AlfalfaGEDB | Msa1211970

M. sativa cultivar zhongmu-4 / Msa1211970

KEGG: K04454|K09260
GO: GO:0001558 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009555 GO:0009889 GO:0010152 GO:0010468 GO:0010556 GO:0010769 GO:0019219 GO:0019222 GO:0021700 GO:0022603 GO:0022604 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0040008 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044464 GO:0045595 GO:0048229 GO:0048638 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051239 GO:0051252 GO:0051510 GO:0060255 GO:0060284 GO:0065007 GO:0080090 GO:0080092 GO:0140110 GO:1903506 GO:2000112 GO:2000241 GO:2001141
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211970	sp\|Q7X9I0\|AGL65_ARATH	47.508	301	113	7	1	270	1	287	8.17e-80	251

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211970	A0A072USA5	90.449	356	8	1	1	330	1	356	0.0	654

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TFs/TRs:

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Gene ID	Type	Classification
Msa1211970	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211970	MtrunA17_Chr4g0060841	90.449	356	8	1	1	330	1	356	0.0	654
Msa1211970	MtrunA17_Chr3g0134451	35.211	213	106	8	1	191	1	203	2.78e-26	106
Msa1211970	MtrunA17_Chr5g0417661	70.149	67	20	0	1	67	56	122	2.95e-25	98.6
Msa1211970	MtrunA17_Chr5g0417671	81.132	53	10	0	59	111	26	78	3.94e-23	91.7
Msa1211970	MtrunA17_Chr5g0425191	62.295	61	23	0	1	61	1	61	2.33e-21	87.4
Msa1211970	MtrunA17_Chr4g0044071	63.934	61	22	0	1	61	1	61	1.30e-20	85.9
Msa1211970	MtrunA17_Chr5g0427401	39.456	147	79	4	1	143	1	141	2.57e-18	82.4
Msa1211970	MtrunA17_Chr1g0159661	39.423	104	63	0	1	104	1	104	4.35e-17	78.2
Msa1211970	MtrunA17_Chr3g0125241	62.264	53	20	0	1	53	26	78	4.23e-16	75.9
Msa1211970	MtrunA17_Chr1g0173161	58.491	53	22	0	1	53	1	53	9.15e-16	71.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211970	AT1G18750.4	47.841	301	112	8	1	270	1	287	4.15e-81	251
Msa1211970	AT1G18750.1	47.508	301	113	7	1	270	1	287	8.31e-81	251
Msa1211970	AT2G03060.3	45.614	285	105	6	1	250	1	270	4.25e-71	226
Msa1211970	AT2G03060.7	45.614	285	105	6	1	250	1	270	4.25e-71	226
Msa1211970	AT2G03060.1	45.614	285	105	6	1	250	1	270	4.25e-71	226
Msa1211970	AT2G03060.2	45.614	285	105	6	1	250	1	270	5.61e-71	226
Msa1211970	AT1G18750.3	41.916	334	116	12	1	270	1	320	3.33e-66	214
Msa1211970	AT1G69540.1	39.088	307	131	8	1	266	1	292	1.45e-57	190
Msa1211970	AT1G69540.2	41.264	269	121	5	1	234	1	267	2.45e-57	190
Msa1211970	AT1G18750.2	40.392	255	107	8	47	270	1	241	5.78e-50	170

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Find 82 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTACATGCTTACCCTA+TGG	0.497229	8_1:-71799476	None:intergenic
AACCAAAGTTTATGTCATTC+AGG	0.285801	8_1:+71798849	Msa1211970:CDS
AAGAATCTCAACCTTTGTCT+TGG	0.471192	8_1:+71798786	Msa1211970:CDS
AAGGGTAAAGTCATCCCTTC+TGG	0.392867	8_1:-71798746	None:intergenic
AATAACGTAGGCTTTCCTGT+TGG	0.623309	8_1:-71796890	None:intergenic
AATTCTACAAATGACTCTTG+TGG	0.631946	8_1:+71799426	Msa1211970:CDS
ACAAATGGAAGACTCACTCA+GGG	0.670210	8_1:+71798431	Msa1211970:CDS
ACATGTCACTACTTTGGGTC+AGG	0.387955	8_1:+71799194	Msa1211970:CDS
ACTACTTTGGGTCAGGGTGG+TGG	0.504351	8_1:+71799201	Msa1211970:CDS
ACTTATTCGAAGCGGAAAAG+TGG	0.527998	8_1:+71796800	Msa1211970:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAAAGTGTGTAAAAGTCGA+AGG	+	chr8_1:71797211-71797230	Msa1211970:intron	30.0%
ATCATGATCCAATTTCTTGA+AGG	-	chr8_1:71797589-71797608	None:intergenic	30.0%
TGTAAAGATACAAGATTTCC+TGG	+	chr8_1:71797608-71797627	Msa1211970:CDS	30.0%
GTAAAGATACAAGATTTCCT+GGG	+	chr8_1:71797609-71797628	Msa1211970:CDS	30.0%
AGAATCTTAAGCTGGTAATT+AGG	+	chr8_1:71797784-71797803	Msa1211970:intron	30.0%
CAATTGAACTCATAACACTT+TGG	-	chr8_1:71797813-71797832	None:intergenic	30.0%
ATACAAAATTGGTAAGGTAG+AGG	-	chr8_1:71797998-71798017	None:intergenic	30.0%
AAGCACATACAAAATTGGTA+AGG	-	chr8_1:71798004-71798023	None:intergenic	30.0%
TTGCAAAGCACATACAAAAT+TGG	-	chr8_1:71798009-71798028	None:intergenic	30.0%
CAATCGATTGTCTATACAAA+AGG	+	chr8_1:71798461-71798480	Msa1211970:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	71796743	71799494	71796743	ID=Msa1211970;Name=Msa1211970
chr8_1	mRNA	71796743	71799494	71796743	ID=Msa1211970-mRNA-1;Parent=Msa1211970;Name=Msa1211970-mRNA-1;_AED=0.08;_eAED=0.09;_QI=0\|0\|0\|1\|1\|1\|8\|0\|333
chr8_1	exon	71796743	71796927	71796743	ID=Msa1211970-mRNA-1:exon:24170;Parent=Msa1211970-mRNA-1
chr8_1	exon	71797292	71797351	71797292	ID=Msa1211970-mRNA-1:exon:24171;Parent=Msa1211970-mRNA-1
chr8_1	exon	71797436	71797454	71797436	ID=Msa1211970-mRNA-1:exon:24172;Parent=Msa1211970-mRNA-1
chr8_1	exon	71797573	71797637	71797573	ID=Msa1211970-mRNA-1:exon:24173;Parent=Msa1211970-mRNA-1
chr8_1	exon	71798386	71798482	71798386	ID=Msa1211970-mRNA-1:exon:24174;Parent=Msa1211970-mRNA-1
chr8_1	exon	71798611	71798658	71798611	ID=Msa1211970-mRNA-1:exon:24175;Parent=Msa1211970-mRNA-1
chr8_1	exon	71798743	71798870	71798743	ID=Msa1211970-mRNA-1:exon:24176;Parent=Msa1211970-mRNA-1
chr8_1	exon	71799095	71799494	71799095	ID=Msa1211970-mRNA-1:exon:24177;Parent=Msa1211970-mRNA-1
chr8_1	CDS	71796743	71796927	71796743	ID=Msa1211970-mRNA-1:cds;Parent=Msa1211970-mRNA-1
chr8_1	CDS	71797292	71797351	71797292	ID=Msa1211970-mRNA-1:cds;Parent=Msa1211970-mRNA-1
chr8_1	CDS	71797436	71797454	71797436	ID=Msa1211970-mRNA-1:cds;Parent=Msa1211970-mRNA-1
chr8_1	CDS	71797573	71797637	71797573	ID=Msa1211970-mRNA-1:cds;Parent=Msa1211970-mRNA-1
chr8_1	CDS	71798386	71798482	71798386	ID=Msa1211970-mRNA-1:cds;Parent=Msa1211970-mRNA-1
chr8_1	CDS	71798611	71798658	71798611	ID=Msa1211970-mRNA-1:cds;Parent=Msa1211970-mRNA-1
chr8_1	CDS	71798743	71798870	71798743	ID=Msa1211970-mRNA-1:cds;Parent=Msa1211970-mRNA-1
chr8_1	CDS	71799095	71799494	71799095	ID=Msa1211970-mRNA-1:cds;Parent=Msa1211970-mRNA-1

Gene Sequence

>Msa1211970

ATGGGGAGAGTTAAATTGAAGATAAAGAAATTGGAGAGCACAAGCAACCGACATGTGACTTATTCGAAGCGGAAAAGTGGGATCTTGAAGAAAGCAAAAGAGTTATCAATTCTATGTGATATAGATATTCTCCTTCTCATGTTTTCTCCAACAGGAAAGCCTACGTTATTACAGGGAGAACGAAGCAATATGGAGGAGATCATTGCAAAATTTGCCCAACTTAGTCCACAAGAAAGGGCTAAAAGGAAAATGGAAAGTCTTGAAGCACTGAAAAAAACCTTCAAGAAATTGGATCATGATGTAAAGATACAAGATTTCCTGGGTTCAAGTTATTGGAGTAATCTGGAGAAGATCAATAACTTGGAACATCTTAGACAAATGGAAGACTCACTCAGGGAATCAATCAATCGATTGTCTATACAAAAGGAAAATCTCGAAAAGCACCAACTAATGTCACTTGAATGCGTTAACCAGTTACCAGAAGGGATGACTTTACCCTTAATGATGTCTGGTCTACAAGAATCTCAACCTTTGTCTTGGCTTCTAAGTGGTGATAATCACCAGTTGATGTTACCTAGTGAACCAAAGTTTATGTCATTCAGTGACAATGGCAATAGAGATGTTGAGTGCTCAACCAACATTTCACTCCCAAGCTATTCTGGTTATACTGGGAACTGCAAACTGGAGGTGGGAAGCTCTCCACATGTCACTACTTTGGGTCAGGGTGGTGGTACCTTGAATGAGTTAAATGGAAATCCAAATCCTTACTTAAATGTGCAACAGTGTCAGCAGTTTGCATACCCTCCTCCACAAGATCTCGAGGAAGTGAAACATCACCAACCAATCAATATCAAATCAAATAACGTGGACTACCAAGTTAATAACAATTATGATCTGCCAAAATCACTATTTGAAAATGGACACCAGTTCTGGAATTCTACAAATGACTCTTGTGGTATTGCTATGTATAACGAGAATGGTTTCCATAGGGTAAGCATGTAG

Protein sequence

>Msa1211970

MGRVKLKIKKLESTSNRHVTYSKRKSGILKKAKELSILCDIDILLLMFSPTGKPTLLQGERSNMEEIIAKFAQLSPQERAKRKMESLEALKKTFKKLDHDVKIQDFLGSSYWSNLEKINNLEHLRQMEDSLRESINRLSIQKENLEKHQLMSLECVNQLPEGMTLPLMMSGLQESQPLSWLLSGDNHQLMLPSEPKFMSFSDNGNRDVECSTNISLPSYSGYTGNCKLEVGSSPHVTTLGQGGGTLNELNGNPNPYLNVQQCQQFAYPPPQDLEEVKHHQPINIKSNNVDYQVNNNYDLPKSLFENGHQFWNSTNDSCGIAMYNENGFHRVSM*