AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1255350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1255350 MtrunA17_Chr8g0373541 97.323 523 8 2 1 521 1 519 0.0 1030
Msa1255350 MtrunA17_Chr2g0330171 75.120 209 36 1 4 196 1 209 1.16e-117 355
Msa1255350 MtrunA17_Chr8g0342421 76.077 209 35 2 3 196 17 225 4.37e-117 355
Msa1255350 MtrunA17_Chr4g0040571 47.805 410 169 8 29 413 35 424 6.04e-117 354
Msa1255350 MtrunA17_Chr1g0191991 88.372 172 19 1 31 202 61 231 1.04e-115 352
Msa1255350 MtrunA17_Chr1g0181381 84.091 176 22 2 26 195 26 201 2.62e-111 339
Msa1255350 MtrunA17_Chr4g0029521 76.842 190 39 2 11 195 12 201 3.90e-111 339
Msa1255350 MtrunA17_Chr1g0185121 47.549 408 120 10 4 397 6 333 4.41e-110 335
Msa1255350 MtrunA17_Chr7g0263941 79.032 186 34 1 15 195 11 196 1.29e-109 332
Msa1255350 MtrunA17_Chr1g0199101 85.185 162 24 0 31 192 45 206 7.58e-109 334
Msa1255350 MtrunA17_Chr1g0163001 84.242 165 26 0 31 195 41 205 1.19e-107 331
Msa1255350 MtrunA17_Chr1g0151351 85.366 164 23 1 32 194 40 203 3.14e-107 327
Msa1255350 MtrunA17_Chr1g0162971 68.137 204 61 1 33 236 41 240 3.55e-106 324
Msa1255350 MtrunA17_Chr1g0210821 83.439 157 26 0 36 192 47 203 3.55e-104 323
Msa1255350 MtrunA17_Chr7g0268961 86.928 153 20 0 43 195 32 184 8.68e-104 320
Msa1255350 MtrunA17_Chr2g0326381 77.576 165 36 1 32 195 31 195 7.85e-100 307
Msa1255350 MtrunA17_Chr2g0324991 90.076 131 13 0 44 174 3 133 3.63e-88 267
Msa1255350 MtrunA17_Chr6g0479921 63.253 166 60 1 33 197 29 194 4.88e-75 244
Msa1255350 MtrunA17_Chr8g0375521 46.923 260 104 6 4 257 2 233 2.92e-73 238
Msa1255350 MtrunA17_Chr3g0123941 32.642 193 118 4 38 218 113 305 3.23e-24 103
Msa1255350 MtrunA17_Chr2g0320341 34.839 155 87 3 42 182 224 378 2.36e-23 102
Msa1255350 MtrunA17_Chr1g0200421 34.437 151 87 3 47 185 236 386 3.78e-23 102
Msa1255350 MtrunA17_Chr5g0422571 32.243 214 130 7 9 212 65 273 2.03e-22 97.4
Msa1255350 MtrunA17_Chr8g0382121 35.762 151 86 5 43 182 106 256 3.22e-21 94.4
Msa1255350 MtrunA17_Chr7g0258011 34.615 156 82 7 56 193 183 336 4.32e-19 89.0
Msa1255350 MtrunA17_Chr8g0352011 35.256 156 81 6 56 193 173 326 1.07e-18 87.4
Msa1255350 MtrunA17_Chr4g0053341 33.113 151 91 4 44 185 69 218 3.88e-18 83.6
Msa1255350 MtrunA17_Chr1g0198651 32.680 153 90 4 56 196 109 260 7.43e-18 84.3
Msa1255350 MtrunA17_Chr1g0173841 34.532 139 81 4 56 185 222 359 8.27e-18 85.5
Msa1255350 MtrunA17_Chr4g0026521 35.252 139 80 5 56 185 125 262 1.29e-17 83.6
Msa1255350 MtrunA17_Chr2g0328921 32.927 164 92 6 31 185 128 282 3.04e-17 82.8
Msa1255350 MtrunA17_Chr2g0321291 33.333 168 98 6 30 185 112 277 4.67e-17 82.0
Msa1255350 MtrunA17_Chr3g0141431 42.149 121 57 4 375 495 300 407 4.57e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1255350 AT3G50700.1 56.238 521 151 14 2 519 3 449 1.22e-179 513
Msa1255350 AT5G66730.1 58.071 539 166 15 2 521 3 500 1.83e-178 511
Msa1255350 AT1G03840.1 86.228 167 23 0 29 195 37 203 3.41e-111 340
Msa1255350 AT5G44160.1 78.351 194 40 1 4 195 6 199 6.99e-111 338
Msa1255350 AT1G55110.3 70.507 217 49 5 3 204 20 236 3.11e-108 330
Msa1255350 AT1G55110.1 70.507 217 49 5 3 204 20 236 3.11e-108 330
Msa1255350 AT1G55110.2 70.507 217 49 5 3 204 20 236 3.11e-108 330
Msa1255350 AT1G03840.2 85.030 167 23 1 29 195 37 201 8.13e-108 331
Msa1255350 AT3G13810.4 70.233 215 42 2 3 195 19 233 2.35e-106 327
Msa1255350 AT3G13810.1 70.233 215 42 2 3 195 19 233 2.35e-106 327
Msa1255350 AT3G13810.5 70.233 215 42 2 3 195 14 228 2.57e-106 327
Msa1255350 AT3G13810.6 70.561 214 41 2 4 195 1 214 2.88e-106 327
Msa1255350 AT2G02080.1 78.916 166 35 0 31 196 52 217 4.15e-106 327
Msa1255350 AT2G02080.4 78.916 166 35 0 31 196 52 217 4.15e-106 327
Msa1255350 AT2G02080.5 78.916 166 35 0 31 196 52 217 4.15e-106 327
Msa1255350 AT2G02080.3 78.916 166 35 0 31 196 52 217 4.15e-106 327
Msa1255350 AT4G02670.2 73.737 198 42 3 8 195 21 218 1.53e-105 322
Msa1255350 AT4G02670.1 73.737 198 42 3 8 195 19 216 1.60e-105 322
Msa1255350 AT1G14580.1 76.608 171 40 0 26 196 46 216 4.97e-105 323
Msa1255350 AT1G14580.2 76.608 171 40 0 26 196 46 216 4.97e-105 323
Msa1255350 AT5G03150.1 74.872 195 41 3 33 219 53 247 8.75e-105 323
Msa1255350 AT1G14580.3 76.608 171 40 0 26 196 60 230 9.19e-105 322
Msa1255350 AT3G45260.2 81.098 164 30 1 33 195 39 202 5.19e-104 319
Msa1255350 AT3G45260.1 81.098 164 30 1 33 195 39 202 5.19e-104 319
Msa1255350 AT5G44160.2 88.235 153 18 0 43 195 8 160 7.18e-103 316
Msa1255350 AT2G02070.2 80.368 163 32 0 33 195 52 214 1.12e-102 321
Msa1255350 AT2G02070.1 80.368 163 32 0 33 195 52 214 1.12e-102 321
Msa1255350 AT5G60470.2 77.246 167 37 1 30 195 41 207 2.45e-100 310
Msa1255350 AT5G60470.3 77.246 167 37 1 30 195 37 203 4.15e-100 310
Msa1255350 AT3G13810.3 87.582 153 18 1 44 195 68 220 8.93e-99 308
Msa1255350 AT3G13810.2 87.582 153 18 1 44 195 82 234 1.03e-98 308
Msa1255350 AT5G60470.1 70.520 173 50 1 24 195 26 198 3.06e-94 294
Msa1255350 AT2G02080.6 80.714 140 27 0 57 196 1 140 1.51e-91 287
Msa1255350 AT2G02080.2 80.714 140 27 0 57 196 1 140 1.51e-91 287
Msa1255350 AT1G25250.3 68.153 157 47 2 33 186 33 189 3.42e-75 243
Msa1255350 AT1G68130.1 67.308 156 49 2 33 186 41 196 2.81e-74 241
Msa1255350 AT2G01940.1 65.190 158 53 2 33 188 44 201 1.92e-73 240
Msa1255350 AT1G25250.1 68.456 149 44 2 41 186 18 166 1.06e-71 233
Msa1255350 AT2G01940.3 63.522 159 55 3 33 188 44 202 3.42e-69 229
Msa1255350 AT1G25250.2 67.568 111 33 2 79 186 1 111 1.15e-47 168
Msa1255350 AT1G68130.2 65.455 110 36 2 79 186 1 110 1.73e-46 165
Msa1255350 AT2G01940.2 64.286 112 38 2 79 188 1 112 2.37e-46 166
Msa1255350 AT5G22890.1 34.000 150 85 3 47 182 202 351 3.97e-23 101
Msa1255350 AT1G34370.3 33.962 159 91 3 41 185 13 171 5.56e-23 99.4
Msa1255350 AT1G34370.2 33.962 159 91 3 41 185 223 381 9.39e-23 102
Msa1255350 AT1G34370.1 33.962 159 91 3 41 185 223 381 9.39e-23 102
Msa1255350 AT1G13290.1 36.986 146 80 6 56 190 95 239 3.91e-19 88.6
Msa1255350 AT1G34790.1 34.667 150 88 4 45 185 128 276 2.24e-17 83.6
Msa1255350 AT1G51220.1 33.094 139 83 4 56 185 172 309 3.11e-17 83.6
Msa1255350 AT3G57670.1 30.588 170 100 5 56 208 211 379 3.60e-17 84.0
Msa1255350 AT3G20880.1 33.813 139 82 4 56 185 250 387 6.24e-17 83.6
Msa1255350 AT1G08290.1 36.296 135 75 4 61 185 179 312 9.62e-17 82.0

Find 160 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTTGTGACGGCGATTC+TGG 0.135600 8_2:+53859014 Msa1255350:CDS
ATATGGTTGTCTTAAGATTT+AGG 0.166620 8_2:+53859573 Msa1255350:three_prime_UTR
CTTAAATATGGTCTTTCTTC+TGG 0.195481 8_2:-53859471 None:intergenic
CAGAAGAAAGACCATATTTA+AGG 0.205433 8_2:+53859472 Msa1255350:three_prime_UTR
ACACAGGCTGTGAGAAATTA+AGG 0.244185 8_2:+53859542 Msa1255350:three_prime_UTR
CATGATCCTTCTAGAGCTTT+AGG 0.245902 8_2:+53857323 Msa1255350:CDS
GAAGTTCATGATAGATATTT+TGG 0.250600 8_2:+53859246 Msa1255350:exon
GCCTCTCTGCTTCGCGGGCT+TGG 0.251735 8_2:+53858876 Msa1255350:CDS
GTCTTTCTTCTGGCATTATA+AGG 0.267065 8_2:-53859461 None:intergenic
TAATTTGTACCTGGCATTCC+TGG 0.277575 8_2:-53857014 None:intergenic
CAACTACTAGTGTTATTATC+AGG 0.290858 8_2:+53859278 Msa1255350:three_prime_UTR
AGAGCTTTAGGTGATTTAAC+TGG 0.295896 8_2:+53857335 Msa1255350:CDS
AAAGATCAAGTAAAGAAATT+AGG 0.296295 8_2:+53857261 Msa1255350:CDS
GACAATGTTTCCACAGCTTC+AGG 0.299307 8_2:+53856912 Msa1255350:CDS
GGACTAAAGGAATTGATGTT+AGG 0.301999 8_2:+53859035 Msa1255350:CDS
TATGGTTGTCTTAAGATTTA+GGG 0.302414 8_2:+53859574 Msa1255350:three_prime_UTR
CGTAGCTGTAGGTATAAATT+AGG 0.307449 8_2:+53859686 Msa1255350:three_prime_UTR
CTAGGATTGGGGATGGCTGC+TGG 0.321231 8_2:+53859101 Msa1255350:CDS
ACTCTTGATTTCCTAGGATT+GGG 0.333570 8_2:+53859089 Msa1255350:CDS
TATTCCTCAGATGGATGATT+TGG 0.337901 8_2:+53859661 Msa1255350:three_prime_UTR
TACATCCCATGGACCTAATT+AGG 0.343619 8_2:-53858653 None:intergenic
ATGGCTGCTGGTGGCAGTGC+CGG 0.355268 8_2:+53859113 Msa1255350:CDS
AAGGTACAATGTGCTTGTTT+TGG 0.357051 8_2:+53859516 Msa1255350:three_prime_UTR
TTGATTTGTTGCAGATTTGC+CGG 0.357508 8_2:+53858428 Msa1255350:intron
AACTCTTGATTTCCTAGGAT+TGG 0.365068 8_2:+53859088 Msa1255350:CDS
AGAGCAATCACCTCTGCTTC+TGG 0.372321 8_2:-53857116 None:intergenic
CAATGTGCAATATGCAACTA+TGG 0.374452 8_2:+53859401 Msa1255350:three_prime_UTR
GAGTTCTCTGGCAAAGATAT+TGG 0.378609 8_2:+53859221 Msa1255350:CDS
TGCAACAGGTAATGTGCAAA+TGG 0.385687 8_2:+53859429 Msa1255350:three_prime_UTR
CAAGTAAAGAAATTAGGAAA+AGG 0.395504 8_2:+53857267 Msa1255350:CDS
AAACATTTGTTGTCTATGAA+TGG 0.395676 8_2:+53859324 Msa1255350:three_prime_UTR
TTCCACGTTTGTCCATTGTT+TGG 0.395965 8_2:+53859764 Msa1255350:three_prime_UTR
ATAGCTATAGTTTATAGCTT+AGG 0.399501 8_2:+53856846 Msa1255350:five_prime_UTR
TCTTCTGCTTCCACTTCATC+TGG 0.404493 8_2:+53858909 Msa1255350:CDS
CAGACAACTCTTGATTTCCT+AGG 0.415465 8_2:+53859083 Msa1255350:CDS
GATAGCCTGCATTGGGGTCT+CGG 0.418830 8_2:+53858939 Msa1255350:CDS
TTAGGGACTCCGTCAATGTT+TGG 0.420641 8_2:+53859053 Msa1255350:CDS
TGGAATTATTGTCACATTTG+TGG 0.424814 8_2:+53859739 Msa1255350:three_prime_UTR
AATATGCTGTTCAATCTGAT+TGG 0.427517 8_2:+53857423 Msa1255350:CDS
GAAAATTGATGATCCATGAT+TGG 0.434295 8_2:-53858742 None:intergenic
ACCGCAGCAGCCGCATCCTT+CGG 0.436819 8_2:+53859191 Msa1255350:CDS
TTTGTAGCTAACAACGTTGT+TGG 0.437085 8_2:-53857143 None:intergenic
AAGCACATTGTACCTTGTTA+AGG 0.439079 8_2:-53859509 None:intergenic
AAAGCTCACTCTAAAGTTTG+TGG 0.439997 8_2:+53857446 Msa1255350:CDS
CAAACGCCTCTCTGCTTCGC+GGG 0.440537 8_2:+53858871 Msa1255350:CDS
AAGTAAAGAAATTAGGAAAA+GGG 0.440652 8_2:+53857268 Msa1255350:CDS
GCAACAGGTAATGTGCAAAT+GGG 0.445106 8_2:+53859430 Msa1255350:three_prime_UTR
CTTTGCCTCTCGACCAATCA+TGG 0.452274 8_2:+53858729 Msa1255350:CDS
TGTGGCTCCTCTATGATTTG+TGG 0.452926 8_2:-53858459 None:intergenic
GTTTCAGCAGGACTCGGACT+AGG 0.454067 8_2:+53858990 Msa1255350:CDS
GCATTGGGGTCTCGGACAAA+TGG 0.454354 8_2:+53858947 Msa1255350:CDS
AATTGGCGGTGGCAGTGGTC+TGG 0.454398 8_2:+53859163 Msa1255350:CDS
TCAACAAGATAGCCTGCATT+GGG 0.455422 8_2:+53858932 Msa1255350:CDS
GGTTCTAGTTTAGTTTCAGC+AGG 0.456585 8_2:+53858978 Msa1255350:CDS
CAACTATGGATCAATGCAAC+AGG 0.464041 8_2:+53859415 Msa1255350:three_prime_UTR
GATCTTTGTCTCAGTTTCCA+TGG 0.464582 8_2:-53857245 None:intergenic
GCTGGTGGCAGTGCCGGTGG+AGG 0.466700 8_2:+53859119 Msa1255350:CDS
TCTGCACTTATCACATCAAT+TGG 0.468012 8_2:+53859146 Msa1255350:CDS
TAGTGGTGTGTCCTCTGCTT+TGG 0.468292 8_2:+53857820 Msa1255350:CDS
AAATCACCTAAAGCTCTAGA+AGG 0.470379 8_2:-53857329 None:intergenic
ACATCAATTGGCGGTGGCAG+TGG 0.474797 8_2:+53859158 Msa1255350:CDS
TTCGAAGCCGGTGTCGATGC+CGG 0.475053 8_2:+53857754 Msa1255350:CDS
GCTGATGTGTTGGCACGTAT+TGG 0.475745 8_2:-53858788 None:intergenic
GGCTGTGCTACCACCAGCAC+TGG 0.475967 8_2:+53858552 Msa1255350:CDS
GCAGCCGCATCCTTCGGTAA+TGG 0.482125 8_2:+53859197 Msa1255350:CDS
AAATCAAGAGTTGTCTGCTT+AGG 0.482911 8_2:-53859077 None:intergenic
AGTTTAGTTTCAGCAGGACT+CGG 0.486435 8_2:+53858984 Msa1255350:CDS
ACAAACGCCTCTCTGCTTCG+CGG 0.487979 8_2:+53858870 Msa1255350:CDS
GACTAAAGGAATTGATGTTA+GGG 0.488496 8_2:+53859036 Msa1255350:CDS
TTCGGTAATGGAGAGTTCTC+TGG 0.494928 8_2:+53859209 Msa1255350:CDS
ATCGCGGGCGGCTGATGTGT+TGG 0.496253 8_2:-53858798 None:intergenic
TGATGAACACAAGTAGGTTC+AGG 0.499151 8_2:-53857302 None:intergenic
AGAAATCTCCCAGGAATGCC+AGG 0.500143 8_2:+53857005 Msa1255350:CDS
CAGCGACCTAATTAGGTCCA+TGG 0.501360 8_2:+53858647 Msa1255350:CDS
TTCTGCGAGTCTCCAACCTC+AGG 0.501471 8_2:+53858614 Msa1255350:CDS
GCTCGCAAACACGCCAGTGC+TGG 0.501531 8_2:-53858565 None:intergenic
GCTGCTGGTGGCAGTGCCGG+TGG 0.503660 8_2:+53859116 Msa1255350:CDS
CAAATCATGTATTCCTCAGA+TGG 0.506633 8_2:+53859652 Msa1255350:three_prime_UTR
CAGCAGCCATCCCCAATCCT+AGG 0.507214 8_2:-53859100 None:intergenic
TCGCTGAACGCCGGTGCCTG+AGG 0.511411 8_2:-53858630 None:intergenic
AAATTAAGGGTATGTGCATA+TGG 0.514034 8_2:+53859556 Msa1255350:three_prime_UTR
GTCAACAAGATAGCCTGCAT+TGG 0.514883 8_2:+53858931 Msa1255350:CDS
CATTGTCTAAATCAACCATT+GGG 0.515965 8_2:-53856896 None:intergenic
CACAGGCTGTGAGAAATTAA+GGG 0.516414 8_2:+53859543 Msa1255350:three_prime_UTR
ACATTGTCTAAATCAACCAT+TGG 0.516475 8_2:-53856897 None:intergenic
AATTTGTACCTGGCATTCCT+GGG 0.519747 8_2:-53857013 None:intergenic
TGAACGCCGGTGCCTGAGGT+TGG 0.520285 8_2:-53858626 None:intergenic
TAATTCAGAATCTGATTCAA+AGG 0.520820 8_2:+53857718 Msa1255350:CDS
GAAGCTAGCATCTCTTCCTC+TGG 0.524982 8_2:+53856936 Msa1255350:CDS
TGATTTCCTAGGATTGGGGA+TGG 0.525019 8_2:+53859094 Msa1255350:CDS
AGAGAGATCGCCTCGGACGA+TGG 0.526923 8_2:-53858708 None:intergenic
ACTACTCCTCAGTCAAATAG+TGG 0.528176 8_2:+53857803 Msa1255350:CDS
AATGCAGTGGCAGACATCGC+GGG 0.532433 8_2:-53858813 None:intergenic
GGCTCCTCTATGATTTGTGG+TGG 0.536477 8_2:-53858456 None:intergenic
CTCTTGATTTCCTAGGATTG+GGG 0.537741 8_2:+53859090 Msa1255350:CDS
AGTGGAGCATTTGAAGATCA+TGG 0.543917 8_2:+53859599 Msa1255350:three_prime_UTR
GAAAGACCATATTTAAGGAA+AGG 0.544279 8_2:+53859477 Msa1255350:three_prime_UTR
CTAATTTATACCTACAGCTA+CGG 0.546589 8_2:-53859685 None:intergenic
GAGCTTTAGGTGATTTAACT+GGG 0.546954 8_2:+53857336 Msa1255350:CDS
TTTGACCGGTGATTCGAAGC+CGG 0.547642 8_2:+53857742 Msa1255350:CDS
CGACACCGGCTTCGAATCAC+CGG 0.547911 8_2:-53857747 None:intergenic
TGCTTAGGACCAAACATTGA+CGG 0.548417 8_2:-53859062 None:intergenic
TGACGGCGATTCTGGACTAA+AGG 0.548568 8_2:+53859022 Msa1255350:CDS
CGCAAACACGCCAGTGCTGG+TGG 0.550873 8_2:-53858562 None:intergenic
ACCGAAGGATGCGGCTGCTG+CGG 0.555962 8_2:-53859192 None:intergenic
GTCTTAAGATTTAGGGACAG+TGG 0.559908 8_2:+53859581 Msa1255350:three_prime_UTR
TTTGTCCGAGACCCCAATGC+AGG 0.560302 8_2:-53858944 None:intergenic
GGATTGGGGATGGCTGCTGG+TGG 0.560903 8_2:+53859104 Msa1255350:CDS
AGAGAGTATAAATGTGATTG+TGG 0.563676 8_2:+53857473 Msa1255350:CDS
AACTACTAGTGTTATTATCA+GGG 0.565154 8_2:+53859279 Msa1255350:three_prime_UTR
GTGTGTGAAATATGTAACAA+AGG 0.568738 8_2:+53857173 Msa1255350:CDS
CTCTCCATTACCGAAGGATG+CGG 0.569694 8_2:-53859201 None:intergenic
GAAACTAAACTAGAACCCTC+TGG 0.570954 8_2:-53858972 None:intergenic
AGCGACCTAATTAGGTCCAT+GGG 0.573274 8_2:+53858648 Msa1255350:CDS
GCACTTATCACATCAATTGG+CGG 0.574617 8_2:+53859149 Msa1255350:CDS
GCTAGCTTCACCTGAAGCTG+TGG 0.576380 8_2:-53856922 None:intergenic
ATCTTGTTGACCAGATGAAG+TGG 0.580608 8_2:-53858919 None:intergenic
ATCACCGCCGGCATCGACAC+CGG 0.580859 8_2:-53857761 None:intergenic
TTGAATATTGTTATTACCAG+AGG 0.581923 8_2:-53856952 None:intergenic
GGATCATGATGAACACAAGT+AGG 0.582243 8_2:-53857308 None:intergenic
TTTGAAGTTGCAATTGACAT+GGG 0.586590 8_2:+53859351 Msa1255350:three_prime_UTR
AATCTTCAACTACATAGAAG+AGG 0.593928 8_2:+53857212 Msa1255350:CDS
TCATATCAAATATTTCCCAA+TGG 0.594960 8_2:+53856881 Msa1255350:exon
CATAGTTGCATATTGCACAT+TGG 0.594977 8_2:-53859400 None:intergenic
AGTCCAGAATCGCCGTCACA+AGG 0.597637 8_2:-53859017 None:intergenic
CTCAGCTCCTCCATCGTCCG+AGG 0.601921 8_2:+53858698 Msa1255350:CDS
GGAGGAGCTGAGAAATCTGA+AGG 0.603033 8_2:-53858687 None:intergenic
CTAATTAGGTCGCTGAACGC+CGG 0.604617 8_2:-53858639 None:intergenic
AAGTGCAGACAAGCCTCCAC+CGG 0.604756 8_2:-53859132 None:intergenic
GCTTTGGAGACTCAGAAGCT+AGG 0.609936 8_2:+53857836 Msa1255350:CDS
ATGATCCATGATTGGTCGAG+AGG 0.611193 8_2:-53858734 None:intergenic
AGGAAAATGTGACCTTAACA+AGG 0.612233 8_2:+53859497 Msa1255350:three_prime_UTR
ACGAGGATCTGTACATCCCA+TGG 0.612346 8_2:-53858664 None:intergenic
CAACAGGTAATGTGCAAATG+GGG 0.617469 8_2:+53859431 Msa1255350:three_prime_UTR
CTTATCACATCAATTGGCGG+TGG 0.618131 8_2:+53859152 Msa1255350:CDS
ACGCATTAAGTGCCAAACAA+TGG 0.618876 8_2:-53859776 None:intergenic
AAATCCACCACAAATCATAG+AGG 0.623951 8_2:+53858452 Msa1255350:CDS
GAAGAGGACATAATCTACCA+TGG 0.626841 8_2:+53857228 Msa1255350:CDS
ATGATTTGGTCCGTAGCTGT+AGG 0.628316 8_2:+53859675 Msa1255350:three_prime_UTR
GAGTCTCCAACCTCAGGCAC+CGG 0.629519 8_2:+53858620 Msa1255350:CDS
TAATAATCTTTGTACAAACA+AGG 0.629763 8_2:-53856813 None:intergenic
AGTACTAGCTCAACAAGCAA+CGG 0.630248 8_2:+53858531 Msa1255350:CDS
TAATGCAGTGGCAGACATCG+CGG 0.630327 8_2:-53858814 None:intergenic
GCTGAGAAATCTGAAGGACG+AGG 0.632449 8_2:-53858681 None:intergenic
GAGATCGCCTCGGACGATGG+AGG 0.640921 8_2:-53858705 None:intergenic
TCTCGGACAAATGGAACCAG+AGG 0.640999 8_2:+53858956 Msa1255350:CDS
AGAGAACTCTCCATTACCGA+AGG 0.648524 8_2:-53859207 None:intergenic
GAAGCCGGTGTCGATGCCGG+CGG 0.649310 8_2:+53857757 Msa1255350:CDS
CGGACCAAATCATCCATCTG+AGG 0.650001 8_2:-53859665 None:intergenic
TCCAAGCCCGCGAAGCAGAG+AGG 0.660190 8_2:-53858877 None:intergenic
GCAGTAGTAGTAATCACCGC+CGG 0.670061 8_2:-53857773 None:intergenic
GAGGCAAAGAGAGATCGCCT+CGG 0.672821 8_2:-53858715 None:intergenic
CTCGGACAAATGGAACCAGA+GGG 0.680399 8_2:+53858957 Msa1255350:CDS
CTTCTGAGTCTCCAAAGCAG+AGG 0.681133 8_2:-53857831 None:intergenic
GCAGTGGCAGACATCGCGGG+CGG 0.685946 8_2:-53858810 None:intergenic
CAACAAGATAGCCTGCATTG+GGG 0.703664 8_2:+53858933 Msa1255350:CDS
TGCCAAACAATGGACAAACG+TGG 0.705749 8_2:-53859766 None:intergenic
GGACTAGGTCTTCCTTGTGA+CGG 0.715099 8_2:+53859005 Msa1255350:CDS
GACACACCACTATTTGACTG+AGG 0.723609 8_2:-53857809 None:intergenic
GTAGTAGTAACAACAACTTG+TGG 0.757320 8_2:-53858477 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTAAATTTTTATTTT+TGG + chr8_2:53857517-53857536 Msa1255350:intron 0.0%
!!! TTTTATATGTTTATGAAAAA+TGG + chr8_2:53857555-53857574 Msa1255350:intron 10.0%
!! TTTATATGTTTATGAAAAAT+GGG + chr8_2:53857556-53857575 Msa1255350:intron 10.0%
!!! AATATTTATTAATTTGTACC+TGG - chr8_2:53857026-53857045 None:intergenic 15.0%
!! AAATTTAATAAAAAAGAACC+TGG - chr8_2:53857510-53857529 None:intergenic 15.0%
!!! CAAATTCAAAATTTTGTTTT+TGG + chr8_2:53857968-53857987 Msa1255350:intron 15.0%
!! TGAAAATAAATCACTTAAAA+AGG + chr8_2:53858119-53858138 Msa1255350:intron 15.0%
!! TTTAATGTAACATAAAAGAA+AGG + chr8_2:53858268-53858287 Msa1255350:intron 15.0%
!! ATTATAATCATCAACTAATA+GGG - chr8_2:53858347-53858366 None:intergenic 15.0%
!! AATTATAATCATCAACTAAT+AGG - chr8_2:53858348-53858367 None:intergenic 15.0%
!! ACAAATCAAAATATAGAATA+AGG - chr8_2:53858417-53858436 None:intergenic 15.0%
!!! GTTTTTTTTTTTGTTTGCTA+GGG - chr8_2:53856742-53856761 None:intergenic 20.0%
!!! TGTTTTTTTTTTTGTTTGCT+AGG - chr8_2:53856743-53856762 None:intergenic 20.0%
!!! AAATCAGTAAGAAACTTTTT+TGG - chr8_2:53856772-53856791 None:intergenic 20.0%
!! TAATAATCTTTGTACAAACA+AGG - chr8_2:53856816-53856835 None:intergenic 20.0%
!! AAAGATCAAGTAAAGAAATT+AGG + chr8_2:53857261-53857280 Msa1255350:CDS 20.0%
!! AAGTAAAGAAATTAGGAAAA+GGG + chr8_2:53857268-53857287 Msa1255350:CDS 20.0%
!!! TTTTATGTGTTTGATTTTGA+AGG + chr8_2:53857609-53857628 Msa1255350:intron 20.0%
! ATAGCTATAGTTTATAGCTT+AGG + chr8_2:53856846-53856865 Msa1255350:five_prime_UTR 25.0%
! TCATATCAAATATTTCCCAA+TGG + chr8_2:53856881-53856900 Msa1255350:exon 25.0%
!! TTGAATATTGTTATTACCAG+AGG - chr8_2:53856955-53856974 None:intergenic 25.0%
!!! AGATTTCTTTTTTTCTTGGT+GGG - chr8_2:53856993-53857012 None:intergenic 25.0%
! CAAGTAAAGAAATTAGGAAA+AGG + chr8_2:53857267-53857286 Msa1255350:CDS 25.0%
! AAAAAAGAACCTGGAAAAAA+CGG - chr8_2:53857501-53857520 None:intergenic 25.0%
! TAATTCAGAATCTGATTCAA+AGG + chr8_2:53857718-53857737 Msa1255350:CDS 25.0%
!!! AAAGTGCAATTTTTGTTTTC+TGG + chr8_2:53857891-53857910 Msa1255350:intron 25.0%
!!! AAGTGCAATTTTTGTTTTCT+GGG + chr8_2:53857892-53857911 Msa1255350:intron 25.0%
!!! GTGTTTAGTATTTTTTTGTC+TGG + chr8_2:53858017-53858036 Msa1255350:intron 25.0%
! GTTTGATCTAAATTATGCAT+TGG + chr8_2:53858156-53858175 Msa1255350:intron 25.0%
!!! AGAAGATTTGTTTTTAAGAG+TGG + chr8_2:53858207-53858226 Msa1255350:intron 25.0%
!! AATCATGGATCATCAATTTT+CGG + chr8_2:53858744-53858763 Msa1255350:CDS 25.0%
!! GAAGTTCATGATAGATATTT+TGG + chr8_2:53859246-53859265 Msa1255350:exon 25.0%
!! AACTACTAGTGTTATTATCA+GGG + chr8_2:53859279-53859298 Msa1255350:three_prime_UTR 25.0%
!!! AAACATTTGTTGTCTATGAA+TGG + chr8_2:53859324-53859343 Msa1255350:three_prime_UTR 25.0%
!! CATTTTCCTTTCCTTAAATA+TGG - chr8_2:53859486-53859505 None:intergenic 25.0%
! ATATGGTTGTCTTAAGATTT+AGG + chr8_2:53859573-53859592 Msa1255350:three_prime_UTR 25.0%
! TATGGTTGTCTTAAGATTTA+GGG + chr8_2:53859574-53859593 Msa1255350:three_prime_UTR 25.0%
!!! CTAGTAGTAGTAGTTTTATT+TGG + chr8_2:53859712-53859731 Msa1255350:three_prime_UTR 25.0%
!!! TAGTAGTTTTATTTGGTTGT+TGG + chr8_2:53859719-53859738 Msa1255350:three_prime_UTR 25.0%
!!! CTAGGGTTTGTGTATTTTTT+TGG - chr8_2:53856725-53856744 None:intergenic 30.0%
CATTGTCTAAATCAACCATT+GGG - chr8_2:53856899-53856918 None:intergenic 30.0%
ACATTGTCTAAATCAACCAT+TGG - chr8_2:53856900-53856919 None:intergenic 30.0%
!!! GAGATTTCTTTTTTTCTTGG+TGG - chr8_2:53856994-53857013 None:intergenic 30.0%
!!! TGGGAGATTTCTTTTTTTCT+TGG - chr8_2:53856997-53857016 None:intergenic 30.0%
AAGAAAAAAAGAAATCTCCC+AGG + chr8_2:53856996-53857015 Msa1255350:CDS 30.0%
GTGTGTGAAATATGTAACAA+AGG + chr8_2:53857173-53857192 Msa1255350:CDS 30.0%
AATCTTCAACTACATAGAAG+AGG + chr8_2:53857212-53857231 Msa1255350:CDS 30.0%
! AAGCACTTTTGTAGAAAACA+TGG + chr8_2:53857365-53857384 Msa1255350:CDS 30.0%
AATATGCTGTTCAATCTGAT+TGG + chr8_2:53857423-53857442 Msa1255350:CDS 30.0%
! AGAGAGTATAAATGTGATTG+TGG + chr8_2:53857473-53857492 Msa1255350:CDS 30.0%
!! TATGTGTTTGATTTTGAAGG+AGG + chr8_2:53857612-53857631 Msa1255350:intron 30.0%
!! ATGTGTTTGATTTTGAAGGA+GGG + chr8_2:53857613-53857632 Msa1255350:intron 30.0%
!!! GTTTTTTGTCATGTTTGATG+TGG + chr8_2:53857928-53857947 Msa1255350:intron 30.0%
!! GGTGTTATTGTTTGTTTATG+TGG + chr8_2:53857989-53858008 Msa1255350:intron 30.0%
ATTGTTTGTTTATGTGGTCT+TGG + chr8_2:53857995-53858014 Msa1255350:intron 30.0%
!! AAATTATGCATTGGTTGTCA+AGG + chr8_2:53858165-53858184 Msa1255350:intron 30.0%
AACACATAGACAAGTAAAGT+TGG - chr8_2:53858296-53858315 None:intergenic 30.0%
! GAAAATTGATGATCCATGAT+TGG - chr8_2:53858745-53858764 None:intergenic 30.0%
GACTAAAGGAATTGATGTTA+GGG + chr8_2:53859036-53859055 Msa1255350:CDS 30.0%
! CAACTACTAGTGTTATTATC+AGG + chr8_2:53859278-53859297 Msa1255350:three_prime_UTR 30.0%
!!! TATTATCAGGGCAACTTTTT+TGG + chr8_2:53859291-53859310 Msa1255350:three_prime_UTR 30.0%
!!! TTTTGAAGTTGCAATTGACA+TGG + chr8_2:53859350-53859369 Msa1255350:three_prime_UTR 30.0%
! TTTGAAGTTGCAATTGACAT+GGG + chr8_2:53859351-53859370 Msa1255350:three_prime_UTR 30.0%
CTTAAATATGGTCTTTCTTC+TGG - chr8_2:53859474-53859493 None:intergenic 30.0%
CAGAAGAAAGACCATATTTA+AGG + chr8_2:53859472-53859491 Msa1255350:three_prime_UTR 30.0%
GAAAGACCATATTTAAGGAA+AGG + chr8_2:53859477-53859496 Msa1255350:three_prime_UTR 30.0%
! AAATTAAGGGTATGTGCATA+TGG + chr8_2:53859556-53859575 Msa1255350:three_prime_UTR 30.0%
!!! ATGGTCTTGTTTTTGGTTTA+TGG + chr8_2:53859618-53859637 Msa1255350:three_prime_UTR 30.0%
CTAATTTATACCTACAGCTA+CGG - chr8_2:53859688-53859707 None:intergenic 30.0%
TGGAATTATTGTCACATTTG+TGG + chr8_2:53859739-53859758 Msa1255350:three_prime_UTR 30.0%
!!! CTTTTTTTCTTGGTGGGTTT+TGG - chr8_2:53856987-53857006 None:intergenic 35.0%
TTTGTAGCTAACAACGTTGT+TGG - chr8_2:53857146-53857165 None:intergenic 35.0%
!! AAATCACCTAAAGCTCTAGA+AGG - chr8_2:53857332-53857351 None:intergenic 35.0%
!! AGAGCTTTAGGTGATTTAAC+TGG + chr8_2:53857335-53857354 Msa1255350:CDS 35.0%
!! GAGCTTTAGGTGATTTAACT+GGG + chr8_2:53857336-53857355 Msa1255350:CDS 35.0%
GAAAACATGGTGAGAAAAAG+TGG + chr8_2:53857378-53857397 Msa1255350:CDS 35.0%
!! AAAGCTCACTCTAAAGTTTG+TGG + chr8_2:53857446-53857465 Msa1255350:CDS 35.0%
!! TCTGATTCAAAGGTTTTGAC+CGG + chr8_2:53857728-53857747 Msa1255350:CDS 35.0%
!!! GACTTGTTTTTGTGAGAAAC+AGG + chr8_2:53858056-53858075 Msa1255350:intron 35.0%
ACTTGTCTATGTGTTATGAG+TGG + chr8_2:53858301-53858320 Msa1255350:intron 35.0%
!! TTGATTTGTTGCAGATTTGC+CGG + chr8_2:53858428-53858447 Msa1255350:intron 35.0%
!!! ATGATTTGTGGTGGATTTTC+CGG - chr8_2:53858450-53858469 None:intergenic 35.0%
AAATCCACCACAAATCATAG+AGG + chr8_2:53858452-53858471 Msa1255350:CDS 35.0%
GTAGTAGTAACAACAACTTG+TGG - chr8_2:53858480-53858499 None:intergenic 35.0%
!!! TGCTTTTTGAAGTAATGCAG+TGG - chr8_2:53858829-53858848 None:intergenic 35.0%
ACTTCAAAAAGCAGCTCAAA+TGG + chr8_2:53858836-53858855 Msa1255350:CDS 35.0%
CTTCAAAAAGCAGCTCAAAT+GGG + chr8_2:53858837-53858856 Msa1255350:CDS 35.0%
GGACTAAAGGAATTGATGTT+AGG + chr8_2:53859035-53859054 Msa1255350:CDS 35.0%
AAATCAAGAGTTGTCTGCTT+AGG - chr8_2:53859080-53859099 None:intergenic 35.0%
AACTCTTGATTTCCTAGGAT+TGG + chr8_2:53859088-53859107 Msa1255350:CDS 35.0%
ACTCTTGATTTCCTAGGATT+GGG + chr8_2:53859089-53859108 Msa1255350:CDS 35.0%
TCTGCACTTATCACATCAAT+TGG + chr8_2:53859146-53859165 Msa1255350:CDS 35.0%
CATAGTTGCATATTGCACAT+TGG - chr8_2:53859403-53859422 None:intergenic 35.0%
CAATGTGCAATATGCAACTA+TGG + chr8_2:53859401-53859420 Msa1255350:three_prime_UTR 35.0%
GTCTTTCTTCTGGCATTATA+AGG - chr8_2:53859464-53859483 None:intergenic 35.0%
AGGAAAATGTGACCTTAACA+AGG + chr8_2:53859497-53859516 Msa1255350:three_prime_UTR 35.0%
AAGCACATTGTACCTTGTTA+AGG - chr8_2:53859512-53859531 None:intergenic 35.0%
! AAGGTACAATGTGCTTGTTT+TGG + chr8_2:53859516-53859535 Msa1255350:three_prime_UTR 35.0%
!!! GAAGATCATGGTCTTGTTTT+TGG + chr8_2:53859611-53859630 Msa1255350:three_prime_UTR 35.0%
CAAATCATGTATTCCTCAGA+TGG + chr8_2:53859652-53859671 Msa1255350:three_prime_UTR 35.0%
TATTCCTCAGATGGATGATT+TGG + chr8_2:53859661-53859680 Msa1255350:three_prime_UTR 35.0%
CGTAGCTGTAGGTATAAATT+AGG + chr8_2:53859686-53859705 Msa1255350:three_prime_UTR 35.0%
!!! TTCTTGGTGGGTTTTGGAAT+TGG - chr8_2:53856981-53857000 None:intergenic 40.0%
AATTTGTACCTGGCATTCCT+GGG - chr8_2:53857016-53857035 None:intergenic 40.0%
! TAATTTGTACCTGGCATTCC+TGG - chr8_2:53857017-53857036 None:intergenic 40.0%
!! TTTTGAAGATCCAGAAGCAG+AGG + chr8_2:53857106-53857125 Msa1255350:intron 40.0%
GAAGAGGACATAATCTACCA+TGG + chr8_2:53857228-53857247 Msa1255350:CDS 40.0%
GATCTTTGTCTCAGTTTCCA+TGG - chr8_2:53857248-53857267 None:intergenic 40.0%
TGATGAACACAAGTAGGTTC+AGG - chr8_2:53857305-53857324 None:intergenic 40.0%
GGATCATGATGAACACAAGT+AGG - chr8_2:53857311-53857330 None:intergenic 40.0%
!! CATGATCCTTCTAGAGCTTT+AGG + chr8_2:53857323-53857342 Msa1255350:CDS 40.0%
!!! ATTGTGGAACCGTTTTTTCC+AGG + chr8_2:53857489-53857508 Msa1255350:CDS 40.0%
! TAGAGCTTTTTGTGATGCGT+TGG + chr8_2:53857652-53857671 Msa1255350:CDS 40.0%
ACTACTCCTCAGTCAAATAG+TGG + chr8_2:53857803-53857822 Msa1255350:CDS 40.0%
! GGATCAAGTGATCTGAGATT+CGG + chr8_2:53858077-53858096 Msa1255350:intron 40.0%
! GATCAAGTGATCTGAGATTC+GGG + chr8_2:53858078-53858097 Msa1255350:intron 40.0%
! ATCAAGTGATCTGAGATTCG+GGG + chr8_2:53858079-53858098 Msa1255350:intron 40.0%
AAGGAAAAGCAAAGCAAAGC+AGG - chr8_2:53858398-53858417 None:intergenic 40.0%
AGTACTAGCTCAACAAGCAA+CGG + chr8_2:53858531-53858550 Msa1255350:CDS 40.0%
TACATCCCATGGACCTAATT+AGG - chr8_2:53858656-53858675 None:intergenic 40.0%
! GGATCATCAATTTTCGGAAC+AGG + chr8_2:53858750-53858769 Msa1255350:CDS 40.0%
! TCATCAATTTTCGGAACAGG+AGG + chr8_2:53858753-53858772 Msa1255350:CDS 40.0%
! CATCAATTTTCGGAACAGGA+GGG + chr8_2:53858754-53858773 Msa1255350:CDS 40.0%
ATCTTGTTGACCAGATGAAG+TGG - chr8_2:53858922-53858941 None:intergenic 40.0%
TCAACAAGATAGCCTGCATT+GGG + chr8_2:53858932-53858951 Msa1255350:CDS 40.0%
GAAACTAAACTAGAACCCTC+TGG - chr8_2:53858975-53858994 None:intergenic 40.0%
!! GGTTCTAGTTTAGTTTCAGC+AGG + chr8_2:53858978-53858997 Msa1255350:CDS 40.0%
! AGTTTAGTTTCAGCAGGACT+CGG + chr8_2:53858984-53859003 Msa1255350:CDS 40.0%
TGCTTAGGACCAAACATTGA+CGG - chr8_2:53859065-53859084 None:intergenic 40.0%
CAGACAACTCTTGATTTCCT+AGG + chr8_2:53859083-53859102 Msa1255350:CDS 40.0%
CTCTTGATTTCCTAGGATTG+GGG + chr8_2:53859090-53859109 Msa1255350:CDS 40.0%
GCACTTATCACATCAATTGG+CGG + chr8_2:53859149-53859168 Msa1255350:CDS 40.0%
GAGTTCTCTGGCAAAGATAT+TGG + chr8_2:53859221-53859240 Msa1255350:CDS 40.0%
CAACTATGGATCAATGCAAC+AGG + chr8_2:53859415-53859434 Msa1255350:three_prime_UTR 40.0%
TGCAACAGGTAATGTGCAAA+TGG + chr8_2:53859429-53859448 Msa1255350:three_prime_UTR 40.0%
GCAACAGGTAATGTGCAAAT+GGG + chr8_2:53859430-53859449 Msa1255350:three_prime_UTR 40.0%
CAACAGGTAATGTGCAAATG+GGG + chr8_2:53859431-53859450 Msa1255350:three_prime_UTR 40.0%
ACACAGGCTGTGAGAAATTA+AGG + chr8_2:53859542-53859561 Msa1255350:three_prime_UTR 40.0%
CACAGGCTGTGAGAAATTAA+GGG + chr8_2:53859543-53859562 Msa1255350:three_prime_UTR 40.0%
GTCTTAAGATTTAGGGACAG+TGG + chr8_2:53859581-53859600 Msa1255350:three_prime_UTR 40.0%
! AGTGGAGCATTTGAAGATCA+TGG + chr8_2:53859599-53859618 Msa1255350:three_prime_UTR 40.0%
TTCCACGTTTGTCCATTGTT+TGG + chr8_2:53859764-53859783 Msa1255350:three_prime_UTR 40.0%
ACGCATTAAGTGCCAAACAA+TGG - chr8_2:53859779-53859798 None:intergenic 40.0%
GACAATGTTTCCACAGCTTC+AGG + chr8_2:53856912-53856931 Msa1255350:CDS 45.0%
!! TTTTTGTGATGCGTTGGCAG+AGG + chr8_2:53857658-53857677 Msa1255350:CDS 45.0%
GACACACCACTATTTGACTG+AGG - chr8_2:53857812-53857831 None:intergenic 45.0%
TGTGGCTCCTCTATGATTTG+TGG - chr8_2:53858462-53858481 None:intergenic 45.0%
AGCGACCTAATTAGGTCCAT+GGG + chr8_2:53858648-53858667 Msa1255350:CDS 45.0%
! ATGATCCATGATTGGTCGAG+AGG - chr8_2:53858737-53858756 None:intergenic 45.0%
TCTTCTGCTTCCACTTCATC+TGG + chr8_2:53858909-53858928 Msa1255350:CDS 45.0%
GTCAACAAGATAGCCTGCAT+TGG + chr8_2:53858931-53858950 Msa1255350:CDS 45.0%
CAACAAGATAGCCTGCATTG+GGG + chr8_2:53858933-53858952 Msa1255350:CDS 45.0%
TTAGGGACTCCGTCAATGTT+TGG + chr8_2:53859053-53859072 Msa1255350:CDS 45.0%
TGATTTCCTAGGATTGGGGA+TGG + chr8_2:53859094-53859113 Msa1255350:CDS 45.0%
CTTATCACATCAATTGGCGG+TGG + chr8_2:53859152-53859171 Msa1255350:CDS 45.0%
AGAGAACTCTCCATTACCGA+AGG - chr8_2:53859210-53859229 None:intergenic 45.0%
!! TTCGGTAATGGAGAGTTCTC+TGG + chr8_2:53859209-53859228 Msa1255350:CDS 45.0%
!!! GTGCTTGTTTTGGACAACAC+AGG + chr8_2:53859526-53859545 Msa1255350:three_prime_UTR 45.0%
!! ATGATTTGGTCCGTAGCTGT+AGG + chr8_2:53859675-53859694 Msa1255350:three_prime_UTR 45.0%
TGCCAAACAATGGACAAACG+TGG - chr8_2:53859769-53859788 None:intergenic 45.0%
GAAGCTAGCATCTCTTCCTC+TGG + chr8_2:53856936-53856955 Msa1255350:CDS 50.0%
AGAAATCTCCCAGGAATGCC+AGG + chr8_2:53857005-53857024 Msa1255350:CDS 50.0%
AGAGCAATCACCTCTGCTTC+TGG - chr8_2:53857119-53857138 None:intergenic 50.0%
TTTGACCGGTGATTCGAAGC+CGG + chr8_2:53857742-53857761 Msa1255350:CDS 50.0%
GCAGTAGTAGTAATCACCGC+CGG - chr8_2:53857776-53857795 None:intergenic 50.0%
TAGTGGTGTGTCCTCTGCTT+TGG + chr8_2:53857820-53857839 Msa1255350:CDS 50.0%
CTTCTGAGTCTCCAAAGCAG+AGG - chr8_2:53857834-53857853 None:intergenic 50.0%
!! GCTTTGGAGACTCAGAAGCT+AGG + chr8_2:53857836-53857855 Msa1255350:CDS 50.0%
! GGCTCCTCTATGATTTGTGG+TGG - chr8_2:53858459-53858478 None:intergenic 50.0%
CTAATTAGGTCGCTGAACGC+CGG - chr8_2:53858642-53858661 None:intergenic 50.0%
CAGCGACCTAATTAGGTCCA+TGG + chr8_2:53858647-53858666 Msa1255350:CDS 50.0%
ACGAGGATCTGTACATCCCA+TGG - chr8_2:53858667-53858686 None:intergenic 50.0%
GCTGAGAAATCTGAAGGACG+AGG - chr8_2:53858684-53858703 None:intergenic 50.0%
GGAGGAGCTGAGAAATCTGA+AGG - chr8_2:53858690-53858709 None:intergenic 50.0%
CTTTGCCTCTCGACCAATCA+TGG + chr8_2:53858729-53858748 Msa1255350:CDS 50.0%
! AATTTTCGGAACAGGAGGGC+AGG + chr8_2:53858758-53858777 Msa1255350:CDS 50.0%
! GCTGATGTGTTGGCACGTAT+TGG - chr8_2:53858791-53858810 None:intergenic 50.0%
TAATGCAGTGGCAGACATCG+CGG - chr8_2:53858817-53858836 None:intergenic 50.0%
TCTCGGACAAATGGAACCAG+AGG + chr8_2:53858956-53858975 Msa1255350:CDS 50.0%
CTCGGACAAATGGAACCAGA+GGG + chr8_2:53858957-53858976 Msa1255350:CDS 50.0%
GGACTAGGTCTTCCTTGTGA+CGG + chr8_2:53859005-53859024 Msa1255350:CDS 50.0%
TGACGGCGATTCTGGACTAA+AGG + chr8_2:53859022-53859041 Msa1255350:CDS 50.0%
CTCTCCATTACCGAAGGATG+CGG - chr8_2:53859204-53859223 None:intergenic 50.0%
CGGACCAAATCATCCATCTG+AGG - chr8_2:53859668-53859687 None:intergenic 50.0%
! GCTAGCTTCACCTGAAGCTG+TGG - chr8_2:53856925-53856944 None:intergenic 55.0%
TTCTGCGAGTCTCCAACCTC+AGG + chr8_2:53858614-53858633 Msa1255350:CDS 55.0%
CGGCGTTCAGCGACCTAATT+AGG + chr8_2:53858640-53858659 Msa1255350:CDS 55.0%
GAGGCAAAGAGAGATCGCCT+CGG - chr8_2:53858718-53858737 None:intergenic 55.0%
AATGCAGTGGCAGACATCGC+GGG - chr8_2:53858816-53858835 None:intergenic 55.0%
ACAAACGCCTCTCTGCTTCG+CGG + chr8_2:53858870-53858889 Msa1255350:CDS 55.0%
GATAGCCTGCATTGGGGTCT+CGG + chr8_2:53858939-53858958 Msa1255350:CDS 55.0%
TTTGTCCGAGACCCCAATGC+AGG - chr8_2:53858947-53858966 None:intergenic 55.0%
GCATTGGGGTCTCGGACAAA+TGG + chr8_2:53858947-53858966 Msa1255350:CDS 55.0%
GTTTCAGCAGGACTCGGACT+AGG + chr8_2:53858990-53859009 Msa1255350:CDS 55.0%
CTTCCTTGTGACGGCGATTC+TGG + chr8_2:53859014-53859033 Msa1255350:CDS 55.0%
AGTCCAGAATCGCCGTCACA+AGG - chr8_2:53859020-53859039 None:intergenic 55.0%
AAGTGCAGACAAGCCTCCAC+CGG - chr8_2:53859135-53859154 None:intergenic 55.0%
ACATCAATTGGCGGTGGCAG+TGG + chr8_2:53859158-53859177 Msa1255350:CDS 55.0%
!! CGACACCGGCTTCGAATCAC+CGG - chr8_2:53857750-53857769 None:intergenic 60.0%
!! TTCGAAGCCGGTGTCGATGC+CGG + chr8_2:53857754-53857773 Msa1255350:CDS 60.0%
GAGTCTCCAACCTCAGGCAC+CGG + chr8_2:53858620-53858639 Msa1255350:CDS 60.0%
AGAGAGATCGCCTCGGACGA+TGG - chr8_2:53858711-53858730 None:intergenic 60.0%
CAAACGCCTCTCTGCTTCGC+GGG + chr8_2:53858871-53858890 Msa1255350:CDS 60.0%
CAGCAGCCATCCCCAATCCT+AGG - chr8_2:53859103-53859122 None:intergenic 60.0%
CTAGGATTGGGGATGGCTGC+TGG + chr8_2:53859101-53859120 Msa1255350:CDS 60.0%
AATTGGCGGTGGCAGTGGTC+TGG + chr8_2:53859163-53859182 Msa1255350:CDS 60.0%
GCAGCCGCATCCTTCGGTAA+TGG + chr8_2:53859197-53859216 Msa1255350:CDS 60.0%
!! ATCACCGCCGGCATCGACAC+CGG - chr8_2:53857764-53857783 None:intergenic 65.0%
GGCTGTGCTACCACCAGCAC+TGG + chr8_2:53858552-53858571 Msa1255350:CDS 65.0%
! CGCAAACACGCCAGTGCTGG+TGG - chr8_2:53858565-53858584 None:intergenic 65.0%
GCTCGCAAACACGCCAGTGC+TGG - chr8_2:53858568-53858587 None:intergenic 65.0%
TGAACGCCGGTGCCTGAGGT+TGG - chr8_2:53858629-53858648 None:intergenic 65.0%
CTCAGCTCCTCCATCGTCCG+AGG + chr8_2:53858698-53858717 Msa1255350:CDS 65.0%
GAGATCGCCTCGGACGATGG+AGG - chr8_2:53858708-53858727 None:intergenic 65.0%
ATCGCGGGCGGCTGATGTGT+TGG - chr8_2:53858801-53858820 None:intergenic 65.0%
TCCAAGCCCGCGAAGCAGAG+AGG - chr8_2:53858880-53858899 None:intergenic 65.0%
GGATTGGGGATGGCTGCTGG+TGG + chr8_2:53859104-53859123 Msa1255350:CDS 65.0%
ATGGCTGCTGGTGGCAGTGC+CGG + chr8_2:53859113-53859132 Msa1255350:CDS 65.0%
ACCGCAGCAGCCGCATCCTT+CGG + chr8_2:53859191-53859210 Msa1255350:CDS 65.0%
ACCGAAGGATGCGGCTGCTG+CGG - chr8_2:53859195-53859214 None:intergenic 65.0%
!! GAAGCCGGTGTCGATGCCGG+CGG + chr8_2:53857757-53857776 Msa1255350:CDS 70.0%
TCGCTGAACGCCGGTGCCTG+AGG - chr8_2:53858633-53858652 None:intergenic 70.0%
GCAGTGGCAGACATCGCGGG+CGG - chr8_2:53858813-53858832 None:intergenic 70.0%
GCCTCTCTGCTTCGCGGGCT+TGG + chr8_2:53858876-53858895 Msa1255350:CDS 70.0%
! GCTGCTGGTGGCAGTGCCGG+TGG + chr8_2:53859116-53859135 Msa1255350:CDS 75.0%
!! GCTGGTGGCAGTGCCGGTGG+AGG + chr8_2:53859119-53859138 Msa1255350:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 53856696 53859830 53856696 ID=Msa1255350;Name=Msa1255350
chr8_2 mRNA 53856696 53859830 53856696 ID=Msa1255350-mRNA-1;Parent=Msa1255350;Name=Msa1255350-mRNA-1;_AED=0.00;_eAED=0.00;_QI=204|1|1|1|1|1|4|574|521
chr8_2 exon 53856696 53857026 53856696 ID=Msa1255350-mRNA-1:exon:16690;Parent=Msa1255350-mRNA-1
chr8_2 exon 53857114 53857510 53857114 ID=Msa1255350-mRNA-1:exon:16691;Parent=Msa1255350-mRNA-1
chr8_2 exon 53857631 53857857 53857631 ID=Msa1255350-mRNA-1:exon:16692;Parent=Msa1255350-mRNA-1
chr8_2 exon 53858442 53859830 53858442 ID=Msa1255350-mRNA-1:exon:16693;Parent=Msa1255350-mRNA-1
chr8_2 five_prime_UTR 53856696 53856899 53856696 ID=Msa1255350-mRNA-1:five_prime_utr;Parent=Msa1255350-mRNA-1
chr8_2 CDS 53856900 53857026 53856900 ID=Msa1255350-mRNA-1:cds;Parent=Msa1255350-mRNA-1
chr8_2 CDS 53857114 53857510 53857114 ID=Msa1255350-mRNA-1:cds;Parent=Msa1255350-mRNA-1
chr8_2 CDS 53857631 53857857 53857631 ID=Msa1255350-mRNA-1:cds;Parent=Msa1255350-mRNA-1
chr8_2 CDS 53858442 53859256 53858442 ID=Msa1255350-mRNA-1:cds;Parent=Msa1255350-mRNA-1
chr8_2 three_prime_UTR 53859257 53859830 53859257 ID=Msa1255350-mRNA-1:three_prime_utr;Parent=Msa1255350-mRNA-1
Gene Sequence

>Msa1255350

ATGGTTGATTTAGACAATGTTTCCACAGCTTCAGGTGAAGCTAGCATCTCTTCCTCTGGTAATAACAATATTCAATCACCAATTCCAAAACCCACCAAGAAAAAAAGAAATCTCCCAGGAATGCCAGATCCAGAAGCAGAGGTGATTGCTCTGTCACCAACAACGTTGTTAGCTACAAACAGATTTGTGTGTGAAATATGTAACAAAGGTTTTCAAAGAGATCAGAATCTTCAACTACATAGAAGAGGACATAATCTACCATGGAAACTGAGACAAAGATCAAGTAAAGAAATTAGGAAAAGGGTTTATGTTTGTCCTGAACCTACTTGTGTTCATCATGATCCTTCTAGAGCTTTAGGTGATTTAACTGGGATAAAAAAGCACTTTTGTAGAAAACATGGTGAGAAAAAGTGGAAATGTGAGAAATGTTCTAAGAAATATGCTGTTCAATCTGATTGGAAAGCTCACTCTAAAGTTTGTGGTTCTAGAGAGTATAAATGTGATTGTGGAACCGTTTTTTCCAGGAGGGATAGTTTTATCACACATAGAGCTTTTTGTGATGCGTTGGCAGAGGAGAATGCAAAATCTCAGAATCAAGCTGTTGTGAAAGCTAATTCAGAATCTGATTCAAAGGTTTTGACCGGTGATTCGAAGCCGGTGTCGATGCCGGCGGTGATTACTACTACTGCTGCTGCTACTACTCCTCAGTCAAATAGTGGTGTGTCCTCTGCTTTGGAGACTCAGAAGCTAGATTTGCCGGAAAATCCACCACAAATCATAGAGGAGCCACAAGTTGTTGTTACTACTACTGCTAGTGCTTTAAATGCGAGTTGTAGCAGCAGTACTAGCTCAACAAGCAACGGCTGTGCTACCACCAGCACTGGCGTGTTTGCGAGCTTGTTTGCTTCATCAACAGCTTCAGCTTCTGCGAGTCTCCAACCTCAGGCACCGGCGTTCAGCGACCTAATTAGGTCCATGGGATGTACAGATCCTCGTCCTTCAGATTTCTCAGCTCCTCCATCGTCCGAGGCGATCTCTCTTTGCCTCTCGACCAATCATGGATCATCAATTTTCGGAACAGGAGGGCAGGAGTGTCGCCAATACGTGCCAACACATCAGCCGCCCGCGATGTCTGCCACTGCATTACTTCAAAAAGCAGCTCAAATGGGCGCTGCTGCAACAAACGCCTCTCTGCTTCGCGGGCTTGGAATTGTGTCGTCTTCTGCTTCCACTTCATCTGGTCAACAAGATAGCCTGCATTGGGGTCTCGGACAAATGGAACCAGAGGGTTCTAGTTTAGTTTCAGCAGGACTCGGACTAGGTCTTCCTTGTGACGGCGATTCTGGACTAAAGGAATTGATGTTAGGGACTCCGTCAATGTTTGGTCCTAAGCAGACAACTCTTGATTTCCTAGGATTGGGGATGGCTGCTGGTGGCAGTGCCGGTGGAGGCTTGTCTGCACTTATCACATCAATTGGCGGTGGCAGTGGTCTGGATGTTACCGCAGCAGCCGCATCCTTCGGTAATGGAGAGTTCTCTGGCAAAGATATTGGAAGAAGTTCATGA
Protein sequence

>Msa1255350

MVDLDNVSTASGEASISSSGNNNIQSPIPKPTKKKRNLPGMPDPEAEVIALSPTTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLRQRSSKEIRKRVYVCPEPTCVHHDPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKKYAVQSDWKAHSKVCGSREYKCDCGTVFSRRDSFITHRAFCDALAEENAKSQNQAVVKANSESDSKVLTGDSKPVSMPAVITTTAAATTPQSNSGVSSALETQKLDLPENPPQIIEEPQVVVTTTASALNASCSSSTSSTSNGCATTSTGVFASLFASSTASASASLQPQAPAFSDLIRSMGCTDPRPSDFSAPPSSEAISLCLSTNHGSSIFGTGGQECRQYVPTHQPPAMSATALLQKAAQMGAAATNASLLRGLGIVSSSASTSSGQQDSLHWGLGQMEPEGSSLVSAGLGLGLPCDGDSGLKELMLGTPSMFGPKQTTLDFLGLGMAAGGSAGGGLSALITSIGGGSGLDVTAAAASFGNGEFSGKDIGRSS*