AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1248820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1248820 MtrunA17_Chr8g0363921 96.140 285 4 2 1 285 1 278 0.0 551
Msa1248820 MtrunA17_Chr5g0415291 69.585 217 46 6 75 285 37 239 2.01e-95 281
Msa1248820 MtrunA17_Chr7g0234761 53.171 205 74 6 84 276 234 428 6.10e-52 177
Msa1248820 MtrunA17_Chr8g0339091 63.309 139 40 3 109 239 237 372 7.80e-51 174
Msa1248820 MtrunA17_Chr5g0421111 71.930 114 30 1 134 247 343 454 2.40e-47 166
Msa1248820 MtrunA17_Chr8g0365941 69.298 114 33 1 134 247 283 394 2.09e-46 162
Msa1248820 MtrunA17_Chr1g0152131 39.865 296 125 8 5 257 74 359 5.51e-46 160
Msa1248820 MtrunA17_Chr4g0035101 55.484 155 53 2 110 251 85 236 4.55e-45 155
Msa1248820 MtrunA17_Chr3g0144641 60.417 144 44 4 123 254 150 292 6.30e-45 156
Msa1248820 MtrunA17_Chr8g0385911 88.000 75 9 0 155 229 142 216 3.61e-40 142
Msa1248820 MtrunA17_Chr3g0131771 82.716 81 14 0 149 229 138 218 8.52e-40 141
Msa1248820 MtrunA17_Chr7g0258161 72.093 86 24 0 152 237 167 252 1.49e-37 135
Msa1248820 MtrunA17_Chr1g0174161 69.412 85 26 0 155 239 157 241 4.43e-36 131
Msa1248820 MtrunA17_Chr6g0484271 49.640 139 53 3 100 238 114 235 5.87e-33 122
Msa1248820 MtrunA17_Chr4g0074161 59.756 82 32 1 142 223 190 270 4.26e-22 95.1
Msa1248820 MtrunA17_Chr1g0190731 58.974 78 26 2 146 223 186 257 6.68e-20 88.2
Msa1248820 MtrunA17_Chr1g0182791 47.561 82 41 2 160 241 319 398 3.12e-15 75.5
Msa1248820 MtrunA17_Chr1g0193321 50.000 64 31 1 160 223 464 526 4.27e-15 75.5
Msa1248820 MtrunA17_Chr2g0329041 38.194 144 72 5 88 220 257 394 7.41e-15 73.9
Msa1248820 MtrunA17_Chr4g0032951 55.385 65 29 0 158 222 355 419 1.48e-14 73.2
Msa1248820 MtrunA17_Chr1g0174271 48.101 79 36 1 146 224 291 364 1.34e-13 70.5
Msa1248820 MtrunA17_Chr4g0014771 49.231 65 33 0 158 222 118 182 3.26e-13 67.0
Msa1248820 MtrunA17_Chr7g0258181 44.595 74 41 0 158 231 243 316 4.52e-13 68.2
Msa1248820 MtrunA17_Chr1g0184841 60.606 66 25 1 158 223 120 184 4.72e-13 67.8
Msa1248820 MtrunA17_Chr1g0152651 49.231 65 32 1 158 222 274 337 1.83e-12 67.0
Msa1248820 MtrunA17_Chr1g0153241 36.364 110 50 4 115 224 61 150 3.56e-12 65.1
Msa1248820 MtrunA17_Chr3g0134741 35.659 129 69 3 107 222 165 292 4.83e-12 65.5
Msa1248820 MtrunA17_Chr8g0351881 47.692 65 34 0 158 222 316 380 7.71e-12 65.1
Msa1248820 MtrunA17_Chr5g0393721 50.000 64 31 1 159 222 225 287 9.83e-12 64.3
Msa1248820 MtrunA17_Chr7g0262691 46.875 64 33 1 160 223 254 316 1.30e-11 64.7
Msa1248820 MtrunA17_Chr7g0271251 48.438 64 32 1 160 223 434 496 1.74e-11 64.3
Msa1248820 MtrunA17_Chr3g0095391 46.875 64 33 1 160 223 311 373 2.72e-11 63.5
Msa1248820 MtrunA17_Chr5g0402031 50.000 56 27 1 169 224 116 170 9.48e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1248820 AT1G59640.1 58.020 293 86 11 1 285 1 264 7.63e-96 283
Msa1248820 AT1G59640.2 57.600 250 70 11 1 243 1 221 1.13e-74 232
Msa1248820 AT5G62610.2 51.525 295 119 12 1 285 1 281 7.11e-71 220
Msa1248820 AT5G62610.1 51.525 295 119 12 1 285 1 281 7.11e-71 220
Msa1248820 AT3G07340.2 57.500 160 46 3 84 243 206 343 1.18e-47 165
Msa1248820 AT3G07340.1 57.500 160 46 3 84 243 206 343 2.24e-47 164
Msa1248820 AT1G26260.3 55.696 158 64 2 92 248 110 262 5.81e-46 158
Msa1248820 AT5G48560.2 77.451 102 19 3 143 243 289 387 7.39e-46 160
Msa1248820 AT5G48560.1 77.451 102 19 3 143 243 289 387 1.24e-45 160
Msa1248820 AT1G26260.5 55.696 158 64 2 92 248 139 291 1.25e-45 158
Msa1248820 AT1G26260.4 55.696 158 64 2 92 248 161 313 2.28e-45 157
Msa1248820 AT1G26260.1 55.696 158 64 2 92 248 161 313 2.28e-45 157
Msa1248820 AT1G26260.6 55.696 158 64 2 92 248 161 313 2.28e-45 157
Msa1248820 AT1G26260.2 55.696 158 64 2 92 248 161 313 2.28e-45 157
Msa1248820 AT1G68920.3 75.758 99 24 0 148 246 292 390 6.30e-45 158
Msa1248820 AT1G68920.1 75.758 99 24 0 148 246 293 391 6.40e-45 158
Msa1248820 AT1G68920.4 75.758 99 24 0 148 246 293 391 6.40e-45 158
Msa1248820 AT1G68920.2 75.758 99 24 0 148 246 293 391 6.40e-45 158
Msa1248820 AT5G50915.3 56.115 139 49 3 93 229 79 207 8.37e-44 150
Msa1248820 AT5G50915.4 56.115 139 49 3 93 229 79 207 8.37e-44 150
Msa1248820 AT3G23690.1 61.029 136 42 3 97 232 141 265 1.45e-43 152
Msa1248820 AT5G50915.1 56.522 138 48 3 93 228 79 206 1.92e-43 150
Msa1248820 AT5G50915.2 56.522 138 48 3 93 228 79 206 1.92e-43 150
Msa1248820 AT1G10120.2 70.833 96 28 0 151 246 199 294 2.76e-42 149
Msa1248820 AT1G10120.1 70.833 96 28 0 151 246 199 294 2.76e-42 149
Msa1248820 AT4G36540.1 52.740 146 67 1 101 244 82 227 8.13e-42 146
Msa1248820 AT4G36540.2 53.472 144 65 1 101 242 82 225 2.57e-41 145
Msa1248820 AT2G18300.2 70.103 97 29 0 148 244 175 271 7.85e-41 144
Msa1248820 AT4G34530.1 82.500 80 14 0 152 231 166 245 1.48e-40 144
Msa1248820 AT2G18300.3 70.103 97 29 0 148 244 175 271 1.61e-40 144
Msa1248820 AT2G18300.1 71.579 95 27 0 148 242 175 269 2.23e-40 143
Msa1248820 AT2G42300.4 69.318 88 27 0 152 239 179 266 2.75e-38 136
Msa1248820 AT3G57800.2 71.591 88 25 0 152 239 198 285 8.94e-38 137
Msa1248820 AT2G42300.1 65.957 94 32 0 152 245 179 272 1.38e-37 135
Msa1248820 AT1G25330.1 48.507 134 60 2 115 246 66 192 4.13e-34 124
Msa1248820 AT1G18400.2 74.667 75 19 0 156 230 143 217 8.34e-34 124
Msa1248820 AT1G18400.1 74.667 75 19 0 156 230 143 217 1.56e-33 123
Msa1248820 AT1G73830.1 69.512 82 25 0 157 238 146 227 2.62e-33 122
Msa1248820 AT1G73830.2 69.512 82 25 0 157 238 146 227 2.79e-33 122
Msa1248820 AT3G57800.1 46.667 135 25 1 152 239 198 332 6.00e-29 114
Msa1248820 AT2G24260.2 63.014 73 26 1 151 223 28 99 1.66e-22 94.0
Msa1248820 AT2G24260.1 63.014 73 26 1 151 223 131 202 1.26e-21 93.6
Msa1248820 AT4G30980.1 68.182 66 20 1 158 223 130 194 5.84e-21 90.9
Msa1248820 AT5G58010.1 61.842 76 28 1 148 223 89 163 7.42e-21 90.5
Msa1248820 AT4G30980.2 68.182 66 20 1 158 223 178 242 7.45e-21 91.3
Msa1248820 AT2G42300.5 78.723 47 10 0 152 198 179 225 2.74e-19 84.7
Msa1248820 AT2G42300.3 78.723 47 10 0 152 198 179 225 2.74e-19 84.7
Msa1248820 AT2G42300.2 80.435 46 9 0 152 197 179 224 3.59e-19 84.7
Msa1248820 AT4G02590.3 49.515 103 46 3 126 223 46 147 1.25e-15 75.1
Msa1248820 AT2G20180.7 32.317 164 97 5 61 223 121 271 1.96e-15 76.3
Msa1248820 AT2G20180.1 32.317 164 97 5 61 223 121 271 1.96e-15 76.3
Msa1248820 AT2G20180.5 32.317 164 97 5 61 223 121 271 1.96e-15 76.3
Msa1248820 AT4G02590.2 49.515 103 46 3 126 223 109 210 2.18e-15 75.5
Msa1248820 AT4G02590.1 49.515 103 46 3 126 223 109 210 2.18e-15 75.5
Msa1248820 AT2G20180.4 32.317 164 97 5 61 223 192 342 3.21e-15 75.9
Msa1248820 AT2G20180.6 32.317 164 97 5 61 223 192 342 3.21e-15 75.9
Msa1248820 AT2G20180.2 32.317 164 97 5 61 223 192 342 3.21e-15 75.9
Msa1248820 AT2G20180.3 32.317 164 97 5 61 223 192 342 3.21e-15 75.9
Msa1248820 AT1G09530.3 50.000 64 31 1 160 223 339 401 9.04e-15 74.7
Msa1248820 AT1G09530.2 50.000 64 31 1 160 223 339 401 9.04e-15 74.7
Msa1248820 AT1G09530.5 50.000 64 31 1 160 223 339 401 9.04e-15 74.7
Msa1248820 AT1G09530.1 50.000 64 31 1 160 223 339 401 9.04e-15 74.7
Msa1248820 AT1G09530.4 50.000 64 31 1 160 223 339 401 9.04e-15 74.7
Msa1248820 AT1G09530.6 50.000 64 31 1 160 223 339 401 9.04e-15 74.7
Msa1248820 AT1G03040.1 48.544 103 47 3 126 223 107 208 2.21e-14 72.4
Msa1248820 AT1G03040.3 48.544 103 47 3 126 223 107 208 2.33e-14 72.8
Msa1248820 AT4G28800.1 44.000 75 41 1 161 235 252 325 1.46e-13 70.9
Msa1248820 AT1G03040.2 54.217 83 35 2 143 223 122 203 2.28e-13 69.3
Msa1248820 AT2G20180.8 31.677 161 96 5 61 220 192 339 2.97e-13 69.3
Msa1248820 AT2G42280.1 50.000 64 32 0 158 221 279 342 4.14e-13 69.3
Msa1248820 AT1G51140.1 53.125 64 30 0 158 221 304 367 4.16e-13 69.3
Msa1248820 AT4G28815.2 51.724 58 27 1 161 218 71 127 4.68e-13 67.8
Msa1248820 AT4G09180.1 46.269 67 36 0 156 222 182 248 7.33e-13 67.4
Msa1248820 AT2G42280.3 51.515 66 30 1 158 221 279 344 8.33e-13 68.2
Msa1248820 AT4G28800.2 45.833 72 37 2 161 231 264 334 1.06e-12 68.2
Msa1248820 AT4G28815.1 51.724 58 27 1 161 218 147 203 1.56e-12 67.0
Msa1248820 AT4G28790.1 35.200 125 61 5 160 280 273 381 1.56e-12 67.8
Msa1248820 AT2G46970.1 43.836 73 35 2 156 223 216 287 2.22e-12 67.4
Msa1248820 AT2G46970.2 43.836 73 35 2 156 223 185 256 2.43e-12 67.0
Msa1248820 AT2G46970.3 43.836 73 35 2 156 223 194 265 2.67e-12 67.0
Msa1248820 AT4G33880.1 42.857 84 45 2 141 222 247 329 4.07e-12 66.2
Msa1248820 AT1G35460.1 46.154 65 35 0 158 222 181 245 5.19e-12 65.1
Msa1248820 AT4G00050.2 47.692 65 33 1 158 222 105 168 1.29e-11 64.3
Msa1248820 AT1G27740.1 49.231 65 32 1 158 222 167 230 2.03e-11 63.2
Msa1248820 AT2G14760.1 49.231 65 32 1 158 222 237 300 2.08e-11 63.9
Msa1248820 AT4G00050.3 47.692 65 33 1 158 222 207 270 2.64e-11 63.9
Msa1248820 AT4G36930.1 47.541 61 31 1 164 224 197 256 8.27e-11 62.4

Find 116 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTTCAATTTAGGTTAT+TGG 0.179282 8_2:+43046959 Msa1248820:intron
GTGTACTATAGTGGACTTTC+AGG 0.196499 8_2:+43048140 Msa1248820:three_prime_UTR
CCATACCTTTGTGTAGAAAA+AGG 0.221508 8_2:-43048044 None:intergenic
TGTTTAACACTTACATCTTT+TGG 0.232272 8_2:-43047214 None:intergenic
TTTCTTCAATTTAGGTTATT+GGG 0.258352 8_2:+43046960 Msa1248820:intron
CAGTTTCCAGCGGCGATTAA+TGG 0.284846 8_2:+43045190 Msa1248820:CDS
TTTAGTTCCTGGTTGTAATA+AGG 0.288451 8_2:+43046719 Msa1248820:CDS
CCGCAGTTTGGGAACGGTTT+AGG 0.291510 8_2:+43045241 Msa1248820:CDS
AGTTGAAAACGGTTCGGTTC+GGG 0.306795 8_2:+43045063 Msa1248820:five_prime_UTR
CAACAGGCTGCTTCAGTTTG+TGG 0.325216 8_2:+43048082 Msa1248820:three_prime_UTR
GACTAAGAAGGCCGCAGTTT+GGG 0.332351 8_2:+43045230 Msa1248820:CDS
CTCAATATTGTGTACTATAG+TGG 0.335669 8_2:+43048131 Msa1248820:three_prime_UTR
ATTCTACAGGATTTAGTTCC+TGG 0.338943 8_2:+43046708 Msa1248820:CDS
TTAGGACAGTTTGGTGAGTT+TGG 0.345423 8_2:+43045259 Msa1248820:CDS
AGAGAGAGAGAAAGAGTGTT+AGG 0.354588 8_2:-43044940 None:intergenic
GAGTTGAAAACGGTTCGGTT+CGG 0.365096 8_2:+43045062 Msa1248820:five_prime_UTR
CATCGCTCCTGGATCCATTT+CGG 0.370295 8_2:-43045101 None:intergenic
CGTTTAGTTTAGCGGAGATC+TGG 0.375505 8_2:+43045167 Msa1248820:CDS
CAACAAACGTTTGATCTTGC+CGG 0.377074 8_2:+43047783 Msa1248820:CDS
AATGGCGGCGGAGGTGGATT+GGG 0.382699 8_2:+43045208 Msa1248820:CDS
ATTACCATCCCGGTTTAAAC+CGG 0.385440 8_2:-43045284 None:intergenic
ACCTGCATATGTAACCATTC+TGG 0.392490 8_2:-43047855 None:intergenic
CCCTCGTTCATCATCGCTCC+TGG 0.400423 8_2:-43045112 None:intergenic
ACATCCATGTTCGAGCTAGA+AGG 0.403285 8_2:+43046293 Msa1248820:CDS
TGGACAATAAACAGGCTAGA+AGG 0.405648 8_2:+43046661 Msa1248820:intron
CAGATCTCCGCTAAACTAAA+CGG 0.416576 8_2:-43045166 None:intergenic
GTTGAAGGAGAAGTCAGTTC+AGG 0.418689 8_2:+43046214 Msa1248820:CDS
GGAGCATTTGAAGGATGAAA+TGG 0.421937 8_2:-43047958 None:intergenic
TAATGGCGGCGGAGGTGGAT+TGG 0.422484 8_2:+43045207 Msa1248820:CDS
TTACGCGTTCTGCAAGACTA+TGG 0.424378 8_2:-43046333 None:intergenic
GCGATTAATGGCGGCGGAGG+TGG 0.426598 8_2:+43045202 Msa1248820:CDS
AGTTTGGACCCGGTTTAAAC+CGG 0.426792 8_2:+43045275 Msa1248820:CDS
GGACTAAGAAGGCCGCAGTT+TGG 0.434537 8_2:+43045229 Msa1248820:CDS
TTGAAGTTAGTGAACCGAAA+TGG 0.445482 8_2:+43045087 Msa1248820:exon
CAGTTTGGTGAGTTTGGACC+CGG 0.446797 8_2:+43045265 Msa1248820:CDS
GGAAAACGGACTAAAGCATC+AGG 0.450766 8_2:+43046163 Msa1248820:CDS
CTTGTAGCTTGTGAAACAAA+TGG 0.451137 8_2:-43047810 None:intergenic
GGTTTAAACCGGGATGGTAA+TGG 0.452515 8_2:+43045286 Msa1248820:CDS
TACGACGCCGTTTAGTTTAG+CGG 0.455033 8_2:+43045159 Msa1248820:CDS
TGAAAGGATGAAAATTCTAC+AGG 0.461627 8_2:+43046695 Msa1248820:CDS
TAGTGAACCGAAATGGATCC+AGG 0.464407 8_2:+43045094 Msa1248820:exon
GGTTCGGTGCGTGATCTCCT+TGG 0.468651 8_2:-43044919 None:intergenic
GTCGTATTTCCATTCCCATT+CGG 0.470342 8_2:-43045142 None:intergenic
CATCCATGTTCGAGCTAGAA+GGG 0.474849 8_2:+43046294 Msa1248820:CDS
GATTAATGTTGAGTTGAAAA+CGG 0.477674 8_2:+43045052 Msa1248820:five_prime_UTR
GGTTCATTTCCGAATGGGAA+TGG 0.479304 8_2:+43045133 Msa1248820:CDS
GGGAACGGTTTAGGACAGTT+TGG 0.481836 8_2:+43045250 Msa1248820:CDS
ACCAGAATGGTTACATATGC+AGG 0.483200 8_2:+43047854 Msa1248820:CDS
TATAATATAGGAGCATTTGA+AGG 0.491142 8_2:-43047967 None:intergenic
AACCATGGTGGTGTTGGTGG+TGG 0.493213 8_2:+43045331 Msa1248820:CDS
ACATGGATGTAATCTTGCTT+AGG 0.502301 8_2:-43046280 None:intergenic
ACGAGGGTTCATTTCCGAAT+GGG 0.502721 8_2:+43045128 Msa1248820:CDS
AACAAGAATGAGGGTGGTGA+TGG 0.502949 8_2:+43046187 Msa1248820:CDS
CCGCCGCCATTAATCGCCGC+TGG 0.503828 8_2:-43045196 None:intergenic
TTGAACCATGGTGGTGTTGG+TGG 0.507286 8_2:+43045328 Msa1248820:CDS
GCGGCGATTAATGGCGGCGG+AGG 0.511591 8_2:+43045199 Msa1248820:CDS
GCAGAGGTTCTTCACCAGAA+TGG 0.515410 8_2:+43047841 Msa1248820:CDS
TCGTGTTTGTTTGTGTAGCA+AGG 0.516464 8_2:+43048280 Msa1248820:three_prime_UTR
TGGATGTAATCTTGCTTAGG+AGG 0.520256 8_2:-43046277 None:intergenic
TGGAGGAAAGGCTTCAATGC+CGG 0.520377 8_2:-43047194 None:intergenic
GAGAGAAAGAGTGTTAGGTT+CGG 0.525245 8_2:-43044935 None:intergenic
ATGTTGAGTTGAAAACGGTT+CGG 0.525360 8_2:+43045057 Msa1248820:five_prime_UTR
ACTCCGGTCCATTACCATCC+CGG 0.528802 8_2:-43045294 None:intergenic
TTAGGTTATTGGGAAAGCAT+TGG 0.529281 8_2:+43046970 Msa1248820:intron
GTTTGGACCCGGTTTAAACC+GGG 0.537559 8_2:+43045276 Msa1248820:CDS
TCTGGTGAAGAACCTCTGCT+TGG 0.539168 8_2:-43047837 None:intergenic
AATAATGAATACCACGGCAT+TGG 0.540706 8_2:-43045416 None:intergenic
GGACAATAAACAGGCTAGAA+GGG 0.544423 8_2:+43046662 Msa1248820:intron
TTACCATCCCGGTTTAAACC+GGG 0.553757 8_2:-43045283 None:intergenic
GCACCATTCTCCACTGTCCA+AGG 0.555247 8_2:+43044902 Msa1248820:five_prime_UTR
CCAGCGGCGATTAATGGCGG+CGG 0.557594 8_2:+43045196 Msa1248820:CDS
TTACATATGCAGGTAGGTGG+TGG 0.558363 8_2:+43047864 Msa1248820:CDS
AGAAGGCCGCAGTTTGGGAA+CGG 0.559428 8_2:+43045235 Msa1248820:CDS
CTTAAGGTGTTAAAGTCCAA+TGG 0.560546 8_2:+43048006 Msa1248820:three_prime_UTR
AGCATCAGGGAACAAGAATG+AGG 0.561192 8_2:+43046177 Msa1248820:CDS
GGAATGAAGAAAGTGTCCAT+TGG 0.561544 8_2:-43048022 None:intergenic
AGAATGATAGTGATGGAAAA+CGG 0.563521 8_2:+43046149 Msa1248820:intron
AACGAGGGTTCATTTCCGAA+TGG 0.564078 8_2:+43045127 Msa1248820:CDS
TTGTGAAACAAATGGCATGC+CGG 0.566973 8_2:-43047802 None:intergenic
TGCGTGATCTCCTTGGACAG+TGG 0.568345 8_2:-43044912 None:intergenic
GACCCCTTCTAGCTCGAACA+TGG 0.574237 8_2:-43046297 None:intergenic
AAACCGGGATGGTAATGGAC+CGG 0.576710 8_2:+43045291 Msa1248820:CDS
GAAGGGAAAAGATTAGTGAA+AGG 0.577890 8_2:+43046679 Msa1248820:CDS
GGTGGTGATGGTAAAGTTGA+AGG 0.578873 8_2:+43046199 Msa1248820:CDS
TCTCCTTGGACAGTGGAGAA+TGG 0.582146 8_2:-43044905 None:intergenic
TTTCCAGCGGCGATTAATGG+CGG 0.585609 8_2:+43045193 Msa1248820:CDS
GTTAATTCAAGACTCAATAC+CGG 0.586424 8_2:+43047175 Msa1248820:CDS
ATTGGCACCGCCATTACTGA+TGG 0.591223 8_2:-43045398 None:intergenic
TTAGGAGGGTCAGAAGGAGG+TGG 0.592041 8_2:-43046262 None:intergenic
AAGTGTGTTTCCATCAGTAA+TGG 0.593846 8_2:+43045388 Msa1248820:CDS
ATCACTTCAGCGCCAAGTGG+AGG 0.597164 8_2:+43047021 Msa1248820:CDS
TCTTGCTTAGGAGGGTCAGA+AGG 0.603501 8_2:-43046268 None:intergenic
GAGGTGGATTGGGACTAAGA+AGG 0.605645 8_2:+43045218 Msa1248820:CDS
TTCAAAACAACTCTTGACTC+CGG 0.616543 8_2:-43045310 None:intergenic
AACATTACCTTATTACAACC+AGG 0.621430 8_2:-43046726 None:intergenic
GCATCAGGGAACAAGAATGA+GGG 0.629320 8_2:+43046178 Msa1248820:CDS
GGATGTAATCTTGCTTAGGA+GGG 0.631066 8_2:-43046276 None:intergenic
TCCAGGAGCGATGATGAACG+AGG 0.631852 8_2:+43045111 Msa1248820:CDS
TAATGGCGGTGCCAATGCCG+TGG 0.636011 8_2:+43045405 Msa1248820:CDS
TGCTTAGGAGGGTCAGAAGG+AGG 0.637170 8_2:-43046265 None:intergenic
ATCCATGTTCGAGCTAGAAG+GGG 0.638122 8_2:+43046295 Msa1248820:CDS
GGACCCGGTTTAAACCGGGA+TGG 0.638557 8_2:+43045280 Msa1248820:CDS
GCTACAAGAGAGCCAAGCAG+AGG 0.640109 8_2:+43047825 Msa1248820:CDS
TGGTTACATATGCAGGTAGG+TGG 0.652770 8_2:+43047861 Msa1248820:CDS
GGAGATCTGGCAGTTTCCAG+CGG 0.658016 8_2:+43045180 Msa1248820:CDS
TCAATCACTTCAGCGCCAAG+TGG 0.659625 8_2:+43047018 Msa1248820:CDS
ATAGAATAGATACCTCCACT+TGG 0.671733 8_2:-43047033 None:intergenic
TACCACCACCAACACCACCA+TGG 0.672358 8_2:-43045333 None:intergenic
TGTGTTTCCATCAGTAATGG+CGG 0.675613 8_2:+43045391 Msa1248820:CDS
TCAGGGAACAAGAATGAGGG+TGG 0.675624 8_2:+43046181 Msa1248820:CDS
AGTCAGTTCAGGAAAGCATG+CGG 0.677155 8_2:+43046225 Msa1248820:CDS
GAATGGTTACATATGCAGGT+AGG 0.689550 8_2:+43047858 Msa1248820:CDS
CCAGGAGCGATGATGAACGA+GGG 0.693487 8_2:+43045112 Msa1248820:CDS
GAAAACGGACTAAAGCATCA+GGG 0.713041 8_2:+43046164 Msa1248820:CDS
CCTAAACCGTTCCCAAACTG+CGG 0.713413 8_2:-43045241 None:intergenic
ATTGTGCAGAATGATAGTGA+TGG 0.726537 8_2:+43046142 Msa1248820:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAAATATATGTATTTTG+GGG + chr8_2:43045523-43045542 Msa1248820:intron 10.0%
!! TTTAATGAATATACTTAAAA+CGG + chr8_2:43045584-43045603 Msa1248820:intron 10.0%
!!! TGTGTTTTTTATTTTATTAT+TGG + chr8_2:43045670-43045689 Msa1248820:intron 10.0%
!! TAACTAAATTATTACAATAA+AGG - chr8_2:43046057-43046076 None:intergenic 10.0%
!! TTAATGGAATTATTATTATT+TGG + chr8_2:43046119-43046138 Msa1248820:intron 10.0%
!!! AAAACATAATAAATTTTGAA+AGG - chr8_2:43047089-43047108 None:intergenic 10.0%
!!! TCATTTTTGTTTTATAATAT+AGG - chr8_2:43047982-43048001 None:intergenic 10.0%
!!! TAGTTTTTTTGTGTTATATT+TGG + chr8_2:43045452-43045471 Msa1248820:intron 15.0%
!! TTATGGAAAATGTAAATTTA+TGG + chr8_2:43046590-43046609 Msa1248820:intron 15.0%
!! ATAATAATAAACCAAATGAA+AGG + chr8_2:43046816-43046835 Msa1248820:intron 15.0%
!! AGTTTCAATATAAAAATCAA+GGG - chr8_2:43047241-43047260 None:intergenic 15.0%
!! AAACAAAAATGATATACTTA+AGG + chr8_2:43047990-43048009 Msa1248820:three_prime_UTR 15.0%
!!! TCTCTAGTTTTTTTTTCTTT+CGG + chr8_2:43044962-43044981 Msa1248820:five_prime_UTR 20.0%
!! AAAGAAAATAATGAATACCA+CGG - chr8_2:43045425-43045444 None:intergenic 20.0%
!!! TTTTTTGTGTTATATTTGGA+AGG + chr8_2:43045456-43045475 Msa1248820:intron 20.0%
!!! AAGATTTTGCTTCATTTTTT+TGG + chr8_2:43045717-43045736 Msa1248820:intron 20.0%
!!! AGATTTTGCTTCATTTTTTT+GGG + chr8_2:43045718-43045737 Msa1248820:intron 20.0%
!!! TTCACTTTTGATTTTAAAAC+TGG + chr8_2:43045835-43045854 Msa1248820:intron 20.0%
!!! AATTTTTCTCCAGAATAATT+AGG - chr8_2:43046034-43046053 None:intergenic 20.0%
!! ATATGTGTATTTGATGTATT+TGG + chr8_2:43046080-43046099 Msa1248820:intron 20.0%
!! TAAAAAGAGTTGTAAGTAAA+GGG - chr8_2:43046363-43046382 None:intergenic 20.0%
!! TTAAAAAGAGTTGTAAGTAA+AGG - chr8_2:43046364-43046383 None:intergenic 20.0%
!!! ATGATGTTAAATCTTGTTTA+TGG + chr8_2:43046573-43046592 Msa1248820:intron 20.0%
!! TTTAGTAATTTCCTTTCATT+TGG - chr8_2:43046830-43046849 None:intergenic 20.0%
!!! AACTGTTATTTTCTTTTCAT+GGG - chr8_2:43046877-43046896 None:intergenic 20.0%
!!! TAACTGTTATTTTCTTTTCA+TGG - chr8_2:43046878-43046897 None:intergenic 20.0%
!!! TTTCTTCAATTTAGGTTATT+GGG + chr8_2:43046960-43046979 Msa1248820:intron 20.0%
!!! ATCTATTCTATTTCTGATTT+TGG + chr8_2:43047045-43047064 Msa1248820:intron 20.0%
!! GAGTTTCAATATAAAAATCA+AGG - chr8_2:43047242-43047261 None:intergenic 20.0%
!!! GGAAAATTTTCAAATAAAGA+GGG - chr8_2:43047290-43047309 None:intergenic 20.0%
!!! AGGAAAATTTTCAAATAAAG+AGG - chr8_2:43047291-43047310 None:intergenic 20.0%
!!! TCCTTTTTTTTTTTTTTGGT+TGG + chr8_2:43048164-43048183 Msa1248820:three_prime_UTR 20.0%
!! ACCAACCAAAAAAAAAAAAA+AGG - chr8_2:43048168-43048187 None:intergenic 20.0%
!!! GATTAATGTTGAGTTGAAAA+CGG + chr8_2:43045052-43045071 Msa1248820:five_prime_UTR 25.0%
!!! CATTTTTTTGGGTACTTAAA+AGG + chr8_2:43045729-43045748 Msa1248820:intron 25.0%
!! TATGCAAACTTATTCAAGTT+TGG + chr8_2:43045858-43045877 Msa1248820:intron 25.0%
!!! TCAAAATTTTGTTTCGAACT+TGG + chr8_2:43045992-43046011 Msa1248820:intron 25.0%
!! TTGATGTATTTGGAATTAGT+CGG + chr8_2:43046090-43046109 Msa1248820:intron 25.0%
!!! ACTCTTATGTTATCGTTTTT+AGG + chr8_2:43046385-43046404 Msa1248820:intron 25.0%
! GAAAGGAAATTACTAAAAGT+AGG + chr8_2:43046833-43046852 Msa1248820:intron 25.0%
! ATTATAACTGACCAACAATT+AGG - chr8_2:43046908-43046927 None:intergenic 25.0%
!! CTTTCTTCAATTTAGGTTAT+TGG + chr8_2:43046959-43046978 Msa1248820:intron 25.0%
!! TGTTTAACACTTACATCTTT+TGG - chr8_2:43047217-43047236 None:intergenic 25.0%
! TGAACATTGGATTAATCTAA+GGG - chr8_2:43047602-43047621 None:intergenic 25.0%
! TTGAACATTGGATTAATCTA+AGG - chr8_2:43047603-43047622 None:intergenic 25.0%
! GATAAATATACTTGATTGAG+AGG + chr8_2:43047638-43047657 Msa1248820:intron 25.0%
! CATCATTGTTTCAAAAACAT+TGG + chr8_2:43047721-43047740 Msa1248820:intron 25.0%
!!! TTTTTTTTCTCTTTCCAGTA+TGG + chr8_2:43047759-43047778 Msa1248820:intron 25.0%
!! CATTTTATTATTACACGATG+AGG - chr8_2:43047921-43047940 None:intergenic 25.0%
!!! TATAATATAGGAGCATTTGA+AGG - chr8_2:43047970-43047989 None:intergenic 25.0%
!!! AGGCTCCTTTTTTTTTTTTT+TGG + chr8_2:43048160-43048179 Msa1248820:three_prime_UTR 25.0%
!! TATTTTGGGGAGTGATTAAT+TGG + chr8_2:43045536-43045555 Msa1248820:intron 30.0%
! CAGTGTTCAACTTGTATTTT+TGG + chr8_2:43045626-43045645 Msa1248820:intron 30.0%
AAATGAAGCAAAATCTTGAG+CGG - chr8_2:43045714-43045733 None:intergenic 30.0%
! TTCACAAACTATTTTGCCTA+TGG + chr8_2:43045786-43045805 Msa1248820:intron 30.0%
TCACAAAACGGTAGATAATT+AGG - chr8_2:43045959-43045978 None:intergenic 30.0%
TTGCAATCATTGTCACAAAA+CGG - chr8_2:43045971-43045990 None:intergenic 30.0%
AATGGTTGTCCTAATTATTC+TGG + chr8_2:43046022-43046041 Msa1248820:intron 30.0%
AATTAGTCGGTGTGATTTAA+TGG + chr8_2:43046103-43046122 Msa1248820:intron 30.0%
! AGAATGATAGTGATGGAAAA+CGG + chr8_2:43046149-43046168 Msa1248820:intron 30.0%
ACTTCTCAATATCAAGAGTT+AGG + chr8_2:43046536-43046555 Msa1248820:intron 30.0%
TGAAAGGATGAAAATTCTAC+AGG + chr8_2:43046695-43046714 Msa1248820:CDS 30.0%
! TTTAGTTCCTGGTTGTAATA+AGG + chr8_2:43046719-43046738 Msa1248820:CDS 30.0%
AACATTACCTTATTACAACC+AGG - chr8_2:43046729-43046748 None:intergenic 30.0%
TGAGAGCAGAAAAAATTAAC+AGG - chr8_2:43046759-43046778 None:intergenic 30.0%
TGCTCTCATATTCACATAAA+CGG + chr8_2:43046771-43046790 Msa1248820:intron 30.0%
GTTATGATCTTCCTAATTGT+TGG + chr8_2:43046894-43046913 Msa1248820:intron 30.0%
GTTGAGTCTTTCTTCAATTT+AGG + chr8_2:43046952-43046971 Msa1248820:intron 30.0%
GTTAATTCAAGACTCAATAC+CGG + chr8_2:43047175-43047194 Msa1248820:CDS 30.0%
! TTAACACTTACATCTTTTGG+AGG - chr8_2:43047214-43047233 None:intergenic 30.0%
TGTTGCAAGTATGTATAACA+AGG + chr8_2:43047366-43047385 Msa1248820:intron 30.0%
TACAGCTAAAGATTGAACAT+TGG - chr8_2:43047615-43047634 None:intergenic 30.0%
GAGGACACAGATTAAAAATT+TGG + chr8_2:43047657-43047676 Msa1248820:intron 30.0%
CTGGAAAGAGAAAAAAAACT+AGG - chr8_2:43047757-43047776 None:intergenic 30.0%
CATACCTTTGTGTAGAAAAA+GGG - chr8_2:43048046-43048065 None:intergenic 30.0%
CTCAATATTGTGTACTATAG+TGG + chr8_2:43048131-43048150 Msa1248820:three_prime_UTR 30.0%
!! ATGTTGAGTTGAAAACGGTT+CGG + chr8_2:43045057-43045076 Msa1248820:five_prime_UTR 35.0%
TTGAAGTTAGTGAACCGAAA+TGG + chr8_2:43045087-43045106 Msa1248820:exon 35.0%
TTCAAAACAACTCTTGACTC+CGG - chr8_2:43045313-43045332 None:intergenic 35.0%
!!! TCAAGAGTTGTTTTGAACCA+TGG + chr8_2:43045316-43045335 Msa1248820:CDS 35.0%
AAGTGTGTTTCCATCAGTAA+TGG + chr8_2:43045388-43045407 Msa1248820:CDS 35.0%
AATAATGAATACCACGGCAT+TGG - chr8_2:43045419-43045438 None:intergenic 35.0%
AATGAAGCAAAATCTTGAGC+GGG - chr8_2:43045713-43045732 None:intergenic 35.0%
! GTTTGAGATTACACTTCCAT+AGG - chr8_2:43045805-43045824 None:intergenic 35.0%
! CAGCATAAGGACCAAAATTT+TGG + chr8_2:43045913-43045932 Msa1248820:intron 35.0%
TTCGAACTTGGATGTCTAAA+TGG + chr8_2:43046004-43046023 Msa1248820:intron 35.0%
! ATTGTGCAGAATGATAGTGA+TGG + chr8_2:43046142-43046161 Msa1248820:intron 35.0%
ACATGGATGTAATCTTGCTT+AGG - chr8_2:43046283-43046302 None:intergenic 35.0%
ACTCTTGATATTGAGAAGTG+CGG - chr8_2:43046535-43046554 None:intergenic 35.0%
!! TCTATGCCTCTGTTTCTAAT+TGG + chr8_2:43046630-43046649 Msa1248820:intron 35.0%
AACATGCCAATTAGAAACAG+AGG - chr8_2:43046639-43046658 None:intergenic 35.0%
!! TCTGTTTCTAATTGGCATGT+TGG + chr8_2:43046638-43046657 Msa1248820:intron 35.0%
GAAGGGAAAAGATTAGTGAA+AGG + chr8_2:43046679-43046698 Msa1248820:CDS 35.0%
! ATTCTACAGGATTTAGTTCC+TGG + chr8_2:43046708-43046727 Msa1248820:CDS 35.0%
! TTAGGTTATTGGGAAAGCAT+TGG + chr8_2:43046970-43046989 Msa1248820:intron 35.0%
ATAGAATAGATACCTCCACT+TGG - chr8_2:43047036-43047055 None:intergenic 35.0%
!! ACTTACATCTTTTGGAGGAA+AGG - chr8_2:43047209-43047228 None:intergenic 35.0%
! ATTTTCCTTCTGACAAGACA+TGG + chr8_2:43047303-43047322 Msa1248820:intron 35.0%
ACTCATGAGTATAGTAGTTC+TGG + chr8_2:43047334-43047353 Msa1248820:intron 35.0%
TGAAGAATGTCTGCAAAAGA+TGG - chr8_2:43047397-43047416 None:intergenic 35.0%
ATGAATGATAGCTTCTCACT+AGG + chr8_2:43047498-43047517 Msa1248820:intron 35.0%
TGTTCAATCTTTAGCTGTAG+AGG + chr8_2:43047616-43047635 Msa1248820:intron 35.0%
! CTTGTAGCTTGTGAAACAAA+TGG - chr8_2:43047813-43047832 None:intergenic 35.0%
! CTTAAGGTGTTAAAGTCCAA+TGG + chr8_2:43048006-43048025 Msa1248820:three_prime_UTR 35.0%
! CATTCCCTTTTTCTACACAA+AGG + chr8_2:43048039-43048058 Msa1248820:three_prime_UTR 35.0%
CCATACCTTTGTGTAGAAAA+AGG - chr8_2:43048047-43048066 None:intergenic 35.0%
! CCTTTTTCTACACAAAGGTA+TGG + chr8_2:43048044-43048063 Msa1248820:three_prime_UTR 35.0%
! GAGTTGTTACTGTTTTCAAC+AGG + chr8_2:43048066-43048085 Msa1248820:three_prime_UTR 35.0%
!! GAGAGAAAGAGTGTTAGGTT+CGG - chr8_2:43044938-43044957 None:intergenic 40.0%
!! AGAGAGAGAGAAAGAGTGTT+AGG - chr8_2:43044943-43044962 None:intergenic 40.0%
GTCGTATTTCCATTCCCATT+CGG - chr8_2:43045145-43045164 None:intergenic 40.0%
CAGATCTCCGCTAAACTAAA+CGG - chr8_2:43045169-43045188 None:intergenic 40.0%
! TTAGGACAGTTTGGTGAGTT+TGG + chr8_2:43045259-43045278 Msa1248820:CDS 40.0%
ATTACCATCCCGGTTTAAAC+CGG - chr8_2:43045287-43045306 None:intergenic 40.0%
!!! AGAGTTGTTTTGAACCATGG+TGG + chr8_2:43045319-43045338 Msa1248820:CDS 40.0%
TGTGTTTCCATCAGTAATGG+CGG + chr8_2:43045391-43045410 Msa1248820:CDS 40.0%
CAAGTTTGGTCTCGATTTCA+AGG + chr8_2:43045872-43045891 Msa1248820:intron 40.0%
GATGTCTTTGCTACAGCATA+AGG + chr8_2:43045900-43045919 Msa1248820:intron 40.0%
! GAGCGAAGTAGCCAAAATTT+TGG - chr8_2:43045927-43045946 None:intergenic 40.0%
!! GAAAACGGACTAAAGCATCA+GGG + chr8_2:43046164-43046183 Msa1248820:CDS 40.0%
GGATGTAATCTTGCTTAGGA+GGG - chr8_2:43046279-43046298 None:intergenic 40.0%
TGGATGTAATCTTGCTTAGG+AGG - chr8_2:43046280-43046299 None:intergenic 40.0%
CTATGATGTCACAGCCAAAA+CGG - chr8_2:43046480-43046499 None:intergenic 40.0%
GTTTCTAATTGGCATGTTGG+TGG + chr8_2:43046641-43046660 Msa1248820:intron 40.0%
! CATGTTGGTGGACAATAAAC+AGG + chr8_2:43046653-43046672 Msa1248820:intron 40.0%
TGGACAATAAACAGGCTAGA+AGG + chr8_2:43046661-43046680 Msa1248820:intron 40.0%
GGACAATAAACAGGCTAGAA+GGG + chr8_2:43046662-43046681 Msa1248820:intron 40.0%
! AGTAGGAAAAACGTGCTACT+TGG + chr8_2:43046850-43046869 Msa1248820:intron 40.0%
!! GTAGGAAAAACGTGCTACTT+GGG + chr8_2:43046851-43046870 Msa1248820:intron 40.0%
TTTAAGAAGCTTGCAACCTC+TGG + chr8_2:43047541-43047560 Msa1248820:intron 40.0%
! CAAACGTTTGTTGACCATAC+TGG - chr8_2:43047776-43047795 None:intergenic 40.0%
CAACAAACGTTTGATCTTGC+CGG + chr8_2:43047783-43047802 Msa1248820:CDS 40.0%
TTGTGAAACAAATGGCATGC+CGG - chr8_2:43047805-43047824 None:intergenic 40.0%
ACCAGAATGGTTACATATGC+AGG + chr8_2:43047854-43047873 Msa1248820:CDS 40.0%
ACCTGCATATGTAACCATTC+TGG - chr8_2:43047858-43047877 None:intergenic 40.0%
GAATGGTTACATATGCAGGT+AGG + chr8_2:43047858-43047877 Msa1248820:CDS 40.0%
! GGAGCATTTGAAGGATGAAA+TGG - chr8_2:43047961-43047980 None:intergenic 40.0%
!! GGAATGAAGAAAGTGTCCAT+TGG - chr8_2:43048025-43048044 None:intergenic 40.0%
!! GTGTACTATAGTGGACTTTC+AGG + chr8_2:43048140-43048159 Msa1248820:three_prime_UTR 40.0%
TCGTGTTTGTTTGTGTAGCA+AGG + chr8_2:43048280-43048299 Msa1248820:three_prime_UTR 40.0%
!! GAGTTGAAAACGGTTCGGTT+CGG + chr8_2:43045062-43045081 Msa1248820:five_prime_UTR 45.0%
!! AGTTGAAAACGGTTCGGTTC+GGG + chr8_2:43045063-43045082 Msa1248820:five_prime_UTR 45.0%
TAGTGAACCGAAATGGATCC+AGG + chr8_2:43045094-43045113 Msa1248820:exon 45.0%
AACGAGGGTTCATTTCCGAA+TGG + chr8_2:43045127-43045146 Msa1248820:CDS 45.0%
ACGAGGGTTCATTTCCGAAT+GGG + chr8_2:43045128-43045147 Msa1248820:CDS 45.0%
GGTTCATTTCCGAATGGGAA+TGG + chr8_2:43045133-43045152 Msa1248820:CDS 45.0%
! TACGACGCCGTTTAGTTTAG+CGG + chr8_2:43045159-43045178 Msa1248820:CDS 45.0%
! CGTTTAGTTTAGCGGAGATC+TGG + chr8_2:43045167-43045186 Msa1248820:CDS 45.0%
AGTTTGGACCCGGTTTAAAC+CGG + chr8_2:43045275-43045294 Msa1248820:CDS 45.0%
TTACCATCCCGGTTTAAACC+GGG - chr8_2:43045286-43045305 None:intergenic 45.0%
GGTTTAAACCGGGATGGTAA+TGG + chr8_2:43045286-43045305 Msa1248820:CDS 45.0%
!! GTTTTGAACCATGGTGGTGT+TGG + chr8_2:43045325-43045344 Msa1248820:CDS 45.0%
!! GGAAAACGGACTAAAGCATC+AGG + chr8_2:43046163-43046182 Msa1248820:CDS 45.0%
AGCATCAGGGAACAAGAATG+AGG + chr8_2:43046177-43046196 Msa1248820:CDS 45.0%
GCATCAGGGAACAAGAATGA+GGG + chr8_2:43046178-43046197 Msa1248820:CDS 45.0%
AACAAGAATGAGGGTGGTGA+TGG + chr8_2:43046187-43046206 Msa1248820:CDS 45.0%
!! GGTGGTGATGGTAAAGTTGA+AGG + chr8_2:43046199-43046218 Msa1248820:CDS 45.0%
GTTGAAGGAGAAGTCAGTTC+AGG + chr8_2:43046214-43046233 Msa1248820:CDS 45.0%
! AGTCAGTTCAGGAAAGCATG+CGG + chr8_2:43046225-43046244 Msa1248820:CDS 45.0%
ACATCCATGTTCGAGCTAGA+AGG + chr8_2:43046293-43046312 Msa1248820:CDS 45.0%
CATCCATGTTCGAGCTAGAA+GGG + chr8_2:43046294-43046313 Msa1248820:CDS 45.0%
ATCCATGTTCGAGCTAGAAG+GGG + chr8_2:43046295-43046314 Msa1248820:CDS 45.0%
TTACGCGTTCTGCAAGACTA+TGG - chr8_2:43046336-43046355 None:intergenic 45.0%
!!! CGTTTTGGCTGTGACATCAT+AGG + chr8_2:43046478-43046497 Msa1248820:intron 45.0%
AACCTCTGGTCTCTGCATAA+TGG + chr8_2:43047555-43047574 Msa1248820:intron 45.0%
ACCTCTGGTCTCTGCATAAT+GGG + chr8_2:43047556-43047575 Msa1248820:intron 45.0%
TGGTTACATATGCAGGTAGG+TGG + chr8_2:43047861-43047880 Msa1248820:CDS 45.0%
TTACATATGCAGGTAGGTGG+TGG + chr8_2:43047864-43047883 Msa1248820:CDS 45.0%
!!! AATATTAAATATATGTATTT+TGG + chr8_2:43045521-43045540 Msa1248820:intron 5.0%
!!! ATATTAAATATATGTATTTT+GGG + chr8_2:43045522-43045541 Msa1248820:intron 5.0%
TCTCCTTGGACAGTGGAGAA+TGG - chr8_2:43044908-43044927 None:intergenic 50.0%
CATCGCTCCTGGATCCATTT+CGG - chr8_2:43045104-43045123 None:intergenic 50.0%
CAGTTTCCAGCGGCGATTAA+TGG + chr8_2:43045190-43045209 Msa1248820:CDS 50.0%
TTTCCAGCGGCGATTAATGG+CGG + chr8_2:43045193-43045212 Msa1248820:CDS 50.0%
!! GAGGTGGATTGGGACTAAGA+AGG + chr8_2:43045218-43045237 Msa1248820:CDS 50.0%
GACTAAGAAGGCCGCAGTTT+GGG + chr8_2:43045230-43045249 Msa1248820:CDS 50.0%
CCTAAACCGTTCCCAAACTG+CGG - chr8_2:43045244-43045263 None:intergenic 50.0%
! GGGAACGGTTTAGGACAGTT+TGG + chr8_2:43045250-43045269 Msa1248820:CDS 50.0%
! CAGTTTGGTGAGTTTGGACC+CGG + chr8_2:43045265-43045284 Msa1248820:CDS 50.0%
GTTTGGACCCGGTTTAAACC+GGG + chr8_2:43045276-43045295 Msa1248820:CDS 50.0%
!! AAACCGGGATGGTAATGGAC+CGG + chr8_2:43045291-43045310 Msa1248820:CDS 50.0%
! TTGAACCATGGTGGTGTTGG+TGG + chr8_2:43045328-43045347 Msa1248820:CDS 50.0%
ATTGGCACCGCCATTACTGA+TGG - chr8_2:43045401-43045420 None:intergenic 50.0%
TCAGGGAACAAGAATGAGGG+TGG + chr8_2:43046181-43046200 Msa1248820:CDS 50.0%
TCTTGCTTAGGAGGGTCAGA+AGG - chr8_2:43046271-43046290 None:intergenic 50.0%
!!! TGAGTTGAGAAGCGCCGTTT+TGG + chr8_2:43046463-43046482 Msa1248820:intron 50.0%
TCAATCACTTCAGCGCCAAG+TGG + chr8_2:43047018-43047037 Msa1248820:CDS 50.0%
TGGAGGAAAGGCTTCAATGC+CGG - chr8_2:43047197-43047216 None:intergenic 50.0%
GTGAGCCATGTCTTGTCAGA+AGG - chr8_2:43047311-43047330 None:intergenic 50.0%
TCTGGTGAAGAACCTCTGCT+TGG - chr8_2:43047840-43047859 None:intergenic 50.0%
GCAGAGGTTCTTCACCAGAA+TGG + chr8_2:43047841-43047860 Msa1248820:CDS 50.0%
CAACAGGCTGCTTCAGTTTG+TGG + chr8_2:43048082-43048101 Msa1248820:three_prime_UTR 50.0%
GCACCATTCTCCACTGTCCA+AGG + chr8_2:43044902-43044921 Msa1248820:five_prime_UTR 55.0%
TGCGTGATCTCCTTGGACAG+TGG - chr8_2:43044915-43044934 None:intergenic 55.0%
TCCAGGAGCGATGATGAACG+AGG + chr8_2:43045111-43045130 Msa1248820:CDS 55.0%
CCAGGAGCGATGATGAACGA+GGG + chr8_2:43045112-43045131 Msa1248820:CDS 55.0%
GGAGATCTGGCAGTTTCCAG+CGG + chr8_2:43045180-43045199 Msa1248820:CDS 55.0%
GGACTAAGAAGGCCGCAGTT+TGG + chr8_2:43045229-43045248 Msa1248820:CDS 55.0%
AGAAGGCCGCAGTTTGGGAA+CGG + chr8_2:43045235-43045254 Msa1248820:CDS 55.0%
CCGCAGTTTGGGAACGGTTT+AGG + chr8_2:43045241-43045260 Msa1248820:CDS 55.0%
ACTCCGGTCCATTACCATCC+CGG - chr8_2:43045297-43045316 None:intergenic 55.0%
!! AACCATGGTGGTGTTGGTGG+TGG + chr8_2:43045331-43045350 Msa1248820:CDS 55.0%
TACCACCACCAACACCACCA+TGG - chr8_2:43045336-43045355 None:intergenic 55.0%
TTAGGAGGGTCAGAAGGAGG+TGG - chr8_2:43046265-43046284 None:intergenic 55.0%
TGCTTAGGAGGGTCAGAAGG+AGG - chr8_2:43046268-43046287 None:intergenic 55.0%
!! GACCCCTTCTAGCTCGAACA+TGG - chr8_2:43046300-43046319 None:intergenic 55.0%
ATCACTTCAGCGCCAAGTGG+AGG + chr8_2:43047021-43047040 Msa1248820:CDS 55.0%
GCCCATTATGCAGAGACCAG+AGG - chr8_2:43047560-43047579 None:intergenic 55.0%
GCTACAAGAGAGCCAAGCAG+AGG + chr8_2:43047825-43047844 Msa1248820:CDS 55.0%
!! GGTTCGGTGCGTGATCTCCT+TGG - chr8_2:43044922-43044941 None:intergenic 60.0%
CCCTCGTTCATCATCGCTCC+TGG - chr8_2:43045115-43045134 None:intergenic 60.0%
TAATGGCGGCGGAGGTGGAT+TGG + chr8_2:43045207-43045226 Msa1248820:CDS 60.0%
! AATGGCGGCGGAGGTGGATT+GGG + chr8_2:43045208-43045227 Msa1248820:CDS 60.0%
GGACCCGGTTTAAACCGGGA+TGG + chr8_2:43045280-43045299 Msa1248820:CDS 60.0%
! TAATGGCGGTGCCAATGCCG+TGG + chr8_2:43045405-43045424 Msa1248820:CDS 60.0%
CCAGCGGCGATTAATGGCGG+CGG + chr8_2:43045196-43045215 Msa1248820:CDS 65.0%
GCGATTAATGGCGGCGGAGG+TGG + chr8_2:43045202-43045221 Msa1248820:CDS 65.0%
CCGCCGCCATTAATCGCCGC+TGG - chr8_2:43045199-43045218 None:intergenic 70.0%
GCGGCGATTAATGGCGGCGG+AGG + chr8_2:43045199-43045218 Msa1248820:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 43044850 43048335 43044850 ID=Msa1248820;Name=Msa1248820
chr8_2 mRNA 43044850 43048335 43044850 ID=Msa1248820-mRNA-1;Parent=Msa1248820;Name=Msa1248820-mRNA-1;_AED=0.05;_eAED=0.05;_QI=256|1|1|1|1|1|6|430|285
chr8_2 exon 43044850 43045426 43044850 ID=Msa1248820-mRNA-1:exon:13110;Parent=Msa1248820-mRNA-1
chr8_2 exon 43046151 43046351 43046151 ID=Msa1248820-mRNA-1:exon:13111;Parent=Msa1248820-mRNA-1
chr8_2 exon 43046675 43046740 43046675 ID=Msa1248820-mRNA-1:exon:13112;Parent=Msa1248820-mRNA-1
chr8_2 exon 43046974 43047042 43046974 ID=Msa1248820-mRNA-1:exon:13113;Parent=Msa1248820-mRNA-1
chr8_2 exon 43047151 43047222 43047151 ID=Msa1248820-mRNA-1:exon:13114;Parent=Msa1248820-mRNA-1
chr8_2 exon 43047777 43048335 43047777 ID=Msa1248820-mRNA-1:exon:13115;Parent=Msa1248820-mRNA-1
chr8_2 five_prime_UTR 43044850 43045105 43044850 ID=Msa1248820-mRNA-1:five_prime_utr;Parent=Msa1248820-mRNA-1
chr8_2 CDS 43045106 43045426 43045106 ID=Msa1248820-mRNA-1:cds;Parent=Msa1248820-mRNA-1
chr8_2 CDS 43046151 43046351 43046151 ID=Msa1248820-mRNA-1:cds;Parent=Msa1248820-mRNA-1
chr8_2 CDS 43046675 43046740 43046675 ID=Msa1248820-mRNA-1:cds;Parent=Msa1248820-mRNA-1
chr8_2 CDS 43046974 43047042 43046974 ID=Msa1248820-mRNA-1:cds;Parent=Msa1248820-mRNA-1
chr8_2 CDS 43047151 43047222 43047151 ID=Msa1248820-mRNA-1:cds;Parent=Msa1248820-mRNA-1
chr8_2 CDS 43047777 43047905 43047777 ID=Msa1248820-mRNA-1:cds;Parent=Msa1248820-mRNA-1
chr8_2 three_prime_UTR 43047906 43048335 43047906 ID=Msa1248820-mRNA-1:three_prime_utr;Parent=Msa1248820-mRNA-1
Gene Sequence

>Msa1248820

ATGGATCCAGGAGCGATGATGAACGAGGGTTCATTTCCGAATGGGAATGGAAATACGACGCCGTTTAGTTTAGCGGAGATCTGGCAGTTTCCAGCGGCGATTAATGGCGGCGGAGGTGGATTGGGACTAAGAAGGCCGCAGTTTGGGAACGGTTTAGGACAGTTTGGTGAGTTTGGACCCGGTTTAAACCGGGATGGTAATGGACCGGAGTCAAGAGTTGTTTTGAACCATGGTGGTGTTGGTGGTGGTAAGAAGAGACGTGACTCTGAAGATGATTCTGCTAAGTGTGTTTCCATCAGTAATGGCGGTGCCAATGCCGTGAATGATAGTGATGGAAAACGGACTAAAGCATCAGGGAACAAGAATGAGGGTGGTGATGGTAAAGTTGAAGGAGAAGTCAGTTCAGGAAAGCATGCGGAGCAAAGCAGTAAGCCACCTCCTTCTGACCCTCCTAAGCAAGATTACATCCATGTTCGAGCTAGAAGGGGTCAAGCTACTGATAGCCATAGTCTTGCAGAACGCGCTAGAAGGGAAAAGATTAGTGAAAGGATGAAAATTCTACAGGATTTAGTTCCTGGTTGTAATAAGGTTATTGGGAAAGCATTGGTGCTTGATGAGATAATTAATTATATTCAATCACTTCAGCGCCAAGTGGAGTTTTTATCGATGAAACTTGAAGCAGTTAATTCAAGACTCAATACCGGCATTGAAGCCTTTCCTCCAAAAGATTATGGTCAACAAACGTTTGATCTTGCCGGCATGCCATTTGTTTCACAAGCTACAAGAGAGCCAAGCAGAGGTTCTTCACCAGAATGGTTACATATGCAGGTAGGTGGTGGTTTCGAAAGAACGACGTAG
Protein sequence

>Msa1248820

MDPGAMMNEGSFPNGNGNTTPFSLAEIWQFPAAINGGGGGLGLRRPQFGNGLGQFGEFGPGLNRDGNGPESRVVLNHGGVGGGKKRRDSEDDSAKCVSISNGGANAVNDSDGKRTKASGNKNEGGDGKVEGEVSSGKHAEQSSKPPPSDPPKQDYIHVRARRGQATDSHSLAERARREKISERMKILQDLVPGCNKVIGKALVLDEIINYIQSLQRQVEFLSMKLEAVNSRLNTGIEAFPPKDYGQQTFDLAGMPFVSQATREPSRGSSPEWLHMQVGGGFERTT*