AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1255810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1255810 MtrunA17_Chr8g0374601 73.664 262 53 2 1 246 19 280 1.57e-137 388
Msa1255810 MtrunA17_Chr8g0374591 88.889 126 14 0 1 126 1 126 1.53e-80 238
Msa1255810 MtrunA17_Chr8g0374581 50.000 174 79 3 1 171 1 169 1.80e-52 170
Msa1255810 MtrunA17_Chr5g0402611 55.856 111 49 0 8 118 8 118 1.82e-38 136
Msa1255810 MtrunA17_Chr3g0115431 53.636 110 51 0 8 117 9 118 7.24e-38 132
Msa1255810 MtrunA17_Chr3g0097221 45.562 169 70 4 8 155 7 174 7.70e-37 131
Msa1255810 MtrunA17_Chr4g0058651 50.962 104 51 0 8 111 11 114 1.68e-33 120
Msa1255810 MtrunA17_Chr3g0117121 47.706 109 57 0 8 116 10 118 1.45e-32 117
Msa1255810 MtrunA17_Chr7g0227511 47.170 106 56 0 8 113 11 116 5.18e-32 115
Msa1255810 MtrunA17_Chr2g0311601 48.544 103 53 0 6 108 11 113 3.67e-31 113
Msa1255810 MtrunA17_Chr3g0106101 44.554 101 56 0 8 108 7 107 7.21e-31 113
Msa1255810 MtrunA17_Chr5g0437401 49.020 102 52 0 9 110 12 113 2.73e-30 111
Msa1255810 MtrunA17_Chr5g0437571 48.515 101 52 0 8 108 6 106 2.90e-30 111
Msa1255810 MtrunA17_Chr3g0114061 46.535 101 54 0 8 108 8 108 4.51e-30 110
Msa1255810 MtrunA17_Chr8g0353981 52.885 104 49 0 8 111 7 110 6.53e-30 112
Msa1255810 MtrunA17_Chr4g0043421 43.165 139 72 3 8 146 15 146 1.31e-29 109
Msa1255810 MtrunA17_Chr6g0449651 44.231 104 58 0 8 111 67 170 1.24e-28 107
Msa1255810 MtrunA17_Chr6g0449641 45.631 103 56 0 8 110 9 111 1.58e-28 105
Msa1255810 MtrunA17_Chr4g0030001 39.416 137 80 3 5 140 21 155 4.23e-27 103
Msa1255810 MtrunA17_Chr5g0438091 44.231 104 58 0 8 111 39 142 4.76e-27 103
Msa1255810 MtrunA17_Chr3g0129071 44.340 106 59 0 6 111 45 150 6.81e-27 104
Msa1255810 MtrunA17_Chr1g0200201 46.535 101 54 0 9 109 73 173 4.14e-26 102
Msa1255810 MtrunA17_Chr3g0113891 47.423 97 51 0 9 105 15 111 6.54e-26 100
Msa1255810 MtrunA17_Chr5g0432451 42.609 115 66 0 6 120 41 155 2.44e-25 100
Msa1255810 MtrunA17_Chr4g0055441 46.078 102 54 1 9 109 3 104 3.24e-25 97.8
Msa1255810 MtrunA17_Chr7g0225111 38.095 105 65 0 5 109 2 106 1.27e-24 95.1
Msa1255810 MtrunA17_Chr7g0260971 44.660 103 56 1 8 109 14 116 1.76e-24 97.4
Msa1255810 MtrunA17_Chr8g0351701 46.667 105 55 1 8 111 23 127 2.19e-24 97.4
Msa1255810 MtrunA17_Chr6g0460951 41.667 108 62 1 2 108 34 141 2.21e-24 97.4
Msa1255810 MtrunA17_Chr5g0444351 38.614 101 62 0 9 109 6 106 6.37e-24 94.0
Msa1255810 MtrunA17_Chr6g0460941 46.739 92 48 1 4 94 3 94 1.85e-23 91.3
Msa1255810 MtrunA17_Chr8g0342351 38.835 103 63 0 9 111 13 115 1.13e-22 93.6
Msa1255810 MtrunA17_Chr6g0452691 42.308 104 59 1 7 109 8 111 1.53e-22 91.7
Msa1255810 MtrunA17_Chr1g0184281 43.564 101 56 1 8 107 15 115 6.58e-22 90.5
Msa1255810 MtrunA17_Chr4g0059901 40.196 102 61 0 7 108 9 110 1.28e-21 90.9
Msa1255810 MtrunA17_Chr7g0245871 45.098 102 55 1 8 108 27 128 1.99e-21 90.1
Msa1255810 MtrunA17_Chr6g0452661 40.367 109 61 2 5 109 3 111 2.49e-21 89.7
Msa1255810 MtrunA17_Chr8g0351721 44.660 103 56 1 8 109 18 120 4.33e-21 88.6
Msa1255810 MtrunA17_Chr1g0184271 44.231 104 57 1 8 110 12 115 5.19e-21 88.6
Msa1255810 MtrunA17_Chr5g0439111 41.284 109 60 2 5 109 3 111 4.60e-20 86.3
Msa1255810 MtrunA17_Chr7g0261031 41.346 104 60 1 8 110 8 111 4.67e-20 85.9
Msa1255810 MtrunA17_Chr6g0452671 36.364 99 63 0 7 105 10 108 7.30e-20 84.7
Msa1255810 MtrunA17_Chr4g0040831 33.607 122 74 1 1 115 1 122 1.28e-18 81.6
Msa1255810 MtrunA17_Chr5g0435641 38.835 103 62 1 9 110 8 110 2.00e-18 80.9
Msa1255810 MtrunA17_Chr2g0330201 33.981 103 68 0 7 109 18 120 4.57e-18 81.6
Msa1255810 MtrunA17_Chr4g0046201 37.209 86 54 0 9 94 7 92 2.20e-17 79.3
Msa1255810 MtrunA17_Chr7g0245861 44.828 87 45 2 8 91 12 98 1.38e-15 71.6
Msa1255810 MtrunA17_Chr2g0326041 50.000 50 25 0 5 54 30 79 5.15e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1255810 AT5G66870.1 44.872 156 73 2 8 163 7 149 4.08e-36 130
Msa1255810 AT1G65620.1 58.491 106 44 0 8 113 9 114 4.96e-34 122
Msa1255810 AT1G65620.4 58.491 106 44 0 8 113 9 114 4.96e-34 122
Msa1255810 AT1G65620.3 58.491 106 44 0 8 113 9 114 4.96e-34 122
Msa1255810 AT1G65620.5 58.491 106 44 0 8 113 9 114 4.96e-34 122
Msa1255810 AT1G65620.2 58.491 106 44 0 8 113 9 114 4.96e-34 122
Msa1255810 AT5G63090.4 51.923 104 50 0 8 111 11 114 5.80e-33 119
Msa1255810 AT5G63090.3 51.923 104 50 0 8 111 11 114 5.80e-33 119
Msa1255810 AT5G63090.2 51.923 104 50 0 8 111 11 114 5.80e-33 119
Msa1255810 AT5G63090.1 51.923 104 50 0 8 111 11 114 5.80e-33 119
Msa1255810 AT2G23660.2 52.727 110 52 0 8 117 5 114 9.64e-33 122
Msa1255810 AT2G23660.3 52.727 110 52 0 8 117 5 114 9.64e-33 122
Msa1255810 AT2G23660.1 52.727 110 52 0 8 117 5 114 9.64e-33 122
Msa1255810 AT3G27650.1 48.077 104 54 0 8 111 39 142 6.93e-30 110
Msa1255810 AT2G30130.1 47.525 101 53 0 8 108 8 108 9.48e-30 110
Msa1255810 AT1G07900.1 45.283 106 58 0 3 108 28 133 8.70e-29 108
Msa1255810 AT1G16530.1 48.077 104 53 1 6 108 12 115 1.21e-28 107
Msa1255810 AT2G28500.1 46.154 104 56 0 5 108 52 155 1.73e-28 108
Msa1255810 AT1G31320.1 51.136 88 43 0 8 95 13 100 4.22e-28 105
Msa1255810 AT3G26660.1 42.574 101 58 0 9 109 6 106 3.05e-26 99.8
Msa1255810 AT3G26620.1 42.574 101 58 0 9 109 6 106 3.90e-26 99.4
Msa1255810 AT2G30340.2 44.340 106 59 0 6 111 51 156 4.23e-26 103
Msa1255810 AT2G30340.1 44.340 106 59 0 6 111 50 155 4.65e-26 103
Msa1255810 AT3G11090.1 44.000 100 56 0 9 108 12 111 6.48e-26 100
Msa1255810 AT2G40470.2 43.103 116 66 0 6 121 18 133 6.83e-26 100
Msa1255810 AT3G50510.1 43.243 111 62 1 1 111 6 115 1.28e-25 100
Msa1255810 AT3G50510.2 43.243 111 62 1 1 111 6 115 1.28e-25 100
Msa1255810 AT2G40470.1 43.103 116 66 0 6 121 43 158 2.42e-25 100
Msa1255810 AT5G35900.1 44.231 104 58 0 9 112 6 109 9.44e-25 98.2
Msa1255810 AT3G47870.1 40.952 105 62 0 9 113 37 141 6.59e-24 98.6
Msa1255810 AT4G00210.1 45.631 103 55 1 8 109 11 113 1.51e-22 92.8
Msa1255810 AT3G03760.1 45.098 102 55 1 8 108 51 152 1.55e-22 94.0
Msa1255810 AT1G72980.1 39.000 100 61 0 9 108 14 113 2.57e-22 92.0
Msa1255810 AT3G13850.1 38.835 103 63 0 9 111 37 139 1.23e-21 91.3
Msa1255810 AT2G42430.1 40.777 103 60 1 8 109 15 117 3.94e-21 89.7
Msa1255810 AT3G58190.1 43.269 104 58 1 8 110 11 114 5.84e-21 88.6
Msa1255810 AT2G45420.1 44.000 100 55 1 11 109 40 139 3.29e-20 87.4
Msa1255810 AT2G42440.1 39.423 104 62 1 8 110 7 110 7.68e-20 85.9
Msa1255810 AT4G00220.1 45.000 100 54 1 11 109 20 119 1.03e-19 85.5
Msa1255810 AT2G45410.1 44.000 100 55 1 11 109 19 118 5.63e-19 82.4
Msa1255810 AT4G22700.1 37.302 126 75 4 9 131 6 130 3.69e-17 77.8
Msa1255810 AT2G31310.1 37.500 104 64 1 8 110 7 110 6.29e-17 77.0
Msa1255810 AT5G06080.1 35.238 105 67 1 8 111 7 111 1.70e-15 72.8
Msa1255810 AT1G06280.1 36.709 79 50 0 7 85 23 101 2.21e-13 67.8
Msa1255810 AT2G19820.1 28.571 98 64 2 8 104 12 104 4.88e-11 59.3

Find 92 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGTTCCTGTTCATGATTC+TGG 0.107641 8_2:-55058327 Msa1255810:CDS
CTGTTGATGCTCCTTCTTCT+AGG 0.171774 8_2:-55058269 Msa1255810:CDS
CTCGCCTTTCCTGATTGTTT+TGG 0.240206 8_2:-55058594 Msa1255810:CDS
ATGTTCCTGTTCATGATTCT+GGG 0.297022 8_2:-55058326 Msa1255810:CDS
GCGAAGGTACACTCGGTGTT+TGG 0.338449 8_2:-55058843 Msa1255810:CDS
GTTCATGATTCTGGGTTGGC+TGG 0.362539 8_2:-55058318 Msa1255810:CDS
AAACGCTCCGGGTTGTCCTT+TGG 0.364971 8_2:+55058867 None:intergenic
CTACCTTGAAGGTCATTTGC+TGG 0.366573 8_2:+55058699 None:intergenic
GACCTGAGGACTGAGGTATT+TGG 0.383512 8_2:+55058629 None:intergenic
AGCAACCAGGGTCGATTGCC+GGG 0.393568 8_2:-55058213 Msa1255810:CDS
AAGCAACCAGGGTCGATTGC+CGG 0.397461 8_2:-55058214 Msa1255810:CDS
GGTGGTTCGGTCGGTGGCAG+TGG 0.408512 8_2:-55058378 Msa1255810:CDS
GGCTTAAAGCCAAAACAATC+AGG 0.409263 8_2:+55058585 None:intergenic
GAGGAACATGAGGAAGCATC+TGG 0.409895 8_2:+55058292 None:intergenic
CTAAAACAAAAGCTTCTTAG+TGG 0.418812 8_2:-55058459 Msa1255810:CDS
GCCAACCCATCATCACCATT+GGG 0.419815 8_2:+55058435 None:intergenic
TGTACCTTCGCGAAACGCTC+CGG 0.426352 8_2:+55058855 None:intergenic
TGAACAAGCCGTTGAATCCT+TGG 0.427880 8_2:-55058772 Msa1255810:CDS
CAGGAACATCAGCACCCATC+TGG 0.437219 8_2:+55058340 None:intergenic
GGTCGGTGGCAGTGGTGGCT+CGG 0.445795 8_2:-55058370 Msa1255810:CDS
TCCTGTTCATGATTCTGGGT+TGG 0.449021 8_2:-55058322 Msa1255810:CDS
CTTCATTCAACCTACCTTGA+AGG 0.452245 8_2:+55058688 None:intergenic
AGCTTGGGTGGCACTTCCTA+TGG 0.453993 8_2:+55058910 None:intergenic
GGGTTGTCCTTTGGAAAGTA+AGG 0.456967 8_2:+55058876 None:intergenic
TGTTGATGCTCCTTCTTCTA+GGG 0.467653 8_2:-55058268 Msa1255810:CDS
CTAGAAGAAGGAGCATCAAC+AGG 0.469028 8_2:+55058270 None:intergenic
CAGGAACATTAACATCCATC+TGG 0.471455 8_2:+55058406 None:intergenic
TGTTCCTGTTGGTGGTGGTT+CGG 0.474185 8_2:-55058391 Msa1255810:CDS
AGTGGCCCCAATGGTGATGA+TGG 0.478656 8_2:-55058441 Msa1255810:CDS
TGCCAACCCATCATCACCAT+TGG 0.483147 8_2:+55058434 None:intergenic
GCCAACCCAGAATCATGAAC+AGG 0.484253 8_2:+55058321 None:intergenic
GACCCTATCCATGGCTGTGT+GGG 0.485867 8_2:-55058723 Msa1255810:CDS
ATAGTCGGTGGAGAAGCATT+TGG 0.487182 8_2:+55058564 None:intergenic
ATGAGGCTGATGCCTGGTTG+AGG 0.492786 8_2:-55058746 Msa1255810:CDS
GGTTCGGTCGGTGGCAGTGG+TGG 0.492839 8_2:-55058375 Msa1255810:CDS
AATGGTGATGATGGGTTGGC+AGG 0.493280 8_2:-55058432 Msa1255810:CDS
CCGACCGAACCACCACCAAC+AGG 0.493564 8_2:+55058387 None:intergenic
TGGTGTATGAGGCTGATGCC+TGG 0.495597 8_2:-55058752 Msa1255810:CDS
CCTGTTGGTGGTGGTTCGGT+CGG 0.503841 8_2:-55058387 Msa1255810:CDS
GTTAATGTTCCTGTTGGTGG+TGG 0.504572 8_2:-55058396 Msa1255810:CDS
TAAGGAGCTAAGACACAGCT+TGG 0.506248 8_2:+55058894 None:intergenic
GATGTTAATGTTCCTGTTGG+TGG 0.510237 8_2:-55058399 Msa1255810:CDS
CGTTGAATCCTTGGTGTATG+AGG 0.513459 8_2:-55058763 Msa1255810:CDS
TAGGTTGAATGAAGCTCAAA+CGG 0.515884 8_2:-55058679 Msa1255810:CDS
GGCCAAATACCTCAGTCCTC+AGG 0.517957 8_2:-55058631 Msa1255810:CDS
GGACCCACACAGCCATGGAT+AGG 0.520313 8_2:+55058720 None:intergenic
GGCAGCTGATTAAAACTCAA+TGG 0.520686 8_2:+55058525 None:intergenic
CCCCAATGGTGATGATGGGT+TGG 0.523556 8_2:-55058436 Msa1255810:CDS
AAAGTATCAGCCCTAGAAGA+AGG 0.527095 8_2:+55058258 None:intergenic
TGAGGCTGATGCCTGGTTGA+GGG 0.528952 8_2:-55058745 Msa1255810:CDS
CTTAGCTCCTTACTTTCCAA+AGG 0.539710 8_2:-55058883 Msa1255810:CDS
GTTGGTGGTGGTTCGGTCGG+TGG 0.545494 8_2:-55058384 Msa1255810:CDS
TGGTTGAGGGACCCTATCCA+TGG 0.545516 8_2:-55058732 Msa1255810:CDS
GGTCCAGCAAATGACCTTCA+AGG 0.545737 8_2:-55058702 Msa1255810:CDS
CGCCGCAAGCTCAAACGTTG+TGG 0.546240 8_2:+55058187 None:intergenic
TGGCTCGGCAAGATTCCAGA+TGG 0.560308 8_2:-55058355 Msa1255810:CDS
AGATGCTTGCAAGCTGCACA+TGG 0.564745 8_2:+55058933 None:intergenic
TAGCACTAGCACAAGCAACC+AGG 0.569645 8_2:-55058226 Msa1255810:CDS
GGCTCGGCAAGATTCCAGAT+GGG 0.571382 8_2:-55058354 Msa1255810:CDS
AAGCTTCTTAGTGGCCCCAA+TGG 0.572030 8_2:-55058450 Msa1255810:CDS
CAAGAGTGTGAAGAACGAAC+TGG 0.572801 8_2:-55058652 Msa1255810:CDS
ATGGATAGGGTCCCTCAACC+AGG 0.580885 8_2:+55058734 None:intergenic
CTCATACACCAAGGATTCAA+CGG 0.581210 8_2:+55058764 None:intergenic
GAGCTAAGACACAGCTTGGG+TGG 0.584647 8_2:+55058898 None:intergenic
GACCCACACAGCCATGGATA+GGG 0.586069 8_2:+55058721 None:intergenic
CAGCTTGCAAGCATCTCCAT+AGG 0.587034 8_2:-55058926 Msa1255810:CDS
AAGGAGCTAAGACACAGCTT+GGG 0.589356 8_2:+55058895 None:intergenic
ATGGATGTTAATGTTCCTGT+TGG 0.590588 8_2:-55058402 Msa1255810:CDS
GTGGCCCCAATGGTGATGAT+GGG 0.592383 8_2:-55058440 Msa1255810:CDS
TTGCTGGACCCACACAGCCA+TGG 0.594631 8_2:+55058715 None:intergenic
TGAGTCGTTAACTCAGCAGC+TGG 0.600497 8_2:+55058504 None:intergenic
GCAACCAGGGTCGATTGCCG+GGG 0.604916 8_2:-55058212 Msa1255810:CDS
CCTCCACCGACTGTCATAGT+CGG 0.607543 8_2:+55058549 None:intergenic
GGACCCTATCCATGGCTGTG+TGG 0.612567 8_2:-55058724 Msa1255810:CDS
GCGTTTCGCGAAGGTACACT+CGG 0.621744 8_2:-55058850 Msa1255810:CDS
GCATCAACAGGAGGAACATG+AGG 0.622008 8_2:+55058282 None:intergenic
AGGAACATGAGGAAGCATCT+GGG 0.622177 8_2:+55058293 None:intergenic
AAAGCCAAAACAATCAGGAA+AGG 0.627370 8_2:+55058590 None:intergenic
CAGCAAATGACCTTCAAGGT+AGG 0.629800 8_2:-55058698 Msa1255810:CDS
TTACTTTCCAAAGGACAACC+CGG 0.635497 8_2:-55058874 Msa1255810:CDS
CCGACTATGACAGTCGGTGG+AGG 0.638161 8_2:-55058549 Msa1255810:CDS
CAACCCGGAGCGTTTCGCGA+AGG 0.646127 8_2:-55058859 Msa1255810:CDS
CCACCGACTATGACAGTCGG+TGG 0.655151 8_2:-55058552 Msa1255810:CDS
GAAGAAGGAGCATCAACAGG+AGG 0.655383 8_2:+55058273 None:intergenic
GATGCTTGCAAGCTGCACAT+GGG 0.660822 8_2:+55058934 None:intergenic
AGCACTAGCACAAGCAACCA+GGG 0.667332 8_2:-55058225 Msa1255810:CDS
AGAACAAATTCAACGACCTG+AGG 0.670458 8_2:+55058615 None:intergenic
TCTCCACCGACTATGACAGT+CGG 0.670674 8_2:-55058555 Msa1255810:CDS
TGGGTTGGCAGGATTCCAGA+TGG 0.675884 8_2:-55058421 Msa1255810:CDS
CCAACCCATCATCACCATTG+GGG 0.678760 8_2:+55058436 None:intergenic
GGCATCAGCCTCATACACCA+AGG 0.703811 8_2:+55058755 None:intergenic
ATTCAACGACCTGAGGACTG+AGG 0.713865 8_2:+55058622 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GACTAATCTGTTGAAAAAAC+TGG - chr8_2:55058343-55058362 Msa1255810:CDS 30.0%
!!! GCTTTTGTTTTAGAACTTCT+AGG + chr8_2:55058685-55058704 None:intergenic 30.0%
CTAAAACAAAAGCTTCTTAG+TGG - chr8_2:55058692-55058711 Msa1255810:CDS 30.0%
GACTAATCTGTTGAAAAAAC+TGG - chr8_2:55058343-55058362 Msa1255810:CDS 30.0%
!!! GCTTTTGTTTTAGAACTTCT+AGG + chr8_2:55058685-55058704 None:intergenic 30.0%
CTAAAACAAAAGCTTCTTAG+TGG - chr8_2:55058692-55058711 Msa1255810:CDS 30.0%
! TAGGTTGAATGAAGCTCAAA+CGG - chr8_2:55058472-55058491 Msa1255810:CDS 35.0%
AAAGCCAAAACAATCAGGAA+AGG + chr8_2:55058564-55058583 None:intergenic 35.0%
ATGGATGTTAATGTTCCTGT+TGG - chr8_2:55058749-55058768 Msa1255810:CDS 35.0%
ATGTTCCTGTTCATGATTCT+GGG - chr8_2:55058825-55058844 Msa1255810:CDS 35.0%
! TAGGTTGAATGAAGCTCAAA+CGG - chr8_2:55058472-55058491 Msa1255810:CDS 35.0%
AAAGCCAAAACAATCAGGAA+AGG + chr8_2:55058564-55058583 None:intergenic 35.0%
ATGGATGTTAATGTTCCTGT+TGG - chr8_2:55058749-55058768 Msa1255810:CDS 35.0%
ATGTTCCTGTTCATGATTCT+GGG - chr8_2:55058825-55058844 Msa1255810:CDS 35.0%
CTTAGCTCCTTACTTTCCAA+AGG - chr8_2:55058268-55058287 Msa1255810:CDS 40.0%
TTACTTTCCAAAGGACAACC+CGG - chr8_2:55058277-55058296 Msa1255810:CDS 40.0%
CTCATACACCAAGGATTCAA+CGG + chr8_2:55058390-55058409 None:intergenic 40.0%
CTTCATTCAACCTACCTTGA+AGG + chr8_2:55058466-55058485 None:intergenic 40.0%
AGAACAAATTCAACGACCTG+AGG + chr8_2:55058539-55058558 None:intergenic 40.0%
GGCTTAAAGCCAAAACAATC+AGG + chr8_2:55058569-55058588 None:intergenic 40.0%
GGCAGCTGATTAAAACTCAA+TGG + chr8_2:55058629-55058648 None:intergenic 40.0%
CAGGAACATTAACATCCATC+TGG + chr8_2:55058748-55058767 None:intergenic 40.0%
GATGTTAATGTTCCTGTTGG+TGG - chr8_2:55058752-55058771 Msa1255810:CDS 40.0%
GATGTTCCTGTTCATGATTC+TGG - chr8_2:55058824-55058843 Msa1255810:CDS 40.0%
TGTTGATGCTCCTTCTTCTA+GGG - chr8_2:55058883-55058902 Msa1255810:CDS 40.0%
! AAAGTATCAGCCCTAGAAGA+AGG + chr8_2:55058896-55058915 None:intergenic 40.0%
! AGGGCTGATACTTTTGACAA+TGG - chr8_2:55058902-55058921 Msa1255810:CDS 40.0%
CTTAGCTCCTTACTTTCCAA+AGG - chr8_2:55058268-55058287 Msa1255810:CDS 40.0%
TTACTTTCCAAAGGACAACC+CGG - chr8_2:55058277-55058296 Msa1255810:CDS 40.0%
CTCATACACCAAGGATTCAA+CGG + chr8_2:55058390-55058409 None:intergenic 40.0%
CTTCATTCAACCTACCTTGA+AGG + chr8_2:55058466-55058485 None:intergenic 40.0%
AGAACAAATTCAACGACCTG+AGG + chr8_2:55058539-55058558 None:intergenic 40.0%
GGCTTAAAGCCAAAACAATC+AGG + chr8_2:55058569-55058588 None:intergenic 40.0%
GGCAGCTGATTAAAACTCAA+TGG + chr8_2:55058629-55058648 None:intergenic 40.0%
CAGGAACATTAACATCCATC+TGG + chr8_2:55058748-55058767 None:intergenic 40.0%
GATGTTAATGTTCCTGTTGG+TGG - chr8_2:55058752-55058771 Msa1255810:CDS 40.0%
GATGTTCCTGTTCATGATTC+TGG - chr8_2:55058824-55058843 Msa1255810:CDS 40.0%
TGTTGATGCTCCTTCTTCTA+GGG - chr8_2:55058883-55058902 Msa1255810:CDS 40.0%
! AAAGTATCAGCCCTAGAAGA+AGG + chr8_2:55058896-55058915 None:intergenic 40.0%
! AGGGCTGATACTTTTGACAA+TGG - chr8_2:55058902-55058921 Msa1255810:CDS 40.0%
AAGGAGCTAAGACACAGCTT+GGG + chr8_2:55058259-55058278 None:intergenic 45.0%
TAAGGAGCTAAGACACAGCT+TGG + chr8_2:55058260-55058279 None:intergenic 45.0%
! GGGTTGTCCTTTGGAAAGTA+AGG + chr8_2:55058278-55058297 None:intergenic 45.0%
TGAACAAGCCGTTGAATCCT+TGG - chr8_2:55058379-55058398 Msa1255810:CDS 45.0%
! CGTTGAATCCTTGGTGTATG+AGG - chr8_2:55058388-55058407 Msa1255810:CDS 45.0%
CTACCTTGAAGGTCATTTGC+TGG + chr8_2:55058455-55058474 None:intergenic 45.0%
CAGCAAATGACCTTCAAGGT+AGG - chr8_2:55058453-55058472 Msa1255810:CDS 45.0%
! CAAGAGTGTGAAGAACGAAC+TGG - chr8_2:55058499-55058518 Msa1255810:CDS 45.0%
! CTCGCCTTTCCTGATTGTTT+TGG - chr8_2:55058557-55058576 Msa1255810:CDS 45.0%
!! ATAGTCGGTGGAGAAGCATT+TGG + chr8_2:55058590-55058609 None:intergenic 45.0%
! GTTAATGTTCCTGTTGGTGG+TGG - chr8_2:55058755-55058774 Msa1255810:CDS 45.0%
TCCTGTTCATGATTCTGGGT+TGG - chr8_2:55058829-55058848 Msa1255810:CDS 45.0%
!! AGGAACATGAGGAAGCATCT+GGG + chr8_2:55058861-55058880 None:intergenic 45.0%
!! CTAGAAGAAGGAGCATCAAC+AGG + chr8_2:55058884-55058903 None:intergenic 45.0%
CTGTTGATGCTCCTTCTTCT+AGG - chr8_2:55058882-55058901 Msa1255810:CDS 45.0%
AAGGAGCTAAGACACAGCTT+GGG + chr8_2:55058259-55058278 None:intergenic 45.0%
TAAGGAGCTAAGACACAGCT+TGG + chr8_2:55058260-55058279 None:intergenic 45.0%
! GGGTTGTCCTTTGGAAAGTA+AGG + chr8_2:55058278-55058297 None:intergenic 45.0%
TGAACAAGCCGTTGAATCCT+TGG - chr8_2:55058379-55058398 Msa1255810:CDS 45.0%
! CGTTGAATCCTTGGTGTATG+AGG - chr8_2:55058388-55058407 Msa1255810:CDS 45.0%
CTACCTTGAAGGTCATTTGC+TGG + chr8_2:55058455-55058474 None:intergenic 45.0%
CAGCAAATGACCTTCAAGGT+AGG - chr8_2:55058453-55058472 Msa1255810:CDS 45.0%
! CAAGAGTGTGAAGAACGAAC+TGG - chr8_2:55058499-55058518 Msa1255810:CDS 45.0%
! CTCGCCTTTCCTGATTGTTT+TGG - chr8_2:55058557-55058576 Msa1255810:CDS 45.0%
!! ATAGTCGGTGGAGAAGCATT+TGG + chr8_2:55058590-55058609 None:intergenic 45.0%
! GTTAATGTTCCTGTTGGTGG+TGG - chr8_2:55058755-55058774 Msa1255810:CDS 45.0%
TCCTGTTCATGATTCTGGGT+TGG - chr8_2:55058829-55058848 Msa1255810:CDS 45.0%
!! AGGAACATGAGGAAGCATCT+GGG + chr8_2:55058861-55058880 None:intergenic 45.0%
!! CTAGAAGAAGGAGCATCAAC+AGG + chr8_2:55058884-55058903 None:intergenic 45.0%
CTGTTGATGCTCCTTCTTCT+AGG - chr8_2:55058882-55058901 Msa1255810:CDS 45.0%
GATGCTTGCAAGCTGCACAT+GGG + chr8_2:55058220-55058239 None:intergenic 50.0%
AGATGCTTGCAAGCTGCACA+TGG + chr8_2:55058221-55058240 None:intergenic 50.0%
CAGCTTGCAAGCATCTCCAT+AGG - chr8_2:55058225-55058244 Msa1255810:CDS 50.0%
GGTCCAGCAAATGACCTTCA+AGG - chr8_2:55058449-55058468 Msa1255810:CDS 50.0%
! GACCTGAGGACTGAGGTATT+TGG + chr8_2:55058525-55058544 None:intergenic 50.0%
ATTCAACGACCTGAGGACTG+AGG + chr8_2:55058532-55058551 None:intergenic 50.0%
! TCTCCACCGACTATGACAGT+CGG - chr8_2:55058596-55058615 Msa1255810:CDS 50.0%
TGAGTCGTTAACTCAGCAGC+TGG + chr8_2:55058650-55058669 None:intergenic 50.0%
AAGCTTCTTAGTGGCCCCAA+TGG - chr8_2:55058701-55058720 Msa1255810:CDS 50.0%
CCAACCCATCATCACCATTG+GGG + chr8_2:55058718-55058737 None:intergenic 50.0%
GCCAACCCATCATCACCATT+GGG + chr8_2:55058719-55058738 None:intergenic 50.0%
TGCCAACCCATCATCACCAT+TGG + chr8_2:55058720-55058739 None:intergenic 50.0%
!! AATGGTGATGATGGGTTGGC+AGG - chr8_2:55058719-55058738 Msa1255810:CDS 50.0%
!! TGTTCCTGTTGGTGGTGGTT+CGG - chr8_2:55058760-55058779 Msa1255810:CDS 50.0%
GCCAACCCAGAATCATGAAC+AGG + chr8_2:55058833-55058852 None:intergenic 50.0%
!! GTTCATGATTCTGGGTTGGC+TGG - chr8_2:55058833-55058852 Msa1255810:CDS 50.0%
!! GAGGAACATGAGGAAGCATC+TGG + chr8_2:55058862-55058881 None:intergenic 50.0%
GCATCAACAGGAGGAACATG+AGG + chr8_2:55058872-55058891 None:intergenic 50.0%
!! GAAGAAGGAGCATCAACAGG+AGG + chr8_2:55058881-55058900 None:intergenic 50.0%
TAGCACTAGCACAAGCAACC+AGG - chr8_2:55058925-55058944 Msa1255810:CDS 50.0%
AGCACTAGCACAAGCAACCA+GGG - chr8_2:55058926-55058945 Msa1255810:CDS 50.0%
GATGCTTGCAAGCTGCACAT+GGG + chr8_2:55058220-55058239 None:intergenic 50.0%
AGATGCTTGCAAGCTGCACA+TGG + chr8_2:55058221-55058240 None:intergenic 50.0%
CAGCTTGCAAGCATCTCCAT+AGG - chr8_2:55058225-55058244 Msa1255810:CDS 50.0%
GGTCCAGCAAATGACCTTCA+AGG - chr8_2:55058449-55058468 Msa1255810:CDS 50.0%
! GACCTGAGGACTGAGGTATT+TGG + chr8_2:55058525-55058544 None:intergenic 50.0%
ATTCAACGACCTGAGGACTG+AGG + chr8_2:55058532-55058551 None:intergenic 50.0%
! TCTCCACCGACTATGACAGT+CGG - chr8_2:55058596-55058615 Msa1255810:CDS 50.0%
TGAGTCGTTAACTCAGCAGC+TGG + chr8_2:55058650-55058669 None:intergenic 50.0%
AAGCTTCTTAGTGGCCCCAA+TGG - chr8_2:55058701-55058720 Msa1255810:CDS 50.0%
CCAACCCATCATCACCATTG+GGG + chr8_2:55058718-55058737 None:intergenic 50.0%
GCCAACCCATCATCACCATT+GGG + chr8_2:55058719-55058738 None:intergenic 50.0%
TGCCAACCCATCATCACCAT+TGG + chr8_2:55058720-55058739 None:intergenic 50.0%
!! AATGGTGATGATGGGTTGGC+AGG - chr8_2:55058719-55058738 Msa1255810:CDS 50.0%
!! TGTTCCTGTTGGTGGTGGTT+CGG - chr8_2:55058760-55058779 Msa1255810:CDS 50.0%
GCCAACCCAGAATCATGAAC+AGG + chr8_2:55058833-55058852 None:intergenic 50.0%
!! GTTCATGATTCTGGGTTGGC+TGG - chr8_2:55058833-55058852 Msa1255810:CDS 50.0%
!! GAGGAACATGAGGAAGCATC+TGG + chr8_2:55058862-55058881 None:intergenic 50.0%
GCATCAACAGGAGGAACATG+AGG + chr8_2:55058872-55058891 None:intergenic 50.0%
!! GAAGAAGGAGCATCAACAGG+AGG + chr8_2:55058881-55058900 None:intergenic 50.0%
TAGCACTAGCACAAGCAACC+AGG - chr8_2:55058925-55058944 Msa1255810:CDS 50.0%
AGCACTAGCACAAGCAACCA+GGG - chr8_2:55058926-55058945 Msa1255810:CDS 50.0%
AGCTTGGGTGGCACTTCCTA+TGG + chr8_2:55058244-55058263 None:intergenic 55.0%
GAGCTAAGACACAGCTTGGG+TGG + chr8_2:55058256-55058275 None:intergenic 55.0%
AAACGCTCCGGGTTGTCCTT+TGG + chr8_2:55058287-55058306 None:intergenic 55.0%
TGTACCTTCGCGAAACGCTC+CGG + chr8_2:55058299-55058318 None:intergenic 55.0%
! GCGTTTCGCGAAGGTACACT+CGG - chr8_2:55058301-55058320 Msa1255810:CDS 55.0%
!! GCGAAGGTACACTCGGTGTT+TGG - chr8_2:55058308-55058327 Msa1255810:CDS 55.0%
GGCATCAGCCTCATACACCA+AGG + chr8_2:55058399-55058418 None:intergenic 55.0%
TGGTGTATGAGGCTGATGCC+TGG - chr8_2:55058399-55058418 Msa1255810:CDS 55.0%
ATGAGGCTGATGCCTGGTTG+AGG - chr8_2:55058405-55058424 Msa1255810:CDS 55.0%
TGAGGCTGATGCCTGGTTGA+GGG - chr8_2:55058406-55058425 Msa1255810:CDS 55.0%
ATGGATAGGGTCCCTCAACC+AGG + chr8_2:55058420-55058439 None:intergenic 55.0%
TGGTTGAGGGACCCTATCCA+TGG - chr8_2:55058419-55058438 Msa1255810:CDS 55.0%
GACCCTATCCATGGCTGTGT+GGG - chr8_2:55058428-55058447 Msa1255810:CDS 55.0%
GACCCACACAGCCATGGATA+GGG + chr8_2:55058433-55058452 None:intergenic 55.0%
GGCCAAATACCTCAGTCCTC+AGG - chr8_2:55058520-55058539 Msa1255810:CDS 55.0%
! CCTCCACCGACTGTCATAGT+CGG + chr8_2:55058605-55058624 None:intergenic 55.0%
! AGTGGCCCCAATGGTGATGA+TGG - chr8_2:55058710-55058729 Msa1255810:CDS 55.0%
! GTGGCCCCAATGGTGATGAT+GGG - chr8_2:55058711-55058730 Msa1255810:CDS 55.0%
!! CCCCAATGGTGATGATGGGT+TGG - chr8_2:55058715-55058734 Msa1255810:CDS 55.0%
!! TGGGTTGGCAGGATTCCAGA+TGG - chr8_2:55058730-55058749 Msa1255810:CDS 55.0%
! TGGCTCGGCAAGATTCCAGA+TGG - chr8_2:55058796-55058815 Msa1255810:CDS 55.0%
GGCTCGGCAAGATTCCAGAT+GGG - chr8_2:55058797-55058816 Msa1255810:CDS 55.0%
CAGGAACATCAGCACCCATC+TGG + chr8_2:55058814-55058833 None:intergenic 55.0%
AAGCAACCAGGGTCGATTGC+CGG - chr8_2:55058937-55058956 Msa1255810:CDS 55.0%
AGCTTGGGTGGCACTTCCTA+TGG + chr8_2:55058244-55058263 None:intergenic 55.0%
GAGCTAAGACACAGCTTGGG+TGG + chr8_2:55058256-55058275 None:intergenic 55.0%
AAACGCTCCGGGTTGTCCTT+TGG + chr8_2:55058287-55058306 None:intergenic 55.0%
TGTACCTTCGCGAAACGCTC+CGG + chr8_2:55058299-55058318 None:intergenic 55.0%
! GCGTTTCGCGAAGGTACACT+CGG - chr8_2:55058301-55058320 Msa1255810:CDS 55.0%
!! GCGAAGGTACACTCGGTGTT+TGG - chr8_2:55058308-55058327 Msa1255810:CDS 55.0%
GGCATCAGCCTCATACACCA+AGG + chr8_2:55058399-55058418 None:intergenic 55.0%
TGGTGTATGAGGCTGATGCC+TGG - chr8_2:55058399-55058418 Msa1255810:CDS 55.0%
ATGAGGCTGATGCCTGGTTG+AGG - chr8_2:55058405-55058424 Msa1255810:CDS 55.0%
TGAGGCTGATGCCTGGTTGA+GGG - chr8_2:55058406-55058425 Msa1255810:CDS 55.0%
ATGGATAGGGTCCCTCAACC+AGG + chr8_2:55058420-55058439 None:intergenic 55.0%
TGGTTGAGGGACCCTATCCA+TGG - chr8_2:55058419-55058438 Msa1255810:CDS 55.0%
GACCCTATCCATGGCTGTGT+GGG - chr8_2:55058428-55058447 Msa1255810:CDS 55.0%
GACCCACACAGCCATGGATA+GGG + chr8_2:55058433-55058452 None:intergenic 55.0%
GGCCAAATACCTCAGTCCTC+AGG - chr8_2:55058520-55058539 Msa1255810:CDS 55.0%
! CCTCCACCGACTGTCATAGT+CGG + chr8_2:55058605-55058624 None:intergenic 55.0%
! AGTGGCCCCAATGGTGATGA+TGG - chr8_2:55058710-55058729 Msa1255810:CDS 55.0%
! GTGGCCCCAATGGTGATGAT+GGG - chr8_2:55058711-55058730 Msa1255810:CDS 55.0%
!! CCCCAATGGTGATGATGGGT+TGG - chr8_2:55058715-55058734 Msa1255810:CDS 55.0%
!! TGGGTTGGCAGGATTCCAGA+TGG - chr8_2:55058730-55058749 Msa1255810:CDS 55.0%
! TGGCTCGGCAAGATTCCAGA+TGG - chr8_2:55058796-55058815 Msa1255810:CDS 55.0%
GGCTCGGCAAGATTCCAGAT+GGG - chr8_2:55058797-55058816 Msa1255810:CDS 55.0%
CAGGAACATCAGCACCCATC+TGG + chr8_2:55058814-55058833 None:intergenic 55.0%
AAGCAACCAGGGTCGATTGC+CGG - chr8_2:55058937-55058956 Msa1255810:CDS 55.0%
GTACCTTCGCGAAACGCTCC+GGG + chr8_2:55058298-55058317 None:intergenic 60.0%
GGACCCTATCCATGGCTGTG+TGG - chr8_2:55058427-55058446 Msa1255810:CDS 60.0%
GGACCCACACAGCCATGGAT+AGG + chr8_2:55058434-55058453 None:intergenic 60.0%
TTGCTGGACCCACACAGCCA+TGG + chr8_2:55058439-55058458 None:intergenic 60.0%
! CCACCGACTGTCATAGTCGG+TGG + chr8_2:55058602-55058621 None:intergenic 60.0%
! CCACCGACTATGACAGTCGG+TGG - chr8_2:55058599-55058618 Msa1255810:CDS 60.0%
CCGACTATGACAGTCGGTGG+AGG - chr8_2:55058602-55058621 Msa1255810:CDS 60.0%
!! CCTGTTGGTGGTGGTTCGGT+CGG - chr8_2:55058764-55058783 Msa1255810:CDS 60.0%
AGCAACCAGGGTCGATTGCC+GGG - chr8_2:55058938-55058957 Msa1255810:CDS 60.0%
GTACCTTCGCGAAACGCTCC+GGG + chr8_2:55058298-55058317 None:intergenic 60.0%
GGACCCTATCCATGGCTGTG+TGG - chr8_2:55058427-55058446 Msa1255810:CDS 60.0%
GGACCCACACAGCCATGGAT+AGG + chr8_2:55058434-55058453 None:intergenic 60.0%
TTGCTGGACCCACACAGCCA+TGG + chr8_2:55058439-55058458 None:intergenic 60.0%
! CCACCGACTGTCATAGTCGG+TGG + chr8_2:55058602-55058621 None:intergenic 60.0%
! CCACCGACTATGACAGTCGG+TGG - chr8_2:55058599-55058618 Msa1255810:CDS 60.0%
CCGACTATGACAGTCGGTGG+AGG - chr8_2:55058602-55058621 Msa1255810:CDS 60.0%
!! CCTGTTGGTGGTGGTTCGGT+CGG - chr8_2:55058764-55058783 Msa1255810:CDS 60.0%
AGCAACCAGGGTCGATTGCC+GGG - chr8_2:55058938-55058957 Msa1255810:CDS 60.0%
! CAACCCGGAGCGTTTCGCGA+AGG - chr8_2:55058292-55058311 Msa1255810:CDS 65.0%
CCGACCGAACCACCACCAAC+AGG + chr8_2:55058767-55058786 None:intergenic 65.0%
!! GTTGGTGGTGGTTCGGTCGG+TGG - chr8_2:55058767-55058786 Msa1255810:CDS 65.0%
GCAACCAGGGTCGATTGCCG+GGG - chr8_2:55058939-55058958 Msa1255810:CDS 65.0%
! CAACCCGGAGCGTTTCGCGA+AGG - chr8_2:55058292-55058311 Msa1255810:CDS 65.0%
CCGACCGAACCACCACCAAC+AGG + chr8_2:55058767-55058786 None:intergenic 65.0%
!! GTTGGTGGTGGTTCGGTCGG+TGG - chr8_2:55058767-55058786 Msa1255810:CDS 65.0%
GCAACCAGGGTCGATTGCCG+GGG - chr8_2:55058939-55058958 Msa1255810:CDS 65.0%
!! GGTGGTTCGGTCGGTGGCAG+TGG - chr8_2:55058773-55058792 Msa1255810:CDS 70.0%
! GGTTCGGTCGGTGGCAGTGG+TGG - chr8_2:55058776-55058795 Msa1255810:CDS 70.0%
GGTCGGTGGCAGTGGTGGCT+CGG - chr8_2:55058781-55058800 Msa1255810:CDS 70.0%
!! GGTGGTTCGGTCGGTGGCAG+TGG - chr8_2:55058773-55058792 Msa1255810:CDS 70.0%
! GGTTCGGTCGGTGGCAGTGG+TGG - chr8_2:55058776-55058795 Msa1255810:CDS 70.0%
GGTCGGTGGCAGTGGTGGCT+CGG - chr8_2:55058781-55058800 Msa1255810:CDS 70.0%
GGCTCCCCGGCAATCGACCC+TGG + chr8_2:55058946-55058965 None:intergenic 75.0%
GGCTCCCCGGCAATCGACCC+TGG + chr8_2:55058946-55058965 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 55058197 55058976 55058197 ID=Msa1255810;Name=Msa1255810
chr8_2 mRNA 55058197 55058976 55058197 ID=Msa1255810-mRNA-1;Parent=Msa1255810;Name=Msa1255810-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|259
chr8_2 exon 55058197 55058976 55058197 ID=Msa1255810-mRNA-1:exon:16995;Parent=Msa1255810-mRNA-1
chr8_2 CDS 55058197 55058976 55058197 ID=Msa1255810-mRNA-1:cds;Parent=Msa1255810-mRNA-1
Gene Sequence

>Msa1255810

ATGGATAGAGAGATTATTCGCCCATGTGCAGCTTGCAAGCATCTCCATAGGAAGTGCCACCCAAGCTGTGTCTTAGCTCCTTACTTTCCAAAGGACAACCCGGAGCGTTTCGCGAAGGTACACTCGGTGTTTGGAAAGAGAAATGTGACTAATCTGTTGAAAAAACTGGATGTTTCACATCATGAACAAGCCGTTGAATCCTTGGTGTATGAGGCTGATGCCTGGTTGAGGGACCCTATCCATGGCTGTGTGGGTCCAGCAAATGACCTTCAAGGTAGGTTGAATGAAGCTCAAACGGAGCTCAAGAGTGTGAAGAACGAACTGGCCAAATACCTCAGTCCTCAGGTCGTTGAATTTGTTCTCGCCTTTCCTGATTGTTTTGGCTTTAAGCCAAATGCTTCTCCACCGACTATGACAGTCGGTGGAGGACCATTGAGTTTTAATCAGCTGCCAGCTGCTGAGTTAACGACTCAGCAGCTTCACAACCTAGAAGTTCTAAAACAAAAGCTTCTTAGTGGCCCCAATGGTGATGATGGGTTGGCAGGATTCCAGATGGATGTTAATGTTCCTGTTGGTGGTGGTTCGGTCGGTGGCAGTGGTGGCTCGGCAAGATTCCAGATGGGTGCTGATGTTCCTGTTCATGATTCTGGGTTGGCTGGATCCCAGATGCTTCCTCATGTTCCTCCTGTTGATGCTCCTTCTTCTAGGGCTGATACTTTTGACAATGGTAGCACTAGCACAAGCAACCAGGGTCGATTGCCGGGGAGCCACAACGTTTGA
Protein sequence

>Msa1255810

MDREIIRPCAACKHLHRKCHPSCVLAPYFPKDNPERFAKVHSVFGKRNVTNLLKKLDVSHHEQAVESLVYEADAWLRDPIHGCVGPANDLQGRLNEAQTELKSVKNELAKYLSPQVVEFVLAFPDCFGFKPNASPPTMTVGGGPLSFNQLPAAELTTQQLHNLEVLKQKLLSGPNGDDGLAGFQMDVNVPVGGGSVGGSGGSARFQMGADVPVHDSGLAGSQMLPHVPPVDAPSSRADTFDNGSTSTSNQGRLPGSHNV*