AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1253160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1253160 MtrunA17_Chr7g0228891 88.764 89 10 0 1 89 1 89 1.37e-52 165
Msa1253160 MtrunA17_Chr3g0110351 74.157 89 23 0 1 89 1 89 6.03e-42 139
Msa1253160 MtrunA17_Chr5g0434571 74.157 89 23 0 1 89 37 125 1.35e-41 139
Msa1253160 MtrunA17_Chr4g0057031 70.787 89 26 0 1 89 1 89 3.69e-40 133
Msa1253160 MtrunA17_Chr8g0349261 67.368 95 21 3 1 89 1 91 9.41e-38 127
Msa1253160 MtrunA17_Chr6g0453471 65.169 89 31 0 1 89 1 89 2.47e-37 128
Msa1253160 MtrunA17_Chr7g0261281 64.045 89 32 0 1 89 1 89 3.86e-37 125
Msa1253160 MtrunA17_Chr3g0115821 61.798 89 34 0 1 89 1 89 1.28e-36 125
Msa1253160 MtrunA17_Chr7g0271241 64.045 89 32 0 1 89 1 89 1.71e-36 126
Msa1253160 MtrunA17_Chr4g0039971 64.045 89 32 0 1 89 2 90 3.90e-36 124
Msa1253160 MtrunA17_Chr1g0193371 62.921 89 33 0 1 89 1 89 4.09e-36 125
Msa1253160 MtrunA17_Chr1g0187761 62.921 89 33 0 1 89 1 89 4.22e-36 122
Msa1253160 MtrunA17_Chr7g0217741 64.045 89 32 0 1 89 1 89 5.00e-36 123
Msa1253160 MtrunA17_Chr4g0042671 64.045 89 32 0 1 89 1 89 1.37e-35 124
Msa1253160 MtrunA17_Chr1g0203411 62.921 89 33 0 1 89 1 89 5.66e-35 120
Msa1253160 MtrunA17_Chr4g0042611 62.500 88 33 0 1 88 1 88 8.56e-35 114
Msa1253160 MtrunA17_Chr2g0323411 64.045 89 32 0 1 89 1 89 1.14e-34 120
Msa1253160 MtrunA17_Chr1g0167691 60.674 89 35 0 1 89 1 89 1.17e-34 118
Msa1253160 MtrunA17_Chr2g0330041 61.798 89 34 0 1 89 1 89 3.31e-34 120
Msa1253160 MtrunA17_Chr2g0294811 62.500 88 33 0 1 88 1 88 4.43e-34 113
Msa1253160 MtrunA17_Chr3g0131121 60.674 89 35 0 1 89 1 89 5.40e-34 118
Msa1253160 MtrunA17_Chr4g0048511 62.222 90 33 1 1 89 1 90 5.76e-34 119
Msa1253160 MtrunA17_Chr8g0385321 56.180 89 39 0 1 89 1 89 1.11e-33 117
Msa1253160 MtrunA17_Chr2g0281071 61.111 90 34 1 1 89 1 90 1.11e-33 118
Msa1253160 MtrunA17_Chr4g0037141 58.427 89 37 0 1 89 1 89 1.43e-33 116
Msa1253160 MtrunA17_Chr1g0194951 62.921 89 33 0 1 89 1 89 3.87e-33 117
Msa1253160 MtrunA17_Chr8g0346561 58.427 89 37 0 1 89 1 89 4.55e-33 115
Msa1253160 MtrunA17_Chr7g0275911 58.427 89 37 0 1 89 1 89 6.80e-33 114
Msa1253160 MtrunA17_Chr4g0058631 61.798 89 34 0 1 89 1 89 6.91e-33 116
Msa1253160 MtrunA17_Chr3g0121241 61.176 85 33 0 5 89 35 119 7.07e-33 115
Msa1253160 MtrunA17_Chr7g0274581 55.056 89 40 0 1 89 1 89 9.29e-33 112
Msa1253160 MtrunA17_Chr7g0270891 60.674 89 35 0 1 89 1 89 1.98e-32 115
Msa1253160 MtrunA17_Chr1g0167661 60.674 89 35 0 1 89 1 89 2.26e-32 112
Msa1253160 MtrunA17_Chr4g0068061 59.551 89 36 0 1 89 1 89 3.39e-32 114
Msa1253160 MtrunA17_Chr5g0410961 58.427 89 37 0 1 89 1 89 3.52e-32 114
Msa1253160 MtrunA17_Chr8g0342541 58.427 89 37 0 1 89 1 89 4.47e-32 114
Msa1253160 MtrunA17_Chr3g0117091 61.798 89 34 0 1 89 1 89 5.85e-32 114
Msa1253160 MtrunA17_Chr4g0060721 56.180 89 39 0 1 89 2 90 5.88e-32 113
Msa1253160 MtrunA17_Chr8g0342511 58.621 87 36 0 1 87 1 87 6.23e-32 107
Msa1253160 MtrunA17_Chr6g0453211 58.427 89 37 0 1 89 1 89 1.08e-31 112
Msa1253160 MtrunA17_Chr8g0342531 58.427 89 37 0 1 89 1 89 1.16e-31 108
Msa1253160 MtrunA17_Chr5g0435101 57.471 87 37 0 3 89 2 88 1.40e-31 109
Msa1253160 MtrunA17_Chr4g0070641 59.551 89 36 0 1 89 1 89 1.60e-31 108
Msa1253160 MtrunA17_Chr3g0103281 57.303 89 38 0 1 89 1 89 1.63e-31 112
Msa1253160 MtrunA17_Chr1g0193801 59.551 89 36 0 1 89 1 89 1.72e-31 113
Msa1253160 MtrunA17_Chr2g0323351 56.989 93 32 2 1 89 1 89 2.98e-31 109
Msa1253160 MtrunA17_Chr2g0311221 60.227 88 35 0 2 89 4 91 3.29e-31 111
Msa1253160 MtrunA17_Chr8g0342521 57.303 89 38 0 1 89 1 89 3.31e-31 111
Msa1253160 MtrunA17_Chr4g0022901 53.191 94 35 3 1 89 1 90 4.41e-31 109
Msa1253160 MtrunA17_Chr7g0217221 58.427 89 37 0 1 89 1 89 6.35e-31 109
Msa1253160 MtrunA17_Chr8g0342461 58.889 90 36 1 1 89 1 90 7.47e-31 105
Msa1253160 MtrunA17_Chr4g0022931 53.191 94 35 3 1 89 1 90 7.78e-31 109
Msa1253160 MtrunA17_Chr8g0342471 58.889 90 36 1 1 89 1 90 8.15e-31 105
Msa1253160 MtrunA17_Chr4g0022911 51.064 94 37 3 1 89 1 90 9.25e-31 109
Msa1253160 MtrunA17_Chr3g0092401 56.180 89 39 0 1 89 2 90 1.30e-30 109
Msa1253160 MtrunA17_Chr7g0254411 57.303 89 38 0 1 89 1 89 1.52e-30 109
Msa1253160 MtrunA17_Chr4g0070611 59.551 89 36 0 1 89 1 89 1.93e-30 108
Msa1253160 MtrunA17_Chr8g0342481 57.778 90 37 1 1 89 1 90 2.54e-30 110
Msa1253160 MtrunA17_Chr6g0452271 55.914 93 33 2 1 89 1 89 5.56e-30 108
Msa1253160 MtrunA17_Chr2g0323321 55.914 93 32 3 1 89 1 88 1.03e-29 102
Msa1253160 MtrunA17_Chr5g0397231 53.933 89 41 0 1 89 1 89 1.17e-29 107
Msa1253160 MtrunA17_Chr8g0387421 53.933 89 41 0 1 89 1 89 2.55e-29 106
Msa1253160 MtrunA17_Chr7g0269831 60.674 89 35 0 1 89 1 89 2.77e-29 107
Msa1253160 MtrunA17_Chr1g0151941 52.128 94 36 2 1 89 1 90 3.14e-29 104
Msa1253160 MtrunA17_Chr4g0032401 60.256 78 31 0 12 89 17 94 3.44e-29 106
Msa1253160 MtrunA17_Chr1g0211161 60.920 87 34 0 3 89 4 90 4.00e-29 103
Msa1253160 MtrunA17_Chr5g0400821 52.128 94 36 2 1 89 1 90 4.64e-29 105
Msa1253160 MtrunA17_Chr1g0152011 52.128 94 36 2 1 89 1 90 5.12e-29 105
Msa1253160 MtrunA17_Chr4g0044421 56.818 88 37 1 3 89 2 89 7.76e-29 102
Msa1253160 MtrunA17_Chr4g0028311 52.128 94 36 2 1 89 1 90 8.59e-29 105
Msa1253160 MtrunA17_Chr3g0135311 51.064 94 37 2 1 89 1 90 1.28e-28 105
Msa1253160 MtrunA17_Chr4g0054391 53.191 94 35 2 1 89 1 90 1.29e-28 103
Msa1253160 MtrunA17_Chr2g0327351 52.128 94 36 2 1 89 1 90 1.70e-28 104
Msa1253160 MtrunA17_Chr2g0309911 57.303 89 37 1 1 89 1 88 1.88e-28 103
Msa1253160 MtrunA17_Chr5g0395221 54.545 88 40 0 1 88 1 88 2.72e-28 97.8
Msa1253160 MtrunA17_Chr7g0264641 51.064 94 37 2 1 89 1 90 3.31e-28 102
Msa1253160 MtrunA17_Chr4g0022891 52.809 89 42 0 1 89 1 89 4.59e-28 101
Msa1253160 MtrunA17_Chr5g0395281 56.180 89 39 0 1 89 1 89 5.13e-28 102
Msa1253160 MtrunA17_Chr2g0328771 57.692 78 33 0 12 89 20 97 2.91e-27 100
Msa1253160 MtrunA17_Chr4g0022881 49.438 89 45 0 1 89 1 89 1.73e-26 97.1
Msa1253160 MtrunA17_Chr4g0072751 50.000 94 38 2 1 89 1 90 2.43e-26 99.0
Msa1253160 MtrunA17_Chr6g0487491 52.688 93 35 2 1 88 1 89 5.27e-26 96.7
Msa1253160 MtrunA17_Chr5g0417821 53.750 80 37 0 10 89 5 84 7.38e-26 96.7
Msa1253160 MtrunA17_Chr8g0354661 56.250 80 35 0 10 89 35 114 1.09e-25 98.6
Msa1253160 MtrunA17_Chr1g0203441 54.444 90 40 1 1 89 1 90 1.49e-25 95.5
Msa1253160 MtrunA17_Chr6g0450101 48.352 91 45 1 1 89 1 91 3.14e-25 93.2
Msa1253160 MtrunA17_Chr7g0228231 53.846 78 36 0 12 89 14 91 9.22e-25 92.0
Msa1253160 MtrunA17_Chr6g0470971 54.321 81 37 0 9 89 11 91 1.88e-24 92.8
Msa1253160 MtrunA17_Chr3g0099141 48.837 86 36 2 8 89 36 117 2.49e-24 89.4
Msa1253160 MtrunA17_Chr3g0088461 53.659 82 38 0 8 89 18 99 6.45e-24 92.4
Msa1253160 MtrunA17_Chr3g0099131 50.000 82 33 2 12 89 41 118 1.47e-23 92.8
Msa1253160 MtrunA17_Chr1g0209661 50.000 78 39 0 12 89 27 104 2.51e-23 90.5
Msa1253160 MtrunA17_Chr1g0186061 53.247 77 36 0 13 89 21 97 3.46e-23 89.0
Msa1253160 MtrunA17_Chr3g0080861 52.500 80 38 0 10 89 35 114 3.53e-23 91.7
Msa1253160 MtrunA17_Chr1g0168661 53.247 77 36 0 13 89 22 98 8.11e-23 88.2
Msa1253160 MtrunA17_Chr2g0293911 50.000 82 41 0 8 89 20 101 2.34e-22 88.2
Msa1253160 MtrunA17_Chr1g0194291 48.780 82 42 0 8 89 17 98 3.71e-22 86.7
Msa1253160 MtrunA17_Chr1g0194301 47.561 82 43 0 8 89 29 110 4.66e-22 86.7
Msa1253160 MtrunA17_Chr1g0154031 51.220 82 32 2 12 89 76 153 4.70e-22 88.2
Msa1253160 MtrunA17_Chr4g0055981 52.941 85 32 2 9 89 52 132 1.92e-21 84.0
Msa1253160 MtrunA17_Chr2g0322521 52.941 85 32 2 9 89 26 106 2.31e-21 85.9
Msa1253160 MtrunA17_Chr6g0453751 46.591 88 31 2 11 86 19 102 6.36e-21 80.1
Msa1253160 MtrunA17_Chr4g0068921 48.780 82 42 0 8 89 20 101 7.91e-21 81.6
Msa1253160 MtrunA17_Chr7g0240821 55.405 74 33 0 16 89 5 78 2.65e-20 80.9
Msa1253160 MtrunA17_Chr1g0192411 42.683 82 47 0 8 89 8 89 3.44e-20 82.8
Msa1253160 MtrunA17_Chr5g0425591 42.683 82 47 0 5 86 2 83 5.42e-20 76.6
Msa1253160 MtrunA17_Chr2g0327821 47.436 78 41 0 12 89 6 83 8.08e-20 79.7
Msa1253160 MtrunA17_Chr7g0247131 74.468 47 12 0 43 89 9 55 4.09e-19 78.6
Msa1253160 MtrunA17_Chr1g0180391 44.186 86 48 0 4 89 11 96 4.94e-19 77.8
Msa1253160 MtrunA17_Chr5g0434831 50.633 79 39 0 11 89 6 84 7.37e-19 76.6
Msa1253160 MtrunA17_Chr4g0030881 45.238 84 39 1 13 89 8 91 1.04e-18 77.4
Msa1253160 MtrunA17_Chr8g0343831 47.436 78 41 0 12 89 8 85 1.57e-18 76.6
Msa1253160 MtrunA17_Chr7g0220341 47.619 84 42 1 8 89 21 104 1.91e-18 77.0
Msa1253160 MtrunA17_Chr5g0434841 49.367 79 40 0 11 89 19 97 2.26e-18 77.0
Msa1253160 MtrunA17_Chr5g0434931 49.367 79 40 0 11 89 34 112 2.64e-18 76.6
Msa1253160 MtrunA17_Chr8g0360111 49.367 79 40 0 11 89 24 102 2.92e-18 76.3
Msa1253160 MtrunA17_Chr5g0434911 46.988 83 44 0 7 89 16 98 8.03e-18 75.1
Msa1253160 MtrunA17_Chr5g0440781 41.975 81 39 2 10 86 10 86 2.26e-17 72.4
Msa1253160 MtrunA17_Chr7g0228991 49.367 79 40 0 11 89 28 106 2.59e-17 73.9
Msa1253160 MtrunA17_Chr5g0441141 43.590 78 36 2 13 86 13 86 2.75e-17 73.9
Msa1253160 MtrunA17_Chr5g0440761 41.975 81 39 2 10 86 10 86 1.06e-16 72.0
Msa1253160 MtrunA17_Chr5g0401731 40.000 80 39 2 14 89 15 89 1.77e-15 69.3
Msa1253160 MtrunA17_Chr3g0117511 37.647 85 44 2 9 89 178 257 3.34e-15 68.9
Msa1253160 MtrunA17_Chr5g0441131 38.372 86 45 2 5 86 2 83 7.34e-15 65.9
Msa1253160 MtrunA17_Chr8g0334881 40.909 88 41 4 8 89 6 88 9.53e-15 67.4
Msa1253160 MtrunA17_Chr6g0454921 40.000 80 39 2 14 89 15 89 2.42e-14 65.1
Msa1253160 MtrunA17_Chr5g0429661 42.683 82 38 3 12 89 120 196 2.74e-14 66.2
Msa1253160 MtrunA17_Chr4g0013361 42.857 84 37 4 12 89 10 88 5.81e-14 65.1
Msa1253160 MtrunA17_Chr4g0053431 37.500 80 41 2 14 89 5 79 3.71e-13 62.8
Msa1253160 MtrunA17_Chr5g0417621 42.500 80 37 3 14 89 134 208 6.12e-13 62.4
Msa1253160 MtrunA17_Chr4g0042501 41.250 80 38 3 14 89 105 179 2.29e-12 60.8
Msa1253160 MtrunA17_Chr4g0058991 35.000 80 43 2 14 89 209 283 2.99e-12 60.5
Msa1253160 MtrunA17_Chr6g0460671 37.500 80 41 2 14 89 99 173 7.58e-12 59.3
Msa1253160 MtrunA17_Chr6g0480581 43.750 80 36 3 14 89 4 78 7.94e-12 58.5
Msa1253160 MtrunA17_Chr5g0434901 61.905 42 16 0 48 89 9 50 8.82e-12 56.6
Msa1253160 MtrunA17_Chr7g0229131 57.447 47 20 0 43 89 3 49 9.65e-12 57.4
Msa1253160 MtrunA17_Chr4g0008551 42.500 80 37 4 14 89 38 112 1.59e-11 58.5
Msa1253160 MtrunA17_Chr7g0247141 51.020 49 23 1 1 48 1 49 1.89e-11 54.3
Msa1253160 MtrunA17_Chr7g0238631 35.366 82 44 2 12 89 111 187 1.94e-11 58.2
Msa1253160 MtrunA17_Chr7g0229101 57.447 47 20 0 43 89 3 49 2.39e-11 57.0
Msa1253160 MtrunA17_Chr3g0139671 32.927 82 46 2 12 89 83 159 2.94e-11 57.8
Msa1253160 MtrunA17_Chr6g0460661 38.750 80 40 3 14 89 16 90 3.28e-11 55.8
Msa1253160 MtrunA17_Chr3g0133601 36.047 86 46 2 8 89 31 111 5.05e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1253160 AT5G14340.1 77.528 89 20 0 1 89 13 101 1.34e-46 150
Msa1253160 AT5G16600.1 74.157 89 23 0 1 89 1 89 4.88e-42 139
Msa1253160 AT3G23250.1 68.539 89 28 0 1 89 1 89 2.74e-41 137
Msa1253160 AT1G66230.1 70.787 89 26 0 1 89 1 89 1.53e-40 134
Msa1253160 AT3G61250.1 67.416 89 29 0 1 89 1 89 1.61e-39 132
Msa1253160 AT4G22680.1 67.416 89 29 0 1 89 1 89 3.77e-38 128
Msa1253160 AT5G15310.2 64.045 89 32 0 1 89 1 89 2.66e-37 127
Msa1253160 AT4G12350.1 67.416 89 29 0 1 89 1 89 2.85e-37 126
Msa1253160 AT5G15310.1 64.045 89 32 0 1 89 1 89 2.96e-37 127
Msa1253160 AT1G06180.1 65.169 89 31 0 1 89 1 89 3.30e-37 125
Msa1253160 AT3G01140.2 64.045 89 32 0 1 89 1 89 7.09e-37 127
Msa1253160 AT4G28110.1 60.674 89 35 0 1 89 1 89 2.46e-36 124
Msa1253160 AT3G02940.2 61.798 89 34 0 1 89 1 89 2.81e-36 124
Msa1253160 AT1G16490.1 62.500 88 33 0 2 89 4 91 2.90e-36 123
Msa1253160 AT5G15310.4 64.045 89 32 0 1 89 1 89 4.47e-36 122
Msa1253160 AT2G31180.1 65.169 89 31 0 1 89 1 89 5.35e-36 122
Msa1253160 AT5G16770.4 61.798 89 34 0 1 89 1 89 5.58e-36 124
Msa1253160 AT5G16770.3 61.798 89 34 0 1 89 1 89 5.58e-36 124
Msa1253160 AT3G02940.1 61.798 89 34 0 1 89 1 89 6.24e-36 124
Msa1253160 AT3G02940.3 61.798 89 34 0 1 89 1 89 6.24e-36 124
Msa1253160 AT5G16770.1 61.798 89 34 0 1 89 1 89 8.44e-36 124
Msa1253160 AT5G16770.2 61.798 89 34 0 1 89 1 89 8.44e-36 124
Msa1253160 AT4G21440.1 61.798 89 34 0 1 89 1 89 2.45e-35 123
Msa1253160 AT1G56160.1 61.364 88 34 0 2 89 4 91 4.15e-35 121
Msa1253160 AT5G26660.1 62.921 89 33 0 1 89 1 89 8.31e-35 121
Msa1253160 AT1G34670.1 59.551 89 36 0 1 89 1 89 1.05e-34 121
Msa1253160 AT5G65230.1 60.674 89 35 0 1 89 1 89 1.22e-34 120
Msa1253160 AT5G62320.1 62.105 95 29 1 2 89 3 97 2.42e-34 118
Msa1253160 AT3G01140.1 63.529 85 31 0 5 89 48 132 3.43e-34 120
Msa1253160 AT4G09460.1 59.551 89 36 0 1 89 1 89 3.85e-34 117
Msa1253160 AT1G09540.1 61.798 89 34 0 1 89 1 89 4.82e-34 120
Msa1253160 AT5G10280.1 59.551 89 36 0 1 89 1 89 6.76e-34 119
Msa1253160 AT1G79180.1 61.364 88 34 0 2 89 4 91 6.78e-34 118
Msa1253160 AT1G35515.1 56.180 89 39 0 1 89 1 89 1.63e-33 115
Msa1253160 AT3G12820.1 62.069 87 33 0 3 89 5 91 1.92e-33 115
Msa1253160 AT1G57560.1 59.551 89 36 0 1 89 1 89 2.04e-33 117
Msa1253160 AT4G38620.1 59.551 89 36 0 1 89 1 89 2.47e-33 116
Msa1253160 AT4G17785.1 58.889 90 36 1 1 89 1 90 3.04e-33 117
Msa1253160 AT4G17785.2 58.889 90 36 1 1 89 1 90 3.23e-33 117
Msa1253160 AT5G49330.1 61.798 89 34 0 1 89 1 89 3.29e-33 117
Msa1253160 AT4G05100.1 59.574 94 29 3 1 89 1 90 3.31e-33 117
Msa1253160 AT1G57560.2 59.551 89 36 0 1 89 1 89 4.22e-33 115
Msa1253160 AT3G13540.1 62.353 85 32 0 5 89 16 100 8.45e-33 114
Msa1253160 AT3G47600.1 58.427 89 37 0 1 89 1 89 9.50e-33 115
Msa1253160 AT2G16720.1 57.303 89 38 0 1 89 1 89 1.01e-32 114
Msa1253160 AT3G13890.2 59.551 89 36 0 1 89 1 89 1.01e-32 116
Msa1253160 AT2G47460.1 60.674 89 35 0 1 89 1 89 1.05e-32 116
Msa1253160 AT4G01680.1 60.674 89 35 0 1 89 1 89 1.77e-32 115
Msa1253160 AT5G62470.2 57.303 89 38 0 1 89 1 89 2.52e-32 115
Msa1253160 AT1G08810.1 58.427 89 37 0 1 89 1 89 4.95e-32 112
Msa1253160 AT3G62610.1 58.427 89 37 0 1 89 1 89 5.01e-32 114
Msa1253160 AT1G22640.1 56.180 89 39 0 1 89 1 89 6.64e-32 112
Msa1253160 AT4G01680.3 60.674 89 35 0 1 89 1 89 1.36e-31 110
Msa1253160 AT5G61420.2 53.933 89 41 0 1 89 1 89 2.04e-31 113
Msa1253160 AT5G07690.1 55.056 89 40 0 1 89 1 89 4.09e-31 111
Msa1253160 AT4G34990.1 57.303 89 38 0 1 89 1 89 4.91e-31 110
Msa1253160 AT1G74650.1 57.303 89 38 0 1 89 1 89 4.98e-31 111
Msa1253160 AT5G62470.1 57.303 89 37 1 1 89 1 88 5.72e-31 111
Msa1253160 AT5G60890.1 53.933 89 41 0 1 89 1 89 6.54e-31 110
Msa1253160 AT3G28910.1 58.427 89 37 0 1 89 1 89 1.01e-30 110
Msa1253160 AT1G74080.1 56.180 89 39 0 1 89 1 89 1.50e-30 110
Msa1253160 AT5G56110.1 58.427 89 37 0 1 89 1 89 3.67e-30 108
Msa1253160 AT3G13890.1 54.082 98 36 1 1 89 1 98 6.75e-30 108
Msa1253160 AT3G12720.1 58.427 89 36 1 1 89 12 99 8.18e-30 107
Msa1253160 AT3G49690.1 53.191 94 35 2 1 89 1 90 8.90e-30 107
Msa1253160 AT5G65790.1 52.128 94 36 2 1 89 1 90 1.32e-29 108
Msa1253160 AT3G28470.1 52.688 93 36 2 1 89 1 89 1.41e-29 107
Msa1253160 AT1G18570.1 55.556 90 39 1 1 89 1 90 2.79e-29 107
Msa1253160 AT4G01680.2 53.465 101 35 1 1 89 1 101 3.25e-29 107
Msa1253160 AT5G07700.1 52.809 89 42 0 1 89 1 89 4.04e-29 106
Msa1253160 AT5G07700.2 52.809 89 42 0 1 89 1 89 4.04e-29 106
Msa1253160 AT4G37780.1 51.064 94 37 2 1 89 1 90 9.63e-29 104
Msa1253160 AT1G63910.1 53.933 89 41 0 1 89 1 89 1.29e-28 105
Msa1253160 AT5G23000.1 54.255 94 34 2 1 89 1 90 2.68e-28 104
Msa1253160 AT5G54230.1 55.056 89 40 0 1 89 1 89 3.31e-28 103
Msa1253160 AT3G01530.1 57.692 78 33 0 12 89 25 102 7.49e-28 100
Msa1253160 AT1G79180.2 46.281 121 32 2 2 89 4 124 1.19e-27 102
Msa1253160 AT5G57620.1 51.064 94 37 2 1 89 1 90 1.49e-27 102
Msa1253160 AT1G74430.1 56.989 93 32 2 1 89 1 89 3.39e-27 100
Msa1253160 AT2G36890.1 52.128 94 36 2 1 89 1 90 3.47e-27 100
Msa1253160 AT1G18710.1 55.056 89 40 0 1 89 1 89 4.24e-27 99.8
Msa1253160 AT2G36890.2 52.128 94 36 2 1 89 42 131 4.45e-27 101
Msa1253160 AT5G12870.1 60.256 78 31 0 12 89 18 95 1.90e-26 98.6
Msa1253160 AT3G08500.1 56.098 82 36 0 8 89 26 107 1.39e-25 97.1
Msa1253160 AT1G68320.1 53.846 78 36 0 12 89 19 96 1.48e-25 96.3
Msa1253160 AT3G27810.1 53.846 78 36 0 12 89 20 97 3.69e-25 94.0
Msa1253160 AT5G40350.1 52.564 78 37 0 12 89 17 94 7.36e-25 92.8
Msa1253160 AT2G32460.1 54.217 83 30 2 11 89 17 95 1.54e-24 95.5
Msa1253160 AT2G32460.2 54.217 83 30 2 11 89 5 83 1.97e-24 95.5
Msa1253160 AT2G26960.1 50.588 85 34 2 9 89 17 97 2.29e-24 94.7
Msa1253160 AT5G55020.1 55.000 80 36 0 10 89 24 103 2.46e-24 95.1
Msa1253160 AT5G06100.1 47.561 82 43 0 8 89 28 109 2.27e-23 92.4
Msa1253160 AT3G06490.1 53.659 82 38 0 8 89 15 96 2.68e-23 90.9
Msa1253160 AT3G24310.1 53.165 79 37 0 11 89 17 95 3.16e-23 89.7
Msa1253160 AT3G27920.1 51.948 77 37 0 13 89 15 91 3.43e-23 89.0
Msa1253160 AT5G06100.5 47.561 82 43 0 8 89 28 109 4.16e-23 91.7
Msa1253160 AT5G06100.2 47.561 82 43 0 8 89 28 109 4.16e-23 91.7
Msa1253160 AT5G06100.3 47.561 82 43 0 8 89 28 109 4.16e-23 91.7
Msa1253160 AT5G06100.4 47.561 82 43 0 8 89 28 109 4.16e-23 91.7
Msa1253160 AT5G14750.1 53.247 77 36 0 13 89 17 93 5.83e-23 87.8
Msa1253160 AT4G13480.1 54.545 77 35 0 13 89 7 83 6.44e-23 89.0
Msa1253160 AT4G26930.1 51.190 84 33 2 10 89 17 96 7.80e-23 90.5
Msa1253160 AT3G11440.2 47.436 78 41 0 12 89 41 118 8.68e-23 90.9
Msa1253160 AT3G11440.3 47.436 78 41 0 12 89 41 118 1.02e-22 90.9
Msa1253160 AT3G11440.4 47.436 78 41 0 12 89 41 118 1.02e-22 90.9
Msa1253160 AT3G11440.1 47.436 78 41 0 12 89 41 118 1.02e-22 90.9
Msa1253160 AT3G30210.1 49.367 79 40 0 11 89 26 104 2.25e-22 87.8
Msa1253160 AT1G48000.1 50.000 80 40 0 10 89 30 109 2.90e-22 86.7
Msa1253160 AT5G40330.1 50.649 77 38 0 13 89 13 89 4.07e-22 85.9
Msa1253160 AT5G52260.1 58.442 77 32 0 13 89 13 89 1.18e-21 85.5
Msa1253160 AT2G47190.1 48.718 78 40 0 12 89 20 97 1.29e-21 85.5
Msa1253160 AT1G25340.1 49.351 77 39 0 13 89 19 95 3.94e-21 84.3
Msa1253160 AT4G25560.1 53.247 77 36 0 13 89 11 87 4.85e-21 84.3
Msa1253160 AT3G46130.1 50.633 79 39 0 11 89 8 86 1.37e-20 82.8
Msa1253160 AT5G59780.3 51.948 77 37 0 13 89 9 85 1.76e-20 82.0
Msa1253160 AT5G35550.1 52.564 78 37 0 12 89 14 91 4.04e-20 81.3
Msa1253160 AT5G26660.2 77.778 45 10 0 45 89 14 58 1.33e-19 80.9
Msa1253160 AT5G49620.1 46.341 82 44 0 8 89 22 103 3.01e-19 79.7
Msa1253160 AT5G49620.2 46.250 80 43 0 8 87 22 101 7.14e-19 79.0
Msa1253160 AT3G53200.1 51.220 82 32 2 12 89 9 86 3.82e-18 75.9
Msa1253160 AT3G48920.1 46.753 77 41 0 13 89 19 95 5.85e-18 75.9
Msa1253160 AT5G52600.1 45.679 81 44 0 9 89 9 89 5.90e-18 74.7
Msa1253160 AT1G66380.2 51.899 79 38 0 11 89 7 85 1.11e-17 71.6
Msa1253160 AT2G26950.1 40.741 81 48 0 9 89 13 93 2.72e-17 75.1
Msa1253160 AT1G66370.1 46.067 89 44 1 1 89 1 85 3.35e-17 73.6
Msa1253160 AT1G25340.2 45.455 77 37 1 13 89 19 90 7.14e-17 73.2
Msa1253160 AT1G25340.3 45.455 77 37 1 13 89 19 90 9.58e-17 73.2
Msa1253160 AT5G14750.3 72.093 43 12 0 47 89 17 59 1.44e-16 70.5
Msa1253160 AT1G66380.1 50.633 79 39 0 11 89 7 85 1.81e-16 69.3
Msa1253160 AT3G12820.2 69.767 43 13 0 47 89 1 43 1.15e-15 68.6
Msa1253160 AT3G47600.2 66.667 45 15 0 45 89 39 83 1.56e-15 69.7
Msa1253160 AT3G60460.1 44.048 84 36 4 12 89 11 89 5.62e-15 68.2
Msa1253160 AT2G23290.1 41.250 80 38 2 14 89 13 87 6.66e-15 68.2
Msa1253160 AT5G58850.1 42.683 82 38 3 12 89 103 179 1.02e-14 67.8
Msa1253160 AT1G56650.1 49.367 79 40 0 11 89 7 85 1.22e-14 66.6
Msa1253160 AT3G09230.1 41.250 80 38 2 14 89 55 129 2.60e-14 66.6
Msa1253160 AT4G37260.1 38.750 80 40 2 14 89 13 87 6.46e-14 65.5
Msa1253160 AT5G11050.1 40.000 80 39 3 14 89 105 179 6.66e-14 65.5
Msa1253160 AT5G40350.2 60.000 45 18 0 45 89 5 49 7.86e-14 63.2
Msa1253160 AT1G66390.1 49.367 79 40 0 11 89 7 85 1.22e-13 63.9
Msa1253160 AT1G69560.1 41.176 85 41 3 9 89 102 181 1.54e-13 64.3
Msa1253160 AT1G69560.2 41.176 85 41 3 9 89 118 197 1.56e-13 64.3
Msa1253160 AT3G27810.2 60.000 45 18 0 45 89 4 48 1.78e-13 62.4
Msa1253160 AT5G35550.2 60.000 50 20 0 40 89 45 94 2.49e-13 63.2
Msa1253160 AT3G55730.1 41.250 80 38 2 14 89 56 130 3.77e-13 63.5
Msa1253160 AT5G67300.1 36.250 80 42 2 14 89 6 80 5.47e-13 62.8
Msa1253160 AT3G46130.3 57.447 47 20 0 43 89 8 54 5.56e-13 62.0
Msa1253160 AT4G18770.1 36.471 85 45 2 9 89 212 291 7.54e-13 62.8
Msa1253160 AT3G50060.1 35.000 80 43 2 14 89 6 80 1.59e-12 61.6
Msa1253160 AT3G46130.4 60.000 45 18 0 45 89 20 64 1.60e-12 60.8
Msa1253160 AT3G27785.1 38.824 85 43 3 9 89 184 263 1.61e-12 61.6
Msa1253160 AT5G59780.2 60.000 45 18 0 45 89 20 64 1.79e-12 60.5
Msa1253160 AT1G26780.2 42.683 82 38 3 12 89 96 172 1.82e-12 61.6
Msa1253160 AT1G26780.1 42.683 82 38 3 12 89 96 172 2.69e-12 60.8
Msa1253160 AT3G53200.2 62.222 45 17 0 45 89 9 53 5.79e-12 58.9
Msa1253160 AT3G29020.2 35.000 80 43 2 14 89 67 141 1.52e-11 58.9
Msa1253160 AT3G09370.3 34.146 82 45 2 12 89 141 217 2.93e-11 58.2
Msa1253160 AT3G09370.1 34.146 82 45 2 12 89 128 204 2.95e-11 58.2
Msa1253160 AT3G09370.2 34.146 82 45 2 12 89 133 209 2.96e-11 58.2
Msa1253160 AT2G39880.1 40.000 80 39 2 14 89 50 124 3.85e-11 57.8
Msa1253160 AT1G17950.1 41.975 81 38 3 13 89 4 79 5.32e-11 57.0

Find 37 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACTTTATCCTTAATAAT+TGG 0.145066 8_2:-50412797 Msa1253160:CDS
CTTTAGAGGTTCAAAGAAAA+TGG 0.188117 8_2:-50412892 Msa1253160:exon
TGAAAATCTGATGTATATTT+AGG 0.234005 8_2:-50412504 Msa1253160:three_prime_UTR
ACACTGAATCCCAATTATTA+AGG 0.311416 8_2:+50412787 None:intergenic
TTTAGGAGGAATTTCATGTT+TGG 0.314014 8_2:-50412487 Msa1253160:three_prime_UTR
AAATATTTACCTGCAAGTTT+AGG 0.328128 8_2:+50412753 None:intergenic
ACCAAGTAATAGAGTCTCTT+TGG 0.330399 8_2:-50412438 Msa1253160:three_prime_UTR
GAGAATGTTCCTAAACTTGC+AGG 0.336754 8_2:-50412762 Msa1253160:intron
TTAGGAGGAATTTCATGTTT+GGG 0.344704 8_2:-50412486 Msa1253160:three_prime_UTR
CAATAAAGCCACCTCTCTTA+AGG 0.350479 8_2:+50412580 None:intergenic
GAGAGGTGGCTTTATTGAAA+TGG 0.356209 8_2:-50412574 Msa1253160:CDS
GGAATTTCATGTTTGGGGTT+GGG 0.359981 8_2:-50412480 Msa1253160:three_prime_UTR
TACACGCCCGTCTTGGTAAC+AGG 0.368084 8_2:-50412530 Msa1253160:CDS
GAACTTTATCCTTAATAATT+GGG 0.368286 8_2:-50412796 Msa1253160:CDS
TTCAAAGGTAAAGACTTTAG+AGG 0.378092 8_2:-50412906 Msa1253160:five_prime_UTR
AGGAATTTCATGTTTGGGGT+TGG 0.391112 8_2:-50412481 Msa1253160:three_prime_UTR
TTTAGAGGTTCAAAGAAAAT+GGG 0.440701 8_2:-50412891 Msa1253160:exon
TGAGATGGATTAATTATTTG+AGG 0.448610 8_2:-50412608 Msa1253160:CDS
TCAATACCTGTTACCAAGAC+GGG 0.457645 8_2:+50412524 None:intergenic
TGATCTTCTTCAATAGTCCA+TGG 0.502122 8_2:+50412828 None:intergenic
AAGCCACCTCTCTTAAGGTC+TGG 0.512429 8_2:+50412585 None:intergenic
TAATAATTGGGATTCAGTGT+TGG 0.522517 8_2:-50412784 Msa1253160:CDS
ATACAACTACACGCCCGTCT+TGG 0.530937 8_2:-50412537 Msa1253160:CDS
TTTGAAGGTCTTCTAAGATG+TGG 0.533534 8_2:-50412645 Msa1253160:intron
TGGCTTTATTGAAATGGAAG+AGG 0.537114 8_2:-50412568 Msa1253160:CDS
ACCAAAGAGACTCTATTACT+TGG 0.546008 8_2:+50412437 None:intergenic
AAATCTGATGTATATTTAGG+AGG 0.552362 8_2:-50412501 Msa1253160:three_prime_UTR
CAACCATGTTGTGATAAGGT+TGG 0.554200 8_2:-50412864 Msa1253160:CDS
TAGGAGGAATTTCATGTTTG+GGG 0.556808 8_2:-50412485 Msa1253160:three_prime_UTR
GAAAGAGTTGTAGATTGAGA+TGG 0.557753 8_2:-50412623 Msa1253160:CDS
GATAAGGTTGGTTTGAAGAG+AGG 0.562058 8_2:-50412852 Msa1253160:CDS
AGGCCAGACCTTAAGAGAGG+TGG 0.572119 8_2:-50412588 Msa1253160:CDS
TTCAATACCTGTTACCAAGA+CGG 0.573621 8_2:+50412523 None:intergenic
AAGACAACCATGTTGTGATA+AGG 0.590627 8_2:-50412868 Msa1253160:CDS
TTGAGGCCAGACCTTAAGAG+AGG 0.617024 8_2:-50412591 Msa1253160:CDS
AAACCAACCTTATCACAACA+TGG 0.641918 8_2:+50412861 None:intergenic
TTGGTTTGAAGAGAGGACCA+TGG 0.663165 8_2:-50412845 Msa1253160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATATCTTGATATTTTCAA+AGG - chr8_2:50412438-50412457 Msa1253160:three_prime_UTR 20.0%
!! TGAACTTTATCCTTAATAAT+TGG - chr8_2:50412562-50412581 Msa1253160:CDS 20.0%
!! GAACTTTATCCTTAATAATT+GGG - chr8_2:50412563-50412582 Msa1253160:CDS 20.0%
!! TGAAAATCTGATGTATATTT+AGG - chr8_2:50412855-50412874 Msa1253160:CDS 20.0%
! TTTAGAGGTTCAAAGAAAAT+GGG - chr8_2:50412468-50412487 Msa1253160:three_prime_UTR 25.0%
! AAATATTTACCTGCAAGTTT+AGG + chr8_2:50412609-50412628 None:intergenic 25.0%
!!! TGAGATGGATTAATTATTTG+AGG - chr8_2:50412751-50412770 Msa1253160:intron 25.0%
! AAATCTGATGTATATTTAGG+AGG - chr8_2:50412858-50412877 Msa1253160:CDS 25.0%
TTCAAAGGTAAAGACTTTAG+AGG - chr8_2:50412453-50412472 Msa1253160:three_prime_UTR 30.0%
! CTTTAGAGGTTCAAAGAAAA+TGG - chr8_2:50412467-50412486 Msa1253160:three_prime_UTR 30.0%
ACACTGAATCCCAATTATTA+AGG + chr8_2:50412575-50412594 None:intergenic 30.0%
TAATAATTGGGATTCAGTGT+TGG - chr8_2:50412575-50412594 Msa1253160:CDS 30.0%
!! AACATGCTGCATATTTTTGA+AGG - chr8_2:50412699-50412718 Msa1253160:intron 30.0%
TTTAGGAGGAATTTCATGTT+TGG - chr8_2:50412872-50412891 Msa1253160:CDS 30.0%
TTAGGAGGAATTTCATGTTT+GGG - chr8_2:50412873-50412892 Msa1253160:CDS 30.0%
AAGACAACCATGTTGTGATA+AGG - chr8_2:50412491-50412510 Msa1253160:three_prime_UTR 35.0%
AAACCAACCTTATCACAACA+TGG + chr8_2:50412501-50412520 None:intergenic 35.0%
TGATCTTCTTCAATAGTCCA+TGG + chr8_2:50412534-50412553 None:intergenic 35.0%
!! TTTGAAGGTCTTCTAAGATG+TGG - chr8_2:50412714-50412733 Msa1253160:intron 35.0%
GAAAGAGTTGTAGATTGAGA+TGG - chr8_2:50412736-50412755 Msa1253160:intron 35.0%
!! TGGCTTTATTGAAATGGAAG+AGG - chr8_2:50412791-50412810 Msa1253160:CDS 35.0%
TTCAATACCTGTTACCAAGA+CGG + chr8_2:50412839-50412858 None:intergenic 35.0%
TAGGAGGAATTTCATGTTTG+GGG - chr8_2:50412874-50412893 Msa1253160:CDS 35.0%
ACCAAGTAATAGAGTCTCTT+TGG - chr8_2:50412921-50412940 Msa1253160:five_prime_UTR 35.0%
ACCAAAGAGACTCTATTACT+TGG + chr8_2:50412925-50412944 None:intergenic 35.0%
CAACCATGTTGTGATAAGGT+TGG - chr8_2:50412495-50412514 Msa1253160:three_prime_UTR 40.0%
!! GATAAGGTTGGTTTGAAGAG+AGG - chr8_2:50412507-50412526 Msa1253160:exon 40.0%
GAGAATGTTCCTAAACTTGC+AGG - chr8_2:50412597-50412616 Msa1253160:CDS 40.0%
CAATAAAGCCACCTCTCTTA+AGG + chr8_2:50412782-50412801 None:intergenic 40.0%
!! GAGAGGTGGCTTTATTGAAA+TGG - chr8_2:50412785-50412804 Msa1253160:CDS 40.0%
TCAATACCTGTTACCAAGAC+GGG + chr8_2:50412838-50412857 None:intergenic 40.0%
! AGGAATTTCATGTTTGGGGT+TGG - chr8_2:50412878-50412897 Msa1253160:exon 40.0%
! GGAATTTCATGTTTGGGGTT+GGG - chr8_2:50412879-50412898 Msa1253160:exon 40.0%
! TTGGTTTGAAGAGAGGACCA+TGG - chr8_2:50412514-50412533 Msa1253160:exon 45.0%
TTGAGGCCAGACCTTAAGAG+AGG - chr8_2:50412768-50412787 Msa1253160:CDS 50.0%
AAGCCACCTCTCTTAAGGTC+TGG + chr8_2:50412777-50412796 None:intergenic 50.0%
ATACAACTACACGCCCGTCT+TGG - chr8_2:50412822-50412841 Msa1253160:CDS 50.0%
AGGCCAGACCTTAAGAGAGG+TGG - chr8_2:50412771-50412790 Msa1253160:CDS 55.0%
! TACACGCCCGTCTTGGTAAC+AGG - chr8_2:50412829-50412848 Msa1253160:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 50412437 50412944 50412437 ID=Msa1253160;Name=Msa1253160
chr8_2 mRNA 50412437 50412944 50412437 ID=Msa1253160-mRNA-1;Parent=Msa1253160;Name=Msa1253160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=49|1|1|1|1|1|2|87|89
chr8_2 exon 50412437 50412660 50412437 ID=Msa1253160-mRNA-1:exon:15361;Parent=Msa1253160-mRNA-1
chr8_2 exon 50412763 50412944 50412763 ID=Msa1253160-mRNA-1:exon:15360;Parent=Msa1253160-mRNA-1
chr8_2 five_prime_UTR 50412896 50412944 50412896 ID=Msa1253160-mRNA-1:five_prime_utr;Parent=Msa1253160-mRNA-1
chr8_2 CDS 50412763 50412895 50412763 ID=Msa1253160-mRNA-1:cds;Parent=Msa1253160-mRNA-1
chr8_2 CDS 50412524 50412660 50412524 ID=Msa1253160-mRNA-1:cds;Parent=Msa1253160-mRNA-1
chr8_2 three_prime_UTR 50412437 50412523 50412437 ID=Msa1253160-mRNA-1:three_prime_utr;Parent=Msa1253160-mRNA-1
Gene Sequence

>Msa1253160

ATGGGAAGACAACCATGTTGTGATAAGGTTGGTTTGAAGAGAGGACCATGGACTATTGAAGAAGATCACAAGCTTGTGAACTTTATCCTTAATAATTGGGATTCAGTGTTGGAGAATGTTCCTAAACTTGCAGGTCTTCTAAGATGTGGAAAGAGTTGTAGATTGAGATGGATTAATTATTTGAGGCCAGACCTTAAGAGAGGTGGCTTTATTGAAATGGAAGAGGATCAAATTATACAACTACACGCCCGTCTTGGTAACAGGTATTGA
Protein sequence

>Msa1253160

MGRQPCCDKVGLKRGPWTIEEDHKLVNFILNNWDSVLENVPKLAGLLRCGKSCRLRWINYLRPDLKRGGFIEMEEDQIIQLHARLGNRY*