AlfalfaGEDB | Msa1243410

M. sativa cultivar zhongmu-4 / Msa1243410

KEGG: K09338
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF04770.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1243410	XP_013445128.1	65.000	80	21	1	72	144	18	97	2.61e-28	111

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1243410	A0A072TNS1	65.000	80	21	1	72	144	18	97	1.25e-28	111

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TFs/TRs:

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Gene ID	Type	Classification
Msa1243410	TF	zf-HD

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1243410	MtrunA17_Chr8g0357721	65.000	80	21	1	72	144	18	97	2.40e-32	111
Msa1243410	MtrunA17_Chr8g0354191	69.767	43	9	1	20	62	90	128	5.61e-11	56.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 31 sgRNAs with CRISPR-Local

Find 1498 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATTGAGCAGGATATG+TGG	0.570793	8_2:+29549494	None:intergenic
AAGTCTAACTCAGACTGTGT+AGG	0.549935	8_2:+29549558	None:intergenic
AATCGACTTGTGTGCACAAG+AGG	0.532290	8_2:-29549520	Msa1243410:CDS
AGAAATGTTGAGCCTATCAA+TGG	0.616121	8_2:-29527106	Msa1243410:CDS
AGGAAGACCATCACCCATGT+TGG	0.561013	8_2:-29549608	Msa1243410:CDS
AGTCTAACTCAGACTGTGTA+GGG	0.531098	8_2:+29549559	None:intergenic
ATCAATGGTGATGGTCGTGA+TGG	0.614792	8_2:-29527091	Msa1243410:CDS
ATTGCGCCTACCTCAGCATG+AGG	0.659758	8_2:-29549628	Msa1243410:CDS
CCCATGTTGGAAGTTTAACA+AGG	0.541094	8_2:-29549595	Msa1243410:CDS
CCTCCTAGAGGAAGCTGAAG+AGG	0.517419	8_2:-29549457	Msa1243410:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAATTAGAGATCATACTCAT+TGG	-	chr8_2:29527358-29527377	Msa1243410:intron	30.0%
ACTAAGCTATAGTATTGCAA+CGG	+	chr8_2:29527694-29527713	None:intergenic	30.0%
TTATCAGAATCTATATGCAG+CGG	-	chr8_2:29527866-29527885	Msa1243410:intron	30.0%
AACACTTGATATACAATCAG+AGG	-	chr8_2:29528201-29528220	Msa1243410:intron	30.0%
TCTTTCACACCTCTATATAA+AGG	+	chr8_2:29528397-29528416	None:intergenic	30.0%
CAAATCAGGAAAAAGATCAT+TGG	+	chr8_2:29528475-29528494	None:intergenic	30.0%
TGAAGCTTAAATGATTCTGA+AGG	+	chr8_2:29529048-29529067	None:intergenic	30.0%
TGTTTGTCATCATCAAAAAG+GGG	+	chr8_2:29529243-29529262	None:intergenic	30.0%
ATTAACCTGGATAGAACTTA+GGG	+	chr8_2:29529412-29529431	None:intergenic	30.0%
AATTAACCTGGATAGAACTT+AGG	+	chr8_2:29529413-29529432	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	29527072	29549992	29527072	ID=Msa1243410;Name=Msa1243410
chr8_2	mRNA	29527072	29549992	29527072	ID=Msa1243410-mRNA-1;Parent=Msa1243410;Name=Msa1243410-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0\|0\|0\|0.4\|1\|1\|5\|0\|145
chr8_2	exon	29549986	29549992	29549986	ID=Msa1243410-mRNA-1:exon:10038;Parent=Msa1243410-mRNA-1
chr8_2	exon	29549458	29549651	29549458	ID=Msa1243410-mRNA-1:exon:10037;Parent=Msa1243410-mRNA-1
chr8_2	exon	29536438	29536443	29536438	ID=Msa1243410-mRNA-1:exon:10036;Parent=Msa1243410-mRNA-1
chr8_2	exon	29527575	29527581	29527575	ID=Msa1243410-mRNA-1:exon:10035;Parent=Msa1243410-mRNA-1
chr8_2	exon	29527072	29527295	29527072	ID=Msa1243410-mRNA-1:exon:10034;Parent=Msa1243410-mRNA-1
chr8_2	CDS	29549986	29549992	29549986	ID=Msa1243410-mRNA-1:cds;Parent=Msa1243410-mRNA-1
chr8_2	CDS	29549458	29549651	29549458	ID=Msa1243410-mRNA-1:cds;Parent=Msa1243410-mRNA-1
chr8_2	CDS	29536438	29536443	29536438	ID=Msa1243410-mRNA-1:cds;Parent=Msa1243410-mRNA-1
chr8_2	CDS	29527575	29527581	29527575	ID=Msa1243410-mRNA-1:cds;Parent=Msa1243410-mRNA-1
chr8_2	CDS	29527072	29527295	29527072	ID=Msa1243410-mRNA-1:cds;Parent=Msa1243410-mRNA-1

Gene Sequence

>Msa1243410

ATGTCGAGATTGCGCCTACCTCAGCATGAGGAAGACCATCACCCATGTTGGAAGTTTAACAAGGATTTGAGCTATAGCCCTACACAGTCTGAGTTAGACTTAGAATTAGCAATGATAATCGACTTGTGTGCACAAGAGGCAGCCACATATCCTGCTCAATCTTTTTCTGAAGTGGAAGCCCTCCTAGAGGAAGCTGAAGAGTGGCTAAGACTCGGCTACTCTCTTGATAGTTGTCGTGAGTTTTTACCTGCTGGAGCCGAAGCTACCACCGATTTTTTTAAATGTGCTGCCAGTGAATGTCACAGAAACATCTATTGTATTGTACCTGCAACTACTCTTGTCATGCCTTCAAGGGAGGATTTTGTAGAATATGTTTATGTTAGAAATGTTGAGCCTATCAATGGTGATGGTCGTGATGGTGCTGGAGAAGGACGTTGA

Protein sequence

>Msa1243410

MSRLRLPQHEEDHHPCWKFNKDLSYSPTQSELDLELAMIIDLCAQEAATYPAQSFSEVEALLEEAEEWLRLGYSLDSCREFLPAGAEATTDFFKCAASECHRNIYCIVPATTLVMPSREDFVEYVYVRNVEPINGDGRDGAGEGR*