AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1252490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1252490 MtrunA17_Chr8g0368721 85.304 313 40 2 1 308 1 312 4.43e-113 336
Msa1252490 MtrunA17_Chr6g0470151 47.884 378 152 7 1 354 1 357 8.27e-72 232
Msa1252490 MtrunA17_Chr8g0366921 52.147 326 132 5 1 308 1 320 1.36e-66 217
Msa1252490 MtrunA17_Chr2g0289181 44.635 233 68 2 314 485 1 233 2.23e-57 190
Msa1252490 MtrunA17_Chr7g0266621 51.780 309 141 3 1 308 1 302 8.97e-50 172
Msa1252490 MtrunA17_Chr2g0293901 55.491 173 70 4 1 170 1 169 2.34e-34 128
Msa1252490 MtrunA17_Chr1g0202461 53.535 99 44 2 304 402 239 335 5.30e-29 117
Msa1252490 MtrunA17_Chr8g0348311 46.939 98 49 2 304 401 134 228 2.95e-24 103
Msa1252490 MtrunA17_Chr7g0248691 47.917 96 49 1 306 401 133 227 4.30e-24 102
Msa1252490 MtrunA17_Chr2g0293481 45.133 113 50 3 307 409 238 348 1.07e-21 97.8
Msa1252490 MtrunA17_Chr8g0387501 56.944 72 30 1 328 399 130 200 2.67e-21 93.6
Msa1252490 MtrunA17_Chr4g0053281 51.471 68 33 0 328 395 267 334 1.65e-19 89.7
Msa1252490 MtrunA17_Chr5g0401821 51.471 68 33 0 328 395 200 267 3.64e-19 87.4
Msa1252490 MtrunA17_Chr4g0071521 56.716 67 28 1 323 389 242 307 7.34e-19 87.4
Msa1252490 MtrunA17_Chr8g0379461 31.013 158 84 3 325 469 74 219 1.10e-18 85.5
Msa1252490 MtrunA17_Chr2g0319961 56.061 66 29 0 322 387 75 140 1.11e-18 85.9
Msa1252490 MtrunA17_Chr3g0139021 43.158 95 53 1 299 392 285 379 1.86e-18 87.4
Msa1252490 MtrunA17_Chr1g0184691 57.627 59 25 0 328 386 294 352 7.02e-18 85.1
Msa1252490 MtrunA17_Chr7g0270051 55.000 60 27 0 328 387 54 113 1.08e-17 83.2
Msa1252490 MtrunA17_Chr2g0284321 53.968 63 29 0 325 387 33 95 1.79e-17 81.3
Msa1252490 MtrunA17_Chr7g0228941 54.098 61 28 0 328 388 53 113 1.91e-17 78.6
Msa1252490 MtrunA17_Chr1g0147881 47.826 69 36 0 328 396 263 331 1.99e-17 83.6
Msa1252490 MtrunA17_Chr7g0218081 43.373 83 45 2 327 409 410 490 2.02e-15 78.6
Msa1252490 MtrunA17_Chr7g0218081 49.275 69 33 2 323 391 224 290 2.21e-15 78.6
Msa1252490 MtrunA17_Chr7g0263531 55.385 65 27 2 323 387 185 247 2.55e-15 78.2
Msa1252490 MtrunA17_Chr7g0263531 39.604 101 46 2 304 390 324 423 2.64e-15 77.8
Msa1252490 MtrunA17_Chr7g0266611 56.061 66 29 0 382 447 2 67 3.43e-15 73.2
Msa1252490 MtrunA17_Chr7g0243891 51.220 82 35 3 310 391 202 278 5.96e-15 77.4
Msa1252490 MtrunA17_Chr7g0243891 39.189 74 44 1 327 400 374 446 6.62e-12 67.8
Msa1252490 MtrunA17_Chr3g0079691 49.315 73 35 2 325 397 278 348 8.28e-15 77.0
Msa1252490 MtrunA17_Chr3g0079691 43.478 69 38 1 327 395 495 562 1.29e-13 73.6
Msa1252490 MtrunA17_Chr7g0249311 39.048 105 49 3 327 431 392 481 1.40e-14 76.3
Msa1252490 MtrunA17_Chr7g0249311 43.902 82 44 2 317 398 169 248 4.74e-14 74.7
Msa1252490 MtrunA17_Chr4g0042831 43.750 80 44 1 327 406 278 356 1.61e-14 75.1
Msa1252490 MtrunA17_Chr4g0042831 41.176 68 39 1 320 387 138 204 6.99e-11 63.9
Msa1252490 MtrunA17_Chr5g0418191 46.269 67 35 1 327 393 320 385 2.00e-14 75.1
Msa1252490 MtrunA17_Chr5g0418191 35.971 139 75 6 323 458 147 274 7.24e-14 73.6
Msa1252490 MtrunA17_Chr1g0194481 43.662 71 39 1 327 397 158 227 2.60e-14 73.9
Msa1252490 MtrunA17_Chr6g0470161 43.299 97 48 2 396 485 24 120 4.18e-14 68.9
Msa1252490 MtrunA17_Chr4g0074001 37.037 108 55 3 292 387 312 418 5.09e-14 74.3
Msa1252490 MtrunA17_Chr7g0270191 38.542 96 57 2 300 395 119 212 5.96e-14 72.4
Msa1252490 MtrunA17_Chr8g0387981 41.379 87 45 2 302 382 103 189 5.97e-14 73.2
Msa1252490 MtrunA17_Chr5g0443131 50.000 62 30 1 327 388 157 217 1.33e-13 71.6
Msa1252490 MtrunA17_Chr4g0004911 52.174 69 31 2 320 387 176 243 1.34e-13 72.8
Msa1252490 MtrunA17_Chr4g0004911 44.262 61 33 1 327 387 336 395 1.57e-12 69.7
Msa1252490 MtrunA17_Chr4g0051881 49.180 61 30 1 327 387 178 237 1.75e-13 71.6
Msa1252490 MtrunA17_Chr7g0249301 47.619 63 32 1 327 389 292 353 3.78e-13 70.9
Msa1252490 MtrunA17_Chr7g0249301 47.541 61 30 2 327 387 62 120 1.45e-11 66.2
Msa1252490 MtrunA17_Chr3g0106491 50.000 66 31 2 326 391 197 260 4.55e-13 71.2
Msa1252490 MtrunA17_Chr3g0106491 43.548 62 34 1 327 388 365 425 3.00e-11 65.5
Msa1252490 MtrunA17_Chr3g0101131 41.791 67 38 1 327 393 415 480 4.97e-13 71.2
Msa1252490 MtrunA17_Chr3g0101131 48.529 68 33 2 320 387 236 301 1.06e-12 70.5
Msa1252490 MtrunA17_Chr5g0445311 41.791 67 38 1 327 393 364 429 5.29e-13 71.2
Msa1252490 MtrunA17_Chr5g0445311 48.438 64 31 2 324 387 197 258 4.30e-12 68.2
Msa1252490 MtrunA17_Chr3g0129321 44.615 65 35 1 324 388 115 178 6.18e-13 67.8
Msa1252490 MtrunA17_Chr4g0068541 38.571 70 42 1 327 396 208 276 8.24e-13 70.1
Msa1252490 MtrunA17_Chr2g0294231 44.615 65 35 1 327 391 203 266 1.07e-12 69.7
Msa1252490 MtrunA17_Chr5g0432161 53.226 62 27 2 326 387 243 302 1.45e-12 69.7
Msa1252490 MtrunA17_Chr5g0432161 44.444 63 34 1 327 389 421 482 1.60e-12 69.7
Msa1252490 MtrunA17_Chr8g0383251 43.939 66 36 1 322 387 103 167 1.67e-12 66.2
Msa1252490 MtrunA17_Chr5g0418781 40.230 87 50 2 298 384 96 180 2.46e-12 67.4
Msa1252490 MtrunA17_Chr2g0302391 41.667 60 35 0 328 387 212 271 2.53e-12 68.6
Msa1252490 MtrunA17_Chr3g0136961 41.772 79 43 1 327 405 138 213 3.40e-12 67.0
Msa1252490 MtrunA17_Chr3g0126391 37.209 86 45 3 305 382 106 190 4.45e-12 67.4
Msa1252490 MtrunA17_Chr3g0099641 33.708 89 47 1 299 387 235 311 4.96e-12 67.8
Msa1252490 MtrunA17_Chr1g0188941 42.623 61 34 1 327 387 51 110 5.14e-12 62.8
Msa1252490 MtrunA17_Chr7g0238921 48.387 62 25 2 326 384 123 180 5.15e-12 67.0
Msa1252490 MtrunA17_Chr4g0001361 41.026 78 45 1 327 403 162 239 9.23e-12 66.2
Msa1252490 MtrunA17_Chr4g0075201 30.435 115 65 3 275 387 226 327 4.03e-11 65.1
Msa1252490 MtrunA17_Chr5g0447701 46.667 60 31 1 328 387 253 311 4.86e-11 64.3
Msa1252490 MtrunA17_Chr2g0333851 41.935 62 36 0 327 388 169 230 5.56e-11 63.9
Msa1252490 MtrunA17_Chr8g0347251 37.374 99 52 3 294 382 95 193 6.19e-11 63.9
Msa1252490 MtrunA17_Chr1g0189391 42.529 87 46 4 316 400 85 169 6.82e-11 63.5
Msa1252490 MtrunA17_Chr7g0270831 40.000 60 36 0 328 387 217 276 8.52e-11 63.9
Msa1252490 MtrunA17_Chr8g0335811 34.906 106 64 4 289 393 117 218 9.46e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1252490 AT5G52830.1 53.535 99 44 2 304 402 143 239 2.66e-28 115
Msa1252490 AT4G01250.1 36.913 149 83 5 255 401 62 201 4.26e-25 105
Msa1252490 AT4G23550.1 47.872 94 48 1 311 404 124 216 2.15e-22 97.8
Msa1252490 AT5G45050.4 51.613 93 43 2 304 396 1019 1109 2.17e-21 98.6
Msa1252490 AT5G45050.1 51.613 93 43 2 304 396 1158 1248 2.21e-21 98.6
Msa1252490 AT5G45050.2 51.613 93 43 2 304 396 1130 1220 2.77e-21 98.2
Msa1252490 AT2G34830.1 56.522 69 29 1 328 396 216 283 8.97e-20 92.0
Msa1252490 AT1G30650.1 56.522 69 29 1 328 396 218 285 9.42e-20 92.0
Msa1252490 AT2G34830.2 56.522 69 29 1 328 396 225 292 1.02e-19 92.0
Msa1252490 AT4G31550.2 52.308 65 31 0 328 392 246 310 3.27e-19 89.0
Msa1252490 AT4G31550.1 52.308 65 31 0 328 392 247 311 3.41e-19 89.0
Msa1252490 AT2G23320.1 56.140 57 25 0 328 384 241 297 1.92e-18 86.7
Msa1252490 AT2G24570.1 49.231 65 33 0 328 392 244 308 3.02e-18 85.9
Msa1252490 AT5G28650.1 51.515 66 32 0 328 393 263 328 4.55e-18 85.5
Msa1252490 AT1G29280.1 44.086 93 49 1 298 387 42 134 5.63e-18 84.0
Msa1252490 AT3G04670.1 51.515 66 32 0 328 393 263 328 6.55e-18 85.1
Msa1252490 AT4G24240.1 48.810 84 38 2 305 384 256 338 8.72e-18 85.1
Msa1252490 AT3G58710.2 51.471 68 33 0 325 392 68 135 1.16e-17 83.6
Msa1252490 AT3G58710.1 51.471 68 33 0 325 392 69 136 1.26e-17 83.2
Msa1252490 AT3G01080.3 47.436 78 36 3 323 400 201 273 4.10e-17 82.8
Msa1252490 AT3G01080.2 47.436 78 36 3 323 400 201 273 1.36e-16 82.4
Msa1252490 AT3G01080.2 47.541 61 31 1 327 387 344 403 2.90e-14 75.5
Msa1252490 AT3G01080.1 47.436 78 36 3 323 400 163 235 1.58e-16 82.0
Msa1252490 AT3G01080.1 47.541 61 31 1 327 387 306 365 3.81e-14 74.7
Msa1252490 AT2G30590.1 54.237 59 27 0 328 386 314 372 4.78e-16 80.1
Msa1252490 AT2G47260.1 37.391 115 66 3 300 410 148 260 5.78e-15 76.6
Msa1252490 AT2G38470.1 50.000 80 36 3 308 387 167 242 6.50e-15 77.4
Msa1252490 AT2G38470.1 42.466 73 40 2 327 399 362 432 9.99e-13 70.9
Msa1252490 AT2G03340.1 47.761 67 34 1 327 393 415 480 6.58e-15 77.4
Msa1252490 AT2G03340.1 48.571 70 34 2 323 392 246 313 8.53e-14 73.9
Msa1252490 AT3G62340.1 40.476 84 48 2 327 410 118 199 1.41e-14 74.3
Msa1252490 AT4G30935.1 35.780 109 57 3 291 387 283 390 2.95e-14 75.5
Msa1252490 AT2G21900.1 39.362 94 52 2 300 389 78 170 4.10e-14 71.6
Msa1252490 AT1G13960.1 40.816 98 48 4 308 400 205 297 6.00e-14 74.3
Msa1252490 AT1G13960.1 47.541 61 31 1 327 387 409 468 1.83e-13 73.2
Msa1252490 AT5G56270.1 34.194 155 78 5 325 457 271 423 6.24e-14 74.7
Msa1252490 AT5G56270.1 44.444 63 34 1 327 389 487 548 9.31e-13 71.2
Msa1252490 AT4G26440.1 45.946 74 38 2 325 398 176 247 7.10e-14 74.3
Msa1252490 AT4G26440.1 37.805 82 50 1 327 408 372 452 9.71e-12 67.8
Msa1252490 AT1G13960.2 47.436 78 36 3 323 400 72 144 1.31e-13 72.8
Msa1252490 AT1G13960.2 47.541 61 31 1 327 387 256 315 3.30e-13 71.2
Msa1252490 AT1G64000.1 38.095 84 48 3 302 384 90 170 1.84e-13 69.7
Msa1252490 AT2G30250.1 50.000 60 29 1 328 387 329 387 1.85e-13 72.4
Msa1252490 AT2G30250.1 48.387 62 30 2 326 387 165 224 1.11e-11 67.0
Msa1252490 AT5G43290.1 55.172 58 25 1 327 384 114 170 2.52e-13 70.9
Msa1252490 AT5G41570.1 40.000 85 48 3 302 384 71 154 5.52e-13 67.8
Msa1252490 AT5G07100.1 35.455 110 64 3 326 429 116 224 6.07e-13 70.1
Msa1252490 AT5G07100.1 40.580 69 40 1 327 395 234 301 2.73e-12 68.2
Msa1252490 AT2G37260.1 38.095 84 48 2 304 384 323 405 1.00e-12 70.5
Msa1252490 AT2G37260.1 49.254 67 32 2 323 389 161 225 5.44e-12 68.2
Msa1252490 AT2G04880.1 48.333 60 30 1 328 387 308 366 1.35e-12 70.1
Msa1252490 AT1G69310.4 39.773 88 51 2 298 385 119 204 1.38e-12 68.6
Msa1252490 AT1G69310.2 39.773 88 51 2 298 385 119 204 1.38e-12 68.6
Msa1252490 AT1G69310.1 39.773 88 51 2 298 385 119 204 1.38e-12 68.6
Msa1252490 AT2G04880.2 48.333 60 30 1 328 387 284 342 1.47e-12 70.1
Msa1252490 AT2G37260.3 38.095 84 48 2 304 384 243 325 1.50e-12 69.3
Msa1252490 AT2G37260.3 49.254 67 32 2 323 389 81 145 8.62e-12 67.0
Msa1252490 AT1G80840.1 43.548 62 35 0 327 388 146 207 1.50e-12 68.9
Msa1252490 AT2G37260.2 38.095 84 48 2 304 384 241 323 1.79e-12 68.9
Msa1252490 AT2G37260.2 49.254 67 32 2 323 389 79 143 1.01e-11 66.6
Msa1252490 AT2G40740.1 37.931 87 49 2 301 382 144 230 1.80e-12 68.6
Msa1252490 AT1G29860.1 43.478 69 38 1 327 395 136 203 1.87e-12 68.2
Msa1252490 AT1G55600.1 46.970 66 33 2 322 387 303 366 2.13e-12 69.7
Msa1252490 AT2G40740.3 37.931 87 49 2 301 382 153 239 2.21e-12 68.2
Msa1252490 AT5G07100.2 35.455 110 64 3 326 429 23 131 2.51e-12 66.6
Msa1252490 AT5G07100.2 44.262 61 33 1 327 387 141 200 8.33e-12 65.1
Msa1252490 AT5G07100.4 35.455 110 64 3 326 429 23 131 2.51e-12 66.6
Msa1252490 AT5G07100.4 44.262 61 33 1 327 387 141 200 8.33e-12 65.1
Msa1252490 AT5G07100.5 35.455 110 64 3 326 429 23 131 2.51e-12 66.6
Msa1252490 AT5G07100.5 44.262 61 33 1 327 387 141 200 8.33e-12 65.1
Msa1252490 AT5G07100.3 35.455 110 64 3 326 429 23 131 2.51e-12 66.6
Msa1252490 AT5G07100.3 44.262 61 33 1 327 387 141 200 8.33e-12 65.1
Msa1252490 AT4G18170.1 41.026 78 44 2 307 384 153 228 2.70e-12 68.2
Msa1252490 AT5G26170.1 47.619 63 32 1 322 384 108 169 2.85e-12 65.5
Msa1252490 AT4G26640.7 38.462 104 57 5 326 426 170 269 4.61e-12 68.6
Msa1252490 AT4G26640.7 40.984 61 35 1 327 387 341 400 1.76e-11 66.6
Msa1252490 AT4G26640.2 38.462 104 57 5 326 426 210 309 4.86e-12 68.6
Msa1252490 AT4G26640.2 40.984 61 35 1 327 387 381 440 1.80e-11 67.0
Msa1252490 AT5G45260.1 41.026 78 43 2 328 403 1209 1285 5.06e-12 68.9
Msa1252490 AT4G26640.1 38.462 104 57 5 326 426 138 237 6.53e-12 68.2
Msa1252490 AT4G26640.1 40.984 61 35 1 327 387 309 368 2.33e-11 66.2
Msa1252490 AT5G49520.1 44.828 58 31 1 327 384 221 277 7.51e-12 67.4
Msa1252490 AT4G26640.5 38.462 104 57 5 326 426 34 133 8.82e-12 67.0
Msa1252490 AT4G26640.5 40.984 61 35 1 327 387 205 264 2.26e-11 65.9
Msa1252490 AT4G26640.6 38.462 104 57 5 326 426 34 133 8.82e-12 67.0
Msa1252490 AT4G26640.6 40.984 61 35 1 327 387 205 264 2.26e-11 65.9
Msa1252490 AT4G26640.4 38.462 104 57 5 326 426 34 133 8.82e-12 67.0
Msa1252490 AT4G26640.4 40.984 61 35 1 327 387 205 264 2.26e-11 65.9
Msa1252490 AT4G26640.3 38.462 104 57 5 326 426 34 133 8.82e-12 67.0
Msa1252490 AT4G26640.3 40.984 61 35 1 327 387 205 264 2.26e-11 65.9
Msa1252490 AT2G46400.1 43.182 88 45 3 300 382 74 161 1.30e-11 65.9
Msa1252490 AT5G46350.1 41.667 72 40 2 313 384 170 239 1.62e-11 65.9
Msa1252490 AT2G44745.1 34.694 98 62 2 288 384 105 201 2.36e-11 63.9
Msa1252490 AT3G01970.1 40.845 71 39 2 327 395 65 134 2.81e-11 62.0
Msa1252490 AT4G12020.6 32.800 125 74 5 294 412 430 550 5.96e-11 65.9
Msa1252490 AT4G12020.8 32.800 125 74 5 294 412 430 550 5.96e-11 65.9
Msa1252490 AT4G22070.4 40.351 57 34 0 328 384 298 354 9.34e-11 64.7
Msa1252490 AT4G22070.3 40.351 57 34 0 328 384 298 354 9.34e-11 64.7
Msa1252490 AT4G22070.2 40.351 57 34 0 328 384 298 354 9.34e-11 64.7
Msa1252490 AT4G22070.1 40.351 57 34 0 328 384 298 354 9.34e-11 64.7

Find 136 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGGCTTAAGTGGGGTAAT+TGG 0.193333 8_2:-49438851 None:intergenic
ACTACAAATGTAGAAGTTTC+TGG 0.203501 8_2:+49441557 Msa1252490:CDS
AGTCTTTGATGACTTGTTTC+GGG 0.228480 8_2:-49441704 None:intergenic
GGTAGTGATGGTTGGGATAC+TGG 0.266171 8_2:-49438945 None:intergenic
GGAAGGGATGGTGGTGCTTG+AGG 0.267804 8_2:-49438990 None:intergenic
TCGGCTTAAGTGGGGTAATT+GGG 0.268846 8_2:-49438850 None:intergenic
GAAGGGATGGTGGTGCTTGA+GGG 0.285161 8_2:-49438989 None:intergenic
TGGATGTGGAGACCCTTTAA+TGG 0.287579 8_2:-49439763 None:intergenic
TGGAGGTGGAGTTTCAAAAT+TGG 0.294278 8_2:-49438732 None:intergenic
TTGAGGGGATATGATGTTAT+TGG 0.296773 8_2:-49438771 None:intergenic
AACGGCAACATCGCCATATT+AGG 0.297130 8_2:-49441784 None:intergenic
TGCGATTTCACTGCGGGCAT+GGG 0.316131 8_2:-49439407 None:intergenic
TAGTGAAGATGGTGGTAGTG+GGG 0.325049 8_2:-49439201 None:intergenic
AAACTTGGAATCGCCTAATA+TGG 0.330985 8_2:+49441771 Msa1252490:CDS
AGGGTGGCGAATGTGGTTGT+AGG 0.331360 8_2:-49439226 None:intergenic
CTCCAATGGACAATGATGAT+TGG 0.334060 8_2:+49438654 None:intergenic
CAAAATTTAAATCAAGAAAA+AGG 0.335426 8_2:+49439560 Msa1252490:CDS
TAGTCTTTGATGACTTGTTT+CGG 0.341304 8_2:-49441705 None:intergenic
GTAGTGATGGTAGGTATGAT+AGG 0.345611 8_2:-49439262 None:intergenic
GTAGTTGATTGCGCTTTCTC+AGG 0.345960 8_2:-49439480 None:intergenic
ACTTATGTTGTGACATATAA+AGG 0.361618 8_2:+49441628 Msa1252490:CDS
GGATATGATGTTATTGGTTG+TGG 0.366736 8_2:-49438765 None:intergenic
TTGCGATTTCACTGCGGGCA+TGG 0.375796 8_2:-49439408 None:intergenic
TACACTTCATTGTTACGGTT+TGG 0.377794 8_2:-49439373 None:intergenic
AGTTCTTACCGATACGTCTA+TGG 0.380944 8_2:+49441834 Msa1252490:CDS
ATGAAATCATTCGGCTTAAG+TGG 0.384871 8_2:-49438860 None:intergenic
AGGGATGGTGGTGCTTGAGG+GGG 0.385352 8_2:-49438987 None:intergenic
GGATGTGGAGACCCTTTAAT+GGG 0.385486 8_2:-49439762 None:intergenic
GTTGGGGTGGTAGTGATGGT+TGG 0.392907 8_2:-49438953 None:intergenic
GTTGTGGTGGTTAGAGTTGG+AGG 0.394427 8_2:-49438749 None:intergenic
GGTAGTGAAGATGGTGGTAG+TGG 0.395152 8_2:-49439203 None:intergenic
TTTGTTCGAGGTAGTAGAAA+TGG 0.396886 8_2:-49439502 None:intergenic
AAGGGATGGTGGTGCTTGAG+GGG 0.398824 8_2:-49438988 None:intergenic
AAACATGACACCTATGTTAG+AGG 0.407154 8_2:+49441897 Msa1252490:CDS
AGGGGTTGTTGTGGAATTGA+TGG 0.409578 8_2:-49439460 None:intergenic
AGAGGTGGGGATAGTGGTGT+TGG 0.411378 8_2:-49439161 None:intergenic
TCCAATGGACAATGATGATT+GGG 0.413894 8_2:+49438655 None:intergenic
TTGGTTGTGGTGGTTAGAGT+TGG 0.415176 8_2:-49438752 None:intergenic
CGCTTTCTCAGGGGTTGTTG+TGG 0.415304 8_2:-49439469 None:intergenic
GTTGGGATACTGGTGATAGT+AGG 0.415858 8_2:-49438935 None:intergenic
TTGGGGTGGTAGTGATGGTT+GGG 0.421087 8_2:-49438952 None:intergenic
CTACAAATGTAGAAGTTTCT+GGG 0.423709 8_2:+49441558 Msa1252490:CDS
TAGAAGTTTCTGGGATTGTT+CGG 0.428728 8_2:+49441567 Msa1252490:CDS
GGGTGGCGAATGTGGTTGTA+GGG 0.432234 8_2:-49439225 None:intergenic
GATGGTAGAGGTGGGGATAG+TGG 0.437887 8_2:-49439167 None:intergenic
TCAAGCTCTATGAAATCATT+CGG 0.442212 8_2:-49438869 None:intergenic
GGTAATGTGGGAAGGGATGG+TGG 0.443946 8_2:-49438999 None:intergenic
GATAGTAGGGGTGGGGATAG+TGG 0.445675 8_2:-49438921 None:intergenic
AGTGGTTGGGGTGGTAGTGA+TGG 0.446309 8_2:-49438957 None:intergenic
ACTTGAGCCTCGTTGCTCTC+TGG 0.448771 8_2:-49441814 None:intergenic
ACGAGCCTTCAGAAGATTCA+TGG 0.450159 8_2:+49439715 Msa1252490:CDS
TATGTTAGAGGATCTCTTGA+TGG 0.450163 8_2:+49441909 Msa1252490:CDS
TTGGGATACTGGTGATAGTA+GGG 0.453252 8_2:-49438934 None:intergenic
GAGGTGGGGATAGTGGTGTT+GGG 0.454610 8_2:-49439160 None:intergenic
AAACATACACTTCATTGTTA+CGG 0.454894 8_2:-49439378 None:intergenic
GGGGTGGGGATAGTGGTTGT+TGG 0.455018 8_2:-49438914 None:intergenic
TAGTGATGGTAGGTATGATA+GGG 0.457142 8_2:-49439261 None:intergenic
TGGGGCTGAGTTATTCTCCT+TGG 0.469030 8_2:-49438831 None:intergenic
TTAATCAGAACTCTGTTGTT+GGG 0.469555 8_2:+49441677 Msa1252490:CDS
TGTTGGGAAATTAGAAAAGG+TGG 0.469828 8_2:-49439068 None:intergenic
AGGGGTAGTGGTGGTGAGGG+TGG 0.470018 8_2:-49439242 None:intergenic
TCATCAAAGCATGGAGGTGT+AGG 0.470833 8_2:-49438797 None:intergenic
AGTGAAGATGGTGGTAGTGG+GGG 0.472105 8_2:-49439200 None:intergenic
GTTAATCAGAACTCTGTTGT+TGG 0.473025 8_2:+49441676 Msa1252490:CDS
GGTGGTGAGGGTGGCGAATG+TGG 0.473258 8_2:-49439233 None:intergenic
TTATGCATTGGCAAAGAAGC+TGG 0.473516 8_2:+49441730 Msa1252490:CDS
GTAGTGAAGATGGTGGTAGT+GGG 0.473726 8_2:-49439202 None:intergenic
GGAATTGATGGATGGTTGAA+TGG 0.474434 8_2:-49439448 None:intergenic
GGTGGTAATGTGGGAAGGGA+TGG 0.475750 8_2:-49439002 None:intergenic
GAATGGACAATCAAAATTGT+TGG 0.476039 8_2:-49439431 None:intergenic
TGATAGGGGTAGTGGTGGTG+AGG 0.478349 8_2:-49439246 None:intergenic
TAGTTGATTGCGCTTTCTCA+GGG 0.481392 8_2:-49439479 None:intergenic
TTGCGCAAATGTCAGAAACT+TGG 0.482429 8_2:+49441756 Msa1252490:CDS
TATGATGTTATTGGTTGTGG+TGG 0.483931 8_2:-49438762 None:intergenic
TCTAACATAGGTGTCATGTT+TGG 0.484082 8_2:-49441895 None:intergenic
GTTGGTTGCGATTTCACTGC+GGG 0.484377 8_2:-49439413 None:intergenic
GGTTGTAGGGGTAGTGAAGA+TGG 0.488717 8_2:-49439212 None:intergenic
TACTGGTGATAGTAGGGGTG+GGG 0.488721 8_2:-49438928 None:intergenic
GTTGTTGTTCGATTTGTGTT+GGG 0.488863 8_2:-49439084 None:intergenic
GGTAGGAGTAGTGGTTGGGG+TGG 0.493221 8_2:-49438966 None:intergenic
TGTTGTTGTTCGATTTGTGT+TGG 0.497681 8_2:-49439085 None:intergenic
ATAGAGCACCACTATTGATC+TGG 0.499970 8_2:-49441939 None:intergenic
GTGGTGGTTAGAGTTGGAGG+TGG 0.500554 8_2:-49438746 None:intergenic
ACAGAGTTCTGATTAACTGC+TGG 0.506512 8_2:-49441670 None:intergenic
GATACTGGTGATAGTAGGGG+TGG 0.507596 8_2:-49438930 None:intergenic
TTGCTCTCTGGCTGATCTAA+CGG 0.509856 8_2:-49441802 None:intergenic
GGTGGTAGGAGTAGCAAAAG+TGG 0.510553 8_2:-49438711 None:intergenic
GTTGTTGTGGAATTGATGGA+TGG 0.514803 8_2:-49439456 None:intergenic
TTGTGTTGGGAAATTAGAAA+AGG 0.515655 8_2:-49439071 None:intergenic
TGATGGTATTGGTGGTAATG+TGG 0.520234 8_2:-49439012 None:intergenic
CGGCTTAAGTGGGGTAATTG+GGG 0.520319 8_2:-49438849 None:intergenic
GATAGGGGTAGTGGTGGTGA+GGG 0.522610 8_2:-49439245 None:intergenic
AGGTGGAGTTTCAAAATTGG+TGG 0.528008 8_2:-49438729 None:intergenic
GGTGTGAGCAGTGATGGTAG+AGG 0.528684 8_2:-49439179 None:intergenic
GTGAGCAGTGATGGTAGAGG+TGG 0.533809 8_2:-49439176 None:intergenic
ATCAAGAGATCCTCTAACAT+AGG 0.542974 8_2:-49441907 None:intergenic
TGAGCAGTGATGGTAGAGGT+GGG 0.544379 8_2:-49439175 None:intergenic
ATTAGTCGTTGTAGTAGTGA+TGG 0.545537 8_2:-49439275 None:intergenic
GGTAGGTATGATAGGGGTAG+TGG 0.546289 8_2:-49439254 None:intergenic
TGAAATCATTCGGCTTAAGT+GGG 0.547986 8_2:-49438859 None:intergenic
GATGGTATTGGTGGTAATGT+GGG 0.548603 8_2:-49439011 None:intergenic
GACTTCAACCAGATCAATAG+TGG 0.548651 8_2:+49441931 Msa1252490:CDS
TCCCAATCATCATTGTCCAT+TGG 0.550956 8_2:-49438656 None:intergenic
CATGCCCATGAATCTTCTGA+AGG 0.555237 8_2:-49439720 None:intergenic
CATCAAAGCATGGAGGTGTA+GGG 0.555900 8_2:-49438796 None:intergenic
ACTAGTGTTAGTGATGGTAT+TGG 0.562993 8_2:-49439023 None:intergenic
GGAGTTTCAAAATTGGTGGT+AGG 0.564205 8_2:-49438725 None:intergenic
GGTGGCGAATGTGGTTGTAG+GGG 0.565157 8_2:-49439224 None:intergenic
AGGTGGGGATAGTGGTGTTG+GGG 0.565500 8_2:-49439159 None:intergenic
CGAGCCTTCAGAAGATTCAT+GGG 0.566390 8_2:+49439716 Msa1252490:CDS
AGTGATGGTAGGTATGATAG+GGG 0.566864 8_2:-49439260 None:intergenic
CATGGCACACAAATGTCACT+TGG 0.574104 8_2:-49439683 None:intergenic
TTAAAGGGTCTCCACATCCA+AGG 0.584498 8_2:+49439766 Msa1252490:CDS
AAACTAAAATCATCAAAGCA+TGG 0.588328 8_2:-49438806 None:intergenic
TCAAAGACTAGATTATGCAT+TGG 0.588584 8_2:+49441718 Msa1252490:CDS
ATGAAAACTAGTGTTAGTGA+TGG 0.596957 8_2:-49439029 None:intergenic
AAGGCTCGTCTTCCCTCACA+TGG 0.597848 8_2:-49439701 None:intergenic
CACAACATAAGTGTTCTCAC+TGG 0.597849 8_2:-49441617 None:intergenic
CTTCAGAAGATTCATGGGCA+TGG 0.598976 8_2:+49439721 Msa1252490:CDS
ATACTGGTGATAGTAGGGGT+GGG 0.601151 8_2:-49438929 None:intergenic
AGTGTTAGTGATGGTATTGG+TGG 0.606164 8_2:-49439020 None:intergenic
GTATTGGTGGTAATGTGGGA+AGG 0.610190 8_2:-49439007 None:intergenic
TATTGGTGGTAATGTGGGAA+GGG 0.622832 8_2:-49439006 None:intergenic
GACATTTGTGTGCCATGTGA+GGG 0.624856 8_2:+49439689 Msa1252490:CDS
CTTTGATGACTTGTTTCGGG+AGG 0.626610 8_2:-49441701 None:intergenic
TGGGATACTGGTGATAGTAG+GGG 0.637481 8_2:-49438933 None:intergenic
GTCGTTGTAGTAGTGATGGT+AGG 0.641331 8_2:-49439271 None:intergenic
TGACATTTGTGTGCCATGTG+AGG 0.643817 8_2:+49439688 Msa1252490:CDS
AGGTATGATAGGGGTAGTGG+TGG 0.649146 8_2:-49439251 None:intergenic
GAAATCATTCGGCTTAAGTG+GGG 0.658767 8_2:-49438858 None:intergenic
AGTTGATTGCGCTTTCTCAG+GGG 0.662828 8_2:-49439478 None:intergenic
TGTAGGGGTAGTGAAGATGG+TGG 0.689397 8_2:-49439209 None:intergenic
GAGCAGTGATGGTAGAGGTG+GGG 0.706368 8_2:-49439174 None:intergenic
TGTTGGTTGCGATTTCACTG+CGG 0.712957 8_2:-49439414 None:intergenic
CTAAAATCATCAAAGCATGG+AGG 0.732238 8_2:-49438803 None:intergenic
AGATCAGCCAGAGAGCAACG+AGG 0.772309 8_2:+49441807 Msa1252490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATATAATAAATAAT+AGG - chr8_2:49439599-49439618 None:intergenic 0.0%
!! AAATTTATTTATAGTGAAAT+TGG + chr8_2:49440301-49440320 Msa1252490:intron 10.0%
!!! GATTTAAATTTTGGTTTTTT+CGG - chr8_2:49439553-49439572 None:intergenic 15.0%
!!! CTTTTTCTTGATTTAAATTT+TGG - chr8_2:49439562-49439581 None:intergenic 15.0%
!! CAAAATTTAAATCAAGAAAA+AGG + chr8_2:49439560-49439579 Msa1252490:CDS 15.0%
!!! ATGAAATGTATAATGATTTT+AGG - chr8_2:49439641-49439660 None:intergenic 15.0%
!!! TTCACTATAAATAAATTTTG+CGG - chr8_2:49440299-49440318 None:intergenic 15.0%
!! TATCAAAATAAGGATTAAAA+TGG - chr8_2:49440339-49440358 None:intergenic 15.0%
!!! GATTTTATTCTATCAAAATA+AGG - chr8_2:49440349-49440368 None:intergenic 15.0%
!! TAATATATACATTTATATGC+AGG + chr8_2:49440827-49440846 Msa1252490:intron 15.0%
!! AATATATACATTTATATGCA+GGG + chr8_2:49440828-49440847 Msa1252490:intron 15.0%
!! AAAGACTAAAATATTATATG+CGG - chr8_2:49440883-49440902 None:intergenic 15.0%
!!! TAGATATTTTATAGTTTTAC+TGG - chr8_2:49441006-49441025 None:intergenic 15.0%
!! AGTAAAACTATAAAATATCT+AGG + chr8_2:49441005-49441024 Msa1252490:intron 15.0%
!! GTAAAACTATAAAATATCTA+GGG + chr8_2:49441006-49441025 Msa1252490:intron 15.0%
!! TGAATATTTAAATGATTGTT+TGG - chr8_2:49441223-49441242 None:intergenic 15.0%
!!! AAATATACCACTTTTTCTTT+TGG - chr8_2:49440445-49440464 None:intergenic 20.0%
!!! GTGGTATATTTTGTAAAAAA+AGG + chr8_2:49440454-49440473 Msa1252490:intron 20.0%
!!! AATGAAGTGAGATTTTTTTT+TGG - chr8_2:49440719-49440738 None:intergenic 20.0%
! AAACTAAAATCATCAAAGCA+TGG - chr8_2:49438809-49438828 None:intergenic 25.0%
!!! TGATGATTTTAGTTTTACCA+AGG + chr8_2:49438814-49438833 Msa1252490:CDS 25.0%
! AAACATACACTTCATTGTTA+CGG - chr8_2:49439381-49439400 None:intergenic 25.0%
!! AAAAATCTAGAATTTGTTCG+AGG - chr8_2:49439517-49439536 None:intergenic 25.0%
! ATGTAACAAAATTCTTCCAA+TGG - chr8_2:49439920-49439939 None:intergenic 25.0%
!! CACTATTTAAAACTCTAGTT+GGG - chr8_2:49439953-49439972 None:intergenic 25.0%
!! TCACTATTTAAAACTCTAGT+TGG - chr8_2:49439954-49439973 None:intergenic 25.0%
!!! CTAGAGTTTTAAATAGTGAT+CGG + chr8_2:49439955-49439974 Msa1252490:intron 25.0%
! TTAACTACAATTCTTTCCAA+TGG - chr8_2:49439998-49440017 None:intergenic 25.0%
!!! ATGTACTGTTAATTGCTTTT+TGG + chr8_2:49440160-49440179 Msa1252490:intron 25.0%
! GGATTAAAATGGATATTGAA+GGG - chr8_2:49440328-49440347 None:intergenic 25.0%
! AGGATTAAAATGGATATTGA+AGG - chr8_2:49440329-49440348 None:intergenic 25.0%
! CTAATATGAATAAAGTTTGC+AGG + chr8_2:49440398-49440417 Msa1252490:intron 25.0%
! ATAAAGACCAAAAGAAAAAG+TGG + chr8_2:49440435-49440454 Msa1252490:intron 25.0%
! AAAAGAGAGGATATATTTGT+AGG + chr8_2:49440486-49440505 Msa1252490:intron 25.0%
! AAAGAGAGGATATATTTGTA+GGG + chr8_2:49440487-49440506 Msa1252490:intron 25.0%
!!! AACGTGAAAGATATTGATTT+AGG - chr8_2:49440671-49440690 None:intergenic 25.0%
! TACATCATATTGTTCTATGT+GGG + chr8_2:49440938-49440957 Msa1252490:intron 25.0%
!!! TTTTTTTCTTTCCATGTACT+AGG - chr8_2:49441391-49441410 None:intergenic 25.0%
! AAAAAATTCTAAGTCAAGTG+AGG + chr8_2:49441407-49441426 Msa1252490:intron 25.0%
! AAAAATTCTAAGTCAAGTGA+GGG + chr8_2:49441408-49441427 Msa1252490:intron 25.0%
! AAACCATACACATATAAACA+GGG - chr8_2:49441467-49441486 None:intergenic 25.0%
! AAAACCATACACATATAAAC+AGG - chr8_2:49441468-49441487 None:intergenic 25.0%
! TATTAAACAGTACGATTTAG+CGG + chr8_2:49441491-49441510 Msa1252490:intron 25.0%
!! AAATCGATGAATGATTTTGT+AGG + chr8_2:49441530-49441549 Msa1252490:intron 25.0%
! ACTTATGTTGTGACATATAA+AGG + chr8_2:49441628-49441647 Msa1252490:CDS 25.0%
! ATCTCTTTTCTATTGTTCGT+AGG + chr8_2:49438678-49438697 Msa1252490:CDS 30.0%
TCAAGCTCTATGAAATCATT+CGG - chr8_2:49438872-49438891 None:intergenic 30.0%
ATGAAAACTAGTGTTAGTGA+TGG - chr8_2:49439032-49439051 None:intergenic 30.0%
!! AGAAAAGGTGGAATTTTGAT+TGG - chr8_2:49439059-49439078 None:intergenic 30.0%
TTGTGTTGGGAAATTAGAAA+AGG - chr8_2:49439074-49439093 None:intergenic 30.0%
GTTTACTGAGCTAGAAAAAA+TGG + chr8_2:49439123-49439142 Msa1252490:CDS 30.0%
GAATGGACAATCAAAATTGT+TGG - chr8_2:49439434-49439453 None:intergenic 30.0%
TATGGAGAAAAACCCATTAA+AGG + chr8_2:49439750-49439769 Msa1252490:CDS 30.0%
ATGGAGAAAAACCCATTAAA+GGG + chr8_2:49439751-49439770 Msa1252490:CDS 30.0%
CGTTTAATGCATAAAAACCT+TGG - chr8_2:49439786-49439805 None:intergenic 30.0%
! CACTATTTAAAACTCTAGGT+GGG - chr8_2:49439875-49439894 None:intergenic 30.0%
ACAAAATTCTTCCAATGGTA+AGG - chr8_2:49439915-49439934 None:intergenic 30.0%
!!! AGTTTTAAATAGTGATCGGA+AGG + chr8_2:49439959-49439978 Msa1252490:intron 30.0%
TACAATTCTTTCCAATGGTA+AGG - chr8_2:49439993-49440012 None:intergenic 30.0%
CAAAAAATGTTACATCTGCA+TGG - chr8_2:49440203-49440222 None:intergenic 30.0%
TGTGTTTCCTAAAAAACAGA+GGG - chr8_2:49440234-49440253 None:intergenic 30.0%
TTGTGTTTCCTAAAAAACAG+AGG - chr8_2:49440235-49440254 None:intergenic 30.0%
ACAATTACACCTTTGATCTT+TGG + chr8_2:49440251-49440270 Msa1252490:intron 30.0%
AAGGAAGAAAAAGAAAAGAG+AGG + chr8_2:49440473-49440492 Msa1252490:intron 30.0%
AAGAGAGGATATATTTGTAG+GGG + chr8_2:49440488-49440507 Msa1252490:intron 30.0%
TATACATTTATATGCAGGGA+CGG + chr8_2:49440832-49440851 Msa1252490:intron 30.0%
GTACATCATATTGTTCTATG+TGG + chr8_2:49440937-49440956 Msa1252490:intron 30.0%
! TAAAATATCTAGGGTGCAAT+TGG + chr8_2:49441015-49441034 Msa1252490:intron 30.0%
GTGAATAAGTAAGGAATTCA+GGG - chr8_2:49441077-49441096 None:intergenic 30.0%
TTCCTTACTTATTCACCTTA+AGG + chr8_2:49441081-49441100 Msa1252490:intron 30.0%
TCCTTACTTATTCACCTTAA+GGG + chr8_2:49441082-49441101 Msa1252490:intron 30.0%
TGACTAAATATTCACCCTTA+AGG - chr8_2:49441099-49441118 None:intergenic 30.0%
ACTACAAATGTAGAAGTTTC+TGG + chr8_2:49441557-49441576 Msa1252490:CDS 30.0%
CTACAAATGTAGAAGTTTCT+GGG + chr8_2:49441558-49441577 Msa1252490:CDS 30.0%
TTAATCAGAACTCTGTTGTT+GGG + chr8_2:49441677-49441696 Msa1252490:CDS 30.0%
!! TAGTCTTTGATGACTTGTTT+CGG - chr8_2:49441708-49441727 None:intergenic 30.0%
TCAAAGACTAGATTATGCAT+TGG + chr8_2:49441718-49441737 Msa1252490:CDS 30.0%
! TATGATGTTATTGGTTGTGG+TGG - chr8_2:49438765-49438784 None:intergenic 35.0%
! GGATATGATGTTATTGGTTG+TGG - chr8_2:49438768-49438787 None:intergenic 35.0%
TTGAGGGGATATGATGTTAT+TGG - chr8_2:49438774-49438793 None:intergenic 35.0%
CTAAAATCATCAAAGCATGG+AGG - chr8_2:49438806-49438825 None:intergenic 35.0%
TGAAATCATTCGGCTTAAGT+GGG - chr8_2:49438862-49438881 None:intergenic 35.0%
ATGAAATCATTCGGCTTAAG+TGG - chr8_2:49438863-49438882 None:intergenic 35.0%
ACTAGTGTTAGTGATGGTAT+TGG - chr8_2:49439026-49439045 None:intergenic 35.0%
TGTTGGGAAATTAGAAAAGG+TGG - chr8_2:49439071-49439090 None:intergenic 35.0%
! GTTGTTGTTCGATTTGTGTT+GGG - chr8_2:49439087-49439106 None:intergenic 35.0%
! TGTTGTTGTTCGATTTGTGT+TGG - chr8_2:49439088-49439107 None:intergenic 35.0%
TAGTGATGGTAGGTATGATA+GGG - chr8_2:49439264-49439283 None:intergenic 35.0%
ATTAGTCGTTGTAGTAGTGA+TGG - chr8_2:49439278-49439297 None:intergenic 35.0%
TACACTTCATTGTTACGGTT+TGG - chr8_2:49439376-49439395 None:intergenic 35.0%
TTTGTTCGAGGTAGTAGAAA+TGG - chr8_2:49439505-49439524 None:intergenic 35.0%
!!! GGTTTTTTCGGATGTGTATT+TGG - chr8_2:49439541-49439560 None:intergenic 35.0%
TCATGGGCATGGAAAAAATA+TGG + chr8_2:49439732-49439751 Msa1252490:CDS 35.0%
ATGCATAAAAACCTTGGATG+TGG - chr8_2:49439780-49439799 None:intergenic 35.0%
! CCACTATTTAAAACTCTAGG+TGG - chr8_2:49439876-49439895 None:intergenic 35.0%
!!! CCACCTAGAGTTTTAAATAG+TGG + chr8_2:49439873-49439892 Msa1252490:intron 35.0%
! CGACCACTATTTAAAACTCT+AGG - chr8_2:49439879-49439898 None:intergenic 35.0%
GTAGTTAAATGCAACATACG+TGG + chr8_2:49440010-49440029 Msa1252490:intron 35.0%
!!! GTTTAGTCCCTCTGTTTTTT+AGG + chr8_2:49440224-49440243 Msa1252490:intron 35.0%
CATCAAAGACCAAAGATCAA+AGG - chr8_2:49440263-49440282 None:intergenic 35.0%
TCTTACACTGAGTGACATAT+TGG - chr8_2:49440777-49440796 None:intergenic 35.0%
ATATGTCACTCAGTGTAAGA+CGG + chr8_2:49440777-49440796 Msa1252490:intron 35.0%
TAGGAACATTGCGATATACA+AGG + chr8_2:49441038-49441057 Msa1252490:intron 35.0%
AGGAACATTGCGATATACAA+GGG + chr8_2:49441039-49441058 Msa1252490:intron 35.0%
GGTGAATAAGTAAGGAATTC+AGG - chr8_2:49441078-49441097 None:intergenic 35.0%
ACCCTTAAGGTGAATAAGTA+AGG - chr8_2:49441086-49441105 None:intergenic 35.0%
CATTTCTTGCTAATGTCTCT+CGG - chr8_2:49441291-49441310 None:intergenic 35.0%
ACTAGGACATATCAAAGCAT+GGG - chr8_2:49441374-49441393 None:intergenic 35.0%
TACTAGGACATATCAAAGCA+TGG - chr8_2:49441375-49441394 None:intergenic 35.0%
CTTTGATATGTCCTAGTACA+TGG + chr8_2:49441377-49441396 Msa1252490:intron 35.0%
CATACACATATAAACAGGGA+CGG - chr8_2:49441463-49441482 None:intergenic 35.0%
!! TAGAAGTTTCTGGGATTGTT+CGG + chr8_2:49441567-49441586 Msa1252490:CDS 35.0%
GTTAATCAGAACTCTGTTGT+TGG + chr8_2:49441676-49441695 Msa1252490:CDS 35.0%
!! AGTCTTTGATGACTTGTTTC+GGG - chr8_2:49441707-49441726 None:intergenic 35.0%
AAACTTGGAATCGCCTAATA+TGG + chr8_2:49441771-49441790 Msa1252490:CDS 35.0%
CAAAAACTCCATAGACGTAT+CGG - chr8_2:49441845-49441864 None:intergenic 35.0%
! GATACGTCTATGGAGTTTTT+GGG + chr8_2:49441844-49441863 Msa1252490:CDS 35.0%
! TCTAACATAGGTGTCATGTT+TGG - chr8_2:49441898-49441917 None:intergenic 35.0%
AAACATGACACCTATGTTAG+AGG + chr8_2:49441897-49441916 Msa1252490:CDS 35.0%
ATCAAGAGATCCTCTAACAT+AGG - chr8_2:49441910-49441929 None:intergenic 35.0%
TATGTTAGAGGATCTCTTGA+TGG + chr8_2:49441909-49441928 Msa1252490:CDS 35.0%
! GGAGTTTCAAAATTGGTGGT+AGG - chr8_2:49438728-49438747 None:intergenic 40.0%
AGGTGGAGTTTCAAAATTGG+TGG - chr8_2:49438732-49438751 None:intergenic 40.0%
TGGAGGTGGAGTTTCAAAAT+TGG - chr8_2:49438735-49438754 None:intergenic 40.0%
GAAATCATTCGGCTTAAGTG+GGG - chr8_2:49438861-49438880 None:intergenic 40.0%
TTGGGATACTGGTGATAGTA+GGG - chr8_2:49438937-49438956 None:intergenic 40.0%
! TATTGGTGGTAATGTGGGAA+GGG - chr8_2:49439009-49439028 None:intergenic 40.0%
!! GATGGTATTGGTGGTAATGT+GGG - chr8_2:49439014-49439033 None:intergenic 40.0%
!! TGATGGTATTGGTGGTAATG+TGG - chr8_2:49439015-49439034 None:intergenic 40.0%
! AGTGTTAGTGATGGTATTGG+TGG - chr8_2:49439023-49439042 None:intergenic 40.0%
AGTGATGGTAGGTATGATAG+GGG - chr8_2:49439263-49439282 None:intergenic 40.0%
GTAGTGATGGTAGGTATGAT+AGG - chr8_2:49439265-49439284 None:intergenic 40.0%
! GGAATTGATGGATGGTTGAA+TGG - chr8_2:49439451-49439470 None:intergenic 40.0%
GTTGTTGTGGAATTGATGGA+TGG - chr8_2:49439459-49439478 None:intergenic 40.0%
TAGTTGATTGCGCTTTCTCA+GGG - chr8_2:49439482-49439501 None:intergenic 40.0%
AAGACCATGATCCTTACCAT+TGG + chr8_2:49439901-49439920 Msa1252490:intron 40.0%
TCTTCCAATGGTAAGGATCA+TGG - chr8_2:49439908-49439927 None:intergenic 40.0%
CTTTCCAATGGTAAGGATCA+TGG - chr8_2:49439986-49440005 None:intergenic 40.0%
! AACATACGTGGTCTTAATCG+CGG + chr8_2:49440022-49440041 Msa1252490:intron 40.0%
! ATATCTAGGGTGCAATTGGT+AGG + chr8_2:49441019-49441038 Msa1252490:intron 40.0%
ATTGCGATATACAAGGGTCA+GGG + chr8_2:49441045-49441064 Msa1252490:intron 40.0%
CTAGGACATATCAAAGCATG+GGG - chr8_2:49441373-49441392 None:intergenic 40.0%
CGTCCCTGTTTATATGTGTA+TGG + chr8_2:49441461-49441480 Msa1252490:intron 40.0%
CACAACATAAGTGTTCTCAC+TGG - chr8_2:49441620-49441639 None:intergenic 40.0%
! ACAGAGTTCTGATTAACTGC+TGG - chr8_2:49441673-49441692 None:intergenic 40.0%
! TTATGCATTGGCAAAGAAGC+TGG + chr8_2:49441730-49441749 Msa1252490:CDS 40.0%
TTGCGCAAATGTCAGAAACT+TGG + chr8_2:49441756-49441775 Msa1252490:CDS 40.0%
AGTTCTTACCGATACGTCTA+TGG + chr8_2:49441834-49441853 Msa1252490:CDS 40.0%
! CGATACGTCTATGGAGTTTT+TGG + chr8_2:49441843-49441862 Msa1252490:CDS 40.0%
GACTTCAACCAGATCAATAG+TGG + chr8_2:49441931-49441950 Msa1252490:CDS 40.0%
! ATAGAGCACCACTATTGATC+TGG - chr8_2:49441942-49441961 None:intergenic 40.0%
TTGGTTGTGGTGGTTAGAGT+TGG - chr8_2:49438755-49438774 None:intergenic 45.0%
!! ATGGAGGTGTAGGGTTTTGA+GGG - chr8_2:49438790-49438809 None:intergenic 45.0%
! CATCAAAGCATGGAGGTGTA+GGG - chr8_2:49438799-49438818 None:intergenic 45.0%
! TCATCAAAGCATGGAGGTGT+AGG - chr8_2:49438800-49438819 None:intergenic 45.0%
TCGGCTTAAGTGGGGTAATT+GGG - chr8_2:49438853-49438872 None:intergenic 45.0%
TTCGGCTTAAGTGGGGTAAT+TGG - chr8_2:49438854-49438873 None:intergenic 45.0%
ATACTGGTGATAGTAGGGGT+GGG - chr8_2:49438932-49438951 None:intergenic 45.0%
TGGGATACTGGTGATAGTAG+GGG - chr8_2:49438936-49438955 None:intergenic 45.0%
GTTGGGATACTGGTGATAGT+AGG - chr8_2:49438938-49438957 None:intergenic 45.0%
! GTATTGGTGGTAATGTGGGA+AGG - chr8_2:49439010-49439029 None:intergenic 45.0%
! TAGTGAAGATGGTGGTAGTG+GGG - chr8_2:49439204-49439223 None:intergenic 45.0%
! GTAGTGAAGATGGTGGTAGT+GGG - chr8_2:49439205-49439224 None:intergenic 45.0%
GTCGTTGTAGTAGTGATGGT+AGG - chr8_2:49439274-49439293 None:intergenic 45.0%
! TGTTGGTTGCGATTTCACTG+CGG - chr8_2:49439417-49439436 None:intergenic 45.0%
AGGGGTTGTTGTGGAATTGA+TGG - chr8_2:49439463-49439482 None:intergenic 45.0%
AGTTGATTGCGCTTTCTCAG+GGG - chr8_2:49439481-49439500 None:intergenic 45.0%
GTAGTTGATTGCGCTTTCTC+AGG - chr8_2:49439483-49439502 None:intergenic 45.0%
CATGGCACACAAATGTCACT+TGG - chr8_2:49439686-49439705 None:intergenic 45.0%
TGACATTTGTGTGCCATGTG+AGG + chr8_2:49439688-49439707 Msa1252490:CDS 45.0%
GACATTTGTGTGCCATGTGA+GGG + chr8_2:49439689-49439708 Msa1252490:CDS 45.0%
ACGAGCCTTCAGAAGATTCA+TGG + chr8_2:49439715-49439734 Msa1252490:CDS 45.0%
CGAGCCTTCAGAAGATTCAT+GGG + chr8_2:49439716-49439735 Msa1252490:CDS 45.0%
CATGCCCATGAATCTTCTGA+AGG - chr8_2:49439723-49439742 None:intergenic 45.0%
CTTCAGAAGATTCATGGGCA+TGG + chr8_2:49439721-49439740 Msa1252490:CDS 45.0%
GGATGTGGAGACCCTTTAAT+GGG - chr8_2:49439765-49439784 None:intergenic 45.0%
TGGATGTGGAGACCCTTTAA+TGG - chr8_2:49439766-49439785 None:intergenic 45.0%
TTAAAGGGTCTCCACATCCA+AGG + chr8_2:49439766-49439785 Msa1252490:CDS 45.0%
AGGACCATGATCCTTACCAT+TGG + chr8_2:49439979-49439998 Msa1252490:intron 45.0%
! GTGTCGCTCATTGCAAAGAA+TGG - chr8_2:49440090-49440109 None:intergenic 45.0%
CCACACAGCGGAAAATTTCT+GGG - chr8_2:49440859-49440878 None:intergenic 45.0%
! CCAGAAATTTTCCGCTGTGT+GGG + chr8_2:49440857-49440876 Msa1252490:intron 45.0%
CATTGCGATATACAAGGGTC+AGG + chr8_2:49441044-49441063 Msa1252490:intron 45.0%
GACATATCAAAGCATGGGGA+AGG - chr8_2:49441369-49441388 None:intergenic 45.0%
!! CTTTGATGACTTGTTTCGGG+AGG - chr8_2:49441704-49441723 None:intergenic 45.0%
AACGGCAACATCGCCATATT+AGG - chr8_2:49441787-49441806 None:intergenic 45.0%
TTGCTCTCTGGCTGATCTAA+CGG - chr8_2:49441805-49441824 None:intergenic 45.0%
GGTGGTAGGAGTAGCAAAAG+TGG - chr8_2:49438714-49438733 None:intergenic 50.0%
GTTGTGGTGGTTAGAGTTGG+AGG - chr8_2:49438752-49438771 None:intergenic 50.0%
!!! TGGAGGTGTAGGGTTTTGAG+GGG - chr8_2:49438789-49438808 None:intergenic 50.0%
!! CATGGAGGTGTAGGGTTTTG+AGG - chr8_2:49438791-49438810 None:intergenic 50.0%
TGGGGCTGAGTTATTCTCCT+TGG - chr8_2:49438834-49438853 None:intergenic 50.0%
CGGCTTAAGTGGGGTAATTG+GGG - chr8_2:49438852-49438871 None:intergenic 50.0%
TACTGGTGATAGTAGGGGTG+GGG - chr8_2:49438931-49438950 None:intergenic 50.0%
GATACTGGTGATAGTAGGGG+TGG - chr8_2:49438933-49438952 None:intergenic 50.0%
GGTAGTGATGGTTGGGATAC+TGG - chr8_2:49438948-49438967 None:intergenic 50.0%
! TTGGGGTGGTAGTGATGGTT+GGG - chr8_2:49438955-49438974 None:intergenic 50.0%
TGAGCAGTGATGGTAGAGGT+GGG - chr8_2:49439178-49439197 None:intergenic 50.0%
!! AGTGAAGATGGTGGTAGTGG+GGG - chr8_2:49439203-49439222 None:intergenic 50.0%
! GGTAGTGAAGATGGTGGTAG+TGG - chr8_2:49439206-49439225 None:intergenic 50.0%
TGTAGGGGTAGTGAAGATGG+TGG - chr8_2:49439212-49439231 None:intergenic 50.0%
GGTTGTAGGGGTAGTGAAGA+TGG - chr8_2:49439215-49439234 None:intergenic 50.0%
! AGGTATGATAGGGGTAGTGG+TGG - chr8_2:49439254-49439273 None:intergenic 50.0%
GGTAGGTATGATAGGGGTAG+TGG - chr8_2:49439257-49439276 None:intergenic 50.0%
GTTGGTTGCGATTTCACTGC+GGG - chr8_2:49439416-49439435 None:intergenic 50.0%
CTCTAGGTGGGTGTGTGTTT+AGG - chr8_2:49439863-49439882 None:intergenic 50.0%
! CCCAGAAATTTTCCGCTGTG+TGG + chr8_2:49440856-49440875 Msa1252490:intron 50.0%
CCCACACAGCGGAAAATTTC+TGG - chr8_2:49440860-49440879 None:intergenic 50.0%
ATTATATGCGGCCCACACAG+CGG - chr8_2:49440871-49440890 None:intergenic 50.0%
TCTAAGTCAAGTGAGGGCCT+AGG + chr8_2:49441414-49441433 Msa1252490:intron 50.0%
GTGGTGGTTAGAGTTGGAGG+TGG - chr8_2:49438749-49438768 None:intergenic 55.0%
GATAGTAGGGGTGGGGATAG+TGG - chr8_2:49438924-49438943 None:intergenic 55.0%
! GTTGGGGTGGTAGTGATGGT+TGG - chr8_2:49438956-49438975 None:intergenic 55.0%
! AGTGGTTGGGGTGGTAGTGA+TGG - chr8_2:49438960-49438979 None:intergenic 55.0%
AGGGGGTAGGAGTAGTGGTT+GGG - chr8_2:49438973-49438992 None:intergenic 55.0%
! AAGGGATGGTGGTGCTTGAG+GGG - chr8_2:49438991-49439010 None:intergenic 55.0%
! GAAGGGATGGTGGTGCTTGA+GGG - chr8_2:49438992-49439011 None:intergenic 55.0%
GGTAATGTGGGAAGGGATGG+TGG - chr8_2:49439002-49439021 None:intergenic 55.0%
! GGTGGTAATGTGGGAAGGGA+TGG - chr8_2:49439005-49439024 None:intergenic 55.0%
! AGGTGGGGATAGTGGTGTTG+GGG - chr8_2:49439162-49439181 None:intergenic 55.0%
! GAGGTGGGGATAGTGGTGTT+GGG - chr8_2:49439163-49439182 None:intergenic 55.0%
! AGAGGTGGGGATAGTGGTGT+TGG - chr8_2:49439164-49439183 None:intergenic 55.0%
GATGGTAGAGGTGGGGATAG+TGG - chr8_2:49439170-49439189 None:intergenic 55.0%
GAGCAGTGATGGTAGAGGTG+GGG - chr8_2:49439177-49439196 None:intergenic 55.0%
GTGAGCAGTGATGGTAGAGG+TGG - chr8_2:49439179-49439198 None:intergenic 55.0%
GGTGTGAGCAGTGATGGTAG+AGG - chr8_2:49439182-49439201 None:intergenic 55.0%
GGTGGCGAATGTGGTTGTAG+GGG - chr8_2:49439227-49439246 None:intergenic 55.0%
GGGTGGCGAATGTGGTTGTA+GGG - chr8_2:49439228-49439247 None:intergenic 55.0%
AGGGTGGCGAATGTGGTTGT+AGG - chr8_2:49439229-49439248 None:intergenic 55.0%
! GATAGGGGTAGTGGTGGTGA+GGG - chr8_2:49439248-49439267 None:intergenic 55.0%
! TGATAGGGGTAGTGGTGGTG+AGG - chr8_2:49439249-49439268 None:intergenic 55.0%
TGCGATTTCACTGCGGGCAT+GGG - chr8_2:49439410-49439429 None:intergenic 55.0%
TTGCGATTTCACTGCGGGCA+TGG - chr8_2:49439411-49439430 None:intergenic 55.0%
CGCTTTCTCAGGGGTTGTTG+TGG - chr8_2:49439472-49439491 None:intergenic 55.0%
AAGGCTCGTCTTCCCTCACA+TGG - chr8_2:49439704-49439723 None:intergenic 55.0%
AGATCAGCCAGAGAGCAACG+AGG + chr8_2:49441807-49441826 Msa1252490:CDS 55.0%
ACTTGAGCCTCGTTGCTCTC+TGG - chr8_2:49441817-49441836 None:intergenic 55.0%
! GGGGTGGGGATAGTGGTTGT+TGG - chr8_2:49438917-49438936 None:intergenic 60.0%
GGTAGGAGTAGTGGTTGGGG+TGG - chr8_2:49438969-49438988 None:intergenic 60.0%
GGGGGTAGGAGTAGTGGTTG+GGG - chr8_2:49438972-49438991 None:intergenic 60.0%
GAGGGGGTAGGAGTAGTGGT+TGG - chr8_2:49438974-49438993 None:intergenic 60.0%
GCTTGAGGGGGTAGGAGTAG+TGG - chr8_2:49438978-49438997 None:intergenic 60.0%
! ATGGTGGTGCTTGAGGGGGT+AGG - chr8_2:49438986-49439005 None:intergenic 60.0%
! AGGGATGGTGGTGCTTGAGG+GGG - chr8_2:49438990-49439009 None:intergenic 60.0%
! GGAAGGGATGGTGGTGCTTG+AGG - chr8_2:49438993-49439012 None:intergenic 60.0%
! AGTGGGGGTGTGAGCAGTGA+TGG - chr8_2:49439188-49439207 None:intergenic 60.0%
GCGATTTCACTGCGGGCATG+GGG - chr8_2:49439409-49439428 None:intergenic 60.0%
! ACCCACGTTGGTGGGTGTGT+GGG - chr8_2:49441440-49441459 None:intergenic 60.0%
AAACAGGGACGGACCCACGT+TGG - chr8_2:49441452-49441471 None:intergenic 60.0%
GGTGGTGAGGGTGGCGAATG+TGG - chr8_2:49439236-49439255 None:intergenic 65.0%
! AGGGGTAGTGGTGGTGAGGG+TGG - chr8_2:49439245-49439264 None:intergenic 65.0%
! GTTGGTGGGTGTGTGGGCCT+AGG - chr8_2:49441434-49441453 None:intergenic 65.0%
GCCCACACACCCACCAACGT+GGG + chr8_2:49441436-49441455 Msa1252490:intron 65.0%
! GACCCACGTTGGTGGGTGTG+TGG - chr8_2:49441441-49441460 None:intergenic 65.0%
AGGGACGGACCCACGTTGGT+GGG - chr8_2:49441448-49441467 None:intergenic 65.0%
GGCCCACACACCCACCAACG+TGG + chr8_2:49441435-49441454 Msa1252490:intron 70.0%
CAGGGACGGACCCACGTTGG+TGG - chr8_2:49441449-49441468 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 49438659 49441969 49438659 ID=Msa1252490;Name=Msa1252490
chr8_2 mRNA 49438659 49441969 49438659 ID=Msa1252490-mRNA-1;Parent=Msa1252490;Name=Msa1252490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|3|0|485
chr8_2 exon 49438659 49439581 49438659 ID=Msa1252490-mRNA-1:exon:14942;Parent=Msa1252490-mRNA-1
chr8_2 exon 49439671 49439787 49439671 ID=Msa1252490-mRNA-1:exon:14943;Parent=Msa1252490-mRNA-1
chr8_2 exon 49441552 49441969 49441552 ID=Msa1252490-mRNA-1:exon:14944;Parent=Msa1252490-mRNA-1
chr8_2 CDS 49438659 49439581 49438659 ID=Msa1252490-mRNA-1:cds;Parent=Msa1252490-mRNA-1
chr8_2 CDS 49439671 49439787 49439671 ID=Msa1252490-mRNA-1:cds;Parent=Msa1252490-mRNA-1
chr8_2 CDS 49441552 49441969 49441552 ID=Msa1252490-mRNA-1:cds;Parent=Msa1252490-mRNA-1
Gene Sequence

>Msa1252490

ATGGACAATGATGATTGGGATCTCTTTTCTATTGTTCGTAGGTGCAAAGCTACCACTTTTGCTACTCCTACCACCAATTTTGAAACTCCACCTCCAACTCTAACCACCACAACCAATAACATCATATCCCCTCAAAACCCTACACCTCCATGCTTTGATGATTTTAGTTTTACCAAGGAGAATAACTCAGCCCCAATTACCCCACTTAAGCCGAATGATTTCATAGAGCTTGACAAATTGATAATCAGTTTCAATCCAACAACCACTATCCCCACCCCTACTATCACCAGTATCCCAACCATCACTACCACCCCAACCACTACTCCTACCCCCTCAAGCACCACCATCCCTTCCCACATTACCACCAATACCATCACTAACACTAGTTTTCATGATTCCAATCAAAATTCCACCTTTTCTAATTTCCCAACACAAATCGAACAACAACAACTGCAACTAAATGAGTTTACTGAGCTAGAAAAAATGGTACTTAAATTCTACCCCAACACCACTATCCCCACCTCTACCATCACTGCTCACACCCCCACTACCACCATCTTCACTACCCCTACAACCACATTCGCCACCCTCACCACCACTACCCCTATCATACCTACCATCACTACTACAACGACTAATACTAGTGTTTGTAAAACAAATCAATATCTTACAATTTCTGACTATCAAATACTCGTCGAACAACAACAGAATCAACCAAACCGTAACAATGAAGTGTATGTTTCTGAACCCCATGCCCGCAGTGAAATCGCAACCAACAATTTTGATTGTCCATTCAACCATCCATCAATTCCACAACAACCCCTGAGAAAGCGCAATCAACTACCATTTCTACTACCTCGAACAAATTCTAGATTTTTACCAAATACACATCCGAAAAAACCAAAATTTAAATCAAGAAAAAGAAAAAATAATAACCAAGTGACATTTGTGTGCCATGTGAGGGAAGACGAGCCTTCAGAAGATTCATGGGCATGGAAAAAATATGGAGAAAAACCCATTAAAGGGTCTCCACATCCAAGGAGCTACTACAAATGTAGAAGTTTCTGGGATTGTTCGGCAAAAAAACTAGTAGAGAAAAGCAAAACCAGTGAGAACACTTATGTTGTGACATATAAAGGAGAACACAATCATGAAAAACCAGCAGTTAATCAGAACTCTGTTGTTGGGACCTCCCGAAACAAGTCATCAAAGACTAGATTATGCATTGGCAAAGAAGCTGGATCTTGCGCAAATGTCAGAAACTTGGAATCGCCTAATATGGCGATGTTGCCGTTAGATCAGCCAGAGAGCAACGAGGCTCAAGTTCTTACCGATACGTCTATGGAGTTTTTGGGAAGCTCTGATGACGATGTCATTTTGATACCAAACATGACACCTATGTTAGAGGATCTCTTGATGGACTTCAACCAGATCAATAGTGGTGCTCTATTCCCTTAG
Protein sequence

>Msa1252490

MDNDDWDLFSIVRRCKATTFATPTTNFETPPPTLTTTTNNIISPQNPTPPCFDDFSFTKENNSAPITPLKPNDFIELDKLIISFNPTTTIPTPTITSIPTITTTPTTTPTPSSTTIPSHITTNTITNTSFHDSNQNSTFSNFPTQIEQQQLQLNEFTELEKMVLKFYPNTTIPTSTITAHTPTTTIFTTPTTTFATLTTTTPIIPTITTTTTNTSVCKTNQYLTISDYQILVEQQQNQPNRNNEVYVSEPHARSEIATNNFDCPFNHPSIPQQPLRKRNQLPFLLPRTNSRFLPNTHPKKPKFKSRKRKNNNQVTFVCHVREDEPSEDSWAWKKYGEKPIKGSPHPRSYYKCRSFWDCSAKKLVEKSKTSENTYVVTYKGEHNHEKPAVNQNSVVGTSRNKSSKTRLCIGKEAGSCANVRNLESPNMAMLPLDQPESNEAQVLTDTSMEFLGSSDDDVILIPNMTPMLEDLLMDFNQINSGALFP*