Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1242550 | PNX61918.1 | 82.927 | 41 | 7 | 0 | 58 | 98 | 1 | 41 | 3.10e-16 | 76.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1242550 | sp|Q9LXT3|MBF1B_ARATH | 63.415 | 41 | 15 | 0 | 58 | 98 | 1 | 41 | 1.37e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1242550 | A0A2K3K6K1 | 82.927 | 41 | 7 | 0 | 58 | 98 | 1 | 41 | 1.48e-16 | 76.6 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1242550 | TR | MBF1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1242550 | MtrunA17_Chr2g0320211 | 86.486 | 37 | 5 | 0 | 62 | 98 | 3 | 39 | 2.14e-17 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1242550 | AT3G58680.1 | 63.415 | 41 | 15 | 0 | 58 | 98 | 1 | 41 | 1.39e-15 | 67.8 |
| Msa1242550 | AT2G42680.1 | 60.976 | 41 | 16 | 0 | 58 | 98 | 1 | 41 | 7.48e-14 | 63.2 |
Find 22 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGACCTGTTTCGCAATAT+TGG | 0.215735 | 8_2:+26848222 | Msa1242550:CDS |
| TATATACGTGTCAGGCAAAT+TGG | 0.289194 | 8_2:+26848070 | Msa1242550:CDS |
| CGGACCTGTTTCGCAATATT+GGG | 0.304490 | 8_2:+26848223 | Msa1242550:CDS |
| CGTTGTGTTGAATCCGTATA+GGG | 0.346368 | 8_2:-26848142 | None:intergenic |
| ATGGTTGAATGGTGTGAATT+TGG | 0.349568 | 8_2:-26848191 | None:intergenic |
| TCGTTGTGTTGAATCCGTAT+AGG | 0.364174 | 8_2:-26848143 | None:intergenic |
| TACTCATCTTTATCTTCTCC+TGG | 0.380813 | 8_2:+26848098 | Msa1242550:CDS |
| GGTTCCCAATATTGCGAAAC+AGG | 0.386489 | 8_2:-26848227 | None:intergenic |
| GTTTCTTCATGGTTTCGTTT+CGG | 0.388579 | 8_2:-26848330 | None:intergenic |
| GTCAACGCCGCTCGCCGTGC+CGG | 0.401385 | 8_2:+26848305 | Msa1242550:CDS |
| TCATGGTTTCGTTTCGGCAC+CGG | 0.436990 | 8_2:-26848324 | None:intergenic |
| TATCATTATATATACGTGTC+AGG | 0.449792 | 8_2:+26848062 | Msa1242550:CDS |
| CGGCAGTGGGAGCTTTCTTG+TGG | 0.481257 | 8_2:-26848259 | None:intergenic |
| TCACACCATTCAACCATGTC+AGG | 0.495534 | 8_2:+26848197 | Msa1242550:CDS |
| TTCGGCACCGGCACGGCGAG+CGG | 0.500665 | 8_2:-26848312 | None:intergenic |
| CGATTCCTGACATGGTTGAA+TGG | 0.523177 | 8_2:-26848202 | None:intergenic |
| GCTTTCTTGTGGAGAACGAT+GGG | 0.525575 | 8_2:-26848248 | None:intergenic |
| CATTCAACCATGTCAGGAAT+CGG | 0.526075 | 8_2:+26848203 | Msa1242550:CDS |
| AGCTTTCTTGTGGAGAACGA+TGG | 0.532846 | 8_2:-26848249 | None:intergenic |
| AACAGGTCCGATTCCTGACA+TGG | 0.556467 | 8_2:-26848210 | None:intergenic |
| GTTTCGTTTCGGCACCGGCA+CGG | 0.563829 | 8_2:-26848319 | None:intergenic |
| TTGTTAGAAATTATAAAACC+AGG | 0.584332 | 8_2:-26848116 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTTAGAAATTATAAAACC+AGG | - | chr8_2:26848119-26848138 | None:intergenic | 20.0% |
| ! | TATCATTATATATACGTGTC+AGG | + | chr8_2:26848062-26848081 | Msa1242550:CDS | 25.0% |
| ! | TTTCTAACAAAAACCCTATA+CGG | + | chr8_2:26848129-26848148 | Msa1242550:CDS | 25.0% |
| !!! | ATGGTGTGAATTTGGTTTTT+GGG | - | chr8_2:26848186-26848205 | None:intergenic | 30.0% |
| !!! | AATGGTGTGAATTTGGTTTT+TGG | - | chr8_2:26848187-26848206 | None:intergenic | 30.0% |
| TATATACGTGTCAGGCAAAT+TGG | + | chr8_2:26848070-26848089 | Msa1242550:CDS | 35.0% | |
| TACTCATCTTTATCTTCTCC+TGG | + | chr8_2:26848098-26848117 | Msa1242550:CDS | 35.0% | |
| !!! | TGGTGTGAATTTGGTTTTTG+GGG | - | chr8_2:26848185-26848204 | None:intergenic | 35.0% |
| ATGGTTGAATGGTGTGAATT+TGG | - | chr8_2:26848194-26848213 | None:intergenic | 35.0% | |
| ! | GACAACTTTGTCATCTTTTC+TGG | - | chr8_2:26848288-26848307 | None:intergenic | 35.0% |
| CGTTGTGTTGAATCCGTATA+GGG | - | chr8_2:26848145-26848164 | None:intergenic | 40.0% | |
| TCGTTGTGTTGAATCCGTAT+AGG | - | chr8_2:26848146-26848165 | None:intergenic | 40.0% | |
| CATTCAACCATGTCAGGAAT+CGG | + | chr8_2:26848203-26848222 | Msa1242550:CDS | 40.0% | |
| !! | TTTGTCATCTTTTCTGGCAG+CGG | - | chr8_2:26848282-26848301 | None:intergenic | 40.0% |
| TCACACCATTCAACCATGTC+AGG | + | chr8_2:26848197-26848216 | Msa1242550:CDS | 45.0% | |
| CGATTCCTGACATGGTTGAA+TGG | - | chr8_2:26848205-26848224 | None:intergenic | 45.0% | |
| TCGGACCTGTTTCGCAATAT+TGG | + | chr8_2:26848222-26848241 | Msa1242550:CDS | 45.0% | |
| CGGACCTGTTTCGCAATATT+GGG | + | chr8_2:26848223-26848242 | Msa1242550:CDS | 45.0% | |
| GGTTCCCAATATTGCGAAAC+AGG | - | chr8_2:26848230-26848249 | None:intergenic | 45.0% | |
| GCTTTCTTGTGGAGAACGAT+GGG | - | chr8_2:26848251-26848270 | None:intergenic | 45.0% | |
| AGCTTTCTTGTGGAGAACGA+TGG | - | chr8_2:26848252-26848271 | None:intergenic | 45.0% | |
| AACAGGTCCGATTCCTGACA+TGG | - | chr8_2:26848213-26848232 | None:intergenic | 50.0% | |
| TCATGGTTTCGTTTCGGCAC+CGG | - | chr8_2:26848327-26848346 | None:intergenic | 50.0% | |
| !! | TCTTTTCTGGCAGCGGCAGT+GGG | - | chr8_2:26848275-26848294 | None:intergenic | 55.0% |
| !! | ATCTTTTCTGGCAGCGGCAG+TGG | - | chr8_2:26848276-26848295 | None:intergenic | 55.0% |
| CGGCAGTGGGAGCTTTCTTG+TGG | - | chr8_2:26848262-26848281 | None:intergenic | 60.0% | |
| !! | GTTTCGTTTCGGCACCGGCA+CGG | - | chr8_2:26848322-26848341 | None:intergenic | 60.0% |
| GTCAACGCCGCTCGCCGTGC+CGG | + | chr8_2:26848305-26848324 | Msa1242550:CDS | 75.0% | |
| !! | TTCGGCACCGGCACGGCGAG+CGG | - | chr8_2:26848315-26848334 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 26848041 | 26848346 | 26848041 | ID=Msa1242550;Name=Msa1242550 |
| chr8_2 | mRNA | 26848041 | 26848346 | 26848041 | ID=Msa1242550-mRNA-1;Parent=Msa1242550;Name=Msa1242550-mRNA-1;_AED=0.76;_eAED=0.76;_QI=0|-1|0|1|-1|1|1|0|101 |
| chr8_2 | exon | 26848041 | 26848346 | 26848041 | ID=Msa1242550-mRNA-1:exon:9438;Parent=Msa1242550-mRNA-1 |
| chr8_2 | CDS | 26848041 | 26848346 | 26848041 | ID=Msa1242550-mRNA-1:cds;Parent=Msa1242550-mRNA-1 |
| Gene Sequence |
| Protein sequence |
