTCCACAAGTTGACTTGCA+GGG 0.560920 8_1:-59744012 None:intergenic
TAAGTGCCCCTAAATTGTGC+AGG 0.568865 8_1:-59743060 None:intergenic
TCAGCTTCAATGGCTCCACC+AGG 0.575223 8_1:-59743947 None:intergenic
CTAAGAAGAAACGTATACTT+GGG 0.583825 8_1:+59744050 Msa1204230:CDS
AAGAACTGTCACTTTGCTCA+TGG 0.585017 8_1:+59743138 Msa1204230:CDS
TTCCACAAGTTGACTTGCAG+GGG 0.588906 8_1:-59744011 None:intergenic
AGACTAAGCTGTGTCTCAAG+TGG 0.589149 8_1:+59743088 Msa1204230:CDS
ATTGTGCAGGATCTTCATGA+AGG 0.599081 8_1:-59743047 None:intergenic
TAAGCTGTGTCTCAAGTGGA+AGG 0.609632 8_1:+59743092 Msa1204230:CDS
AAAGTGACAGTTCTTTCCAA+AGG 0.613225 8_1:-59743130 None:intergenic
GAAGATCCTGCACAATTTAG+GGG 0.650410 8_1:+59743054 Msa1204230:CDS
CAAGAAGTATAGCAATGGGG+AGG 0.682965 8_1:+59742996 Msa1204230:CDS
TCAGAGTTACAAGTTCCTGG+TGG 0.718686 8_1:+59743932 Msa1204230:intron
GTCCAAGAAGTATAGCAATG+GGG 0.754417 8_1:+59742993 Msa1204230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAAAGTTTTACATT+TGG + chr8_1:59743460-59743479 Msa1204230:intron 10.0%
!!! GACAAATTTTTTTAAATATA+AGG - chr8_1:59743781-59743800 None:intergenic 10.0%
!!! AAAAAAAAAAGTTTTACATT+TGG + chr8_1:59743460-59743479 Msa1204230:intron 10.0%
!!! GACAAATTTTTTTAAATATA+AGG - chr8_1:59743781-59743800 None:intergenic 10.0%
!!! TTTTTTTAAATATAAGGTCT+AGG - chr8_1:59743775-59743794 None:intergenic 15.0%
!!! TTTTTTTAAATATAAGGTCT+AGG - chr8_1:59743775-59743794 None:intergenic 15.0%
!!! CAATAACTCATTTTGATTAT+TGG + chr8_1:59743173-59743192 Msa1204230:intron 20.0%
!!! AAGATACTATAATTTCTAGT+AGG + chr8_1:59743484-59743503 Msa1204230:intron 20.0%
!!! TTATAACAATTGCAACTTTT+TGG + chr8_1:59743861-59743880 Msa1204230:intron 20.0%
!!! CAATAACTCATTTTGATTAT+TGG + chr8_1:59743173-59743192 Msa1204230:intron 20.0%
!!! AAGATACTATAATTTCTAGT+AGG + chr8_1:59743484-59743503 Msa1204230:intron 20.0%
!!! TTATAACAATTGCAACTTTT+TGG + chr8_1:59743861-59743880 Msa1204230:intron 20.0%
! AATTTACACTTATCTGCATA+GGG - chr8_1:59743027-59743046 None:intergenic 25.0%
!!! CTCATTTTGATTATTGGTTT+TGG + chr8_1:59743179-59743198 Msa1204230:intron 25.0%
! AGTAATCATCCATTAGAATA+AGG - chr8_1:59743419-59743438 None:intergenic 25.0%
! AATGGATGATTACTATTGTA+TGG + chr8_1:59743425-59743444 Msa1204230:intron 25.0%
!!! TACTATAATTTCTAGTAGGT+TGG + chr8_1:59743488-59743507 Msa1204230:intron 25.0%
!! ACTTTAAGAAGAAACAAGTT+TGG - chr8_1:59743563-59743582 None:intergenic 25.0%
!! TTAAAGTTTCTGCTGATTAT+TGG + chr8_1:59743576-59743595 Msa1204230:intron 25.0%
! AATTTACACTTATCTGCATA+GGG - chr8_1:59743027-59743046 None:intergenic 25.0%
!!! CTCATTTTGATTATTGGTTT+TGG + chr8_1:59743179-59743198 Msa1204230:intron 25.0%
! AGTAATCATCCATTAGAATA+AGG - chr8_1:59743419-59743438 None:intergenic 25.0%
! AATGGATGATTACTATTGTA+TGG + chr8_1:59743425-59743444 Msa1204230:intron 25.0%
!!! TACTATAATTTCTAGTAGGT+TGG + chr8_1:59743488-59743507 Msa1204230:intron 25.0%
!! ACTTTAAGAAGAAACAAGTT+TGG - chr8_1:59743563-59743582 None:intergenic 25.0%
!! TTAAAGTTTCTGCTGATTAT+TGG + chr8_1:59743576-59743595 Msa1204230:intron 25.0%
GAATTTACACTTATCTGCAT+AGG - chr8_1:59743028-59743047 None:intergenic 30.0%
!!! TTTGAATGTGTGTCATTTTG+TGG + chr8_1:59743327-59743346 Msa1204230:intron 30.0%
TGATTCCATCCTTATTCTAA+TGG + chr8_1:59743407-59743426 Msa1204230:intron 30.0%
ATCATCCATTAGAATAAGGA+TGG - chr8_1:59743415-59743434 None:intergenic 30.0%
GATGAACATAATACAATAGC+TGG - chr8_1:59743536-59743555 None:intergenic 30.0%
CCTCAAAATTATTGATGAGT+CGG - chr8_1:59743617-59743636 None:intergenic 30.0%
AAGAGTCAGAACAATATACA+TGG - chr8_1:59743890-59743909 None:intergenic 30.0%
!!! AGGGTTTGTTGAATTTTCTA+TGG - chr8_1:59743984-59744003 None:intergenic 30.0%
CTAAGAAGAAACGTATACTT+GGG + chr8_1:59744050-59744069 Msa1204230:CDS 30.0%
GAATTTACACTTATCTGCAT+AGG - chr8_1:59743028-59743047 None:intergenic 30.0%
!!! TTTGAATGTGTGTCATTTTG+TGG + chr8_1:59743327-59743346 Msa1204230:intron 30.0%
TGATTCCATCCTTATTCTAA+TGG + chr8_1:59743407-59743426 Msa1204230:intron 30.0%
ATCATCCATTAGAATAAGGA+TGG - chr8_1:59743415-59743434 None:intergenic 30.0%
GATGAACATAATACAATAGC+TGG - chr8_1:59743536-59743555 None:intergenic 30.0%
CCTCAAAATTATTGATGAGT+CGG - chr8_1:59743617-59743636 None:intergenic 30.0%
AAGAGTCAGAACAATATACA+TGG - chr8_1:59743890-59743909 None:intergenic 30.0%
!!! AGGGTTTGTTGAATTTTCTA+TGG - chr8_1:59743984-59744003 None:intergenic 30.0%
CTAAGAAGAAACGTATACTT+GGG + chr8_1:59744050-59744069 Msa1204230:CDS 30.0%
! TGTCCAAGAAGTATAGCAAT+GGG + chr8_1:59742992-59743011 Msa1204230:CDS 35.0%
ATGAAGATCCTGCACAATTT+AGG + chr8_1:59743052-59743071 Msa1204230:CDS 35.0%
TGAAGATCCTGCACAATTTA+GGG + chr8_1:59743053-59743072 Msa1204230:CDS 35.0%
GATACAGGATATTGTTCCTT+TGG + chr8_1:59743114-59743133 Msa1204230:CDS 35.0%
AAAGTGACAGTTCTTTCCAA+AGG - chr8_1:59743133-59743152 None:intergenic 35.0%
!! TTGACAAAGCAAGTTCTGAA+TGG + chr8_1:59743380-59743399 Msa1204230:intron 35.0%
! CCGACTCATCAATAATTTTG+AGG + chr8_1:59743614-59743633 Msa1204230:intron 35.0%
TGTCTTCATCATCACAAATG+CGG - chr8_1:59743718-59743737 None:intergenic 35.0%
ATCATACACTTGCACACAAA+TGG - chr8_1:59743803-59743822 None:intergenic 35.0%
TGTTCTGACTCTTGAAGTAT+TGG + chr8_1:59743897-59743916 Msa1204230:intron 35.0%
AAATCAGAGTTACAAGTTCC+TGG + chr8_1:59743929-59743948 Msa1204230:intron 35.0%
CCAAGTATACGTTTCTTCTT+AGG - chr8_1:59744052-59744071 None:intergenic 35.0%
CCTAAGAAGAAACGTATACT+TGG + chr8_1:59744049-59744068 Msa1204230:CDS 35.0%
! TGTCCAAGAAGTATAGCAAT+GGG + chr8_1:59742992-59743011 Msa1204230:CDS 35.0%
ATGAAGATCCTGCACAATTT+AGG + chr8_1:59743052-59743071 Msa1204230:CDS 35.0%
TGAAGATCCTGCACAATTTA+GGG + chr8_1:59743053-59743072 Msa1204230:CDS 35.0%
GATACAGGATATTGTTCCTT+TGG + chr8_1:59743114-59743133 Msa1204230:CDS 35.0%
AAAGTGACAGTTCTTTCCAA+AGG - chr8_1:59743133-59743152 None:intergenic 35.0%
!! TTGACAAAGCAAGTTCTGAA+TGG + chr8_1:59743380-59743399 Msa1204230:intron 35.0%
! CCGACTCATCAATAATTTTG+AGG + chr8_1:59743614-59743633 Msa1204230:intron 35.0%
TGTCTTCATCATCACAAATG+CGG - chr8_1:59743718-59743737 None:intergenic 35.0%
ATCATACACTTGCACACAAA+TGG - chr8_1:59743803-59743822 None:intergenic 35.0%
TGTTCTGACTCTTGAAGTAT+TGG + chr8_1:59743897-59743916 Msa1204230:intron 35.0%
AAATCAGAGTTACAAGTTCC+TGG + chr8_1:59743929-59743948 Msa1204230:intron 35.0%
CCAAGTATACGTTTCTTCTT+AGG - chr8_1:59744052-59744071 None:intergenic 35.0%
CCTAAGAAGAAACGTATACT+TGG + chr8_1:59744049-59744068 Msa1204230:CDS 35.0%
! CTGTCCAAGAAGTATAGCAA+TGG + chr8_1:59742991-59743010 Msa1204230:CDS 40.0%
! GTCCAAGAAGTATAGCAATG+GGG + chr8_1:59742993-59743012 Msa1204230:CDS 40.0%
ATTGTGCAGGATCTTCATGA+AGG - chr8_1:59743050-59743069 None:intergenic 40.0%
GAAGATCCTGCACAATTTAG+GGG + chr8_1:59743054-59743073 Msa1204230:CDS 40.0%
!! CACAATTTAGGGGCACTTAT+CGG + chr8_1:59743064-59743083 Msa1204230:CDS 40.0%
!! AAGAACTGTCACTTTGCTCA+TGG + chr8_1:59743138-59743157 Msa1204230:CDS 40.0%
CTTATTTGACCGCGGTATTA+AGG - chr8_1:59743685-59743704 None:intergenic 40.0%
CTTAATACCGCGGTCAAATA+AGG + chr8_1:59743683-59743702 Msa1204230:intron 40.0%
! GCATTAACCTTATTTGACCG+CGG - chr8_1:59743693-59743712 None:intergenic 40.0%
AATTGCAGCTTCAGCTTCAA+TGG - chr8_1:59743960-59743979 None:intergenic 40.0%
!! TTTCCACAAGTTGACTTGCA+GGG - chr8_1:59744015-59744034 None:intergenic 40.0%
!!! TTTTCCACAAGTTGACTTGC+AGG - chr8_1:59744016-59744035 None:intergenic 40.0%
TGCAAGTCAACTTGTGGAAA+AGG + chr8_1:59744015-59744034 Msa1204230:CDS 40.0%
! CTGTCCAAGAAGTATAGCAA+TGG + chr8_1:59742991-59743010 Msa1204230:CDS 40.0%
! GTCCAAGAAGTATAGCAATG+GGG + chr8_1:59742993-59743012 Msa1204230:CDS 40.0%
ATTGTGCAGGATCTTCATGA+AGG - chr8_1:59743050-59743069 None:intergenic 40.0%
GAAGATCCTGCACAATTTAG+GGG + chr8_1:59743054-59743073 Msa1204230:CDS 40.0%
!! CACAATTTAGGGGCACTTAT+CGG + chr8_1:59743064-59743083 Msa1204230:CDS 40.0%
!! AAGAACTGTCACTTTGCTCA+TGG + chr8_1:59743138-59743157 Msa1204230:CDS 40.0%
CTTATTTGACCGCGGTATTA+AGG - chr8_1:59743685-59743704 None:intergenic 40.0%
CTTAATACCGCGGTCAAATA+AGG + chr8_1:59743683-59743702 Msa1204230:intron 40.0%
! GCATTAACCTTATTTGACCG+CGG - chr8_1:59743693-59743712 None:intergenic 40.0%
AATTGCAGCTTCAGCTTCAA+TGG - chr8_1:59743960-59743979 None:intergenic 40.0%
!! TTTCCACAAGTTGACTTGCA+GGG - chr8_1:59744015-59744034 None:intergenic 40.0%
!!! TTTTCCACAAGTTGACTTGC+AGG - chr8_1:59744016-59744035 None:intergenic 40.0%
TGCAAGTCAACTTGTGGAAA+AGG + chr8_1:59744015-59744034 Msa1204230:CDS 40.0%
CTCCCCATTGCTATACTTCT+TGG - chr8_1:59742998-59743017 None:intergenic 45.0%
! CAAGAAGTATAGCAATGGGG+AGG + chr8_1:59742996-59743015 Msa1204230:CDS 45.0%
TAAGTGCCCCTAAATTGTGC+AGG - chr8_1:59743063-59743082 None:intergenic 45.0%
AGACTAAGCTGTGTCTCAAG+TGG + chr8_1:59743088-59743107 Msa1204230:CDS 45.0%
TAAGCTGTGTCTCAAGTGGA+AGG + chr8_1:59743092-59743111 Msa1204230:CDS 45.0%
TGTCTCAAGTGGAAGGATAC+AGG + chr8_1:59743099-59743118 Msa1204230:CDS 45.0%
CACTTTGCTCATGGTGAAGA+AGG + chr8_1:59743147-59743166 Msa1204230:CDS 45.0%
TCAGAGTTACAAGTTCCTGG+TGG + chr8_1:59743932-59743951 Msa1204230:intron 45.0%
!! TTCCACAAGTTGACTTGCAG+GGG - chr8_1:59744014-59744033 None:intergenic 45.0%
CTCCCCATTGCTATACTTCT+TGG - chr8_1:59742998-59743017 None:intergenic 45.0%
! CAAGAAGTATAGCAATGGGG+AGG + chr8_1:59742996-59743015 Msa1204230:CDS 45.0%
TAAGTGCCCCTAAATTGTGC+AGG - chr8_1:59743063-59743082 None:intergenic 45.0%
AGACTAAGCTGTGTCTCAAG+TGG + chr8_1:59743088-59743107 Msa1204230:CDS 45.0%
TAAGCTGTGTCTCAAGTGGA+AGG + chr8_1:59743092-59743111 Msa1204230:CDS 45.0%
TGTCTCAAGTGGAAGGATAC+AGG + chr8_1:59743099-59743118 Msa1204230:CDS 45.0%
CACTTTGCTCATGGTGAAGA+AGG + chr8_1:59743147-59743166 Msa1204230:CDS 45.0%
TCAGAGTTACAAGTTCCTGG+TGG + chr8_1:59743932-59743951 Msa1204230:intron 45.0%
!! TTCCACAAGTTGACTTGCAG+GGG - chr8_1:59744014-59744033 None:intergenic 45.0%
CGGTATTAAGGATCGTGGCA+TGG - chr8_1:59743673-59743692 None:intergenic 50.0%
TGCCACGATCCTTAATACCG+CGG + chr8_1:59743673-59743692 Msa1204230:intron 50.0%
CGGTATTAAGGATCGTGGCA+TGG - chr8_1:59743673-59743692 None:intergenic 50.0%
TGCCACGATCCTTAATACCG+CGG + chr8_1:59743673-59743692 Msa1204230:intron 50.0%
GACCGCGGTATTAAGGATCG+TGG - chr8_1:59743678-59743697 None:intergenic 55.0%
! AAGGTCTAGGACCACGACTG+TGG - chr8_1:59743762-59743781 None:intergenic 55.0%
TCAGCTTCAATGGCTCCACC+AGG - chr8_1:59743950-59743969 None:intergenic 55.0%
!! AAGTTGACTTGCAGGGGAGG+TGG - chr8_1:59744008-59744027 None:intergenic 55.0%
!! CACAAGTTGACTTGCAGGGG+AGG - chr8_1:59744011-59744030 None:intergenic 55.0%
CTCCCCTGCAAGTCAACTTG+TGG + chr8_1:59744009-59744028 Msa1204230:CDS 55.0%
GACCGCGGTATTAAGGATCG+TGG - chr8_1:59743678-59743697 None:intergenic 55.0%
! AAGGTCTAGGACCACGACTG+TGG - chr8_1:59743762-59743781 None:intergenic 55.0%
TCAGCTTCAATGGCTCCACC+AGG - chr8_1:59743950-59743969 None:intergenic 55.0%
!! AAGTTGACTTGCAGGGGAGG+TGG - chr8_1:59744008-59744027 None:intergenic 55.0%
!! CACAAGTTGACTTGCAGGGG+AGG - chr8_1:59744011-59744030 None:intergenic 55.0%
CTCCCCTGCAAGTCAACTTG+TGG + chr8_1:59744009-59744028 Msa1204230:CDS 55.0%
GTACGTCTCAGCCACAGTCG+TGG + chr8_1:59743748-59743767 Msa1204230:intron 60.0%
GACTTGCAGGGGAGGTGGTA+GGG - chr8_1:59744003-59744022 None:intergenic 60.0%
! TGACTTGCAGGGGAGGTGGT+AGG - chr8_1:59744004-59744023 None:intergenic 60.0%
GTACGTCTCAGCCACAGTCG+TGG + chr8_1:59743748-59743767 Msa1204230:intron 60.0%
GACTTGCAGGGGAGGTGGTA+GGG - chr8_1:59744003-59744022 None:intergenic 60.0%
! TGACTTGCAGGGGAGGTGGT+AGG - chr8_1:59744004-59744023 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 59742985 59744102 59742985 ID=Msa1204230;Name=Msa1204230
chr8_1 mRNA 59742985 59744102 59742985 ID=Msa1204230-mRNA-1;Parent=Msa1204230;Name=Msa1204230-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|0|2|0|117
chr8_1 exon 59742985 59743168 59742985 ID=Msa1204230-mRNA-1:exon:19301;Parent=Msa1204230-mRNA-1
chr8_1 exon 59743936 59744102 59743936 ID=Msa1204230-mRNA-1:exon:19302;Parent=Msa1204230-mRNA-1
chr8_1 CDS 59742985 59743168 59742985 ID=Msa1204230-mRNA-1:cds;Parent=Msa1204230-mRNA-1
chr8_1 CDS 59743936 59744102 59743936 ID=Msa1204230-mRNA-1:cds;Parent=Msa1204230-mRNA-1
Gene Sequence

>Msa1204230

ATGAAACTGTCCAAGAAGTATAGCAATGGGGAGGAATGTCCCTATGCAGATAAGTGTAAATTCCTTCATGAAGATCCTGCACAATTTAGGGGCACTTATCGGAAGACTAAGCTGTGTCTCAAGTGGAAGGATACAGGATATTGTTCCTTTGGAAAGAACTGTCACTTTGCTCATGGTGAAGAAGAGTTACAAGTTCCTGGTGGAGCCATTGAAGCTGAAGCTGCAATTACCATAGAAAATTCAACAAACCCTACCACCTCCCCTGCAAGTCAACTTGTGGAAAAGGAAGAAGAGCAGCCTAAGAAGAAACGTATACTTGGGTTGAAATTCAGTCAGATCAATCGTATTTAC

Protein sequence

>Msa1204230

MKLSKKYSNGEECPYADKCKFLHEDPAQFRGTYRKTKLCLKWKDTGYCSFGKNCHFAHGEEELQVPGGAIEAEAAITIENSTNPTTSPASQLVEKEEEQPKKKRILGLKFSQINRIY