AlfalfaGEDB | Msa1253140

M. sativa cultivar zhongmu-4 / Msa1253140

KEGG: K00924|K03083
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009933 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0032502 GO:0036211 GO:0042538 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0047484 GO:0048507 GO:0048518 GO:0048532 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050896 GO:0065007 GO:0071704 GO:0080134 GO:0140096 GO:1901000 GO:1901002 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253140	XP_020230291.1	90.541	74	7	0	2	75	157	230	5.15e-44	151

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253140	sp\|P51138\|MSK2_MEDSA	87.838	74	9	0	2	75	238	311	5.80e-43	146

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253140	A0A392NZV8	90.541	74	7	0	2	75	13	86	4.25e-42	143

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253140	MtrunA17_Chr1g0194201	87.838	74	9	0	2	75	239	312	4.64e-44	146
Msa1253140	MtrunA17_Chr7g0270371	90.541	74	7	0	2	75	239	312	7.48e-44	145
Msa1253140	MtrunA17_Chr7g0246591	85.135	74	11	0	2	75	305	378	1.16e-43	146
Msa1253140	MtrunA17_Chr1g0193081	86.486	74	10	0	2	75	239	312	6.48e-42	140
Msa1253140	MtrunA17_Chr2g0277011	86.486	74	10	0	2	75	254	327	1.66e-41	139
Msa1253140	MtrunA17_Chr8g0350311	81.081	74	14	0	2	75	303	376	1.51e-40	137
Msa1253140	MtrunA17_Chr2g0320011	85.135	74	11	0	2	75	252	325	2.17e-40	136
Msa1253140	MtrunA17_Chr2g0284091	79.730	74	15	0	2	75	204	277	6.49e-40	134
Msa1253140	MtrunA17_Chr7g0273601	50.000	72	35	1	3	73	272	343	1.08e-18	78.2
Msa1253140	MtrunA17_Chr5g0396361	49.153	59	30	0	3	61	305	363	1.90e-18	77.4

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253140	AT3G05840.2	87.838	74	9	2	75	237	310	1.42e-43	145
Msa1253140	AT3G05840.1	87.838	74	9	2	75	237	310	1.42e-43	145
Msa1253140	AT5G14640.1	89.189	74	8	2	75	238	311	6.16e-43	143
Msa1253140	AT5G14640.2	89.189	74	8	2	75	238	311	6.16e-43	143
Msa1253140	AT1G57870.1	86.486	74	10	2	75	246	319	9.09e-43	143
Msa1253140	AT1G57870.4	86.486	74	10	2	75	246	319	9.09e-43	143
Msa1253140	AT1G57870.2	86.486	74	10	2	75	246	319	9.09e-43	143
Msa1253140	AT1G09840.3	85.135	74	11	2	75	247	320	1.23e-42	142
Msa1253140	AT1G09840.7	85.135	74	11	2	75	247	320	1.23e-42	142
Msa1253140	AT1G09840.10	85.135	74	11	2	75	247	320	1.23e-42	142

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Find 25 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTTTGTAGGTTAATCA+AGG	0.473313	8_2:-50409682	Msa1253140:three_prime_UTR
AAGTCGTATCGAAGAATATA+TGG	0.350780	8_2:-50409593	Msa1253140:three_prime_UTR
ACACCACAGTCATTGACATC+TGG	0.524089	8_2:-50410115	Msa1253140:CDS
AGAGCTCCTGAGCCTATATT+CGG	0.474591	8_2:-50410149	Msa1253140:CDS
ATTCAATCATAGCTCAGTTA+TGG	0.423401	8_2:+50409537	None:intergenic
CACTTGATTTCTTCCCTTGT+TGG	0.201375	8_2:+50409749	None:intergenic
CCAACTTGTGGAAATTATCA+AGG	0.511188	8_2:-50409937	Msa1253140:intron
CCACTCTCACCAGGAAACAG+TGG	0.653830	8_2:+50409966	None:intergenic
CCACTGTTTCCTGGTGAGAG+TGG	0.506242	8_2:-50409966	Msa1253140:CDS
CCTTGATAATTTCCACAAGT+TGG	0.452229	8_2:+50409937	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTGTGCATGTCACAAAT+CGG	+	chr8_2:50409558-50409577	None:intergenic	30.0%
TGTTATGATTTCTACCAGAA+AGG	-	chr8_2:50409611-50409630	Msa1253140:three_prime_UTR	30.0%
ATTTCACTTTCCTAACAGAA+TGG	-	chr8_2:50409758-50409777	Msa1253140:CDS	30.0%
ATTACTATCTCCATTCTGTT+AGG	+	chr8_2:50409771-50409790	None:intergenic	30.0%
AAACTAACGAAAGGATTTCA+AGG	+	chr8_2:50409891-50409910	None:intergenic	30.0%
GTTATTGCAAAACTAACGAA+AGG	+	chr8_2:50409900-50409919	None:intergenic	30.0%
GCATGAATCCTAATTATACT+AGG	-	chr8_2:50410429-50410448	Msa1253140:intron	30.0%
TAAACTTGGGAAATCTTTGT+AGG	-	chr8_2:50410464-50410483	Msa1253140:intron	30.0%
AAGTCGTATCGAAGAATATA+TGG	-	chr8_2:50410563-50410582	Msa1253140:intron	30.0%
ATTCAATCATAGCTCAGTTA+TGG	+	chr8_2:50410622-50410641	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	50409452	50410726	50409452	ID=Msa1253140;Name=Msa1253140
chr8_2	mRNA	50409452	50410726	50409452	ID=Msa1253140-mRNA-1;Parent=Msa1253140;Name=Msa1253140-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|0.66\|0.5\|0.75\|1\|1\|4\|260\|76
chr8_2	exon	50409452	50409783	50409452	ID=Msa1253140-mRNA-1:exon:15367;Parent=Msa1253140-mRNA-1
chr8_2	exon	50409938	50409988	50409938	ID=Msa1253140-mRNA-1:exon:15366;Parent=Msa1253140-mRNA-1
chr8_2	exon	50410076	50410178	50410076	ID=Msa1253140-mRNA-1:exon:15365;Parent=Msa1253140-mRNA-1
chr8_2	exon	50410722	50410726	50410722	ID=Msa1253140-mRNA-1:exon:15364;Parent=Msa1253140-mRNA-1
chr8_2	CDS	50410722	50410726	50410722	ID=Msa1253140-mRNA-1:cds;Parent=Msa1253140-mRNA-1
chr8_2	CDS	50410076	50410178	50410076	ID=Msa1253140-mRNA-1:cds;Parent=Msa1253140-mRNA-1
chr8_2	CDS	50409938	50409988	50409938	ID=Msa1253140-mRNA-1:cds;Parent=Msa1253140-mRNA-1
chr8_2	CDS	50409712	50409783	50409712	ID=Msa1253140-mRNA-1:cds;Parent=Msa1253140-mRNA-1
chr8_2	three_prime_UTR	50409452	50409711	50409452	ID=Msa1253140-mRNA-1:three_prime_utr;Parent=Msa1253140-mRNA-1

Gene Sequence	>Msa1253140 ATGAAGTACTATAGAGCTCCTGAGCCTATATTCGGTGCAACTGAGTACACCACAGTCATTGACATCTGGTCAGCTGGTTGCGTACTTGGTGAACTTTTGCTTGGCCAGCCACTGTTTCCTGGTGAGAGTGGAGTAGACCAACTTGTGGAAATTATCAAGGTTTTAGGTACTCCAACAAGGGAAGAAATCAAGTGCATGAATCCTAATTATACTAGGTATAATTATATATAA
Protein sequence	>Msa1253140 MKYYRAPEPIFGATEYTTVIDIWSAGCVLGELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTRYNYI*