AlfalfaGEDB | Msa1217290

M. sativa cultivar zhongmu-4 / Msa1217290

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1217290	G7JJ33	90.055	181	18	0	1	181	1	181	4.09e-111	323

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TFs/TRs:

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Gene ID	Type	Classification
Msa1217290	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1217290	MtrunA17_Chr4g0066461	45.045	111	42	3	77	181	1	98	2.87e-20	80.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 34 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCACAGTAATCAAACC+AGG	0.647796	8_1:-79532761	None:intergenic
AAATCAAAGAAACCTGTTGA+AGG	0.526426	8_1:+79532632	Msa1217290:CDS
AACAAATCAGTTTCTGAAAT+GGG	0.410347	8_1:+79532258	Msa1217290:CDS
AATCAAAGAAACCTGTTGAA+GGG	0.548949	8_1:+79532633	Msa1217290:CDS
AATTTCTCTGTTACGGTTAC+TGG	0.183266	8_1:+79532695	Msa1217290:CDS
AGAAAAGAAGAAAATGTTGG+AGG	0.526690	8_1:+79532595	Msa1217290:CDS
AGTAACCGTAACAGAGAAAT+TGG	0.195649	8_1:-79532693	None:intergenic
ATCAAAGAAACCTGTTGAAG+GGG	0.470795	8_1:+79532634	Msa1217290:CDS
ATTGAATCTGTGGCTAAACC+TGG	0.515198	8_1:+79532743	Msa1217290:CDS
CAAGCAATGTGGCTTTCTTT+GGG	0.307847	8_1:+79532720	Msa1217290:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATGATGTTAGTTACATTG+GGG	-	chr8_1:79532401-79532420	None:intergenic	30.0%
GGAATGATGTTAGTTACATT+GGG	-	chr8_1:79532402-79532421	None:intergenic	30.0%
AGGAATGATGTTAGTTACAT+TGG	-	chr8_1:79532403-79532422	None:intergenic	30.0%
AGAAAAGAAGAAAATGTTGG+AGG	+	chr8_1:79532595-79532614	Msa1217290:CDS	30.0%
AAATCAAAGAAACCTGTTGA+AGG	+	chr8_1:79532632-79532651	Msa1217290:CDS	30.0%
AATCAAAGAAACCTGTTGAA+GGG	+	chr8_1:79532633-79532652	Msa1217290:CDS	30.0%
AAAATCACAGTAATCAAACC+AGG	-	chr8_1:79532764-79532783	None:intergenic	30.0%
TAAACGTTGAACGTGAAAGA+AGG	+	chr8_1:79532322-79532341	Msa1217290:CDS	35.0%
ATCAAAGAAACCTGTTGAAG+GGG	+	chr8_1:79532634-79532653	Msa1217290:CDS	35.0%
AAATTAAAACCCCCTTCAAC+AGG	-	chr8_1:79532647-79532666	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	79532243	79532925	79532243	ID=Msa1217290;Name=Msa1217290
chr8_1	mRNA	79532243	79532925	79532243	ID=Msa1217290-mRNA-1;Parent=Msa1217290;Name=Msa1217290-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|0\|0\|1\|1\|1\|2\|0\|181
chr8_1	exon	79532243	79532404	79532243	ID=Msa1217290-mRNA-1:exon:27708;Parent=Msa1217290-mRNA-1
chr8_1	exon	79532542	79532925	79532542	ID=Msa1217290-mRNA-1:exon:27709;Parent=Msa1217290-mRNA-1
chr8_1	CDS	79532243	79532404	79532243	ID=Msa1217290-mRNA-1:cds;Parent=Msa1217290-mRNA-1
chr8_1	CDS	79532542	79532925	79532542	ID=Msa1217290-mRNA-1:cds;Parent=Msa1217290-mRNA-1

Gene Sequence

>Msa1217290

ATGGAAGAGTACAGTAACAAATCAGTTTCTGAAATGGGTAACAGAAAACGTGGCACTACTACTAAAACCGAAGAGGTTCTAAACGTTGAACGTGAAAGAAGGATGAAAATGAAGCAAATGTTCACTCACCTCACTACCACTGTCCCAACTCTCTACCCCAATGCTACACAGGAAGTGATTGTGAAGGAAACAATCGAGTACATTAAGAAACTTGAAGAAAAGAAGAAAATGTTGGAGGAGATGAAGGAATCAAAATCAAAGAAACCTGTTGAAGGGGGTTTTAATTTGTTGGTTCCGTGTCGTAATAGGAATCCCAATTTCTCTGTTACGGTTACTGGTTCAAGCAATGTGGCTTTCTTTGGGATTGAATCTGTGGCTAAACCTGGTTTGATTACTGTGATTTTTAAGGTGTTTTTGAAGAATGAAGCTGAGGTTTTAGCTGCTAATGTTTCTGTTAATGATGGTAATTTGATCTTGGCGATCACTGCTTTGGTTCAAAATGCTGCTGCTATTGAGAAGATTAAGAGAGAGATTATTAGTTTGTAG

Protein sequence

>Msa1217290

MEEYSNKSVSEMGNRKRGTTTKTEEVLNVERERRMKMKQMFTHLTTTVPTLYPNATQEVIVKETIEYIKKLEEKKKMLEEMKESKSKKPVEGGFNLLVPCRNRNPNFSVTVTGSSNVAFFGIESVAKPGLITVIFKVFLKNEAEVLAANVSVNDGNLILAITALVQNAAAIEKIKREIISL*