AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1241650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1241650 MtrunA17_Chr8g0354281 63.529 85 21 1 1 85 505 579 7.48e-28 105
Msa1241650 MtrunA17_Chr8g0354351 61.176 85 23 1 1 85 503 577 5.84e-27 103
Msa1241650 MtrunA17_Chr8g0354391 60.000 85 24 1 1 85 104 178 8.58e-27 99.4
Msa1241650 MtrunA17_Chr8g0354441 58.824 85 25 1 1 85 520 594 1.73e-26 102
Msa1241650 MtrunA17_Chr8g0354331 58.824 85 25 1 1 85 270 344 7.39e-26 99.4
Msa1241650 MtrunA17_Chr8g0354421 58.824 85 25 1 1 85 533 607 9.46e-26 100
Msa1241650 MtrunA17_Chr8g0354431 58.824 85 25 1 1 85 512 586 1.25e-25 99.8
Msa1241650 MtrunA17_Chr3g0081001 51.765 85 31 1 1 85 513 587 8.49e-21 85.9
Msa1241650 MtrunA17_Chr5g0394011 41.270 126 51 5 1 108 521 641 1.30e-18 79.7
Msa1241650 MtrunA17_Chr3g0082941 47.059 85 35 1 1 85 343 417 2.83e-18 78.6
Msa1241650 MtrunA17_Chr8g0354271 70.833 48 14 0 1 48 505 552 3.09e-18 78.6
Msa1241650 MtrunA17_Chr3g0081031 50.602 83 31 1 1 83 522 594 5.18e-18 77.8
Msa1241650 MtrunA17_Chr3g0081011 51.899 79 28 1 1 79 509 577 5.76e-18 77.8
Msa1241650 MtrunA17_Chr3g0081071 73.469 49 13 0 1 49 99 147 7.97e-18 75.9
Msa1241650 MtrunA17_Chr7g0236471 47.423 97 31 3 1 90 510 593 6.17e-17 74.7
Msa1241650 MtrunA17_Chr3g0081101 46.914 81 33 1 5 85 12 82 3.38e-16 69.7
Msa1241650 MtrunA17_Chr7g0236691 48.810 84 31 2 1 72 670 753 1.01e-15 71.2
Msa1241650 MtrunA17_Chr7g0236551 71.739 46 13 0 1 46 658 703 1.58e-15 70.9
Msa1241650 MtrunA17_Chr7g0236531 69.388 49 15 0 1 49 208 256 3.30e-15 69.7
Msa1241650 MtrunA17_Chr8g0357651 43.529 85 38 1 1 85 640 714 4.51e-15 69.7
Msa1241650 MtrunA17_Chr2g0280691 41.818 110 47 4 1 106 696 792 4.85e-15 69.3
Msa1241650 MtrunA17_Chr8g0361161 38.333 120 58 3 1 107 214 330 6.53e-15 68.9
Msa1241650 MtrunA17_Chr5g0394021 37.288 118 63 2 1 108 491 607 6.53e-15 68.9
Msa1241650 MtrunA17_Chr7g0236561 46.392 97 32 3 1 90 675 758 6.93e-15 68.9
Msa1241650 MtrunA17_Chr8g0361281 62.500 48 18 0 1 48 683 730 7.00e-15 68.9
Msa1241650 MtrunA17_Chr8g0361141 38.333 120 58 3 1 107 686 802 7.22e-15 68.9
Msa1241650 MtrunA17_Chr4g0048631 35.593 118 65 2 1 107 702 819 7.66e-15 68.9
Msa1241650 MtrunA17_Chr2g0323231 38.655 119 60 3 1 107 348 465 8.48e-15 68.6
Msa1241650 MtrunA17_Chr4g0023221 67.391 46 15 0 1 46 900 945 8.98e-15 68.6
Msa1241650 MtrunA17_Chr8g0361441 62.500 48 18 0 1 48 676 723 9.84e-15 68.6
Msa1241650 MtrunA17_Chr4g0048801 44.706 85 37 2 1 85 688 762 2.12e-14 67.8
Msa1241650 MtrunA17_Chr2g0280711 67.391 46 15 0 1 46 700 745 2.23e-14 67.4
Msa1241650 MtrunA17_Chr8g0357691 59.184 49 20 0 1 49 648 696 3.06e-14 67.0
Msa1241650 MtrunA17_Chr7g0236641 65.217 46 16 0 1 46 695 740 3.07e-14 67.0
Msa1241650 MtrunA17_Chr7g0236201 47.368 95 33 4 1 90 640 722 4.65e-14 66.6
Msa1241650 MtrunA17_Chr7g0236121 47.368 95 33 4 1 90 674 756 4.65e-14 66.6
Msa1241650 MtrunA17_Chr8g0357831 43.529 85 38 2 1 85 362 436 5.18e-14 66.6
Msa1241650 MtrunA17_Chr8g0357631 42.353 85 39 2 1 85 638 712 5.22e-14 66.6
Msa1241650 MtrunA17_Chr2g0280681 65.306 49 17 0 1 49 671 719 5.40e-14 66.6
Msa1241650 MtrunA17_Chr7g0236631 67.442 43 14 0 1 43 24 66 7.65e-14 61.6
Msa1241650 MtrunA17_Chr4g0042401 72.500 40 11 0 1 40 673 712 7.88e-14 65.9
Msa1241650 MtrunA17_Chr2g0280761 44.578 83 35 3 1 82 1 73 8.01e-14 63.2
Msa1241650 MtrunA17_Chr3g0119041 45.349 86 37 2 1 86 510 585 8.52e-14 65.9
Msa1241650 MtrunA17_Chr4g0023111 65.217 46 16 0 1 46 884 929 9.42e-14 65.9
Msa1241650 MtrunA17_Chr4g0055011 65.217 46 16 0 1 46 241 286 9.82e-14 65.5
Msa1241650 MtrunA17_Chr8g0360201 67.500 40 13 0 1 40 576 615 9.85e-14 65.9
Msa1241650 MtrunA17_Chr1g0206331 42.553 94 40 2 1 90 505 588 1.08e-13 65.5
Msa1241650 MtrunA17_Chr7g0236231 68.889 45 12 1 1 45 688 730 1.15e-13 65.5
Msa1241650 MtrunA17_Chr7g0236341 32.203 118 68 2 1 107 688 804 1.17e-13 65.5
Msa1241650 MtrunA17_Chr3g0084691 62.500 48 18 0 1 48 692 739 1.20e-13 65.5
Msa1241650 MtrunA17_Chr5g0428111 62.500 48 18 0 1 48 673 720 1.38e-13 65.5
Msa1241650 MtrunA17_Chr4g0042441 63.043 46 17 0 1 46 670 715 1.51e-13 65.1
Msa1241650 MtrunA17_Chr3g0078551 60.417 48 19 0 1 48 694 741 1.72e-13 65.1
Msa1241650 MtrunA17_Chr8g0357781 57.143 49 21 0 1 49 1 49 1.92e-13 62.4
Msa1241650 MtrunA17_Chr8g0340071 65.217 46 16 0 1 46 673 718 1.98e-13 64.7
Msa1241650 MtrunA17_Chr8g0340111 64.706 51 15 1 1 51 675 722 1.98e-13 64.7
Msa1241650 MtrunA17_Chr8g0340081 65.217 46 16 0 1 46 677 722 2.53e-13 64.7
Msa1241650 MtrunA17_Chr3g0078521 62.500 48 18 0 1 48 689 736 2.74e-13 64.3
Msa1241650 MtrunA17_Chr3g0078541 62.500 48 18 0 1 48 700 747 2.74e-13 64.3
Msa1241650 MtrunA17_Chr5g0422921 47.761 67 33 1 1 65 668 734 3.38e-13 64.3
Msa1241650 MtrunA17_Chr3g0092301 61.224 49 19 0 1 49 100 148 3.49e-13 63.2
Msa1241650 MtrunA17_Chr8g0340091 64.706 51 15 1 1 51 702 749 3.67e-13 63.9
Msa1241650 MtrunA17_Chr5g0428101 58.333 48 20 0 1 48 671 718 4.23e-13 63.9
Msa1241650 MtrunA17_Chr3g0078531 62.500 48 18 0 1 48 697 744 4.86e-13 63.9
Msa1241650 MtrunA17_Chr4g0042431 72.973 37 10 0 1 37 1 37 5.63e-13 61.2
Msa1241650 MtrunA17_Chr2g0280751 75.000 40 10 0 1 40 696 735 6.63e-13 63.5
Msa1241650 MtrunA17_Chr8g0358141 42.353 85 39 3 1 85 248 322 7.31e-13 63.2
Msa1241650 MtrunA17_Chr4g0048581 77.778 36 8 0 1 36 701 736 8.05e-13 63.2
Msa1241650 MtrunA17_Chr5g0428571 61.538 52 20 0 1 52 468 519 8.69e-13 63.2
Msa1241650 MtrunA17_Chr3g0084441 62.500 48 18 0 1 48 676 723 9.30e-13 62.8
Msa1241650 MtrunA17_Chr8g0340051 67.391 46 15 0 1 46 199 244 9.91e-13 62.8
Msa1241650 MtrunA17_Chr7g0236651 75.676 37 9 0 1 37 655 691 1.00e-12 62.8
Msa1241650 MtrunA17_Chr1g0206361 43.750 80 35 1 1 80 513 582 1.20e-12 62.8
Msa1241650 MtrunA17_Chr2g0280791 72.973 37 10 0 1 37 691 727 1.30e-12 62.4
Msa1241650 MtrunA17_Chr2g0280801 72.973 37 10 0 1 37 697 733 1.30e-12 62.4
Msa1241650 MtrunA17_Chr1g0206321 63.415 41 15 0 1 41 1 41 1.34e-12 60.5
Msa1241650 MtrunA17_Chr3g0084411 60.417 48 19 0 1 48 669 716 1.56e-12 62.4
Msa1241650 MtrunA17_Chr2g0280831 72.973 37 10 0 1 37 265 301 1.56e-12 62.4
Msa1241650 MtrunA17_Chr3g0093161 72.093 43 10 1 1 43 667 707 1.80e-12 62.0
Msa1241650 MtrunA17_Chr2g0280781 62.222 45 15 1 1 45 680 722 2.08e-12 62.0
Msa1241650 MtrunA17_Chr4g0048731 41.250 80 37 1 1 80 216 285 2.20e-12 61.6
Msa1241650 MtrunA17_Chr8g0357881 58.333 48 20 0 1 48 646 693 2.55e-12 61.6
Msa1241650 MtrunA17_Chr3g0084451 60.417 48 19 0 1 48 676 723 2.66e-12 61.6
Msa1241650 MtrunA17_Chr3g0084491 60.417 48 19 0 1 48 676 723 2.71e-12 61.6
Msa1241650 MtrunA17_Chr8g0357661 40.000 85 41 2 1 85 33 107 2.81e-12 59.7
Msa1241650 MtrunA17_Chr8g0357901 50.909 55 27 0 1 55 18 72 3.09e-12 57.8
Msa1241650 MtrunA17_Chr1g0154371 63.265 49 18 0 1 49 526 574 3.27e-12 61.2
Msa1241650 MtrunA17_Chr3g0084421 58.333 48 20 0 1 48 632 679 3.79e-12 61.2
Msa1241650 MtrunA17_Chr8g0357761 40.000 85 41 2 1 85 650 724 4.15e-12 61.2
Msa1241650 MtrunA17_Chr8g0358121 58.333 48 20 0 1 48 632 679 4.18e-12 61.2
Msa1241650 MtrunA17_Chr8g0358101 58.333 48 20 0 1 48 634 681 4.22e-12 61.2
Msa1241650 MtrunA17_Chr1g0154311 64.444 45 16 0 1 45 521 565 4.38e-12 60.8
Msa1241650 MtrunA17_Chr3g0084431 58.333 48 20 0 1 48 664 711 4.85e-12 60.8
Msa1241650 MtrunA17_Chr3g0084631 57.143 49 21 0 1 49 33 81 4.92e-12 59.3
Msa1241650 MtrunA17_Chr8g0357981 58.333 48 20 0 1 48 475 522 6.54e-12 60.5
Msa1241650 MtrunA17_Chr8g0358071 58.333 48 20 0 1 48 646 693 6.68e-12 60.5
Msa1241650 MtrunA17_Chr6g0474991 41.250 80 38 3 1 80 33 103 7.69e-12 58.9
Msa1241650 MtrunA17_Chr8g0358081 56.250 48 21 0 1 48 631 678 7.79e-12 60.5
Msa1241650 MtrunA17_Chr3g0084681 58.333 48 20 0 1 48 634 681 1.01e-11 60.1
Msa1241650 MtrunA17_Chr8g0358161 56.250 48 21 0 1 48 216 263 1.10e-11 59.7
Msa1241650 MtrunA17_Chr8g0358061 57.143 49 21 0 1 49 226 274 1.24e-11 59.7
Msa1241650 MtrunA17_Chr3g0084661 52.459 61 28 1 1 61 203 262 1.29e-11 59.7
Msa1241650 MtrunA17_Chr2g0280701 60.870 46 18 0 1 46 670 715 1.44e-11 59.7
Msa1241650 MtrunA17_Chr6g0474871 43.750 80 36 4 1 80 735 805 1.58e-11 59.3
Msa1241650 MtrunA17_Chr8g0361261 36.134 119 62 3 1 107 707 823 1.62e-11 59.3
Msa1241650 MtrunA17_Chr5g0428521 53.448 58 25 1 1 56 170 227 1.69e-11 58.5
Msa1241650 MtrunA17_Chr2g0280731 68.293 41 13 0 1 41 720 760 1.76e-11 59.3
Msa1241650 MtrunA17_Chr4g0042351 78.788 33 7 0 1 33 623 655 2.02e-11 59.3
Msa1241650 MtrunA17_Chr8g0357991 58.333 48 20 0 1 48 655 702 2.17e-11 58.9
Msa1241650 MtrunA17_Chr4g0042361 67.568 37 12 0 1 37 709 745 2.20e-11 58.9
Msa1241650 MtrunA17_Chr1g0154331 62.222 45 17 0 1 45 209 253 2.44e-11 58.9
Msa1241650 MtrunA17_Chr4g0042381 78.788 33 7 0 1 33 623 655 2.57e-11 58.9
Msa1241650 MtrunA17_Chr4g0042391 78.788 33 7 0 1 33 623 655 2.57e-11 58.9
Msa1241650 MtrunA17_Chr2g0281241 44.444 72 40 0 1 72 224 295 2.63e-11 58.5
Msa1241650 MtrunA17_Chr7g0236571 34.211 114 62 3 6 107 510 622 2.74e-11 58.9
Msa1241650 MtrunA17_Chr8g0357711 54.167 48 22 0 1 48 647 694 3.29e-11 58.5
Msa1241650 MtrunA17_Chr8g0357961 56.250 48 21 0 1 48 654 701 3.29e-11 58.5
Msa1241650 MtrunA17_Chr4g0048711 57.143 49 21 0 1 49 99 147 3.34e-11 58.5
Msa1241650 MtrunA17_Chr8g0358031 58.333 48 20 0 1 48 649 696 3.39e-11 58.5
Msa1241650 MtrunA17_Chr6g0474901 41.975 81 36 2 1 80 736 806 3.48e-11 58.5
Msa1241650 MtrunA17_Chr8g0361341 34.167 120 63 3 1 107 8 124 4.75e-11 56.2
Msa1241650 MtrunA17_Chr5g0426941 56.863 51 20 1 1 51 509 557 5.69e-11 57.8
Msa1241650 MtrunA17_Chr8g0357911 62.162 37 14 0 1 37 75 111 5.91e-11 55.1
Msa1241650 MtrunA17_Chr6g0474811 42.500 80 37 4 1 80 743 813 5.94e-11 57.8
Msa1241650 MtrunA17_Chr8g0361301 35.294 119 63 3 1 107 676 792 6.44e-11 57.8
Msa1241650 MtrunA17_Chr6g0475091 60.976 41 16 0 1 41 751 791 6.88e-11 57.8
Msa1241650 MtrunA17_Chr5g0394001 63.043 46 17 0 1 46 500 545 7.32e-11 57.4
Msa1241650 MtrunA17_Chr6g0453561 58.696 46 19 0 1 46 37 82 7.54e-11 56.2
Msa1241650 MtrunA17_Chr8g0357871 51.020 49 24 0 1 49 1 49 7.72e-11 54.7
Msa1241650 MtrunA17_Chr5g0428581 60.870 46 18 0 1 46 214 259 8.45e-11 57.4
Msa1241650 MtrunA17_Chr6g0475131 38.824 85 43 2 1 85 179 254 9.72e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1241650 AT4G21366.1 71.739 46 13 0 1 46 1 46 1.58e-15 67.0
Msa1241650 AT4G21390.2 70.588 51 14 1 1 51 698 747 4.50e-15 70.1
Msa1241650 AT1G65790.1 69.565 46 14 0 1 46 688 733 5.78e-15 69.7
Msa1241650 AT1G65800.1 69.565 46 14 0 1 46 692 737 5.79e-15 69.7
Msa1241650 AT4G21380.1 69.565 46 14 0 1 46 695 740 9.05e-15 68.9
Msa1241650 AT4G23180.2 51.667 60 29 0 1 60 517 576 2.50e-14 67.8
Msa1241650 AT4G21390.1 58.209 67 25 2 1 67 698 761 3.81e-14 67.4
Msa1241650 AT4G23140.1 45.570 79 33 1 1 79 520 588 4.82e-14 67.0
Msa1241650 AT4G23140.2 45.570 79 33 1 1 79 526 594 4.83e-14 67.0
Msa1241650 AT4G27300.1 67.347 49 16 0 1 49 669 717 1.47e-13 65.5
Msa1241650 AT4G23160.1 46.667 75 30 1 1 75 528 592 1.61e-13 65.5
Msa1241650 AT4G27290.1 62.500 48 18 0 1 48 634 681 1.74e-13 65.5
Msa1241650 AT4G27290.2 62.500 48 18 0 1 48 680 727 1.75e-13 65.5
Msa1241650 AT4G23230.1 60.417 48 19 0 1 48 506 553 2.34e-13 65.1
Msa1241650 AT1G11340.3 63.462 52 13 2 1 50 752 799 4.07e-13 64.3
Msa1241650 AT4G23150.1 33.898 118 60 4 1 102 505 620 5.26e-13 63.9
Msa1241650 AT4G03230.3 33.613 119 67 2 1 107 694 812 6.09e-13 63.9
Msa1241650 AT4G03230.7 33.613 119 67 2 1 107 702 820 6.09e-13 63.9
Msa1241650 AT4G03230.2 33.613 119 67 2 1 107 759 877 6.12e-13 63.9
Msa1241650 AT4G03230.6 33.613 119 67 2 1 107 759 877 6.12e-13 63.9
Msa1241650 AT4G03230.5 33.613 119 67 2 1 107 787 905 6.13e-13 63.9
Msa1241650 AT4G03230.4 33.613 119 67 2 1 107 859 977 6.15e-13 63.9
Msa1241650 AT4G03230.1 33.613 119 67 2 1 107 859 977 6.15e-13 63.9
Msa1241650 AT4G03230.8 33.613 119 67 2 1 107 701 819 6.91e-13 63.5
Msa1241650 AT1G61610.1 47.500 80 29 3 1 79 694 761 8.55e-13 63.5
Msa1241650 AT1G61610.2 59.259 54 21 1 1 54 694 746 8.64e-13 63.5
Msa1241650 AT1G11350.1 75.676 37 9 0 1 37 681 717 9.88e-13 63.2
Msa1241650 AT4G21230.1 46.667 75 29 2 1 75 502 565 1.07e-12 63.2
Msa1241650 AT1G11340.1 65.909 44 11 1 1 44 684 723 1.47e-12 62.8
Msa1241650 AT1G11410.2 70.270 37 11 0 1 37 577 613 1.62e-12 62.8
Msa1241650 AT1G11410.3 70.270 37 11 0 1 37 668 704 1.64e-12 62.8
Msa1241650 AT1G11410.1 70.270 37 11 0 1 37 692 728 1.64e-12 62.8
Msa1241650 AT1G11410.4 70.270 37 11 0 1 37 699 735 1.64e-12 62.8
Msa1241650 AT4G00960.2 59.615 52 18 1 1 52 133 181 2.62e-12 61.6
Msa1241650 AT4G00970.1 59.615 52 18 1 1 52 515 563 2.72e-12 62.0
Msa1241650 AT4G00960.3 59.615 52 18 1 1 52 140 188 3.01e-12 61.6
Msa1241650 AT4G11470.1 46.914 81 32 2 1 80 508 578 3.75e-12 61.6
Msa1241650 AT4G11470.2 46.914 81 32 2 1 80 513 583 3.75e-12 61.6
Msa1241650 AT4G00960.1 59.615 52 18 1 1 52 227 275 3.99e-12 61.2
Msa1241650 AT4G00970.2 59.615 52 18 1 1 52 515 563 4.11e-12 61.6
Msa1241650 AT4G11530.1 45.679 81 33 3 1 80 521 591 4.34e-12 61.2
Msa1241650 AT4G23180.1 56.522 46 20 0 1 46 517 562 5.48e-12 61.2
Msa1241650 AT1G61380.2 60.870 46 18 0 1 46 510 555 5.63e-12 61.2
Msa1241650 AT1G61380.1 60.870 46 18 0 1 46 657 702 5.73e-12 61.2
Msa1241650 AT4G21410.1 64.583 48 15 1 1 46 403 450 1.19e-11 60.1
Msa1241650 AT4G21410.3 64.583 48 15 1 1 46 528 575 1.22e-11 60.1
Msa1241650 AT1G11303.1 34.234 111 69 1 1 107 678 788 1.27e-11 60.1
Msa1241650 AT1G11300.2 35.833 120 55 4 1 107 663 773 1.72e-11 59.7
Msa1241650 AT1G11300.1 35.833 120 55 4 1 107 678 788 1.72e-11 59.7
Msa1241650 AT1G61430.11 33.884 121 62 4 1 107 635 751 3.42e-11 58.9
Msa1241650 AT1G61430.12 33.884 121 62 4 1 107 635 751 3.42e-11 58.9
Msa1241650 AT1G61430.13 33.884 121 62 4 1 107 635 751 3.42e-11 58.9
Msa1241650 AT4G23190.2 46.429 56 30 0 1 56 519 574 3.46e-11 58.9
Msa1241650 AT4G05200.1 53.061 49 23 0 1 49 516 564 3.58e-11 58.9
Msa1241650 AT1G61430.1 33.884 121 62 4 1 107 661 777 3.85e-11 58.9
Msa1241650 AT1G61430.9 33.884 121 62 4 1 107 658 774 3.96e-11 58.5
Msa1241650 AT4G05200.2 53.061 49 23 0 1 49 534 582 3.99e-11 58.5
Msa1241650 AT4G23190.3 60.000 40 16 0 1 40 441 480 4.13e-11 58.5
Msa1241650 AT4G23190.4 60.000 40 16 0 1 40 441 480 4.13e-11 58.5
Msa1241650 AT4G23190.1 60.000 40 16 0 1 40 519 558 4.18e-11 58.5
Msa1241650 AT1G61430.10 54.000 50 23 0 1 50 635 684 4.40e-11 58.5
Msa1241650 AT1G61430.5 54.000 50 23 0 1 50 661 710 5.58e-11 58.2
Msa1241650 AT1G61430.8 54.000 50 23 0 1 50 658 707 5.85e-11 58.2
Msa1241650 AT1G61430.7 54.000 50 23 0 1 50 658 707 5.85e-11 58.2
Msa1241650 AT4G38830.1 58.696 46 19 0 1 46 514 559 6.16e-11 58.2
Msa1241650 AT1G11330.3 54.000 50 23 0 1 50 693 742 7.71e-11 57.8
Msa1241650 AT4G23200.1 30.833 120 69 4 1 107 505 623 7.77e-11 57.8
Msa1241650 AT1G61370.2 54.348 46 21 0 1 46 519 564 9.00e-11 57.8
Msa1241650 AT1G61370.1 54.348 46 21 0 1 46 670 715 9.15e-11 57.8

Find 19 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTTCCTTTCGGATCAT+AGG 0.325558 8_2:-25315309 Msa1241650:CDS
AATCCAGCCATAGCATATTC+TGG 0.348026 8_2:+25315406 None:intergenic
TAGAAATCGTTTATGGGAAA+AGG 0.365288 8_2:-25315339 Msa1241650:CDS
ATGTATGATTCTTTGTGAAT+TGG 0.375987 8_2:+25315074 None:intergenic
CAACAGTAAAATATTTATGC+TGG 0.441629 8_2:+25315046 None:intergenic
GAATGGTGGATTCTTCCTTT+CGG 0.452390 8_2:-25315317 Msa1241650:CDS
GTATGCAGACTTGGAAACTT+TGG 0.490105 8_2:-25315126 Msa1241650:intron
ATTCTTCCTTTCGGATCATA+GGG 0.501581 8_2:-25315308 Msa1241650:CDS
GCTCCAGAATATGCTATGGC+TGG 0.510881 8_2:-25315409 Msa1241650:CDS
CATGAGAACGACGGTGGACA+TGG 0.544570 8_2:+25314990 None:intergenic
ACTTGGAAACTTTGGTGCGA+AGG 0.557056 8_2:-25315118 Msa1241650:CDS
ATCGTTTATGGGAAAAGGAA+TGG 0.559052 8_2:-25315334 Msa1241650:CDS
TCCACCGTCGTTCTCATGCT+AGG 0.562635 8_2:-25314985 None:intergenic
CATGGCTCCAGAATATGCTA+TGG 0.587375 8_2:-25315413 Msa1241650:CDS
GTTTATGGGAAAAGGAATGG+TGG 0.591460 8_2:-25315331 Msa1241650:CDS
GACTTTCCCTATGATCCGAA+AGG 0.615688 8_2:+25315302 None:intergenic
AGAACGACGGTGGACATGGT+TGG 0.623486 8_2:+25314994 None:intergenic
TCCTAGCATGAGAACGACGG+TGG 0.657368 8_2:+25314984 None:intergenic
ACTTCCTAGCATGAGAACGA+CGG 0.712254 8_2:+25314981 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTAGAAATCGTTTAT+GGG - chr8_2:25315053-25315072 Msa1241650:CDS 15.0%
!!! TATATGTCATCATATATTTT+TGG + chr8_2:25315197-25315216 None:intergenic 15.0%
!!! CTTTTATTAGAAATCGTTTA+TGG - chr8_2:25315052-25315071 Msa1241650:CDS 20.0%
!!! ATCATATATTTTTGGATAGT+TGG + chr8_2:25315189-25315208 None:intergenic 20.0%
!!! AAATCTGATGTTTTTAGCTT+TGG - chr8_2:25315025-25315044 Msa1241650:CDS 25.0%
! ATGTATGATTCTTTGTGAAT+TGG + chr8_2:25315327-25315346 None:intergenic 25.0%
! CAACAGTAAAATATTTATGC+TGG + chr8_2:25315355-25315374 None:intergenic 25.0%
TAGAAATCGTTTATGGGAAA+AGG - chr8_2:25315059-25315078 Msa1241650:CDS 30.0%
!! TTCATTTTTGTATGCAGACT+TGG - chr8_2:25315263-25315282 Msa1241650:CDS 30.0%
ATCGTTTATGGGAAAAGGAA+TGG - chr8_2:25315064-25315083 Msa1241650:CDS 35.0%
ATTCTTCCTTTCGGATCATA+GGG - chr8_2:25315090-25315109 Msa1241650:CDS 35.0%
AATCCAGCCATAGCATATTC+TGG + chr8_2:25314995-25315014 None:intergenic 40.0%
GTTTATGGGAAAAGGAATGG+TGG - chr8_2:25315067-25315086 Msa1241650:CDS 40.0%
!! GAATGGTGGATTCTTCCTTT+CGG - chr8_2:25315081-25315100 Msa1241650:CDS 40.0%
GATTCTTCCTTTCGGATCAT+AGG - chr8_2:25315089-25315108 Msa1241650:CDS 40.0%
CTCATCTCTCATCAACGTAT+TGG - chr8_2:25315145-25315164 Msa1241650:intron 40.0%
GTATGCAGACTTGGAAACTT+TGG - chr8_2:25315272-25315291 Msa1241650:CDS 40.0%
TTGGTGCGAAGGAAAAAGTT+TGG - chr8_2:25315291-25315310 Msa1241650:CDS 40.0%
GACTTTCCCTATGATCCGAA+AGG + chr8_2:25315099-25315118 None:intergenic 45.0%
! ACTTGGAAACTTTGGTGCGA+AGG - chr8_2:25315280-25315299 Msa1241650:CDS 45.0%
GCTCCAGAATATGCTATGGC+TGG - chr8_2:25314989-25315008 Msa1241650:CDS 50.0%
AGAACGACGGTGGACATGGT+TGG + chr8_2:25315407-25315426 None:intergenic 55.0%
CATGAGAACGACGGTGGACA+TGG + chr8_2:25315411-25315430 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 25314986 25315434 25314986 ID=Msa1241650;Name=Msa1241650
chr8_2 mRNA 25314986 25315434 25314986 ID=Msa1241650-mRNA-1;Parent=Msa1241650;Name=Msa1241650-mRNA-1;_AED=0.57;_eAED=0.68;_QI=0|0|0|1|1|1|2|0|109
chr8_2 exon 25315260 25315434 25315260 ID=Msa1241650-mRNA-1:exon:8956;Parent=Msa1241650-mRNA-1
chr8_2 exon 25314986 25315140 25314986 ID=Msa1241650-mRNA-1:exon:8955;Parent=Msa1241650-mRNA-1
chr8_2 CDS 25315260 25315434 25315260 ID=Msa1241650-mRNA-1:cds;Parent=Msa1241650-mRNA-1
chr8_2 CDS 25314986 25315140 25314986 ID=Msa1241650-mRNA-1:cds;Parent=Msa1241650-mRNA-1
Gene Sequence

>Msa1241650

ATGGCTCCAGAATATGCTATGGCTGGATTATTTTCAGTGAAATCTGATGTTTTTAGCTTTGGAGTTCTTTTATTAGAAATCGTTTATGGGAAAAGGAATGGTGGATTCTTCCTTTCGGATCATAGGGAAAGTCTTCTATTTCATGTGAGTTTTCTTGATCTCATCTCTCATCAACACTTGGAAACTTTGGTGCGAAGGAAAAAGTTTGGATTAATTGATCCAATTCACAAAGAATCATACATTGAAACCAGCATAAATATTTTACTGTTGATGAAGTACAAGAAGATGCAGCAGATCGACCAACCATGTCCACCGTCGTTCTCATGCTAG
Protein sequence

>Msa1241650

MAPEYAMAGLFSVKSDVFSFGVLLLEIVYGKRNGGFFLSDHRESLLFHVSFLDLISHQHLETLVRRKKFGLIDPIHKESYIETSINILLLMKYKKMQQIDQPCPPSFSC*