AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1256530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1256530 MtrunA17_Chr4g0053901 96.802 469 4 2 1 458 1 469 0.0 936
Msa1256530 MtrunA17_Chr5g0401421 67.181 454 122 9 25 458 1 447 0.0 595
Msa1256530 MtrunA17_Chr2g0323111 46.569 204 90 1 255 458 123 307 8.17e-60 198
Msa1256530 MtrunA17_Chr2g0323111 37.255 153 80 3 6 153 4 145 1.98e-20 92.0
Msa1256530 MtrunA17_Chr5g0401131 28.710 411 249 8 59 458 114 491 1.93e-51 181
Msa1256530 MtrunA17_Chr2g0329591 28.979 421 238 12 58 458 73 452 7.94e-48 170
Msa1256530 MtrunA17_Chr3g0125811 27.316 421 259 9 43 456 189 569 3.35e-38 146
Msa1256530 MtrunA17_Chr1g0159461 26.601 406 255 7 57 456 223 591 4.72e-38 146
Msa1256530 MtrunA17_Chr5g0447691 26.098 410 254 8 56 456 174 543 4.47e-37 142
Msa1256530 MtrunA17_Chr2g0319091 25.325 462 289 11 2 456 166 578 5.72e-35 137
Msa1256530 MtrunA17_Chr8g0357601 26.039 457 285 13 8 456 140 551 7.87e-34 134
Msa1256530 MtrunA17_Chr3g0101141 23.971 413 274 9 47 456 166 541 1.55e-33 132
Msa1256530 MtrunA17_Chr4g0076091 26.139 417 248 10 56 457 172 543 1.65e-33 132
Msa1256530 MtrunA17_Chr5g0445331 25.369 406 266 8 56 456 158 531 3.30e-32 129
Msa1256530 MtrunA17_Chr8g0344101 28.140 430 238 14 52 456 216 599 6.70e-32 128
Msa1256530 MtrunA17_Chr7g0224271 25.362 414 252 9 59 456 300 672 4.05e-30 123
Msa1256530 MtrunA17_Chr3g0085681 23.898 431 273 10 60 456 26 435 1.42e-29 120
Msa1256530 MtrunA17_Chr2g0290241 24.939 409 261 9 56 456 54 424 1.51e-29 119
Msa1256530 MtrunA17_Chr2g0306641 24.880 418 246 15 58 459 124 489 1.65e-28 117
Msa1256530 MtrunA17_Chr4g0031711 23.928 443 288 12 27 459 332 735 4.21e-28 117
Msa1256530 MtrunA17_Chr3g0110971 24.940 417 243 11 60 456 220 586 9.40e-28 116
Msa1256530 MtrunA17_Chr4g0038851 24.380 484 291 14 22 456 11 468 3.48e-27 113
Msa1256530 MtrunA17_Chr4g0031731 24.574 411 265 14 56 457 251 625 8.21e-27 113
Msa1256530 MtrunA17_Chr4g0031741 26.225 408 253 12 59 458 284 651 1.26e-26 113
Msa1256530 MtrunA17_Chr4g0057891 26.950 423 234 15 58 456 148 519 3.37e-26 111
Msa1256530 MtrunA17_Chr4g0031701 24.390 410 267 13 56 457 251 625 3.76e-26 111
Msa1256530 MtrunA17_Chr2g0328391 23.659 410 271 10 56 458 296 670 5.88e-26 111
Msa1256530 MtrunA17_Chr4g0031721 24.515 412 265 12 56 459 313 686 1.05e-25 110
Msa1256530 MtrunA17_Chr5g0396491 24.078 461 286 11 39 456 38 477 1.17e-25 109
Msa1256530 MtrunA17_Chr8g0376381 23.881 402 251 10 60 456 170 521 1.45e-24 106
Msa1256530 MtrunA17_Chr7g0238891 23.601 411 270 9 56 459 369 742 2.21e-24 106
Msa1256530 MtrunA17_Chr2g0328341 23.415 410 270 10 56 459 269 640 4.78e-24 105
Msa1256530 MtrunA17_Chr2g0328381 23.349 424 281 10 41 458 257 642 5.03e-24 105
Msa1256530 MtrunA17_Chr6g0468561 26.506 415 248 12 55 456 291 661 1.61e-23 103
Msa1256530 MtrunA17_Chr3g0085361 24.638 345 215 9 144 456 18 349 7.83e-22 96.7
Msa1256530 MtrunA17_Chr2g0328411 22.829 403 270 8 59 457 289 654 1.56e-21 97.8
Msa1256530 MtrunA17_Chr4g0039661 25.899 417 245 16 54 456 188 554 3.53e-21 96.3
Msa1256530 MtrunA17_Chr4g0057291 22.087 412 271 12 59 459 292 664 6.01e-21 95.9
Msa1256530 MtrunA17_Chr2g0328431 21.411 411 270 8 59 459 289 656 1.72e-19 91.3
Msa1256530 MtrunA17_Chr1g0200401 24.476 429 243 17 59 456 80 458 5.44e-19 89.4
Msa1256530 MtrunA17_Chr7g0245601 23.515 404 251 14 62 458 441 793 1.44e-18 89.0
Msa1256530 MtrunA17_Chr7g0236851 24.051 316 195 6 152 456 375 656 7.12e-18 86.3
Msa1256530 MtrunA17_Chr2g0328401 22.667 375 246 8 59 428 290 625 3.96e-17 84.0
Msa1256530 MtrunA17_Chr5g0424131 25.301 415 258 16 58 456 120 498 9.28e-17 82.4
Msa1256530 MtrunA17_Chr4g0038761 22.660 406 256 14 62 458 87 443 2.10e-16 81.3
Msa1256530 MtrunA17_Chr3g0114841 23.373 415 268 14 58 456 119 499 1.63e-15 78.6
Msa1256530 MtrunA17_Chr2g0328361 20.388 412 255 10 56 459 271 617 2.42e-15 78.6
Msa1256530 MtrunA17_Chr8g0383981 22.195 410 258 14 59 458 139 497 2.23e-14 75.1
Msa1256530 MtrunA17_Chr4g0054071 34.211 114 66 3 57 168 131 237 1.27e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1256530 AT3G49950.1 55.682 440 141 11 36 459 9 410 8.87e-164 468
Msa1256530 AT5G67411.1 54.185 227 68 5 183 398 1 202 7.31e-70 221
Msa1256530 AT4G37650.1 29.496 417 249 8 57 458 144 530 2.93e-51 182
Msa1256530 AT1G50600.3 24.279 416 275 8 44 456 147 525 2.43e-36 140
Msa1256530 AT1G50600.2 24.279 416 275 8 44 456 222 600 3.42e-36 141
Msa1256530 AT1G50600.1 24.279 416 275 8 44 456 218 596 4.09e-36 141
Msa1256530 AT1G21450.1 25.366 410 256 8 57 456 223 592 4.36e-36 140
Msa1256530 AT2G04890.1 26.368 402 256 8 56 456 50 412 6.67e-36 137
Msa1256530 AT5G48150.1 26.290 407 250 10 60 456 123 489 2.92e-34 134
Msa1256530 AT5G48150.4 26.290 407 250 10 60 456 123 489 2.92e-34 134
Msa1256530 AT5G48150.2 26.290 407 250 10 60 456 123 489 2.92e-34 134
Msa1256530 AT5G48150.3 26.290 407 250 10 60 456 123 489 2.92e-34 134
Msa1256530 AT4G17230.1 26.406 409 258 8 56 458 155 526 7.24e-31 125
Msa1256530 AT4G17230.2 26.406 409 258 8 56 458 155 526 7.24e-31 125
Msa1256530 AT1G50420.1 25.850 441 265 11 59 456 57 478 2.32e-30 123
Msa1256530 AT5G17490.1 25.061 407 247 9 58 456 159 515 2.40e-29 120
Msa1256530 AT3G03450.1 25.672 409 244 11 60 456 184 544 1.51e-27 115
Msa1256530 AT2G29065.1 26.521 411 260 11 59 459 252 630 2.31e-27 115
Msa1256530 AT5G52510.1 22.967 418 264 8 55 456 264 639 6.09e-27 114
Msa1256530 AT3G46600.2 25.304 411 260 9 56 458 1 372 2.50e-26 110
Msa1256530 AT3G46600.1 25.243 412 261 9 56 459 209 581 1.11e-25 110
Msa1256530 AT3G46600.3 25.304 411 260 9 56 458 177 548 1.60e-25 109
Msa1256530 AT3G13840.1 26.634 413 241 16 57 456 146 509 2.35e-24 106
Msa1256530 AT2G01570.1 22.750 400 262 9 60 456 225 580 2.72e-24 106
Msa1256530 AT2G29060.1 24.228 421 266 12 52 459 314 694 5.36e-24 105
Msa1256530 AT2G29060.2 24.228 421 266 12 52 459 277 657 5.89e-24 105
Msa1256530 AT1G07530.1 23.040 421 285 10 43 458 380 766 1.64e-23 104
Msa1256530 AT5G59450.1 23.462 439 291 10 30 459 198 600 1.52e-22 101
Msa1256530 AT1G14920.1 23.250 400 260 9 60 456 173 528 1.99e-22 100
Msa1256530 AT2G37650.1 22.738 409 272 11 56 458 344 714 1.00e-21 99.0
Msa1256530 AT2G37650.2 22.738 409 272 11 56 458 332 702 1.11e-21 98.6
Msa1256530 AT3G54220.1 24.570 407 246 16 62 458 296 651 1.13e-19 92.4
Msa1256530 AT5G66770.1 24.267 375 233 11 58 425 219 549 1.49e-19 92.0
Msa1256530 AT1G63100.2 22.010 418 265 13 59 456 277 653 3.65e-17 84.7
Msa1256530 AT1G63100.1 22.010 418 265 13 59 456 252 628 4.11e-17 84.3
Msa1256530 AT5G41920.1 23.587 407 252 13 59 456 43 399 4.78e-17 83.2
Msa1256530 AT1G55580.1 21.591 440 270 12 56 456 41 444 5.60e-17 83.6
Msa1256530 AT4G08250.1 23.909 481 270 22 5 456 63 476 1.53e-13 72.8

Find 81 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGTTTGGTAGGCTTATC+TGG 0.192122 8_2:+56578284 None:intergenic
GATCTTTCTTATGAAGAATT+AGG 0.219298 8_2:-56577714 Msa1256530:CDS
TAACACCTTGGCATCGTTTC+GGG 0.237807 8_2:-56577941 Msa1256530:CDS
CTTTGTTCCCATTTGCCTAT+AGG 0.258304 8_2:+56577071 None:intergenic
GGTCTGCATTTAATTATCTA+TGG 0.266633 8_2:-56577226 Msa1256530:CDS
CATATGAGTTTGAATTTGAT+AGG 0.282770 8_2:+56577408 None:intergenic
CTTCTTTGATTGAACATTTG+AGG 0.307298 8_2:-56577508 Msa1256530:CDS
TACTTGATTGTTCTTGTTCA+TGG 0.313905 8_2:+56578310 None:intergenic
CACTTGCTCAACAAATCCTT+TGG 0.324306 8_2:-56578163 Msa1256530:CDS
CACGATTTGTGAGGGCTCTA+AGG 0.339226 8_2:+56578081 None:intergenic
TCCAAAGCTTTAACTACCTT+TGG 0.348338 8_2:-56578251 Msa1256530:CDS
TTGAAAAGGTTGTTGTTGTT+TGG 0.354768 8_2:+56578345 None:intergenic
CTATTACCACTACGTCTCTT+AGG 0.356293 8_2:-56577349 Msa1256530:CDS
TTCTTTGATTGAACATTTGA+GGG 0.357439 8_2:-56577507 Msa1256530:CDS
TTAACACCTTGGCATCGTTT+CGG 0.361682 8_2:-56577942 Msa1256530:CDS
TAGTTAAAGCTTTGGATGTT+GGG 0.379512 8_2:+56578258 None:intergenic
TGAGAATCTGTGTGTATCTA+TGG 0.382234 8_2:+56577992 None:intergenic
ATGAGCATATTGTGCTTACT+TGG 0.383561 8_2:-56576941 Msa1256530:CDS
ACAACTTGGTCAGTAGATTA+AGG 0.384086 8_2:-56577247 Msa1256530:CDS
AGCTGCTAATGCAGCAATTC+TGG 0.384262 8_2:-56577913 Msa1256530:CDS
GTTGTTGTTTGGTGCAAATA+AGG 0.386349 8_2:+56578356 None:intergenic
TCATAAGAAAGATCAAGTTT+AGG 0.388296 8_2:+56577723 None:intergenic
ACATTTGAGGGTTCAACGTT+TGG 0.395244 8_2:-56577495 Msa1256530:CDS
TTGTTTCTTAACGCGCTTCG+AGG 0.401307 8_2:-56577323 Msa1256530:CDS
ACCAAAGGTAGTTAAAGCTT+TGG 0.401348 8_2:+56578250 None:intergenic
GGTTTATTCAAGTGAAGCTT+TGG 0.412729 8_2:-56577474 Msa1256530:CDS
GAATTTGATAGGGTTTCATC+TGG 0.416336 8_2:+56577419 None:intergenic
AAATGTTGAACTAATTAAAC+AGG 0.426121 8_2:+56576882 None:intergenic
ACAAATCGTGCAGCGAAAAC+CGG 0.428781 8_2:-56578068 Msa1256530:CDS
GTAGTTAAAGCTTTGGATGT+TGG 0.435151 8_2:+56578257 None:intergenic
GGTATGAAGCAGATATTTGT+TGG 0.435178 8_2:-56577148 Msa1256530:CDS
ACTTGCTCAACAAATCCTTT+GGG 0.443862 8_2:-56578162 Msa1256530:CDS
GAGTTGAACCTATAGGCAAA+TGG 0.454475 8_2:-56577079 Msa1256530:CDS
TGATGAACATGCTGCTGGAT+GGG 0.466685 8_2:-56576979 Msa1256530:CDS
GTTGGGAACCATGGTTTGGT+AGG 0.471948 8_2:+56578275 None:intergenic
GGATGTTGGGAACCATGGTT+TGG 0.473234 8_2:+56578271 None:intergenic
CATTGAGCTAGCTAACTTCG+TGG 0.473787 8_2:-56577967 Msa1256530:CDS
TGAAGAATTAGGTTCTAAGT+TGG 0.481128 8_2:-56577703 Msa1256530:CDS
GATGAACATGCTGCTGGATG+GGG 0.481456 8_2:-56576978 Msa1256530:CDS
GTTGAGAGAGTTGAACCTAT+AGG 0.489058 8_2:-56577086 Msa1256530:CDS
GCGATAGCATCGATTAATGT+AGG 0.493202 8_2:+56577825 None:intergenic
TTGAGCAAGTGTTACATCGT+TGG 0.495134 8_2:+56578175 None:intergenic
GTTTCTTCAAGTTACACAAA+TGG 0.496382 8_2:-56577536 Msa1256530:CDS
AGTTGAACCTATAGGCAAAT+GGG 0.497812 8_2:-56577078 Msa1256530:CDS
TGATTTAACTTCAAACAACT+TGG 0.502171 8_2:-56577261 Msa1256530:CDS
TCTACTAAAATAACAAGTGT+TGG 0.502927 8_2:+56577293 None:intergenic
ATGCTTGATGAACATGCTGC+TGG 0.503248 8_2:-56576984 Msa1256530:CDS
CTAAGTTGGTGAATTTCGCT+AGG 0.512357 8_2:-56577689 Msa1256530:intron
AATGTGTCTACAGTATCATA+AGG 0.517595 8_2:+56577200 None:intergenic
GGAAGAAGAAGGGTTTGAAA+AGG 0.523598 8_2:+56578331 None:intergenic
ATATGAGTTTGAATTTGATA+GGG 0.529498 8_2:+56577409 None:intergenic
CATATTGTGCTTACTTGGAA+AGG 0.533221 8_2:-56576936 Msa1256530:CDS
TTCTTGTTCATGGAAGAAGA+AGG 0.535894 8_2:+56578320 None:intergenic
TTTCGCTGCACGATTTGTGA+GGG 0.537309 8_2:+56578073 None:intergenic
GCTTTGGATGTTGGGAACCA+TGG 0.537432 8_2:+56578266 None:intergenic
TTGATGAACATGCTGCTGGA+TGG 0.537625 8_2:-56576980 Msa1256530:CDS
TCAAGGGATTGGATTTGGTG+AGG 0.546032 8_2:-56577030 Msa1256530:CDS
TAAGAAAGATCAAGTTTAGG+TGG 0.546147 8_2:+56577726 None:intergenic
TGATCAAAGGAGCAACTTCG+TGG 0.560682 8_2:+56577796 None:intergenic
AGTAGTAGCAGTAGTAGTAG+AGG 0.566106 8_2:+56577370 None:intergenic
TCTTGTTCATGGAAGAAGAA+GGG 0.566584 8_2:+56578321 None:intergenic
TGATTTGAGTCACCATCTTG+TGG 0.581981 8_2:+56578122 None:intergenic
GGATTTGGTGAGGAATGTGT+TGG 0.588738 8_2:-56577020 Msa1256530:CDS
CGGTACCTGTAAAATGCTAG+TGG 0.594873 8_2:-56578048 Msa1256530:CDS
CTTTGGTGACACAAACTGCA+TGG 0.596433 8_2:-56578234 Msa1256530:CDS
GCAATTCTGGAAGCAACAGA+AGG 0.597475 8_2:-56577900 Msa1256530:CDS
TGGTGAGGAATGTGTTGGTG+AGG 0.600886 8_2:-56577015 Msa1256530:CDS
CATGCAGTTTGTGTCACCAA+AGG 0.602334 8_2:+56578235 None:intergenic
ACTTCGTGGATTTAACACCT+TGG 0.617259 8_2:-56577953 Msa1256530:CDS
GAAACAGTGAGTTTGATCAA+AGG 0.618628 8_2:+56577783 None:intergenic
AACAACATAGCACCACAAGA+TGG 0.622233 8_2:-56578134 Msa1256530:CDS
TGCCACGTGGAAGTAAGCAA+AGG 0.638087 8_2:-56577175 Msa1256530:CDS
GCGTACCCGAAACGATGCCA+AGG 0.644547 8_2:+56577936 None:intergenic
CAACGACCTAAGAGACGTAG+TGG 0.660903 8_2:+56577343 None:intergenic
GTGGAAGTAAGCAAAGGCAA+TGG 0.668650 8_2:-56577169 Msa1256530:CDS
TGCCTTTGCTTACTTCCACG+TGG 0.669608 8_2:+56577173 None:intergenic
GGTTTGGTAGGCTTATCTGG+TGG 0.669809 8_2:+56578287 None:intergenic
CTATGTTGTTGAGAACCCAA+AGG 0.670186 8_2:+56578147 None:intergenic
GAGAATGTGATAGCTCATGA+AGG 0.690740 8_2:-56577119 Msa1256530:CDS
CAGATAAGCCTACCAAACCA+TGG 0.694388 8_2:-56578283 Msa1256530:CDS
GTAGACACATTTCTGCCACG+TGG 0.718193 8_2:-56577188 Msa1256530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGAGTTTGAATTTGATA+GGG + chr8_2:56577870-56577889 None:intergenic 20.0%
!! ATATGAGTTTGAATTTGATA+GGG + chr8_2:56577870-56577889 None:intergenic 20.0%
! TCATAAGAAAGATCAAGTTT+AGG + chr8_2:56577556-56577575 None:intergenic 25.0%
! GATCTTTCTTATGAAGAATT+AGG - chr8_2:56577562-56577581 Msa1256530:intron 25.0%
! TTCTTTGATTGAACATTTGA+GGG - chr8_2:56577769-56577788 Msa1256530:CDS 25.0%
! CATATGAGTTTGAATTTGAT+AGG + chr8_2:56577871-56577890 None:intergenic 25.0%
! TCTACTAAAATAACAAGTGT+TGG + chr8_2:56577986-56578005 None:intergenic 25.0%
!! TGATTTAACTTCAAACAACT+TGG - chr8_2:56578015-56578034 Msa1256530:CDS 25.0%
!! ATGAGAAATGCTAATTTTCA+AGG - chr8_2:56578229-56578248 Msa1256530:CDS 25.0%
!! TGAGAAATGCTAATTTTCAA+GGG - chr8_2:56578230-56578249 Msa1256530:CDS 25.0%
! TCATAAGAAAGATCAAGTTT+AGG + chr8_2:56577556-56577575 None:intergenic 25.0%
! GATCTTTCTTATGAAGAATT+AGG - chr8_2:56577562-56577581 Msa1256530:intron 25.0%
! TTCTTTGATTGAACATTTGA+GGG - chr8_2:56577769-56577788 Msa1256530:CDS 25.0%
! CATATGAGTTTGAATTTGAT+AGG + chr8_2:56577871-56577890 None:intergenic 25.0%
! TCTACTAAAATAACAAGTGT+TGG + chr8_2:56577986-56578005 None:intergenic 25.0%
!! TGATTTAACTTCAAACAACT+TGG - chr8_2:56578015-56578034 Msa1256530:CDS 25.0%
!! ATGAGAAATGCTAATTTTCA+AGG - chr8_2:56578229-56578248 Msa1256530:CDS 25.0%
!! TGAGAAATGCTAATTTTCAA+GGG - chr8_2:56578230-56578249 Msa1256530:CDS 25.0%
! TTGAAAAGGTTGTTGTTGTT+TGG + chr8_2:56576934-56576953 None:intergenic 30.0%
TACTTGATTGTTCTTGTTCA+TGG + chr8_2:56576969-56576988 None:intergenic 30.0%
!! TAGTTAAAGCTTTGGATGTT+GGG + chr8_2:56577021-56577040 None:intergenic 30.0%
TAAGAAAGATCAAGTTTAGG+TGG + chr8_2:56577553-56577572 None:intergenic 30.0%
!! TGAAGAATTAGGTTCTAAGT+TGG - chr8_2:56577573-56577592 Msa1256530:intron 30.0%
! GTTTCTTCAAGTTACACAAA+TGG - chr8_2:56577740-56577759 Msa1256530:CDS 30.0%
CTTCTTTGATTGAACATTTG+AGG - chr8_2:56577768-56577787 Msa1256530:CDS 30.0%
GGTCTGCATTTAATTATCTA+TGG - chr8_2:56578050-56578069 Msa1256530:CDS 30.0%
AATGTGTCTACAGTATCATA+AGG + chr8_2:56578079-56578098 None:intergenic 30.0%
!! AATGCTAATTTTCAAGGGAT+TGG - chr8_2:56578235-56578254 Msa1256530:CDS 30.0%
!! AATTTTCAAGGGATTGGATT+TGG - chr8_2:56578241-56578260 Msa1256530:CDS 30.0%
! TTGAAAAGGTTGTTGTTGTT+TGG + chr8_2:56576934-56576953 None:intergenic 30.0%
TACTTGATTGTTCTTGTTCA+TGG + chr8_2:56576969-56576988 None:intergenic 30.0%
!! TAGTTAAAGCTTTGGATGTT+GGG + chr8_2:56577021-56577040 None:intergenic 30.0%
TAAGAAAGATCAAGTTTAGG+TGG + chr8_2:56577553-56577572 None:intergenic 30.0%
!! TGAAGAATTAGGTTCTAAGT+TGG - chr8_2:56577573-56577592 Msa1256530:intron 30.0%
! GTTTCTTCAAGTTACACAAA+TGG - chr8_2:56577740-56577759 Msa1256530:CDS 30.0%
CTTCTTTGATTGAACATTTG+AGG - chr8_2:56577768-56577787 Msa1256530:CDS 30.0%
GGTCTGCATTTAATTATCTA+TGG - chr8_2:56578050-56578069 Msa1256530:CDS 30.0%
AATGTGTCTACAGTATCATA+AGG + chr8_2:56578079-56578098 None:intergenic 30.0%
!! AATGCTAATTTTCAAGGGAT+TGG - chr8_2:56578235-56578254 Msa1256530:CDS 30.0%
!! AATTTTCAAGGGATTGGATT+TGG - chr8_2:56578241-56578260 Msa1256530:CDS 30.0%
!! GTTGTTGTTTGGTGCAAATA+AGG + chr8_2:56576923-56576942 None:intergenic 35.0%
TCTTGTTCATGGAAGAAGAA+GGG + chr8_2:56576958-56576977 None:intergenic 35.0%
TTCTTGTTCATGGAAGAAGA+AGG + chr8_2:56576959-56576978 None:intergenic 35.0%
!! GTAGTTAAAGCTTTGGATGT+TGG + chr8_2:56577022-56577041 None:intergenic 35.0%
! TCCAAAGCTTTAACTACCTT+TGG - chr8_2:56577025-56577044 Msa1256530:CDS 35.0%
ACCAAAGGTAGTTAAAGCTT+TGG + chr8_2:56577029-56577048 None:intergenic 35.0%
ACTTGCTCAACAAATCCTTT+GGG - chr8_2:56577114-56577133 Msa1256530:CDS 35.0%
! TTGTTCCACTAGCATTTTAC+AGG + chr8_2:56577236-56577255 None:intergenic 35.0%
TGAGAATCTGTGTGTATCTA+TGG + chr8_2:56577287-56577306 None:intergenic 35.0%
GAAACAGTGAGTTTGATCAA+AGG + chr8_2:56577496-56577515 None:intergenic 35.0%
GGTTTATTCAAGTGAAGCTT+TGG - chr8_2:56577802-56577821 Msa1256530:CDS 35.0%
GAATTTGATAGGGTTTCATC+TGG + chr8_2:56577860-56577879 None:intergenic 35.0%
ACAACTTGGTCAGTAGATTA+AGG - chr8_2:56578029-56578048 Msa1256530:CDS 35.0%
GGTATGAAGCAGATATTTGT+TGG - chr8_2:56578128-56578147 Msa1256530:CDS 35.0%
AGTTGAACCTATAGGCAAAT+GGG - chr8_2:56578198-56578217 Msa1256530:CDS 35.0%
ATGAGCATATTGTGCTTACT+TGG - chr8_2:56578335-56578354 Msa1256530:CDS 35.0%
CATATTGTGCTTACTTGGAA+AGG - chr8_2:56578340-56578359 Msa1256530:CDS 35.0%
!!! ATGTTGTTTTTGCTTCTGCT+TGG - chr8_2:56578368-56578387 Msa1256530:CDS 35.0%
!! GTTGTTGTTTGGTGCAAATA+AGG + chr8_2:56576923-56576942 None:intergenic 35.0%
TCTTGTTCATGGAAGAAGAA+GGG + chr8_2:56576958-56576977 None:intergenic 35.0%
TTCTTGTTCATGGAAGAAGA+AGG + chr8_2:56576959-56576978 None:intergenic 35.0%
!! GTAGTTAAAGCTTTGGATGT+TGG + chr8_2:56577022-56577041 None:intergenic 35.0%
! TCCAAAGCTTTAACTACCTT+TGG - chr8_2:56577025-56577044 Msa1256530:CDS 35.0%
ACCAAAGGTAGTTAAAGCTT+TGG + chr8_2:56577029-56577048 None:intergenic 35.0%
ACTTGCTCAACAAATCCTTT+GGG - chr8_2:56577114-56577133 Msa1256530:CDS 35.0%
! TTGTTCCACTAGCATTTTAC+AGG + chr8_2:56577236-56577255 None:intergenic 35.0%
TGAGAATCTGTGTGTATCTA+TGG + chr8_2:56577287-56577306 None:intergenic 35.0%
GAAACAGTGAGTTTGATCAA+AGG + chr8_2:56577496-56577515 None:intergenic 35.0%
GGTTTATTCAAGTGAAGCTT+TGG - chr8_2:56577802-56577821 Msa1256530:CDS 35.0%
GAATTTGATAGGGTTTCATC+TGG + chr8_2:56577860-56577879 None:intergenic 35.0%
ACAACTTGGTCAGTAGATTA+AGG - chr8_2:56578029-56578048 Msa1256530:CDS 35.0%
GGTATGAAGCAGATATTTGT+TGG - chr8_2:56578128-56578147 Msa1256530:CDS 35.0%
AGTTGAACCTATAGGCAAAT+GGG - chr8_2:56578198-56578217 Msa1256530:CDS 35.0%
ATGAGCATATTGTGCTTACT+TGG - chr8_2:56578335-56578354 Msa1256530:CDS 35.0%
CATATTGTGCTTACTTGGAA+AGG - chr8_2:56578340-56578359 Msa1256530:CDS 35.0%
!!! ATGTTGTTTTTGCTTCTGCT+TGG - chr8_2:56578368-56578387 Msa1256530:CDS 35.0%
GGAAGAAGAAGGGTTTGAAA+AGG + chr8_2:56576948-56576967 None:intergenic 40.0%
TTGAGCAAGTGTTACATCGT+TGG + chr8_2:56577104-56577123 None:intergenic 40.0%
CACTTGCTCAACAAATCCTT+TGG - chr8_2:56577113-56577132 Msa1256530:CDS 40.0%
CTATGTTGTTGAGAACCCAA+AGG + chr8_2:56577132-56577151 None:intergenic 40.0%
AACAACATAGCACCACAAGA+TGG - chr8_2:56577142-56577161 Msa1256530:CDS 40.0%
TGATTTGAGTCACCATCTTG+TGG + chr8_2:56577157-56577176 None:intergenic 40.0%
! CACTAGCATTTTACAGGTAC+CGG + chr8_2:56577230-56577249 None:intergenic 40.0%
ACTTCGTGGATTTAACACCT+TGG - chr8_2:56577323-56577342 Msa1256530:CDS 40.0%
TTAACACCTTGGCATCGTTT+CGG - chr8_2:56577334-56577353 Msa1256530:CDS 40.0%
!! GCGATAGCATCGATTAATGT+AGG + chr8_2:56577454-56577473 None:intergenic 40.0%
!! CTAAGTTGGTGAATTTCGCT+AGG - chr8_2:56577587-56577606 Msa1256530:intron 40.0%
ACATTTGAGGGTTCAACGTT+TGG - chr8_2:56577781-56577800 Msa1256530:CDS 40.0%
AGTAGTAGCAGTAGTAGTAG+AGG + chr8_2:56577909-56577928 None:intergenic 40.0%
CTATTACCACTACGTCTCTT+AGG - chr8_2:56577927-56577946 Msa1256530:CDS 40.0%
GAGAATGTGATAGCTCATGA+AGG - chr8_2:56578157-56578176 Msa1256530:CDS 40.0%
GTTGAGAGAGTTGAACCTAT+AGG - chr8_2:56578190-56578209 Msa1256530:CDS 40.0%
GAGTTGAACCTATAGGCAAA+TGG - chr8_2:56578197-56578216 Msa1256530:CDS 40.0%
CTTTGTTCCCATTTGCCTAT+AGG + chr8_2:56578208-56578227 None:intergenic 40.0%
GGAAGAAGAAGGGTTTGAAA+AGG + chr8_2:56576948-56576967 None:intergenic 40.0%
TTGAGCAAGTGTTACATCGT+TGG + chr8_2:56577104-56577123 None:intergenic 40.0%
CACTTGCTCAACAAATCCTT+TGG - chr8_2:56577113-56577132 Msa1256530:CDS 40.0%
CTATGTTGTTGAGAACCCAA+AGG + chr8_2:56577132-56577151 None:intergenic 40.0%
AACAACATAGCACCACAAGA+TGG - chr8_2:56577142-56577161 Msa1256530:CDS 40.0%
TGATTTGAGTCACCATCTTG+TGG + chr8_2:56577157-56577176 None:intergenic 40.0%
! CACTAGCATTTTACAGGTAC+CGG + chr8_2:56577230-56577249 None:intergenic 40.0%
ACTTCGTGGATTTAACACCT+TGG - chr8_2:56577323-56577342 Msa1256530:CDS 40.0%
TTAACACCTTGGCATCGTTT+CGG - chr8_2:56577334-56577353 Msa1256530:CDS 40.0%
!! GCGATAGCATCGATTAATGT+AGG + chr8_2:56577454-56577473 None:intergenic 40.0%
!! CTAAGTTGGTGAATTTCGCT+AGG - chr8_2:56577587-56577606 Msa1256530:intron 40.0%
ACATTTGAGGGTTCAACGTT+TGG - chr8_2:56577781-56577800 Msa1256530:CDS 40.0%
AGTAGTAGCAGTAGTAGTAG+AGG + chr8_2:56577909-56577928 None:intergenic 40.0%
CTATTACCACTACGTCTCTT+AGG - chr8_2:56577927-56577946 Msa1256530:CDS 40.0%
GAGAATGTGATAGCTCATGA+AGG - chr8_2:56578157-56578176 Msa1256530:CDS 40.0%
GTTGAGAGAGTTGAACCTAT+AGG - chr8_2:56578190-56578209 Msa1256530:CDS 40.0%
GAGTTGAACCTATAGGCAAA+TGG - chr8_2:56578197-56578216 Msa1256530:CDS 40.0%
CTTTGTTCCCATTTGCCTAT+AGG + chr8_2:56578208-56578227 None:intergenic 40.0%
!! CATGGTTTGGTAGGCTTATC+TGG + chr8_2:56576995-56577014 None:intergenic 45.0%
CAGATAAGCCTACCAAACCA+TGG - chr8_2:56576993-56577012 Msa1256530:CDS 45.0%
CATGCAGTTTGTGTCACCAA+AGG + chr8_2:56577044-56577063 None:intergenic 45.0%
! CTTTGGTGACACAAACTGCA+TGG - chr8_2:56577042-56577061 Msa1256530:CDS 45.0%
TTTCGCTGCACGATTTGTGA+GGG + chr8_2:56577206-56577225 None:intergenic 45.0%
! TTTTCGCTGCACGATTTGTG+AGG + chr8_2:56577207-56577226 None:intergenic 45.0%
ACAAATCGTGCAGCGAAAAC+CGG - chr8_2:56577208-56577227 Msa1256530:CDS 45.0%
CGGTACCTGTAAAATGCTAG+TGG - chr8_2:56577228-56577247 Msa1256530:CDS 45.0%
CATTGAGCTAGCTAACTTCG+TGG - chr8_2:56577309-56577328 Msa1256530:CDS 45.0%
TAACACCTTGGCATCGTTTC+GGG - chr8_2:56577335-56577354 Msa1256530:CDS 45.0%
AGCTGCTAATGCAGCAATTC+TGG - chr8_2:56577363-56577382 Msa1256530:CDS 45.0%
! GCAATTCTGGAAGCAACAGA+AGG - chr8_2:56577376-56577395 Msa1256530:CDS 45.0%
TGATCAAAGGAGCAACTTCG+TGG + chr8_2:56577483-56577502 None:intergenic 45.0%
! TTGTTTCTTAACGCGCTTCG+AGG - chr8_2:56577953-56577972 Msa1256530:CDS 45.0%
GTGGAAGTAAGCAAAGGCAA+TGG - chr8_2:56578107-56578126 Msa1256530:CDS 45.0%
!! TCAAGGGATTGGATTTGGTG+AGG - chr8_2:56578246-56578265 Msa1256530:CDS 45.0%
!! GGATTTGGTGAGGAATGTGT+TGG - chr8_2:56578256-56578275 Msa1256530:CDS 45.0%
ATGCTTGATGAACATGCTGC+TGG - chr8_2:56578292-56578311 Msa1256530:CDS 45.0%
TTGATGAACATGCTGCTGGA+TGG - chr8_2:56578296-56578315 Msa1256530:CDS 45.0%
TGATGAACATGCTGCTGGAT+GGG - chr8_2:56578297-56578316 Msa1256530:CDS 45.0%
!! CATGGTTTGGTAGGCTTATC+TGG + chr8_2:56576995-56577014 None:intergenic 45.0%
CAGATAAGCCTACCAAACCA+TGG - chr8_2:56576993-56577012 Msa1256530:CDS 45.0%
CATGCAGTTTGTGTCACCAA+AGG + chr8_2:56577044-56577063 None:intergenic 45.0%
! CTTTGGTGACACAAACTGCA+TGG - chr8_2:56577042-56577061 Msa1256530:CDS 45.0%
TTTCGCTGCACGATTTGTGA+GGG + chr8_2:56577206-56577225 None:intergenic 45.0%
! TTTTCGCTGCACGATTTGTG+AGG + chr8_2:56577207-56577226 None:intergenic 45.0%
ACAAATCGTGCAGCGAAAAC+CGG - chr8_2:56577208-56577227 Msa1256530:CDS 45.0%
CGGTACCTGTAAAATGCTAG+TGG - chr8_2:56577228-56577247 Msa1256530:CDS 45.0%
CATTGAGCTAGCTAACTTCG+TGG - chr8_2:56577309-56577328 Msa1256530:CDS 45.0%
TAACACCTTGGCATCGTTTC+GGG - chr8_2:56577335-56577354 Msa1256530:CDS 45.0%
AGCTGCTAATGCAGCAATTC+TGG - chr8_2:56577363-56577382 Msa1256530:CDS 45.0%
! GCAATTCTGGAAGCAACAGA+AGG - chr8_2:56577376-56577395 Msa1256530:CDS 45.0%
TGATCAAAGGAGCAACTTCG+TGG + chr8_2:56577483-56577502 None:intergenic 45.0%
! TTGTTTCTTAACGCGCTTCG+AGG - chr8_2:56577953-56577972 Msa1256530:CDS 45.0%
GTGGAAGTAAGCAAAGGCAA+TGG - chr8_2:56578107-56578126 Msa1256530:CDS 45.0%
!! TCAAGGGATTGGATTTGGTG+AGG - chr8_2:56578246-56578265 Msa1256530:CDS 45.0%
!! GGATTTGGTGAGGAATGTGT+TGG - chr8_2:56578256-56578275 Msa1256530:CDS 45.0%
ATGCTTGATGAACATGCTGC+TGG - chr8_2:56578292-56578311 Msa1256530:CDS 45.0%
TTGATGAACATGCTGCTGGA+TGG - chr8_2:56578296-56578315 Msa1256530:CDS 45.0%
TGATGAACATGCTGCTGGAT+GGG - chr8_2:56578297-56578316 Msa1256530:CDS 45.0%
! GGTTTGGTAGGCTTATCTGG+TGG + chr8_2:56576992-56577011 None:intergenic 50.0%
!! GTTGGGAACCATGGTTTGGT+AGG + chr8_2:56577004-56577023 None:intergenic 50.0%
GGATGTTGGGAACCATGGTT+TGG + chr8_2:56577008-56577027 None:intergenic 50.0%
! GCTTTGGATGTTGGGAACCA+TGG + chr8_2:56577013-56577032 None:intergenic 50.0%
CACGATTTGTGAGGGCTCTA+AGG + chr8_2:56577198-56577217 None:intergenic 50.0%
CAACGACCTAAGAGACGTAG+TGG + chr8_2:56577936-56577955 None:intergenic 50.0%
GTAGACACATTTCTGCCACG+TGG - chr8_2:56578088-56578107 Msa1256530:CDS 50.0%
TGCCACGTGGAAGTAAGCAA+AGG - chr8_2:56578101-56578120 Msa1256530:CDS 50.0%
TGCCTTTGCTTACTTCCACG+TGG + chr8_2:56578106-56578125 None:intergenic 50.0%
! TGGTGAGGAATGTGTTGGTG+AGG - chr8_2:56578261-56578280 Msa1256530:CDS 50.0%
GATGAACATGCTGCTGGATG+GGG - chr8_2:56578298-56578317 Msa1256530:CDS 50.0%
! GGTTTGGTAGGCTTATCTGG+TGG + chr8_2:56576992-56577011 None:intergenic 50.0%
!! GTTGGGAACCATGGTTTGGT+AGG + chr8_2:56577004-56577023 None:intergenic 50.0%
GGATGTTGGGAACCATGGTT+TGG + chr8_2:56577008-56577027 None:intergenic 50.0%
! GCTTTGGATGTTGGGAACCA+TGG + chr8_2:56577013-56577032 None:intergenic 50.0%
CACGATTTGTGAGGGCTCTA+AGG + chr8_2:56577198-56577217 None:intergenic 50.0%
CAACGACCTAAGAGACGTAG+TGG + chr8_2:56577936-56577955 None:intergenic 50.0%
GTAGACACATTTCTGCCACG+TGG - chr8_2:56578088-56578107 Msa1256530:CDS 50.0%
TGCCACGTGGAAGTAAGCAA+AGG - chr8_2:56578101-56578120 Msa1256530:CDS 50.0%
TGCCTTTGCTTACTTCCACG+TGG + chr8_2:56578106-56578125 None:intergenic 50.0%
! TGGTGAGGAATGTGTTGGTG+AGG - chr8_2:56578261-56578280 Msa1256530:CDS 50.0%
GATGAACATGCTGCTGGATG+GGG - chr8_2:56578298-56578317 Msa1256530:CDS 50.0%
GCGTACCCGAAACGATGCCA+AGG + chr8_2:56577343-56577362 None:intergenic 60.0%
GCGTACCCGAAACGATGCCA+AGG + chr8_2:56577343-56577362 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 56576896 56578402 56576896 ID=Msa1256530;Name=Msa1256530
chr8_2 mRNA 56576896 56578402 56576896 ID=Msa1256530-mRNA-1;Parent=Msa1256530;Name=Msa1256530-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|459
chr8_2 exon 56577690 56578402 56577690 ID=Msa1256530-mRNA-1:exon:17442;Parent=Msa1256530-mRNA-1
chr8_2 exon 56576896 56577562 56576896 ID=Msa1256530-mRNA-1:exon:17441;Parent=Msa1256530-mRNA-1
chr8_2 CDS 56577690 56578402 56577690 ID=Msa1256530-mRNA-1:cds;Parent=Msa1256530-mRNA-1
chr8_2 CDS 56576896 56577562 56576896 ID=Msa1256530-mRNA-1:cds;Parent=Msa1256530-mRNA-1
Gene Sequence

>Msa1256530

ATGACGCAATTCACACATTCACTACCTTATTTGCACCAAACAACAACAACCTTTTCAAACCCTTCTTCTTCCATGAACAAGAACAATCAAGTACCACCAGATAAGCCTACCAAACCATGGTTCCCAACATCCAAAGCTTTAACTACCTTTGGTGACACAAACTGCATGGAACAGTTACTTGTTCATTGCGCAAACGCAATCGAAACCAACGATGTAACACTTGCTCAACAAATCCTTTGGGTTCTCAACAACATAGCACCACAAGATGGTGACTCAAATCAACGCTTAGCTTATAGTTTCCTTAGAGCCCTCACAAATCGTGCAGCGAAAACCGGTACCTGTAAAATGCTAGTGGAACAAGTTTATTCAAATGCTCATAACAATCTCACCATAGATACACACAGATTCTCAGTCATTGAGCTAGCTAACTTCGTGGATTTAACACCTTGGCATCGTTTCGGGTACGCAGCTGCTAATGCAGCAATTCTGGAAGCAACAGAAGGTTTCTCAGTGATTCATATAGTTGATTTGAGTTCAACTCATTGCATGCAGATTCCTACATTAATCGATGCTATCGCTAATCGCCACGAAGTTGCTCCTTTGATCAAACTCACTGTTTCTGATCATGCTAACAACAACAGTGATCAACTAATTCCACCTAAACTTGATCTTTCTTATGAAGAATTAGGTTCTAAGTTGGTGAATTTCGCTAGAGTAGTTTCTTCAAGTTACACAAATGGTTTTGCTTCTTTGATTGAACATTTGAGGGTTCAACGTTTGGTTTATTCAAGTGAAGCTTTGGTTATAAATTGTCACATGATGCTTCATTATATTCCAGATGAAACCCTATCAAATTCAAACTCATATGTTTATGATTCTATGTCCTCTACTACTACTGCTACTACTATTACCACTACGTCTCTTAGGTCGTTGTTTCTTAACGCGCTTCGAGGTTTAGATCCAACACTTGTTATTTTAGTAGATGAAGATGTTGATTTAACTTCAAACAACTTGGTCAGTAGATTAAGGTCTGCATTTAATTATCTATGGATACCTTATGATACTGTAGACACATTTCTGCCACGTGGAAGTAAGCAAAGGCAATGGTATGAAGCAGATATTTGTTGGAAGATTGAGAATGTGATAGCTCATGAAGGTGTTCAAAGAGTTGAGAGAGTTGAACCTATAGGCAAATGGGAACAAAGAATGAGAAATGCTAATTTTCAAGGGATTGGATTTGGTGAGGAATGTGTTGGTGAGGTGAAAGCTATGCTTGATGAACATGCTGCTGGATGGGGATTGAAGAAAGAAGATGAGCATATTGTGCTTACTTGGAAAGGACATAATGTTGTTTTTGCTTCTGCTTGGTTACCTGTTTAA
Protein sequence

>Msa1256530

MTQFTHSLPYLHQTTTTFSNPSSSMNKNNQVPPDKPTKPWFPTSKALTTFGDTNCMEQLLVHCANAIETNDVTLAQQILWVLNNIAPQDGDSNQRLAYSFLRALTNRAAKTGTCKMLVEQVYSNAHNNLTIDTHRFSVIELANFVDLTPWHRFGYAAANAAILEATEGFSVIHIVDLSSTHCMQIPTLIDAIANRHEVAPLIKLTVSDHANNNSDQLIPPKLDLSYEELGSKLVNFARVVSSSYTNGFASLIEHLRVQRLVYSSEALVINCHMMLHYIPDETLSNSNSYVYDSMSSTTTATTITTTSLRSLFLNALRGLDPTLVILVDEDVDLTSNNLVSRLRSAFNYLWIPYDTVDTFLPRGSKQRQWYEADICWKIENVIAHEGVQRVERVEPIGKWEQRMRNANFQGIGFGEECVGEVKAMLDEHAAGWGLKKEDEHIVLTWKGHNVVFASAWLPV*