AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1224420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1224420 MtrunA17_Chr4g0076321 93.750 80 5 0 1 80 51 130 1.72e-52 159
Msa1224420 MtrunA17_Chr2g0290491 73.750 80 18 1 1 80 49 125 1.47e-37 123
Msa1224420 MtrunA17_Chr3g0102351 84.127 63 10 0 1 63 59 121 9.83e-37 121
Msa1224420 MtrunA17_Chr5g0446491 80.303 66 13 0 1 66 55 120 6.83e-36 119
Msa1224420 MtrunA17_Chr8g0384451 80.952 63 12 0 1 63 72 134 3.84e-35 118
Msa1224420 MtrunA17_Chr8g0382931 80.303 66 13 0 1 66 60 125 1.43e-34 116
Msa1224420 MtrunA17_Chr1g0185911 73.077 78 18 2 1 77 61 136 1.94e-33 112
Msa1224420 MtrunA17_Chr7g0263601 65.854 82 25 1 1 79 58 139 2.86e-33 112
Msa1224420 MtrunA17_Chr2g0296321 73.016 63 17 0 1 63 98 160 3.42e-32 112
Msa1224420 MtrunA17_Chr1g0195851 71.429 63 18 0 1 63 68 130 3.64e-32 110
Msa1224420 MtrunA17_Chr4g0067091 73.333 60 16 0 1 60 54 113 2.24e-31 108
Msa1224420 MtrunA17_Chr4g0076381 66.667 63 21 0 1 63 90 152 2.19e-29 104
Msa1224420 MtrunA17_Chr1g0191981 63.333 60 22 0 1 60 36 95 2.15e-26 93.6
Msa1224420 MtrunA17_Chr1g0165041 57.143 63 27 0 1 63 38 100 1.54e-24 90.5
Msa1224420 MtrunA17_Chr1g0158951 54.098 61 28 0 4 64 24 84 6.60e-19 74.3
Msa1224420 MtrunA17_Chr1g0159271 51.667 60 29 0 2 61 20 79 3.93e-16 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1224420 AT4G14540.1 84.127 63 10 0 1 63 53 115 5.02e-36 119
Msa1224420 AT5G47640.1 81.250 64 12 0 1 64 59 122 1.24e-35 119
Msa1224420 AT2G38880.3 66.667 81 26 1 1 80 53 133 4.11e-34 114
Msa1224420 AT2G38880.2 66.667 81 26 1 1 80 53 133 6.27e-34 113
Msa1224420 AT2G38880.1 66.667 81 26 1 1 80 53 133 6.27e-34 113
Msa1224420 AT2G38880.5 66.667 81 26 1 1 80 53 133 6.27e-34 113
Msa1224420 AT3G53340.5 79.032 62 13 0 1 62 61 122 9.13e-34 114
Msa1224420 AT3G53340.1 79.032 62 13 0 1 62 61 122 9.13e-34 114
Msa1224420 AT2G37060.2 79.032 62 13 0 1 62 62 123 3.11e-33 112
Msa1224420 AT2G37060.3 79.032 62 13 0 1 62 62 123 3.11e-33 112
Msa1224420 AT2G37060.1 79.032 62 13 0 1 62 62 123 3.11e-33 112
Msa1224420 AT2G13570.1 76.190 63 15 0 1 63 68 130 1.70e-32 112
Msa1224420 AT3G53340.4 77.966 59 13 0 1 59 61 119 2.71e-32 108
Msa1224420 AT2G38880.4 79.661 59 12 0 1 59 53 111 7.13e-32 107
Msa1224420 AT3G53340.3 78.333 60 13 0 1 60 61 120 8.33e-32 108
Msa1224420 AT3G53340.2 78.333 60 13 0 1 60 61 120 8.33e-32 108
Msa1224420 AT2G47810.1 73.016 63 17 0 1 63 83 145 9.16e-32 108
Msa1224420 AT2G38880.9 79.661 59 12 0 1 59 53 111 1.14e-31 108
Msa1224420 AT2G38880.10 79.661 59 12 0 1 59 53 111 1.14e-31 108
Msa1224420 AT2G38880.6 79.661 59 12 0 1 59 53 111 1.14e-31 108
Msa1224420 AT2G38880.7 63.750 80 28 1 1 80 53 131 3.49e-31 106
Msa1224420 AT2G38880.11 79.661 59 12 0 1 59 53 111 3.86e-31 107
Msa1224420 AT2G38880.8 79.661 59 12 0 1 59 53 111 3.86e-31 107
Msa1224420 AT5G47670.2 63.636 66 24 0 1 66 61 126 4.08e-29 103
Msa1224420 AT5G47670.1 63.636 66 24 0 1 66 90 155 7.36e-29 103
Msa1224420 AT5G47670.3 63.636 66 24 0 1 66 90 155 7.36e-29 103
Msa1224420 AT1G21970.1 55.224 67 30 0 1 67 91 157 5.89e-26 95.9
Msa1224420 AT1G09030.1 53.846 78 33 1 1 75 35 112 1.10e-25 92.4

Find 64 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAGTGACTAACTTCTT+TGG 0.285324 8_1:+89352786 Msa1224420:three_prime_UTR
ACTAACATAAGGGTCATTAT+TGG 0.309434 8_1:-89353083 None:intergenic
ACTAGTTTCATCTATCATAT+TGG 0.309640 8_1:-89352998 None:intergenic
TAATGACCCTTATGTTAGTT+TGG 0.309793 8_1:+89353087 Msa1224420:three_prime_UTR
GCCTTGTGTGTTACCTTTGT+TGG 0.322361 8_1:+89352339 Msa1224420:three_prime_UTR
TCAAAGAAAGGAACAAAGAT+AGG 0.336530 8_1:-89352836 None:intergenic
TTCTTGGGTGTTGCTGCCTC+AGG 0.354609 8_1:+89352149 Msa1224420:three_prime_UTR
GGGTTACCTTCACAAGTATA+GGG 0.382046 8_1:+89349817 Msa1224420:CDS
CTTTGACGAAATAATGCATC+AGG 0.389390 8_1:+89352853 Msa1224420:three_prime_UTR
AGAATATGCAGAGCCTCTTA+AGG 0.393028 8_1:+89349796 Msa1224420:CDS
TCAACAACCGGCTAAGCAAC+TGG 0.398538 8_1:+89352399 Msa1224420:three_prime_UTR
TGAGTGCAGACTGTGTTGTT+TGG 0.408300 8_1:+89352363 Msa1224420:three_prime_UTR
TGAACTTCTGATGCAATTGT+AGG 0.413663 8_1:+89353060 Msa1224420:three_prime_UTR
AATATAACTCCTCCGTTCTC+TGG 0.417900 8_1:-89352427 None:intergenic
CCTTGTGTGTTACCTTTGTT+GGG 0.419797 8_1:+89352340 Msa1224420:three_prime_UTR
AAAAGCACCAAACTAACATA+AGG 0.428110 8_1:-89353094 None:intergenic
TGTTGAAGCGTTCAGTTAAC+CGG 0.428959 8_1:+89352098 Msa1224420:three_prime_UTR
AGGGTTACCTTCACAAGTAT+AGG 0.429699 8_1:+89349816 Msa1224420:CDS
GGTGTTTCCAGTTGCTTAGC+CGG 0.429936 8_1:-89352406 None:intergenic
TAACCGGTAGGTCGCGTGAC+TGG 0.438081 8_1:+89352114 Msa1224420:three_prime_UTR
GTAAAATAACAAACTTCATC+AGG 0.441960 8_1:-89352932 None:intergenic
TGCATTATTTCGTCAAAGAA+AGG 0.443489 8_1:-89352848 None:intergenic
TAATGCATCAGGTCACTTGT+TGG 0.443570 8_1:+89352864 Msa1224420:three_prime_UTR
AAGAAGTTAGTCACTTCATC+AGG 0.458763 8_1:-89352783 None:intergenic
GAGTTCATAAGCTTCATAAC+AGG 0.460674 8_1:+89349686 Msa1224420:CDS
GAGAAGAGGAAGACAATAAA+CGG 0.477283 8_1:+89349734 Msa1224420:CDS
ATGCAATGTACATCAACAAC+CGG 0.477504 8_1:+89352387 Msa1224420:three_prime_UTR
TTGTGAAGGTAACCCTTAAG+AGG 0.483388 8_1:-89349809 None:intergenic
CAATTTCCCTATACTTGTGA+AGG 0.485169 8_1:-89349823 None:intergenic
GAATATGCAGAGCCTCTTAA+GGG 0.488554 8_1:+89349797 Msa1224420:CDS
TGCTCAGTAATATTAGATAT+AGG 0.496069 8_1:-89352729 None:intergenic
GTAGGTCGCGTGACTGGAAG+TGG 0.505525 8_1:+89352120 Msa1224420:three_prime_UTR
CTGAATGAAACAGCTTCAAC+CGG 0.508703 8_1:-89352281 None:intergenic
ATCGTCGAAGACTATTTAAG+AGG 0.511190 8_1:+89352466 Msa1224420:three_prime_UTR
TGTCTTGGACTCTTCTAGGA+TGG 0.512896 8_1:+89352686 Msa1224420:three_prime_UTR
GAATATGATTGGTAGTAGTA+AGG 0.514281 8_1:+89349868 Msa1224420:CDS
CACAACAAGTAAAACATGAC+TGG 0.517648 8_1:-89353039 None:intergenic
TTTGTTAGTCTAATTAGGAC+CGG 0.529197 8_1:+89352262 Msa1224420:three_prime_UTR
GCCACAGGTTTCCGCTGCAA+AGG 0.529470 8_1:+89352659 Msa1224420:intron
TGTTTCATTCAGTTTAGAGA+GGG 0.531159 8_1:+89352292 Msa1224420:three_prime_UTR
CACAAGTATAGGGAAATTGA+AGG 0.531636 8_1:+89349827 Msa1224420:CDS
AAAGCACCAAACTAACATAA+GGG 0.543668 8_1:-89353093 None:intergenic
TTTCATCTATCATATTGGAC+AGG 0.544625 8_1:-89352993 None:intergenic
TTAACTGAACGCTTCAACAG+AGG 0.545048 8_1:-89352094 None:intergenic
AAAAGTGTCAGAAAGAGAAG+AGG 0.551950 8_1:+89349720 Msa1224420:CDS
CAAAGAAAGGAACAAAGATA+GGG 0.558390 8_1:-89352835 None:intergenic
TTCAAAGCCAAGTGTAGTCA+TGG 0.563942 8_1:-89349774 None:intergenic
GTGTGGGCCATGACTACACT+TGG 0.565546 8_1:+89349767 Msa1224420:CDS
CTTCCAGTCACGCGACCTAC+CGG 0.577781 8_1:-89352117 None:intergenic
CCCAACAAAGGTAACACACA+AGG 0.581016 8_1:-89352340 None:intergenic
CTGTTTCATTCAGTTTAGAG+AGG 0.581488 8_1:+89352291 Msa1224420:three_prime_UTR
GAGGATGTGTTGCTGTCACA+TGG 0.597743 8_1:+89352315 Msa1224420:three_prime_UTR
CAGGAAAGTCACGAGTCGAT+AGG 0.608965 8_1:-89352017 None:intergenic
TCATTCAGTTTAGAGAGGGG+AGG 0.610045 8_1:+89352296 Msa1224420:three_prime_UTR
GTTTCATTCAGTTTAGAGAG+GGG 0.619294 8_1:+89352293 Msa1224420:three_prime_UTR
CAACTGGAAACACCAGAGAA+CGG 0.632876 8_1:+89352415 Msa1224420:three_prime_UTR
GAAGCGTTCAGTTAACCGGT+AGG 0.641958 8_1:+89352102 Msa1224420:three_prime_UTR
GGTAGTAGTAAGGAGCAGCA+AGG 0.645799 8_1:+89349878 Msa1224420:CDS
TAGGTCGCGTGACTGGAAGT+GGG 0.646015 8_1:+89352121 Msa1224420:three_prime_UTR
TAAACGGTGATGATCTTGTG+TGG 0.661494 8_1:+89349750 Msa1224420:CDS
AGTCTGCACTCACCCAACAA+AGG 0.683628 8_1:-89352352 None:intergenic
AAACGGTGATGATCTTGTGT+GGG 0.691215 8_1:+89349751 Msa1224420:CDS
GCCACATCACCTAAAACCTG+AGG 0.693839 8_1:-89352165 None:intergenic
CTGGAAACACCAGAGAACGG+AGG 0.752253 8_1:+89352418 Msa1224420:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAGAATTATAAATATATT+AGG + chr8_1:89351773-89351792 Msa1224420:intron 10.0%
!! ATTCTTCAAAAAAAAAAATA+AGG - chr8_1:89351802-89351821 None:intergenic 10.0%
!!! TTTTTTTTTGAAGAATTATT+AGG + chr8_1:89351806-89351825 Msa1224420:intron 10.0%
!!! TGTTATTTTACATTTAATTT+AGG + chr8_1:89352944-89352963 Msa1224420:three_prime_UTR 10.0%
!!! AATTTTTCTATGAATATGAT+TGG + chr8_1:89349857-89349876 Msa1224420:CDS 15.0%
!!! AATTAATTTTAGTGATGATT+TGG + chr8_1:89350133-89350152 Msa1224420:intron 15.0%
!!! GGTTTTTCTTAAATTATTAT+CGG + chr8_1:89350288-89350307 Msa1224420:intron 15.0%
!!! CTATTATTTGTATTCTTTTA+TGG - chr8_1:89350546-89350565 None:intergenic 15.0%
!! TTCCTAGTATAATATATAAA+TGG + chr8_1:89350577-89350596 Msa1224420:intron 15.0%
!! ATCCATTTATATATTATACT+AGG - chr8_1:89350582-89350601 None:intergenic 15.0%
!! ACAAATCAATATATAAACAT+GGG - chr8_1:89351074-89351093 None:intergenic 15.0%
!! TACAAATCAATATATAAACA+TGG - chr8_1:89351075-89351094 None:intergenic 15.0%
!! CAGAAAATTAATCAAATTTA+AGG - chr8_1:89351291-89351310 None:intergenic 15.0%
!! TAAAATTGTCATAAATGTAT+GGG + chr8_1:89351704-89351723 Msa1224420:intron 15.0%
!!! TTTAGTTTTTATTGTAGTTT+AGG + chr8_1:89351984-89352003 Msa1224420:intron 15.0%
!!! TTAATTTATGCCTCTTTTTT+AGG + chr8_1:89349967-89349986 Msa1224420:intron 20.0%
!! TATGATTACATTGAATTATG+TGG + chr8_1:89350267-89350286 Msa1224420:intron 20.0%
!!! TAAGTTGCGATTTAAATTTT+AGG + chr8_1:89351101-89351120 Msa1224420:intron 20.0%
!!! AAGTTGCGATTTAAATTTTA+GGG + chr8_1:89351102-89351121 Msa1224420:intron 20.0%
!! ATCTAAACAAATCACAAAAT+CGG - chr8_1:89351179-89351198 None:intergenic 20.0%
!!! ATTTGAGTTGTCTTAAAATT+GGG + chr8_1:89351203-89351222 Msa1224420:intron 20.0%
!! TCAAATTTGTGATTCTAATT+TGG + chr8_1:89351261-89351280 Msa1224420:intron 20.0%
!! CAAATTTGTGATTCTAATTT+GGG + chr8_1:89351262-89351281 Msa1224420:intron 20.0%
!! AAATCCTAAATCGAAAAAAT+TGG - chr8_1:89351314-89351333 None:intergenic 20.0%
!!! TTTTCGATTTAGGATTTATT+TGG + chr8_1:89351317-89351336 Msa1224420:intron 20.0%
!! CTAAAATTGTCATAAATGTA+TGG + chr8_1:89351703-89351722 Msa1224420:intron 20.0%
!!! TTCCTATTAAAATGTGTTTT+AGG + chr8_1:89351930-89351949 Msa1224420:intron 20.0%
!!! TGAATTAATGTCTCTTTTTA+TGG + chr8_1:89351953-89351972 Msa1224420:intron 20.0%
!! ACAATAAAAACTAAAATCAG+AGG - chr8_1:89351979-89351998 None:intergenic 20.0%
!!! ACATTTTTGTTAGTCTAATT+AGG + chr8_1:89352257-89352276 Msa1224420:three_prime_UTR 20.0%
!! ATTGTAGTATATGTTGAAAT+TGG + chr8_1:89352506-89352525 Msa1224420:intron 20.0%
! ACATTAAAAGCCTAAAAAAG+AGG - chr8_1:89349980-89349999 None:intergenic 25.0%
! ACAAAACAACAATAACAAAC+TGG - chr8_1:89350022-89350041 None:intergenic 25.0%
! TTCAATGTAATCATATGTGT+CGG - chr8_1:89350262-89350281 None:intergenic 25.0%
! AAAGAACCTAAATAATTGAG+TGG + chr8_1:89350488-89350507 Msa1224420:intron 25.0%
!!! AAATTTTAGGGTTTCTGATT+TGG + chr8_1:89351114-89351133 Msa1224420:intron 25.0%
!!! AATTTTAGGGTTTCTGATTT+GGG + chr8_1:89351115-89351134 Msa1224420:intron 25.0%
!!! GATTTGAGTTGTCTTAAAAT+TGG + chr8_1:89351202-89351221 Msa1224420:intron 25.0%
!!! TTTGAGTTGTCTTAAAATTG+GGG + chr8_1:89351204-89351223 Msa1224420:intron 25.0%
! AGAATCACAAATTTGAAGAT+GGG - chr8_1:89351257-89351276 None:intergenic 25.0%
! TAGAATCACAAATTTGAAGA+TGG - chr8_1:89351258-89351277 None:intergenic 25.0%
! ACACAAATACCATAAAGATA+GGG - chr8_1:89351399-89351418 None:intergenic 25.0%
! TACACAAATACCATAAAGAT+AGG - chr8_1:89351400-89351419 None:intergenic 25.0%
! TGATAGCATTTATCTGTTAA+TGG - chr8_1:89351508-89351527 None:intergenic 25.0%
! TTAGAACAAAATACCAAAGT+TGG - chr8_1:89351687-89351706 None:intergenic 25.0%
!!! ATGGTATTTTAGTCTCTATT+GGG + chr8_1:89351849-89351868 Msa1224420:intron 25.0%
!!! CACCTAAAACACATTTTAAT+AGG - chr8_1:89351935-89351954 None:intergenic 25.0%
! CCAATAAAGCAATTGATATT+TGG - chr8_1:89352536-89352555 None:intergenic 25.0%
! CCAAATATCAATTGCTTTAT+TGG + chr8_1:89352533-89352552 Msa1224420:intron 25.0%
! TCACATGTGAAATTGATATT+TGG - chr8_1:89352563-89352582 None:intergenic 25.0%
! TAAAGCAATAAAGCATGTTA+CGG - chr8_1:89352592-89352611 None:intergenic 25.0%
! TGCTCAGTAATATTAGATAT+AGG - chr8_1:89352732-89352751 None:intergenic 25.0%
! GTAAAATAACAAACTTCATC+AGG - chr8_1:89352935-89352954 None:intergenic 25.0%
!! ACTAGTTTCATCTATCATAT+TGG - chr8_1:89353001-89353020 None:intergenic 25.0%
! GAATATGATTGGTAGTAGTA+AGG + chr8_1:89349868-89349887 Msa1224420:CDS 30.0%
GTTGCAAATATAATCAACCT+TGG - chr8_1:89349922-89349941 None:intergenic 30.0%
!! GTGATGATTTGGTATACATA+TGG + chr8_1:89350144-89350163 Msa1224420:intron 30.0%
TTCCTTTCAGGAAATAAGAT+TGG + chr8_1:89350175-89350194 Msa1224420:intron 30.0%
! TACCAATCTTATTTCCTGAA+AGG - chr8_1:89350180-89350199 None:intergenic 30.0%
!! TACTTTTTATCTGTGAAGCA+CGG - chr8_1:89350347-89350366 None:intergenic 30.0%
!! TACGATTTTGACAGAAACTA+CGG - chr8_1:89350409-89350428 None:intergenic 30.0%
ATGTAGCCACTCAATTATTT+AGG - chr8_1:89350497-89350516 None:intergenic 30.0%
CATGAACTAGTTACCAATTA+AGG - chr8_1:89350522-89350541 None:intergenic 30.0%
ATAAGAGATGTTGCTCAAAA+AGG + chr8_1:89350600-89350619 Msa1224420:intron 30.0%
TGCTCAAAAAGGAAAAAGAT+TGG + chr8_1:89350611-89350630 Msa1224420:intron 30.0%
AAAATACCCATGTCACATAA+GGG - chr8_1:89350671-89350690 None:intergenic 30.0%
AAAAATACCCATGTCACATA+AGG - chr8_1:89350672-89350691 None:intergenic 30.0%
GAGAAGAAGAAAGTGAATAA+GGG - chr8_1:89350737-89350756 None:intergenic 30.0%
AGAGAAGAAGAAAGTGAATA+AGG - chr8_1:89350738-89350757 None:intergenic 30.0%
! AAGATTCTGAGAAACAAAAG+TGG - chr8_1:89350985-89351004 None:intergenic 30.0%
!!! ATTTTAGGGTTTCTGATTTG+GGG + chr8_1:89351116-89351135 Msa1224420:intron 30.0%
! TGTCTTAAAATTGGGGATTT+TGG + chr8_1:89351211-89351230 Msa1224420:intron 30.0%
TTGAAGATGGGTACATAAAT+TGG - chr8_1:89351245-89351264 None:intergenic 30.0%
!!! TCTGCCAATTTTTTCGATTT+AGG + chr8_1:89351307-89351326 Msa1224420:intron 30.0%
AATAATCCTCCCTATCTTTA+TGG + chr8_1:89351387-89351406 Msa1224420:intron 30.0%
! TTAACTCTGTCTGTAGATTT+TGG + chr8_1:89351446-89351465 Msa1224420:intron 30.0%
! TCAATGAACTGACTTATTCT+CGG + chr8_1:89351547-89351566 Msa1224420:intron 30.0%
GTGAGAAGAAGTAATATCTT+TGG + chr8_1:89351641-89351660 Msa1224420:intron 30.0%
AGTACATAAATCTCCAACTT+TGG + chr8_1:89351671-89351690 Msa1224420:intron 30.0%
!!! CATGGTATTTTAGTCTCTAT+TGG + chr8_1:89351848-89351867 Msa1224420:intron 30.0%
ATCACTGACAAAAATATTGC+AGG - chr8_1:89352039-89352058 None:intergenic 30.0%
AGACTAACAAAAATGTTGCA+GGG - chr8_1:89352253-89352272 None:intergenic 30.0%
TAGACTAACAAAAATGTTGC+AGG - chr8_1:89352254-89352273 None:intergenic 30.0%
! TTTGTTAGTCTAATTAGGAC+CGG + chr8_1:89352262-89352281 Msa1224420:three_prime_UTR 30.0%
TGTTTCATTCAGTTTAGAGA+GGG + chr8_1:89352292-89352311 Msa1224420:three_prime_UTR 30.0%
! AAGACTATTTAAGAGGTATG+TGG + chr8_1:89352473-89352492 Msa1224420:intron 30.0%
AAAGCAATAAAGCATGTTAC+GGG - chr8_1:89352591-89352610 None:intergenic 30.0%
CAAAGAAAGGAACAAAGATA+GGG - chr8_1:89352838-89352857 None:intergenic 30.0%
TCAAAGAAAGGAACAAAGAT+AGG - chr8_1:89352839-89352858 None:intergenic 30.0%
TGCATTATTTCGTCAAAGAA+AGG - chr8_1:89352851-89352870 None:intergenic 30.0%
TTTCATCTATCATATTGGAC+AGG - chr8_1:89352996-89353015 None:intergenic 30.0%
ACTAACATAAGGGTCATTAT+TGG - chr8_1:89353086-89353105 None:intergenic 30.0%
TAATGACCCTTATGTTAGTT+TGG + chr8_1:89353087-89353106 Msa1224420:three_prime_UTR 30.0%
! AAAGCACCAAACTAACATAA+GGG - chr8_1:89353096-89353115 None:intergenic 30.0%
!! AAAAGCACCAAACTAACATA+AGG - chr8_1:89353097-89353116 None:intergenic 30.0%
GAGTTCATAAGCTTCATAAC+AGG + chr8_1:89349686-89349705 Msa1224420:CDS 35.0%
!! AAAAGTGTCAGAAAGAGAAG+AGG + chr8_1:89349720-89349739 Msa1224420:CDS 35.0%
GAGAAGAGGAAGACAATAAA+CGG + chr8_1:89349734-89349753 Msa1224420:CDS 35.0%
CAATTTCCCTATACTTGTGA+AGG - chr8_1:89349826-89349845 None:intergenic 35.0%
CACAAGTATAGGGAAATTGA+AGG + chr8_1:89349827-89349846 Msa1224420:CDS 35.0%
!! AGTATTGATCGATTCTTCCA+AGG + chr8_1:89349902-89349921 Msa1224420:intron 35.0%
!!! ATGGCGTTTTTTTTCCTTTC+AGG + chr8_1:89350163-89350182 Msa1224420:intron 35.0%
GGAAATAAGATTGGTAAGCA+CGG + chr8_1:89350184-89350203 Msa1224420:intron 35.0%
TCCTCGAATTAAGTGTATCT+TGG + chr8_1:89350211-89350230 Msa1224420:intron 35.0%
ACCAAGATACACTTAATTCG+AGG - chr8_1:89350215-89350234 None:intergenic 35.0%
! ACTTTTTATCTGTGAAGCAC+GGG - chr8_1:89350346-89350365 None:intergenic 35.0%
TTGAGTATCAGGAAGTTTGA+CGG - chr8_1:89350448-89350467 None:intergenic 35.0%
AGTGGCTACATTTCCTTAAT+TGG + chr8_1:89350506-89350525 Msa1224420:intron 35.0%
AAATACCCATGTCACATAAG+GGG - chr8_1:89350670-89350689 None:intergenic 35.0%
GAAGAAAGTGAATAAGGGAA+TGG - chr8_1:89350732-89350751 None:intergenic 35.0%
! ATTCTGAGAAACAAAAGTGG+CGG - chr8_1:89350982-89351001 None:intergenic 35.0%
TTGTGATTCACTAACCTTTC+TGG - chr8_1:89351049-89351068 None:intergenic 35.0%
CAAATACCATAAAGATAGGG+AGG - chr8_1:89351396-89351415 None:intergenic 35.0%
!!! CTGTCTGTAGATTTTGGTTT+TGG + chr8_1:89351452-89351471 Msa1224420:intron 35.0%
CATTTCAACCCTCACATATA+GGG - chr8_1:89351622-89351641 None:intergenic 35.0%
ACATTTCAACCCTCACATAT+AGG - chr8_1:89351623-89351642 None:intergenic 35.0%
GATGTTATATGTAGCTCACT+TGG + chr8_1:89351726-89351745 Msa1224420:intron 35.0%
TACCATGCAGCTGATTAATA+AGG - chr8_1:89351835-89351854 None:intergenic 35.0%
CTGTTTCATTCAGTTTAGAG+AGG + chr8_1:89352291-89352310 Msa1224420:three_prime_UTR 35.0%
GTTTCATTCAGTTTAGAGAG+GGG + chr8_1:89352293-89352312 Msa1224420:three_prime_UTR 35.0%
ATGCAATGTACATCAACAAC+CGG + chr8_1:89352387-89352406 Msa1224420:three_prime_UTR 35.0%
ATCGTCGAAGACTATTTAAG+AGG + chr8_1:89352466-89352485 Msa1224420:three_prime_UTR 35.0%
AAGCAATAAAGCATGTTACG+GGG - chr8_1:89352590-89352609 None:intergenic 35.0%
! TTTTTGTCTTGGACTCTTCT+AGG + chr8_1:89352682-89352701 Msa1224420:three_prime_UTR 35.0%
AAGAAGTTAGTCACTTCATC+AGG - chr8_1:89352786-89352805 None:intergenic 35.0%
GATGAAGTGACTAACTTCTT+TGG + chr8_1:89352786-89352805 Msa1224420:three_prime_UTR 35.0%
CTTTGACGAAATAATGCATC+AGG + chr8_1:89352853-89352872 Msa1224420:three_prime_UTR 35.0%
CACAACAAGTAAAACATGAC+TGG - chr8_1:89353042-89353061 None:intergenic 35.0%
TGAACTTCTGATGCAATTGT+AGG + chr8_1:89353060-89353079 Msa1224420:three_prime_UTR 35.0%
TAAACGGTGATGATCTTGTG+TGG + chr8_1:89349750-89349769 Msa1224420:CDS 40.0%
AAACGGTGATGATCTTGTGT+GGG + chr8_1:89349751-89349770 Msa1224420:CDS 40.0%
TTCAAAGCCAAGTGTAGTCA+TGG - chr8_1:89349777-89349796 None:intergenic 40.0%
AGAATATGCAGAGCCTCTTA+AGG + chr8_1:89349796-89349815 Msa1224420:CDS 40.0%
GAATATGCAGAGCCTCTTAA+GGG + chr8_1:89349797-89349816 Msa1224420:CDS 40.0%
TTGTGAAGGTAACCCTTAAG+AGG - chr8_1:89349812-89349831 None:intergenic 40.0%
AGGGTTACCTTCACAAGTAT+AGG + chr8_1:89349816-89349835 Msa1224420:CDS 40.0%
GGGTTACCTTCACAAGTATA+GGG + chr8_1:89349817-89349836 Msa1224420:CDS 40.0%
! GTAATCATATGTGTCGGTCT+CGG - chr8_1:89350256-89350275 None:intergenic 40.0%
! TTTCAAGTGTAAGTGCAGGT+AGG - chr8_1:89350471-89350490 None:intergenic 40.0%
! GTTCTTTCAAGTGTAAGTGC+AGG - chr8_1:89350475-89350494 None:intergenic 40.0%
ATACACCCCTTATGTGACAT+GGG + chr8_1:89350662-89350681 Msa1224420:intron 40.0%
!!! GTTTTTTGTAGTGGGTGTGA+TGG - chr8_1:89350826-89350845 None:intergenic 40.0%
! TTTTCAGAGAGAGAGAGAGA+GGG - chr8_1:89350860-89350879 None:intergenic 40.0%
! TCTAGTGAGAATCTAGAGTG+TGG - chr8_1:89351019-89351038 None:intergenic 40.0%
CACTAGAAATCCATCCAGAA+AGG + chr8_1:89351032-89351051 Msa1224420:intron 40.0%
GATTCACTAACCTTTCTGGA+TGG - chr8_1:89351045-89351064 None:intergenic 40.0%
ATTCTCGGCTACTTAAACTC+AGG + chr8_1:89351562-89351581 Msa1224420:intron 40.0%
GTTATATGTAGCTCACTTGG+TGG + chr8_1:89351729-89351748 Msa1224420:intron 40.0%
! TCACTTGGTGGTACTATTAG+TGG + chr8_1:89351741-89351760 Msa1224420:intron 40.0%
! TGCCTTATTAATCAGCTGCA+TGG + chr8_1:89351830-89351849 Msa1224420:intron 40.0%
ATCTCAACCTATCGGGAATT+AGG - chr8_1:89351877-89351896 None:intergenic 40.0%
! TAATTCCCGATAGGTTGAGA+TGG + chr8_1:89351876-89351895 Msa1224420:intron 40.0%
AACTTCCATCTCAACCTATC+GGG - chr8_1:89351884-89351903 None:intergenic 40.0%
CAACTTCCATCTCAACCTAT+CGG - chr8_1:89351885-89351904 None:intergenic 40.0%
TGAGATGGAAGTTGCAGATA+TGG + chr8_1:89351891-89351910 Msa1224420:intron 40.0%
GAGATGGAAGTTGCAGATAT+GGG + chr8_1:89351892-89351911 Msa1224420:intron 40.0%
! GTGATTGCTAGCGCATTTTA+TGG + chr8_1:89352054-89352073 Msa1224420:three_prime_UTR 40.0%
TTAACTGAACGCTTCAACAG+AGG - chr8_1:89352097-89352116 None:intergenic 40.0%
! TGTTGAAGCGTTCAGTTAAC+CGG + chr8_1:89352098-89352117 Msa1224420:three_prime_UTR 40.0%
! TGGAAGTGGGAGTTTTTCTT+GGG + chr8_1:89352134-89352153 Msa1224420:three_prime_UTR 40.0%
!! CACTTGTTTTCTGCTATGCT+TGG + chr8_1:89352223-89352242 Msa1224420:three_prime_UTR 40.0%
TAACAAAAATGTTGCAGGGC+AGG - chr8_1:89352249-89352268 None:intergenic 40.0%
CTGAATGAAACAGCTTCAAC+CGG - chr8_1:89352284-89352303 None:intergenic 40.0%
CCTTGTGTGTTACCTTTGTT+GGG + chr8_1:89352340-89352359 Msa1224420:three_prime_UTR 40.0%
AATATAACTCCTCCGTTCTC+TGG - chr8_1:89352430-89352449 None:intergenic 40.0%
CAAGACAAAAACCTTTGCAG+CGG - chr8_1:89352673-89352692 None:intergenic 40.0%
TAATGCATCAGGTCACTTGT+TGG + chr8_1:89352864-89352883 Msa1224420:three_prime_UTR 40.0%
CAGGAAGTTTGACGGAATCA+TGG - chr8_1:89350440-89350459 None:intergenic 45.0%
TGGAAGAGTGGTATGGAGAA+GGG - chr8_1:89350643-89350662 None:intergenic 45.0%
ATGGAAGAGTGGTATGGAGA+AGG - chr8_1:89350644-89350663 None:intergenic 45.0%
! ATAAGGGGTGTATGGAAGAG+TGG - chr8_1:89350655-89350674 None:intergenic 45.0%
! CATGTCACATAAGGGGTGTA+TGG - chr8_1:89350663-89350682 None:intergenic 45.0%
CATACACCCCTTATGTGACA+TGG + chr8_1:89350661-89350680 Msa1224420:intron 45.0%
!!! TTTTGTAGTGGGTGTGATGG+CGG - chr8_1:89350823-89350842 None:intergenic 45.0%
!!! GATGGCGGGTTTTTTGTAGT+GGG - chr8_1:89350834-89350853 None:intergenic 45.0%
!!! TGATGGCGGGTTTTTTGTAG+TGG - chr8_1:89350835-89350854 None:intergenic 45.0%
! GTTTTCAGAGAGAGAGAGAG+AGG - chr8_1:89350861-89350880 None:intergenic 45.0%
AGAGGGATAAGGGAGATTGA+GGG - chr8_1:89350903-89350922 None:intergenic 45.0%
AGATGAGGTTTCGAGAGACA+GGG - chr8_1:89350926-89350945 None:intergenic 45.0%
CGGTCGCAAATCAAATTCGA+AGG - chr8_1:89351159-89351178 None:intergenic 45.0%
ATTGAGGGACCCTATATGTG+AGG + chr8_1:89351610-89351629 Msa1224420:intron 45.0%
TTGAGGGACCCTATATGTGA+GGG + chr8_1:89351611-89351630 Msa1224420:intron 45.0%
! CTGGAAGTGGGAGTTTTTCT+TGG + chr8_1:89352133-89352152 Msa1224420:three_prime_UTR 45.0%
! GATGTGGCTGCAGTTTTTCT+TGG + chr8_1:89352180-89352199 Msa1224420:three_prime_UTR 45.0%
TCATTCAGTTTAGAGAGGGG+AGG + chr8_1:89352296-89352315 Msa1224420:three_prime_UTR 45.0%
GCCTTGTGTGTTACCTTTGT+TGG + chr8_1:89352339-89352358 Msa1224420:three_prime_UTR 45.0%
CCCAACAAAGGTAACACACA+AGG - chr8_1:89352343-89352362 None:intergenic 45.0%
! TGAGTGCAGACTGTGTTGTT+TGG + chr8_1:89352363-89352382 Msa1224420:three_prime_UTR 45.0%
CAACTGGAAACACCAGAGAA+CGG + chr8_1:89352415-89352434 Msa1224420:three_prime_UTR 45.0%
CTTCAGAACATACTTGCCAC+AGG + chr8_1:89352644-89352663 Msa1224420:intron 45.0%
! CGCTGCAAAGGTTTTTGTCT+TGG + chr8_1:89352671-89352690 Msa1224420:three_prime_UTR 45.0%
! TGTCTTGGACTCTTCTAGGA+TGG + chr8_1:89352686-89352705 Msa1224420:three_prime_UTR 45.0%
GGTAGTAGTAAGGAGCAGCA+AGG + chr8_1:89349878-89349897 Msa1224420:CDS 50.0%
! ATCGGTGTCCGCCTTATGTT+CGG + chr8_1:89350306-89350325 Msa1224420:intron 50.0%
AGACACGGACACCGAACATA+AGG - chr8_1:89350320-89350339 None:intergenic 50.0%
! GTGCAGGTAGGTTGAGTATC+AGG - chr8_1:89350459-89350478 None:intergenic 50.0%
GGGTGTATGGAAGAGTGGTA+TGG - chr8_1:89350650-89350669 None:intergenic 50.0%
TCTCTCTTCTGTCATCCCAG+AGG + chr8_1:89350753-89350772 Msa1224420:intron 50.0%
AGGGAGATTGAGGGTGATGA+AGG - chr8_1:89350894-89350913 None:intergenic 50.0%
GAGAGGGATAAGGGAGATTG+AGG - chr8_1:89350904-89350923 None:intergenic 50.0%
AGAGACAGGGAGAGGGATAA+GGG - chr8_1:89350913-89350932 None:intergenic 50.0%
GAGATGAGGTTTCGAGAGAC+AGG - chr8_1:89350927-89350946 None:intergenic 50.0%
TGAGGTTGACGAAGGTGAGA+GGG - chr8_1:89350949-89350968 None:intergenic 50.0%
TTGAGGTTGACGAAGGTGAG+AGG - chr8_1:89350950-89350969 None:intergenic 50.0%
AGGGAGATTGAGGTTGACGA+AGG - chr8_1:89350957-89350976 None:intergenic 50.0%
AGAAACAAAAGTGGCGGCGA+GGG - chr8_1:89350976-89350995 None:intergenic 50.0%
GGCTACTTAAACTCAGGCTG+TGG + chr8_1:89351568-89351587 Msa1224420:intron 50.0%
AGATTGCCGATGCAGGATTG+AGG + chr8_1:89351594-89351613 Msa1224420:intron 50.0%
GATTGCCGATGCAGGATTGA+GGG + chr8_1:89351595-89351614 Msa1224420:intron 50.0%
CAGGAAAGTCACGAGTCGAT+AGG - chr8_1:89352020-89352039 None:intergenic 50.0%
! GAAGCGTTCAGTTAACCGGT+AGG + chr8_1:89352102-89352121 Msa1224420:three_prime_UTR 50.0%
!! GTGTTGCTGCCTCAGGTTTT+AGG + chr8_1:89352156-89352175 Msa1224420:three_prime_UTR 50.0%
!!! GCCTCAGGTTTTAGGTGATG+TGG + chr8_1:89352164-89352183 Msa1224420:three_prime_UTR 50.0%
GCCACATCACCTAAAACCTG+AGG - chr8_1:89352168-89352187 None:intergenic 50.0%
! GTGGCTGCAGTTTTTCTTGG+TGG + chr8_1:89352183-89352202 Msa1224420:three_prime_UTR 50.0%
! GAGGATGTGTTGCTGTCACA+TGG + chr8_1:89352315-89352334 Msa1224420:three_prime_UTR 50.0%
AGTCTGCACTCACCCAACAA+AGG - chr8_1:89352355-89352374 None:intergenic 50.0%
TCAACAACCGGCTAAGCAAC+TGG + chr8_1:89352399-89352418 Msa1224420:three_prime_UTR 50.0%
GGTGTTTCCAGTTGCTTAGC+CGG - chr8_1:89352409-89352428 None:intergenic 50.0%
GTGTGGGCCATGACTACACT+TGG + chr8_1:89349767-89349786 Msa1224420:CDS 55.0%
CACGGACACCGAACATAAGG+CGG - chr8_1:89350317-89350336 None:intergenic 55.0%
GTGAAGCACGGGTACAGACA+CGG - chr8_1:89350335-89350354 None:intergenic 55.0%
! AGAGAGAGAGGGTGTGATGG+CGG - chr8_1:89350849-89350868 None:intergenic 55.0%
! GAGAGAGAGAGAGGGTGTGA+TGG - chr8_1:89350852-89350871 None:intergenic 55.0%
GGGTGATGAAGGCGAGATTG+AGG - chr8_1:89350883-89350902 None:intergenic 55.0%
GAGAGACAGGGAGAGGGATA+AGG - chr8_1:89350914-89350933 None:intergenic 55.0%
GGTTTCGAGAGACAGGGAGA+GGG - chr8_1:89350920-89350939 None:intergenic 55.0%
AGGTTTCGAGAGACAGGGAG+AGG - chr8_1:89350921-89350940 None:intergenic 55.0%
ACGAAGGTGAGAGGGAGATG+AGG - chr8_1:89350941-89350960 None:intergenic 55.0%
GAGAAACAAAAGTGGCGGCG+AGG - chr8_1:89350977-89350996 None:intergenic 55.0%
GTGGAAGAGATTGCCGATGC+AGG + chr8_1:89351587-89351606 Msa1224420:intron 55.0%
AGGGTCCCTCAATCCTGCAT+CGG - chr8_1:89351603-89351622 None:intergenic 55.0%
TTGGGCGCCTAATTCCCGAT+AGG + chr8_1:89351867-89351886 Msa1224420:intron 55.0%
TAGGTCGCGTGACTGGAAGT+GGG + chr8_1:89352121-89352140 Msa1224420:three_prime_UTR 55.0%
!! TTCTTGGGTGTTGCTGCCTC+AGG + chr8_1:89352149-89352168 Msa1224420:three_prime_UTR 55.0%
CTGGAAACACCAGAGAACGG+AGG + chr8_1:89352418-89352437 Msa1224420:three_prime_UTR 55.0%
ACCTTTGCAGCGGAAACCTG+TGG - chr8_1:89352663-89352682 None:intergenic 55.0%
!! TGGTGTTGATGGCGGAGGCT+TGG - chr8_1:89350783-89350802 None:intergenic 60.0%
!! GGTGGCGTTGGTGTTGATGG+CGG - chr8_1:89350791-89350810 None:intergenic 60.0%
!! TGTAGTGGGTGTGATGGCGG+AGG - chr8_1:89350820-89350839 None:intergenic 60.0%
! GAGAGAGAGGGTGTGATGGC+GGG - chr8_1:89350848-89350867 None:intergenic 60.0%
TAACCGGTAGGTCGCGTGAC+TGG + chr8_1:89352114-89352133 Msa1224420:three_prime_UTR 60.0%
CTTCCAGTCACGCGACCTAC+CGG - chr8_1:89352120-89352139 None:intergenic 60.0%
GTAGGTCGCGTGACTGGAAG+TGG + chr8_1:89352120-89352139 Msa1224420:three_prime_UTR 60.0%
GCCACAGGTTTCCGCTGCAA+AGG + chr8_1:89352659-89352678 Msa1224420:intron 60.0%
! CGGAGGCTTGGTCTGCCTCT+GGG - chr8_1:89350771-89350790 None:intergenic 65.0%
!! GGCGTTGGTGTTGATGGCGG+AGG - chr8_1:89350788-89350807 None:intergenic 65.0%
!! GGCGGTGGCGTTGGTGTTGA+TGG - chr8_1:89350794-89350813 None:intergenic 65.0%
AGTGGCGGCGAGGGAGATTG+AGG - chr8_1:89350967-89350986 None:intergenic 65.0%
! GCGGAGGCTTGGTCTGCCTC+TGG - chr8_1:89350772-89350791 None:intergenic 70.0%
!! TGGGTGTGATGGCGGAGGCG+CGG - chr8_1:89350815-89350834 None:intergenic 70.0%
!! GTGTGATGGCGGAGGCGCGG+CGG - chr8_1:89350812-89350831 None:intergenic 75.0%
!!! TGATGGCGGAGGCGCGGCGG+TGG - chr8_1:89350809-89350828 None:intergenic 80.0%
!! CGGAGGCGCGGCGGTGGCGT+TGG - chr8_1:89350803-89350822 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 89349668 89353126 89349668 ID=Msa1224420;Name=Msa1224420
chr8_1 mRNA 89349668 89353126 89349668 ID=Msa1224420-mRNA-1;Parent=Msa1224420;Name=Msa1224420-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|0.5|0.66|1|0.5|0.33|3|932|80
chr8_1 exon 89349668 89349899 89349668 ID=Msa1224420-mRNA-1:exon:32410;Parent=Msa1224420-mRNA-1
chr8_1 exon 89352006 89352487 89352006 ID=Msa1224420-mRNA-1:exon:32411;Parent=Msa1224420-mRNA-1
chr8_1 exon 89352666 89353126 89352666 ID=Msa1224420-mRNA-1:exon:32412;Parent=Msa1224420-mRNA-1
chr8_1 CDS 89349668 89349899 89349668 ID=Msa1224420-mRNA-1:cds;Parent=Msa1224420-mRNA-1
chr8_1 CDS 89352006 89352016 89352006 ID=Msa1224420-mRNA-1:cds;Parent=Msa1224420-mRNA-1
chr8_1 three_prime_UTR 89352017 89352487 89352017 ID=Msa1224420-mRNA-1:three_prime_utr;Parent=Msa1224420-mRNA-1
chr8_1 three_prime_UTR 89352666 89353126 89352666 ID=Msa1224420-mRNA-1:three_prime_utr;Parent=Msa1224420-mRNA-1
Gene Sequence

>Msa1224420

ATGCAAGAGTGTGTATCAGAGTTCATAAGCTTCATAACAGGTGAAGCATCTGAAAAGTGTCAGAAAGAGAAGAGGAAGACAATAAACGGTGATGATCTTGTGTGGGCCATGACTACACTTGGCTTTGAAGAATATGCAGAGCCTCTTAAGGGTTACCTTCACAAGTATAGGGAAATTGAAGGTGACAAGAATTTTTCTATGAATATGATTGGTAGTAGTAAGGAGCAGCAAGGTACAATGTAG
Protein sequence

>Msa1224420

MQECVSEFISFITGEASEKCQKEKRKTINGDDLVWAMTTLGFEEYAEPLKGYLHKYREIEGDKNFSMNMIGSSKEQQGTM*