Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1211330 | sp|Q9STS6|LBD27_ARATH | 39.216 | 306 | 138 | 8 | 1 | 262 | 27 | 328 | 3.43e-55 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1211330 | A0A072URD2 | 98.997 | 299 | 3 | 0 | 1 | 299 | 1 | 299 | 0.0 | 620 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1211330 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1211330 | MtrunA17_Chr4g0059901 | 98.868 | 265 | 3 | 0 | 1 | 265 | 1 | 265 | 0.0 | 553 |
| Msa1211330 | MtrunA17_Chr8g0342351 | 58.333 | 108 | 45 | 0 | 7 | 114 | 9 | 116 | 1.34e-35 | 129 |
| Msa1211330 | MtrunA17_Chr6g0452671 | 51.786 | 112 | 54 | 0 | 4 | 115 | 5 | 116 | 2.01e-35 | 126 |
| Msa1211330 | MtrunA17_Chr6g0452661 | 50.000 | 114 | 56 | 1 | 9 | 121 | 8 | 121 | 1.20e-32 | 120 |
| Msa1211330 | MtrunA17_Chr6g0452691 | 49.138 | 116 | 58 | 1 | 7 | 121 | 6 | 121 | 6.48e-32 | 117 |
| Msa1211330 | MtrunA17_Chr2g0330201 | 51.376 | 109 | 53 | 0 | 6 | 114 | 15 | 123 | 3.95e-31 | 118 |
| Msa1211330 | MtrunA17_Chr5g0439111 | 51.304 | 115 | 55 | 1 | 9 | 122 | 8 | 122 | 1.68e-30 | 114 |
| Msa1211330 | MtrunA17_Chr7g0227511 | 37.580 | 157 | 86 | 4 | 11 | 162 | 12 | 161 | 3.03e-30 | 112 |
| Msa1211330 | MtrunA17_Chr3g0117121 | 50.538 | 93 | 46 | 0 | 11 | 103 | 11 | 103 | 1.49e-28 | 108 |
| Msa1211330 | MtrunA17_Chr4g0058651 | 48.958 | 96 | 49 | 0 | 11 | 106 | 12 | 107 | 1.76e-28 | 108 |
| Msa1211330 | MtrunA17_Chr4g0055441 | 47.664 | 107 | 55 | 1 | 11 | 116 | 3 | 109 | 1.88e-28 | 107 |
| Msa1211330 | MtrunA17_Chr4g0043421 | 48.000 | 100 | 52 | 0 | 4 | 103 | 9 | 108 | 2.78e-28 | 107 |
| Msa1211330 | MtrunA17_Chr5g0437571 | 43.220 | 118 | 66 | 1 | 6 | 123 | 2 | 118 | 9.66e-28 | 105 |
| Msa1211330 | MtrunA17_Chr6g0449651 | 44.545 | 110 | 61 | 0 | 1 | 110 | 58 | 167 | 1.45e-27 | 106 |
| Msa1211330 | MtrunA17_Chr8g0353981 | 38.037 | 163 | 98 | 1 | 11 | 173 | 8 | 167 | 2.36e-27 | 106 |
| Msa1211330 | MtrunA17_Chr6g0449641 | 47.000 | 100 | 53 | 0 | 11 | 110 | 10 | 109 | 2.84e-27 | 103 |
| Msa1211330 | MtrunA17_Chr3g0106101 | 43.478 | 115 | 64 | 1 | 5 | 119 | 2 | 115 | 3.87e-27 | 104 |
| Msa1211330 | MtrunA17_Chr2g0311601 | 46.000 | 100 | 54 | 0 | 4 | 103 | 7 | 106 | 7.81e-27 | 103 |
| Msa1211330 | MtrunA17_Chr3g0114061 | 42.857 | 119 | 67 | 1 | 3 | 121 | 1 | 118 | 1.24e-26 | 102 |
| Msa1211330 | MtrunA17_Chr5g0402611 | 43.363 | 113 | 64 | 0 | 11 | 123 | 9 | 121 | 7.51e-26 | 104 |
| Msa1211330 | MtrunA17_Chr3g0115431 | 45.000 | 100 | 55 | 0 | 11 | 110 | 10 | 109 | 1.13e-24 | 99.0 |
| Msa1211330 | MtrunA17_Chr5g0438091 | 37.705 | 122 | 76 | 0 | 7 | 128 | 36 | 157 | 7.55e-24 | 96.3 |
| Msa1211330 | MtrunA17_Chr4g0040831 | 39.474 | 114 | 62 | 1 | 6 | 119 | 11 | 117 | 7.85e-24 | 96.3 |
| Msa1211330 | MtrunA17_Chr7g0260971 | 41.509 | 106 | 61 | 1 | 6 | 110 | 10 | 115 | 6.44e-23 | 94.4 |
| Msa1211330 | MtrunA17_Chr1g0200201 | 45.161 | 93 | 51 | 0 | 11 | 103 | 73 | 165 | 6.97e-23 | 94.4 |
| Msa1211330 | MtrunA17_Chr3g0097221 | 45.000 | 100 | 55 | 0 | 11 | 110 | 8 | 107 | 1.25e-22 | 95.1 |
| Msa1211330 | MtrunA17_Chr4g0030001 | 43.617 | 94 | 52 | 1 | 11 | 103 | 25 | 118 | 2.16e-22 | 92.0 |
| Msa1211330 | MtrunA17_Chr5g0437401 | 38.318 | 107 | 66 | 0 | 7 | 113 | 8 | 114 | 5.83e-22 | 90.5 |
| Msa1211330 | MtrunA17_Chr5g0444351 | 40.909 | 110 | 64 | 1 | 11 | 120 | 6 | 114 | 9.01e-22 | 89.4 |
| Msa1211330 | MtrunA17_Chr5g0432451 | 43.269 | 104 | 59 | 0 | 7 | 110 | 40 | 143 | 4.38e-21 | 89.7 |
| Msa1211330 | MtrunA17_Chr7g0225111 | 41.000 | 100 | 59 | 0 | 11 | 110 | 6 | 105 | 4.68e-21 | 86.3 |
| Msa1211330 | MtrunA17_Chr3g0113891 | 40.206 | 97 | 58 | 0 | 7 | 103 | 11 | 107 | 5.22e-21 | 88.2 |
| Msa1211330 | MtrunA17_Chr3g0129071 | 38.462 | 104 | 64 | 0 | 7 | 110 | 44 | 147 | 7.86e-21 | 89.0 |
| Msa1211330 | MtrunA17_Chr8g0374591 | 38.462 | 104 | 64 | 0 | 7 | 110 | 5 | 108 | 2.08e-19 | 82.4 |
| Msa1211330 | MtrunA17_Chr6g0460951 | 41.584 | 101 | 58 | 1 | 11 | 110 | 41 | 141 | 2.60e-19 | 84.7 |
| Msa1211330 | MtrunA17_Chr8g0351721 | 40.385 | 104 | 57 | 2 | 5 | 103 | 9 | 112 | 4.78e-19 | 83.6 |
| Msa1211330 | MtrunA17_Chr5g0435641 | 38.384 | 99 | 60 | 1 | 6 | 103 | 3 | 101 | 6.02e-19 | 82.8 |
| Msa1211330 | MtrunA17_Chr8g0351701 | 41.489 | 94 | 54 | 1 | 11 | 103 | 24 | 117 | 1.50e-18 | 82.8 |
| Msa1211330 | MtrunA17_Chr1g0184281 | 41.000 | 100 | 58 | 1 | 11 | 109 | 16 | 115 | 4.39e-18 | 80.9 |
| Msa1211330 | MtrunA17_Chr8g0374601 | 38.235 | 102 | 63 | 0 | 9 | 110 | 25 | 126 | 1.06e-17 | 81.3 |
| Msa1211330 | MtrunA17_Chr1g0184271 | 39.000 | 100 | 60 | 1 | 11 | 109 | 13 | 112 | 1.65e-17 | 80.1 |
| Msa1211330 | MtrunA17_Chr7g0261031 | 37.143 | 105 | 65 | 1 | 6 | 109 | 4 | 108 | 3.31e-17 | 79.0 |
| Msa1211330 | MtrunA17_Chr7g0245871 | 39.362 | 94 | 56 | 1 | 11 | 103 | 28 | 121 | 3.33e-17 | 79.3 |
| Msa1211330 | MtrunA17_Chr6g0460941 | 41.379 | 87 | 50 | 1 | 11 | 96 | 8 | 94 | 2.60e-16 | 73.2 |
| Msa1211330 | MtrunA17_Chr8g0374581 | 38.835 | 103 | 62 | 1 | 9 | 110 | 7 | 109 | 1.25e-15 | 74.7 |
| Msa1211330 | MtrunA17_Chr2g0326041 | 52.083 | 48 | 23 | 0 | 11 | 58 | 34 | 81 | 1.44e-13 | 65.5 |
| Msa1211330 | MtrunA17_Chr4g0046201 | 33.333 | 87 | 58 | 0 | 10 | 96 | 6 | 92 | 4.78e-12 | 65.1 |
| Msa1211330 | MtrunA17_Chr6g0449311 | 33.083 | 133 | 86 | 2 | 38 | 167 | 1 | 133 | 6.84e-12 | 62.4 |
| Msa1211330 | MtrunA17_Chr7g0245861 | 38.202 | 89 | 52 | 2 | 11 | 96 | 13 | 101 | 3.19e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1211330 | AT3G47870.1 | 39.216 | 306 | 138 | 8 | 1 | 262 | 27 | 328 | 3.49e-56 | 184 |
| Msa1211330 | AT3G13850.1 | 46.452 | 155 | 68 | 2 | 9 | 161 | 35 | 176 | 1.16e-35 | 129 |
| Msa1211330 | AT1G72980.1 | 47.287 | 129 | 63 | 1 | 2 | 125 | 5 | 133 | 8.77e-34 | 123 |
| Msa1211330 | AT5G63090.4 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.52e-28 | 108 |
| Msa1211330 | AT5G63090.3 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.52e-28 | 108 |
| Msa1211330 | AT5G63090.2 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.52e-28 | 108 |
| Msa1211330 | AT5G63090.1 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.52e-28 | 108 |
| Msa1211330 | AT1G65620.1 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.10e-27 | 106 |
| Msa1211330 | AT1G65620.4 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.10e-27 | 106 |
| Msa1211330 | AT1G65620.3 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.10e-27 | 106 |
| Msa1211330 | AT1G65620.5 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.10e-27 | 106 |
| Msa1211330 | AT1G65620.2 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.10e-27 | 106 |
| Msa1211330 | AT3G27650.1 | 48.454 | 97 | 50 | 0 | 7 | 103 | 36 | 132 | 1.18e-27 | 105 |
| Msa1211330 | AT2G30130.1 | 41.667 | 120 | 69 | 1 | 3 | 122 | 1 | 119 | 1.98e-26 | 103 |
| Msa1211330 | AT1G31320.1 | 47.312 | 93 | 49 | 0 | 4 | 96 | 7 | 99 | 2.32e-25 | 99.8 |
| Msa1211330 | AT5G66870.1 | 44.231 | 104 | 58 | 0 | 7 | 110 | 4 | 107 | 2.52e-25 | 103 |
| Msa1211330 | AT2G28500.1 | 40.179 | 112 | 65 | 1 | 11 | 122 | 56 | 165 | 1.39e-22 | 94.0 |
| Msa1211330 | AT2G30340.2 | 40.909 | 110 | 63 | 1 | 7 | 116 | 50 | 157 | 1.92e-22 | 94.7 |
| Msa1211330 | AT2G30340.1 | 40.909 | 110 | 63 | 1 | 7 | 116 | 49 | 156 | 2.15e-22 | 94.4 |
| Msa1211330 | AT1G16530.1 | 41.284 | 109 | 61 | 2 | 5 | 112 | 9 | 115 | 2.76e-22 | 91.7 |
| Msa1211330 | AT1G07900.1 | 44.086 | 93 | 52 | 0 | 11 | 103 | 34 | 126 | 2.97e-22 | 92.0 |
| Msa1211330 | AT2G23660.2 | 42.000 | 100 | 58 | 0 | 11 | 110 | 6 | 105 | 7.42e-22 | 93.6 |
| Msa1211330 | AT2G23660.3 | 42.000 | 100 | 58 | 0 | 11 | 110 | 6 | 105 | 7.42e-22 | 93.6 |
| Msa1211330 | AT2G23660.1 | 42.000 | 100 | 58 | 0 | 11 | 110 | 6 | 105 | 7.42e-22 | 93.6 |
| Msa1211330 | AT3G11090.1 | 35.000 | 120 | 78 | 0 | 7 | 126 | 8 | 127 | 5.34e-21 | 88.2 |
| Msa1211330 | AT3G03760.1 | 41.584 | 101 | 58 | 1 | 11 | 110 | 52 | 152 | 1.08e-20 | 89.7 |
| Msa1211330 | AT1G06280.1 | 47.619 | 84 | 44 | 0 | 9 | 92 | 23 | 106 | 2.17e-20 | 87.4 |
| Msa1211330 | AT3G50510.1 | 42.000 | 100 | 58 | 0 | 11 | 110 | 13 | 112 | 2.26e-20 | 87.4 |
| Msa1211330 | AT3G50510.2 | 42.000 | 100 | 58 | 0 | 11 | 110 | 13 | 112 | 2.26e-20 | 87.4 |
| Msa1211330 | AT2G40470.2 | 39.655 | 116 | 70 | 0 | 7 | 122 | 17 | 132 | 5.42e-20 | 86.3 |
| Msa1211330 | AT2G40470.1 | 40.541 | 111 | 66 | 0 | 7 | 117 | 42 | 152 | 1.52e-19 | 85.5 |
| Msa1211330 | AT5G06080.1 | 33.333 | 165 | 103 | 4 | 10 | 170 | 7 | 168 | 4.87e-19 | 83.2 |
| Msa1211330 | AT4G00210.1 | 39.623 | 106 | 63 | 1 | 6 | 110 | 7 | 112 | 5.43e-19 | 84.0 |
| Msa1211330 | AT5G15060.1 | 45.570 | 79 | 41 | 1 | 5 | 81 | 12 | 90 | 7.51e-19 | 81.6 |
| Msa1211330 | AT3G26660.1 | 34.234 | 111 | 73 | 0 | 11 | 121 | 6 | 116 | 1.81e-18 | 80.1 |
| Msa1211330 | AT2G42440.1 | 35.593 | 118 | 68 | 2 | 6 | 122 | 3 | 113 | 3.28e-18 | 82.4 |
| Msa1211330 | AT2G42430.1 | 36.792 | 106 | 66 | 1 | 6 | 110 | 11 | 116 | 3.57e-18 | 82.4 |
| Msa1211330 | AT4G00220.1 | 41.304 | 92 | 53 | 1 | 13 | 103 | 20 | 111 | 5.27e-18 | 81.6 |
| Msa1211330 | AT3G26620.1 | 33.036 | 112 | 75 | 0 | 11 | 122 | 6 | 117 | 5.32e-18 | 78.6 |
| Msa1211330 | AT3G58190.1 | 38.017 | 121 | 67 | 2 | 11 | 130 | 12 | 125 | 5.93e-18 | 81.3 |
| Msa1211330 | AT2G45420.1 | 40.217 | 92 | 54 | 1 | 13 | 103 | 40 | 131 | 8.89e-18 | 81.6 |
| Msa1211330 | AT5G35900.1 | 31.000 | 100 | 69 | 0 | 11 | 110 | 6 | 105 | 1.00e-16 | 77.4 |
| Msa1211330 | AT2G31310.1 | 29.412 | 153 | 94 | 5 | 5 | 144 | 2 | 153 | 2.72e-16 | 75.9 |
| Msa1211330 | AT2G19820.1 | 33.673 | 98 | 59 | 2 | 11 | 107 | 13 | 105 | 7.66e-15 | 70.1 |
| Msa1211330 | AT2G45410.1 | 39.394 | 99 | 59 | 1 | 13 | 110 | 19 | 117 | 9.85e-15 | 71.6 |
Find 55 sgRNAs with CRISPR-Local
Find 158 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGTTCACAAATTATT+TGG | 0.102603 | 8_1:-70925425 | Msa1211330:CDS |
| CAAATAATTTGTGAACATTT+TGG | 0.141519 | 8_1:+70925426 | None:intergenic |
| CAGGTGTGCACTTTCTTCTT+TGG | 0.204290 | 8_1:+70925495 | None:intergenic |
| TTATCATTCAAGCTAATTAT+AGG | 0.238366 | 8_1:-70925334 | Msa1211330:CDS |
| GTGACTTCACAGTTAGAATT+AGG | 0.249617 | 8_1:-70925164 | Msa1211330:CDS |
| ATCAACAACTTGAAATTTGC+AGG | 0.281961 | 8_1:-70925223 | Msa1211330:CDS |
| GGTTGATCTGCAGGGAAATA+AGG | 0.290053 | 8_1:+70925458 | None:intergenic |
| GCAGGCTACAATATCAAATA+TGG | 0.297786 | 8_1:-70925274 | Msa1211330:CDS |
| AGCAATGCAACCTCATAAAT+TGG | 0.298388 | 8_1:-70924904 | Msa1211330:CDS |
| GCAATCATACATTTATTCTA+AGG | 0.300515 | 8_1:-70924784 | Msa1211330:CDS |
| TATCATTCAAGCTAATTATA+GGG | 0.334002 | 8_1:-70925333 | Msa1211330:CDS |
| AATAATAATAATGACCCTTA+AGG | 0.352659 | 8_1:-70925552 | None:intergenic |
| CTTGTGTTGTGCCACCCTTA+AGG | 0.355961 | 8_1:+70925537 | None:intergenic |
| GGGCACTGAAGCCACAAATT+AGG | 0.358880 | 8_1:+70924954 | None:intergenic |
| TTGTGTTGTGCCACCCTTAA+GGG | 0.363348 | 8_1:+70925538 | None:intergenic |
| CAGGGAAATAAGGAGCTAAA+AGG | 0.379418 | 8_1:+70925468 | None:intergenic |
| TTGGAATTTGCATGCTGCAC+AGG | 0.387931 | 8_1:+70925514 | None:intergenic |
| TATCTTCAACAACTCCTTGT+TGG | 0.395214 | 8_1:+70924850 | None:intergenic |
| CTGCTATTGTTATTTGTGTA+AGG | 0.395645 | 8_1:+70924933 | None:intergenic |
| CTTCATGAGGTAACTGTTGT+TGG | 0.400649 | 8_1:+70925189 | None:intergenic |
| ATAATAATAATGACCCTTAA+GGG | 0.421007 | 8_1:-70925551 | None:intergenic |
| TTGTTATAAGTCTGTGGTTG+TGG | 0.430309 | 8_1:+70925095 | None:intergenic |
| ATGCTACAAATCCTAATTTG+TGG | 0.446980 | 8_1:-70924965 | Msa1211330:CDS |
| AAAGAGTGCGAGCGCATTGA+AGG | 0.447294 | 8_1:+70925127 | None:intergenic |
| GATGTTGTGAGGAAATTTGC+AGG | 0.467829 | 8_1:-70925292 | Msa1211330:CDS |
| TCTGAGGTAACCAATTTATG+AGG | 0.490694 | 8_1:+70924894 | None:intergenic |
| ACAATATCAAATATGGATGA+TGG | 0.492612 | 8_1:-70925267 | Msa1211330:CDS |
| TGCTATTGTTATTTGTGTAA+GGG | 0.508900 | 8_1:+70924934 | None:intergenic |
| ATAATCCACACTATTGCTGT+TGG | 0.518450 | 8_1:+70925022 | None:intergenic |
| TAATAACTCCTCCCTCTACA+TGG | 0.525658 | 8_1:-70925000 | Msa1211330:CDS |
| TTGAATGATAATAGAATCCA+TGG | 0.528060 | 8_1:+70925346 | None:intergenic |
| CTCCCTCTACATGGATTCAA+AGG | 0.529912 | 8_1:-70924991 | Msa1211330:CDS |
| ATCATACATTTATTCTAAGG+AGG | 0.554320 | 8_1:-70924781 | Msa1211330:CDS |
| CTGATCAGTTGACACTGGTA+AGG | 0.557706 | 8_1:+70924375 | None:intergenic |
| TTATTGTTGTTATAAGTCTG+TGG | 0.569885 | 8_1:+70925089 | None:intergenic |
| TGTCAAAATATTAAAGTACC+TGG | 0.571068 | 8_1:-70925390 | Msa1211330:CDS |
| TCCTCACAACATCCATGCAC+AGG | 0.573785 | 8_1:+70925302 | None:intergenic |
| CACACTATTGCTGTTGGAGT+AGG | 0.575688 | 8_1:+70925028 | None:intergenic |
| CCTTGTTGGACAGATGATAG+TGG | 0.578651 | 8_1:+70924864 | None:intergenic |
| AGGGATAAGTATCCTGTGCA+TGG | 0.583412 | 8_1:-70925314 | Msa1211330:CDS |
| TTCACAGTTAGAATTAGGAA+TGG | 0.586596 | 8_1:-70925159 | Msa1211330:CDS |
| ATCCTTTGAATCCATGTAGA+GGG | 0.590065 | 8_1:+70924989 | None:intergenic |
| TAGTCAGAAGAAAACAGCCA+TGG | 0.610751 | 8_1:-70925363 | Msa1211330:CDS |
| TAAGGAGCTAAAAGGCACTC+AGG | 0.614015 | 8_1:+70925476 | None:intergenic |
| CTACTCCAACAGCAATAGTG+TGG | 0.620940 | 8_1:-70925027 | Msa1211330:CDS |
| CATCCTTTGAATCCATGTAG+AGG | 0.632560 | 8_1:+70924988 | None:intergenic |
| CCACTATCATCTGTCCAACA+AGG | 0.637452 | 8_1:-70924864 | Msa1211330:CDS |
| TGATAGTGGCTGCGACTCTG+AGG | 0.643276 | 8_1:+70924878 | None:intergenic |
| CTAAGGAGGCTTATGAATCG+AGG | 0.657710 | 8_1:-70924767 | Msa1211330:intron |
| TAGTACTGATCAGTTGACAC+TGG | 0.662425 | 8_1:+70924370 | None:intergenic |
| CTTTGAATCCATGTAGAGGG+AGG | 0.666923 | 8_1:+70924992 | None:intergenic |
| TGTGAAGTCACATCTTCATG+AGG | 0.677402 | 8_1:+70925176 | None:intergenic |
| ATAATAATGACCCTTAAGGG+TGG | 0.680795 | 8_1:-70925548 | None:intergenic |
| TCCTGTGCATGGATGTTGTG+AGG | 0.692042 | 8_1:-70925303 | Msa1211330:CDS |
| TGTTGCTGAGATACAGACAA+AGG | 0.703585 | 8_1:+70925053 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAACATTTTGGAAAATTTT+GGG | + | chr8_1:70924487-70924506 | None:intergenic | 20.0% |
| !!! | CAAATAATTTGTGAACATTT+TGG | + | chr8_1:70924498-70924517 | None:intergenic | 20.0% |
| !! | CAAAATGTTCACAAATTATT+TGG | - | chr8_1:70924496-70924515 | Msa1211330:intron | 20.0% |
| !! | TTATCATTCAAGCTAATTAT+AGG | - | chr8_1:70924587-70924606 | Msa1211330:intron | 20.0% |
| !! | TATCATTCAAGCTAATTATA+GGG | - | chr8_1:70924588-70924607 | Msa1211330:intron | 20.0% |
| !! | AATGTTGAAATTAGATATAG+TGG | - | chr8_1:70925353-70925372 | Msa1211330:CDS | 20.0% |
| !! | ATGTTGAAATTAGATATAGT+GGG | - | chr8_1:70925354-70925373 | Msa1211330:CDS | 20.0% |
| !!! | TGAACATTTTGGAAAATTTT+GGG | + | chr8_1:70924487-70924506 | None:intergenic | 20.0% |
| !!! | CAAATAATTTGTGAACATTT+TGG | + | chr8_1:70924498-70924517 | None:intergenic | 20.0% |
| !! | CAAAATGTTCACAAATTATT+TGG | - | chr8_1:70924496-70924515 | Msa1211330:intron | 20.0% |
| !! | TTATCATTCAAGCTAATTAT+AGG | - | chr8_1:70924587-70924606 | Msa1211330:intron | 20.0% |
| !! | TATCATTCAAGCTAATTATA+GGG | - | chr8_1:70924588-70924607 | Msa1211330:intron | 20.0% |
| !! | AATGTTGAAATTAGATATAG+TGG | - | chr8_1:70925353-70925372 | Msa1211330:CDS | 20.0% |
| !! | ATGTTGAAATTAGATATAGT+GGG | - | chr8_1:70925354-70925373 | Msa1211330:CDS | 20.0% |
| !!! | GTGAACATTTTGGAAAATTT+TGG | + | chr8_1:70924488-70924507 | None:intergenic | 25.0% |
| !! | TGTCAAAATATTAAAGTACC+TGG | - | chr8_1:70924531-70924550 | Msa1211330:intron | 25.0% |
| ! | TTGAATGATAATAGAATCCA+TGG | + | chr8_1:70924578-70924597 | None:intergenic | 25.0% |
| ! | ACAATATCAAATATGGATGA+TGG | - | chr8_1:70924654-70924673 | Msa1211330:intron | 25.0% |
| !! | TTATTGTTGTTATAAGTCTG+TGG | + | chr8_1:70924835-70924854 | None:intergenic | 25.0% |
| !!! | TGCTATTGTTATTTGTGTAA+GGG | + | chr8_1:70924990-70925009 | None:intergenic | 25.0% |
| !!! | TTTTTGATACAGTTGATGAT+AGG | - | chr8_1:70925115-70925134 | Msa1211330:CDS | 25.0% |
| ! | GCAATCATACATTTATTCTA+AGG | - | chr8_1:70925137-70925156 | Msa1211330:CDS | 25.0% |
| !! | ATCATACATTTATTCTAAGG+AGG | - | chr8_1:70925140-70925159 | Msa1211330:CDS | 25.0% |
| !!! | GTGTTTTTGATCTACTTTAA+CGG | + | chr8_1:70925218-70925237 | None:intergenic | 25.0% |
| !!! | GTGAACATTTTGGAAAATTT+TGG | + | chr8_1:70924488-70924507 | None:intergenic | 25.0% |
| !! | TGTCAAAATATTAAAGTACC+TGG | - | chr8_1:70924531-70924550 | Msa1211330:intron | 25.0% |
| ! | TTGAATGATAATAGAATCCA+TGG | + | chr8_1:70924578-70924597 | None:intergenic | 25.0% |
| ! | ACAATATCAAATATGGATGA+TGG | - | chr8_1:70924654-70924673 | Msa1211330:intron | 25.0% |
| !! | TTATTGTTGTTATAAGTCTG+TGG | + | chr8_1:70924835-70924854 | None:intergenic | 25.0% |
| !!! | TGCTATTGTTATTTGTGTAA+GGG | + | chr8_1:70924990-70925009 | None:intergenic | 25.0% |
| !!! | TTTTTGATACAGTTGATGAT+AGG | - | chr8_1:70925115-70925134 | Msa1211330:CDS | 25.0% |
| ! | GCAATCATACATTTATTCTA+AGG | - | chr8_1:70925137-70925156 | Msa1211330:CDS | 25.0% |
| !! | ATCATACATTTATTCTAAGG+AGG | - | chr8_1:70925140-70925159 | Msa1211330:CDS | 25.0% |
| !!! | GTGTTTTTGATCTACTTTAA+CGG | + | chr8_1:70925218-70925237 | None:intergenic | 25.0% |
| ATCAACAACTTGAAATTTGC+AGG | - | chr8_1:70924698-70924717 | Msa1211330:intron | 30.0% | |
| TTCACAGTTAGAATTAGGAA+TGG | - | chr8_1:70924762-70924781 | Msa1211330:intron | 30.0% | |
| ATGCTACAAATCCTAATTTG+TGG | - | chr8_1:70924956-70924975 | Msa1211330:CDS | 30.0% | |
| !! | CTGCTATTGTTATTTGTGTA+AGG | + | chr8_1:70924991-70925010 | None:intergenic | 30.0% |
| ! | TATCAGCATTTTCACTACAA+AGG | + | chr8_1:70925414-70925433 | None:intergenic | 30.0% |
| ATCAACAACTTGAAATTTGC+AGG | - | chr8_1:70924698-70924717 | Msa1211330:intron | 30.0% | |
| TTCACAGTTAGAATTAGGAA+TGG | - | chr8_1:70924762-70924781 | Msa1211330:intron | 30.0% | |
| ATGCTACAAATCCTAATTTG+TGG | - | chr8_1:70924956-70924975 | Msa1211330:CDS | 30.0% | |
| !! | CTGCTATTGTTATTTGTGTA+AGG | + | chr8_1:70924991-70925010 | None:intergenic | 30.0% |
| ! | TATCAGCATTTTCACTACAA+AGG | + | chr8_1:70925414-70925433 | None:intergenic | 30.0% |
| !!! | AAATTTTGGGTTGATCTGCA+GGG | + | chr8_1:70924474-70924493 | None:intergenic | 35.0% |
| !!! | AAAATTTTGGGTTGATCTGC+AGG | + | chr8_1:70924475-70924494 | None:intergenic | 35.0% |
| GCAGGCTACAATATCAAATA+TGG | - | chr8_1:70924647-70924666 | Msa1211330:intron | 35.0% | |
| GTGACTTCACAGTTAGAATT+AGG | - | chr8_1:70924757-70924776 | Msa1211330:intron | 35.0% | |
| TTGTTATAAGTCTGTGGTTG+TGG | + | chr8_1:70924829-70924848 | None:intergenic | 35.0% | |
| ATAATCCACACTATTGCTGT+TGG | + | chr8_1:70924902-70924921 | None:intergenic | 35.0% | |
| ATCCTTTGAATCCATGTAGA+GGG | + | chr8_1:70924935-70924954 | None:intergenic | 35.0% | |
| AGCAATGCAACCTCATAAAT+TGG | - | chr8_1:70925017-70925036 | Msa1211330:CDS | 35.0% | |
| ! | TCTGAGGTAACCAATTTATG+AGG | + | chr8_1:70925030-70925049 | None:intergenic | 35.0% |
| TATCTTCAACAACTCCTTGT+TGG | + | chr8_1:70925074-70925093 | None:intergenic | 35.0% | |
| GCTTATGAATCGAGGTAAAT+TGG | - | chr8_1:70925162-70925181 | Msa1211330:CDS | 35.0% | |
| !!! | AAATTTTGGGTTGATCTGCA+GGG | + | chr8_1:70924474-70924493 | None:intergenic | 35.0% |
| !!! | AAAATTTTGGGTTGATCTGC+AGG | + | chr8_1:70924475-70924494 | None:intergenic | 35.0% |
| GCAGGCTACAATATCAAATA+TGG | - | chr8_1:70924647-70924666 | Msa1211330:intron | 35.0% | |
| GTGACTTCACAGTTAGAATT+AGG | - | chr8_1:70924757-70924776 | Msa1211330:intron | 35.0% | |
| TTGTTATAAGTCTGTGGTTG+TGG | + | chr8_1:70924829-70924848 | None:intergenic | 35.0% | |
| ATAATCCACACTATTGCTGT+TGG | + | chr8_1:70924902-70924921 | None:intergenic | 35.0% | |
| ATCCTTTGAATCCATGTAGA+GGG | + | chr8_1:70924935-70924954 | None:intergenic | 35.0% | |
| AGCAATGCAACCTCATAAAT+TGG | - | chr8_1:70925017-70925036 | Msa1211330:CDS | 35.0% | |
| ! | TCTGAGGTAACCAATTTATG+AGG | + | chr8_1:70925030-70925049 | None:intergenic | 35.0% |
| TATCTTCAACAACTCCTTGT+TGG | + | chr8_1:70925074-70925093 | None:intergenic | 35.0% | |
| GCTTATGAATCGAGGTAAAT+TGG | - | chr8_1:70925162-70925181 | Msa1211330:CDS | 35.0% | |
| CAGGGAAATAAGGAGCTAAA+AGG | + | chr8_1:70924456-70924475 | None:intergenic | 40.0% | |
| ! | TTTTCTTCTGACTAGGTTCC+AGG | + | chr8_1:70924552-70924571 | None:intergenic | 40.0% |
| !! | ATGGCTGTTTTCTTCTGACT+AGG | + | chr8_1:70924559-70924578 | None:intergenic | 40.0% |
| TAGTCAGAAGAAAACAGCCA+TGG | - | chr8_1:70924558-70924577 | Msa1211330:intron | 40.0% | |
| GATGTTGTGAGGAAATTTGC+AGG | - | chr8_1:70924629-70924648 | Msa1211330:intron | 40.0% | |
| CTTCATGAGGTAACTGTTGT+TGG | + | chr8_1:70924735-70924754 | None:intergenic | 40.0% | |
| TGTGAAGTCACATCTTCATG+AGG | + | chr8_1:70924748-70924767 | None:intergenic | 40.0% | |
| TGTTGCTGAGATACAGACAA+AGG | + | chr8_1:70924871-70924890 | None:intergenic | 40.0% | |
| TAATAACTCCTCCCTCTACA+TGG | - | chr8_1:70924921-70924940 | Msa1211330:CDS | 40.0% | |
| CATCCTTTGAATCCATGTAG+AGG | + | chr8_1:70924936-70924955 | None:intergenic | 40.0% | |
| CTAGATGATCATTGATCCAC+AGG | - | chr8_1:70925245-70925264 | Msa1211330:CDS | 40.0% | |
| TCTCATTTAGTACGACCACT+CGG | + | chr8_1:70925323-70925342 | None:intergenic | 40.0% | |
| CAGGGAAATAAGGAGCTAAA+AGG | + | chr8_1:70924456-70924475 | None:intergenic | 40.0% | |
| ! | TTTTCTTCTGACTAGGTTCC+AGG | + | chr8_1:70924552-70924571 | None:intergenic | 40.0% |
| !! | ATGGCTGTTTTCTTCTGACT+AGG | + | chr8_1:70924559-70924578 | None:intergenic | 40.0% |
| TAGTCAGAAGAAAACAGCCA+TGG | - | chr8_1:70924558-70924577 | Msa1211330:intron | 40.0% | |
| GATGTTGTGAGGAAATTTGC+AGG | - | chr8_1:70924629-70924648 | Msa1211330:intron | 40.0% | |
| CTTCATGAGGTAACTGTTGT+TGG | + | chr8_1:70924735-70924754 | None:intergenic | 40.0% | |
| TGTGAAGTCACATCTTCATG+AGG | + | chr8_1:70924748-70924767 | None:intergenic | 40.0% | |
| TGTTGCTGAGATACAGACAA+AGG | + | chr8_1:70924871-70924890 | None:intergenic | 40.0% | |
| TAATAACTCCTCCCTCTACA+TGG | - | chr8_1:70924921-70924940 | Msa1211330:CDS | 40.0% | |
| CATCCTTTGAATCCATGTAG+AGG | + | chr8_1:70924936-70924955 | None:intergenic | 40.0% | |
| CTAGATGATCATTGATCCAC+AGG | - | chr8_1:70925245-70925264 | Msa1211330:CDS | 40.0% | |
| TCTCATTTAGTACGACCACT+CGG | + | chr8_1:70925323-70925342 | None:intergenic | 40.0% | |
| TTGTGTTGTGCCACCCTTAA+GGG | + | chr8_1:70924386-70924405 | None:intergenic | 45.0% | |
| TTGGAATTTGCATGCTGCAC+AGG | + | chr8_1:70924410-70924429 | None:intergenic | 45.0% | |
| CAGGTGTGCACTTTCTTCTT+TGG | + | chr8_1:70924429-70924448 | None:intergenic | 45.0% | |
| !! | TAAGGAGCTAAAAGGCACTC+AGG | + | chr8_1:70924448-70924467 | None:intergenic | 45.0% |
| GGTTGATCTGCAGGGAAATA+AGG | + | chr8_1:70924466-70924485 | None:intergenic | 45.0% | |
| AGGGATAAGTATCCTGTGCA+TGG | - | chr8_1:70924607-70924626 | Msa1211330:intron | 45.0% | |
| ! | CACACTATTGCTGTTGGAGT+AGG | + | chr8_1:70924896-70924915 | None:intergenic | 45.0% |
| CTACTCCAACAGCAATAGTG+TGG | - | chr8_1:70924894-70924913 | Msa1211330:CDS | 45.0% | |
| CTTTGAATCCATGTAGAGGG+AGG | + | chr8_1:70924932-70924951 | None:intergenic | 45.0% | |
| CTCCCTCTACATGGATTCAA+AGG | - | chr8_1:70924930-70924949 | Msa1211330:CDS | 45.0% | |
| CCTTGTTGGACAGATGATAG+TGG | + | chr8_1:70925060-70925079 | None:intergenic | 45.0% | |
| CCACTATCATCTGTCCAACA+AGG | - | chr8_1:70925057-70925076 | Msa1211330:CDS | 45.0% | |
| CTAAGGAGGCTTATGAATCG+AGG | - | chr8_1:70925154-70925173 | Msa1211330:CDS | 45.0% | |
| ACAAGATTACCTCTGGTCGA+GGG | + | chr8_1:70925273-70925292 | None:intergenic | 45.0% | |
| GACCAGAGGTAATCTTGTCA+GGG | - | chr8_1:70925275-70925294 | Msa1211330:CDS | 45.0% | |
| ACCAGAGGTAATCTTGTCAG+GGG | - | chr8_1:70925276-70925295 | Msa1211330:CDS | 45.0% | |
| TCATTTAGTACGACCACTCG+GGG | + | chr8_1:70925321-70925340 | None:intergenic | 45.0% | |
| CTCATTTAGTACGACCACTC+GGG | + | chr8_1:70925322-70925341 | None:intergenic | 45.0% | |
| GTACATTTCTGCCTGCAGTT+CGG | - | chr8_1:70925444-70925463 | Msa1211330:CDS | 45.0% | |
| TTGTGTTGTGCCACCCTTAA+GGG | + | chr8_1:70924386-70924405 | None:intergenic | 45.0% | |
| TTGGAATTTGCATGCTGCAC+AGG | + | chr8_1:70924410-70924429 | None:intergenic | 45.0% | |
| CAGGTGTGCACTTTCTTCTT+TGG | + | chr8_1:70924429-70924448 | None:intergenic | 45.0% | |
| !! | TAAGGAGCTAAAAGGCACTC+AGG | + | chr8_1:70924448-70924467 | None:intergenic | 45.0% |
| GGTTGATCTGCAGGGAAATA+AGG | + | chr8_1:70924466-70924485 | None:intergenic | 45.0% | |
| AGGGATAAGTATCCTGTGCA+TGG | - | chr8_1:70924607-70924626 | Msa1211330:intron | 45.0% | |
| ! | CACACTATTGCTGTTGGAGT+AGG | + | chr8_1:70924896-70924915 | None:intergenic | 45.0% |
| CTACTCCAACAGCAATAGTG+TGG | - | chr8_1:70924894-70924913 | Msa1211330:CDS | 45.0% | |
| CTTTGAATCCATGTAGAGGG+AGG | + | chr8_1:70924932-70924951 | None:intergenic | 45.0% | |
| CTCCCTCTACATGGATTCAA+AGG | - | chr8_1:70924930-70924949 | Msa1211330:CDS | 45.0% | |
| CCTTGTTGGACAGATGATAG+TGG | + | chr8_1:70925060-70925079 | None:intergenic | 45.0% | |
| CCACTATCATCTGTCCAACA+AGG | - | chr8_1:70925057-70925076 | Msa1211330:CDS | 45.0% | |
| CTAAGGAGGCTTATGAATCG+AGG | - | chr8_1:70925154-70925173 | Msa1211330:CDS | 45.0% | |
| ACAAGATTACCTCTGGTCGA+GGG | + | chr8_1:70925273-70925292 | None:intergenic | 45.0% | |
| GACCAGAGGTAATCTTGTCA+GGG | - | chr8_1:70925275-70925294 | Msa1211330:CDS | 45.0% | |
| ACCAGAGGTAATCTTGTCAG+GGG | - | chr8_1:70925276-70925295 | Msa1211330:CDS | 45.0% | |
| TCATTTAGTACGACCACTCG+GGG | + | chr8_1:70925321-70925340 | None:intergenic | 45.0% | |
| CTCATTTAGTACGACCACTC+GGG | + | chr8_1:70925322-70925341 | None:intergenic | 45.0% | |
| GTACATTTCTGCCTGCAGTT+CGG | - | chr8_1:70925444-70925463 | Msa1211330:CDS | 45.0% | |
| CTTGTGTTGTGCCACCCTTA+AGG | + | chr8_1:70924387-70924406 | None:intergenic | 50.0% | |
| TCCTGTGCATGGATGTTGTG+AGG | - | chr8_1:70924618-70924637 | Msa1211330:intron | 50.0% | |
| TCCTCACAACATCCATGCAC+AGG | + | chr8_1:70924622-70924641 | None:intergenic | 50.0% | |
| ! | AAAGAGTGCGAGCGCATTGA+AGG | + | chr8_1:70924797-70924816 | None:intergenic | 50.0% |
| !! | GGGCACTGAAGCCACAAATT+AGG | + | chr8_1:70924970-70924989 | None:intergenic | 50.0% |
| GACAAGATTACCTCTGGTCG+AGG | + | chr8_1:70925274-70925293 | None:intergenic | 50.0% | |
| CGACCAGAGGTAATCTTGTC+AGG | - | chr8_1:70925274-70925293 | Msa1211330:CDS | 50.0% | |
| ACCCCTGACAAGATTACCTC+TGG | + | chr8_1:70925280-70925299 | None:intergenic | 50.0% | |
| CTTGTGTTGTGCCACCCTTA+AGG | + | chr8_1:70924387-70924406 | None:intergenic | 50.0% | |
| TCCTGTGCATGGATGTTGTG+AGG | - | chr8_1:70924618-70924637 | Msa1211330:intron | 50.0% | |
| TCCTCACAACATCCATGCAC+AGG | + | chr8_1:70924622-70924641 | None:intergenic | 50.0% | |
| ! | AAAGAGTGCGAGCGCATTGA+AGG | + | chr8_1:70924797-70924816 | None:intergenic | 50.0% |
| !! | GGGCACTGAAGCCACAAATT+AGG | + | chr8_1:70924970-70924989 | None:intergenic | 50.0% |
| GACAAGATTACCTCTGGTCG+AGG | + | chr8_1:70925274-70925293 | None:intergenic | 50.0% | |
| CGACCAGAGGTAATCTTGTC+AGG | - | chr8_1:70925274-70925293 | Msa1211330:CDS | 50.0% | |
| ACCCCTGACAAGATTACCTC+TGG | + | chr8_1:70925280-70925299 | None:intergenic | 50.0% | |
| !! | TGATAGTGGCTGCGACTCTG+AGG | + | chr8_1:70925046-70925065 | None:intergenic | 55.0% |
| CCACTCGGGGAGGTTAGAAT+TGG | + | chr8_1:70925308-70925327 | None:intergenic | 55.0% | |
| CCAATTCTAACCTCCCCGAG+TGG | - | chr8_1:70925305-70925324 | Msa1211330:CDS | 55.0% | |
| TTTAGTACGACCACTCGGGG+AGG | + | chr8_1:70925318-70925337 | None:intergenic | 55.0% | |
| CAGCGATTCTTCCGAACTGC+AGG | + | chr8_1:70925458-70925477 | None:intergenic | 55.0% | |
| !! | TGATAGTGGCTGCGACTCTG+AGG | + | chr8_1:70925046-70925065 | None:intergenic | 55.0% |
| CCACTCGGGGAGGTTAGAAT+TGG | + | chr8_1:70925308-70925327 | None:intergenic | 55.0% | |
| CCAATTCTAACCTCCCCGAG+TGG | - | chr8_1:70925305-70925324 | Msa1211330:CDS | 55.0% | |
| TTTAGTACGACCACTCGGGG+AGG | + | chr8_1:70925318-70925337 | None:intergenic | 55.0% | |
| CAGCGATTCTTCCGAACTGC+AGG | + | chr8_1:70925458-70925477 | None:intergenic | 55.0% | |
| CCTCTGGTCGAGGGAACCTG+TGG | + | chr8_1:70925264-70925283 | None:intergenic | 65.0% | |
| CCACAGGTTCCCTCGACCAG+AGG | - | chr8_1:70925261-70925280 | Msa1211330:CDS | 65.0% | |
| CCTCTGGTCGAGGGAACCTG+TGG | + | chr8_1:70925264-70925283 | None:intergenic | 65.0% | |
| CCACAGGTTCCCTCGACCAG+AGG | - | chr8_1:70925261-70925280 | Msa1211330:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 70924379 | 70925564 | 70924379 | ID=Msa1211330;Name=Msa1211330 |
| chr8_1 | mRNA | 70924379 | 70925564 | 70924379 | ID=Msa1211330-mRNA-1;Parent=Msa1211330;Name=Msa1211330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|0|0|1|1|1|2|0|299 |
| chr8_1 | exon | 70924768 | 70925564 | 70924768 | ID=Msa1211330-mRNA-1:exon:23776;Parent=Msa1211330-mRNA-1 |
| chr8_1 | exon | 70924379 | 70924481 | 70924379 | ID=Msa1211330-mRNA-1:exon:23775;Parent=Msa1211330-mRNA-1 |
| chr8_1 | CDS | 70924768 | 70925564 | 70924768 | ID=Msa1211330-mRNA-1:cds;Parent=Msa1211330-mRNA-1 |
| chr8_1 | CDS | 70924379 | 70924481 | 70924379 | ID=Msa1211330-mRNA-1:cds;Parent=Msa1211330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
