AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1254850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1254850 MtrunA17_Chr8g0372461 95.266 338 4 4 1 333 1 331 0.0 648
Msa1254850 MtrunA17_Chr5g0404781 52.646 359 108 14 1 333 1 323 4.74e-89 270
Msa1254850 MtrunA17_Chr3g0142171 43.695 341 126 16 1 328 1 288 4.78e-64 204
Msa1254850 MtrunA17_Chr1g0155791 45.429 361 146 13 1 333 1 338 1.17e-60 197
Msa1254850 MtrunA17_Chr5g0441921 50.000 156 55 4 127 259 124 279 8.69e-41 145
Msa1254850 MtrunA17_Chr4g0035591 49.296 142 62 3 123 263 16 148 1.21e-39 139
Msa1254850 MtrunA17_Chr3g0082511 39.869 153 69 1 127 256 134 286 1.45e-33 126
Msa1254850 MtrunA17_Chr8g0353111 39.884 173 73 3 114 256 111 282 4.44e-33 125
Msa1254850 MtrunA17_Chr0c01g0489181 56.410 39 17 0 123 161 63 101 5.10e-14 67.4
Msa1254850 MtrunA17_Chr2g0281741 61.538 39 15 0 123 161 146 184 3.79e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1254850 AT3G51060.1 44.987 389 139 12 1 333 1 370 2.69e-81 251
Msa1254850 AT5G66350.1 46.070 369 125 14 1 333 1 331 2.82e-76 238
Msa1254850 AT5G66350.3 45.810 358 122 13 1 322 1 322 5.46e-74 231
Msa1254850 AT5G66350.2 45.938 357 121 13 1 321 1 321 1.07e-73 231
Msa1254850 AT1G19790.2 43.935 371 142 20 1 332 1 344 2.45e-68 218
Msa1254850 AT1G19790.1 43.935 371 142 20 1 332 1 344 2.45e-68 218
Msa1254850 AT1G75520.1 42.359 373 148 16 1 333 1 346 1.32e-62 203
Msa1254850 AT4G36260.1 41.176 357 146 14 1 331 1 319 2.14e-53 178
Msa1254850 AT2G21400.2 54.605 152 60 4 124 270 7 154 3.21e-47 158
Msa1254850 AT2G18120.1 53.548 155 57 3 116 261 61 209 3.44e-47 159
Msa1254850 AT2G21400.4 54.605 152 60 4 124 270 6 153 3.78e-47 158
Msa1254850 AT2G21400.3 54.730 148 51 4 124 270 7 139 1.37e-46 156
Msa1254850 AT2G21400.1 54.730 148 51 4 124 270 6 138 1.64e-46 155
Msa1254850 AT2G18120.2 52.288 153 60 2 116 261 61 207 2.09e-46 157
Msa1254850 AT5G12330.1 47.205 161 55 4 127 258 112 271 2.72e-38 139
Msa1254850 AT5G12330.2 47.205 161 55 4 127 258 112 271 2.72e-38 139
Msa1254850 AT5G12330.4 47.205 161 55 4 127 258 199 358 5.21e-38 140
Msa1254850 AT5G33210.1 42.405 158 70 5 93 235 14 165 5.24e-29 110
Msa1254850 AT5G33210.2 61.176 85 23 2 125 199 15 99 4.09e-27 103
Msa1254850 AT3G54430.1 42.553 141 59 6 127 256 41 170 9.96e-26 102
Msa1254850 AT5G12330.3 82.609 46 8 0 127 172 112 157 3.61e-21 90.9

Find 116 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAGAAACTACTGTTATT+AGG 0.143287 8_2:+53140556 Msa1254850:five_prime_UTR
AATGATTGAAACACAAATTT+TGG 0.146618 8_2:-53142377 None:intergenic
CTATTGTTGGTACTACTTTC+TGG 0.159475 8_2:-53142065 None:intergenic
AAAGGAATTCTCTATGATTT+TGG 0.200894 8_2:+53142041 Msa1254850:CDS
AGAAGAAAGAGGAGTTATTA+TGG 0.207274 8_2:+53142310 Msa1254850:three_prime_UTR
ACAGTTGAGAGAGAATTAAT+TGG 0.214572 8_2:-53140450 None:intergenic
TTTCTATGTGTACTCTATAA+TGG 0.229112 8_2:+53142587 Msa1254850:three_prime_UTR
ACAGAGGTAATTTAGAATTA+TGG 0.238498 8_2:+53140733 Msa1254850:CDS
CATGTGAGGACAATCTTTCT+TGG 0.252836 8_2:-53141006 None:intergenic
CACCACAAGAGACACTATTT+TGG 0.271662 8_2:+53140682 Msa1254850:CDS
TAAAGAAACTACTGTTATTA+GGG 0.274863 8_2:+53140557 Msa1254850:five_prime_UTR
TGATCCTTCTTCATTGTATT+CGG 0.283339 8_2:+53142214 Msa1254850:CDS
AATAATAGTAATTACATGAT+TGG 0.330195 8_2:+53142095 Msa1254850:CDS
GAAGAAAGAGGAGTTATTAT+GGG 0.330694 8_2:+53142311 Msa1254850:three_prime_UTR
ATAATAGTAATTACATGATT+GGG 0.347728 8_2:+53142096 Msa1254850:CDS
AACTACTGTTATTAGGGTTA+AGG 0.348943 8_2:+53140563 Msa1254850:five_prime_UTR
AAGTATATGTCTCCTTGTTA+TGG 0.356150 8_2:-53140338 None:intergenic
ATTATTATAATTACTATTGT+TGG 0.366393 8_2:-53142078 None:intergenic
AAGCTATAGCTGCTGCTAGC+TGG 0.376069 8_2:+53140187 Msa1254850:five_prime_UTR
ATGGCTGGTTTGTTCTCATT+AGG 0.380675 8_2:+53140606 Msa1254850:CDS
TAAGCTCACCAGCGGAGTTC+AGG 0.381652 8_2:+53141937 Msa1254850:CDS
TACTCTATAATGGACTTCTA+TGG 0.385956 8_2:+53142597 Msa1254850:three_prime_UTR
CAGCGGAGTTCAGGTGTGTA+AGG 0.390315 8_2:+53141946 Msa1254850:CDS
GTTGTTGTGATTCTTCTCCT+TGG 0.392328 8_2:-53140647 None:intergenic
TACATGATTGGGGAGACTTC+CGG 0.393571 8_2:+53142107 Msa1254850:CDS
TGGCTTGGTTACCACAATCT+TGG 0.393804 8_2:-53140986 None:intergenic
TACGACTGTTGTTGCCTCCT+CGG 0.399669 8_2:+53142181 Msa1254850:CDS
GTTGTTGCTGGTGGTGTTGT+TGG 0.401531 8_2:-53140779 None:intergenic
GTTGTTGGTGCTGTTGTTGT+TGG 0.405595 8_2:-53140764 None:intergenic
CAGTTGAGAGAGAATTAATT+GGG 0.406433 8_2:-53140449 None:intergenic
ATAACTGTTGTTGACGTTCA+CGG 0.415997 8_2:-53141109 None:intergenic
TACGGTCCAGGAGTTGGTTT+AGG 0.417465 8_2:+53140852 Msa1254850:CDS
AGCTATAGCTGCTGCTAGCT+GGG 0.421738 8_2:+53140188 Msa1254850:five_prime_UTR
CCAGGAGTTGGTTTAGGTGT+TGG 0.426741 8_2:+53140858 Msa1254850:CDS
GTTTAGGTGTTGGACCAAGC+AGG 0.429835 8_2:+53140868 Msa1254850:CDS
GCCGTCATAAAGGCATTAAT+CGG 0.438656 8_2:-53142239 None:intergenic
CGTACCACTAGCCGTCATAA+AGG 0.438682 8_2:-53142249 None:intergenic
GGTGCTGTTGTTGTTGGTGT+TGG 0.440333 8_2:-53140758 None:intergenic
TCGTATCAGAGTCGGTGATT+AGG 0.442544 8_2:-53142163 None:intergenic
TATGTCTCCTTGTTATGGTT+GGG 0.444674 8_2:-53140333 None:intergenic
ATAGAAAGAAACAATAGCTT+TGG 0.451472 8_2:-53142571 None:intergenic
ACTTCCGGTGGAGGTGGTGT+TGG 0.461900 8_2:+53142122 Msa1254850:CDS
AACGCCAACACCACCTCCAC+CGG 0.464083 8_2:-53142126 None:intergenic
TTCTTCTTCAAAGAAATGGC+TGG 0.464677 8_2:+53140591 Msa1254850:exon
ATTGGGGAGACTTCCGGTGG+AGG 0.465935 8_2:+53142113 Msa1254850:CDS
TTGTTCTCATTAGGAGGTGG+TGG 0.466253 8_2:+53140615 Msa1254850:CDS
TTTGAAATATCTCTTTGTAA+AGG 0.469835 8_2:-53141140 None:intergenic
GAGATCGGCAGCGTTTACTA+TGG 0.472056 8_2:+53140926 Msa1254850:CDS
GAGGACAATCTTTCTTGGCT+TGG 0.472767 8_2:-53141001 None:intergenic
GGGGAGACTTCCGGTGGAGG+TGG 0.473755 8_2:+53142116 Msa1254850:CDS
TTGAAGGAAGGAAGGAAGGA+AGG 0.475608 8_2:+53140164 Msa1254850:five_prime_UTR
ACGACTGTTGTTGCCTCCTC+GGG 0.479972 8_2:+53142182 Msa1254850:CDS
CAATTGATGATGCAGATGAT+AGG 0.486383 8_2:+53141976 Msa1254850:CDS
TCATTAGGAGGTGGTGGAAG+AGG 0.489984 8_2:+53140621 Msa1254850:CDS
ATATGTCTCCTTGTTATGGT+TGG 0.492221 8_2:-53140334 None:intergenic
AAACTCATGTTAAAAGCACT+TGG 0.502171 8_2:+53141069 Msa1254850:CDS
ACATCAATCGTCGTCGAGAT+CGG 0.503948 8_2:+53140911 Msa1254850:CDS
AAACAAAATTGAGAAGAAAG+AGG 0.504247 8_2:+53142299 Msa1254850:three_prime_UTR
GGTTTGTTCTCATTAGGAGG+TGG 0.504419 8_2:+53140612 Msa1254850:CDS
GCTGGTGAGCTTACCACAGC+AGG 0.505962 8_2:-53141927 None:intergenic
CGTTCACGGCGTCTAGAAGC+AGG 0.507852 8_2:-53141095 None:intergenic
AGGAGTTATTATGGGGATCA+TGG 0.508047 8_2:+53142319 Msa1254850:three_prime_UTR
AAAATGAGATTATTATATGA+AGG 0.508828 8_2:+53142469 Msa1254850:three_prime_UTR
GATCTCTACGGTCCAGGAGT+TGG 0.516227 8_2:+53140846 Msa1254850:CDS
GCTGGTTTGTTCTCATTAGG+AGG 0.517479 8_2:+53140609 Msa1254850:CDS
ATTGGAGGACATTTGTTCAA+AGG 0.517913 8_2:+53142023 Msa1254850:CDS
AGAGCATTTGAACCATCTCT+AGG 0.524317 8_2:-53141167 None:intergenic
AGGTCTTCTTCTTCAGCTGA+AGG 0.529357 8_2:+53140954 Msa1254850:CDS
TCAAATTTACCTCTACTACT+TGG 0.529742 8_2:-53142423 None:intergenic
TTTAGGTGTTGGACCAAGCA+GGG 0.533902 8_2:+53140869 Msa1254850:CDS
GTAAAAGAGAAGTATATATG+TGG 0.544134 8_2:-53142355 None:intergenic
CCAACACCTAAACCAACTCC+TGG 0.554565 8_2:-53140858 None:intergenic
CAAAATAGTGTCTCTTGTGG+TGG 0.555466 8_2:-53140681 None:intergenic
TAGAGATCTCGCGGGAAGAA+CGG 0.556385 8_2:-53140831 None:intergenic
TATCAAACGGCTGTTAACAT+TGG 0.556930 8_2:+53142005 Msa1254850:CDS
TGACCATGACATTGTTGTTG+TGG 0.557428 8_2:+53142400 Msa1254850:three_prime_UTR
TGATAGGTATGCATATCAAA+CGG 0.559733 8_2:+53141992 Msa1254850:CDS
AACAAACGTTGAAGGAAGGA+AGG 0.563270 8_2:+53140156 Msa1254850:five_prime_UTR
CAAACGGCTGTTAACATTGG+AGG 0.566332 8_2:+53142008 Msa1254850:CDS
ATTTCAAAACGCCCTAGAGA+TGG 0.570524 8_2:+53141155 Msa1254850:CDS
TTCTTCCCGCGAGATCTCTA+CGG 0.572634 8_2:+53140834 Msa1254850:CDS
TCATCTGATGAAACCCTGCT+TGG 0.577114 8_2:-53140882 None:intergenic
AACTCATGTTAAAAGCACTT+GGG 0.578823 8_2:+53141070 Msa1254850:CDS
GGGATAAGTTGCCAAGATTG+TGG 0.579161 8_2:+53140975 Msa1254850:CDS
TCCGATTAATGCCTTTATGA+CGG 0.579890 8_2:+53142238 Msa1254850:CDS
TAAAAGAGAAGTATATATGT+GGG 0.583599 8_2:-53142354 None:intergenic
CTTACACACCTGAACTCCGC+TGG 0.583863 8_2:-53141945 None:intergenic
TAATAGTAATTACATGATTG+GGG 0.584644 8_2:+53142097 Msa1254850:CDS
GGTGGTGGAAGAGGAAACCA+AGG 0.593423 8_2:+53140630 Msa1254850:CDS
AATGCCTTTATGACGGCTAG+TGG 0.596750 8_2:+53142245 Msa1254850:CDS
GGTCTTCTTCTTCAGCTGAA+GGG 0.597588 8_2:+53140955 Msa1254850:CDS
AAGAAAGAGGAGTTATTATG+GGG 0.598652 8_2:+53142312 Msa1254850:three_prime_UTR
GAGCATTTGAACCATCTCTA+GGG 0.600057 8_2:-53141166 None:intergenic
AGCGGAGTTCAGGTGTGTAA+GGG 0.600996 8_2:+53141947 Msa1254850:CDS
TTGTTGTGGCCAAGTAGTAG+AGG 0.601864 8_2:+53142414 Msa1254850:three_prime_UTR
AGGATCAACAAGTGCGCCCG+AGG 0.604938 8_2:-53142198 None:intergenic
GGAGCCGAATACAATGAAGA+AGG 0.606433 8_2:-53142218 None:intergenic
AACGTTGAAGGAAGGAAGGA+AGG 0.609399 8_2:+53140160 Msa1254850:five_prime_UTR
GAGTCGGTGATTAGGTTCAA+CGG 0.611827 8_2:-53142155 None:intergenic
ATCAACAAGTGCGCCCGAGG+AGG 0.617141 8_2:-53142195 None:intergenic
AACAACAGTCGTATCAGAGT+CGG 0.625001 8_2:-53142171 None:intergenic
AGAACTTGTTGTAAGAGTCG+TGG 0.626794 8_2:+53141035 Msa1254850:CDS
GGACAGAAAACAGAAACAGA+CGG 0.636265 8_2:-53140263 None:intergenic
GATGTTTCATCTTACAACAG+AGG 0.638713 8_2:+53140717 Msa1254850:CDS
TGCTGTGGTAAGCTCACCAG+CGG 0.638941 8_2:+53141929 Msa1254850:CDS
CCTGGACCGTAGAGATCTCG+CGG 0.642572 8_2:-53140840 None:intergenic
TGGCCACAACAACAATGTCA+TGG 0.643734 8_2:-53142403 None:intergenic
TACCAAAATAGTGTCTCTTG+TGG 0.644271 8_2:-53140684 None:intergenic
CAGCGTTTACTATGGCTATG+AGG 0.650670 8_2:+53140934 Msa1254850:CDS
ATGATTGGGGAGACTTCCGG+TGG 0.658081 8_2:+53142110 Msa1254850:CDS
AAGATGATATGCATGCTGCA+CGG 0.670001 8_2:+53140808 Msa1254850:CDS
GACGCACACAAAAGAAAACA+CGG 0.689183 8_2:+53140400 Msa1254850:five_prime_UTR
CAAGTTCTACATCTCATGTG+AGG 0.701177 8_2:-53141020 None:intergenic
CAGACGGCTAAACAGTGTCG+TGG 0.701923 8_2:-53140247 None:intergenic
CTGGACCGTAGAGATCTCGC+GGG 0.715374 8_2:-53140839 None:intergenic
GGAGAAGAATCACAACAACA+AGG 0.732085 8_2:+53140651 Msa1254850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTAATAATAATAATAA+AGG - chr8_2:53140063-53140082 None:intergenic 0.0%
!! TTAATAATAATAATAAAGGA+AGG - chr8_2:53140059-53140078 None:intergenic 10.0%
!! ATTATTATAATTACTATTGT+TGG - chr8_2:53142081-53142100 None:intergenic 10.0%
!!! AAGAATAAATATAGTATTTT+TGG - chr8_2:53142531-53142550 None:intergenic 10.0%
!! AAATAATAAAAAAAAGCTGA+TGG - chr8_2:53141549-53141568 None:intergenic 15.0%
!! AATAATAGTAATTACATGAT+TGG + chr8_2:53142095-53142114 Msa1254850:CDS 15.0%
!! ATAATAGTAATTACATGATT+GGG + chr8_2:53142096-53142115 Msa1254850:CDS 15.0%
!! AAAATGAGATTATTATATGA+AGG + chr8_2:53142469-53142488 Msa1254850:three_prime_UTR 15.0%
!! TATACAGAAAAACATAAGTT+AGG + chr8_2:53140120-53140139 Msa1254850:five_prime_UTR 20.0%
!! AATTGAAGTTAAGAAAAAGA+AGG - chr8_2:53140287-53140306 None:intergenic 20.0%
!!! ATTTGTGAAAAATGGTTTTT+GGG - chr8_2:53140429-53140448 None:intergenic 20.0%
!!! CTAAATAGGTTTTTTGTTTT+TGG - chr8_2:53140506-53140525 None:intergenic 20.0%
!! CAAAAACAAAAAACCTATTT+AGG + chr8_2:53140504-53140523 Msa1254850:five_prime_UTR 20.0%
!! TTAAAGAAACTACTGTTATT+AGG + chr8_2:53140556-53140575 Msa1254850:five_prime_UTR 20.0%
!! TAAAGAAACTACTGTTATTA+GGG + chr8_2:53140557-53140576 Msa1254850:five_prime_UTR 20.0%
!!! TTTTTTCTTCTTCAAAGAAA+TGG + chr8_2:53140587-53140606 Msa1254850:exon 20.0%
!!! TTTGAAATATCTCTTTGTAA+AGG - chr8_2:53141143-53141162 None:intergenic 20.0%
!!! TTGCTTTTGTTTTTTAGTTA+AGG + chr8_2:53141248-53141267 Msa1254850:intron 20.0%
!!! TGTTTTTCACAAAAGAAAAA+GGG - chr8_2:53141602-53141621 None:intergenic 20.0%
!!! ATGTTTTTCACAAAAGAAAA+AGG - chr8_2:53141603-53141622 None:intergenic 20.0%
!! ATTAAAAAATAAAGGGTTAG+AGG + chr8_2:53141893-53141912 Msa1254850:intron 20.0%
!! TAATAGTAATTACATGATTG+GGG + chr8_2:53142097-53142116 Msa1254850:CDS 20.0%
!!! AATTTTGTTTTCAAGATCTT+GGG - chr8_2:53142289-53142308 None:intergenic 20.0%
!!! TAAAAGAGAAGTATATATGT+GGG - chr8_2:53142357-53142376 None:intergenic 20.0%
!!! AATGATTGAAACACAAATTT+TGG - chr8_2:53142380-53142399 None:intergenic 20.0%
!! GATTTGTGAAAAATGGTTTT+TGG - chr8_2:53140430-53140449 None:intergenic 25.0%
! TAATTGGGATTTGTGAAAAA+TGG - chr8_2:53140437-53140456 None:intergenic 25.0%
!!! ACCTATTTAGGTTTTTGATA+TGG + chr8_2:53140516-53140535 Msa1254850:five_prime_UTR 25.0%
! TCCATATCAAAAACCTAAAT+AGG - chr8_2:53140520-53140539 None:intergenic 25.0%
!! ACAGAGGTAATTTAGAATTA+TGG + chr8_2:53140733-53140752 Msa1254850:CDS 25.0%
! ATTCATATAATAGTACCTGA+AGG - chr8_2:53141212-53141231 None:intergenic 25.0%
!!! TTTGTTTTTTAGTTAAGGAC+TGG + chr8_2:53141253-53141272 Msa1254850:intron 25.0%
!! AAAACAAAGAGAGAATCTTT+TGG + chr8_2:53141392-53141411 Msa1254850:intron 25.0%
! AAAACTTTGTCATCAGTATT+TGG - chr8_2:53141445-53141464 None:intergenic 25.0%
!! AAATACTGATGACAAAGTTT+TGG + chr8_2:53141444-53141463 Msa1254850:intron 25.0%
! AAGAATCACAAAGAAAATGA+TGG - chr8_2:53141737-53141756 None:intergenic 25.0%
!! AAAGGAATTCTCTATGATTT+TGG + chr8_2:53142041-53142060 Msa1254850:CDS 25.0%
!!! CAATTTTGTTTTCAAGATCT+TGG - chr8_2:53142290-53142309 None:intergenic 25.0%
! AAACAAAATTGAGAAGAAAG+AGG + chr8_2:53142299-53142318 Msa1254850:three_prime_UTR 25.0%
!! GTAAAAGAGAAGTATATATG+TGG - chr8_2:53142358-53142377 None:intergenic 25.0%
! ATAGAAAGAAACAATAGCTT+TGG - chr8_2:53142574-53142593 None:intergenic 25.0%
! TTTCTATGTGTACTCTATAA+TGG + chr8_2:53142587-53142606 Msa1254850:three_prime_UTR 25.0%
TACACAAAAACAAACGTTGA+AGG + chr8_2:53140148-53140167 Msa1254850:five_prime_UTR 30.0%
AAGTATATGTCTCCTTGTTA+TGG - chr8_2:53140341-53140360 None:intergenic 30.0%
! CTGTGTTTTGTGTAATTTTC+TGG + chr8_2:53140376-53140395 Msa1254850:five_prime_UTR 30.0%
CAGTTGAGAGAGAATTAATT+GGG - chr8_2:53140452-53140471 None:intergenic 30.0%
ACAGTTGAGAGAGAATTAAT+TGG - chr8_2:53140453-53140472 None:intergenic 30.0%
AACTACTGTTATTAGGGTTA+AGG + chr8_2:53140563-53140582 Msa1254850:five_prime_UTR 30.0%
!! AAACTCATGTTAAAAGCACT+TGG + chr8_2:53141069-53141088 Msa1254850:CDS 30.0%
!! AACTCATGTTAAAAGCACTT+GGG + chr8_2:53141070-53141089 Msa1254850:CDS 30.0%
CTTAACCATAACACTGAAAA+GGG - chr8_2:53141344-53141363 None:intergenic 30.0%
TCTTAACCATAACACTGAAA+AGG - chr8_2:53141345-53141364 None:intergenic 30.0%
! AAGCTGATGGATATGAAATA+TGG - chr8_2:53141536-53141555 None:intergenic 30.0%
AGGAAAAACAGTTTCTTTCT+TGG - chr8_2:53141648-53141667 None:intergenic 30.0%
TGCATTCTGTTGTATTGTTA+AGG + chr8_2:53141817-53141836 Msa1254850:intron 30.0%
TGATAGGTATGCATATCAAA+CGG + chr8_2:53141992-53142011 Msa1254850:CDS 30.0%
TGATCCTTCTTCATTGTATT+CGG + chr8_2:53142214-53142233 Msa1254850:CDS 30.0%
AGAAGAAAGAGGAGTTATTA+TGG + chr8_2:53142310-53142329 Msa1254850:three_prime_UTR 30.0%
GAAGAAAGAGGAGTTATTAT+GGG + chr8_2:53142311-53142330 Msa1254850:three_prime_UTR 30.0%
AAGAAAGAGGAGTTATTATG+GGG + chr8_2:53142312-53142331 Msa1254850:three_prime_UTR 30.0%
TCAAATTTACCTCTACTACT+TGG - chr8_2:53142426-53142445 None:intergenic 30.0%
!! TACTCTATAATGGACTTCTA+TGG + chr8_2:53142597-53142616 Msa1254850:three_prime_UTR 30.0%
CAAAAACAAACGTTGAAGGA+AGG + chr8_2:53140152-53140171 Msa1254850:five_prime_UTR 35.0%
! TATGTCTCCTTGTTATGGTT+GGG - chr8_2:53140336-53140355 None:intergenic 35.0%
! ATATGTCTCCTTGTTATGGT+TGG - chr8_2:53140337-53140356 None:intergenic 35.0%
TTCTTCTTCAAAGAAATGGC+TGG + chr8_2:53140591-53140610 Msa1254850:exon 35.0%
TACCAAAATAGTGTCTCTTG+TGG - chr8_2:53140687-53140706 None:intergenic 35.0%
GATGTTTCATCTTACAACAG+AGG + chr8_2:53140717-53140736 Msa1254850:CDS 35.0%
ATAACTGTTGTTGACGTTCA+CGG - chr8_2:53141112-53141131 None:intergenic 35.0%
! TCAACTCGTAATTTTCCTTC+AGG + chr8_2:53141194-53141213 Msa1254850:CDS 35.0%
TACTGTGACATAAGGTGTAA+GGG + chr8_2:53141281-53141300 Msa1254850:intron 35.0%
! ATTGACCCTTTTCAGTGTTA+TGG + chr8_2:53141336-53141355 Msa1254850:intron 35.0%
!!! GAGAGAATCTTTTGGTTTAG+AGG + chr8_2:53141400-53141419 Msa1254850:intron 35.0%
ATAAACACACGCAATCGATA+CGG - chr8_2:53141493-53141512 None:intergenic 35.0%
! CTGATGGATATGAAATATGG+TGG - chr8_2:53141533-53141552 None:intergenic 35.0%
AACAGTTTCTTTCTTGGTCA+GGG - chr8_2:53141642-53141661 None:intergenic 35.0%
AAACAGTTTCTTTCTTGGTC+AGG - chr8_2:53141643-53141662 None:intergenic 35.0%
!! CAATTGATGATGCAGATGAT+AGG + chr8_2:53141976-53141995 Msa1254850:CDS 35.0%
TATCAAACGGCTGTTAACAT+TGG + chr8_2:53142005-53142024 Msa1254850:CDS 35.0%
ATTGGAGGACATTTGTTCAA+AGG + chr8_2:53142023-53142042 Msa1254850:CDS 35.0%
CTATTGTTGGTACTACTTTC+TGG - chr8_2:53142068-53142087 None:intergenic 35.0%
TCCGATTAATGCCTTTATGA+CGG + chr8_2:53142238-53142257 Msa1254850:CDS 35.0%
AACAAACGTTGAAGGAAGGA+AGG + chr8_2:53140156-53140175 Msa1254850:five_prime_UTR 40.0%
GGACAGAAAACAGAAACAGA+CGG - chr8_2:53140266-53140285 None:intergenic 40.0%
! CTCTTTTCCCAACCATAACA+AGG + chr8_2:53140326-53140345 Msa1254850:five_prime_UTR 40.0%
GACGCACACAAAAGAAAACA+CGG + chr8_2:53140400-53140419 Msa1254850:five_prime_UTR 40.0%
!! ATGGCTGGTTTGTTCTCATT+AGG + chr8_2:53140606-53140625 Msa1254850:CDS 40.0%
GTTGTTGTGATTCTTCTCCT+TGG - chr8_2:53140650-53140669 None:intergenic 40.0%
GGAGAAGAATCACAACAACA+AGG + chr8_2:53140651-53140670 Msa1254850:CDS 40.0%
CAAAATAGTGTCTCTTGTGG+TGG - chr8_2:53140684-53140703 None:intergenic 40.0%
! CACCACAAGAGACACTATTT+TGG + chr8_2:53140682-53140701 Msa1254850:CDS 40.0%
!! CATCTTGTTGTTGTTGTTGC+TGG - chr8_2:53140794-53140813 None:intergenic 40.0%
AAGATGATATGCATGCTGCA+CGG + chr8_2:53140808-53140827 Msa1254850:CDS 40.0%
CATGTGAGGACAATCTTTCT+TGG - chr8_2:53141009-53141028 None:intergenic 40.0%
CAAGTTCTACATCTCATGTG+AGG - chr8_2:53141023-53141042 None:intergenic 40.0%
!! AGAACTTGTTGTAAGAGTCG+TGG + chr8_2:53141035-53141054 Msa1254850:CDS 40.0%
ATTTCAAAACGCCCTAGAGA+TGG + chr8_2:53141155-53141174 Msa1254850:CDS 40.0%
GAGCATTTGAACCATCTCTA+GGG - chr8_2:53141169-53141188 None:intergenic 40.0%
! AGAGCATTTGAACCATCTCT+AGG - chr8_2:53141170-53141189 None:intergenic 40.0%
TGGACTTGTACTGTGACATA+AGG + chr8_2:53141273-53141292 Msa1254850:intron 40.0%
GTACTGTGACATAAGGTGTA+AGG + chr8_2:53141280-53141299 Msa1254850:intron 40.0%
ACACTGAAAAGGGTCAATCA+CGG - chr8_2:53141334-53141353 None:intergenic 40.0%
GTTATGGTTAAGAGAGTGAG+TGG + chr8_2:53141352-53141371 Msa1254850:intron 40.0%
GAGAGTGAGTGGTGAAATAA+AGG + chr8_2:53141363-53141382 Msa1254850:intron 40.0%
GATTGAGGTGAGAATATGTG+TGG - chr8_2:53141706-53141725 None:intergenic 40.0%
ATGATGGTAGCTAGTGATTG+AGG - chr8_2:53141721-53141740 None:intergenic 40.0%
AACAACAGTCGTATCAGAGT+CGG - chr8_2:53142174-53142193 None:intergenic 40.0%
!! GCCGTCATAAAGGCATTAAT+CGG - chr8_2:53142242-53142261 None:intergenic 40.0%
AGGAGTTATTATGGGGATCA+TGG + chr8_2:53142319-53142338 Msa1254850:three_prime_UTR 40.0%
! TGACCATGACATTGTTGTTG+TGG + chr8_2:53142400-53142419 Msa1254850:three_prime_UTR 40.0%
AACGTTGAAGGAAGGAAGGA+AGG + chr8_2:53140160-53140179 Msa1254850:five_prime_UTR 45.0%
TTGAAGGAAGGAAGGAAGGA+AGG + chr8_2:53140164-53140183 Msa1254850:five_prime_UTR 45.0%
!! GCTGGTTTGTTCTCATTAGG+AGG + chr8_2:53140609-53140628 Msa1254850:CDS 45.0%
! GGTTTGTTCTCATTAGGAGG+TGG + chr8_2:53140612-53140631 Msa1254850:CDS 45.0%
TTGTTCTCATTAGGAGGTGG+TGG + chr8_2:53140615-53140634 Msa1254850:CDS 45.0%
!! GTTGTTGGTGCTGTTGTTGT+TGG - chr8_2:53140767-53140786 None:intergenic 45.0%
!! CTTGTTGTTGTTGTTGCTGG+TGG - chr8_2:53140791-53140810 None:intergenic 45.0%
!! TTTAGGTGTTGGACCAAGCA+GGG + chr8_2:53140869-53140888 Msa1254850:CDS 45.0%
TCATCTGATGAAACCCTGCT+TGG - chr8_2:53140885-53140904 None:intergenic 45.0%
ACATCAATCGTCGTCGAGAT+CGG + chr8_2:53140911-53140930 Msa1254850:CDS 45.0%
CAGCGTTTACTATGGCTATG+AGG + chr8_2:53140934-53140953 Msa1254850:CDS 45.0%
AGGTCTTCTTCTTCAGCTGA+AGG + chr8_2:53140954-53140973 Msa1254850:CDS 45.0%
GGTCTTCTTCTTCAGCTGAA+GGG + chr8_2:53140955-53140974 Msa1254850:CDS 45.0%
GGGATAAGTTGCCAAGATTG+TGG + chr8_2:53140975-53140994 Msa1254850:CDS 45.0%
!! TGGCTTGGTTACCACAATCT+TGG - chr8_2:53140989-53141008 None:intergenic 45.0%
GAGGACAATCTTTCTTGGCT+TGG - chr8_2:53141004-53141023 None:intergenic 45.0%
GGAATGTGCTAAGAAAGAGG+AGG - chr8_2:53141668-53141687 None:intergenic 45.0%
GAGGGAATGTGCTAAGAAAG+AGG - chr8_2:53141671-53141690 None:intergenic 45.0%
GTGTGGATGTGAAAGAGAGA+GGG - chr8_2:53141689-53141708 None:intergenic 45.0%
TGTGTGGATGTGAAAGAGAG+AGG - chr8_2:53141690-53141709 None:intergenic 45.0%
! GGAAGCAAATTTTCCTGCTG+TGG + chr8_2:53141914-53141933 Msa1254850:CDS 45.0%
CAAACGGCTGTTAACATTGG+AGG + chr8_2:53142008-53142027 Msa1254850:CDS 45.0%
TACATGATTGGGGAGACTTC+CGG + chr8_2:53142107-53142126 Msa1254850:CDS 45.0%
GAGTCGGTGATTAGGTTCAA+CGG - chr8_2:53142158-53142177 None:intergenic 45.0%
TCGTATCAGAGTCGGTGATT+AGG - chr8_2:53142166-53142185 None:intergenic 45.0%
GGAGCCGAATACAATGAAGA+AGG - chr8_2:53142221-53142240 None:intergenic 45.0%
AATGCCTTTATGACGGCTAG+TGG + chr8_2:53142245-53142264 Msa1254850:CDS 45.0%
!! GTTTTCAAGATCTTGGGCGA+GGG - chr8_2:53142283-53142302 None:intergenic 45.0%
!! TGTTTTCAAGATCTTGGGCG+AGG - chr8_2:53142284-53142303 None:intergenic 45.0%
TGGCCACAACAACAATGTCA+TGG - chr8_2:53142406-53142425 None:intergenic 45.0%
TTGTTGTGGCCAAGTAGTAG+AGG + chr8_2:53142414-53142433 Msa1254850:three_prime_UTR 45.0%
!!! TTTGTTTTATTAAAAAATAA+AGG + chr8_2:53141885-53141904 Msa1254850:intron 5.0%
!!! TTGTTTTATTAAAAAATAAA+GGG + chr8_2:53141886-53141905 Msa1254850:intron 5.0%
AAGCTATAGCTGCTGCTAGC+TGG + chr8_2:53140187-53140206 Msa1254850:five_prime_UTR 50.0%
AGCTATAGCTGCTGCTAGCT+GGG + chr8_2:53140188-53140207 Msa1254850:five_prime_UTR 50.0%
!! TCATTAGGAGGTGGTGGAAG+AGG + chr8_2:53140621-53140640 Msa1254850:CDS 50.0%
!! GGTGCTGTTGTTGTTGGTGT+TGG - chr8_2:53140761-53140780 None:intergenic 50.0%
!! GTTGTTGCTGGTGGTGTTGT+TGG - chr8_2:53140782-53140801 None:intergenic 50.0%
TAGAGATCTCGCGGGAAGAA+CGG - chr8_2:53140834-53140853 None:intergenic 50.0%
TTCTTCCCGCGAGATCTCTA+CGG + chr8_2:53140834-53140853 Msa1254850:CDS 50.0%
! TACGGTCCAGGAGTTGGTTT+AGG + chr8_2:53140852-53140871 Msa1254850:CDS 50.0%
CCAACACCTAAACCAACTCC+TGG - chr8_2:53140861-53140880 None:intergenic 50.0%
! CCAGGAGTTGGTTTAGGTGT+TGG + chr8_2:53140858-53140877 Msa1254850:CDS 50.0%
!! GTTTAGGTGTTGGACCAAGC+AGG + chr8_2:53140868-53140887 Msa1254850:CDS 50.0%
GAGATCGGCAGCGTTTACTA+TGG + chr8_2:53140926-53140945 Msa1254850:CDS 50.0%
ACACACGCAATCGATACGGT+CGG - chr8_2:53141489-53141508 None:intergenic 50.0%
AGCGGAGTTCAGGTGTGTAA+GGG + chr8_2:53141947-53141966 Msa1254850:CDS 50.0%
TACGACTGTTGTTGCCTCCT+CGG + chr8_2:53142181-53142200 Msa1254850:CDS 50.0%
CGTACCACTAGCCGTCATAA+AGG - chr8_2:53142252-53142271 None:intergenic 50.0%
CAGACGGCTAAACAGTGTCG+TGG - chr8_2:53140250-53140269 None:intergenic 55.0%
!! GGTGGTGGAAGAGGAAACCA+AGG + chr8_2:53140630-53140649 Msa1254850:CDS 55.0%
GATCTCTACGGTCCAGGAGT+TGG + chr8_2:53140846-53140865 Msa1254850:CDS 55.0%
TGCTGTGGTAAGCTCACCAG+CGG + chr8_2:53141929-53141948 Msa1254850:CDS 55.0%
TAAGCTCACCAGCGGAGTTC+AGG + chr8_2:53141937-53141956 Msa1254850:CDS 55.0%
CTTACACACCTGAACTCCGC+TGG - chr8_2:53141948-53141967 None:intergenic 55.0%
CAGCGGAGTTCAGGTGTGTA+AGG + chr8_2:53141946-53141965 Msa1254850:CDS 55.0%
ATGATTGGGGAGACTTCCGG+TGG + chr8_2:53142110-53142129 Msa1254850:CDS 55.0%
ACGACTGTTGTTGCCTCCTC+GGG + chr8_2:53142182-53142201 Msa1254850:CDS 55.0%
CTGGACCGTAGAGATCTCGC+GGG - chr8_2:53140842-53140861 None:intergenic 60.0%
CCTGGACCGTAGAGATCTCG+CGG - chr8_2:53140843-53140862 None:intergenic 60.0%
CGTTCACGGCGTCTAGAAGC+AGG - chr8_2:53141098-53141117 None:intergenic 60.0%
GCTGGTGAGCTTACCACAGC+AGG - chr8_2:53141930-53141949 None:intergenic 60.0%
ATTGGGGAGACTTCCGGTGG+AGG + chr8_2:53142113-53142132 Msa1254850:CDS 60.0%
ACTTCCGGTGGAGGTGGTGT+TGG + chr8_2:53142122-53142141 Msa1254850:CDS 60.0%
AACGCCAACACCACCTCCAC+CGG - chr8_2:53142129-53142148 None:intergenic 60.0%
ATCAACAAGTGCGCCCGAGG+AGG - chr8_2:53142198-53142217 None:intergenic 60.0%
AGGATCAACAAGTGCGCCCG+AGG - chr8_2:53142201-53142220 None:intergenic 60.0%
CCGCGAGATCTCTACGGTCC+AGG + chr8_2:53140840-53140859 Msa1254850:CDS 65.0%
GGGGAGACTTCCGGTGGAGG+TGG + chr8_2:53142116-53142135 Msa1254850:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 53140046 53142644 53140046 ID=Msa1254850;Name=Msa1254850
chr8_2 mRNA 53140046 53142644 53140046 ID=Msa1254850-mRNA-1;Parent=Msa1254850;Name=Msa1254850-mRNA-1;_AED=0.00;_eAED=0.00;_QI=560|1|1|1|1|1|2|346|333
chr8_2 exon 53140046 53141215 53140046 ID=Msa1254850-mRNA-1:exon:16384;Parent=Msa1254850-mRNA-1
chr8_2 exon 53141907 53142644 53141907 ID=Msa1254850-mRNA-1:exon:16385;Parent=Msa1254850-mRNA-1
chr8_2 five_prime_UTR 53140046 53140605 53140046 ID=Msa1254850-mRNA-1:five_prime_utr;Parent=Msa1254850-mRNA-1
chr8_2 CDS 53140606 53141215 53140606 ID=Msa1254850-mRNA-1:cds;Parent=Msa1254850-mRNA-1
chr8_2 CDS 53141907 53142298 53141907 ID=Msa1254850-mRNA-1:cds;Parent=Msa1254850-mRNA-1
chr8_2 three_prime_UTR 53142299 53142644 53142299 ID=Msa1254850-mRNA-1:three_prime_utr;Parent=Msa1254850-mRNA-1
Gene Sequence

>Msa1254850

ATGGCTGGTTTGTTCTCATTAGGAGGTGGTGGAAGAGGAAACCAAGGAGAAGAATCACAACAACAAGGTCATATTCCACCACAAGAGACACTATTTTGGTACAAAAATGATGATGTTTCATCTTACAACAGAGGTAATTTAGAATTATGGAACCAACACCAACAACAACAGCACCAACAACACCACCAGCAACAACAACAACAAGATGATATGCATGCTGCACGGCCGTTCTTCCCGCGAGATCTCTACGGTCCAGGAGTTGGTTTAGGTGTTGGACCAAGCAGGGTTTCATCAGATGATCATGAACATCAATCGTCGTCGAGATCGGCAGCGTTTACTATGGCTATGAGGTCTTCTTCTTCAGCTGAAGGGATAAGTTGCCAAGATTGTGGTAACCAAGCCAAGAAAGATTGTCCTCACATGAGATGTAGAACTTGTTGTAAGAGTCGTGGCTTTCAGTGTCAAACTCATGTTAAAAGCACTTGGGTTCCTGCTTCTAGACGCCGTGAACGTCAACAACAGTTATCTTCTTCTCCTTTACAAAGAGATATTTCAAAACGCCCTAGAGATGGTTCAAATGCTCTTGTTTCAACTCGTAATTTTCCTTCAGGGTTAGAGGAAGCAAATTTTCCTGCTGTGGTAAGCTCACCAGCGGAGTTCAGGTGTGTAAGGGTTAGTTCAATTGATGATGCAGATGATAGGTATGCATATCAAACGGCTGTTAACATTGGAGGACATTTGTTCAAAGGAATTCTCTATGATTTTGGTCCAGAAAGTAGTACCAACAATAGTAATTATAATAATAGTAATTACATGATTGGGGAGACTTCCGGTGGAGGTGGTGTTGGCGTTGCTCAACCGTTGAACCTAATCACCGACTCTGATACGACTGTTGTTGCCTCCTCGGGCGCACTTGTTGATCCTTCTTCATTGTATTCGGCTCCGATTAATGCCTTTATGACGGCTAGTGGTACGCAATTCTTCCCTCGCCCAAGATCTTGA
Protein sequence

>Msa1254850

MAGLFSLGGGGRGNQGEESQQQGHIPPQETLFWYKNDDVSSYNRGNLELWNQHQQQQHQQHHQQQQQQDDMHAARPFFPRDLYGPGVGLGVGPSRVSSDDHEHQSSSRSAAFTMAMRSSSSAEGISCQDCGNQAKKDCPHMRCRTCCKSRGFQCQTHVKSTWVPASRRRERQQQLSSSPLQRDISKRPRDGSNALVSTRNFPSGLEEANFPAVVSSPAEFRCVRVSSIDDADDRYAYQTAVNIGGHLFKGILYDFGPESSTNNSNYNNSNYMIGETSGGGGVGVAQPLNLITDSDTTVVASSGALVDPSSLYSAPINAFMTASGTQFFPRPRS*