Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1248140 | XP_013445809.1 | 73.563 | 87 | 16 | 1 | 1 | 87 | 197 | 276 | 2.83e-31 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1248140 | sp|O48686|SNL3_ARATH | 49.315 | 73 | 29 | 2 | 1 | 73 | 289 | 353 | 5.94e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1248140 | A0A072U246 | 73.563 | 87 | 16 | 1 | 1 | 87 | 197 | 276 | 1.35e-31 | 122 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1248140 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1248140 | MtrunA17_Chr8g0362641 | 73.563 | 87 | 16 | 1 | 1 | 87 | 197 | 276 | 2.60e-35 | 122 |
| Msa1248140 | MtrunA17_Chr8g0362641 | 45.833 | 72 | 32 | 1 | 1 | 72 | 116 | 180 | 8.55e-11 | 57.8 |
| Msa1248140 | MtrunA17_Chr8g0364231 | 51.111 | 90 | 37 | 1 | 3 | 92 | 341 | 423 | 2.23e-22 | 91.3 |
| Msa1248140 | MtrunA17_Chr5g0415031 | 56.164 | 73 | 27 | 1 | 1 | 73 | 325 | 392 | 3.35e-19 | 82.0 |
| Msa1248140 | MtrunA17_Chr6g0452001 | 51.429 | 70 | 27 | 1 | 3 | 72 | 347 | 409 | 4.81e-14 | 67.4 |
| Msa1248140 | MtrunA17_Chr6g0452021 | 45.946 | 74 | 33 | 1 | 3 | 76 | 280 | 346 | 9.05e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1248140 | AT1G24190.1 | 49.315 | 73 | 29 | 2 | 1 | 73 | 289 | 353 | 6.04e-13 | 64.7 |
| Msa1248140 | AT1G24190.3 | 49.315 | 73 | 29 | 2 | 1 | 73 | 289 | 353 | 6.16e-13 | 64.7 |
| Msa1248140 | AT1G24190.2 | 49.315 | 73 | 29 | 2 | 1 | 73 | 289 | 353 | 6.34e-13 | 64.7 |
| Msa1248140 | AT1G70060.2 | 46.053 | 76 | 33 | 2 | 1 | 76 | 305 | 372 | 3.69e-12 | 62.4 |
| Msa1248140 | AT1G70060.1 | 46.053 | 76 | 33 | 2 | 1 | 76 | 305 | 372 | 3.69e-12 | 62.4 |
| Msa1248140 | AT3G01320.1 | 50.000 | 68 | 27 | 1 | 5 | 72 | 341 | 401 | 6.49e-12 | 61.6 |
| Msa1248140 | AT3G01320.2 | 50.000 | 68 | 27 | 1 | 5 | 72 | 329 | 389 | 6.88e-12 | 61.6 |
| Msa1248140 | AT5G15020.1 | 48.529 | 68 | 28 | 1 | 5 | 72 | 337 | 397 | 1.60e-11 | 60.5 |
| Msa1248140 | AT5G15020.2 | 48.529 | 68 | 28 | 1 | 5 | 72 | 337 | 397 | 1.71e-11 | 60.5 |
Find 18 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAGAGGTGGATGATTTAT+TGG | 0.164277 | 8_2:-41676859 | Msa1248140:CDS |
| CCGAGAGGAATTACAATTAT+TGG | 0.178616 | 8_2:-41677306 | Msa1248140:intron |
| CTTCAAGGCTTTGACAATTT+CGG | 0.267451 | 8_2:+41676773 | None:intergenic |
| GGGAAAATATACTGATCTTA+TGG | 0.325614 | 8_2:-41676838 | Msa1248140:CDS |
| CAAGAGGTGGATGATTTATT+GGG | 0.341307 | 8_2:-41676858 | Msa1248140:CDS |
| CTCTCGGGTAATTATTTCTC+TGG | 0.364552 | 8_2:+41677322 | None:intergenic |
| AAAGATTTGATGACCCTTCA+AGG | 0.381143 | 8_2:+41676758 | None:intergenic |
| CCAATAAATCATCCACCTCT+TGG | 0.383684 | 8_2:+41676859 | None:intergenic |
| CGAAATTGTCAAAGCCTTGA+AGG | 0.474140 | 8_2:-41676772 | Msa1248140:CDS |
| TTTCTCTGGCACAGATATGT+AGG | 0.478650 | 8_2:+41677336 | None:intergenic |
| CCAATAATTGTAATTCCTCT+CGG | 0.508805 | 8_2:+41677306 | None:intergenic |
| ACAAAAGTATAATGAGAAGA+TGG | 0.512117 | 8_2:+41676653 | None:intergenic |
| CAATAATTGTAATTCCTCTC+GGG | 0.513671 | 8_2:+41677307 | None:intergenic |
| AGTATAATGAGAAGATGGCT+TGG | 0.521787 | 8_2:+41676658 | None:intergenic |
| TTATTTATATTTCCAAGAGG+TGG | 0.542195 | 8_2:-41676871 | Msa1248140:CDS |
| GAAATTGTCAAAGCCTTGAA+GGG | 0.600097 | 8_2:-41676771 | Msa1248140:CDS |
| GCTTTATTTATATTTCCAAG+AGG | 0.675688 | 8_2:-41676874 | Msa1248140:CDS |
| CAGAGAAATAATTACCCGAG+AGG | 0.689632 | 8_2:-41677321 | Msa1248140:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAAATCTTGATAATTTTC+AGG | + | chr8_2:41676689-41676708 | None:intergenic | 15.0% |
| !!! | CTAACCTTTTAATTAAAAAA+AGG | - | chr8_2:41676879-41676898 | Msa1248140:intron | 15.0% |
| !!! | GTAACCTTTTTTTAATTAAA+AGG | + | chr8_2:41676886-41676905 | None:intergenic | 15.0% |
| !! | TTAATTAAAAAAAGGTTACA+TGG | - | chr8_2:41676887-41676906 | Msa1248140:intron | 15.0% |
| !!! | AAAAAATCTTGATAATTTTC+AGG | + | chr8_2:41676689-41676708 | None:intergenic | 15.0% |
| !!! | CTAACCTTTTAATTAAAAAA+AGG | - | chr8_2:41676879-41676898 | Msa1248140:intron | 15.0% |
| !!! | GTAACCTTTTTTTAATTAAA+AGG | + | chr8_2:41676886-41676905 | None:intergenic | 15.0% |
| !! | TTAATTAAAAAAAGGTTACA+TGG | - | chr8_2:41676887-41676906 | Msa1248140:intron | 15.0% |
| !!! | TAGTACTCTTTAACTTAATT+TGG | - | chr8_2:41676838-41676857 | Msa1248140:CDS | 20.0% |
| !!! | TAGTACTCTTTAACTTAATT+TGG | - | chr8_2:41676838-41676857 | Msa1248140:CDS | 20.0% |
| !!! | TAAAGAGTACTAATACGATA+TGG | + | chr8_2:41676830-41676849 | None:intergenic | 25.0% |
| ! | ATTAAAAGGTTAGTGTTCAA+TGG | + | chr8_2:41676872-41676891 | None:intergenic | 25.0% |
| ! | GGAATCGAAGAATAAAATAT+TGG | - | chr8_2:41676913-41676932 | Msa1248140:intron | 25.0% |
| !! | TATATTTTGTTCAAGAACTG+AGG | - | chr8_2:41677051-41677070 | Msa1248140:intron | 25.0% |
| ! | TCTAAGTAGTTGCTAATTTA+TGG | - | chr8_2:41677152-41677171 | Msa1248140:intron | 25.0% |
| !!! | GCTTTATTTATATTTCCAAG+AGG | - | chr8_2:41677177-41677196 | Msa1248140:intron | 25.0% |
| ! | TTATTTATATTTCCAAGAGG+TGG | - | chr8_2:41677180-41677199 | Msa1248140:intron | 25.0% |
| !! | ACAAAAGTATAATGAGAAGA+TGG | + | chr8_2:41677401-41677420 | None:intergenic | 25.0% |
| !!! | TAAAGAGTACTAATACGATA+TGG | + | chr8_2:41676830-41676849 | None:intergenic | 25.0% |
| ! | ATTAAAAGGTTAGTGTTCAA+TGG | + | chr8_2:41676872-41676891 | None:intergenic | 25.0% |
| ! | GGAATCGAAGAATAAAATAT+TGG | - | chr8_2:41676913-41676932 | Msa1248140:intron | 25.0% |
| !! | TATATTTTGTTCAAGAACTG+AGG | - | chr8_2:41677051-41677070 | Msa1248140:intron | 25.0% |
| ! | TCTAAGTAGTTGCTAATTTA+TGG | - | chr8_2:41677152-41677171 | Msa1248140:intron | 25.0% |
| !!! | GCTTTATTTATATTTCCAAG+AGG | - | chr8_2:41677177-41677196 | Msa1248140:intron | 25.0% |
| ! | TTATTTATATTTCCAAGAGG+TGG | - | chr8_2:41677180-41677199 | Msa1248140:intron | 25.0% |
| !! | ACAAAAGTATAATGAGAAGA+TGG | + | chr8_2:41677401-41677420 | None:intergenic | 25.0% |
| CAATAATTGTAATTCCTCTC+GGG | + | chr8_2:41676747-41676766 | None:intergenic | 30.0% | |
| CCAATAATTGTAATTCCTCT+CGG | + | chr8_2:41676748-41676767 | None:intergenic | 30.0% | |
| ! | CTTTTATGTATCCAAATTCG+TGG | - | chr8_2:41676779-41676798 | Msa1248140:CDS | 30.0% |
| TAAAAAAAGGTTACATGGCA+AGG | - | chr8_2:41676892-41676911 | Msa1248140:intron | 30.0% | |
| AATATAAACTACCCTTCATG+AGG | - | chr8_2:41676973-41676992 | Msa1248140:intron | 30.0% | |
| ! | ACCTGAAGAAAAAAAAGTGT+TGG | - | chr8_2:41676999-41677018 | Msa1248140:intron | 30.0% |
| !!! | TCCAACACTTTTTTTTCTTC+AGG | + | chr8_2:41677003-41677022 | None:intergenic | 30.0% |
| GGGAAAATATACTGATCTTA+TGG | - | chr8_2:41677213-41677232 | Msa1248140:intron | 30.0% | |
| CAATAATTGTAATTCCTCTC+GGG | + | chr8_2:41676747-41676766 | None:intergenic | 30.0% | |
| CCAATAATTGTAATTCCTCT+CGG | + | chr8_2:41676748-41676767 | None:intergenic | 30.0% | |
| ! | CTTTTATGTATCCAAATTCG+TGG | - | chr8_2:41676779-41676798 | Msa1248140:CDS | 30.0% |
| TAAAAAAAGGTTACATGGCA+AGG | - | chr8_2:41676892-41676911 | Msa1248140:intron | 30.0% | |
| AATATAAACTACCCTTCATG+AGG | - | chr8_2:41676973-41676992 | Msa1248140:intron | 30.0% | |
| ! | ACCTGAAGAAAAAAAAGTGT+TGG | - | chr8_2:41676999-41677018 | Msa1248140:intron | 30.0% |
| !!! | TCCAACACTTTTTTTTCTTC+AGG | + | chr8_2:41677003-41677022 | None:intergenic | 30.0% |
| GGGAAAATATACTGATCTTA+TGG | - | chr8_2:41677213-41677232 | Msa1248140:intron | 30.0% | |
| !!! | CTCTTTGACTTTTTCAACGA+AGG | + | chr8_2:41676654-41676673 | None:intergenic | 35.0% |
| CCGAGAGGAATTACAATTAT+TGG | - | chr8_2:41676745-41676764 | Msa1248140:CDS | 35.0% | |
| GTTTACACTCCATTCAGTTT+AGG | - | chr8_2:41677073-41677092 | Msa1248140:intron | 35.0% | |
| CAATCTGATCCTAAACTGAA+TGG | + | chr8_2:41677085-41677104 | None:intergenic | 35.0% | |
| CAAGAGGTGGATGATTTATT+GGG | - | chr8_2:41677193-41677212 | Msa1248140:intron | 35.0% | |
| ! | CTTCAAGGCTTTGACAATTT+CGG | + | chr8_2:41677281-41677300 | None:intergenic | 35.0% |
| GAAATTGTCAAAGCCTTGAA+GGG | - | chr8_2:41677280-41677299 | Msa1248140:intron | 35.0% | |
| ! | AAAGATTTGATGACCCTTCA+AGG | + | chr8_2:41677296-41677315 | None:intergenic | 35.0% |
| AGTATAATGAGAAGATGGCT+TGG | + | chr8_2:41677396-41677415 | None:intergenic | 35.0% | |
| !!! | CTCTTTGACTTTTTCAACGA+AGG | + | chr8_2:41676654-41676673 | None:intergenic | 35.0% |
| CCGAGAGGAATTACAATTAT+TGG | - | chr8_2:41676745-41676764 | Msa1248140:CDS | 35.0% | |
| GTTTACACTCCATTCAGTTT+AGG | - | chr8_2:41677073-41677092 | Msa1248140:intron | 35.0% | |
| CAATCTGATCCTAAACTGAA+TGG | + | chr8_2:41677085-41677104 | None:intergenic | 35.0% | |
| CAAGAGGTGGATGATTTATT+GGG | - | chr8_2:41677193-41677212 | Msa1248140:intron | 35.0% | |
| ! | CTTCAAGGCTTTGACAATTT+CGG | + | chr8_2:41677281-41677300 | None:intergenic | 35.0% |
| GAAATTGTCAAAGCCTTGAA+GGG | - | chr8_2:41677280-41677299 | Msa1248140:intron | 35.0% | |
| ! | AAAGATTTGATGACCCTTCA+AGG | + | chr8_2:41677296-41677315 | None:intergenic | 35.0% |
| AGTATAATGAGAAGATGGCT+TGG | + | chr8_2:41677396-41677415 | None:intergenic | 35.0% | |
| TTTCTCTGGCACAGATATGT+AGG | + | chr8_2:41676718-41676737 | None:intergenic | 40.0% | |
| ! | CTCTCGGGTAATTATTTCTC+TGG | + | chr8_2:41676732-41676751 | None:intergenic | 40.0% |
| CAGAGAAATAATTACCCGAG+AGG | - | chr8_2:41676730-41676749 | Msa1248140:CDS | 40.0% | |
| AAATGCAGCATCCACGAATT+TGG | + | chr8_2:41676793-41676812 | None:intergenic | 40.0% | |
| !! | CTTCAGGTTTTCCTCATGAA+GGG | + | chr8_2:41676987-41677006 | None:intergenic | 40.0% |
| !! | TCTTCAGGTTTTCCTCATGA+AGG | + | chr8_2:41676988-41677007 | None:intergenic | 40.0% |
| CCAATAAATCATCCACCTCT+TGG | + | chr8_2:41677195-41677214 | None:intergenic | 40.0% | |
| CCAAGAGGTGGATGATTTAT+TGG | - | chr8_2:41677192-41677211 | Msa1248140:intron | 40.0% | |
| CGAAATTGTCAAAGCCTTGA+AGG | - | chr8_2:41677279-41677298 | Msa1248140:intron | 40.0% | |
| TTTCTCTGGCACAGATATGT+AGG | + | chr8_2:41676718-41676737 | None:intergenic | 40.0% | |
| ! | CTCTCGGGTAATTATTTCTC+TGG | + | chr8_2:41676732-41676751 | None:intergenic | 40.0% |
| CAGAGAAATAATTACCCGAG+AGG | - | chr8_2:41676730-41676749 | Msa1248140:CDS | 40.0% | |
| AAATGCAGCATCCACGAATT+TGG | + | chr8_2:41676793-41676812 | None:intergenic | 40.0% | |
| !! | CTTCAGGTTTTCCTCATGAA+GGG | + | chr8_2:41676987-41677006 | None:intergenic | 40.0% |
| !! | TCTTCAGGTTTTCCTCATGA+AGG | + | chr8_2:41676988-41677007 | None:intergenic | 40.0% |
| CCAATAAATCATCCACCTCT+TGG | + | chr8_2:41677195-41677214 | None:intergenic | 40.0% | |
| CCAAGAGGTGGATGATTTAT+TGG | - | chr8_2:41677192-41677211 | Msa1248140:intron | 40.0% | |
| CGAAATTGTCAAAGCCTTGA+AGG | - | chr8_2:41677279-41677298 | Msa1248140:intron | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 41676641 | 41677432 | 41676641 | ID=Msa1248140;Name=Msa1248140 |
| chr8_2 | mRNA | 41676641 | 41677432 | 41676641 | ID=Msa1248140-mRNA-1;Parent=Msa1248140;Name=Msa1248140-mRNA-1;_AED=0.60;_eAED=0.60;_QI=0|0|0|1|0|0|2|0|127 |
| chr8_2 | exon | 41677307 | 41677432 | 41677307 | ID=Msa1248140-mRNA-1:exon:12706;Parent=Msa1248140-mRNA-1 |
| chr8_2 | exon | 41676641 | 41676895 | 41676641 | ID=Msa1248140-mRNA-1:exon:12705;Parent=Msa1248140-mRNA-1 |
| chr8_2 | CDS | 41677307 | 41677432 | 41677307 | ID=Msa1248140-mRNA-1:cds;Parent=Msa1248140-mRNA-1 |
| chr8_2 | CDS | 41676641 | 41676895 | 41676641 | ID=Msa1248140-mRNA-1:cds;Parent=Msa1248140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
