AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1211430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1211430 MtrunA17_Chr4g0060031 94.065 337 16 2 1 333 1 337 0.0 588
Msa1211430 MtrunA17_Chr5g0415941 46.356 343 153 10 1 331 1 324 2.15e-82 253
Msa1211430 MtrunA17_Chr8g0362751 41.718 326 126 11 19 333 14 286 7.60e-55 181
Msa1211430 MtrunA17_Chr6g0450991 44.355 248 102 7 31 275 14 228 5.93e-47 160
Msa1211430 MtrunA17_Chr8g0362151 45.550 191 70 6 28 199 11 186 8.74e-39 139
Msa1211430 MtrunA17_Chr5g0416391 53.846 143 56 3 66 199 45 186 5.25e-38 137
Msa1211430 MtrunA17_Chr6g0453021 45.833 168 75 4 48 204 50 212 4.23e-37 134
Msa1211430 MtrunA17_Chr3g0123691 58.559 111 46 0 86 196 51 161 2.50e-36 132
Msa1211430 MtrunA17_Chr3g0119291 62.857 105 39 0 94 198 58 162 1.76e-35 129
Msa1211430 MtrunA17_Chr8g0381741 60.784 102 40 0 94 195 53 154 5.34e-35 129
Msa1211430 MtrunA17_Chr7g0217111 57.143 112 46 2 81 190 64 175 9.03e-32 120
Msa1211430 MtrunA17_Chr7g0265521 48.148 135 50 4 94 228 62 176 8.02e-30 114
Msa1211430 MtrunA17_Chr1g0179581 57.426 101 35 2 84 184 18 110 1.27e-28 107
Msa1211430 MtrunA17_Chr3g0127511 55.789 95 42 0 97 191 40 134 2.92e-28 110
Msa1211430 MtrunA17_Chr8g0346251 47.778 90 47 0 102 191 48 137 3.60e-24 99.0
Msa1211430 MtrunA17_Chr5g0403701 45.872 109 52 1 95 203 53 154 1.12e-21 91.3
Msa1211430 MtrunA17_Chr5g0403691 49.091 110 54 2 95 203 53 161 2.34e-21 90.9
Msa1211430 MtrunA17_Chr4g0055851 50.926 108 44 2 80 187 107 205 8.65e-21 90.5
Msa1211430 MtrunA17_Chr5g0399491 43.448 145 61 4 45 187 96 221 1.52e-19 87.4
Msa1211430 MtrunA17_Chr1g0175651 46.341 123 55 3 69 187 124 239 1.25e-18 84.7
Msa1211430 MtrunA17_Chr7g0258831 37.931 174 93 5 11 182 87 247 3.82e-18 83.6
Msa1211430 MtrunA17_Chr2g0308611 50.000 90 45 0 93 182 198 287 4.01e-18 84.0
Msa1211430 MtrunA17_Chr8g0335131 51.613 93 38 2 95 187 146 231 1.74e-17 81.6
Msa1211430 MtrunA17_Chr4g0054421 46.154 104 49 1 84 187 116 212 2.17e-17 80.9
Msa1211430 MtrunA17_Chr1g0151971 36.905 168 78 4 26 182 67 217 1.36e-16 79.0
Msa1211430 MtrunA17_Chr5g0400781 44.792 96 53 0 87 182 115 210 6.26e-16 76.6
Msa1211430 MtrunA17_Chr4g0044091 45.161 93 44 1 95 187 65 150 2.04e-14 70.9
Msa1211430 MtrunA17_Chr3g0135331 48.611 72 37 0 95 166 145 216 2.06e-14 72.8
Msa1211430 MtrunA17_Chr4g0071411 44.444 90 43 1 98 187 91 173 5.39e-14 70.1
Msa1211430 MtrunA17_Chr2g0299031 40.909 88 52 0 95 182 82 169 1.67e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1211430 AT3G01470.1 46.119 219 86 6 52 267 32 221 6.03e-46 157
Msa1211430 AT4G40060.1 52.564 156 70 2 44 195 4 159 1.29e-39 141
Msa1211430 AT2G22430.1 63.636 99 36 0 93 191 60 158 3.50e-36 133
Msa1211430 AT3G01220.1 59.130 115 43 1 94 208 86 196 3.72e-36 132
Msa1211430 AT5G15150.1 65.957 94 32 0 94 187 114 207 5.73e-36 132
Msa1211430 AT1G69780.1 47.590 166 62 4 44 199 44 194 5.81e-36 132
Msa1211430 AT5G65310.2 62.626 99 37 0 93 191 52 150 2.65e-35 130
Msa1211430 AT5G65310.1 62.626 99 37 0 93 191 70 168 3.07e-35 130
Msa1211430 AT1G27045.1 44.134 179 80 4 23 200 14 173 4.67e-33 122
Msa1211430 AT1G26960.1 48.322 149 64 4 43 190 30 166 1.68e-31 119
Msa1211430 AT1G27045.4 45.181 166 71 4 23 187 14 160 3.80e-31 118
Msa1211430 AT1G27045.5 53.846 117 53 1 83 198 12 128 3.91e-30 113
Msa1211430 AT1G27045.3 53.846 117 53 1 83 198 12 128 3.91e-30 113
Msa1211430 AT1G27045.2 53.846 117 53 1 83 198 4 120 4.09e-30 113
Msa1211430 AT2G18550.1 48.120 133 54 3 65 197 39 156 4.32e-28 109
Msa1211430 AT2G46680.1 48.148 108 56 0 91 198 28 135 1.72e-27 108
Msa1211430 AT3G61890.1 51.485 101 48 1 98 198 33 132 7.45e-27 106
Msa1211430 AT2G46680.2 52.688 93 44 0 91 183 28 120 4.93e-26 104
Msa1211430 AT4G36740.2 50.485 103 44 1 95 197 56 151 2.16e-25 102
Msa1211430 AT4G36740.1 50.485 103 44 1 95 197 55 150 2.17e-25 102
Msa1211430 AT2G36610.1 38.764 178 79 7 45 203 12 178 2.40e-25 100
Msa1211430 AT5G03790.1 45.528 123 54 3 95 211 77 192 3.04e-25 102
Msa1211430 AT5G03790.2 53.763 93 36 1 95 187 77 162 4.66e-25 100
Msa1211430 AT5G66700.1 48.454 97 43 1 95 191 71 160 1.85e-22 94.4
Msa1211430 AT5G06710.1 48.624 109 40 2 79 187 183 275 1.56e-18 85.5
Msa1211430 AT3G60390.1 46.281 121 54 3 67 187 138 247 1.77e-18 85.1
Msa1211430 AT5G47370.1 48.077 104 47 1 84 187 119 215 7.67e-18 82.8
Msa1211430 AT5G53980.1 46.667 90 48 0 95 184 11 100 7.85e-18 79.7
Msa1211430 AT2G44910.1 51.613 93 38 1 95 187 163 248 1.34e-17 82.4
Msa1211430 AT4G16780.1 45.133 113 47 2 75 187 117 214 1.77e-17 81.6
Msa1211430 AT4G17460.1 47.619 105 47 2 84 187 123 220 3.16e-17 80.9
Msa1211430 AT2G22800.1 43.750 96 54 0 87 182 105 200 1.96e-15 75.9
Msa1211430 AT2G01430.1 42.478 113 51 3 80 187 121 224 7.57e-15 73.9
Msa1211430 AT4G37790.1 43.810 105 52 2 83 187 114 211 2.03e-14 72.8
Msa1211430 AT5G06710.4 54.412 68 22 1 79 146 183 241 1.68e-13 70.1
Msa1211430 AT2G01430.2 46.575 73 32 2 80 147 121 191 6.89e-12 64.3
Msa1211430 AT1G70920.1 43.011 93 46 1 95 187 69 154 2.01e-11 63.2
Msa1211430 AT1G70920.2 43.011 93 46 1 95 187 39 124 2.42e-11 62.0

Find 107 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAAGAATTAATGCTTTC+TGG 0.113684 8_1:-71105325 Msa1211430:three_prime_UTR
TCTTCTTCTGCTTCTTTCTT+TGG 0.167637 8_1:-71107267 Msa1211430:intron
TAATTTACAATGCAACAAAA+TGG 0.203752 8_1:+71105375 None:intergenic
CTCACTCACTTTGCACAAAA+AGG 0.217554 8_1:+71105516 None:intergenic
AGCCTCAACAATTGACAATT+TGG 0.241624 8_1:+71105899 None:intergenic
TTGTAAATTAATGAAACTTT+TGG 0.268912 8_1:-71105362 Msa1211430:three_prime_UTR
TTTAATAGGTTCAAGATCTA+TGG 0.283771 8_1:-71106812 Msa1211430:intron
TGAGAGACGCCGTTTCTTCT+CGG 0.306253 8_1:+71106681 None:intergenic
ATTTCATTTCATACCCGAAA+TGG 0.312835 8_1:-71105437 Msa1211430:three_prime_UTR
TTCATCAAGGAGTGACATAT+TGG 0.318103 8_1:-71105844 Msa1211430:CDS
TACAAGAGCGACACCCATTT+CGG 0.319905 8_1:+71105423 None:intergenic
TTGTAGCTATATAGCTATTA+TGG 0.322660 8_1:-71107486 Msa1211430:five_prime_UTR
TTTATATTTGAAATATTTGC+AGG 0.339240 8_1:+71107369 None:intergenic
TCAACCAAAGGTTTATTCAT+TGG 0.340677 8_1:+71105945 None:intergenic
ACAAGAGCGACACCCATTTC+GGG 0.341449 8_1:+71105424 None:intergenic
AGCCACGGCAAGTTGCTATT+TGG 0.343826 8_1:-71106564 Msa1211430:CDS
AAGATAATCATCAAGCTCTT+TGG 0.354671 8_1:-71105626 Msa1211430:CDS
TGTCTTCTTCATCTTGTGAT+AGG 0.364063 8_1:+71105739 None:intergenic
CCACATAATTCATCAAGTTA+TGG 0.374938 8_1:-71105660 Msa1211430:CDS
ACCAAGGTCTTTAGCTAGCT+TGG 0.382164 8_1:+71106591 None:intergenic
ATCATCAAGCTCTTTGGCCT+TGG 0.382441 8_1:-71105620 Msa1211430:CDS
AATTTACAATGCAACAAAAT+GGG 0.386090 8_1:+71105376 None:intergenic
GAAATGGGTGTCGCTCTTGT+AGG 0.399701 8_1:-71105421 Msa1211430:three_prime_UTR
TATAAGATAAAGTTTAACTT+TGG 0.405131 8_1:-71107453 Msa1211430:five_prime_UTR
GGATTTGATCTTGACGAAAA+CGG 0.405214 8_1:-71106736 Msa1211430:CDS
ATAATCCTTCTCAAGCTGTT+TGG 0.413395 8_1:+71106513 None:intergenic
TGAAACAAAGGAATTCTTGC+AGG 0.414573 8_1:-71105973 Msa1211430:CDS
GATTAAAGATCTAAGACTTA+TGG 0.420684 8_1:+71107526 None:intergenic
GGAGGTGTGGGAACAACGGC+AGG 0.421932 8_1:+71107590 None:intergenic
TTGTGATAGATAAATATATA+TGG 0.425813 8_1:-71107418 Msa1211430:exon
AAGGAAGGAAAAGGTGCAAC+AGG 0.438515 8_1:-71106771 Msa1211430:CDS
TGATTTCAGTGATGTGTGAT+TGG 0.442504 8_1:+71107568 None:intergenic
ACCACCAATGAATAAACCTT+TGG 0.443957 8_1:-71105949 Msa1211430:CDS
ACCTTCTCAAAGAGAAAGAT+AGG 0.463134 8_1:-71106444 Msa1211430:CDS
AGAAAGAAGCAGAAGAAGAA+AGG 0.470780 8_1:+71107271 None:intergenic
TTTCATTTCATACCCGAAAT+GGG 0.470838 8_1:-71105436 Msa1211430:three_prime_UTR
CGAAAACGGCGAGGATGAGA+TGG 0.475286 8_1:-71106722 Msa1211430:CDS
TATATAGCTACAAGCTAGCT+AGG 0.478244 8_1:+71107496 None:intergenic
AGACTTATGGCGGAAGAAGC+TGG 0.487077 8_1:+71107539 None:intergenic
AGGTGCAACAGGTCTTTCTT+CGG 0.489691 8_1:-71106760 Msa1211430:CDS
ACCAAAGGTTTATTCATTGG+TGG 0.490303 8_1:+71105948 None:intergenic
TTTGGTTGATTCAGTTTCTG+AGG 0.492605 8_1:-71105931 Msa1211430:CDS
GGTAGCAAGCCTAACTGAAA+AGG 0.493946 8_1:-71106042 Msa1211430:CDS
ACCTATCTTTCTCTTTGAGA+AGG 0.495244 8_1:+71106443 None:intergenic
AAGACCTTGGTTTGCAGCCA+CGG 0.500948 8_1:-71106579 Msa1211430:CDS
AAAGAGAATCAAACAAGAGA+TGG 0.506055 8_1:+71107687 None:intergenic
ATCCAAATTGTCAATTGTTG+AGG 0.508800 8_1:-71105901 Msa1211430:CDS
TCGCTCTTGTAGGATAGAAA+TGG 0.509467 8_1:-71105411 Msa1211430:three_prime_UTR
TTCATCAATCCGAGAAGAAA+CGG 0.510950 8_1:-71106690 Msa1211430:CDS
GACTCAATACAAAGTTTCAA+AGG 0.516349 8_1:+71107653 None:intergenic
TTGGTTGATTCAGTTTCTGA+GGG 0.528561 8_1:-71105930 Msa1211430:CDS
GATTTCAGTGATGTGTGATT+GGG 0.528729 8_1:+71107569 None:intergenic
TGATGTGTGATTGGGAGGTG+TGG 0.530656 8_1:+71107577 None:intergenic
TCTTGAGAAAAGCTTTGAAG+AGG 0.530902 8_1:-71106641 Msa1211430:CDS
TTCAGTGATGTGTGATTGGG+AGG 0.530989 8_1:+71107572 None:intergenic
CCATAACTTGATGAATTATG+TGG 0.532883 8_1:+71105660 None:intergenic
ACTCAATACAAAGTTTCAAA+GGG 0.532890 8_1:+71107654 None:intergenic
GAAAGATAGGTTACAATCTG+AGG 0.533848 8_1:-71106431 Msa1211430:intron
AAGATGTTCAAGGAAGGAAA+AGG 0.536023 8_1:-71106780 Msa1211430:CDS
ATACAAAGTTTCAAAGGGAG+GGG 0.536751 8_1:+71107659 None:intergenic
TTCAAAACCGTCGTGCAAGA+TGG 0.537462 8_1:-71106540 Msa1211430:CDS
CCATTACTCCGATCAAAAGA+AGG 0.543323 8_1:+71107619 None:intergenic
ATTTGAACATGAATATCAAT+CGG 0.545738 8_1:-71105763 Msa1211430:CDS
ACCAAGCTAGCTAAAGACCT+TGG 0.547629 8_1:-71106592 Msa1211430:CDS
GATTCAGTTTCTGAGGGTGA+AGG 0.550989 8_1:-71105924 Msa1211430:CDS
ATTTACAATGCAACAAAATG+GGG 0.551950 8_1:+71105377 None:intergenic
GAAAGGAAAAGAGAATCAAG+AGG 0.552633 8_1:+71107288 None:intergenic
AATACAAAGTTTCAAAGGGA+GGG 0.558496 8_1:+71107658 None:intergenic
GATAGATAAATATATATGGC+GGG 0.563209 8_1:-71107414 Msa1211430:exon
ATATTCATGTTCAAATACAT+AGG 0.565080 8_1:+71105770 None:intergenic
TGCAACAGGTCTTTCTTCGG+AGG 0.566461 8_1:-71106757 Msa1211430:CDS
AGTACTCCGTCAGTGTAGCG+TGG 0.572346 8_1:+71105810 None:intergenic
ATGAAGATATTAGTTCATCA+AGG 0.573603 8_1:-71105857 Msa1211430:CDS
TGATAGATAAATATATATGG+CGG 0.578645 8_1:-71107415 Msa1211430:exon
GATAAATATATATGGCGGGT+GGG 0.581991 8_1:-71107410 Msa1211430:exon
AGATAAATATATATGGCGGG+TGG 0.584083 8_1:-71107411 Msa1211430:exon
AACTTGATGAATTATGTGGC+GGG 0.586622 8_1:+71105664 None:intergenic
AAGTCAGAGCAATGGCAAGT+TGG 0.588019 8_1:-71105464 Msa1211430:three_prime_UTR
CTTGCCGTGGCTGCAAACCA+AGG 0.588928 8_1:+71106575 None:intergenic
TACAAAGTTTCAAAGGGAGG+GGG 0.589956 8_1:+71107660 None:intergenic
GAAGAAACGGCGTCTCTCAG+TGG 0.590083 8_1:-71106677 Msa1211430:CDS
ATCAAGTTATGGATTTCAAG+AGG 0.600284 8_1:-71105649 Msa1211430:CDS
ACAAGGTGAAAGTGAAACAA+AGG 0.601903 8_1:-71105985 Msa1211430:CDS
GATGTGTGATTGGGAGGTGT+GGG 0.603238 8_1:+71107578 None:intergenic
GTACTCCGTCAGTGTAGCGT+GGG 0.604456 8_1:+71105811 None:intergenic
ACTGACGGAGTACTAGAAAG+AGG 0.606929 8_1:-71105801 Msa1211430:CDS
TGATGAATTATGTGGCGGGT+CGG 0.611170 8_1:+71105668 None:intergenic
TGGTCTTCCATCTTGCACGA+CGG 0.616539 8_1:+71106533 None:intergenic
GAAGACCAAACAGCTTGAGA+AGG 0.620125 8_1:-71106518 Msa1211430:CDS
TAAAGATCTAAGACTTATGG+CGG 0.625239 8_1:+71107529 None:intergenic
TAACTTGATGAATTATGTGG+CGG 0.625613 8_1:+71105663 None:intergenic
CAAGAGGGAAAATTCAAACA+AGG 0.630062 8_1:-71106002 Msa1211430:CDS
CAATACAAAGTTTCAAAGGG+AGG 0.631773 8_1:+71107657 None:intergenic
CTCTTGTAGGATAGAAATGG+CGG 0.632443 8_1:-71105408 Msa1211430:three_prime_UTR
AACCAAATAGCAACTTGCCG+TGG 0.641928 8_1:+71106562 None:intergenic
AGATACAGAGACAGAACTAG+TGG 0.648443 8_1:+71107728 None:intergenic
GCGTAATAAAGTCAGAGCAA+TGG 0.653424 8_1:-71105472 Msa1211430:three_prime_UTR
CATATCATTTAATAAGACCA+AGG 0.657999 8_1:+71105603 None:intergenic
GAGGTGTGGGAACAACGGCA+GGG 0.660019 8_1:+71107591 None:intergenic
AATCAACAACAAACTCCTCG+TGG 0.662080 8_1:-71107327 Msa1211430:CDS
ATTGGGAGGTGTGGGAACAA+CGG 0.662181 8_1:+71107586 None:intergenic
ACTTGCAAGAGAGAAACAAG+AGG 0.662555 8_1:-71106018 Msa1211430:CDS
TCAGAAACTGAATCAACCAA+AGG 0.671154 8_1:+71105933 None:intergenic
TGATCTTGACGAAAACGGCG+AGG 0.684795 8_1:-71106731 Msa1211430:CDS
CTTGCAAGAGAGAAACAAGA+GGG 0.688671 8_1:-71106017 Msa1211430:CDS
ACAACAAGCTTGAACCAGAG+AGG 0.699556 8_1:-71106618 Msa1211430:CDS
TGTTGAGAAGAGTTTCCACG+AGG 0.738171 8_1:+71107312 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATATATATATTTTAAT+AGG - chr8_1:71106291-71106310 Msa1211430:intron 0.0%
!!! TATTAATTTTTACAATATCA+AGG + chr8_1:71105949-71105968 None:intergenic 10.0%
!! AAATTAATATAGTATCTTAT+AGG - chr8_1:71105960-71105979 Msa1211430:CDS 10.0%
!!! ATGTAATTTTAATAAAATAG+TGG - chr8_1:71106265-71106284 Msa1211430:intron 10.0%
!!! TTTTTTTTCTATGAGAAAAA+TGG + chr8_1:71105342-71105361 None:intergenic 15.0%
!!! TATAAGATAAAGTTTAACTT+TGG - chr8_1:71105664-71105683 Msa1211430:CDS 15.0%
!! TTGTGATAGATAAATATATA+TGG - chr8_1:71105699-71105718 Msa1211430:CDS 15.0%
!!! TTTATATTTGAAATATTTGC+AGG + chr8_1:71105751-71105770 None:intergenic 15.0%
!!! GTTTCAAGATTTTAAATAAA+TGG - chr8_1:71106064-71106083 Msa1211430:intron 15.0%
!!! TTTTTTTTTTGAGAAAGTAA+CGG + chr8_1:71106123-71106142 None:intergenic 15.0%
!!! TTGTAAATTAATGAAACTTT+TGG - chr8_1:71107755-71107774 Msa1211430:five_prime_UTR 15.0%
!! TGATAGATAAATATATATGG+CGG - chr8_1:71105702-71105721 Msa1211430:CDS 20.0%
!! AAAAAAATTACCGTTACATA+TGG - chr8_1:71106142-71106161 Msa1211430:intron 20.0%
!!! CTTAATTGTAAATTTGAGAT+CGG - chr8_1:71106791-71106810 Msa1211430:CDS 20.0%
!! ACATAAAATTCATGAAATTG+TGG - chr8_1:71106986-71107005 Msa1211430:intron 20.0%
!! ATATTCATGTTCAAATACAT+AGG + chr8_1:71107350-71107369 None:intergenic 20.0%
!! ATTTGAACATGAATATCAAT+CGG - chr8_1:71107354-71107373 Msa1211430:CDS 20.0%
!! AATTTACAATGCAACAAAAT+GGG + chr8_1:71107744-71107763 None:intergenic 20.0%
!! TAATTTACAATGCAACAAAA+TGG + chr8_1:71107745-71107764 None:intergenic 20.0%
!! TTAAAAGAATTAATGCTTTC+TGG - chr8_1:71107792-71107811 Msa1211430:five_prime_UTR 20.0%
! ACTCAATACAAAGTTTCAAA+GGG + chr8_1:71105466-71105485 None:intergenic 25.0%
!! GATTAAAGATCTAAGACTTA+TGG + chr8_1:71105594-71105613 None:intergenic 25.0%
! TTGTAGCTATATAGCTATTA+TGG - chr8_1:71105631-71105650 Msa1211430:CDS 25.0%
! GATAGATAAATATATATGGC+GGG - chr8_1:71105703-71105722 Msa1211430:CDS 25.0%
!!! ATCAATGTTTTGATGTCTTT+AGG + chr8_1:71105996-71106015 None:intergenic 25.0%
! ACTAATATGACCATATGTAA+CGG + chr8_1:71106155-71106174 None:intergenic 25.0%
! TTTAATAGGTTCAAGATCTA+TGG - chr8_1:71106305-71106324 Msa1211430:intron 25.0%
! ATGAAGATATTAGTTCATCA+AGG - chr8_1:71107260-71107279 Msa1211430:intron 25.0%
!!! TCAAGTTTTGTAAAGATGTA+AGG + chr8_1:71107415-71107434 None:intergenic 25.0%
! CATATCATTTAATAAGACCA+AGG + chr8_1:71107517-71107536 None:intergenic 25.0%
! ATTTACAATGCAACAAAATG+GGG + chr8_1:71107743-71107762 None:intergenic 25.0%
AAAGAGAATCAAACAAGAGA+TGG + chr8_1:71105433-71105452 None:intergenic 30.0%
AATACAAAGTTTCAAAGGGA+GGG + chr8_1:71105462-71105481 None:intergenic 30.0%
GACTCAATACAAAGTTTCAA+AGG + chr8_1:71105467-71105486 None:intergenic 30.0%
! TAAAGATCTAAGACTTATGG+CGG + chr8_1:71105591-71105610 None:intergenic 30.0%
!! TAGTATCTTATAGGTTGATG+CGG - chr8_1:71105969-71105988 Msa1211430:CDS 30.0%
! GTTTTTGATATCTCTGCAAA+CGG + chr8_1:71106183-71106202 None:intergenic 30.0%
TGTGGTTCACATAAAACTAT+TGG - chr8_1:71107004-71107023 Msa1211430:intron 30.0%
TCTTTGTGATGTTTCAATAC+AGG - chr8_1:71107054-71107073 Msa1211430:intron 30.0%
TCAACCAAAGGTTTATTCAT+TGG + chr8_1:71107175-71107194 None:intergenic 30.0%
ATCCAAATTGTCAATTGTTG+AGG - chr8_1:71107216-71107235 Msa1211430:intron 30.0%
!! AGTTTTGTAAAGATGTAAGG+AGG + chr8_1:71107412-71107431 None:intergenic 30.0%
TAACTTGATGAATTATGTGG+CGG + chr8_1:71107457-71107476 None:intergenic 30.0%
CCATAACTTGATGAATTATG+TGG + chr8_1:71107460-71107479 None:intergenic 30.0%
CCACATAATTCATCAAGTTA+TGG - chr8_1:71107457-71107476 Msa1211430:five_prime_UTR 30.0%
ATCAAGTTATGGATTTCAAG+AGG - chr8_1:71107468-71107487 Msa1211430:five_prime_UTR 30.0%
AAGATAATCATCAAGCTCTT+TGG - chr8_1:71107491-71107510 Msa1211430:five_prime_UTR 30.0%
ATTTCATTTCATACCCGAAA+TGG - chr8_1:71107680-71107699 Msa1211430:five_prime_UTR 30.0%
TTTCATTTCATACCCGAAAT+GGG - chr8_1:71107681-71107700 Msa1211430:five_prime_UTR 30.0%
! TGAGAAAAATGGACTCTGTT+GGG + chr8_1:71105331-71105350 None:intergenic 35.0%
! ATGAGAAAAATGGACTCTGT+TGG + chr8_1:71105332-71105351 None:intergenic 35.0%
ATACAAAGTTTCAAAGGGAG+GGG + chr8_1:71105461-71105480 None:intergenic 35.0%
CAATACAAAGTTTCAAAGGG+AGG + chr8_1:71105463-71105482 None:intergenic 35.0%
! TCTTGTTGCCTTCTTTTGAT+CGG - chr8_1:71105490-71105509 Msa1211430:three_prime_UTR 35.0%
! CTTCTTTTGATCGGAGTAAT+GGG - chr8_1:71105499-71105518 Msa1211430:three_prime_UTR 35.0%
GATTTCAGTGATGTGTGATT+GGG + chr8_1:71105551-71105570 None:intergenic 35.0%
TGATTTCAGTGATGTGTGAT+TGG + chr8_1:71105552-71105571 None:intergenic 35.0%
TATATAGCTACAAGCTAGCT+AGG + chr8_1:71105624-71105643 None:intergenic 35.0%
! AGATAAATATATATGGCGGG+TGG - chr8_1:71105706-71105725 Msa1211430:CDS 35.0%
!! GATAAATATATATGGCGGGT+GGG - chr8_1:71105707-71105726 Msa1211430:CDS 35.0%
GAAAGGAAAAGAGAATCAAG+AGG + chr8_1:71105832-71105851 None:intergenic 35.0%
AGAAAGAAGCAGAAGAAGAA+AGG + chr8_1:71105849-71105868 None:intergenic 35.0%
TCTTCTTCTGCTTCTTTCTT+TGG - chr8_1:71105850-71105869 Msa1211430:CDS 35.0%
!!! GTGAGTTTTGAAGATGTTCA+AGG - chr8_1:71106327-71106346 Msa1211430:intron 35.0%
!!! GTTTTGAAGATGTTCAAGGA+AGG - chr8_1:71106331-71106350 Msa1211430:intron 35.0%
AAGATGTTCAAGGAAGGAAA+AGG - chr8_1:71106337-71106356 Msa1211430:intron 35.0%
GGATTTGATCTTGACGAAAA+CGG - chr8_1:71106381-71106400 Msa1211430:intron 35.0%
TTCATCAATCCGAGAAGAAA+CGG - chr8_1:71106427-71106446 Msa1211430:intron 35.0%
!! TCTTGAGAAAAGCTTTGAAG+AGG - chr8_1:71106476-71106495 Msa1211430:CDS 35.0%
ATAATCCTTCTCAAGCTGTT+TGG + chr8_1:71106607-71106626 None:intergenic 35.0%
ACCTTCTCAAAGAGAAAGAT+AGG - chr8_1:71106673-71106692 Msa1211430:CDS 35.0%
! ACCTATCTTTCTCTTTGAGA+AGG + chr8_1:71106677-71106696 None:intergenic 35.0%
GAAAGATAGGTTACAATCTG+AGG - chr8_1:71106686-71106705 Msa1211430:CDS 35.0%
! TTGCAAGTACCTTTTCAGTT+AGG + chr8_1:71107087-71107106 None:intergenic 35.0%
CAAGAGGGAAAATTCAAACA+AGG - chr8_1:71107115-71107134 Msa1211430:intron 35.0%
ACAAGGTGAAAGTGAAACAA+AGG - chr8_1:71107132-71107151 Msa1211430:intron 35.0%
TGAAACAAAGGAATTCTTGC+AGG - chr8_1:71107144-71107163 Msa1211430:intron 35.0%
ACCACCAATGAATAAACCTT+TGG - chr8_1:71107168-71107187 Msa1211430:intron 35.0%
ACCAAAGGTTTATTCATTGG+TGG + chr8_1:71107172-71107191 None:intergenic 35.0%
TCAGAAACTGAATCAACCAA+AGG + chr8_1:71107187-71107206 None:intergenic 35.0%
! TTTGGTTGATTCAGTTTCTG+AGG - chr8_1:71107186-71107205 Msa1211430:intron 35.0%
! TTGGTTGATTCAGTTTCTGA+GGG - chr8_1:71107187-71107206 Msa1211430:intron 35.0%
AGCCTCAACAATTGACAATT+TGG + chr8_1:71107221-71107240 None:intergenic 35.0%
TTCATCAAGGAGTGACATAT+TGG - chr8_1:71107273-71107292 Msa1211430:CDS 35.0%
TGTCTTCTTCATCTTGTGAT+AGG + chr8_1:71107381-71107400 None:intergenic 35.0%
AACTTGATGAATTATGTGGC+GGG + chr8_1:71107456-71107475 None:intergenic 35.0%
TCACTCACTTTGCACAAAAA+GGG + chr8_1:71107603-71107622 None:intergenic 35.0%
AGATACAGAGACAGAACTAG+TGG + chr8_1:71105392-71105411 None:intergenic 40.0%
TACAAAGTTTCAAAGGGAGG+GGG + chr8_1:71105460-71105479 None:intergenic 40.0%
CCATTACTCCGATCAAAAGA+AGG + chr8_1:71105501-71105520 None:intergenic 40.0%
! CCTTCTTTTGATCGGAGTAA+TGG - chr8_1:71105498-71105517 Msa1211430:three_prime_UTR 40.0%
!!! GGTGGGAGAGTTTTTTCAAA+TGG - chr8_1:71105724-71105743 Msa1211430:CDS 40.0%
AATCAACAACAAACTCCTCG+TGG - chr8_1:71105790-71105809 Msa1211430:CDS 40.0%
TGCGATTCAGATTGCAATCA+TGG - chr8_1:71106018-71106037 Msa1211430:CDS 40.0%
ACTTGCAAGAGAGAAACAAG+AGG - chr8_1:71107099-71107118 Msa1211430:intron 40.0%
CTTGCAAGAGAGAAACAAGA+GGG - chr8_1:71107100-71107119 Msa1211430:intron 40.0%
CTCACTCACTTTGCACAAAA+AGG + chr8_1:71107604-71107623 None:intergenic 40.0%
GCGTAATAAAGTCAGAGCAA+TGG - chr8_1:71107645-71107664 Msa1211430:five_prime_UTR 40.0%
TCGCTCTTGTAGGATAGAAA+TGG - chr8_1:71107706-71107725 Msa1211430:five_prime_UTR 40.0%
CTCTTGTAGGATAGAAATGG+CGG - chr8_1:71107709-71107728 Msa1211430:five_prime_UTR 40.0%
TTCAGTGATGTGTGATTGGG+AGG + chr8_1:71105548-71105567 None:intergenic 45.0%
TGTTGAGAAGAGTTTCCACG+AGG + chr8_1:71105808-71105827 None:intergenic 45.0%
CATAATTCAGATCGCAGTCG+CGG - chr8_1:71106211-71106230 Msa1211430:intron 45.0%
! AAGGAAGGAAAAGGTGCAAC+AGG - chr8_1:71106346-71106365 Msa1211430:intron 45.0%
AGGTGCAACAGGTCTTTCTT+CGG - chr8_1:71106357-71106376 Msa1211430:intron 45.0%
ACAACAAGCTTGAACCAGAG+AGG - chr8_1:71106499-71106518 Msa1211430:CDS 45.0%
ACCAAGCTAGCTAAAGACCT+TGG - chr8_1:71106525-71106544 Msa1211430:CDS 45.0%
ACCAAGGTCTTTAGCTAGCT+TGG + chr8_1:71106529-71106548 None:intergenic 45.0%
AACCAAATAGCAACTTGCCG+TGG + chr8_1:71106558-71106577 None:intergenic 45.0%
TTCAAAACCGTCGTGCAAGA+TGG - chr8_1:71106577-71106596 Msa1211430:CDS 45.0%
GAAGACCAAACAGCTTGAGA+AGG - chr8_1:71106599-71106618 Msa1211430:CDS 45.0%
GGTAGCAAGCCTAACTGAAA+AGG - chr8_1:71107075-71107094 Msa1211430:intron 45.0%
! GATTCAGTTTCTGAGGGTGA+AGG - chr8_1:71107193-71107212 Msa1211430:intron 45.0%
! ACTGACGGAGTACTAGAAAG+AGG - chr8_1:71107316-71107335 Msa1211430:CDS 45.0%
!! TGATGAATTATGTGGCGGGT+CGG + chr8_1:71107452-71107471 None:intergenic 45.0%
ATCATCAAGCTCTTTGGCCT+TGG - chr8_1:71107497-71107516 Msa1211430:five_prime_UTR 45.0%
AAGTCAGAGCAATGGCAAGT+TGG - chr8_1:71107653-71107672 Msa1211430:five_prime_UTR 45.0%
TACAAGAGCGACACCCATTT+CGG + chr8_1:71107697-71107716 None:intergenic 45.0%
! ATTGGGAGGTGTGGGAACAA+CGG + chr8_1:71105534-71105553 None:intergenic 50.0%
! GATGTGTGATTGGGAGGTGT+GGG + chr8_1:71105542-71105561 None:intergenic 50.0%
TGATGTGTGATTGGGAGGTG+TGG + chr8_1:71105543-71105562 None:intergenic 50.0%
! AGACTTATGGCGGAAGAAGC+TGG + chr8_1:71105581-71105600 None:intergenic 50.0%
TGCAACAGGTCTTTCTTCGG+AGG - chr8_1:71106360-71106379 Msa1211430:intron 50.0%
TGATCTTGACGAAAACGGCG+AGG - chr8_1:71106386-71106405 Msa1211430:intron 50.0%
TGAGAGACGCCGTTTCTTCT+CGG + chr8_1:71106439-71106458 None:intergenic 50.0%
!! GCTAGCTTGGTTTTCCTCTC+TGG + chr8_1:71106516-71106535 None:intergenic 50.0%
!! AAGACCTTGGTTTGCAGCCA+CGG - chr8_1:71106538-71106557 Msa1211430:CDS 50.0%
! AGCCACGGCAAGTTGCTATT+TGG - chr8_1:71106553-71106572 Msa1211430:CDS 50.0%
TGGTCTTCCATCTTGCACGA+CGG + chr8_1:71106587-71106606 None:intergenic 50.0%
ACAAGAGCGACACCCATTTC+GGG + chr8_1:71107696-71107715 None:intergenic 50.0%
!! GAAATGGGTGTCGCTCTTGT+AGG - chr8_1:71107696-71107715 Msa1211430:five_prime_UTR 50.0%
CGAAAACGGCGAGGATGAGA+TGG - chr8_1:71106395-71106414 Msa1211430:intron 55.0%
GAAGAAACGGCGTCTCTCAG+TGG - chr8_1:71106440-71106459 Msa1211430:CDS 55.0%
GTACTCCGTCAGTGTAGCGT+GGG + chr8_1:71107309-71107328 None:intergenic 55.0%
AGTACTCCGTCAGTGTAGCG+TGG + chr8_1:71107310-71107329 None:intergenic 55.0%
GAGGTGTGGGAACAACGGCA+GGG + chr8_1:71105529-71105548 None:intergenic 60.0%
CTTGCCGTGGCTGCAAACCA+AGG + chr8_1:71106545-71106564 None:intergenic 60.0%
GACAGCCCACGCTACACTGA+CGG - chr8_1:71107301-71107320 Msa1211430:CDS 60.0%
! GGAGGTGTGGGAACAACGGC+AGG + chr8_1:71105530-71105549 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 71105322 71107817 71105322 ID=Msa1211430;Name=Msa1211430
chr8_1 mRNA 71105322 71107817 71105322 ID=Msa1211430-mRNA-1;Parent=Msa1211430;Name=Msa1211430-mRNA-1;_AED=0.01;_eAED=0.01;_QI=396|1|1|1|1|1|3|289|333
chr8_1 exon 71105322 71106063 71105322 ID=Msa1211430-mRNA-1:exon:23872;Parent=Msa1211430-mRNA-1
chr8_1 exon 71106432 71106826 71106432 ID=Msa1211430-mRNA-1:exon:23871;Parent=Msa1211430-mRNA-1
chr8_1 exon 71107268 71107817 71107268 ID=Msa1211430-mRNA-1:exon:23870;Parent=Msa1211430-mRNA-1
chr8_1 five_prime_UTR 71107422 71107817 71107422 ID=Msa1211430-mRNA-1:five_prime_utr;Parent=Msa1211430-mRNA-1
chr8_1 CDS 71107268 71107421 71107268 ID=Msa1211430-mRNA-1:cds;Parent=Msa1211430-mRNA-1
chr8_1 CDS 71106432 71106826 71106432 ID=Msa1211430-mRNA-1:cds;Parent=Msa1211430-mRNA-1
chr8_1 CDS 71105611 71106063 71105611 ID=Msa1211430-mRNA-1:cds;Parent=Msa1211430-mRNA-1
chr8_1 three_prime_UTR 71105322 71105610 71105322 ID=Msa1211430-mRNA-1:three_prime_utr;Parent=Msa1211430-mRNA-1
Gene Sequence

>Msa1211430

ATGGCGGGTGGGAGAGTTTTTTCAAATGGTCCTGCAAATATTTCAAATATAAATATGAATATTTTGCTTCAGAATCAACAACAAACTCCTCGTGGAAACTCTTCTCAACAACCTCTTGATTCTCTTTTCCTTTCTTCTTCTGCTTCTTTCTTTGGTTCAAGATCTATGGTGAGTTTTGAAGATGTTCAAGGAAGGAAAAGGTGCAACAGGTCTTTCTTCGGAGGATTTGATCTTGACGAAAACGGCGAGGATGAGATGGATGAGTACTTTCATCAATCCGAGAAGAAACGGCGTCTCTCAGTGGATCAAGTTCAGTTTCTTGAGAAAAGCTTTGAAGAGGACAACAAGCTTGAACCAGAGAGGAAAACCAAGCTAGCTAAAGACCTTGGTTTGCAGCCACGGCAAGTTGCTATTTGGTTTCAAAACCGTCGTGCAAGATGGAAGACCAAACAGCTTGAGAAGGATTATGATTCTCTTAATGATAGTTATGAGTCTCTTAAGACAGATTATGACAACCTTCTCAAAGAGAAAGATAGGTTACAATCTGAGGTAGCAAGCCTAACTGAAAAGGTACTTGCAAGAGAGAAACAAGAGGGAAAATTCAAACAAGGTGAAAGTGAAACAAAGGAATTCTTGCAGGAACCACCAATGAATAAACCTTTGGTTGATTCAGTTTCTGAGGGTGAAGGATCCAAATTGTCAATTGTTGAGGCTTCTAATAACAATAGCAAACATGAAGATATTAGTTCATCAAGGAGTGACATATTGGATTGTGACAGCCCACGCTACACTGACGGAGTACTAGAAAGAGGTGATTCTTCCTATGTATTTGAACATGAATATCAATCGGACCTATCACAAGATGAAGAAGACAATTTATTGCCTCCTTACATCTTTACAAAACTTGAAGATGTGAATTACTCCGACCCGCCACATAATTCATCAAGTTATGGATTTCAAGAGGAAGATAATCATCAAGCTCTTTGGCCTTGGTCTTATTAA
Protein sequence

>Msa1211430

MAGGRVFSNGPANISNINMNILLQNQQQTPRGNSSQQPLDSLFLSSSASFFGSRSMVSFEDVQGRKRCNRSFFGGFDLDENGEDEMDEYFHQSEKKRRLSVDQVQFLEKSFEEDNKLEPERKTKLAKDLGLQPRQVAIWFQNRRARWKTKQLEKDYDSLNDSYESLKTDYDNLLKEKDRLQSEVASLTEKVLAREKQEGKFKQGESETKEFLQEPPMNKPLVDSVSEGEGSKLSIVEASNNNSKHEDISSSRSDILDCDSPRYTDGVLERGDSSYVFEHEYQSDLSQDEEDNLLPPYIFTKLEDVNYSDPPHNSSSYGFQEEDNHQALWPWSY*