AlfalfaGEDB | Msa1253830

M. sativa cultivar zhongmu-4 / Msa1253830

KEGG: K00924|K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009909 GO:0009910 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048519 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0060089 GO:0060860 GO:0060862 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:2000026 GO:2000241 GO:2000242
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253830	XP_013446329.3	97.555	409	9	1	1	409	1	408	0.0	816

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253830	sp\|Q9SII6\|PIX13_ARATH	68.047	338	106	1	68	405	62	397	1.56e-170	486

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253830	A0A396GMV8	97.555	409	9	1	1	409	1	408	0.0	816

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253830	MtrunA17_Chr8g0370461	97.555	409	9	1	1	409	1	408	0.0	816
Msa1253830	MtrunA17_Chr8g0370571	86.327	373	26	4	32	403	18	366	0.0	643
Msa1253830	MtrunA17_Chr5g0406841	67.574	404	123	4	1	400	56	455	0.0	531
Msa1253830	MtrunA17_Chr3g0143951	63.372	344	121	2	53	394	42	382	9.01e-155	443
Msa1253830	MtrunA17_Chr2g0328171	52.538	394	166	4	1	392	1	375	6.47e-154	441
Msa1253830	MtrunA17_Chr5g0435211	52.987	385	163	3	1	385	1	367	4.79e-152	436
Msa1253830	MtrunA17_Chr5g0416311	61.446	332	126	1	63	394	46	375	1.62e-151	435
Msa1253830	MtrunA17_Chr5g0448661	64.263	319	109	1	67	385	79	392	3.74e-150	431
Msa1253830	MtrunA17_Chr3g0111631	50.123	407	179	4	1	407	3	385	9.28e-145	417
Msa1253830	MtrunA17_Chr7g0238671	58.696	322	133	0	70	391	50	371	2.52e-143	414

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1253830	AT2G17220.1	68.047	338	106	1	68	405	62	397	1.58e-171	486
Msa1253830	AT2G17220.2	68.047	338	106	1	68	405	61	396	1.70e-171	486
Msa1253830	AT4G35600.1	63.158	361	118	2	29	389	34	379	1.59e-170	484
Msa1253830	AT4G35600.2	63.158	361	118	2	29	389	35	380	1.87e-170	483
Msa1253830	AT2G28930.2	61.709	316	121	0	66	381	42	357	1.57e-151	435
Msa1253830	AT2G28930.3	61.709	316	121	0	66	381	45	360	2.31e-151	435
Msa1253830	AT2G28930.1	61.709	316	121	0	66	381	53	368	2.97e-151	435
Msa1253830	AT1G07570.1	61.635	318	122	0	64	381	39	356	3.70e-151	434
Msa1253830	AT1G07570.5	61.635	318	122	0	64	381	39	356	3.70e-151	434
Msa1253830	AT1G07570.2	61.635	318	122	0	64	381	39	356	3.70e-151	434

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Find 115 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTTATAAAGGTTGGT+TGG	0.474192	8_2:+51560273	Msa1253830:CDS
AAAATCATAGAATAGTTAAG+AGG	0.477918	8_2:-51564412	None:intergenic
AAACGGAGAGAACTTCACAT+GGG	0.748265	8_2:+51559781	Msa1253830:exon
AAGAGTGATGTGTATGGCTT+TGG	0.465372	8_2:+51563905	Msa1253830:CDS
AAGGCTCTCTCACCCAAACT+TGG	0.506037	8_2:+51561948	Msa1253830:CDS
AATATTTCGCATATACAATA+TGG	0.307463	8_2:+51564472	Msa1253830:three_prime_UTR
ACAAAGAGAGAAGTATTAAT+GGG	0.388680	8_2:-51559731	None:intergenic
ACAACAAAACCTAGTTGAAT+GGG	0.498972	8_2:+51563988	Msa1253830:CDS
ACAAGAATCAAAATAGCTAT+TGG	0.431182	8_2:+51562190	Msa1253830:CDS
ACAATCACATGTAACTACCA+GGG	0.708704	8_2:+51562877	Msa1253830:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGCAATTGTCCAATGAATT+TGG	-	chr8_2:51559757-51559776	None:intergenic	30.0%
AAGATGGGTGATTTAGAAAT+TGG	-	chr8_2:51559898-51559917	None:intergenic	30.0%
CTTTCAAGAATTTGTCCATT+TGG	-	chr8_2:51560163-51560182	None:intergenic	30.0%
TCTTGAAAGACCAAATTTGA+AGG	+	chr8_2:51560174-51560193	Msa1253830:CDS	30.0%
GTCCATAAAATTGTAGATTG+GGG	-	chr8_2:51560780-51560799	None:intergenic	30.0%
TGAAAATGTGGAAGATTGTT+TGG	+	chr8_2:51560838-51560857	Msa1253830:intron	30.0%
AAAATCAAGAGTTTGGAAGA+TGG	+	chr8_2:51561061-51561080	Msa1253830:intron	30.0%
GATAATTAATGGAAGGAAGA+AGG	-	chr8_2:51561148-51561167	None:intergenic	30.0%
ACTCAAGGATAATTAATGGA+AGG	-	chr8_2:51561155-51561174	None:intergenic	30.0%
CTTGACTCAAGGATAATTAA+TGG	-	chr8_2:51561159-51561178	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	51559587	51564537	51559587	ID=Msa1253830;Name=Msa1253830
chr8_2	mRNA	51559587	51564537	51559587	ID=Msa1253830-mRNA-1;Parent=Msa1253830;Name=Msa1253830-mRNA-1;_AED=0.09;_eAED=0.09;_QI=212\|1\|1\|1\|1\|1\|6\|255\|409
chr8_2	exon	51559587	51559877	51559587	ID=Msa1253830-mRNA-1:exon:15796;Parent=Msa1253830-mRNA-1
chr8_2	exon	51560038	51560393	51560038	ID=Msa1253830-mRNA-1:exon:15797;Parent=Msa1253830-mRNA-1
chr8_2	exon	51561928	51562063	51561928	ID=Msa1253830-mRNA-1:exon:15798;Parent=Msa1253830-mRNA-1
chr8_2	exon	51562158	51562300	51562158	ID=Msa1253830-mRNA-1:exon:15799;Parent=Msa1253830-mRNA-1
chr8_2	exon	51562821	51562944	51562821	ID=Msa1253830-mRNA-1:exon:15800;Parent=Msa1253830-mRNA-1
chr8_2	exon	51563891	51564537	51563891	ID=Msa1253830-mRNA-1:exon:15801;Parent=Msa1253830-mRNA-1
chr8_2	five_prime_UTR	51559587	51559798	51559587	ID=Msa1253830-mRNA-1:five_prime_utr;Parent=Msa1253830-mRNA-1
chr8_2	CDS	51559799	51559877	51559799	ID=Msa1253830-mRNA-1:cds;Parent=Msa1253830-mRNA-1
chr8_2	CDS	51560038	51560393	51560038	ID=Msa1253830-mRNA-1:cds;Parent=Msa1253830-mRNA-1
chr8_2	CDS	51561928	51562063	51561928	ID=Msa1253830-mRNA-1:cds;Parent=Msa1253830-mRNA-1
chr8_2	CDS	51562158	51562300	51562158	ID=Msa1253830-mRNA-1:cds;Parent=Msa1253830-mRNA-1
chr8_2	CDS	51562821	51562944	51562821	ID=Msa1253830-mRNA-1:cds;Parent=Msa1253830-mRNA-1
chr8_2	CDS	51563891	51564282	51563891	ID=Msa1253830-mRNA-1:cds;Parent=Msa1253830-mRNA-1
chr8_2	three_prime_UTR	51564283	51564537	51564283	ID=Msa1253830-mRNA-1:three_prime_utr;Parent=Msa1253830-mRNA-1

Gene Sequence

>Msa1253830

ATGGGTCTCTGTTTTTCCTTTCTCTGCCCCTCCTCTGCACCCCCTCAATCTTCTTCCTTCAACAGACCTCTTTCTTCAGGTTCAACAAGCACTGTTGGATTCTCAGCAACTACAAGCAGTGCTGGAAGAAGCCAATTCTCTGAGGTAGCAAGTGGAAGCATTGATGATACTAATGAAGGAGGATCTCTTCCTTATCCTTCACCAAATGGACAAATTCTTGAAAGACCAAATTTGAAGGTTTTTAGTTTTATTGAATTGAAAGCTGCTACAAAAAGCTTCAAATCTGATACATTACTTGGTGAAGGTGGTTTTGGAAAAGTTTATAAAGGTTGGTTGGATGAGAAAACTCTTTCTCCTGCTAAAGCTGGTTCTGGAATGGTTGTTGCTATCAAAAAATTGAACTCTGAAAGTACTCAAGGATTTCAAGAATGGCAGTCAGAAGTTAACTTCTTAGGAAGGCTCTCTCACCCAAACTTGGTGAAGCTATTAGGTTATTGTTGGGACGACGACGAGCTTCTTCTTGTCTACGAGTTCATGCCAAAAGGAAGTCTGGAGAATCATCTCTTCAGAAGAAATCCTAACATTGAACCACTTTCTTGGAACACAAGAATCAAAATAGCTATTGGTGCAGCTCGTGGTTTGGCTTTCTTGCATGATTCAGCACAGCAAGTCATATACAGAGATTTTAAGGCTTCAAATATACTCCTTGATGGGAGTTACAATGCAAAAATCTCAGATTTCGGATTAGCTAAATTGGGGCCTTCTGGGGGACAATCACATGTAACTACCAGGGTCATGGGGACTTATGGTTATGCTGCTCCAGAATACATTGCAACAGGGCACTTGTATGTGAAGAGTGATGTGTATGGCTTTGGTGTGGTGCTGTTAGAAATCCTAACAGCCATGAGGGCATTGGACACAAAGAGGCCAACAGGACAACAAAACCTAGTTGAATGGGTGAAACCATTTCTGTCAAATAAGAAAAAGTTGAAAGGCATAATGGATGCTAGAATAGAAGGACAATACTCACCAAAGGCAGCAGTACAAGCAGCAGCACTTTCTCTAAAGTGCTTAGAAAATGACCCTAAACAACGTCCTTCTATGAAAGAAGTGCTTGAATCATTGGAAGCTATTGAAGCAATTCAGGTCAAAACCAAAAGATCCAACAACAATTCTCATCAACCTCCAGTTCTTCAAGCTGCTAGGCACCAGAGAGTTGTGAAAGTATAA

Protein sequence

>Msa1253830

MGLCFSFLCPSSAPPQSSSFNRPLSSGSTSTVGFSATTSSAGRSQFSEVASGSIDDTNEGGSLPYPSPNGQILERPNLKVFSFIELKAATKSFKSDTLLGEGGFGKVYKGWLDEKTLSPAKAGSGMVVAIKKLNSESTQGFQEWQSEVNFLGRLSHPNLVKLLGYCWDDDELLLVYEFMPKGSLENHLFRRNPNIEPLSWNTRIKIAIGAARGLAFLHDSAQQVIYRDFKASNILLDGSYNAKISDFGLAKLGPSGGQSHVTTRVMGTYGYAAPEYIATGHLYVKSDVYGFGVVLLEILTAMRALDTKRPTGQQNLVEWVKPFLSNKKKLKGIMDARIEGQYSPKAAVQAAALSLKCLENDPKQRPSMKEVLESLEAIEAIQVKTKRSNNNSHQPPVLQAARHQRVVKV*