AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1207810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1207810 MtrunA17_Chr4g0056541 95.038 262 7 2 1 262 109 364 0.0 516
Msa1207810 MtrunA17_Chr4g0018391 54.709 223 77 9 1 211 109 319 3.22e-66 209
Msa1207810 MtrunA17_Chr5g0398971 49.818 275 90 15 1 262 110 349 4.96e-64 204
Msa1207810 MtrunA17_Chr5g0405211 42.308 208 88 8 1 199 110 294 2.75e-33 124
Msa1207810 MtrunA17_Chr8g0372121 38.571 210 85 4 1 208 108 275 4.78e-33 122
Msa1207810 MtrunA17_Chr1g0200561 79.167 48 10 0 1 48 121 168 2.70e-21 91.7
Msa1207810 MtrunA17_Chr2g0309331 34.146 205 100 5 1 194 113 293 1.44e-20 89.4
Msa1207810 MtrunA17_Chr8g0346001 33.628 226 115 8 1 196 117 337 2.38e-19 86.7
Msa1207810 MtrunA17_Chr4g0017921 77.551 49 10 1 1 49 110 157 2.55e-19 85.9
Msa1207810 MtrunA17_Chr6g0484441 52.941 68 29 1 1 65 104 171 4.93e-17 79.3
Msa1207810 MtrunA17_Chr3g0139331 58.333 48 20 0 1 48 112 159 3.73e-15 74.3
Msa1207810 MtrunA17_Chr3g0091921 58.929 56 17 2 1 51 115 169 1.34e-14 72.0
Msa1207810 MtrunA17_Chr7g0214071 48.276 58 30 0 1 58 113 170 1.84e-14 71.2
Msa1207810 MtrunA17_Chr1g0183741 62.745 51 17 1 1 51 111 159 4.01e-14 70.5
Msa1207810 MtrunA17_Chr7g0261411 65.217 46 14 1 1 46 111 154 1.03e-13 69.3
Msa1207810 MtrunA17_Chr4g0060931 60.377 53 15 2 1 48 116 167 1.53e-13 69.7
Msa1207810 MtrunA17_Chr1g0147421 56.250 48 21 0 1 48 129 176 1.76e-13 69.3
Msa1207810 MtrunA17_Chr4g0042561 47.761 67 27 2 1 59 108 174 2.54e-13 68.2
Msa1207810 MtrunA17_Chr2g0312321 56.863 51 19 1 1 48 108 158 3.09e-13 68.2
Msa1207810 MtrunA17_Chr7g0217721 48.052 77 16 3 1 57 118 190 7.92e-13 67.4
Msa1207810 MtrunA17_Chr3g0113001 52.632 57 16 2 1 49 64 117 1.20e-12 66.6
Msa1207810 MtrunA17_Chr2g0309891 46.875 64 31 1 1 61 107 170 1.32e-12 66.6
Msa1207810 MtrunA17_Chr3g0113031 52.632 57 17 2 1 49 39 93 2.03e-12 65.5
Msa1207810 MtrunA17_Chr5g0417791 50.000 58 23 1 1 52 102 159 2.31e-12 65.5
Msa1207810 MtrunA17_Chr6g0453451 55.769 52 19 2 1 48 119 170 2.85e-12 65.5
Msa1207810 MtrunA17_Chr8g0364791 42.308 78 37 4 1 72 107 182 3.32e-12 65.9
Msa1207810 MtrunA17_Chr5g0417001 43.077 65 27 1 1 55 115 179 4.02e-12 65.5
Msa1207810 MtrunA17_Chr2g0318081 52.000 50 22 1 1 48 115 164 6.60e-12 64.7
Msa1207810 MtrunA17_Chr8g0361731 42.424 66 27 1 1 55 115 180 6.86e-12 64.7
Msa1207810 MtrunA17_Chr7g0253011 54.237 59 22 2 1 55 120 177 7.44e-12 64.7
Msa1207810 MtrunA17_Chr2g0316971 53.846 52 20 1 1 48 124 175 9.09e-12 64.3
Msa1207810 MtrunA17_Chr3g0109821 42.623 61 32 1 1 58 107 167 1.28e-11 64.3
Msa1207810 MtrunA17_Chr6g0481091 50.000 50 23 1 1 48 107 156 1.78e-11 62.4
Msa1207810 MtrunA17_Chr6g0461661 42.857 63 32 1 1 59 119 181 1.82e-11 63.5
Msa1207810 MtrunA17_Chr8g0359921 39.706 68 37 1 1 64 113 180 2.14e-11 63.2
Msa1207810 MtrunA17_Chr8g0384201 36.957 92 51 4 1 87 107 196 2.86e-11 63.2
Msa1207810 MtrunA17_Chr7g0252971 48.148 54 22 1 1 48 116 169 3.21e-11 62.8
Msa1207810 MtrunA17_Chr6g0453131 41.176 68 27 2 1 55 115 182 3.77e-11 62.8
Msa1207810 MtrunA17_Chr3g0130101 52.941 51 20 2 1 48 108 157 4.64e-11 62.4
Msa1207810 MtrunA17_Chr3g0131911 41.935 62 28 1 1 54 115 176 5.38e-11 61.2
Msa1207810 MtrunA17_Chr5g0433781 42.254 71 33 3 1 63 107 177 8.18e-11 62.0
Msa1207810 MtrunA17_Chr7g0263931 50.000 54 21 1 1 48 112 165 8.34e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1207810 AT1G12260.1 43.464 306 110 10 1 262 109 395 1.14e-63 204
Msa1207810 AT1G12260.2 43.071 267 114 10 1 262 109 342 3.62e-55 181
Msa1207810 AT1G62700.1 41.472 299 123 12 1 260 109 394 1.17e-52 176
Msa1207810 AT1G62700.2 41.472 299 123 12 1 260 109 394 1.17e-52 176
Msa1207810 AT2G18060.2 37.826 230 111 7 1 216 23 234 1.61e-36 130
Msa1207810 AT4G36160.2 37.603 242 108 7 1 217 116 339 1.46e-35 130
Msa1207810 AT4G36160.1 37.603 242 108 7 1 217 112 335 1.50e-35 130
Msa1207810 AT4G36160.3 37.603 242 108 7 1 217 112 335 1.50e-35 130
Msa1207810 AT2G18060.3 37.826 230 111 7 1 216 111 322 2.01e-35 130
Msa1207810 AT2G18060.1 37.826 230 111 7 1 216 111 322 2.01e-35 130
Msa1207810 AT5G66300.1 38.278 209 80 6 1 196 114 286 8.88e-32 119
Msa1207810 AT5G62380.2 35.537 242 109 8 1 220 109 325 7.02e-31 117
Msa1207810 AT5G62380.1 35.537 242 109 8 1 220 109 325 7.02e-31 117
Msa1207810 AT2G46770.1 34.934 229 93 9 1 213 118 306 5.30e-23 96.7
Msa1207810 AT1G32770.1 42.763 152 48 9 1 126 118 256 5.53e-20 88.2
Msa1207810 AT1G71930.2 75.000 48 12 0 1 48 111 158 5.94e-20 87.8
Msa1207810 AT1G71930.1 75.000 48 12 0 1 48 111 158 5.94e-20 87.8
Msa1207810 AT3G61910.1 32.227 211 79 7 1 196 113 274 1.51e-18 84.0
Msa1207810 AT1G79580.3 73.469 49 12 1 1 49 120 167 1.53e-18 84.3
Msa1207810 AT1G79580.5 73.469 49 12 1 1 49 120 167 1.53e-18 84.3
Msa1207810 AT1G79580.4 73.469 49 12 1 1 49 120 167 1.53e-18 84.3
Msa1207810 AT1G79580.2 73.469 49 12 1 1 49 120 167 1.53e-18 84.3
Msa1207810 AT1G79580.1 73.469 49 12 1 1 49 120 167 1.53e-18 84.3
Msa1207810 AT1G33280.1 73.469 49 12 1 1 49 110 157 1.86e-18 83.6
Msa1207810 AT4G10350.2 70.588 51 13 1 1 49 64 114 1.15e-17 80.9
Msa1207810 AT4G10350.1 70.588 51 13 1 1 49 111 161 1.90e-17 80.9
Msa1207810 AT3G18400.1 70.000 50 12 2 1 48 104 152 3.53e-16 77.0
Msa1207810 AT3G10490.4 39.785 93 48 3 1 87 28 118 5.25e-15 74.3
Msa1207810 AT3G10490.3 39.785 93 48 3 1 87 28 118 5.25e-15 74.3
Msa1207810 AT3G10490.1 39.785 93 48 3 1 87 128 218 6.44e-15 72.4
Msa1207810 AT3G10490.2 39.785 93 48 3 1 87 128 218 1.00e-14 73.9
Msa1207810 AT2G24430.2 58.491 53 21 1 1 52 117 169 2.07e-14 72.0
Msa1207810 AT2G24430.1 58.491 53 21 1 1 52 117 169 2.07e-14 72.0
Msa1207810 AT1G56010.1 58.491 53 17 1 1 48 52 104 3.79e-14 70.9
Msa1207810 AT3G12977.2 58.824 51 18 1 1 48 64 114 6.29e-14 69.7
Msa1207810 AT3G10480.1 38.710 93 49 3 1 87 128 218 6.32e-14 71.2
Msa1207810 AT1G56010.2 58.491 53 17 1 1 48 119 171 6.74e-14 70.9
Msa1207810 AT1G69490.1 54.545 55 19 1 1 49 108 162 8.23e-14 70.1
Msa1207810 AT3G10480.3 38.710 93 49 3 1 87 161 251 9.81e-14 70.9
Msa1207810 AT5G61430.2 55.769 52 19 1 1 48 52 103 1.50e-13 69.3
Msa1207810 AT3G12977.1 58.824 51 18 1 1 48 117 167 1.66e-13 69.3
Msa1207810 AT3G04060.1 37.391 115 54 6 1 100 120 231 1.68e-13 69.7
Msa1207810 AT4G28530.1 64.583 48 15 1 1 48 127 172 2.06e-13 69.7
Msa1207810 AT5G61430.1 55.769 52 19 1 1 48 115 166 2.88e-13 68.9
Msa1207810 AT5G18270.1 54.839 62 22 2 1 56 121 182 3.34e-13 68.9
Msa1207810 AT5G18270.2 54.839 62 22 2 1 56 121 182 3.37e-13 68.9
Msa1207810 AT5G39610.1 59.259 54 16 2 1 49 119 171 3.74e-13 68.2
Msa1207810 AT3G10480.2 39.785 93 47 4 1 87 128 217 5.28e-13 68.6
Msa1207810 AT1G76420.1 61.224 49 18 1 1 48 123 171 6.48e-13 68.2
Msa1207810 AT5G04410.1 38.750 80 43 3 1 75 110 188 8.28e-13 68.2
Msa1207810 AT3G15500.1 50.877 57 25 2 1 55 113 168 1.36e-12 67.0
Msa1207810 AT5G07680.2 53.846 52 20 1 1 48 102 153 1.37e-12 67.0
Msa1207810 AT5G07680.1 53.846 52 20 1 1 48 116 167 1.47e-12 67.0
Msa1207810 AT5G53950.1 57.692 52 18 1 1 48 118 169 1.77e-12 67.0
Msa1207810 AT3G04070.2 34.694 98 41 3 1 78 114 208 1.84e-12 66.6
Msa1207810 AT1G52890.1 43.284 67 34 2 1 65 113 177 2.94e-12 65.9
Msa1207810 AT2G33480.3 52.941 51 22 2 1 49 54 104 3.64e-12 64.3
Msa1207810 AT2G33480.2 53.061 49 23 0 1 49 111 159 4.38e-12 65.1
Msa1207810 AT3G10500.1 36.905 84 46 3 1 78 110 192 8.17e-12 65.5
Msa1207810 AT2G33480.1 52.941 51 22 2 1 49 111 161 9.13e-12 63.9
Msa1207810 AT3G17730.1 54.000 50 21 1 1 48 107 156 9.92e-12 63.5
Msa1207810 AT4G27410.2 52.000 50 22 1 1 48 113 162 1.54e-11 63.5
Msa1207810 AT1G65910.1 52.941 51 18 2 2 48 108 156 1.81e-11 64.3
Msa1207810 AT4G27410.3 52.000 50 22 1 1 48 130 179 1.88e-11 63.5
Msa1207810 AT5G13180.1 50.000 50 24 1 1 49 112 161 2.06e-11 62.8
Msa1207810 AT3G15170.1 47.541 61 28 1 1 57 121 181 5.52e-11 62.0
Msa1207810 AT1G32510.1 47.368 57 27 1 2 55 115 171 6.20e-11 62.0
Msa1207810 AT5G17260.1 39.683 63 35 1 2 61 108 170 6.48e-11 62.4
Msa1207810 AT5G46590.1 58.000 50 18 1 2 48 109 158 9.45e-11 61.2

Find 118 sgRNAs with CRISPR-Local

Find 327 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATCTTCACTCACTTTA+TGG 0.109284 8_1:+65592430 Msa1207810:CDS
GCATATTAATATATAGTATT+TGG 0.147682 8_1:+65592766 Msa1207810:three_prime_UTR
AAGAGAAAAGATGATTTATT+AGG 0.185077 8_1:+65586385 Msa1207810:five_prime_UTR
GGTGAGAATTGGTTCGGGTT+GGG 0.250402 8_1:-65592177 Msa1207820:intron
CAAACAGAATTATGCAAAAT+AGG 0.259681 8_1:+65588267 Msa1207810:intron
CCTTTATCTATTGTTAAGTT+TGG 0.271628 8_1:-65586345 None:intergenic
TATATATGTAGGAACATTTC+CGG 0.273565 8_1:-65592956 Msa1207820:intron
CAATCAGATTTCTGTCCATT+TGG 0.277247 8_1:-65588473 None:intergenic
TAAGTTTGGAGCTAGATTTC+AGG 0.284365 8_1:-65586331 None:intergenic
GCATGAGCATGATGATTATA+AGG 0.293277 8_1:-65592337 Msa1207820:intron
GAGCAACAAAAGCAGGATTC+TGG 0.295790 8_1:+65588364 Msa1207810:five_prime_UTR
TATTGCATTAATTCTAGTTC+TGG 0.299193 8_1:-65592232 Msa1207820:intron
AATCAGATTTCTGTCCATTT+GGG 0.310097 8_1:-65588472 None:intergenic
CAAATCACTCTTCTTGATAA+TGG 0.311351 8_1:+65592631 Msa1207810:CDS
GTGAGAATTGGTTCGGGTTG+GGG 0.323739 8_1:-65592176 Msa1207820:intron
AGAGAAAAGATGATTTATTA+GGG 0.328786 8_1:+65586386 Msa1207810:five_prime_UTR
CAATATACTCAAAACAATTC+TGG 0.331709 8_1:+65588409 Msa1207810:five_prime_UTR
CGTCTTGAAACTAATCAAAA+TGG 0.345940 8_1:+65588512 Msa1207810:CDS
TATTAAAATTAAAATACATT+TGG 0.358715 8_1:-65592911 Msa1207820:intron
ATGAACAGCTTACAGCATTA+AGG 0.362569 8_1:-65592850 Msa1207820:intron
TGGGAGGGTGAGAATTGGTT+CGG 0.374156 8_1:-65592183 Msa1207820:intron
ATATATGAACCTCTGGAGTC+TGG 0.395962 8_1:+65592873 Msa1207810:three_prime_UTR
ATGATCAATCACCATGTAAC+TGG 0.402350 8_1:+65592116 Msa1207810:CDS
CCAAACTTAACAATAGATAA+AGG 0.415115 8_1:+65586345 Msa1207810:five_prime_UTR
CATGAGCATGATGATTATAA+GGG 0.417661 8_1:-65592336 Msa1207820:intron
CCAGAGTTCCATGTGGCAAC+TGG 0.420939 8_1:-65592701 Msa1207820:intron
GGCAATCGATGATCAGTTGC+CGG 0.421864 8_1:+65592937 Msa1207810:three_prime_UTR
ATAGTTGGGAGGGTGAGAAT+TGG 0.428152 8_1:-65592188 Msa1207820:intron
GGGAGGGTGAGAATTGGTTC+GGG 0.428982 8_1:-65592182 Msa1207820:intron
GAAAACTAGCATCATGGTAT+TGG 0.436544 8_1:-65592267 Msa1207820:intron
GGCTTTCAAGGTGTGGAAGT+TGG 0.437985 8_1:-65592291 Msa1207820:intron
CGGGTTGGGGATTCAAGATC+TGG 0.439654 8_1:-65592163 Msa1207820:intron
ATTTATTGTTATATATATGT+AGG 0.441656 8_1:-65592967 Msa1207820:intron
GAAAAGCTTATCTCATGAAA+TGG 0.442191 8_1:-65586309 None:intergenic
CAGTAATGGCAATGATGAAC+AGG 0.444844 8_1:+65592462 Msa1207810:CDS
CTATTCGTTCTAGTTCCTGT+TGG 0.449896 8_1:-65588342 None:intergenic
TACTCAAAACAATTCTGGTT+GGG 0.452636 8_1:+65588414 Msa1207810:five_prime_UTR
AAGTTAAATTGTTGTATCCT+CGG 0.452739 8_1:-65586574 None:intergenic
AAGAAGGATGGGTTGTATGC+AGG 0.452985 8_1:+65592050 Msa1207810:CDS
CTAGTTCTGGTTTGCATGAG+TGG 0.455930 8_1:-65592219 Msa1207820:intron
ATGAAATGGTGGCGCTAAAA+CGG 0.457017 8_1:-65586295 None:intergenic
TGAGTGGTATGAAGTATAGT+TGG 0.457479 8_1:-65592203 Msa1207820:intron
ATCCACACTTGAATTCTACT+TGG 0.461676 8_1:+65586795 Msa1207810:five_prime_UTR
TGCATCCACACTTAATATCA+AGG 0.462463 8_1:+65586824 Msa1207810:five_prime_UTR
TAATCAAAATGGAATTTCTC+AGG 0.470538 8_1:+65588523 Msa1207810:CDS
GAGTGGTATGAAGTATAGTT+GGG 0.471759 8_1:-65592202 Msa1207820:intron
TTCTCTCCAACGAGAGGGAG+AGG 0.474187 8_1:-65586526 None:intergenic
AGTTAAATTGTTGTATCCTC+GGG 0.477142 8_1:-65586573 None:intergenic
AACTAGCATCATGGTATTGG+TGG 0.481901 8_1:-65592264 Msa1207820:intron
AGAAGGATGGGTTGTATGCA+GGG 0.483470 8_1:+65592051 Msa1207810:CDS
AAAGATAAGAAGTATCCAAC+AGG 0.484211 8_1:+65588327 Msa1207810:five_prime_UTR
GGGTGAGAATTGGTTCGGGT+TGG 0.484907 8_1:-65592178 Msa1207820:intron
TACAAAGGACGTGCCCCAAA+TGG 0.485596 8_1:+65588458 Msa1207810:CDS
GCTTATTATGTACACCAACT+TGG 0.486124 8_1:-65592808 Msa1207820:intron
ACGAATAGAGCAACAAAAGC+AGG 0.489114 8_1:+65588357 Msa1207810:five_prime_UTR
CCAGTTGCCACATGGAACTC+TGG 0.492908 8_1:+65592701 Msa1207810:CDS
TCCCGAGAGGAGTACTGAAA+AGG 0.495443 8_1:-65586472 None:intergenic
ATTGAACCATGGGATCTTCA+AGG 0.495896 8_1:+65587999 Msa1207810:five_prime_UTR
ATGAAAGTGAAGGAAGGAAA+AGG 0.498702 8_1:-65586442 None:intergenic
TCAACAAGTTCTTCATCAGT+TGG 0.499544 8_1:-65587903 None:intergenic
AACTTGTTGATTACTACCTC+AGG 0.500136 8_1:+65587916 Msa1207810:five_prime_UTR
CAAATGGACAGAAATCTGAT+TGG 0.510953 8_1:+65588474 Msa1207810:CDS
GTTGGATGAAAACGAAAGCC+TGG 0.518219 8_1:-65587885 None:intergenic
ATACTCAAAACAATTCTGGT+TGG 0.524363 8_1:+65588413 Msa1207810:five_prime_UTR
TGTTCATATATATGAACCTC+TGG 0.524534 8_1:+65592866 Msa1207810:three_prime_UTR
TATTGCCTTGTCTCTTCCTG+TGG 0.528014 8_1:-65588391 None:intergenic
TCTCTATAAAATTGAACCAT+GGG 0.531197 8_1:+65587989 Msa1207810:five_prime_UTR
TGATTACTACCTCAGGAAGA+AGG 0.532222 8_1:+65587923 Msa1207810:five_prime_UTR
ATGATGAACAGGTTACTGAT+TGG 0.534253 8_1:+65592473 Msa1207810:CDS
AACAGACAGGAAATGAAAAG+TGG 0.540578 8_1:-65586598 None:intergenic
GAAGAGATGAAAGTGAAGGA+AGG 0.540814 8_1:-65586448 None:intergenic
TTACCATGCTTATATATCTG+AGG 0.547268 8_1:+65586742 Msa1207810:five_prime_UTR
CACATGAAGAATAGCTGCAT+TGG 0.548314 8_1:-65592602 Msa1207820:intron
CTTTGTTCTCTCCAACGAGA+GGG 0.551086 8_1:-65586531 None:intergenic
ATCTCTATAAAATTGAACCA+TGG 0.552588 8_1:+65587988 Msa1207810:five_prime_UTR
ACTAGCATCATGGTATTGGT+GGG 0.553019 8_1:-65592263 Msa1207820:intron
GAAAAGGTAAGTTTCAAGAG+TGG 0.554530 8_1:-65586426 None:intergenic
CTCTCCAACGAGAGGGAGAG+GGG 0.557731 8_1:-65586524 None:intergenic
TATGGTGGTAACAGCAGTAA+TGG 0.564737 8_1:+65592448 Msa1207810:CDS
AGGATTTAGTGCTACAAACA+AGG 0.570577 8_1:+65586762 Msa1207810:five_prime_UTR
TAACTTGTACTGCAGGAAGA+AGG 0.571751 8_1:+65592034 Msa1207810:intron
CAATGCAGCTATTCTTCATG+TGG 0.572344 8_1:+65592603 Msa1207810:CDS
TTGTACTGCAGGAAGAAGGA+TGG 0.572577 8_1:+65592038 Msa1207810:intron
CACAAGGGGACAAAACAGAC+AGG 0.572681 8_1:-65586611 None:intergenic
ATCAGATTTCTGTCCATTTG+GGG 0.575645 8_1:-65588471 None:intergenic
AATAACCTTGATATTAAGTG+TGG 0.576631 8_1:-65586829 None:intergenic
GAAGTGATGAACAAAATGAC+TGG 0.576909 8_1:+65588289 Msa1207810:five_prime_UTR
TCTTTGTTCTCTCCAACGAG+AGG 0.577297 8_1:-65586532 None:intergenic
GCAGGATTCTGGAAAGCCAC+AGG 0.582522 8_1:+65588375 Msa1207810:five_prime_UTR
AGGAGAAGAGATGAAAGTGA+AGG 0.582728 8_1:-65586452 None:intergenic
GTTGGAGAAAACTAGCATCA+TGG 0.589690 8_1:-65592273 Msa1207820:intron
TCTCTCCAACGAGAGGGAGA+GGG 0.591885 8_1:-65586525 None:intergenic
TTCTACCTCCAGTTGCCACA+TGG 0.595602 8_1:+65592693 Msa1207810:CDS
GGATTCAAGATCTGGCATGA+AGG 0.597988 8_1:-65592155 Msa1207820:intron
GGTATGAAGTATAGTTGGGA+GGG 0.605203 8_1:-65592198 Msa1207820:intron
TGTACTGCAGGAAGAAGGAT+GGG 0.607538 8_1:+65592039 Msa1207810:intron
TGATCATCATACCAGTTACA+TGG 0.608098 8_1:-65592127 Msa1207820:intron
TCGATACCTTGAAGATCCCA+TGG 0.613286 8_1:-65588005 None:intergenic
CGTTGGAGAGAACAAAGAAG+AGG 0.614942 8_1:+65586537 Msa1207810:five_prime_UTR
CTACTACTTCAAAACGATCG+TGG 0.616988 8_1:+65586691 Msa1207810:five_prime_UTR
TCTCCAACGAGAGGGAGAGG+GGG 0.622124 8_1:-65586523 None:intergenic
GAGTTCCATGTGGCAACTGG+AGG 0.627769 8_1:-65592698 Msa1207820:CDS
AATCTTCACTCACTTTATGG+TGG 0.628619 8_1:+65592433 Msa1207810:CDS
TGATGAGTATTGCTCCAAGT+TGG 0.635184 8_1:+65592794 Msa1207810:three_prime_UTR
AATCCTCAGATATATAAGCA+TGG 0.635473 8_1:-65586745 None:intergenic
AAGCTTATCTCATGAAATGG+TGG 0.636207 8_1:-65586306 None:intergenic
CTAGCATCATGGTATTGGTG+GGG 0.638906 8_1:-65592262 Msa1207820:intron
TAGACGATACCTTCTTCCTG+AGG 0.649052 8_1:-65587932 None:intergenic
AAGAAAGGCAGAAAGACACA+AGG 0.658318 8_1:-65586627 None:intergenic
GAAAGCCACAGGAAGAGACA+AGG 0.659463 8_1:+65588386 Msa1207810:five_prime_UTR
GAAAGGCAGAAAGACACAAG+GGG 0.665624 8_1:-65586625 None:intergenic
CTCCAAGTAGAATTCAAGTG+TGG 0.669661 8_1:-65586797 None:intergenic
AGAAAGGCAGAAAGACACAA+GGG 0.670147 8_1:-65586626 None:intergenic
TGGTATGAAGTATAGTTGGG+AGG 0.680399 8_1:-65592199 Msa1207820:intron
GAGAGAGATCAGATCCCGAG+AGG 0.701888 8_1:-65586485 None:intergenic
TTCACTTCCAGAGTTCCATG+TGG 0.701916 8_1:-65592708 Msa1207820:intron
TATATATATCCAGACTCCAG+AGG 0.705194 8_1:-65592882 Msa1207820:intron
GGATGAAAACGAAAGCCTGG+AGG 0.739180 8_1:-65587882 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATAATAATAATAA+GGG - chr8_1:65592541-65592560 Msa1207820:intron 0.0%
!! TAATAATAATAATAATAATA+AGG - chr8_1:65592543-65592562 Msa1207820:intron 0.0%
!! TTATTATTATTATAAAATTT+AGG + chr8_1:65588986-65589005 Msa1207810:intron 0.0%
!! TATTATTATTATAAAATTTA+GGG + chr8_1:65588987-65589006 Msa1207810:intron 0.0%
!!! AAATATTAAATATTTTTTTA+AGG - chr8_1:65590744-65590763 None:intergenic 0.0%
!!! TTTTATTTTTTTATTTTTTA+AGG - chr8_1:65591093-65591112 Msa1207820:intron 0.0%
!!! AATATAAATTAATTTTGTTG+AGG - chr8_1:65587033-65587052 None:intergenic 10.0%
!! CTATTAAAAAAAAAAAAAAC+TGG - chr8_1:65587218-65587237 None:intergenic 10.0%
!!! TTTTTTTTTTTTTAATAGCT+AGG + chr8_1:65587220-65587239 Msa1207810:intron 10.0%
!! AAAAAATATTAAATATGGAA+AGG - chr8_1:65587328-65587347 None:intergenic 10.0%
!! TAAATGAAAAAATAATAATG+TGG + chr8_1:65587707-65587726 Msa1207810:intron 10.0%
!! TTTATTAAATGATAGTTATA+AGG - chr8_1:65589500-65589519 None:intergenic 10.0%
!! TATTTAGTAAATATATTTCA+AGG - chr8_1:65589722-65589741 None:intergenic 10.0%
!!! TGTATTTTAATTTTAATACT+AGG + chr8_1:65592916-65592935 Msa1207810:three_prime_UTR 10.0%
!! ATTTATTGTTATATATATGT+AGG - chr8_1:65592970-65592989 Msa1207820:intron 10.0%
!! TTAAAAAAAAAAAAAACTGG+TGG - chr8_1:65587215-65587234 None:intergenic 15.0%
!!! ACTTTTTACAATGTAATTAT+GGG - chr8_1:65587476-65587495 None:intergenic 15.0%
!!! ATCATTAATTTTCAACAATA+AGG - chr8_1:65587677-65587696 None:intergenic 15.0%
!! AAAAAAATAATTAACAAAGG+AGG - chr8_1:65587805-65587824 None:intergenic 15.0%
!!! AAAAAAATTCTGAGATAATA+TGG - chr8_1:65588066-65588085 None:intergenic 15.0%
!! TGAAAACAAAAAATTATACT+AGG + chr8_1:65588088-65588107 Msa1207810:intron 15.0%
!!! TAATAAAATGTTACATCTTT+TGG + chr8_1:65588849-65588868 Msa1207810:intron 15.0%
!!! ATAAAATTTAGGGTTAAATA+AGG + chr8_1:65588997-65589016 Msa1207810:intron 15.0%
!! CAATATTATTTATAATGATG+AGG + chr8_1:65589401-65589420 Msa1207810:intron 15.0%
!!! TTACTTATTTTGATAAGAAA+TGG + chr8_1:65589635-65589654 Msa1207810:intron 15.0%
!! TCGTAAAAATAAAAAAATCA+TGG - chr8_1:65589683-65589702 None:intergenic 15.0%
!! AAAAATTACGTATTAATTAG+CGG - chr8_1:65589759-65589778 None:intergenic 15.0%
!! TAAAAAAAATCTTATGTGAT+CGG + chr8_1:65590234-65590253 Msa1207810:intron 15.0%
!! AGAAATGAAAAATATATTAG+AGG - chr8_1:65590407-65590426 None:intergenic 15.0%
!! AAAAAATAAAATCAATCCAA+TGG + chr8_1:65591102-65591121 Msa1207810:intron 15.0%
!! CTTTATGATGTAAAATATTA+AGG - chr8_1:65591168-65591187 Msa1207820:intron 15.0%
!!! CTTAATATTTTACATCATAA+AGG + chr8_1:65591166-65591185 Msa1207810:intron 15.0%
!! GATTGTATATTAAATAACAA+GGG + chr8_1:65591198-65591217 Msa1207810:intron 15.0%
!!! TAATAGATTGAGTAATTTTT+GGG - chr8_1:65592315-65592334 Msa1207820:intron 15.0%
!!! ATAATAGATTGAGTAATTTT+TGG - chr8_1:65592316-65592335 Msa1207820:intron 15.0%
!! GCATATTAATATATAGTATT+TGG + chr8_1:65592766-65592785 Msa1207810:three_prime_UTR 15.0%
!! AAGAGAAAAGATGATTTATT+AGG + chr8_1:65586385-65586404 Msa1207810:five_prime_UTR 20.0%
!! AGAGAAAAGATGATTTATTA+GGG + chr8_1:65586386-65586405 Msa1207810:five_prime_UTR 20.0%
!! CTTGATCTAGTATATTATAT+GGG + chr8_1:65586899-65586918 Msa1207810:intron 20.0%
!! TAATATAAATACCAGAATTG+TGG + chr8_1:65586981-65587000 Msa1207810:intron 20.0%
!!! TTATGATCAACTTATTTTGT+AGG + chr8_1:65587384-65587403 Msa1207810:intron 20.0%
!!! CTTTTTACAATGTAATTATG+GGG - chr8_1:65587475-65587494 None:intergenic 20.0%
!!! CACTTTTTACAATGTAATTA+TGG - chr8_1:65587477-65587496 None:intergenic 20.0%
!!! ATTGATTCATATTTCCTTAA+AGG - chr8_1:65587541-65587560 None:intergenic 20.0%
!! ATATGAATCAATGTTTCATA+AGG + chr8_1:65587549-65587568 Msa1207810:intron 20.0%
!! CTTATAACAAAAGAAAAAGA+AGG - chr8_1:65587775-65587794 None:intergenic 20.0%
!!! TTATTTTTTTTTATGAGTGC+AGG + chr8_1:65587815-65587834 Msa1207810:intron 20.0%
!!! ATAATATGGATTGATTGTTA+AGG - chr8_1:65588052-65588071 None:intergenic 20.0%
!!! TCTTCTTTTTGCTTTTTTTA+TGG + chr8_1:65588565-65588584 Msa1207810:intron 20.0%
!! AGACTAAATCTTAATCATTT+AGG + chr8_1:65588677-65588696 Msa1207810:intron 20.0%
!! AACACAAATATAAATCACTT+AGG - chr8_1:65588794-65588813 None:intergenic 20.0%
!! ATATGTTTGCAAAATTTCAA+AGG - chr8_1:65589132-65589151 None:intergenic 20.0%
!!! AATTTTGCAAACATATTTAG+TGG + chr8_1:65589136-65589155 Msa1207810:intron 20.0%
!!! TCTAGCAATTTTTTTTAACA+AGG + chr8_1:65589173-65589192 Msa1207810:intron 20.0%
!! AAATTTATTCAAAAACGTGA+AGG + chr8_1:65589299-65589318 Msa1207810:intron 20.0%
!! AATTTATTCAAAAACGTGAA+GGG + chr8_1:65589300-65589319 Msa1207810:intron 20.0%
!! ATTTCCATAAATTTGTATTC+TGG - chr8_1:65590032-65590051 None:intergenic 20.0%
!!! TTCATTGCATTAGTTTTATA+TGG + chr8_1:65590105-65590124 Msa1207810:intron 20.0%
!!! TTTTTCATTTCTTCCATATT+AGG + chr8_1:65590415-65590434 Msa1207810:intron 20.0%
!! AAAACAAAATCATCCTAATA+TGG - chr8_1:65590431-65590450 None:intergenic 20.0%
!!! TCATGTCTTCTTTTTTATTT+CGG + chr8_1:65590490-65590509 Msa1207810:intron 20.0%
!! TAAATGTAAAATGACGTAAA+AGG + chr8_1:65590670-65590689 Msa1207810:intron 20.0%
!!! TCTTTTTAATTTCTGCAAAT+TGG - chr8_1:65590789-65590808 None:intergenic 20.0%
!!! AAAATTTTGTAACATCATGA+GGG + chr8_1:65590808-65590827 Msa1207810:intron 20.0%
!! ACAAATATAGTTTAATTCTC+TGG - chr8_1:65590866-65590885 None:intergenic 20.0%
!! TACCAAAAGAAATATCAAAT+TGG + chr8_1:65590908-65590927 Msa1207810:intron 20.0%
!!! ATCCAATTTGATATTTCTTT+TGG - chr8_1:65590913-65590932 None:intergenic 20.0%
!!! TCCAATTTGATTATTTCTTT+TGG - chr8_1:65591029-65591048 None:intergenic 20.0%
!!! TTATTTTTTAAGGAAACCAT+TGG - chr8_1:65591083-65591102 Msa1207820:intron 20.0%
!! GATGTAAAATATTAAGGAAA+CGG - chr8_1:65591162-65591181 Msa1207820:intron 20.0%
!! GGATTGTATATTAAATAACA+AGG + chr8_1:65591197-65591216 Msa1207810:intron 20.0%
!! TTAAATAACAAGGGTAAAAA+TGG + chr8_1:65591207-65591226 Msa1207810:intron 20.0%
!! TAGACTATATTTAAGAGTTT+AGG - chr8_1:65591452-65591471 Msa1207820:intron 20.0%
!!! TCTTATCTATCTTTTTCTAT+TGG - chr8_1:65591496-65591515 Msa1207820:intron 20.0%
!! ATACGCAATAAAATTTATTG+TGG + chr8_1:65591578-65591597 Msa1207810:intron 20.0%
!! AAATATATGTTGAAAGATTG+TGG - chr8_1:65591619-65591638 Msa1207820:intron 20.0%
!!! ATATTTTGTTAAACTACACT+AGG + chr8_1:65591902-65591921 Msa1207810:intron 20.0%
! CCTTTATCTATTGTTAAGTT+TGG - chr8_1:65586348-65586367 None:intergenic 25.0%
! CCAAACTTAACAATAGATAA+AGG + chr8_1:65586345-65586364 Msa1207810:five_prime_UTR 25.0%
! AAGTTAAATTGTTGTATCCT+CGG - chr8_1:65586577-65586596 None:intergenic 25.0%
! AATAACCTTGATATTAAGTG+TGG - chr8_1:65586832-65586851 None:intergenic 25.0%
! GCTTGATCTAGTATATTATA+TGG + chr8_1:65586898-65586917 Msa1207810:intron 25.0%
! TCTAGTATATTATATGGGTT+TGG + chr8_1:65586904-65586923 Msa1207810:intron 25.0%
! AAAAAAAAAAACTGGTGGAA+AGG - chr8_1:65587210-65587229 None:intergenic 25.0%
! ATGTTTAAACGTGATTTACT+AGG + chr8_1:65587355-65587374 Msa1207810:intron 25.0%
!! TAAAGGAGTATAACTAAACT+AGG - chr8_1:65587524-65587543 None:intergenic 25.0%
! AGTTTAGTTATACTCCTTTA+AGG + chr8_1:65587524-65587543 Msa1207810:intron 25.0%
! TGTTTCATAAGGTAAAGTTA+TGG + chr8_1:65587560-65587579 Msa1207810:intron 25.0%
! GTTTCATAAGGTAAAGTTAT+GGG + chr8_1:65587561-65587580 Msa1207810:intron 25.0%
!!! TCTCTCTTCCTATTTTTATT+TGG + chr8_1:65587615-65587634 Msa1207810:intron 25.0%
! ATCTCTATAAAATTGAACCA+TGG + chr8_1:65587988-65588007 Msa1207810:five_prime_UTR 25.0%
! TCTCTATAAAATTGAACCAT+GGG + chr8_1:65587989-65588008 Msa1207810:five_prime_UTR 25.0%
!! AAAATTATACTAGGAGTACA+AGG + chr8_1:65588097-65588116 Msa1207810:intron 25.0%
! CAAACAGAATTATGCAAAAT+AGG + chr8_1:65588267-65588286 Msa1207810:intron 25.0%
! CAATATACTCAAAACAATTC+TGG + chr8_1:65588409-65588428 Msa1207810:five_prime_UTR 25.0%
!! AAGACTCTAGTATTTTACAA+AGG + chr8_1:65588443-65588462 Msa1207810:CDS 25.0%
!!! CATTTTGATTAGTTTCAAGA+CGG - chr8_1:65588514-65588533 None:intergenic 25.0%
! TAATCAAAATGGAATTTCTC+AGG + chr8_1:65588523-65588542 Msa1207810:CDS 25.0%
! ATTCTCATACAAATACAAGA+AGG + chr8_1:65588701-65588720 Msa1207810:intron 25.0%
! AAAAAATGGGTACTAAAGAT+GGG - chr8_1:65588883-65588902 None:intergenic 25.0%
!!! TTGTTTTTAAGTCAACTCAT+GGG - chr8_1:65589328-65589347 None:intergenic 25.0%
!!! AAGTTGTGATGAAAAGTATT+CGG + chr8_1:65589355-65589374 Msa1207810:intron 25.0%
!!! AGTTTGAAGAAATTTTTAGC+AGG - chr8_1:65589579-65589598 None:intergenic 25.0%
! CAAAATAAGTAAAATGTTCG+CGG - chr8_1:65589627-65589646 None:intergenic 25.0%
! AAAATAAAAAAATCATGGCG+TGG - chr8_1:65589678-65589697 None:intergenic 25.0%
!!! GTCACTACTTTTAGATAATA+AGG - chr8_1:65589855-65589874 None:intergenic 25.0%
! TGCAATGAAATACTTGAATA+TGG - chr8_1:65590094-65590113 None:intergenic 25.0%
!!! TTGCATTAGTTTTATATGGT+TGG + chr8_1:65590109-65590128 Msa1207810:intron 25.0%
! AAAAATGCACACATATAACT+CGG - chr8_1:65590202-65590221 None:intergenic 25.0%
!!! GCTGTTTTTGTTTTTGTTTT+TGG - chr8_1:65590297-65590316 None:intergenic 25.0%
!! TGAAGTGTTTGATAAATTAG+CGG + chr8_1:65590634-65590653 Msa1207810:intron 25.0%
!!! TACGTCATTTTACATTTATC+AGG - chr8_1:65590668-65590687 None:intergenic 25.0%
!! GAAAATTTTGTAACATCATG+AGG + chr8_1:65590807-65590826 Msa1207810:intron 25.0%
! ATTTGTGTTATCGAAAGAAT+TGG + chr8_1:65590880-65590899 Msa1207810:intron 25.0%
! AAGAAATATCAAATTGGATC+TGG + chr8_1:65590914-65590933 Msa1207810:intron 25.0%
! AGAAATATCAAATTGGATCT+GGG + chr8_1:65590915-65590934 Msa1207810:intron 25.0%
! TTTGCTTGTGTAAAATATAG+TGG + chr8_1:65590960-65590979 Msa1207810:intron 25.0%
! GCCAAAAGAAATAATCAAAT+TGG + chr8_1:65591025-65591044 Msa1207810:intron 25.0%
! CATAAATAATTCCAATCCAA+TGG + chr8_1:65591064-65591083 Msa1207810:intron 25.0%
! AATCAATCCAATGGTTAATA+TGG + chr8_1:65591111-65591130 Msa1207810:intron 25.0%
! TACATCATAAAGGATAAACT+TGG + chr8_1:65591176-65591195 Msa1207810:intron 25.0%
! TATATTTAAGAGTTTAGGAG+AGG - chr8_1:65591447-65591466 Msa1207820:intron 25.0%
!! CTTTTTCTATTGGAAATTAG+TGG - chr8_1:65591486-65591505 Msa1207820:intron 25.0%
!!! TATTGCATTAATTCTAGTTC+TGG - chr8_1:65592235-65592254 Msa1207820:intron 25.0%
! TATATATGTAGGAACATTTC+CGG - chr8_1:65592959-65592978 Msa1207820:intron 25.0%
GAAAAGCTTATCTCATGAAA+TGG - chr8_1:65586312-65586331 None:intergenic 30.0%
AGTTAAATTGTTGTATCCTC+GGG - chr8_1:65586576-65586595 None:intergenic 30.0%
AAAAAAGCAGCACTAAAGAA+AGG - chr8_1:65586645-65586664 None:intergenic 30.0%
TTACCATGCTTATATATCTG+AGG + chr8_1:65586742-65586761 Msa1207810:five_prime_UTR 30.0%
AATCCTCAGATATATAAGCA+TGG - chr8_1:65586748-65586767 None:intergenic 30.0%
ACAGTAAAGAAGAGAAACTA+AGG - chr8_1:65586930-65586949 None:intergenic 30.0%
TTAATGATCATCCACAATTC+TGG - chr8_1:65586995-65587014 None:intergenic 30.0%
!! CCTTTAGTTGTCTTTAGTTT+TGG - chr8_1:65587065-65587084 None:intergenic 30.0%
CCAAAACTAAAGACAACTAA+AGG + chr8_1:65587062-65587081 Msa1207810:intron 30.0%
! AAATAGTTAGCTGTTGACTA+AGG - chr8_1:65587100-65587119 None:intergenic 30.0%
ACAAGACTTTGATCTATGTT+CGG + chr8_1:65587156-65587175 Msa1207810:intron 30.0%
TTTACAATGTAATTATGGGG+AGG - chr8_1:65587472-65587491 None:intergenic 30.0%
AGGTTACAAAACAAAGTAGA+GGG - chr8_1:65587504-65587523 None:intergenic 30.0%
TAGGTTACAAAACAAAGTAG+AGG - chr8_1:65587505-65587524 None:intergenic 30.0%
!!! CTCTTCCTATTTTTATTTGG+AGG + chr8_1:65587618-65587637 Msa1207810:intron 30.0%
!! GATAACACAACATTTGCTAT+TGG + chr8_1:65588234-65588253 Msa1207810:intron 30.0%
!! AAAGATAAGAAGTATCCAAC+AGG + chr8_1:65588327-65588346 Msa1207810:five_prime_UTR 30.0%
ATACTCAAAACAATTCTGGT+TGG + chr8_1:65588413-65588432 Msa1207810:five_prime_UTR 30.0%
! TACTCAAAACAATTCTGGTT+GGG + chr8_1:65588414-65588433 Msa1207810:five_prime_UTR 30.0%
AATCAGATTTCTGTCCATTT+GGG - chr8_1:65588475-65588494 None:intergenic 30.0%
CGTCTTGAAACTAATCAAAA+TGG + chr8_1:65588512-65588531 Msa1207810:CDS 30.0%
!!! ACCATTTTGCTTTAAGAACA+AGG + chr8_1:65588640-65588659 Msa1207810:intron 30.0%
GAAAAAATGGGTACTAAAGA+TGG - chr8_1:65588884-65588903 None:intergenic 30.0%
AAGGGAAGAGATGAAAAAAT+GGG - chr8_1:65588896-65588915 None:intergenic 30.0%
TAAGGGAAGAGATGAAAAAA+TGG - chr8_1:65588897-65588916 None:intergenic 30.0%
!! CAACATTTAGGTTTTGTAGA+GGG - chr8_1:65589063-65589082 None:intergenic 30.0%
!! ACAACATTTAGGTTTTGTAG+AGG - chr8_1:65589064-65589083 None:intergenic 30.0%
ATATGAATCAAAAGTCGTGA+CGG - chr8_1:65589087-65589106 None:intergenic 30.0%
!!! CTTGTTTTTAAGTCAACTCA+TGG - chr8_1:65589329-65589348 None:intergenic 30.0%
TCACATATTCTTCATACGTT+TGG - chr8_1:65589913-65589932 None:intergenic 30.0%
GAGACCAGAATACAAATTTA+TGG + chr8_1:65590025-65590044 Msa1207810:intron 30.0%
AAAATGCACACATATAACTC+GGG - chr8_1:65590201-65590220 None:intergenic 30.0%
!!! TTTTTTATTTCGGCTCTGTT+TGG + chr8_1:65590500-65590519 Msa1207810:intron 30.0%
ATGTGTGTTATCGAAAGAAT+TGG + chr8_1:65590996-65591015 Msa1207810:intron 30.0%
!! TTTTAAGGAAACCATTGGAT+TGG - chr8_1:65591078-65591097 Msa1207820:intron 30.0%
AATATTAAGGAAACGGATCT+GGG - chr8_1:65591155-65591174 Msa1207820:intron 30.0%
AAATATTAAGGAAACGGATC+TGG - chr8_1:65591156-65591175 Msa1207820:intron 30.0%
TGAAATAAGCTATAAGCTCA+TGG + chr8_1:65591307-65591326 Msa1207810:intron 30.0%
TTGACAAAAAAAGCTCTGTT+TGG - chr8_1:65591346-65591365 Msa1207820:intron 30.0%
TCAATTCAACAATTTGATGC+TGG + chr8_1:65591525-65591544 Msa1207810:intron 30.0%
CAATTCAACAATTTGATGCT+GGG + chr8_1:65591526-65591545 Msa1207810:intron 30.0%
TGGGAACAAAAAACAATAAG+AGG + chr8_1:65591545-65591564 Msa1207810:intron 30.0%
TGAAAGATTGTGGATATGTA+GGG - chr8_1:65591609-65591628 Msa1207820:intron 30.0%
TTGAAAGATTGTGGATATGT+AGG - chr8_1:65591610-65591629 Msa1207820:intron 30.0%
!!! TTTTGGCTTGAAAATTCCAA+CGG - chr8_1:65591655-65591674 Msa1207820:intron 30.0%
! GTTCATAGGATTTGCAATTT+TGG - chr8_1:65591672-65591691 Msa1207820:intron 30.0%
GAGTAAAAATCGTAGTTCAT+AGG - chr8_1:65591686-65591705 Msa1207820:intron 30.0%
TCAAAAGGTTATTATCGTTG+TGG + chr8_1:65591760-65591779 Msa1207810:intron 30.0%
TCAAATTAGACATTACGTTC+AGG - chr8_1:65591848-65591867 Msa1207820:intron 30.0%
!! TGTGTATACTTTCTGCTTTT+AGG + chr8_1:65591936-65591955 Msa1207810:intron 30.0%
! ACTATTATAACTTGTACTGC+AGG + chr8_1:65592027-65592046 Msa1207810:intron 30.0%
CATGAGCATGATGATTATAA+GGG - chr8_1:65592339-65592358 Msa1207820:intron 30.0%
CAAAATCTTCACTCACTTTA+TGG + chr8_1:65592430-65592449 Msa1207810:CDS 30.0%
CAAATCACTCTTCTTGATAA+TGG + chr8_1:65592631-65592650 Msa1207810:CDS 30.0%
! CTCAGAAACTTCATTCTTTT+TGG - chr8_1:65592659-65592678 Msa1207820:CDS 30.0%
CAAAAAGAATGAAGTTTCTG+AGG + chr8_1:65592657-65592676 Msa1207810:CDS 30.0%
TGTTCATATATATGAACCTC+TGG + chr8_1:65592866-65592885 Msa1207810:three_prime_UTR 30.0%
AAGCTTATCTCATGAAATGG+TGG - chr8_1:65586309-65586328 None:intergenic 35.0%
! TAAGTTTGGAGCTAGATTTC+AGG - chr8_1:65586334-65586353 None:intergenic 35.0%
GAAAAGGTAAGTTTCAAGAG+TGG - chr8_1:65586429-65586448 None:intergenic 35.0%
ATGAAAGTGAAGGAAGGAAA+AGG - chr8_1:65586445-65586464 None:intergenic 35.0%
AACAGACAGGAAATGAAAAG+TGG - chr8_1:65586601-65586620 None:intergenic 35.0%
!!! GCTTTTTTGTCTGTTTCTGT+TGG + chr8_1:65586657-65586676 Msa1207810:five_prime_UTR 35.0%
!!! ATCGTTTTGAAGTAGTAGCT+TGG - chr8_1:65586689-65586708 None:intergenic 35.0%
AGGATTTAGTGCTACAAACA+AGG + chr8_1:65586762-65586781 Msa1207810:five_prime_UTR 35.0%
ATCCACACTTGAATTCTACT+TGG + chr8_1:65586795-65586814 Msa1207810:five_prime_UTR 35.0%
TGCATCCACACTTAATATCA+AGG + chr8_1:65586824-65586843 Msa1207810:five_prime_UTR 35.0%
GGCATGAAAAGCTAAATGTT+TGG + chr8_1:65587405-65587424 Msa1207810:intron 35.0%
AATGTAATTATGGGGAGGAA+GGG - chr8_1:65587467-65587486 None:intergenic 35.0%
GTTACAAAACAAAGTAGAGG+GGG - chr8_1:65587502-65587521 None:intergenic 35.0%
GGTTACAAAACAAAGTAGAG+GGG - chr8_1:65587503-65587522 None:intergenic 35.0%
CCACTCCTCCAAATAAAAAT+AGG - chr8_1:65587626-65587645 None:intergenic 35.0%
!!! CCTATTTTTATTTGGAGGAG+TGG + chr8_1:65587623-65587642 Msa1207810:intron 35.0%
!!! TGCAGGTTTTAATTCTTCTG+AGG + chr8_1:65587832-65587851 Msa1207810:intron 35.0%
!!! AGGTTTTAATTCTTCTGAGG+TGG + chr8_1:65587835-65587854 Msa1207810:intron 35.0%
TCAACAAGTTCTTCATCAGT+TGG - chr8_1:65587906-65587925 None:intergenic 35.0%
!! AACTTGTTGATTACTACCTC+AGG + chr8_1:65587916-65587935 Msa1207810:five_prime_UTR 35.0%
GAAGTGATGAACAAAATGAC+TGG + chr8_1:65588289-65588308 Msa1207810:five_prime_UTR 35.0%
ATCAGATTTCTGTCCATTTG+GGG - chr8_1:65588474-65588493 None:intergenic 35.0%
CAATCAGATTTCTGTCCATT+TGG - chr8_1:65588476-65588495 None:intergenic 35.0%
CAAATGGACAGAAATCTGAT+TGG + chr8_1:65588474-65588493 Msa1207810:CDS 35.0%
! GCAAAATGGTACATTTCTAG+AGG - chr8_1:65588630-65588649 None:intergenic 35.0%
! GCCTTGTTCTTAAAGCAAAA+TGG - chr8_1:65588644-65588663 None:intergenic 35.0%
!! TTGTGATGAAAAGTATTCGG+AGG + chr8_1:65589358-65589377 Msa1207810:intron 35.0%
GCTCTGTTTGGTAAAAATAG+CGG + chr8_1:65590512-65590531 Msa1207810:intron 35.0%
AGCTTATAGCTGATAGCTTA+TGG + chr8_1:65590584-65590603 Msa1207810:intron 35.0%
GTGTTATCGAAAGAATTGGA+AGG + chr8_1:65590884-65590903 Msa1207810:intron 35.0%
CAATCCAATGGTTAATATGG+CGG + chr8_1:65591114-65591133 Msa1207810:intron 35.0%
TTTAAGAGTTTAGGAGAGGA+GGG - chr8_1:65591443-65591462 Msa1207820:intron 35.0%
ATTTAAGAGTTTAGGAGAGG+AGG - chr8_1:65591444-65591463 Msa1207820:intron 35.0%
!!! CCTTTTGAGACATGTATTGA+GGG - chr8_1:65591748-65591767 Msa1207820:intron 35.0%
!!! ACCTTTTGAGACATGTATTG+AGG - chr8_1:65591749-65591768 Msa1207820:intron 35.0%
ATGATCAATCACCATGTAAC+TGG + chr8_1:65592116-65592135 Msa1207810:CDS 35.0%
TGATCATCATACCAGTTACA+TGG - chr8_1:65592130-65592149 Msa1207820:intron 35.0%
GAGTGGTATGAAGTATAGTT+GGG - chr8_1:65592205-65592224 Msa1207820:intron 35.0%
TGAGTGGTATGAAGTATAGT+TGG - chr8_1:65592206-65592225 Msa1207820:intron 35.0%
GAAAACTAGCATCATGGTAT+TGG - chr8_1:65592270-65592289 Msa1207820:intron 35.0%
!! GAGTAATTTTTGGGCTTTCA+AGG - chr8_1:65592306-65592325 Msa1207820:intron 35.0%
GCATGAGCATGATGATTATA+AGG - chr8_1:65592340-65592359 Msa1207820:intron 35.0%
! GTGAGTGAAGATTTTGTTGA+TGG - chr8_1:65592426-65592445 Msa1207820:intron 35.0%
AATCTTCACTCACTTTATGG+TGG + chr8_1:65592433-65592452 Msa1207810:CDS 35.0%
ATGATGAACAGGTTACTGAT+TGG + chr8_1:65592473-65592492 Msa1207810:CDS 35.0%
GCTTATTATGTACACCAACT+TGG - chr8_1:65592811-65592830 Msa1207820:intron 35.0%
ATGAACAGCTTACAGCATTA+AGG - chr8_1:65592853-65592872 Msa1207820:intron 35.0%
TATATATATCCAGACTCCAG+AGG - chr8_1:65592885-65592904 Msa1207820:intron 35.0%
! ATGAAATGGTGGCGCTAAAA+CGG - chr8_1:65586298-65586317 None:intergenic 40.0%
GAAGAGATGAAAGTGAAGGA+AGG - chr8_1:65586451-65586470 None:intergenic 40.0%
AGGAGAAGAGATGAAAGTGA+AGG - chr8_1:65586455-65586474 None:intergenic 40.0%
!!! AGGTTTTAGTTCAATTCCCG+AGG + chr8_1:65586557-65586576 Msa1207810:five_prime_UTR 40.0%
AGAAAGGCAGAAAGACACAA+GGG - chr8_1:65586629-65586648 None:intergenic 40.0%
AAGAAAGGCAGAAAGACACA+AGG - chr8_1:65586630-65586649 None:intergenic 40.0%
CTACTACTTCAAAACGATCG+TGG + chr8_1:65586691-65586710 Msa1207810:five_prime_UTR 40.0%
CTCCAAGTAGAATTCAAGTG+TGG - chr8_1:65586800-65586819 None:intergenic 40.0%
GACTTTGATCTATGTTCGGT+TGG + chr8_1:65587160-65587179 Msa1207810:intron 40.0%
TCAAATGAATGATGCTCTGC+AGG - chr8_1:65587438-65587457 None:intergenic 40.0%
CAATGTAATTATGGGGAGGA+AGG - chr8_1:65587468-65587487 None:intergenic 40.0%
! ACATTTTCACATGTACCTCC+AGG + chr8_1:65587867-65587886 Msa1207810:five_prime_UTR 40.0%
TGATTACTACCTCAGGAAGA+AGG + chr8_1:65587923-65587942 Msa1207810:five_prime_UTR 40.0%
ATTGAACCATGGGATCTTCA+AGG + chr8_1:65587999-65588018 Msa1207810:five_prime_UTR 40.0%
! TACTAGGAGTACAAGGAGTA+CGG + chr8_1:65588104-65588123 Msa1207810:intron 40.0%
!! CTATTCGTTCTAGTTCCTGT+TGG - chr8_1:65588345-65588364 None:intergenic 40.0%
ACGAATAGAGCAACAAAAGC+AGG + chr8_1:65588357-65588376 Msa1207810:five_prime_UTR 40.0%
! CGCGGAGTACAATTGATAAT+TGG - chr8_1:65589609-65589628 None:intergenic 40.0%
! ATATAACTCGGGTTCGAGTT+CGG - chr8_1:65590190-65590209 None:intergenic 40.0%
CTATAAGCTCAAACGCTACT+TGG + chr8_1:65591253-65591272 Msa1207810:intron 40.0%
AAATCGAGGAAGAGACTGTT+TGG - chr8_1:65591413-65591432 Msa1207820:intron 40.0%
AGGAGGGATTTCAAAAATCG+AGG - chr8_1:65591427-65591446 Msa1207820:intron 40.0%
CCCTCAATACATGTCTCAAA+AGG + chr8_1:65591745-65591764 Msa1207810:intron 40.0%
! TAACTTGTACTGCAGGAAGA+AGG + chr8_1:65592034-65592053 Msa1207810:intron 40.0%
GGTATGAAGTATAGTTGGGA+GGG - chr8_1:65592201-65592220 Msa1207820:intron 40.0%
TGGTATGAAGTATAGTTGGG+AGG - chr8_1:65592202-65592221 Msa1207820:intron 40.0%
!! ACTAGCATCATGGTATTGGT+GGG - chr8_1:65592266-65592285 Msa1207820:intron 40.0%
! AACTAGCATCATGGTATTGG+TGG - chr8_1:65592267-65592286 Msa1207820:intron 40.0%
GTTGGAGAAAACTAGCATCA+TGG - chr8_1:65592276-65592295 Msa1207820:intron 40.0%
!! ATTTTTGGGCTTTCAAGGTG+TGG - chr8_1:65592301-65592320 Msa1207820:intron 40.0%
TATGGTGGTAACAGCAGTAA+TGG + chr8_1:65592448-65592467 Msa1207810:CDS 40.0%
CAGTAATGGCAATGATGAAC+AGG + chr8_1:65592462-65592481 Msa1207810:CDS 40.0%
CACATGAAGAATAGCTGCAT+TGG - chr8_1:65592605-65592624 Msa1207820:intron 40.0%
CAATGCAGCTATTCTTCATG+TGG + chr8_1:65592603-65592622 Msa1207810:CDS 40.0%
! TGATGAGTATTGCTCCAAGT+TGG + chr8_1:65592794-65592813 Msa1207810:three_prime_UTR 40.0%
!! ATATATGAACCTCTGGAGTC+TGG + chr8_1:65592873-65592892 Msa1207810:three_prime_UTR 40.0%
! CTCCTTTTCAGTACTCCTCT+CGG + chr8_1:65586470-65586489 Msa1207810:five_prime_UTR 45.0%
! TCCTTTTCAGTACTCCTCTC+GGG + chr8_1:65586471-65586490 Msa1207810:five_prime_UTR 45.0%
CTTTGTTCTCTCCAACGAGA+GGG - chr8_1:65586534-65586553 None:intergenic 45.0%
TCTTTGTTCTCTCCAACGAG+AGG - chr8_1:65586535-65586554 None:intergenic 45.0%
CGTTGGAGAGAACAAAGAAG+AGG + chr8_1:65586537-65586556 Msa1207810:five_prime_UTR 45.0%
GAAAGGCAGAAAGACACAAG+GGG - chr8_1:65586628-65586647 None:intergenic 45.0%
GAATGATGCTCTGCAGGTAT+AGG - chr8_1:65587432-65587451 None:intergenic 45.0%
GTTGGATGAAAACGAAAGCC+TGG - chr8_1:65587888-65587907 None:intergenic 45.0%
TAGACGATACCTTCTTCCTG+AGG - chr8_1:65587935-65587954 None:intergenic 45.0%
TCGATACCTTGAAGATCCCA+TGG - chr8_1:65588008-65588027 None:intergenic 45.0%
GAGCAACAAAAGCAGGATTC+TGG + chr8_1:65588364-65588383 Msa1207810:five_prime_UTR 45.0%
TATTGCCTTGTCTCTTCCTG+TGG - chr8_1:65588394-65588413 None:intergenic 45.0%
!! AGTCGTGACGGACAACATTT+AGG - chr8_1:65589075-65589094 None:intergenic 45.0%
! TATAACTCGGGTTCGAGTTC+GGG - chr8_1:65590189-65590208 None:intergenic 45.0%
ACTGCCGCCATATTAACCAT+TGG - chr8_1:65591121-65591140 Msa1207820:intron 45.0%
AATTCCAACGGTGGTTGAGA+GGG - chr8_1:65591643-65591662 Msa1207820:intron 45.0%
AAATTCCAACGGTGGTTGAG+AGG - chr8_1:65591644-65591663 Msa1207820:intron 45.0%
TGGCTTGAAAATTCCAACGG+TGG - chr8_1:65591652-65591671 Msa1207820:intron 45.0%
TTGTACTGCAGGAAGAAGGA+TGG + chr8_1:65592038-65592057 Msa1207810:intron 45.0%
TGTACTGCAGGAAGAAGGAT+GGG + chr8_1:65592039-65592058 Msa1207810:intron 45.0%
!! AAGAAGGATGGGTTGTATGC+AGG + chr8_1:65592050-65592069 Msa1207810:CDS 45.0%
!! AGAAGGATGGGTTGTATGCA+GGG + chr8_1:65592051-65592070 Msa1207810:CDS 45.0%
GGATTCAAGATCTGGCATGA+AGG - chr8_1:65592158-65592177 Msa1207820:intron 45.0%
ATAGTTGGGAGGGTGAGAAT+TGG - chr8_1:65592191-65592210 Msa1207820:intron 45.0%
!! CTAGTTCTGGTTTGCATGAG+TGG - chr8_1:65592222-65592241 Msa1207820:intron 45.0%
!! CTAGCATCATGGTATTGGTG+GGG - chr8_1:65592265-65592284 Msa1207820:intron 45.0%
TTCACTTCCAGAGTTCCATG+TGG - chr8_1:65592711-65592730 Msa1207820:intron 45.0%
!!! GTTAAAATATTATTTTTATT+TGG + chr8_1:65586874-65586893 Msa1207810:intron 5.0%
!! ACAAAAAAAAATATTAAATA+TGG - chr8_1:65587333-65587352 None:intergenic 5.0%
!! TAAAAAAAAATAATTAACAA+AGG - chr8_1:65587808-65587827 None:intergenic 5.0%
!!! GTTAAAAAAAATTTTTTTTT+TGG + chr8_1:65589247-65589266 Msa1207810:intron 5.0%
!! TATTAAAATTAAAATACATT+TGG - chr8_1:65592914-65592933 Msa1207820:intron 5.0%
!! TCCCGAGAGGAGTACTGAAA+AGG - chr8_1:65586475-65586494 None:intergenic 50.0%
CACAAGGGGACAAAACAGAC+AGG - chr8_1:65586614-65586633 None:intergenic 50.0%
GGATGAAAACGAAAGCCTGG+AGG - chr8_1:65587885-65587904 None:intergenic 50.0%
GAAAGCCACAGGAAGAGACA+AGG + chr8_1:65588386-65588405 Msa1207810:five_prime_UTR 50.0%
TACAAAGGACGTGCCCCAAA+TGG + chr8_1:65588458-65588477 Msa1207810:CDS 50.0%
TCATGGCGTGGAGAAGCAAT+GGG - chr8_1:65589666-65589685 None:intergenic 50.0%
ATCATGGCGTGGAGAAGCAA+TGG - chr8_1:65589667-65589686 None:intergenic 50.0%
! ATAACTCGGGTTCGAGTTCG+GGG - chr8_1:65590188-65590207 None:intergenic 50.0%
ATTCCAACGGTGGTTGAGAG+GGG - chr8_1:65591642-65591661 Msa1207820:intron 50.0%
!! GTGAGAATTGGTTCGGGTTG+GGG - chr8_1:65592179-65592198 Msa1207820:intron 50.0%
!! GGTGAGAATTGGTTCGGGTT+GGG - chr8_1:65592180-65592199 Msa1207820:intron 50.0%
TGGGAGGGTGAGAATTGGTT+CGG - chr8_1:65592186-65592205 Msa1207820:intron 50.0%
GGCTTTCAAGGTGTGGAAGT+TGG - chr8_1:65592294-65592313 Msa1207820:intron 50.0%
TTCTACCTCCAGTTGCCACA+TGG + chr8_1:65592693-65592712 Msa1207810:CDS 50.0%
GGCAATCGATGATCAGTTGC+CGG + chr8_1:65592937-65592956 Msa1207810:three_prime_UTR 50.0%
GAGAGAGATCAGATCCCGAG+AGG - chr8_1:65586488-65586507 None:intergenic 55.0%
TCTCTCCAACGAGAGGGAGA+GGG - chr8_1:65586528-65586547 None:intergenic 55.0%
TTCTCTCCAACGAGAGGGAG+AGG - chr8_1:65586529-65586548 None:intergenic 55.0%
! GCAGGATTCTGGAAAGCCAC+AGG + chr8_1:65588375-65588394 Msa1207810:five_prime_UTR 55.0%
TTTCCCCTCTCAACCACCGT+TGG + chr8_1:65591636-65591655 Msa1207810:intron 55.0%
! CGGGTTGGGGATTCAAGATC+TGG - chr8_1:65592166-65592185 Msa1207820:intron 55.0%
!! GGGTGAGAATTGGTTCGGGT+TGG - chr8_1:65592181-65592200 Msa1207820:intron 55.0%
! GGGAGGGTGAGAATTGGTTC+GGG - chr8_1:65592185-65592204 Msa1207820:intron 55.0%
GAGTTCCATGTGGCAACTGG+AGG - chr8_1:65592701-65592720 Msa1207820:intron 55.0%
CCAGAGTTCCATGTGGCAAC+TGG - chr8_1:65592704-65592723 Msa1207820:intron 55.0%
CCAGTTGCCACATGGAACTC+TGG + chr8_1:65592701-65592720 Msa1207810:CDS 55.0%
TCTCCAACGAGAGGGAGAGG+GGG - chr8_1:65586526-65586545 None:intergenic 60.0%
CTCTCCAACGAGAGGGAGAG+GGG - chr8_1:65586527-65586546 None:intergenic 60.0%
TCTCCCCCTCTCCCTCTCGT+TGG + chr8_1:65586520-65586539 Msa1207810:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 65586261 65592992 65586261 ID=Msa1207810;Name=Msa1207810
chr8_1 mRNA 65586261 65592992 65586261 ID=Msa1207810-mRNA-1;Parent=Msa1207810;Name=Msa1207810-mRNA-1;_AED=0.06;_eAED=0.06;_QI=932|1|1|1|0.66|0.25|4|263|262
chr8_1 exon 65586261 65586845 65586261 ID=Msa1207810-mRNA-1:exon:21519;Parent=Msa1207810-mRNA-1
chr8_1 exon 65587837 65588020 65587837 ID=Msa1207810-mRNA-1:exon:21520;Parent=Msa1207810-mRNA-1
chr8_1 exon 65588274 65588544 65588274 ID=Msa1207810-mRNA-1:exon:21521;Parent=Msa1207810-mRNA-1
chr8_1 exon 65592049 65592992 65592049 ID=Msa1207810-mRNA-1:exon:21522;Parent=Msa1207810-mRNA-1
chr8_1 five_prime_UTR 65586261 65586845 65586261 ID=Msa1207810-mRNA-1:five_prime_utr;Parent=Msa1207810-mRNA-1
chr8_1 five_prime_UTR 65587837 65588020 65587837 ID=Msa1207810-mRNA-1:five_prime_utr;Parent=Msa1207810-mRNA-1
chr8_1 five_prime_UTR 65588274 65588436 65588274 ID=Msa1207810-mRNA-1:five_prime_utr;Parent=Msa1207810-mRNA-1
chr8_1 CDS 65588437 65588544 65588437 ID=Msa1207810-mRNA-1:cds;Parent=Msa1207810-mRNA-1
chr8_1 CDS 65592049 65592729 65592049 ID=Msa1207810-mRNA-1:cds;Parent=Msa1207810-mRNA-1
chr8_1 three_prime_UTR 65592730 65592992 65592730 ID=Msa1207810-mRNA-1:three_prime_utr;Parent=Msa1207810-mRNA-1
Gene Sequence

>Msa1207810

ATGAGAAAGACTCTAGTATTTTACAAAGGACGTGCCCCAAATGGACAGAAATCTGATTGGATCATGCATGAGTACCGTCTTGAAACTAATCAAAATGGAATTTCTCAGGAAGAAGGATGGGTTGTATGCAGGGTATTCAAGAAAAGAATGACAACAGTGCAGAAAATGAATGAGTATGATCAATCACCATGTAACTGGTATGATGATCAAGTTTCCTTCATGCCAGATCTTGAATCCCCAACCCGAACCAATTCTCACCCTCCCAACTATACTTCATACCACTCATGCAAACCAGAACTAGAATTAATGCAATATAACACACCCCACCAATACCATGATGCTAGTTTTCTCCAACTTCCACACCTTGAAAGCCCAAAAATTACTCAATCTATTATCCCTTATAATCATCATGCTCATGCAAACAATAACAATATTGCACTTACTCAACATCAGCAACAACAACAGATGCAATATTTTCAGTACCATCAACAAAATCTTCACTCACTTTATGGTGGTAACAGCAGTAATGGCAATGATGAACAGGTTACTGATTGGAGAGTGCTTGACAAGTTTGTTGCTTCTCAGCTAAGTCATGAAGAAAATAATAATAATAATAATAATAATCAAGATCATGTTTCTAATAAAGAAGCTAGCTCTTTCTCCAATGCAGCTATTCTTCATGTGGCTGAACAAATCACTCTTCTTGATAATGGATCCAAAAAGAATGAAGTTTCTGAGGAACATGCTTCAACTTCTACCTCCAGTTGCCACATGGAACTCTGGAAGTGA
Protein sequence

>Msa1207810

MRKTLVFYKGRAPNGQKSDWIMHEYRLETNQNGISQEEGWVVCRVFKKRMTTVQKMNEYDQSPCNWYDDQVSFMPDLESPTRTNSHPPNYTSYHSCKPELELMQYNTPHQYHDASFLQLPHLESPKITQSIIPYNHHAHANNNNIALTQHQQQQQMQYFQYHQQNLHSLYGGNSSNGNDEQVTDWRVLDKFVASQLSHEENNNNNNNNQDHVSNKEASSFSNAAILHVAEQITLLDNGSKKNEVSEEHASTSTSSCHMELWK*