AlfalfaGEDB | Msa1221740

M. sativa cultivar zhongmu-4 / Msa1221740

KEGG: K04733
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221740	XP_003610194.1	100.000	373	0	0	1	373	1	373	0.0	774

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221740	sp\|Q9LSC2\|Y3589_ARATH	51.592	314	138	3	6	313	6	311	6.40e-111	331

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221740	MtrunA17_Chr4g0072901	100.000	373	0	0	1	373	1	373	0.0	774
Msa1221740	MtrunA17_Chr2g0333181	62.769	325	115	1	27	345	25	349	8.25e-151	430
Msa1221740	MtrunA17_Chr7g0221191	48.867	309	151	3	6	310	360	665	7.42e-94	296
Msa1221740	MtrunA17_Chr6g0485761	47.368	304	153	3	17	315	269	570	3.03e-93	293
Msa1221740	MtrunA17_Chr6g0485721	48.464	293	145	3	29	318	85	374	6.56e-93	284
Msa1221740	MtrunA17_Chr8g0373501	42.424	330	166	5	29	337	324	650	3.83e-89	281
Msa1221740	MtrunA17_Chr5g0403891	41.813	342	178	5	30	353	244	582	1.23e-87	276
Msa1221740	MtrunA17_Chr2g0312561	44.147	299	166	1	28	325	29	327	2.54e-87	268
Msa1221740	MtrunA17_Chr3g0144481	40.643	342	186	7	30	366	77	406	8.90e-87	270
Msa1221740	MtrunA17_Chr7g0251771	43.689	309	170	4	8	313	17	324	6.66e-86	265

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1221740	AT1G52540.1	53.968	315	131	3	4	313	5	310	3.79e-117	344
Msa1221740	AT1G52540.2	56.597	288	124	1	26	313	21	307	1.19e-116	343
Msa1221740	AT3G15890.1	51.592	314	138	3	6	313	6	311	6.50e-112	331
Msa1221740	AT1G70460.1	42.857	378	198	6	1	364	313	686	1.70e-94	297
Msa1221740	AT5G38560.1	42.857	364	187	5	21	365	318	679	7.26e-92	290
Msa1221740	AT3G09010.1	42.444	311	177	2	7	317	12	320	1.63e-90	278
Msa1221740	AT3G24550.1	46.441	295	152	2	26	316	264	556	1.13e-89	283
Msa1221740	AT1G10620.1	42.398	342	185	4	30	361	358	697	1.92e-89	284
Msa1221740	AT3G26940.1	45.031	322	167	4	3	314	31	352	4.74e-89	275
Msa1221740	AT1G26150.1	47.405	289	146	2	26	310	414	700	1.93e-88	283

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Find 86 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAAAATCAGCAACTAG+AGG	0.669735	8_1:-85597033	None:intergenic
AAAGATCCTTACAAGAAAAT+TGG	0.297509	8_1:+85597585	Msa1221740:CDS
AAAGGACAAGAACCTGATCA+AGG	0.510324	8_1:+85597429	Msa1221740:CDS
AACCTTCCTTTGGTTATCAA+AGG	0.477215	8_1:-85597270	None:intergenic
AAGAACCAGTGATGGGCTAC+AGG	0.485316	8_1:+85596151	Msa1221740:CDS
AAGAGAATGAGCATTGCAAT+TGG	0.475050	8_1:+85596599	Msa1221740:CDS
AAGCTTGGTGAAGGTGGGTT+TGG	0.300232	8_1:+85596113	Msa1221740:CDS
AAGGACAAGAACCTGATCAA+GGG	0.612248	8_1:+85597430	Msa1221740:CDS
AATGAATTCAAAGGCAGAGA+TGG	0.547946	8_1:+85596400	Msa1221740:CDS
ACCCGTGTTAAGGGTACCTT+AGG	0.529977	8_1:+85597093	Msa1221740:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCAGTGATGATTATAAGCT+TGG	+	chr8_1:85596098-85596117	Msa1221740:CDS	30.0%
TTGCAATTAACTCTCTTCAT+TGG	+	chr8_1:85596345-85596364	Msa1221740:intron	30.0%
ATACTAACAAGATAGTATGC+AGG	+	chr8_1:85596876-85596895	Msa1221740:intron	30.0%
TTCAGTGATGATTATAAGCT+TGG	+	chr8_1:85596098-85596117	Msa1221740:CDS	30.0%
TTGCAATTAACTCTCTTCAT+TGG	+	chr8_1:85596345-85596364	Msa1221740:intron	30.0%
ATACTAACAAGATAGTATGC+AGG	+	chr8_1:85596876-85596895	Msa1221740:intron	30.0%
TTAGCTACACCATATGATGT+TGG	-	chr8_1:85596026-85596045	None:intergenic	35.0%
ATGGTGTAGCTAACAATTCT+TGG	+	chr8_1:85596033-85596052	Msa1221740:CDS	35.0%
AATGAATTCAAAGGCAGAGA+TGG	+	chr8_1:85596400-85596419	Msa1221740:CDS	35.0%
GGTTAGGCACAAAAATTTGT+TGG	+	chr8_1:85596454-85596473	Msa1221740:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	85595856	85597611	85595856	ID=Msa1221740;Name=Msa1221740
chr8_1	mRNA	85595856	85597611	85595856	ID=Msa1221740-mRNA-1;Parent=Msa1221740;Name=Msa1221740-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0\|0\|0\|1\|1\|1\|4\|0\|373
chr8_1	exon	85595856	85595902	85595856	ID=Msa1221740-mRNA-1:exon:30779;Parent=Msa1221740-mRNA-1
chr8_1	exon	85596022	85596172	85596022	ID=Msa1221740-mRNA-1:exon:30780;Parent=Msa1221740-mRNA-1
chr8_1	exon	85596377	85596639	85596377	ID=Msa1221740-mRNA-1:exon:30781;Parent=Msa1221740-mRNA-1
chr8_1	exon	85596951	85597611	85596951	ID=Msa1221740-mRNA-1:exon:30782;Parent=Msa1221740-mRNA-1
chr8_1	CDS	85595856	85595902	85595856	ID=Msa1221740-mRNA-1:cds;Parent=Msa1221740-mRNA-1
chr8_1	CDS	85596022	85596172	85596022	ID=Msa1221740-mRNA-1:cds;Parent=Msa1221740-mRNA-1
chr8_1	CDS	85596377	85596639	85596377	ID=Msa1221740-mRNA-1:cds;Parent=Msa1221740-mRNA-1
chr8_1	CDS	85596951	85597611	85596951	ID=Msa1221740-mRNA-1:cds;Parent=Msa1221740-mRNA-1

Gene Sequence

>Msa1221740

ATGGGATCTTCCTTGAGTTGTTGTGGCTCGGAGAAGGTTGATGAAGTGCCAACATCATATGGTGTAGCTAACAATTCTTGGAGAATTTTCACATACAAGGAGTTGCATACAGCTACAAACGGGTTCAGTGATGATTATAAGCTTGGTGAAGGTGGGTTTGGGAGTGTCTATTGGGGAAGAACCAGTGATGGGCTACAGATAGCAGTGAAGAAATTGAAAGCAATGAATTCAAAGGCAGAGATGGAATTTGCTGTAGAAGTTGAAGTACTTGGAAGGGTTAGGCACAAAAATTTGTTGGGTCTAAGGGGATATTGTGTTGGCGATGATCAAAGGCTTATTGTCTACGATTACATGCCAAATCTAAGTCTGCTTTCTCATCTCCATGGTCAATATGCTGGTGAAGTGCAACTCAACTGGCAAAAGAGAATGAGCATTGCAATTGGCTCTGCTGAAGGCATTTTGTACTTGCACCATGAGGTCACACCCCACATCATCCATAGGGACATCAAAGCTAGTAATGTTTTGCTTGATTCAGATTTTGTGCCTCTAGTTGCTGATTTTGGTTTTGCAAAGCTAATCCCTGAAGGAGTTAGCCACATGACAACCCGTGTTAAGGGTACCTTAGGATATTTAGCACCTGAGTATGCTATGTGGGGAAAAGTTTCTGAAAGCTGTGATGTCTATAGTTTTGGAATTCTTCTGTTAGAGCTTGTAACTGGTAGAAAGCCCATAGAAAAACTACCTGGTGGGCTTAAGAGAACAATAACTGAATGGGCTGAGCCTTTGATAACCAAAGGAAGGTTTAGGGATATGGTTGATCCAAAACTCAGAGGAAACTTTGATGAAAATCAAGTTAAACAAACAGTTAATGTGGCTGCTCTTTGTGTGCAAAGTGAACCTGAGAAACGACCAAACATGAAGCAAGTTGTTAGTCTTTTGAAAGGACAAGAACCTGATCAAGGGAAAGTGACTAAGATGAGAATAGACAGTGTCAAATATAATGATGAATTATTGGCACTTGATCAACCTAGTGATGATGATTATGATGGAAACAGTAGCTATGGTGTTTTTAGTGCTATTGATGTCCAAAAGATGAAAGATCCTTACAAGAAAATTGGCTAA

Protein sequence

>Msa1221740

MGSSLSCCGSEKVDEVPTSYGVANNSWRIFTYKELHTATNGFSDDYKLGEGGFGSVYWGRTSDGLQIAVKKLKAMNSKAEMEFAVEVEVLGRVRHKNLLGLRGYCVGDDQRLIVYDYMPNLSLLSHLHGQYAGEVQLNWQKRMSIAIGSAEGILYLHHEVTPHIIHRDIKASNVLLDSDFVPLVADFGFAKLIPEGVSHMTTRVKGTLGYLAPEYAMWGKVSESCDVYSFGILLLELVTGRKPIEKLPGGLKRTITEWAEPLITKGRFRDMVDPKLRGNFDENQVKQTVNVAALCVQSEPEKRPNMKQVVSLLKGQEPDQGKVTKMRIDSVKYNDELLALDQPSDDDYDGNSSYGVFSAIDVQKMKDPYKKIG*