AlfalfaGEDB | Msa1205310

M. sativa cultivar zhongmu-4 / Msa1205310

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1205310	A0A2K3MEL2	69.231	117	30	2	55	166	3	118	1.26e-47	164

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TFs/TRs:

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Gene ID	Type	Classification
Msa1205310	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1205310	MtrunA17_Chr1g0203051	34.320	169	100	4	3	166	6	168	2.17e-21	90.1
Msa1205310	MtrunA17_Chr1g0171741	32.984	191	98	5	4	166	33	221	6.65e-21	86.3
Msa1205310	MtrunA17_Chr8g0389811	38.938	113	63	2	59	166	62	173	4.38e-20	82.8
Msa1205310	MtrunA17_Chr6g0473421	45.882	85	37	2	68	152	55	130	6.64e-19	78.6
Msa1205310	MtrunA17_Chr2g0331371	46.809	94	41	2	68	161	55	139	2.17e-18	78.6
Msa1205310	MtrunA17_Chr1g0172931	39.316	117	61	4	49	159	46	158	7.13e-16	70.9
Msa1205310	MtrunA17_Chr2g0287651	41.772	79	45	1	81	159	80	157	1.81e-14	70.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 41 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAACAATAGAACATGT+AGG	0.609954	8_1:-61484333	None:intergenic
AACACGATAGAAGAAAGTGA+TGG	0.695154	8_1:+61484068	Msa1205310:CDS
AACTGACGAATCTGAAGAGG+AGG	0.591955	8_1:+61484169	Msa1205310:CDS
AAGGGTTTAGGGAGAATAGG+GGG	0.661605	8_1:+61484450	Msa1205310:CDS
ACGTAAGGGTCAAATGATTA+GGG	0.438100	8_1:+61484379	Msa1205310:CDS
ACGTTTGTATGTTCATGTGA+AGG	0.528222	8_1:+61484431	Msa1205310:CDS
AGCAATTGTGTTCAACGATA+TGG	0.256143	8_1:+61484232	Msa1205310:CDS
ATGATTAGGGATCGTCAAGG+AGG	0.634745	8_1:+61484392	Msa1205310:CDS
ATGTTCATGTGAAGGGTTTA+GGG	0.296316	8_1:+61484439	Msa1205310:CDS
ATTCGTCAGTTTCGGCAGGC+AGG	0.483967	8_1:-61484157	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTAAAAGAATAGGCAAAGTT+AGG	+	chr8_1:61483257-61483276	Msa1205310:intron	30.0%
AGGAAGAATAACATGCAATA+TGG	-	chr8_1:61483670-61483689	None:intergenic	30.0%
GAGTAGTTGGCAAATTAAAA+TGG	-	chr8_1:61483739-61483758	None:intergenic	30.0%
GATGGTAGTAATATATCAAG+TGG	+	chr8_1:61484086-61484105	Msa1205310:CDS	30.0%
AAACCAACAATAGAACATGT+AGG	-	chr8_1:61484336-61484355	None:intergenic	30.0%
CTAAAAGAATAGGCAAAGTT+AGG	+	chr8_1:61483257-61483276	Msa1205310:intron	30.0%
AGGAAGAATAACATGCAATA+TGG	-	chr8_1:61483670-61483689	None:intergenic	30.0%
GAGTAGTTGGCAAATTAAAA+TGG	-	chr8_1:61483739-61483758	None:intergenic	30.0%
GATGGTAGTAATATATCAAG+TGG	+	chr8_1:61484086-61484105	Msa1205310:CDS	30.0%
AAACCAACAATAGAACATGT+AGG	-	chr8_1:61484336-61484355	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	61483184	61484568	61483184	ID=Msa1205310;Name=Msa1205310
chr8_1	mRNA	61483184	61484568	61483184	ID=Msa1205310-mRNA-1;Parent=Msa1205310;Name=Msa1205310-mRNA-1;_AED=0.44;_eAED=0.45;_QI=0\|0\|0\|0.75\|0.33\|0.5\|4\|0\|166
chr8_1	exon	61483184	61483189	61483184	ID=Msa1205310-mRNA-1:exon:19965;Parent=Msa1205310-mRNA-1
chr8_1	exon	61483945	61483950	61483945	ID=Msa1205310-mRNA-1:exon:19966;Parent=Msa1205310-mRNA-1
chr8_1	exon	61484068	61484306	61484068	ID=Msa1205310-mRNA-1:exon:19967;Parent=Msa1205310-mRNA-1
chr8_1	exon	61484322	61484568	61484322	ID=Msa1205310-mRNA-1:exon:19968;Parent=Msa1205310-mRNA-1
chr8_1	CDS	61483184	61483189	61483184	ID=Msa1205310-mRNA-1:cds;Parent=Msa1205310-mRNA-1
chr8_1	CDS	61483945	61483950	61483945	ID=Msa1205310-mRNA-1:cds;Parent=Msa1205310-mRNA-1
chr8_1	CDS	61484068	61484306	61484068	ID=Msa1205310-mRNA-1:cds;Parent=Msa1205310-mRNA-1
chr8_1	CDS	61484322	61484568	61484322	ID=Msa1205310-mRNA-1:cds;Parent=Msa1205310-mRNA-1

Gene Sequence

>Msa1205310

ATGTTAGTCTGGAACACGATAGAAGAAAGTGATGGTAGTAATATATCAAGTGGTGGATCTAGTAATGCAATCGTCTCTGGTAGTTCAAACTCACAAGTATCCCTGCCTGCCGAAACTGACGAATCTGAAGAGGAGGCAAATTCTTTTGACGAAGGAGAAGATGGTTCAGAAGGCGAAGCAATTGTGTTCAACGATATGGGTGATATAATGAAGTTTAATATGGAAAACTTTTGTGTGGATGATGTTGGAAGTTTGGATGTTGCCTACATGTTCTATTGTTGGTTTGCTAGAATGTCTGGGTTCTCTGTACGTAAGGGTCAAATGATTAGGGATCGTCAAGGAGGGGTAATTTTGCAGCAAACGTTTGTATGTTCATGTGAAGGGTTTAGGGAGAATAGGGGGCTAACCAGGGAGAACCGAAAGCGTGAGCCACGTAATGAAACAAGATGTGGATGTAAGGCAAAATTTAGAGTGCACGTAGACATTGTTTCGAAGCGT

Protein sequence

>Msa1205310

MLVWNTIEESDGSNISSGGSSNAIVSGSSNSQVSLPAETDESEEEANSFDEGEDGSEGEAIVFNDMGDIMKFNMENFCVDDVGSLDVAYMFYCWFARMSGFSVRKGQMIRDRQGGVILQQTFVCSCEGFRENRGLTRENRKREPRNETRCGCKAKFRVHVDIVSKR