Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1226150 | XP_003620925.1 | 89.759 | 166 | 17 | 0 | 2 | 167 | 138 | 303 | 7.44e-103 | 310 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1226150 | sp|F4K933|ET2_ARATH | 40.000 | 155 | 89 | 2 | 2 | 152 | 153 | 307 | 1.98e-26 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa1226150 | TF | HRT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1226150 | MtrunA17_Chr6g0488451 | 89.759 | 166 | 17 | 0 | 2 | 167 | 138 | 303 | 6.84e-107 | 310 |
| Msa1226150 | MtrunA17_Chr7g0256351 | 70.186 | 161 | 47 | 1 | 2 | 161 | 142 | 302 | 7.97e-74 | 226 |
| Msa1226150 | MtrunA17_Chr6g0488421 | 37.864 | 103 | 49 | 2 | 2 | 90 | 39 | 140 | 3.14e-14 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1226150 | AT5G56780.1 | 40.000 | 155 | 89 | 2 | 2 | 152 | 153 | 307 | 2.01e-27 | 107 |
Find 28 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAACATACTTGATTTAAT+TGG | 0.224850 | 8_1:+91926524 | None:intergenic |
| TAAAATTTGTGGAGTGAATT+TGG | 0.231337 | 8_1:-91926372 | Msa1226150:CDS |
| GTTGTATCTTGAACTATCTT+AGG | 0.344160 | 8_1:+91926419 | None:intergenic |
| AGTGAAGGGTAGAAGTGATT+TGG | 0.349328 | 8_1:+91926565 | None:intergenic |
| TCGGATTTGTTATCTTTCAT+AGG | 0.354876 | 8_1:+91926503 | None:intergenic |
| GGAATTTGAACACGCGAGAT+AGG | 0.386624 | 8_1:+91926453 | None:intergenic |
| TGTTTGCAAGGCATCTCCCT+TGG | 0.436980 | 8_1:+91926715 | None:intergenic |
| GAGAAGAGTGGTAAAATTTG+TGG | 0.450994 | 8_1:-91926383 | Msa1226150:CDS |
| GCTGCCATTGTCTGCTTCAT+CGG | 0.453379 | 8_1:+91926484 | None:intergenic |
| ATCTGAAAATGTTCTTGTGC+TGG | 0.457395 | 8_1:+91926592 | None:intergenic |
| GTTAGGTGTCATGAACATAA+AGG | 0.469142 | 8_1:-91926308 | Msa1226150:CDS |
| TGAGTACATTTGATTATGCT+TGG | 0.471865 | 8_1:-91926679 | Msa1226150:CDS |
| GCTTGGAACACAATTAATAA+CGG | 0.491690 | 8_1:-91926662 | Msa1226150:CDS |
| CAGTTGAAATTCTGTTTGCA+AGG | 0.502089 | 8_1:+91926703 | None:intergenic |
| CCTTCACTCAAAAGAAAGTA+GGG | 0.503569 | 8_1:-91926550 | Msa1226150:CDS |
| CCCTTCACTCAAAAGAAAGT+AGG | 0.510670 | 8_1:-91926551 | Msa1226150:CDS |
| GAGCATTTCAAGAATATCAC+CGG | 0.525607 | 8_1:+91926628 | None:intergenic |
| CTATCTTAGGACGCGACCGG+TGG | 0.570672 | 8_1:+91926432 | None:intergenic |
| AATAACGGTCCACGGCGTCC+CGG | 0.572061 | 8_1:-91926647 | Msa1226150:CDS |
| CTCGCGTGTTCAAATTCCAC+CGG | 0.598427 | 8_1:-91926448 | Msa1226150:CDS |
| GAACTATCTTAGGACGCGAC+CGG | 0.598468 | 8_1:+91926429 | None:intergenic |
| TTCTATTTGTACAAAGACGC+CGG | 0.623541 | 8_1:-91926342 | Msa1226150:CDS |
| AGAATATCACCGGGACGCCG+TGG | 0.625598 | 8_1:+91926638 | None:intergenic |
| GTTGAACAGATGCAAACCAA+GGG | 0.638402 | 8_1:-91926731 | Msa1226150:intron |
| ACACAATTAATAACGGTCCA+CGG | 0.642408 | 8_1:-91926655 | Msa1226150:CDS |
| TGTTGAACAGATGCAAACCA+AGG | 0.647561 | 8_1:-91926732 | Msa1226150:intron |
| AGCATTTCAAGAATATCACC+GGG | 0.651967 | 8_1:+91926629 | None:intergenic |
| AAATCCGATGAAGCAGACAA+TGG | 0.675612 | 8_1:-91926488 | Msa1226150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTAGAAGCTTTAATAAT+TGG | - | chr8_1:91926271-91926290 | Msa1226150:CDS | 15.0% |
| !! | AATCTAATAGAATAAACAAT+TGG | - | chr8_1:91926450-91926469 | Msa1226150:CDS | 15.0% |
| !!! | GCATTTATATTTAATCTTTT+GGG | + | chr8_1:91926966-91926985 | None:intergenic | 15.0% |
| !!! | TGCATTTATATTTAATCTTT+TGG | + | chr8_1:91926967-91926986 | None:intergenic | 15.0% |
| !!! | ATATTAGAAGCTTTAATAAT+TGG | - | chr8_1:91926271-91926290 | Msa1226150:CDS | 15.0% |
| !! | AATCTAATAGAATAAACAAT+TGG | - | chr8_1:91926450-91926469 | Msa1226150:CDS | 15.0% |
| !!! | GCATTTATATTTAATCTTTT+GGG | + | chr8_1:91926966-91926985 | None:intergenic | 15.0% |
| !!! | TGCATTTATATTTAATCTTT+TGG | + | chr8_1:91926967-91926986 | None:intergenic | 15.0% |
| !! | TTAACAAGAAAAGTAAACAA+CGG | - | chr8_1:91926299-91926318 | Msa1226150:CDS | 20.0% |
| !! | TGTTTATTCTATTAGATTAG+AGG | + | chr8_1:91926448-91926467 | None:intergenic | 20.0% |
| !!! | TTTTCCAATTGTTTATAGAT+GGG | - | chr8_1:91926537-91926556 | Msa1226150:CDS | 20.0% |
| !! | CAAAATGAACATATACAATA+TGG | + | chr8_1:91926679-91926698 | None:intergenic | 20.0% |
| !! | ATAATATGTATCTCAAATCA+TGG | + | chr8_1:91926708-91926727 | None:intergenic | 20.0% |
| !! | TTAATTAAGAGCTAAGTTAT+AGG | - | chr8_1:91926788-91926807 | Msa1226150:intron | 20.0% |
| !! | TTAACAAGAAAAGTAAACAA+CGG | - | chr8_1:91926299-91926318 | Msa1226150:CDS | 20.0% |
| !! | TGTTTATTCTATTAGATTAG+AGG | + | chr8_1:91926448-91926467 | None:intergenic | 20.0% |
| !!! | TTTTCCAATTGTTTATAGAT+GGG | - | chr8_1:91926537-91926556 | Msa1226150:CDS | 20.0% |
| !! | CAAAATGAACATATACAATA+TGG | + | chr8_1:91926679-91926698 | None:intergenic | 20.0% |
| !! | ATAATATGTATCTCAAATCA+TGG | + | chr8_1:91926708-91926727 | None:intergenic | 20.0% |
| !! | TTAATTAAGAGCTAAGTTAT+AGG | - | chr8_1:91926788-91926807 | Msa1226150:intron | 20.0% |
| ! | AAATTAAGAGACATGATTCA+AGG | - | chr8_1:91926514-91926533 | Msa1226150:CDS | 25.0% |
| !! | GTTTTCCAATTGTTTATAGA+TGG | - | chr8_1:91926536-91926555 | Msa1226150:CDS | 25.0% |
| ! | TCAACTTATTAAGATCAAGA+TGG | - | chr8_1:91926599-91926618 | Msa1226150:CDS | 25.0% |
| !! | TATGAAGTAAGTATTTTCTC+TGG | - | chr8_1:91926843-91926862 | Msa1226150:intron | 25.0% |
| ! | TAAAATTTGTGGAGTGAATT+TGG | - | chr8_1:91927408-91927427 | Msa1226150:intron | 25.0% |
| ! | AAATTAAGAGACATGATTCA+AGG | - | chr8_1:91926514-91926533 | Msa1226150:CDS | 25.0% |
| !! | GTTTTCCAATTGTTTATAGA+TGG | - | chr8_1:91926536-91926555 | Msa1226150:CDS | 25.0% |
| ! | TCAACTTATTAAGATCAAGA+TGG | - | chr8_1:91926599-91926618 | Msa1226150:CDS | 25.0% |
| !! | TATGAAGTAAGTATTTTCTC+TGG | - | chr8_1:91926843-91926862 | Msa1226150:intron | 25.0% |
| ! | TAAAATTTGTGGAGTGAATT+TGG | - | chr8_1:91927408-91927427 | Msa1226150:intron | 25.0% |
| !! | ATGATTCTAGCTTCTTTCAT+TGG | - | chr8_1:91926401-91926420 | Msa1226150:CDS | 30.0% |
| GTATTAAGAGACATGATTCA+AGG | + | chr8_1:91926480-91926499 | None:intergenic | 30.0% | |
| AATGCATAGAATGTACTTAC+AGG | + | chr8_1:91926565-91926584 | None:intergenic | 30.0% | |
| GAACATATACAATATGGTCA+AGG | + | chr8_1:91926673-91926692 | None:intergenic | 30.0% | |
| TAAGAGCTAAGTTATAGGTT+TGG | - | chr8_1:91926793-91926812 | Msa1226150:intron | 30.0% | |
| CACATTTGCATCTTACATAA+AGG | - | chr8_1:91926985-91927004 | Msa1226150:intron | 30.0% | |
| TGAGTACATTTGATTATGCT+TGG | - | chr8_1:91927101-91927120 | Msa1226150:intron | 30.0% | |
| GCTTGGAACACAATTAATAA+CGG | - | chr8_1:91927118-91927137 | Msa1226150:intron | 30.0% | |
| GGCAACATACTTGATTTAAT+TGG | + | chr8_1:91927259-91927278 | None:intergenic | 30.0% | |
| !! | TCGGATTTGTTATCTTTCAT+AGG | + | chr8_1:91927280-91927299 | None:intergenic | 30.0% |
| GTTGTATCTTGAACTATCTT+AGG | + | chr8_1:91927364-91927383 | None:intergenic | 30.0% | |
| ! | AATTTTACCACTCTTCTCAA+CGG | + | chr8_1:91927395-91927414 | None:intergenic | 30.0% |
| !! | ATGATTCTAGCTTCTTTCAT+TGG | - | chr8_1:91926401-91926420 | Msa1226150:CDS | 30.0% |
| GTATTAAGAGACATGATTCA+AGG | + | chr8_1:91926480-91926499 | None:intergenic | 30.0% | |
| AATGCATAGAATGTACTTAC+AGG | + | chr8_1:91926565-91926584 | None:intergenic | 30.0% | |
| GAACATATACAATATGGTCA+AGG | + | chr8_1:91926673-91926692 | None:intergenic | 30.0% | |
| TAAGAGCTAAGTTATAGGTT+TGG | - | chr8_1:91926793-91926812 | Msa1226150:intron | 30.0% | |
| CACATTTGCATCTTACATAA+AGG | - | chr8_1:91926985-91927004 | Msa1226150:intron | 30.0% | |
| TGAGTACATTTGATTATGCT+TGG | - | chr8_1:91927101-91927120 | Msa1226150:intron | 30.0% | |
| GCTTGGAACACAATTAATAA+CGG | - | chr8_1:91927118-91927137 | Msa1226150:intron | 30.0% | |
| GGCAACATACTTGATTTAAT+TGG | + | chr8_1:91927259-91927278 | None:intergenic | 30.0% | |
| !! | TCGGATTTGTTATCTTTCAT+AGG | + | chr8_1:91927280-91927299 | None:intergenic | 30.0% |
| GTTGTATCTTGAACTATCTT+AGG | + | chr8_1:91927364-91927383 | None:intergenic | 30.0% | |
| ! | AATTTTACCACTCTTCTCAA+CGG | + | chr8_1:91927395-91927414 | None:intergenic | 30.0% |
| !! | TTCTAGCTTCTTTCATTGGA+AGG | - | chr8_1:91926405-91926424 | Msa1226150:CDS | 35.0% |
| ATACAATATGGTCAAGGCAA+TGG | + | chr8_1:91926667-91926686 | None:intergenic | 35.0% | |
| AGTTATAGGTTTGGAAGTCT+AGG | - | chr8_1:91926802-91926821 | Msa1226150:intron | 35.0% | |
| CAGTTGAAATTCTGTTTGCA+AGG | + | chr8_1:91927080-91927099 | None:intergenic | 35.0% | |
| ACACAATTAATAACGGTCCA+CGG | - | chr8_1:91927125-91927144 | Msa1226150:intron | 35.0% | |
| AGCATTTCAAGAATATCACC+GGG | + | chr8_1:91927154-91927173 | None:intergenic | 35.0% | |
| GAGCATTTCAAGAATATCAC+CGG | + | chr8_1:91927155-91927174 | None:intergenic | 35.0% | |
| ATCTGAAAATGTTCTTGTGC+TGG | + | chr8_1:91927191-91927210 | None:intergenic | 35.0% | |
| !! | CCTACTTTCTTTTGAGTGAA+GGG | + | chr8_1:91927232-91927251 | None:intergenic | 35.0% |
| CCTTCACTCAAAAGAAAGTA+GGG | - | chr8_1:91927230-91927249 | Msa1226150:intron | 35.0% | |
| GAGAAGAGTGGTAAAATTTG+TGG | - | chr8_1:91927397-91927416 | Msa1226150:intron | 35.0% | |
| TTCTATTTGTACAAAGACGC+CGG | - | chr8_1:91927438-91927457 | Msa1226150:intron | 35.0% | |
| ! | GTTAGGTGTCATGAACATAA+AGG | - | chr8_1:91927472-91927491 | Msa1226150:intron | 35.0% |
| TCCAAGTCAGAATCAAAAAG+AGG | - | chr8_1:91927511-91927530 | Msa1226150:intron | 35.0% | |
| !! | TCCTCTTTTTGATTCTGACT+TGG | + | chr8_1:91927515-91927534 | None:intergenic | 35.0% |
| CAAAAAGAGGAAATGCTTGT+AGG | - | chr8_1:91927524-91927543 | Msa1226150:intron | 35.0% | |
| !! | TTCTAGCTTCTTTCATTGGA+AGG | - | chr8_1:91926405-91926424 | Msa1226150:CDS | 35.0% |
| ATACAATATGGTCAAGGCAA+TGG | + | chr8_1:91926667-91926686 | None:intergenic | 35.0% | |
| AGTTATAGGTTTGGAAGTCT+AGG | - | chr8_1:91926802-91926821 | Msa1226150:intron | 35.0% | |
| CAGTTGAAATTCTGTTTGCA+AGG | + | chr8_1:91927080-91927099 | None:intergenic | 35.0% | |
| ACACAATTAATAACGGTCCA+CGG | - | chr8_1:91927125-91927144 | Msa1226150:intron | 35.0% | |
| AGCATTTCAAGAATATCACC+GGG | + | chr8_1:91927154-91927173 | None:intergenic | 35.0% | |
| GAGCATTTCAAGAATATCAC+CGG | + | chr8_1:91927155-91927174 | None:intergenic | 35.0% | |
| ATCTGAAAATGTTCTTGTGC+TGG | + | chr8_1:91927191-91927210 | None:intergenic | 35.0% | |
| !! | CCTACTTTCTTTTGAGTGAA+GGG | + | chr8_1:91927232-91927251 | None:intergenic | 35.0% |
| CCTTCACTCAAAAGAAAGTA+GGG | - | chr8_1:91927230-91927249 | Msa1226150:intron | 35.0% | |
| GAGAAGAGTGGTAAAATTTG+TGG | - | chr8_1:91927397-91927416 | Msa1226150:intron | 35.0% | |
| TTCTATTTGTACAAAGACGC+CGG | - | chr8_1:91927438-91927457 | Msa1226150:intron | 35.0% | |
| ! | GTTAGGTGTCATGAACATAA+AGG | - | chr8_1:91927472-91927491 | Msa1226150:intron | 35.0% |
| TCCAAGTCAGAATCAAAAAG+AGG | - | chr8_1:91927511-91927530 | Msa1226150:intron | 35.0% | |
| !! | TCCTCTTTTTGATTCTGACT+TGG | + | chr8_1:91927515-91927534 | None:intergenic | 35.0% |
| CAAAAAGAGGAAATGCTTGT+AGG | - | chr8_1:91927524-91927543 | Msa1226150:intron | 35.0% | |
| AACATTTGTGTCGTGTCTCA+TGG | + | chr8_1:91926352-91926371 | None:intergenic | 40.0% | |
| GGAGCCCATCTATAAACAAT+TGG | + | chr8_1:91926544-91926563 | None:intergenic | 40.0% | |
| TGTTGAACAGATGCAAACCA+AGG | - | chr8_1:91927048-91927067 | Msa1226150:intron | 40.0% | |
| GTTGAACAGATGCAAACCAA+GGG | - | chr8_1:91927049-91927068 | Msa1226150:intron | 40.0% | |
| AGTGAAGGGTAGAAGTGATT+TGG | + | chr8_1:91927218-91927237 | None:intergenic | 40.0% | |
| CCCTTCACTCAAAAGAAAGT+AGG | - | chr8_1:91927229-91927248 | Msa1226150:intron | 40.0% | |
| !! | CCCTACTTTCTTTTGAGTGA+AGG | + | chr8_1:91927233-91927252 | None:intergenic | 40.0% |
| AAATCCGATGAAGCAGACAA+TGG | - | chr8_1:91927292-91927311 | Msa1226150:intron | 40.0% | |
| ! | GATTTTTCCGTTGAGAAGAG+TGG | - | chr8_1:91927385-91927404 | Msa1226150:intron | 40.0% |
| !!! | CACCTAACTCTTTTTTCGAC+CGG | + | chr8_1:91927460-91927479 | None:intergenic | 40.0% |
| AACATTTGTGTCGTGTCTCA+TGG | + | chr8_1:91926352-91926371 | None:intergenic | 40.0% | |
| GGAGCCCATCTATAAACAAT+TGG | + | chr8_1:91926544-91926563 | None:intergenic | 40.0% | |
| TGTTGAACAGATGCAAACCA+AGG | - | chr8_1:91927048-91927067 | Msa1226150:intron | 40.0% | |
| GTTGAACAGATGCAAACCAA+GGG | - | chr8_1:91927049-91927068 | Msa1226150:intron | 40.0% | |
| AGTGAAGGGTAGAAGTGATT+TGG | + | chr8_1:91927218-91927237 | None:intergenic | 40.0% | |
| CCCTTCACTCAAAAGAAAGT+AGG | - | chr8_1:91927229-91927248 | Msa1226150:intron | 40.0% | |
| !! | CCCTACTTTCTTTTGAGTGA+AGG | + | chr8_1:91927233-91927252 | None:intergenic | 40.0% |
| AAATCCGATGAAGCAGACAA+TGG | - | chr8_1:91927292-91927311 | Msa1226150:intron | 40.0% | |
| ! | GATTTTTCCGTTGAGAAGAG+TGG | - | chr8_1:91927385-91927404 | Msa1226150:intron | 40.0% |
| !!! | CACCTAACTCTTTTTTCGAC+CGG | + | chr8_1:91927460-91927479 | None:intergenic | 40.0% |
| GGAATTTGAACACGCGAGAT+AGG | + | chr8_1:91927330-91927349 | None:intergenic | 45.0% | |
| GGAATTTGAACACGCGAGAT+AGG | + | chr8_1:91927330-91927349 | None:intergenic | 45.0% | |
| ! | TGTTTGCAAGGCATCTCCCT+TGG | + | chr8_1:91927068-91927087 | None:intergenic | 50.0% |
| GCTGCCATTGTCTGCTTCAT+CGG | + | chr8_1:91927299-91927318 | None:intergenic | 50.0% | |
| CTCGCGTGTTCAAATTCCAC+CGG | - | chr8_1:91927332-91927351 | Msa1226150:intron | 50.0% | |
| GAACTATCTTAGGACGCGAC+CGG | + | chr8_1:91927354-91927373 | None:intergenic | 50.0% | |
| CGCCGGTCGAAAAAAGAGTT+AGG | - | chr8_1:91927455-91927474 | Msa1226150:intron | 50.0% | |
| ! | TGTTTGCAAGGCATCTCCCT+TGG | + | chr8_1:91927068-91927087 | None:intergenic | 50.0% |
| GCTGCCATTGTCTGCTTCAT+CGG | + | chr8_1:91927299-91927318 | None:intergenic | 50.0% | |
| CTCGCGTGTTCAAATTCCAC+CGG | - | chr8_1:91927332-91927351 | Msa1226150:intron | 50.0% | |
| GAACTATCTTAGGACGCGAC+CGG | + | chr8_1:91927354-91927373 | None:intergenic | 50.0% | |
| CGCCGGTCGAAAAAAGAGTT+AGG | - | chr8_1:91927455-91927474 | Msa1226150:intron | 50.0% | |
| AATAACGGTCCACGGCGTCC+CGG | - | chr8_1:91927133-91927152 | Msa1226150:intron | 60.0% | |
| AGAATATCACCGGGACGCCG+TGG | + | chr8_1:91927145-91927164 | None:intergenic | 60.0% | |
| CTATCTTAGGACGCGACCGG+TGG | + | chr8_1:91927351-91927370 | None:intergenic | 60.0% | |
| AATAACGGTCCACGGCGTCC+CGG | - | chr8_1:91927133-91927152 | Msa1226150:intron | 60.0% | |
| AGAATATCACCGGGACGCCG+TGG | + | chr8_1:91927145-91927164 | None:intergenic | 60.0% | |
| CTATCTTAGGACGCGACCGG+TGG | + | chr8_1:91927351-91927370 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 91926250 | 91927552 | 91926250 | ID=Msa1226150;Name=Msa1226150 |
| chr8_1 | mRNA | 91926250 | 91927552 | 91926250 | ID=Msa1226150-mRNA-1;Parent=Msa1226150;Name=Msa1226150-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0|0|0.5|1|1|2|0|167 |
| chr8_1 | exon | 91927544 | 91927552 | 91927544 | ID=Msa1226150-mRNA-1:exon:33501;Parent=Msa1226150-mRNA-1 |
| chr8_1 | exon | 91926250 | 91926744 | 91926250 | ID=Msa1226150-mRNA-1:exon:33500;Parent=Msa1226150-mRNA-1 |
| chr8_1 | CDS | 91927544 | 91927552 | 91927544 | ID=Msa1226150-mRNA-1:cds;Parent=Msa1226150-mRNA-1 |
| chr8_1 | CDS | 91926250 | 91926744 | 91926250 | ID=Msa1226150-mRNA-1:cds;Parent=Msa1226150-mRNA-1 |
| Gene Sequence |
| Protein sequence |