AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1235720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1235720 MtrunA17_Chr8g0345701 70.000 160 35 2 1 148 1 159 3.47e-75 221
Msa1235720 MtrunA17_Chr8g0345671 57.241 145 55 5 7 147 11 152 3.78e-49 162
Msa1235720 MtrunA17_Chr8g0345691 54.861 144 60 4 7 147 12 153 1.83e-48 160
Msa1235720 MtrunA17_Chr8g0345711 49.286 140 65 5 6 141 11 148 1.54e-41 136
Msa1235720 MtrunA17_Chr8g0345741 51.538 130 58 4 18 144 2 129 3.52e-38 127
Msa1235720 MtrunA17_Chr8g0345721 44.375 160 56 4 9 137 37 194 1.10e-37 127
Msa1235720 MtrunA17_Chr8g0345731 44.295 149 73 5 7 147 24 170 1.59e-36 123
Msa1235720 MtrunA17_Chr5g0428991 45.255 137 67 4 7 140 25 156 5.24e-33 122
Msa1235720 MtrunA17_Chr5g0433781 45.522 134 65 4 5 135 4 132 3.68e-31 117
Msa1235720 MtrunA17_Chr3g0128111 44.697 132 65 5 10 138 45 171 1.03e-30 110
Msa1235720 MtrunA17_Chr1g0197361 44.526 137 69 4 2 135 3 135 1.57e-30 115
Msa1235720 MtrunA17_Chr4g0054791 44.526 137 69 4 5 137 4 137 6.93e-30 110
Msa1235720 MtrunA17_Chr5g0400461 43.165 139 68 5 5 135 4 139 7.33e-30 110
Msa1235720 MtrunA17_Chr3g0128151 43.382 136 69 4 6 138 21 151 7.78e-30 113
Msa1235720 MtrunA17_Chr2g0316971 44.776 134 66 5 5 135 21 149 1.05e-29 110
Msa1235720 MtrunA17_Chr3g0130101 44.526 137 67 5 2 135 3 133 1.10e-29 110
Msa1235720 MtrunA17_Chr6g0481091 42.759 145 72 5 3 144 2 138 1.35e-29 107
Msa1235720 MtrunA17_Chr2g0309891 43.609 133 67 4 6 135 5 132 1.82e-29 109
Msa1235720 MtrunA17_Chr3g0109821 44.776 134 66 4 5 135 4 132 1.98e-29 112
Msa1235720 MtrunA17_Chr1g0147421 41.912 136 72 4 2 134 22 153 8.55e-29 108
Msa1235720 MtrunA17_Chr3g0139331 43.836 146 66 6 6 142 10 148 1.69e-28 107
Msa1235720 MtrunA17_Chr8g0384201 42.969 128 65 4 10 134 9 131 2.23e-28 108
Msa1235720 MtrunA17_Chr6g0484441 43.182 132 65 5 6 134 4 128 2.28e-28 106
Msa1235720 MtrunA17_Chr3g0092671 42.636 129 67 3 10 135 8 132 3.30e-28 108
Msa1235720 MtrunA17_Chr1g0183741 39.860 143 74 5 2 139 5 140 5.08e-28 104
Msa1235720 MtrunA17_Chr3g0124761 42.754 138 68 5 1 135 1 130 1.70e-27 103
Msa1235720 MtrunA17_Chr4g0060931 44.697 132 63 6 7 135 17 141 2.02e-27 104
Msa1235720 MtrunA17_Chr1g0201021 41.912 136 70 5 2 134 15 144 2.39e-27 106
Msa1235720 MtrunA17_Chr8g0364791 41.085 129 68 4 10 135 9 132 2.41e-27 105
Msa1235720 MtrunA17_Chr7g0261411 39.860 143 74 5 2 139 5 140 3.48e-27 102
Msa1235720 MtrunA17_Chr2g0318101 42.857 140 70 5 6 142 14 146 4.07e-27 103
Msa1235720 MtrunA17_Chr4g0042561 43.885 139 66 6 1 134 1 132 4.31e-27 102
Msa1235720 MtrunA17_Chr6g0453451 42.424 132 66 5 7 135 20 144 5.18e-27 102
Msa1235720 MtrunA17_Chr3g0091921 44.275 131 63 5 7 134 16 139 7.41e-27 102
Msa1235720 MtrunA17_Chr6g0461661 40.152 132 71 4 7 135 18 144 1.17e-26 102
Msa1235720 MtrunA17_Chr4g0018391 39.855 138 74 5 1 134 1 133 3.04e-26 101
Msa1235720 MtrunA17_Chr4g0047841 43.511 131 66 4 7 134 15 140 4.34e-26 100
Msa1235720 MtrunA17_Chr7g0217711 41.259 143 65 7 10 140 22 157 5.06e-26 97.1
Msa1235720 MtrunA17_Chr7g0253011 39.568 139 74 5 2 135 12 145 6.47e-26 100
Msa1235720 MtrunA17_Chr8g0385991 38.806 134 72 4 5 135 12 138 8.83e-26 97.8
Msa1235720 MtrunA17_Chr7g0217721 41.958 143 64 7 10 140 22 157 8.88e-26 100
Msa1235720 MtrunA17_Chr1g0200561 39.855 138 74 5 1 134 13 145 9.63e-26 100
Msa1235720 MtrunA17_Chr3g0130731 42.029 138 69 5 1 135 1 130 1.05e-25 99.0
Msa1235720 MtrunA17_Chr5g0398971 40.580 138 74 5 1 134 1 134 1.15e-25 99.8
Msa1235720 MtrunA17_Chr1g0197351 43.182 132 67 4 6 134 41 167 1.38e-25 100
Msa1235720 MtrunA17_Chr7g0214071 41.791 134 67 4 7 137 15 140 2.49e-25 97.1
Msa1235720 MtrunA17_Chr3g0144271 37.838 148 81 5 7 147 23 166 2.77e-25 99.8
Msa1235720 MtrunA17_Chr4g0056541 39.855 138 74 5 1 134 1 133 3.77e-25 98.6
Msa1235720 MtrunA17_Chr7g0252971 41.727 139 66 5 7 135 8 141 1.85e-24 96.3
Msa1235720 MtrunA17_Chr7g0263931 41.667 132 69 5 7 135 11 137 2.25e-24 95.9
Msa1235720 MtrunA17_Chr2g0312321 39.130 138 72 5 5 139 9 137 2.34e-24 95.5
Msa1235720 MtrunA17_Chr8g0384721 41.606 137 69 5 1 134 1 129 3.09e-24 94.7
Msa1235720 MtrunA17_Chr5g0417791 41.667 132 67 5 7 135 3 127 1.62e-23 92.4
Msa1235720 MtrunA17_Chr2g0326361 39.098 133 73 4 6 135 13 140 3.56e-23 92.8
Msa1235720 MtrunA17_Chr5g0405211 37.037 135 76 5 5 135 6 135 3.91e-23 92.8
Msa1235720 MtrunA17_Chr8g0372121 37.313 134 75 5 5 134 4 132 4.14e-23 92.4
Msa1235720 MtrunA17_Chr4g0026141 35.862 145 85 4 7 146 5 146 4.21e-23 91.3
Msa1235720 MtrunA17_Chr8g0359921 42.105 133 67 5 6 135 13 138 1.07e-22 91.7
Msa1235720 MtrunA17_Chr2g0318081 42.105 133 67 5 6 135 15 140 1.45e-22 91.3
Msa1235720 MtrunA17_Chr4g0076731 41.912 136 69 6 2 134 50 178 2.97e-22 91.3
Msa1235720 MtrunA17_Chr2g0309331 38.571 140 78 5 6 141 9 144 4.47e-22 90.1
Msa1235720 MtrunA17_Chr4g0038751 38.235 136 73 6 10 141 18 146 4.56e-22 89.4
Msa1235720 MtrunA17_Chr7g0266701 37.500 136 76 5 7 136 4 136 5.39e-22 88.6
Msa1235720 MtrunA17_Chr2g0321741 44.800 125 58 6 28 147 13 131 8.23e-22 89.4
Msa1235720 MtrunA17_Chr4g0017921 37.956 137 76 5 3 135 4 135 1.41e-21 89.0
Msa1235720 MtrunA17_Chr8g0346001 36.567 134 76 5 5 134 13 141 4.02e-21 88.2
Msa1235720 MtrunA17_Chr2g0321631 36.296 135 77 5 8 136 5 136 5.98e-21 85.5
Msa1235720 MtrunA17_Chr2g0311661 36.154 130 74 5 10 134 24 149 3.09e-20 84.3
Msa1235720 MtrunA17_Chr3g0131911 34.459 148 85 5 5 147 12 152 3.39e-20 83.2
Msa1235720 MtrunA17_Chr8g0361731 35.659 129 75 4 10 135 17 140 4.90e-20 84.7
Msa1235720 MtrunA17_Chr2g0311651 36.154 130 74 5 10 134 25 150 5.05e-20 84.0
Msa1235720 MtrunA17_Chr6g0453131 39.394 132 70 6 7 135 16 140 6.53e-20 84.7
Msa1235720 MtrunA17_Chr5g0417001 34.884 129 76 4 10 135 17 140 4.71e-19 82.0
Msa1235720 MtrunA17_Chr8g0384351 35.714 140 69 5 2 140 5 124 1.36e-17 75.5
Msa1235720 MtrunA17_Chr5g0442771 36.232 138 78 7 2 135 14 145 4.07e-17 76.3
Msa1235720 MtrunA17_Chr4g0050701 31.111 135 71 5 7 135 11 129 1.02e-16 73.2
Msa1235720 MtrunA17_Chr7g0251651 27.941 136 88 4 2 134 13 141 1.46e-13 65.1
Msa1235720 MtrunA17_Chr3g0130321 34.058 138 77 5 1 135 1 127 8.36e-13 63.2
Msa1235720 MtrunA17_Chr3g0113001 48.101 79 34 5 60 135 15 89 1.28e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1235720 AT4G35580.3 50.000 138 61 4 6 140 8 140 3.39e-38 136
Msa1235720 AT4G35580.2 50.000 138 61 4 6 140 8 140 3.79e-38 136
Msa1235720 AT4G35580.1 50.000 138 61 4 6 140 8 140 4.22e-38 135
Msa1235720 AT1G33060.2 48.148 135 62 4 6 137 23 152 8.18e-35 127
Msa1235720 AT1G33060.1 48.148 135 62 4 6 137 23 152 8.23e-35 127
Msa1235720 AT5G24590.2 46.043 139 68 4 5 140 11 145 1.35e-34 125
Msa1235720 AT3G49530.1 44.928 138 69 4 6 140 12 145 4.49e-34 124
Msa1235720 AT3G49530.2 44.928 138 69 4 6 140 12 145 1.32e-33 122
Msa1235720 AT4G01520.1 43.137 153 75 6 8 150 5 155 6.18e-33 118
Msa1235720 AT4G01540.2 44.737 152 72 6 9 150 6 155 2.44e-32 119
Msa1235720 AT4G01540.4 44.737 152 72 6 9 150 6 155 3.04e-32 118
Msa1235720 AT4G01540.1 44.737 152 72 6 9 150 6 155 3.53e-32 119
Msa1235720 AT4G01540.3 44.737 152 72 6 9 150 6 155 3.68e-32 118
Msa1235720 AT3G10490.1 44.118 136 68 4 3 135 23 153 3.85e-32 114
Msa1235720 AT1G02230.1 41.722 151 78 6 8 150 4 152 4.02e-32 117
Msa1235720 AT4G17980.1 43.704 135 70 3 4 135 3 134 4.73e-32 115
Msa1235720 AT3G10480.2 45.926 135 65 4 3 134 23 152 7.60e-32 118
Msa1235720 AT3G10480.1 45.926 135 65 4 3 134 23 152 8.17e-32 117
Msa1235720 AT4G17980.2 43.704 135 70 3 4 135 3 134 9.62e-32 115
Msa1235720 AT4G01550.1 43.046 151 75 5 9 150 6 154 3.73e-31 116
Msa1235720 AT3G15170.1 44.928 138 68 5 1 135 14 146 9.40e-31 112
Msa1235720 AT3G10490.2 44.118 136 68 4 3 135 23 153 1.70e-30 114
Msa1235720 AT5G04410.1 46.617 133 63 4 6 135 8 135 1.74e-30 115
Msa1235720 AT5G53950.1 45.185 135 66 5 4 135 14 143 2.33e-30 113
Msa1235720 AT2G27300.1 45.255 137 65 6 1 134 9 138 3.89e-30 111
Msa1235720 AT3G17730.1 43.662 142 69 5 6 144 5 138 4.22e-30 109
Msa1235720 AT5G17260.1 45.113 133 65 4 5 134 4 131 1.01e-29 112
Msa1235720 AT5G22290.1 45.985 137 64 6 1 134 16 145 1.10e-29 110
Msa1235720 AT5G46590.2 42.222 135 71 4 4 135 3 133 1.28e-29 109
Msa1235720 AT1G65910.1 45.865 133 64 4 5 134 4 131 1.40e-29 113
Msa1235720 AT5G46590.1 42.222 135 71 4 4 135 3 133 1.43e-29 109
Msa1235720 AT3G10500.1 45.865 133 64 4 6 135 8 135 5.63e-29 110
Msa1235720 AT2G24430.2 43.939 132 66 5 6 134 15 141 2.52e-28 106
Msa1235720 AT2G24430.1 43.939 132 66 5 6 134 15 141 2.52e-28 106
Msa1235720 AT5G61430.1 42.336 137 69 6 2 135 11 140 3.71e-28 106
Msa1235720 AT3G44290.2 46.094 128 60 5 10 134 17 138 6.55e-28 103
Msa1235720 AT1G01720.1 43.151 146 72 5 1 143 1 138 6.64e-28 105
Msa1235720 AT3G03200.1 41.611 149 76 5 5 150 4 144 8.19e-28 107
Msa1235720 AT3G18400.1 42.424 132 66 5 7 135 5 129 1.20e-27 104
Msa1235720 AT1G54330.1 45.185 135 66 4 6 137 5 134 1.44e-27 104
Msa1235720 AT5G04395.1 37.736 159 81 5 6 149 27 182 1.51e-27 101
Msa1235720 AT3G44290.1 46.094 128 60 5 10 134 17 138 8.04e-27 102
Msa1235720 AT5G07680.2 42.424 132 66 6 7 135 3 127 2.46e-26 101
Msa1235720 AT5G07680.1 42.424 132 66 6 7 135 17 141 3.04e-26 101
Msa1235720 AT3G10480.3 36.905 168 65 5 3 134 23 185 5.02e-26 102
Msa1235720 AT3G04060.1 40.458 131 69 5 7 134 20 144 5.41e-26 100
Msa1235720 AT1G77450.1 42.568 148 72 6 1 143 2 141 7.50e-26 99.0
Msa1235720 AT3G29035.1 45.113 133 61 7 7 135 24 148 8.22e-26 100
Msa1235720 AT5G08790.1 39.189 148 79 5 1 145 1 140 8.25e-26 99.4
Msa1235720 AT5G18270.2 40.876 137 72 5 2 135 16 146 8.46e-26 100
Msa1235720 AT1G71930.2 39.552 134 72 5 5 134 7 135 1.10e-25 99.8
Msa1235720 AT1G71930.1 39.552 134 72 5 5 134 7 135 1.10e-25 99.8
Msa1235720 AT5G62380.2 39.855 138 74 5 1 134 1 133 1.71e-25 99.8
Msa1235720 AT5G62380.1 39.855 138 74 5 1 134 1 133 1.71e-25 99.8
Msa1235720 AT5G39610.1 40.816 147 74 7 2 145 15 151 2.01e-25 98.2
Msa1235720 AT1G69490.1 40.000 135 71 5 3 134 5 132 3.04e-25 97.4
Msa1235720 AT1G02250.1 41.353 133 74 4 8 138 4 134 3.20e-25 99.0
Msa1235720 AT5G18270.1 39.416 137 74 5 2 135 16 146 3.51e-25 98.6
Msa1235720 AT1G32510.1 41.176 136 69 4 7 134 6 138 3.84e-25 97.4
Msa1235720 AT1G34180.3 44.531 128 63 4 10 134 19 141 5.36e-25 99.4
Msa1235720 AT1G34190.1 41.406 128 67 4 10 134 19 141 6.62e-25 99.8
Msa1235720 AT1G76420.1 40.602 133 69 6 6 134 21 147 7.09e-25 97.8
Msa1235720 AT1G34180.1 43.750 128 64 4 10 134 19 141 7.46e-25 99.4
Msa1235720 AT5G13180.1 38.636 132 70 4 7 135 14 137 1.03e-24 95.9
Msa1235720 AT4G36160.1 38.129 139 77 5 1 135 4 137 1.99e-24 97.1
Msa1235720 AT4G36160.3 38.129 139 77 5 1 135 4 137 1.99e-24 97.1
Msa1235720 AT4G36160.2 38.129 139 77 5 1 135 8 141 2.16e-24 97.1
Msa1235720 AT3G04070.2 42.336 137 68 5 5 135 8 139 2.26e-24 96.7
Msa1235720 AT3G15500.1 41.429 140 72 5 6 142 13 145 2.89e-24 95.9
Msa1235720 AT3G04070.1 43.066 137 67 5 5 135 8 139 3.13e-24 96.3
Msa1235720 AT1G62700.1 38.621 145 80 5 1 141 1 140 3.55e-24 96.7
Msa1235720 AT1G62700.2 38.621 145 80 5 1 141 1 140 3.55e-24 96.7
Msa1235720 AT5G63790.1 39.130 138 73 5 1 135 44 173 3.72e-24 95.5
Msa1235720 AT5G63790.2 39.130 138 73 5 1 135 54 183 3.98e-24 95.5
Msa1235720 AT5G66300.1 37.226 137 77 5 4 136 9 140 4.44e-24 95.1
Msa1235720 AT2G17040.1 40.441 136 70 6 10 141 9 137 5.09e-24 94.4
Msa1235720 AT1G12260.2 37.241 145 82 5 1 141 1 140 6.22e-24 95.5
Msa1235720 AT5G39820.1 38.462 143 71 5 10 140 23 160 7.81e-24 95.1
Msa1235720 AT1G52890.1 40.559 143 75 5 3 142 10 145 8.10e-24 94.7
Msa1235720 AT2G33480.1 39.394 132 67 5 7 135 15 136 8.78e-24 93.6
Msa1235720 AT2G33480.2 39.394 132 67 5 7 135 15 136 8.94e-24 93.6
Msa1235720 AT5G09330.5 42.969 128 65 4 10 134 9 131 1.03e-23 95.9
Msa1235720 AT5G09330.2 42.969 128 65 4 10 134 9 131 1.03e-23 95.9
Msa1235720 AT5G09330.4 42.969 128 65 4 10 134 9 131 1.03e-23 95.9
Msa1235720 AT5G09330.3 42.969 128 65 4 10 134 9 131 1.03e-23 95.9
Msa1235720 AT5G09330.1 42.969 128 65 4 10 134 9 131 1.03e-23 95.9
Msa1235720 AT2G18060.3 38.235 136 75 5 4 135 6 136 1.08e-23 95.1
Msa1235720 AT2G18060.1 38.235 136 75 5 4 135 6 136 1.08e-23 95.1
Msa1235720 AT1G12260.1 38.406 138 76 5 1 134 1 133 1.44e-23 95.1
Msa1235720 AT4G27410.2 42.105 133 67 5 6 135 13 138 1.79e-23 93.6
Msa1235720 AT3G15510.1 41.732 127 66 4 11 134 21 142 2.09e-23 94.4
Msa1235720 AT3G61910.1 36.296 135 77 5 5 135 9 138 4.58e-23 92.8
Msa1235720 AT3G04420.1 37.500 152 82 6 8 150 4 151 7.83e-23 92.4
Msa1235720 AT1G56010.2 36.842 133 75 4 5 134 17 143 7.84e-23 92.0
Msa1235720 AT3G04420.2 37.500 152 82 6 8 150 4 151 7.95e-23 91.7
Msa1235720 AT3G04430.1 40.000 140 66 5 13 139 6 140 9.03e-23 89.4
Msa1235720 AT1G61110.1 42.647 136 65 6 7 137 16 143 1.27e-22 91.7
Msa1235720 AT4G28530.1 33.758 157 82 4 2 140 5 157 1.29e-22 92.0
Msa1235720 AT2G02450.1 41.221 131 67 6 7 134 52 175 1.39e-22 92.0
Msa1235720 AT2G02450.2 41.221 131 67 6 7 134 52 175 1.96e-22 92.0
Msa1235720 AT1G01010.1 37.226 137 72 4 9 135 5 137 3.08e-22 91.7
Msa1235720 AT1G33280.1 35.616 146 85 5 3 144 4 144 7.98e-22 89.4
Msa1235720 AT4G27410.3 37.838 148 69 5 6 135 13 155 9.06e-22 89.4
Msa1235720 AT1G34180.2 40.000 140 64 5 10 134 19 153 1.01e-21 90.5
Msa1235720 AT2G46770.1 36.567 134 76 5 5 134 14 142 1.50e-21 89.4
Msa1235720 AT1G34180.4 40.000 140 64 5 10 134 19 153 1.67e-21 89.7
Msa1235720 AT3G12977.1 36.567 134 73 6 5 134 16 141 2.63e-21 87.4
Msa1235720 AT1G79580.3 36.620 142 82 5 6 143 16 153 2.75e-21 88.6
Msa1235720 AT1G79580.5 36.620 142 82 5 6 143 16 153 2.75e-21 88.6
Msa1235720 AT1G79580.4 36.620 142 82 5 6 143 16 153 2.75e-21 88.6
Msa1235720 AT1G79580.2 36.620 142 82 5 6 143 16 153 2.75e-21 88.6
Msa1235720 AT1G79580.1 36.620 142 82 5 6 143 16 153 2.75e-21 88.6
Msa1235720 AT4G10350.1 37.500 136 76 5 3 134 5 135 4.01e-21 87.8
Msa1235720 AT3G12910.1 36.232 138 77 6 2 134 17 148 4.33e-21 87.0
Msa1235720 AT1G32870.1 40.625 128 66 4 10 134 13 133 6.15e-21 88.2
Msa1235720 AT1G32870.2 38.806 134 72 4 4 134 7 133 6.54e-21 88.2
Msa1235720 AT1G32870.3 38.806 134 72 4 4 134 43 169 7.59e-21 88.2
Msa1235720 AT1G26870.1 37.500 128 72 4 10 134 19 141 1.21e-20 87.0
Msa1235720 AT5G64060.1 38.760 129 71 5 10 135 9 132 3.98e-20 85.1
Msa1235720 AT5G22380.1 37.037 135 74 6 9 136 7 137 6.70e-20 82.8
Msa1235720 AT3G44350.1 31.852 135 84 4 7 136 5 136 8.26e-20 80.9
Msa1235720 AT5G64530.2 37.037 135 69 5 6 140 2 120 1.05e-19 80.5
Msa1235720 AT2G43000.1 35.036 137 78 7 2 135 14 142 1.15e-19 82.8
Msa1235720 AT5G64530.1 37.956 137 65 6 6 140 2 120 1.90e-19 80.5
Msa1235720 AT1G32770.1 35.556 135 78 5 4 134 13 142 2.75e-19 82.8
Msa1235720 AT3G44350.2 31.852 135 84 4 7 136 5 136 4.55e-19 80.9
Msa1235720 AT1G02220.1 36.306 157 84 7 1 150 1 148 7.43e-19 82.0
Msa1235720 AT5G14000.2 38.346 133 68 5 7 134 15 138 3.91e-17 73.9
Msa1235720 AT5G14000.1 38.346 133 68 5 7 134 15 138 1.11e-16 73.9
Msa1235720 AT1G52880.1 41.727 139 65 7 11 142 21 150 1.23e-16 75.5
Msa1235720 AT4G28530.2 32.759 116 59 3 2 102 5 116 3.81e-13 65.5
Msa1235720 AT3G12977.2 39.726 73 37 3 65 134 20 88 4.25e-11 59.3
Msa1235720 AT3G56530.1 32.331 133 81 5 6 134 51 178 6.41e-11 59.3

Find 25 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCAAGCAACTTGTGATTT+AGG 0.277246 8_2:-13898440 None:intergenic
AAAGGCACCAATAATGTTAT+AGG 0.319995 8_2:+13898838 Msa1235720:CDS
CTATGCACCATGGGATTTAC+CGG 0.320849 8_2:+13898509 Msa1235720:CDS
GCAACACATACCTTGTTATC+AGG 0.351004 8_2:-13898940 None:intergenic
CTCACAAGTTCTTGATCTGT+GGG 0.450954 8_2:-13898411 None:intergenic
CTCAACGTGAAAAGACCAAC+TGG 0.473390 8_2:+13898890 Msa1235720:CDS
GATCGTGAAATCAAGACAAA+AGG 0.482376 8_2:+13898820 Msa1235720:CDS
TAAAATCAAATGATTCAGAA+TGG 0.484357 8_2:+13898701 Msa1235720:CDS
GTGATCTTCCAATAACCATC+CGG 0.488271 8_2:-13898790 None:intergenic
TACCTAAATCACAAGTTGCT+TGG 0.497667 8_2:+13898438 Msa1235720:CDS
ACCTCAGCTTCAATGCCAAT+AGG 0.501292 8_2:+13898375 Msa1235720:CDS
GGGTGAAATCTATAGCCTAT+TGG 0.501855 8_2:-13898390 None:intergenic
ATTGAAGCTGAGGTCTCCAT+TGG 0.535295 8_2:-13898366 None:intergenic
ACTCACAAGTTCTTGATCTG+TGG 0.552186 8_2:-13898412 None:intergenic
GCCTATTGGCATTGAAGCTG+AGG 0.555616 8_2:-13898376 None:intergenic
GATGGTTATTGGAAGATCAC+TGG 0.561459 8_2:+13898793 Msa1235720:CDS
ACATACCTTGTTATCAGGAA+AGG 0.564840 8_2:-13898935 None:intergenic
TGATCTTCCAATAACCATCC+GGG 0.567415 8_2:-13898789 None:intergenic
TTCAACCTTTCCTGATAACA+AGG 0.579529 8_2:+13898930 Msa1235720:CDS
ATACTCATGCATAATCCAGT+TGG 0.586289 8_2:-13898905 None:intergenic
ATATTACCCGGTAAATCCCA+TGG 0.627670 8_2:-13898516 None:intergenic
AGTTCAACAAAAGCAAAACA+AGG 0.647211 8_2:+13898752 Msa1235720:CDS
TATGCACCATGGGATTTACC+GGG 0.653252 8_2:+13898510 Msa1235720:CDS
TAGTTTAAAAGATGAACATG+AGG 0.670226 8_2:-13898489 None:intergenic
TCACAAGTTCTTGATCTGTG+GGG 0.733590 8_2:-13898410 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTATTTTTCGCATTA+AGG - chr8_2:13898551-13898570 None:intergenic 15.0%
!!! TTTTATGGGATTATATTAAA+TGG + chr8_2:13898602-13898621 Msa1235720:intron 15.0%
!!! TTTTCTGTTTCTACTTTTAT+GGG + chr8_2:13898588-13898607 Msa1235720:intron 20.0%
!! TAAAATCAAATGATTCAGAA+TGG + chr8_2:13898701-13898720 Msa1235720:CDS 20.0%
!!! TTTTATGCCTATAACATTAT+TGG - chr8_2:13898848-13898867 None:intergenic 20.0%
! TAGTTTAAAAGATGAACATG+AGG - chr8_2:13898492-13898511 None:intergenic 25.0%
!!! CTTTTCTGTTTCTACTTTTA+TGG + chr8_2:13898587-13898606 Msa1235720:intron 25.0%
! TATGGGATTATATTAAATGG+TGG + chr8_2:13898605-13898624 Msa1235720:intron 25.0%
! AAGGTTTAAAAGAAAAAACC+CGG + chr8_2:13898771-13898790 Msa1235720:CDS 25.0%
!!! GTATGTGTTGCTTTATTTTA+TGG + chr8_2:13898952-13898971 Msa1235720:CDS 25.0%
! ATCTTTTAAACTATGCACCA+TGG + chr8_2:13898499-13898518 Msa1235720:CDS 30.0%
! TCTTTTAAACTATGCACCAT+GGG + chr8_2:13898500-13898519 Msa1235720:CDS 30.0%
AGGAAGTAGAATATATTACC+CGG - chr8_2:13898531-13898550 None:intergenic 30.0%
AGTTCAACAAAAGCAAAACA+AGG + chr8_2:13898752-13898771 Msa1235720:CDS 30.0%
TTTAAAAGAAAAAACCCGGA+TGG + chr8_2:13898775-13898794 Msa1235720:CDS 30.0%
!! AAAGGCACCAATAATGTTAT+AGG + chr8_2:13898838-13898857 Msa1235720:CDS 30.0%
TACCTAAATCACAAGTTGCT+TGG + chr8_2:13898438-13898457 Msa1235720:CDS 35.0%
GATCGTGAAATCAAGACAAA+AGG + chr8_2:13898820-13898839 Msa1235720:CDS 35.0%
ATACTCATGCATAATCCAGT+TGG - chr8_2:13898908-13898927 None:intergenic 35.0%
TTCAACCTTTCCTGATAACA+AGG + chr8_2:13898930-13898949 Msa1235720:CDS 35.0%
! ACATACCTTGTTATCAGGAA+AGG - chr8_2:13898938-13898957 None:intergenic 35.0%
GGGTGAAATCTATAGCCTAT+TGG - chr8_2:13898393-13898412 None:intergenic 40.0%
TCACAAGTTCTTGATCTGTG+GGG - chr8_2:13898413-13898432 None:intergenic 40.0%
CTCACAAGTTCTTGATCTGT+GGG - chr8_2:13898414-13898433 None:intergenic 40.0%
ACTCACAAGTTCTTGATCTG+TGG - chr8_2:13898415-13898434 None:intergenic 40.0%
CACCAAGCAACTTGTGATTT+AGG - chr8_2:13898443-13898462 None:intergenic 40.0%
ATATTACCCGGTAAATCCCA+TGG - chr8_2:13898519-13898538 None:intergenic 40.0%
GAAAAAACCCGGATGGTTAT+TGG + chr8_2:13898782-13898801 Msa1235720:CDS 40.0%
TGATCTTCCAATAACCATCC+GGG - chr8_2:13898792-13898811 None:intergenic 40.0%
GTGATCTTCCAATAACCATC+CGG - chr8_2:13898793-13898812 None:intergenic 40.0%
GATGGTTATTGGAAGATCAC+TGG + chr8_2:13898793-13898812 Msa1235720:CDS 40.0%
! GCAACACATACCTTGTTATC+AGG - chr8_2:13898943-13898962 None:intergenic 40.0%
ACCTCAGCTTCAATGCCAAT+AGG + chr8_2:13898375-13898394 Msa1235720:CDS 45.0%
CTATGCACCATGGGATTTAC+CGG + chr8_2:13898509-13898528 Msa1235720:CDS 45.0%
TATGCACCATGGGATTTACC+GGG + chr8_2:13898510-13898529 Msa1235720:CDS 45.0%
!!! CAGTTGGTCTTTTCACGTTG+AGG - chr8_2:13898892-13898911 None:intergenic 45.0%
CTCAACGTGAAAAGACCAAC+TGG + chr8_2:13898890-13898909 Msa1235720:CDS 45.0%
! GCCTATTGGCATTGAAGCTG+AGG - chr8_2:13898379-13898398 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 13898369 13898978 13898369 ID=Msa1235720;Name=Msa1235720
chr8_2 mRNA 13898369 13898978 13898369 ID=Msa1235720-mRNA-1;Parent=Msa1235720;Name=Msa1235720-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|1|1|2|0|150
chr8_2 exon 13898369 13898531 13898369 ID=Msa1235720-mRNA-1:exon:5347;Parent=Msa1235720-mRNA-1
chr8_2 exon 13898689 13898978 13898689 ID=Msa1235720-mRNA-1:exon:5348;Parent=Msa1235720-mRNA-1
chr8_2 CDS 13898369 13898531 13898369 ID=Msa1235720-mRNA-1:cds;Parent=Msa1235720-mRNA-1
chr8_2 CDS 13898689 13898978 13898689 ID=Msa1235720-mRNA-1:cds;Parent=Msa1235720-mRNA-1
Gene Sequence

>Msa1235720

ATGGAGACCTCAGCTTCAATGCCAATAGGCTATAGATTTCACCCCACAGATCAAGAACTTGTGAGTCACTACCTAAATCACAAGTTGCTTGGTGATGATTCTATTGTCAGCAACATAATACCTCATGTTCATCTTTTAAACTATGCACCATGGGATTTACCGGCATTGTCAAAGTTAAAATCAAATGATTCAGAATGGTTTTTCTTCACTTATCTTGAACTGATGAAGTTCAACAAAAGCAAAACAAGGTTTAAAAGAAAAAACCCGGATGGTTATTGGAAGATCACTGGCAAAGATCGTGAAATCAAGACAAAAGGCACCAATAATGTTATAGGCATAAAAAAGACTCTTATTTTCTGTAGTCCTCAACGTGAAAAGACCAACTGGATTATGCATGAGTATCATTCAACCTTTCCTGATAACAAGGTATGTGTTGCTTTATTTTATGGATAA
Protein sequence

>Msa1235720

METSASMPIGYRFHPTDQELVSHYLNHKLLGDDSIVSNIIPHVHLLNYAPWDLPALSKLKSNDSEWFFFTYLELMKFNKSKTRFKRKNPDGYWKITGKDREIKTKGTNNVIGIKKTLIFCSPQREKTNWIMHEYHSTFPDNKVCVALFYG*