AlfalfaGEDB | Msa1249960

M. sativa cultivar zhongmu-4 / Msa1249960

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0032502 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1249960	XP_039685363.1	90.088	565	29	4	124	679	126	672	0.0	897

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1249960	sp\|Q9CAL8\|PEK13_ARATH	60.950	484	165	8	212	675	230	709	0.0	570

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1249960	MtrunA17_Chr5g0413511	71.355	391	108	3	263	653	7	393	0.0	579
Msa1249960	MtrunA17_Chr7g0221191	48.936	611	226	12	64	647	172	723	1.27e-177	524
Msa1249960	MtrunA17_Chr8g0365691	87.000	400	24	5	94	483	96	477	2.86e-167	488
Msa1249960	MtrunA17_Chr6g0485761	58.886	377	139	2	301	669	278	646	1.34e-149	449
Msa1249960	MtrunA17_Chr0c01g0489481	54.128	436	181	7	203	631	219	642	5.00e-147	442
Msa1249960	MtrunA17_Chr8g0373501	60.542	332	119	3	305	625	324	654	3.84e-143	432
Msa1249960	MtrunA17_Chr3g0144481	58.757	354	128	5	292	627	55	408	3.18e-141	420
Msa1249960	MtrunA17_Chr8g0365701	95.408	196	9	0	484	679	20	215	5.06e-135	395
Msa1249960	MtrunA17_Chr6g0485721	58.632	307	125	1	306	612	86	390	2.54e-121	368
Msa1249960	MtrunA17_Chr5g0443121	51.557	289	134	2	306	592	171	455	2.13e-97	309

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1249960	AT1G70460.1	60.950	484	165	8	212	675	230	709	0.0	570
Msa1249960	AT1G23540.1	60.041	483	158	8	218	675	247	719	0.0	565
Msa1249960	AT1G10620.1	58.678	484	172	9	205	676	250	717	0.0	545
Msa1249960	AT5G38560.1	54.444	450	186	5	214	657	235	671	5.03e-166	492
Msa1249960	AT1G70450.1	65.812	351	118	2	294	642	25	375	3.65e-161	469
Msa1249960	AT1G68690.1	54.988	431	176	5	207	631	270	688	8.31e-160	477
Msa1249960	AT1G68690.3	54.988	431	176	5	207	631	270	688	8.31e-160	477
Msa1249960	AT1G68690.2	54.988	431	176	5	207	631	270	688	8.31e-160	477
Msa1249960	AT1G26150.1	54.279	444	181	6	218	651	330	761	1.74e-159	478
Msa1249960	AT3G24550.1	58.824	374	133	3	306	668	268	631	5.86e-149	447

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Find 189 sgRNAs with CRISPR-Local

Find 337 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCATGCATGCAAAATC+AGG	0.328251	8_2:-45170990	Msa1249960:CDS
AAAGCTATCTGAGCACTTAT+TGG	0.281181	8_2:+45169909	None:intergenic
AAAGGACCTCCTCCTCCTAA+GGG	0.572326	8_2:+45169945	None:intergenic
AAATCTCAACAGTAATAGCA+AGG	0.619797	8_2:-45171576	Msa1249960:CDS
AACAACGGAGGTGGCGGTGA+TGG	0.502435	8_2:+45171278	None:intergenic
AACGGAGGTGGCGGTGATGG+TGG	0.543301	8_2:+45171281	None:intergenic
AAGACTCTTAAGGCTGGTAG+CGG	0.602983	8_2:-45169768	Msa1249960:CDS
AAGAGGCTAAAGATAGCTAT+TGG	0.492722	8_2:-45169229	Msa1249960:CDS
AAGATGTAGAAGATTTCTCC+GGG	0.535793	8_2:+45171141	None:intergenic
AAGCCATGCATGCAAAATCA+GGG	0.610002	8_2:-45170989	Msa1249960:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCTCAACAGTAATAGCA+AGG	-	chr8_2:45167187-45167206	Msa1249960:CDS	30.0%
TGGCAAAAAAAAGGATGATA+AGG	-	chr8_2:45167286-45167305	Msa1249960:CDS	30.0%
TGCGAAATTCAAATGTAACA+GGG	+	chr8_2:45168015-45168034	None:intergenic	30.0%
TTTATATTGCAGTAAAACGC+AGG	-	chr8_2:45168077-45168096	Msa1249960:CDS	30.0%
AACTGTAATTACAACAGCTT+TGG	+	chr8_2:45168112-45168131	None:intergenic	30.0%
TTGAAAAAAAATGCTGACGA+GGG	+	chr8_2:45168310-45168329	None:intergenic	30.0%
ATTGAAAAAAAATGCTGACG+AGG	+	chr8_2:45168311-45168330	None:intergenic	30.0%
ATTATCAGACTATACTTCCA+TGG	-	chr8_2:45168353-45168372	Msa1249960:intron	30.0%
GTATAATCTGGTAGCAATTA+CGG	-	chr8_2:45168540-45168559	Msa1249960:intron	30.0%
TACTCATAATCTCATGCTTT+CGG	-	chr8_2:45168564-45168583	Msa1249960:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	45167179	45171606	45167179	ID=Msa1249960;Name=Msa1249960
chr8_2	mRNA	45167179	45171606	45167179	ID=Msa1249960-mRNA-1;Parent=Msa1249960;Name=Msa1249960-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0\|0\|1\|1\|1\|8\|0\|679
chr8_2	exon	45170832	45171606	45170832	ID=Msa1249960-mRNA-1:exon:13669;Parent=Msa1249960-mRNA-1
chr8_2	exon	45169613	45170050	45169613	ID=Msa1249960-mRNA-1:exon:13668;Parent=Msa1249960-mRNA-1
chr8_2	exon	45169194	45169280	45169194	ID=Msa1249960-mRNA-1:exon:13667;Parent=Msa1249960-mRNA-1
chr8_2	exon	45168968	45169038	45168968	ID=Msa1249960-mRNA-1:exon:13666;Parent=Msa1249960-mRNA-1
chr8_2	exon	45168775	45168851	45168775	ID=Msa1249960-mRNA-1:exon:13665;Parent=Msa1249960-mRNA-1
chr8_2	exon	45168065	45168212	45168065	ID=Msa1249960-mRNA-1:exon:13664;Parent=Msa1249960-mRNA-1
chr8_2	exon	45167707	45167868	45167707	ID=Msa1249960-mRNA-1:exon:13663;Parent=Msa1249960-mRNA-1
chr8_2	exon	45167179	45167460	45167179	ID=Msa1249960-mRNA-1:exon:13662;Parent=Msa1249960-mRNA-1
chr8_2	CDS	45170832	45171606	45170832	ID=Msa1249960-mRNA-1:cds;Parent=Msa1249960-mRNA-1
chr8_2	CDS	45169613	45170050	45169613	ID=Msa1249960-mRNA-1:cds;Parent=Msa1249960-mRNA-1
chr8_2	CDS	45169194	45169280	45169194	ID=Msa1249960-mRNA-1:cds;Parent=Msa1249960-mRNA-1
chr8_2	CDS	45168968	45169038	45168968	ID=Msa1249960-mRNA-1:cds;Parent=Msa1249960-mRNA-1
chr8_2	CDS	45168775	45168851	45168775	ID=Msa1249960-mRNA-1:cds;Parent=Msa1249960-mRNA-1
chr8_2	CDS	45168065	45168212	45168065	ID=Msa1249960-mRNA-1:cds;Parent=Msa1249960-mRNA-1
chr8_2	CDS	45167707	45167868	45167707	ID=Msa1249960-mRNA-1:cds;Parent=Msa1249960-mRNA-1
chr8_2	CDS	45167179	45167460	45167179	ID=Msa1249960-mRNA-1:cds;Parent=Msa1249960-mRNA-1

Gene Sequence

>Msa1249960

ATGGCTCCAAATCTCAACAGTAATAGCAAGGTTTCTTCTTCTTCATCATCTTCTCTTTTATCTTTTTCTTCTCCATTTTTACCTTTATTTGAAAATAAGAAAGATTTTGGCAAAAAAAAGGATGATAAGGATAAAGGCAAGGATAAAGCTCCATCACCATCTAAGCCTGAGCCTCCGGAAAGTTCTCCGTCACACTCGCCACCACCACCAAAGTCTCGTTCACCGCCTAAAGTTTTACCTCCACCACCGTCACCACCACTACCGTCACCACCGCCACCCTCTCCACCACCAAACTCACCTCCACCACCATCACCGCCACCTCCGTTGTTATCTCCACCTCCAGCTTCACCTCCTCCGCCACCAATGCCCAATGCACAATATTCTGCTTCTGCTTTATCATCTCCTAATTCTCCCCGTAAACAACCACCTCCGTCATTGCCTCCCCCGGAGAAATCTTCTACATCTTCATCATCATCATCACCATCATCATCGCCATCACCATCACCATCATCATCATCATCTTCTTCTTCTTCTTCATCTTCTTCTTCTTCTTCTTCGTCTTCTTCTTCTACGTCCTCGAAGGATTCGCCACATAAAGCCATGCATGCAAAATCAGGGAGTTCTCATTCCTCTGGCCACACGCCCGGTGAAATAGCTGGTTATGCTGTTGCCGCAGTTTTGGTTGTCGCCATAGTTGCGGCAGCTGTTTTTTTTGCTTTTAGGAAGAAGAAAGCGAAAGGGGATGCATATGTTGGACCCTATATACCACCCCCTGGTGCTAATGGACATTACTATGTACAGCAACCTTCCCTTGGAAACATCTATGGCAATGTGAACGTTGAAGGACGCAGCACGAAGCCCTTAGGAGGAGGAGGTCCTTTAGATCCGGCGCAGCCAATAAGTGCTCAGATAGCTTTTACTTATGACATGGTAATGGAAATAACCAACGCATTTTCTAGTCAAAATGTGATAGGGCAAGGAGGATTTGGGTGTGTTTACAAGGGTTGGCTGAAGGATGGAAAAGAAGTGGCAGTCAAGACTCTTAAGGCTGGTAGCGGGCAGGGAGATAGGGAGTTCAGGGCCGAAGTGGAAATCATTAGCCGTGTTCATCATCGGCATTTGGTATCGTTGGTTGGTTATTGTATATCTGAGGAGCAAAGAGTGCTTATCTATGAGTTTGTTCCTAATGGAAATCTTCATCATCACTTGCATGGAAGTGGAATGCCAGTGTTGGCTTGGGACAAGAGGCTAAAGATAGCTATTGGTGCTGCAAAAGGCCTGGCATATCTGCATGAGGACTGCAGCCAGAAGATTATTCACAGAGATATTAAATCCGCCAACATACTATTGGATGATGCTTTTGAAGCACAGGTTGCAGATTTCGGACTTGCAAAGCTAGCTGATGCTGCCAATACGCATGTATCAACCAGGGTTATGGGTACCTTTGGGTACATGGCTCCAGAGTACGCAACAAGCGGAAAATTAACGGATAGATCAGATGTTTTCTCATTTGGGGTTGTCCTCCTTGAGCTTGTAACCGGAAGAAAACCAGTTGATGAAAGTAGGCCATTGGGAGATGAGAGTTTGGTTGAGTGGGCTCGACCACAACTTATCCATGCTTTTGAGACACGCGAGTTTGGTGAATTAGTAGACCCGAGACTAGAAAAACACTACGTGGAGAGTGAAATGTTCAGAATGATAGAGGCAGCTGCAGCTTGTGTTCGCCACTCAGCTCCTAAACGACCTCGTATGTCTCAGGTGGTAAGAGCATTAGACACTGGAGATGAAGTATTCGATTTATCAAACGGGGTGAAATATGGTCAGAGCACTGTTTATGATTCTGGACAGTATGACAAAGACATCATGCTATTCAGGATGATGGGAAATGGCAGCTATGCTGATTCTGAATTATCAACATACAGTAGAGAATCCAGTGTTTCAAGAGAACATTCGAGATCCCATCATGCATGGATGCACAGTGGTTCAAGTACCGAGTCAGAATCAAGAGCTTTCAATAAGCATAGAAACAGTGCTGAATAA

Protein sequence

>Msa1249960

MAPNLNSNSKVSSSSSSSLLSFSSPFLPLFENKKDFGKKKDDKDKGKDKAPSPSKPEPPESSPSHSPPPPKSRSPPKVLPPPPSPPLPSPPPPSPPPNSPPPPSPPPPLLSPPPASPPPPPMPNAQYSASALSSPNSPRKQPPPSLPPPEKSSTSSSSSSPSSSPSPSPSSSSSSSSSSSSSSSSSSSSSTSSKDSPHKAMHAKSGSSHSSGHTPGEIAGYAVAAVLVVAIVAAAVFFAFRKKKAKGDAYVGPYIPPPGANGHYYVQQPSLGNIYGNVNVEGRSTKPLGGGGPLDPAQPISAQIAFTYDMVMEITNAFSSQNVIGQGGFGCVYKGWLKDGKEVAVKTLKAGSGQGDREFRAEVEIISRVHHRHLVSLVGYCISEEQRVLIYEFVPNGNLHHHLHGSGMPVLAWDKRLKIAIGAAKGLAYLHEDCSQKIIHRDIKSANILLDDAFEAQVADFGLAKLADAANTHVSTRVMGTFGYMAPEYATSGKLTDRSDVFSFGVVLLELVTGRKPVDESRPLGDESLVEWARPQLIHAFETREFGELVDPRLEKHYVESEMFRMIEAAAACVRHSAPKRPRMSQVVRALDTGDEVFDLSNGVKYGQSTVYDSGQYDKDIMLFRMMGNGSYADSELSTYSRESSVSREHSRSHHAWMHSGSSTESESRAFNKHRNSAE*