Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217290 | G7JJ33 | 90.055 | 181 | 18 | 0 | 1 | 181 | 1 | 181 | 4.09e-111 | 323 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1217290 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217290 | MtrunA17_Chr4g0066461 | 45.045 | 111 | 42 | 3 | 77 | 181 | 1 | 98 | 2.87e-20 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTCTCTGTTACGGTTAC+TGG | 0.183266 | 8_1:+79532695 | Msa1217290:CDS |
| AGTAACCGTAACAGAGAAAT+TGG | 0.195649 | 8_1:-79532693 | None:intergenic |
| TCAAGCAATGTGGCTTTCTT+TGG | 0.199938 | 8_1:+79532719 | Msa1217290:CDS |
| GAGAGAGATTATTAGTTTGT+AGG | 0.261530 | 8_1:+79532904 | Msa1217290:CDS |
| TGTTGGTTCCGTGTCGTAAT+AGG | 0.277898 | 8_1:+79532666 | Msa1217290:CDS |
| CAAGCAATGTGGCTTTCTTT+GGG | 0.307847 | 8_1:+79532720 | Msa1217290:CDS |
| TAACAAATCAGTTTCTGAAA+TGG | 0.363545 | 8_1:+79532257 | Msa1217290:CDS |
| GATCTTGGCGATCACTGCTT+TGG | 0.383378 | 8_1:+79532850 | Msa1217290:CDS |
| AACAAATCAGTTTCTGAAAT+GGG | 0.410347 | 8_1:+79532258 | Msa1217290:CDS |
| TTACATTGGGGTAGAGAGTT+GGG | 0.421719 | 8_1:-79532386 | None:intergenic |
| GAATCCCAATTTCTCTGTTA+CGG | 0.433893 | 8_1:+79532688 | Msa1217290:CDS |
| TAATGATGGTAATTTGATCT+TGG | 0.443216 | 8_1:+79532835 | Msa1217290:CDS |
| GAAAATGTTGGAGGAGATGA+AGG | 0.445802 | 8_1:+79532604 | Msa1217290:CDS |
| GTTACATTGGGGTAGAGAGT+TGG | 0.468914 | 8_1:-79532387 | None:intergenic |
| ATCAAAGAAACCTGTTGAAG+GGG | 0.470795 | 8_1:+79532634 | Msa1217290:CDS |
| TGAAGAAAAGAAGAAAATGT+TGG | 0.489430 | 8_1:+79532592 | Msa1217290:CDS |
| GCTAATGTTTCTGTTAATGA+TGG | 0.496957 | 8_1:+79532821 | Msa1217290:CDS |
| TTTCTTTGGGATTGAATCTG+TGG | 0.501659 | 8_1:+79532733 | Msa1217290:CDS |
| GAGTTGGGACAGTGGTAGTG+AGG | 0.504434 | 8_1:-79532371 | None:intergenic |
| ATTGAATCTGTGGCTAAACC+TGG | 0.515198 | 8_1:+79532743 | Msa1217290:CDS |
| TTCAACGTTTAGAACCTCTT+CGG | 0.524218 | 8_1:-79532310 | None:intergenic |
| AAATCAAAGAAACCTGTTGA+AGG | 0.526426 | 8_1:+79532632 | Msa1217290:CDS |
| AGAAAAGAAGAAAATGTTGG+AGG | 0.526690 | 8_1:+79532595 | Msa1217290:CDS |
| AATCAAAGAAACCTGTTGAA+GGG | 0.548949 | 8_1:+79532633 | Msa1217290:CDS |
| GTAACCGTAACAGAGAAATT+GGG | 0.551700 | 8_1:-79532692 | None:intergenic |
| TAAACGTTGAACGTGAAAGA+AGG | 0.567677 | 8_1:+79532322 | Msa1217290:CDS |
| TCAAAGAAACCTGTTGAAGG+GGG | 0.622647 | 8_1:+79532635 | Msa1217290:CDS |
| CACTACTACTAAAACCGAAG+AGG | 0.646167 | 8_1:+79532296 | Msa1217290:CDS |
| AAAATCACAGTAATCAAACC+AGG | 0.647796 | 8_1:-79532761 | None:intergenic |
| GGTTACTGGTTCAAGCAATG+TGG | 0.661828 | 8_1:+79532709 | Msa1217290:CDS |
| GGGGTAGAGAGTTGGGACAG+TGG | 0.661869 | 8_1:-79532379 | None:intergenic |
| TACACAGGAAGTGATTGTGA+AGG | 0.673700 | 8_1:+79532544 | Msa1217290:CDS |
| TTGGGATTCCTATTACGACA+CGG | 0.678110 | 8_1:-79532674 | None:intergenic |
| GAAATGGGTAACAGAAAACG+TGG | 0.703774 | 8_1:+79532273 | Msa1217290:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAATAAAAGAGAAAA+TGG | - | chr8_1:79532427-79532446 | None:intergenic | 15.0% |
| !!! | TTTTATTTTCATTCTTCATT+TGG | + | chr8_1:79532434-79532453 | Msa1217290:intron | 15.0% |
| !!! | TTTATTTTCATTCTTCATTT+GGG | + | chr8_1:79532435-79532454 | Msa1217290:intron | 15.0% |
| !! | ATAACAAATCAAACTATAAT+GGG | - | chr8_1:79532499-79532518 | None:intergenic | 15.0% |
| !! | AAAATAAAAGAGAAAATGGA+AGG | - | chr8_1:79532423-79532442 | None:intergenic | 20.0% |
| !! | TGCATTTAAACATGAAAAAT+TGG | - | chr8_1:79532464-79532483 | None:intergenic | 20.0% |
| !! | GATAACAAATCAAACTATAA+TGG | - | chr8_1:79532500-79532519 | None:intergenic | 20.0% |
| !!! | TTTGATTTGTTATCTGAAAT+GGG | + | chr8_1:79532506-79532525 | Msa1217290:intron | 20.0% |
| !!! | TTTGATTACTGTGATTTTTA+AGG | + | chr8_1:79532766-79532785 | Msa1217290:CDS | 20.0% |
| !! | TAACAAATCAGTTTCTGAAA+TGG | + | chr8_1:79532257-79532276 | Msa1217290:CDS | 25.0% |
| !!! | AACAAATCAGTTTCTGAAAT+GGG | + | chr8_1:79532258-79532277 | Msa1217290:CDS | 25.0% |
| !!! | GTTTGATTTGTTATCTGAAA+TGG | + | chr8_1:79532505-79532524 | Msa1217290:intron | 25.0% |
| !!! | TGAAATGGGTTTTTGTTTTT+AGG | + | chr8_1:79532520-79532539 | Msa1217290:intron | 25.0% |
| ! | TGAAGAAAAGAAGAAAATGT+TGG | + | chr8_1:79532592-79532611 | Msa1217290:CDS | 25.0% |
| ! | TAATGATGGTAATTTGATCT+TGG | + | chr8_1:79532835-79532854 | Msa1217290:CDS | 25.0% |
| GAATGATGTTAGTTACATTG+GGG | - | chr8_1:79532401-79532420 | None:intergenic | 30.0% | |
| GGAATGATGTTAGTTACATT+GGG | - | chr8_1:79532402-79532421 | None:intergenic | 30.0% | |
| AGGAATGATGTTAGTTACAT+TGG | - | chr8_1:79532403-79532422 | None:intergenic | 30.0% | |
| !!! | TTTTTGTTTTTAGGCTACAC+AGG | + | chr8_1:79532529-79532548 | Msa1217290:intron | 30.0% |
| AGAAAAGAAGAAAATGTTGG+AGG | + | chr8_1:79532595-79532614 | Msa1217290:CDS | 30.0% | |
| AAATCAAAGAAACCTGTTGA+AGG | + | chr8_1:79532632-79532651 | Msa1217290:CDS | 30.0% | |
| AATCAAAGAAACCTGTTGAA+GGG | + | chr8_1:79532633-79532652 | Msa1217290:CDS | 30.0% | |
| AAAATCACAGTAATCAAACC+AGG | - | chr8_1:79532764-79532783 | None:intergenic | 30.0% | |
| ! | GCTAATGTTTCTGTTAATGA+TGG | + | chr8_1:79532821-79532840 | Msa1217290:CDS | 30.0% |
| ! | TTCAACGTTTAGAACCTCTT+CGG | - | chr8_1:79532313-79532332 | None:intergenic | 35.0% |
| TAAACGTTGAACGTGAAAGA+AGG | + | chr8_1:79532322-79532341 | Msa1217290:CDS | 35.0% | |
| ATCAAAGAAACCTGTTGAAG+GGG | + | chr8_1:79532634-79532653 | Msa1217290:CDS | 35.0% | |
| AAATTAAAACCCCCTTCAAC+AGG | - | chr8_1:79532647-79532666 | None:intergenic | 35.0% | |
| !!! | TGAAGGGGGTTTTAATTTGT+TGG | + | chr8_1:79532649-79532668 | Msa1217290:CDS | 35.0% |
| GAATCCCAATTTCTCTGTTA+CGG | + | chr8_1:79532688-79532707 | Msa1217290:CDS | 35.0% | |
| GTAACCGTAACAGAGAAATT+GGG | - | chr8_1:79532695-79532714 | None:intergenic | 35.0% | |
| AGTAACCGTAACAGAGAAAT+TGG | - | chr8_1:79532696-79532715 | None:intergenic | 35.0% | |
| AATTTCTCTGTTACGGTTAC+TGG | + | chr8_1:79532695-79532714 | Msa1217290:CDS | 35.0% | |
| !! | TTTCTTTGGGATTGAATCTG+TGG | + | chr8_1:79532733-79532752 | Msa1217290:CDS | 35.0% |
| !! | GTTTTTGAAGAATGAAGCTG+AGG | + | chr8_1:79532790-79532809 | Msa1217290:CDS | 35.0% |
| GAAATGGGTAACAGAAAACG+TGG | + | chr8_1:79532273-79532292 | Msa1217290:CDS | 40.0% | |
| CACTACTACTAAAACCGAAG+AGG | + | chr8_1:79532296-79532315 | Msa1217290:CDS | 40.0% | |
| TTACATTGGGGTAGAGAGTT+GGG | - | chr8_1:79532389-79532408 | None:intergenic | 40.0% | |
| TACACAGGAAGTGATTGTGA+AGG | + | chr8_1:79532544-79532563 | Msa1217290:CDS | 40.0% | |
| GAAAATGTTGGAGGAGATGA+AGG | + | chr8_1:79532604-79532623 | Msa1217290:CDS | 40.0% | |
| TCAAAGAAACCTGTTGAAGG+GGG | + | chr8_1:79532635-79532654 | Msa1217290:CDS | 40.0% | |
| TTGGGATTCCTATTACGACA+CGG | - | chr8_1:79532677-79532696 | None:intergenic | 40.0% | |
| TCAAGCAATGTGGCTTTCTT+TGG | + | chr8_1:79532719-79532738 | Msa1217290:CDS | 40.0% | |
| CAAGCAATGTGGCTTTCTTT+GGG | + | chr8_1:79532720-79532739 | Msa1217290:CDS | 40.0% | |
| ATTGAATCTGTGGCTAAACC+TGG | + | chr8_1:79532743-79532762 | Msa1217290:CDS | 40.0% | |
| GTTACATTGGGGTAGAGAGT+TGG | - | chr8_1:79532390-79532409 | None:intergenic | 45.0% | |
| !! | TGTTGGTTCCGTGTCGTAAT+AGG | + | chr8_1:79532666-79532685 | Msa1217290:CDS | 45.0% |
| !! | GGTTACTGGTTCAAGCAATG+TGG | + | chr8_1:79532709-79532728 | Msa1217290:CDS | 45.0% |
| GATCTTGGCGATCACTGCTT+TGG | + | chr8_1:79532850-79532869 | Msa1217290:CDS | 50.0% | |
| ! | GAGTTGGGACAGTGGTAGTG+AGG | - | chr8_1:79532374-79532393 | None:intergenic | 55.0% |
| GGGGTAGAGAGTTGGGACAG+TGG | - | chr8_1:79532382-79532401 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 79532243 | 79532925 | 79532243 | ID=Msa1217290;Name=Msa1217290 |
| chr8_1 | mRNA | 79532243 | 79532925 | 79532243 | ID=Msa1217290-mRNA-1;Parent=Msa1217290;Name=Msa1217290-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|181 |
| chr8_1 | exon | 79532243 | 79532404 | 79532243 | ID=Msa1217290-mRNA-1:exon:27708;Parent=Msa1217290-mRNA-1 |
| chr8_1 | exon | 79532542 | 79532925 | 79532542 | ID=Msa1217290-mRNA-1:exon:27709;Parent=Msa1217290-mRNA-1 |
| chr8_1 | CDS | 79532243 | 79532404 | 79532243 | ID=Msa1217290-mRNA-1:cds;Parent=Msa1217290-mRNA-1 |
| chr8_1 | CDS | 79532542 | 79532925 | 79532542 | ID=Msa1217290-mRNA-1:cds;Parent=Msa1217290-mRNA-1 |
| Gene Sequence |
| Protein sequence |
