Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1259630 | XP_013457865.1 | 98.662 | 299 | 4 | 0 | 1 | 299 | 1 | 299 | 0.0 | 617 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1259630 | sp|Q9STS6|LBD27_ARATH | 39.216 | 306 | 138 | 8 | 1 | 262 | 27 | 328 | 1.54e-55 | 185 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1259630 | A0A072URD2 | 98.662 | 299 | 4 | 0 | 1 | 299 | 1 | 299 | 0.0 | 617 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1259630 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1259630 | MtrunA17_Chr4g0059901 | 98.491 | 265 | 4 | 0 | 1 | 265 | 1 | 265 | 0.0 | 550 |
| Msa1259630 | MtrunA17_Chr8g0342351 | 58.333 | 108 | 45 | 0 | 7 | 114 | 9 | 116 | 8.62e-36 | 129 |
| Msa1259630 | MtrunA17_Chr6g0452671 | 51.786 | 112 | 54 | 0 | 4 | 115 | 5 | 116 | 2.55e-35 | 126 |
| Msa1259630 | MtrunA17_Chr6g0452661 | 50.000 | 114 | 56 | 1 | 9 | 121 | 8 | 121 | 9.44e-33 | 121 |
| Msa1259630 | MtrunA17_Chr6g0452691 | 49.138 | 116 | 58 | 1 | 7 | 121 | 6 | 121 | 8.10e-32 | 117 |
| Msa1259630 | MtrunA17_Chr5g0439111 | 51.304 | 115 | 55 | 1 | 9 | 122 | 8 | 122 | 9.51e-31 | 115 |
| Msa1259630 | MtrunA17_Chr7g0227511 | 37.580 | 157 | 86 | 4 | 11 | 162 | 12 | 161 | 2.21e-30 | 112 |
| Msa1259630 | MtrunA17_Chr2g0330201 | 50.459 | 109 | 54 | 0 | 6 | 114 | 15 | 123 | 3.97e-30 | 115 |
| Msa1259630 | MtrunA17_Chr4g0055441 | 47.664 | 107 | 55 | 1 | 11 | 116 | 3 | 109 | 1.06e-28 | 107 |
| Msa1259630 | MtrunA17_Chr3g0117121 | 48.000 | 100 | 52 | 0 | 11 | 110 | 11 | 110 | 1.23e-28 | 108 |
| Msa1259630 | MtrunA17_Chr4g0058651 | 48.958 | 96 | 49 | 0 | 11 | 106 | 12 | 107 | 1.62e-28 | 108 |
| Msa1259630 | MtrunA17_Chr4g0043421 | 48.000 | 100 | 52 | 0 | 4 | 103 | 9 | 108 | 2.75e-28 | 107 |
| Msa1259630 | MtrunA17_Chr5g0437571 | 43.220 | 118 | 66 | 1 | 6 | 123 | 2 | 118 | 9.16e-28 | 105 |
| Msa1259630 | MtrunA17_Chr6g0449651 | 44.545 | 110 | 61 | 0 | 1 | 110 | 58 | 167 | 1.02e-27 | 106 |
| Msa1259630 | MtrunA17_Chr6g0449641 | 47.000 | 100 | 53 | 0 | 11 | 110 | 10 | 109 | 1.92e-27 | 104 |
| Msa1259630 | MtrunA17_Chr3g0106101 | 43.478 | 115 | 64 | 1 | 5 | 119 | 2 | 115 | 3.79e-27 | 104 |
| Msa1259630 | MtrunA17_Chr8g0353981 | 38.037 | 163 | 98 | 1 | 11 | 173 | 8 | 167 | 4.55e-27 | 105 |
| Msa1259630 | MtrunA17_Chr2g0311601 | 46.000 | 100 | 54 | 0 | 4 | 103 | 7 | 106 | 7.65e-27 | 103 |
| Msa1259630 | MtrunA17_Chr3g0114061 | 42.857 | 119 | 67 | 1 | 3 | 121 | 1 | 118 | 1.24e-26 | 102 |
| Msa1259630 | MtrunA17_Chr5g0402611 | 43.363 | 113 | 64 | 0 | 11 | 123 | 9 | 121 | 1.12e-25 | 103 |
| Msa1259630 | MtrunA17_Chr3g0115431 | 45.000 | 100 | 55 | 0 | 11 | 110 | 10 | 109 | 1.68e-24 | 98.6 |
| Msa1259630 | MtrunA17_Chr4g0040831 | 39.474 | 114 | 62 | 1 | 6 | 119 | 11 | 117 | 2.96e-24 | 97.4 |
| Msa1259630 | MtrunA17_Chr5g0438091 | 37.705 | 122 | 76 | 0 | 7 | 128 | 36 | 157 | 9.49e-24 | 95.9 |
| Msa1259630 | MtrunA17_Chr7g0260971 | 41.509 | 106 | 61 | 1 | 6 | 110 | 10 | 115 | 4.22e-23 | 94.7 |
| Msa1259630 | MtrunA17_Chr1g0200201 | 43.000 | 100 | 57 | 0 | 11 | 110 | 73 | 172 | 6.90e-23 | 94.4 |
| Msa1259630 | MtrunA17_Chr3g0097221 | 45.000 | 100 | 55 | 0 | 11 | 110 | 8 | 107 | 1.07e-22 | 95.5 |
| Msa1259630 | MtrunA17_Chr4g0030001 | 45.833 | 96 | 47 | 2 | 11 | 105 | 25 | 116 | 1.94e-22 | 92.4 |
| Msa1259630 | MtrunA17_Chr5g0437401 | 38.318 | 107 | 66 | 0 | 7 | 113 | 8 | 114 | 5.10e-22 | 90.9 |
| Msa1259630 | MtrunA17_Chr5g0444351 | 40.909 | 110 | 64 | 1 | 11 | 120 | 6 | 114 | 7.24e-22 | 89.4 |
| Msa1259630 | MtrunA17_Chr7g0225111 | 41.000 | 100 | 59 | 0 | 11 | 110 | 6 | 105 | 3.84e-21 | 86.7 |
| Msa1259630 | MtrunA17_Chr3g0113891 | 40.206 | 97 | 58 | 0 | 7 | 103 | 11 | 107 | 5.11e-21 | 88.2 |
| Msa1259630 | MtrunA17_Chr3g0129071 | 38.462 | 104 | 64 | 0 | 7 | 110 | 44 | 147 | 1.06e-20 | 88.6 |
| Msa1259630 | MtrunA17_Chr5g0432451 | 42.308 | 104 | 60 | 0 | 7 | 110 | 40 | 143 | 5.48e-20 | 86.7 |
| Msa1259630 | MtrunA17_Chr8g0374591 | 38.462 | 104 | 64 | 0 | 7 | 110 | 5 | 108 | 6.61e-20 | 83.6 |
| Msa1259630 | MtrunA17_Chr6g0460951 | 41.584 | 101 | 58 | 1 | 11 | 110 | 41 | 141 | 2.21e-19 | 85.1 |
| Msa1259630 | MtrunA17_Chr8g0351721 | 39.640 | 111 | 62 | 2 | 5 | 110 | 9 | 119 | 3.60e-19 | 84.0 |
| Msa1259630 | MtrunA17_Chr5g0435641 | 38.384 | 99 | 60 | 1 | 6 | 103 | 3 | 101 | 6.40e-19 | 82.8 |
| Msa1259630 | MtrunA17_Chr8g0351701 | 41.489 | 94 | 54 | 1 | 11 | 103 | 24 | 117 | 1.50e-18 | 82.8 |
| Msa1259630 | MtrunA17_Chr1g0184281 | 41.000 | 100 | 58 | 1 | 11 | 109 | 16 | 115 | 2.96e-18 | 81.3 |
| Msa1259630 | MtrunA17_Chr8g0374601 | 38.235 | 102 | 63 | 0 | 9 | 110 | 25 | 126 | 5.32e-18 | 82.0 |
| Msa1259630 | MtrunA17_Chr1g0184271 | 39.000 | 100 | 60 | 1 | 11 | 109 | 13 | 112 | 1.17e-17 | 80.5 |
| Msa1259630 | MtrunA17_Chr7g0261031 | 37.143 | 105 | 65 | 1 | 6 | 109 | 4 | 108 | 2.85e-17 | 79.0 |
| Msa1259630 | MtrunA17_Chr7g0245871 | 39.362 | 94 | 56 | 1 | 11 | 103 | 28 | 121 | 3.14e-17 | 79.3 |
| Msa1259630 | MtrunA17_Chr6g0460941 | 41.379 | 87 | 50 | 1 | 11 | 96 | 8 | 94 | 2.47e-16 | 73.2 |
| Msa1259630 | MtrunA17_Chr8g0374581 | 38.835 | 103 | 62 | 1 | 9 | 110 | 7 | 109 | 6.42e-16 | 75.5 |
| Msa1259630 | MtrunA17_Chr2g0326041 | 52.083 | 48 | 23 | 0 | 11 | 58 | 34 | 81 | 1.36e-13 | 65.5 |
| Msa1259630 | MtrunA17_Chr6g0449311 | 33.083 | 133 | 86 | 2 | 38 | 167 | 1 | 133 | 3.74e-12 | 63.2 |
| Msa1259630 | MtrunA17_Chr4g0046201 | 33.333 | 87 | 58 | 0 | 10 | 96 | 6 | 92 | 4.96e-12 | 65.1 |
| Msa1259630 | MtrunA17_Chr7g0245861 | 38.202 | 89 | 52 | 2 | 11 | 96 | 13 | 101 | 3.03e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1259630 | AT3G47870.1 | 39.216 | 306 | 138 | 8 | 1 | 262 | 27 | 328 | 1.56e-56 | 185 |
| Msa1259630 | AT3G13850.1 | 46.452 | 155 | 68 | 2 | 9 | 161 | 35 | 176 | 4.99e-36 | 130 |
| Msa1259630 | AT1G72980.1 | 47.287 | 129 | 63 | 1 | 2 | 125 | 5 | 133 | 1.26e-33 | 122 |
| Msa1259630 | AT5G63090.4 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.04e-28 | 108 |
| Msa1259630 | AT5G63090.3 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.04e-28 | 108 |
| Msa1259630 | AT5G63090.2 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.04e-28 | 108 |
| Msa1259630 | AT5G63090.1 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.04e-28 | 108 |
| Msa1259630 | AT3G27650.1 | 47.959 | 98 | 51 | 0 | 7 | 104 | 36 | 133 | 6.69e-28 | 106 |
| Msa1259630 | AT1G65620.1 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.06e-27 | 107 |
| Msa1259630 | AT1G65620.4 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.06e-27 | 107 |
| Msa1259630 | AT1G65620.3 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.06e-27 | 107 |
| Msa1259630 | AT1G65620.5 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.06e-27 | 107 |
| Msa1259630 | AT1G65620.2 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.06e-27 | 107 |
| Msa1259630 | AT2G30130.1 | 41.667 | 120 | 69 | 1 | 3 | 122 | 1 | 119 | 1.96e-26 | 103 |
| Msa1259630 | AT5G66870.1 | 44.231 | 104 | 58 | 0 | 7 | 110 | 4 | 107 | 1.12e-25 | 104 |
| Msa1259630 | AT1G31320.1 | 47.312 | 93 | 49 | 0 | 4 | 96 | 7 | 99 | 2.25e-25 | 100 |
| Msa1259630 | AT2G28500.1 | 42.105 | 114 | 60 | 2 | 11 | 122 | 56 | 165 | 9.33e-23 | 94.7 |
| Msa1259630 | AT2G30340.2 | 40.909 | 110 | 63 | 1 | 7 | 116 | 50 | 157 | 9.40e-23 | 95.5 |
| Msa1259630 | AT2G30340.1 | 40.909 | 110 | 63 | 1 | 7 | 116 | 49 | 156 | 1.09e-22 | 95.1 |
| Msa1259630 | AT1G07900.1 | 46.316 | 95 | 47 | 1 | 11 | 105 | 34 | 124 | 1.75e-22 | 92.8 |
| Msa1259630 | AT1G16530.1 | 41.284 | 109 | 61 | 2 | 5 | 112 | 9 | 115 | 2.90e-22 | 91.7 |
| Msa1259630 | AT2G23660.2 | 42.000 | 100 | 58 | 0 | 11 | 110 | 6 | 105 | 8.20e-22 | 93.6 |
| Msa1259630 | AT2G23660.3 | 42.000 | 100 | 58 | 0 | 11 | 110 | 6 | 105 | 8.20e-22 | 93.6 |
| Msa1259630 | AT2G23660.1 | 42.000 | 100 | 58 | 0 | 11 | 110 | 6 | 105 | 8.20e-22 | 93.6 |
| Msa1259630 | AT3G11090.1 | 35.000 | 120 | 78 | 0 | 7 | 126 | 8 | 127 | 4.17e-21 | 88.2 |
| Msa1259630 | AT3G03760.1 | 41.584 | 101 | 58 | 1 | 11 | 110 | 52 | 152 | 6.74e-21 | 90.5 |
| Msa1259630 | AT3G50510.1 | 42.000 | 100 | 58 | 0 | 11 | 110 | 13 | 112 | 1.47e-20 | 87.8 |
| Msa1259630 | AT3G50510.2 | 42.000 | 100 | 58 | 0 | 11 | 110 | 13 | 112 | 1.47e-20 | 87.8 |
| Msa1259630 | AT1G06280.1 | 47.619 | 84 | 44 | 0 | 9 | 92 | 23 | 106 | 2.08e-20 | 87.4 |
| Msa1259630 | AT2G40470.2 | 39.655 | 116 | 70 | 0 | 7 | 122 | 17 | 132 | 3.86e-20 | 86.7 |
| Msa1259630 | AT2G40470.1 | 40.541 | 111 | 66 | 0 | 7 | 117 | 42 | 152 | 1.02e-19 | 86.3 |
| Msa1259630 | AT5G06080.1 | 33.333 | 165 | 103 | 4 | 10 | 170 | 7 | 168 | 2.21e-19 | 84.0 |
| Msa1259630 | AT4G00210.1 | 39.623 | 106 | 63 | 1 | 6 | 110 | 7 | 112 | 3.81e-19 | 84.3 |
| Msa1259630 | AT5G15060.1 | 45.570 | 79 | 41 | 1 | 5 | 81 | 12 | 90 | 6.84e-19 | 81.6 |
| Msa1259630 | AT3G26660.1 | 34.234 | 111 | 73 | 0 | 11 | 121 | 6 | 116 | 1.83e-18 | 80.1 |
| Msa1259630 | AT2G42430.1 | 36.792 | 106 | 66 | 1 | 6 | 110 | 11 | 116 | 1.84e-18 | 83.2 |
| Msa1259630 | AT2G45420.1 | 40.385 | 104 | 58 | 2 | 13 | 112 | 40 | 143 | 2.60e-18 | 83.2 |
| Msa1259630 | AT2G42440.1 | 35.593 | 118 | 68 | 2 | 6 | 122 | 3 | 113 | 2.87e-18 | 82.8 |
| Msa1259630 | AT4G00220.1 | 40.385 | 104 | 58 | 2 | 13 | 112 | 20 | 123 | 4.39e-18 | 81.6 |
| Msa1259630 | AT3G26620.1 | 33.036 | 112 | 75 | 0 | 11 | 122 | 6 | 117 | 4.51e-18 | 79.0 |
| Msa1259630 | AT3G58190.1 | 41.000 | 100 | 58 | 1 | 11 | 109 | 12 | 111 | 4.89e-18 | 81.3 |
| Msa1259630 | AT5G35900.1 | 31.000 | 100 | 69 | 0 | 11 | 110 | 6 | 105 | 1.21e-16 | 77.4 |
| Msa1259630 | AT2G31310.1 | 29.412 | 153 | 94 | 5 | 5 | 144 | 2 | 153 | 1.93e-16 | 76.3 |
| Msa1259630 | AT2G19820.1 | 33.673 | 98 | 59 | 2 | 11 | 107 | 13 | 105 | 6.01e-15 | 70.5 |
| Msa1259630 | AT2G45410.1 | 39.394 | 99 | 59 | 1 | 13 | 110 | 19 | 117 | 6.94e-15 | 72.0 |
Find 55 sgRNAs with CRISPR-Local
Find 158 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGTTCACAAATTATT+TGG | 0.102603 | 8_2:-61355433 | Msa1259630:CDS |
| CAAATAATTTGTGAACATTT+TGG | 0.141519 | 8_2:+61355434 | None:intergenic |
| CAGGTGTGCACTTTCTTCTT+TGG | 0.204290 | 8_2:+61355503 | None:intergenic |
| TTATCATTCAAGCTAATTAT+AGG | 0.238366 | 8_2:-61355342 | Msa1259630:CDS |
| GTGACTTCACAGTTAGAATT+AGG | 0.249617 | 8_2:-61355172 | Msa1259630:CDS |
| ATCAACAACTTGAAATTTGC+AGG | 0.281961 | 8_2:-61355231 | Msa1259630:CDS |
| GGTTGATCTGCAGGGAAATA+AGG | 0.290053 | 8_2:+61355466 | None:intergenic |
| GCAGGCTACAATATCAAATA+TGG | 0.297786 | 8_2:-61355282 | Msa1259630:CDS |
| AGCAATGCAACCTCATAAAT+TGG | 0.298388 | 8_2:-61354912 | Msa1259630:CDS |
| GCAATCATACATTTATTCTA+AGG | 0.300515 | 8_2:-61354792 | Msa1259630:CDS |
| TATCATTCAAGCTAATTATA+GGG | 0.334002 | 8_2:-61355341 | Msa1259630:CDS |
| AATAATAATAATGACCCTTA+AGG | 0.352659 | 8_2:-61355560 | None:intergenic |
| CTTGTGTTGTGCCACCCTTA+AGG | 0.355961 | 8_2:+61355545 | None:intergenic |
| GGGCACTGAAGCCACAAATT+AGG | 0.358880 | 8_2:+61354962 | None:intergenic |
| TTGTGTTGTGCCACCCTTAA+GGG | 0.363348 | 8_2:+61355546 | None:intergenic |
| TTGGAATTTGCATGCTGCAC+AGG | 0.387931 | 8_2:+61355522 | None:intergenic |
| TATCTTCAACAACTCCTTGT+TGG | 0.395214 | 8_2:+61354858 | None:intergenic |
| CTGCTATTGTTATTTGTGTA+AGG | 0.395645 | 8_2:+61354941 | None:intergenic |
| CTTCATGAGGTAACTGTTGT+TGG | 0.400649 | 8_2:+61355197 | None:intergenic |
| ATAATAATAATGACCCTTAA+GGG | 0.421007 | 8_2:-61355559 | None:intergenic |
| TTGTTATAAGTCTGTGGTTG+TGG | 0.430309 | 8_2:+61355103 | None:intergenic |
| ATGCTACAAATCCTAATTTG+TGG | 0.446980 | 8_2:-61354973 | Msa1259630:CDS |
| AAAGAGTGCGAGCGCATTGA+AGG | 0.447294 | 8_2:+61355135 | None:intergenic |
| GATGTTGTGAGGAAATTTGC+AGG | 0.467829 | 8_2:-61355300 | Msa1259630:CDS |
| TCTGAGGTAACCAATTTATG+AGG | 0.490694 | 8_2:+61354902 | None:intergenic |
| ACAATATCAAATATGGATGA+TGG | 0.492612 | 8_2:-61355275 | Msa1259630:CDS |
| CAATAACTCCTCCCTCTACA+TGG | 0.493365 | 8_2:-61355008 | Msa1259630:CDS |
| CAGGGAAATAAGGAGCTAGA+AGG | 0.497052 | 8_2:+61355476 | None:intergenic |
| TGCTATTGTTATTTGTGTAA+GGG | 0.508900 | 8_2:+61354942 | None:intergenic |
| TTGAATGATAATAGAATCCA+TGG | 0.528060 | 8_2:+61355354 | None:intergenic |
| CTCCCTCTACATGGATTCAA+AGG | 0.529912 | 8_2:-61354999 | Msa1259630:CDS |
| GTAATCCACACTATTGCTGT+TGG | 0.537932 | 8_2:+61355030 | None:intergenic |
| ATCATACATTTATTCTAAGG+AGG | 0.554320 | 8_2:-61354789 | Msa1259630:CDS |
| CTGATCAGTTGACACTGGTA+AGG | 0.557706 | 8_2:+61354383 | None:intergenic |
| TTATTGTTGTTATAAGTCTG+TGG | 0.569885 | 8_2:+61355097 | None:intergenic |
| TGTCAAAATATTAAAGTACC+TGG | 0.571068 | 8_2:-61355398 | Msa1259630:CDS |
| TCCTCACAACATCCATGCAC+AGG | 0.573785 | 8_2:+61355310 | None:intergenic |
| CACACTATTGCTGTTGGAGT+AGG | 0.575688 | 8_2:+61355036 | None:intergenic |
| CCTTGTTGGACAGATGATAG+TGG | 0.578651 | 8_2:+61354872 | None:intergenic |
| AGGGATAAGTATCCTGTGCA+TGG | 0.583412 | 8_2:-61355322 | Msa1259630:CDS |
| TTCACAGTTAGAATTAGGAA+TGG | 0.586596 | 8_2:-61355167 | Msa1259630:CDS |
| ATCCTTTGAATCCATGTAGA+GGG | 0.590065 | 8_2:+61354997 | None:intergenic |
| TAGTCAGAAGAAAACAGCCA+TGG | 0.610751 | 8_2:-61355371 | Msa1259630:CDS |
| CTACTCCAACAGCAATAGTG+TGG | 0.620940 | 8_2:-61355035 | Msa1259630:CDS |
| TAAGGAGCTAGAAGGCACTC+AGG | 0.632470 | 8_2:+61355484 | None:intergenic |
| CATCCTTTGAATCCATGTAG+AGG | 0.632560 | 8_2:+61354996 | None:intergenic |
| CCACTATCATCTGTCCAACA+AGG | 0.637452 | 8_2:-61354872 | Msa1259630:CDS |
| TGATAGTGGCTGCGACTCTG+AGG | 0.643276 | 8_2:+61354886 | None:intergenic |
| CTAAGGAGGCTTATGAATCG+AGG | 0.657710 | 8_2:-61354775 | Msa1259630:intron |
| TAGTACTGATCAGTTGACAC+TGG | 0.662425 | 8_2:+61354378 | None:intergenic |
| CTTTGAATCCATGTAGAGGG+AGG | 0.666923 | 8_2:+61355000 | None:intergenic |
| TGTGAAGTCACATCTTCATG+AGG | 0.677402 | 8_2:+61355184 | None:intergenic |
| ATAATAATGACCCTTAAGGG+TGG | 0.680795 | 8_2:-61355556 | None:intergenic |
| TCCTGTGCATGGATGTTGTG+AGG | 0.692042 | 8_2:-61355311 | Msa1259630:CDS |
| TGTTGCTGAGATACAGACAA+AGG | 0.703585 | 8_2:+61355061 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAACATTTTGGAAAATTTT+GGG | + | chr8_2:61354495-61354514 | None:intergenic | 20.0% |
| !!! | CAAATAATTTGTGAACATTT+TGG | + | chr8_2:61354506-61354525 | None:intergenic | 20.0% |
| !! | CAAAATGTTCACAAATTATT+TGG | - | chr8_2:61354504-61354523 | Msa1259630:intron | 20.0% |
| !! | TTATCATTCAAGCTAATTAT+AGG | - | chr8_2:61354595-61354614 | Msa1259630:intron | 20.0% |
| !! | TATCATTCAAGCTAATTATA+GGG | - | chr8_2:61354596-61354615 | Msa1259630:intron | 20.0% |
| !! | AATGTTGAAATTAGATATAG+TGG | - | chr8_2:61355361-61355380 | Msa1259630:CDS | 20.0% |
| !! | ATGTTGAAATTAGATATAGT+GGG | - | chr8_2:61355362-61355381 | Msa1259630:CDS | 20.0% |
| !!! | TGAACATTTTGGAAAATTTT+GGG | + | chr8_2:61354495-61354514 | None:intergenic | 20.0% |
| !!! | CAAATAATTTGTGAACATTT+TGG | + | chr8_2:61354506-61354525 | None:intergenic | 20.0% |
| !! | CAAAATGTTCACAAATTATT+TGG | - | chr8_2:61354504-61354523 | Msa1259630:intron | 20.0% |
| !! | TTATCATTCAAGCTAATTAT+AGG | - | chr8_2:61354595-61354614 | Msa1259630:intron | 20.0% |
| !! | TATCATTCAAGCTAATTATA+GGG | - | chr8_2:61354596-61354615 | Msa1259630:intron | 20.0% |
| !! | AATGTTGAAATTAGATATAG+TGG | - | chr8_2:61355361-61355380 | Msa1259630:CDS | 20.0% |
| !! | ATGTTGAAATTAGATATAGT+GGG | - | chr8_2:61355362-61355381 | Msa1259630:CDS | 20.0% |
| !!! | GTGAACATTTTGGAAAATTT+TGG | + | chr8_2:61354496-61354515 | None:intergenic | 25.0% |
| !! | TGTCAAAATATTAAAGTACC+TGG | - | chr8_2:61354539-61354558 | Msa1259630:intron | 25.0% |
| ! | TTGAATGATAATAGAATCCA+TGG | + | chr8_2:61354586-61354605 | None:intergenic | 25.0% |
| ! | ACAATATCAAATATGGATGA+TGG | - | chr8_2:61354662-61354681 | Msa1259630:intron | 25.0% |
| !! | TTATTGTTGTTATAAGTCTG+TGG | + | chr8_2:61354843-61354862 | None:intergenic | 25.0% |
| !!! | TGCTATTGTTATTTGTGTAA+GGG | + | chr8_2:61354998-61355017 | None:intergenic | 25.0% |
| !!! | TTTTTGATACAGTTGATGAT+AGG | - | chr8_2:61355123-61355142 | Msa1259630:CDS | 25.0% |
| ! | GCAATCATACATTTATTCTA+AGG | - | chr8_2:61355145-61355164 | Msa1259630:CDS | 25.0% |
| !! | ATCATACATTTATTCTAAGG+AGG | - | chr8_2:61355148-61355167 | Msa1259630:CDS | 25.0% |
| !!! | GTGTTTTTGATCTACTTTAA+CGG | + | chr8_2:61355226-61355245 | None:intergenic | 25.0% |
| !!! | GTGAACATTTTGGAAAATTT+TGG | + | chr8_2:61354496-61354515 | None:intergenic | 25.0% |
| !! | TGTCAAAATATTAAAGTACC+TGG | - | chr8_2:61354539-61354558 | Msa1259630:intron | 25.0% |
| ! | TTGAATGATAATAGAATCCA+TGG | + | chr8_2:61354586-61354605 | None:intergenic | 25.0% |
| ! | ACAATATCAAATATGGATGA+TGG | - | chr8_2:61354662-61354681 | Msa1259630:intron | 25.0% |
| !! | TTATTGTTGTTATAAGTCTG+TGG | + | chr8_2:61354843-61354862 | None:intergenic | 25.0% |
| !!! | TGCTATTGTTATTTGTGTAA+GGG | + | chr8_2:61354998-61355017 | None:intergenic | 25.0% |
| !!! | TTTTTGATACAGTTGATGAT+AGG | - | chr8_2:61355123-61355142 | Msa1259630:CDS | 25.0% |
| ! | GCAATCATACATTTATTCTA+AGG | - | chr8_2:61355145-61355164 | Msa1259630:CDS | 25.0% |
| !! | ATCATACATTTATTCTAAGG+AGG | - | chr8_2:61355148-61355167 | Msa1259630:CDS | 25.0% |
| !!! | GTGTTTTTGATCTACTTTAA+CGG | + | chr8_2:61355226-61355245 | None:intergenic | 25.0% |
| ATCAACAACTTGAAATTTGC+AGG | - | chr8_2:61354706-61354725 | Msa1259630:intron | 30.0% | |
| TTCACAGTTAGAATTAGGAA+TGG | - | chr8_2:61354770-61354789 | Msa1259630:intron | 30.0% | |
| ATGCTACAAATCCTAATTTG+TGG | - | chr8_2:61354964-61354983 | Msa1259630:CDS | 30.0% | |
| !! | CTGCTATTGTTATTTGTGTA+AGG | + | chr8_2:61354999-61355018 | None:intergenic | 30.0% |
| ! | TATCAGCATTTTCACTACAA+AGG | + | chr8_2:61355422-61355441 | None:intergenic | 30.0% |
| ATCAACAACTTGAAATTTGC+AGG | - | chr8_2:61354706-61354725 | Msa1259630:intron | 30.0% | |
| TTCACAGTTAGAATTAGGAA+TGG | - | chr8_2:61354770-61354789 | Msa1259630:intron | 30.0% | |
| ATGCTACAAATCCTAATTTG+TGG | - | chr8_2:61354964-61354983 | Msa1259630:CDS | 30.0% | |
| !! | CTGCTATTGTTATTTGTGTA+AGG | + | chr8_2:61354999-61355018 | None:intergenic | 30.0% |
| ! | TATCAGCATTTTCACTACAA+AGG | + | chr8_2:61355422-61355441 | None:intergenic | 30.0% |
| !!! | AAATTTTGGGTTGATCTGCA+GGG | + | chr8_2:61354482-61354501 | None:intergenic | 35.0% |
| !!! | AAAATTTTGGGTTGATCTGC+AGG | + | chr8_2:61354483-61354502 | None:intergenic | 35.0% |
| GCAGGCTACAATATCAAATA+TGG | - | chr8_2:61354655-61354674 | Msa1259630:intron | 35.0% | |
| GTGACTTCACAGTTAGAATT+AGG | - | chr8_2:61354765-61354784 | Msa1259630:intron | 35.0% | |
| TTGTTATAAGTCTGTGGTTG+TGG | + | chr8_2:61354837-61354856 | None:intergenic | 35.0% | |
| ATCCTTTGAATCCATGTAGA+GGG | + | chr8_2:61354943-61354962 | None:intergenic | 35.0% | |
| AGCAATGCAACCTCATAAAT+TGG | - | chr8_2:61355025-61355044 | Msa1259630:CDS | 35.0% | |
| ! | TCTGAGGTAACCAATTTATG+AGG | + | chr8_2:61355038-61355057 | None:intergenic | 35.0% |
| TATCTTCAACAACTCCTTGT+TGG | + | chr8_2:61355082-61355101 | None:intergenic | 35.0% | |
| GCTTATGAATCGAGGTAAAT+TGG | - | chr8_2:61355170-61355189 | Msa1259630:CDS | 35.0% | |
| !!! | AAATTTTGGGTTGATCTGCA+GGG | + | chr8_2:61354482-61354501 | None:intergenic | 35.0% |
| !!! | AAAATTTTGGGTTGATCTGC+AGG | + | chr8_2:61354483-61354502 | None:intergenic | 35.0% |
| GCAGGCTACAATATCAAATA+TGG | - | chr8_2:61354655-61354674 | Msa1259630:intron | 35.0% | |
| GTGACTTCACAGTTAGAATT+AGG | - | chr8_2:61354765-61354784 | Msa1259630:intron | 35.0% | |
| TTGTTATAAGTCTGTGGTTG+TGG | + | chr8_2:61354837-61354856 | None:intergenic | 35.0% | |
| ATCCTTTGAATCCATGTAGA+GGG | + | chr8_2:61354943-61354962 | None:intergenic | 35.0% | |
| AGCAATGCAACCTCATAAAT+TGG | - | chr8_2:61355025-61355044 | Msa1259630:CDS | 35.0% | |
| ! | TCTGAGGTAACCAATTTATG+AGG | + | chr8_2:61355038-61355057 | None:intergenic | 35.0% |
| TATCTTCAACAACTCCTTGT+TGG | + | chr8_2:61355082-61355101 | None:intergenic | 35.0% | |
| GCTTATGAATCGAGGTAAAT+TGG | - | chr8_2:61355170-61355189 | Msa1259630:CDS | 35.0% | |
| ! | TTTTCTTCTGACTAGGTTCC+AGG | + | chr8_2:61354560-61354579 | None:intergenic | 40.0% |
| !! | ATGGCTGTTTTCTTCTGACT+AGG | + | chr8_2:61354567-61354586 | None:intergenic | 40.0% |
| TAGTCAGAAGAAAACAGCCA+TGG | - | chr8_2:61354566-61354585 | Msa1259630:intron | 40.0% | |
| GATGTTGTGAGGAAATTTGC+AGG | - | chr8_2:61354637-61354656 | Msa1259630:intron | 40.0% | |
| CTTCATGAGGTAACTGTTGT+TGG | + | chr8_2:61354743-61354762 | None:intergenic | 40.0% | |
| TGTGAAGTCACATCTTCATG+AGG | + | chr8_2:61354756-61354775 | None:intergenic | 40.0% | |
| TGTTGCTGAGATACAGACAA+AGG | + | chr8_2:61354879-61354898 | None:intergenic | 40.0% | |
| GTAATCCACACTATTGCTGT+TGG | + | chr8_2:61354910-61354929 | None:intergenic | 40.0% | |
| CATCCTTTGAATCCATGTAG+AGG | + | chr8_2:61354944-61354963 | None:intergenic | 40.0% | |
| CTAGTTGATCATTGATCCAC+AGG | - | chr8_2:61355253-61355272 | Msa1259630:CDS | 40.0% | |
| TCTCATTTAGTACGACCACT+CGG | + | chr8_2:61355331-61355350 | None:intergenic | 40.0% | |
| ! | TTTTCTTCTGACTAGGTTCC+AGG | + | chr8_2:61354560-61354579 | None:intergenic | 40.0% |
| !! | ATGGCTGTTTTCTTCTGACT+AGG | + | chr8_2:61354567-61354586 | None:intergenic | 40.0% |
| TAGTCAGAAGAAAACAGCCA+TGG | - | chr8_2:61354566-61354585 | Msa1259630:intron | 40.0% | |
| GATGTTGTGAGGAAATTTGC+AGG | - | chr8_2:61354637-61354656 | Msa1259630:intron | 40.0% | |
| CTTCATGAGGTAACTGTTGT+TGG | + | chr8_2:61354743-61354762 | None:intergenic | 40.0% | |
| TGTGAAGTCACATCTTCATG+AGG | + | chr8_2:61354756-61354775 | None:intergenic | 40.0% | |
| TGTTGCTGAGATACAGACAA+AGG | + | chr8_2:61354879-61354898 | None:intergenic | 40.0% | |
| GTAATCCACACTATTGCTGT+TGG | + | chr8_2:61354910-61354929 | None:intergenic | 40.0% | |
| CATCCTTTGAATCCATGTAG+AGG | + | chr8_2:61354944-61354963 | None:intergenic | 40.0% | |
| CTAGTTGATCATTGATCCAC+AGG | - | chr8_2:61355253-61355272 | Msa1259630:CDS | 40.0% | |
| TCTCATTTAGTACGACCACT+CGG | + | chr8_2:61355331-61355350 | None:intergenic | 40.0% | |
| TTGTGTTGTGCCACCCTTAA+GGG | + | chr8_2:61354394-61354413 | None:intergenic | 45.0% | |
| TTGGAATTTGCATGCTGCAC+AGG | + | chr8_2:61354418-61354437 | None:intergenic | 45.0% | |
| CAGGTGTGCACTTTCTTCTT+TGG | + | chr8_2:61354437-61354456 | None:intergenic | 45.0% | |
| CAGGGAAATAAGGAGCTAGA+AGG | + | chr8_2:61354464-61354483 | None:intergenic | 45.0% | |
| GGTTGATCTGCAGGGAAATA+AGG | + | chr8_2:61354474-61354493 | None:intergenic | 45.0% | |
| AGGGATAAGTATCCTGTGCA+TGG | - | chr8_2:61354615-61354634 | Msa1259630:intron | 45.0% | |
| ! | CACACTATTGCTGTTGGAGT+AGG | + | chr8_2:61354904-61354923 | None:intergenic | 45.0% |
| CTACTCCAACAGCAATAGTG+TGG | - | chr8_2:61354902-61354921 | Msa1259630:CDS | 45.0% | |
| CAATAACTCCTCCCTCTACA+TGG | - | chr8_2:61354929-61354948 | Msa1259630:CDS | 45.0% | |
| CTTTGAATCCATGTAGAGGG+AGG | + | chr8_2:61354940-61354959 | None:intergenic | 45.0% | |
| CTCCCTCTACATGGATTCAA+AGG | - | chr8_2:61354938-61354957 | Msa1259630:CDS | 45.0% | |
| CCTTGTTGGACAGATGATAG+TGG | + | chr8_2:61355068-61355087 | None:intergenic | 45.0% | |
| CCACTATCATCTGTCCAACA+AGG | - | chr8_2:61355065-61355084 | Msa1259630:CDS | 45.0% | |
| CTAAGGAGGCTTATGAATCG+AGG | - | chr8_2:61355162-61355181 | Msa1259630:CDS | 45.0% | |
| ACAAGATTACCTCTGGTCGA+GGG | + | chr8_2:61355281-61355300 | None:intergenic | 45.0% | |
| GACCAGAGGTAATCTTGTCA+GGG | - | chr8_2:61355283-61355302 | Msa1259630:CDS | 45.0% | |
| ACCAGAGGTAATCTTGTCAG+GGG | - | chr8_2:61355284-61355303 | Msa1259630:CDS | 45.0% | |
| TCATTTAGTACGACCACTCG+GGG | + | chr8_2:61355329-61355348 | None:intergenic | 45.0% | |
| CTCATTTAGTACGACCACTC+GGG | + | chr8_2:61355330-61355349 | None:intergenic | 45.0% | |
| GTACATTTCTGCCTGCAGTT+CGG | - | chr8_2:61355452-61355471 | Msa1259630:CDS | 45.0% | |
| TTGTGTTGTGCCACCCTTAA+GGG | + | chr8_2:61354394-61354413 | None:intergenic | 45.0% | |
| TTGGAATTTGCATGCTGCAC+AGG | + | chr8_2:61354418-61354437 | None:intergenic | 45.0% | |
| CAGGTGTGCACTTTCTTCTT+TGG | + | chr8_2:61354437-61354456 | None:intergenic | 45.0% | |
| CAGGGAAATAAGGAGCTAGA+AGG | + | chr8_2:61354464-61354483 | None:intergenic | 45.0% | |
| GGTTGATCTGCAGGGAAATA+AGG | + | chr8_2:61354474-61354493 | None:intergenic | 45.0% | |
| AGGGATAAGTATCCTGTGCA+TGG | - | chr8_2:61354615-61354634 | Msa1259630:intron | 45.0% | |
| ! | CACACTATTGCTGTTGGAGT+AGG | + | chr8_2:61354904-61354923 | None:intergenic | 45.0% |
| CTACTCCAACAGCAATAGTG+TGG | - | chr8_2:61354902-61354921 | Msa1259630:CDS | 45.0% | |
| CAATAACTCCTCCCTCTACA+TGG | - | chr8_2:61354929-61354948 | Msa1259630:CDS | 45.0% | |
| CTTTGAATCCATGTAGAGGG+AGG | + | chr8_2:61354940-61354959 | None:intergenic | 45.0% | |
| CTCCCTCTACATGGATTCAA+AGG | - | chr8_2:61354938-61354957 | Msa1259630:CDS | 45.0% | |
| CCTTGTTGGACAGATGATAG+TGG | + | chr8_2:61355068-61355087 | None:intergenic | 45.0% | |
| CCACTATCATCTGTCCAACA+AGG | - | chr8_2:61355065-61355084 | Msa1259630:CDS | 45.0% | |
| CTAAGGAGGCTTATGAATCG+AGG | - | chr8_2:61355162-61355181 | Msa1259630:CDS | 45.0% | |
| ACAAGATTACCTCTGGTCGA+GGG | + | chr8_2:61355281-61355300 | None:intergenic | 45.0% | |
| GACCAGAGGTAATCTTGTCA+GGG | - | chr8_2:61355283-61355302 | Msa1259630:CDS | 45.0% | |
| ACCAGAGGTAATCTTGTCAG+GGG | - | chr8_2:61355284-61355303 | Msa1259630:CDS | 45.0% | |
| TCATTTAGTACGACCACTCG+GGG | + | chr8_2:61355329-61355348 | None:intergenic | 45.0% | |
| CTCATTTAGTACGACCACTC+GGG | + | chr8_2:61355330-61355349 | None:intergenic | 45.0% | |
| GTACATTTCTGCCTGCAGTT+CGG | - | chr8_2:61355452-61355471 | Msa1259630:CDS | 45.0% | |
| CTTGTGTTGTGCCACCCTTA+AGG | + | chr8_2:61354395-61354414 | None:intergenic | 50.0% | |
| !! | TAAGGAGCTAGAAGGCACTC+AGG | + | chr8_2:61354456-61354475 | None:intergenic | 50.0% |
| TCCTGTGCATGGATGTTGTG+AGG | - | chr8_2:61354626-61354645 | Msa1259630:intron | 50.0% | |
| TCCTCACAACATCCATGCAC+AGG | + | chr8_2:61354630-61354649 | None:intergenic | 50.0% | |
| ! | AAAGAGTGCGAGCGCATTGA+AGG | + | chr8_2:61354805-61354824 | None:intergenic | 50.0% |
| !! | GGGCACTGAAGCCACAAATT+AGG | + | chr8_2:61354978-61354997 | None:intergenic | 50.0% |
| GACAAGATTACCTCTGGTCG+AGG | + | chr8_2:61355282-61355301 | None:intergenic | 50.0% | |
| CGACCAGAGGTAATCTTGTC+AGG | - | chr8_2:61355282-61355301 | Msa1259630:CDS | 50.0% | |
| ACCCCTGACAAGATTACCTC+TGG | + | chr8_2:61355288-61355307 | None:intergenic | 50.0% | |
| CTTGTGTTGTGCCACCCTTA+AGG | + | chr8_2:61354395-61354414 | None:intergenic | 50.0% | |
| !! | TAAGGAGCTAGAAGGCACTC+AGG | + | chr8_2:61354456-61354475 | None:intergenic | 50.0% |
| TCCTGTGCATGGATGTTGTG+AGG | - | chr8_2:61354626-61354645 | Msa1259630:intron | 50.0% | |
| TCCTCACAACATCCATGCAC+AGG | + | chr8_2:61354630-61354649 | None:intergenic | 50.0% | |
| ! | AAAGAGTGCGAGCGCATTGA+AGG | + | chr8_2:61354805-61354824 | None:intergenic | 50.0% |
| !! | GGGCACTGAAGCCACAAATT+AGG | + | chr8_2:61354978-61354997 | None:intergenic | 50.0% |
| GACAAGATTACCTCTGGTCG+AGG | + | chr8_2:61355282-61355301 | None:intergenic | 50.0% | |
| CGACCAGAGGTAATCTTGTC+AGG | - | chr8_2:61355282-61355301 | Msa1259630:CDS | 50.0% | |
| ACCCCTGACAAGATTACCTC+TGG | + | chr8_2:61355288-61355307 | None:intergenic | 50.0% | |
| !! | TGATAGTGGCTGCGACTCTG+AGG | + | chr8_2:61355054-61355073 | None:intergenic | 55.0% |
| CCACTCGGGGAGGTTAGAAT+TGG | + | chr8_2:61355316-61355335 | None:intergenic | 55.0% | |
| CCAATTCTAACCTCCCCGAG+TGG | - | chr8_2:61355313-61355332 | Msa1259630:CDS | 55.0% | |
| TTTAGTACGACCACTCGGGG+AGG | + | chr8_2:61355326-61355345 | None:intergenic | 55.0% | |
| CAGCGATTCTTCCGAACTGC+AGG | + | chr8_2:61355466-61355485 | None:intergenic | 55.0% | |
| !! | TGATAGTGGCTGCGACTCTG+AGG | + | chr8_2:61355054-61355073 | None:intergenic | 55.0% |
| CCACTCGGGGAGGTTAGAAT+TGG | + | chr8_2:61355316-61355335 | None:intergenic | 55.0% | |
| CCAATTCTAACCTCCCCGAG+TGG | - | chr8_2:61355313-61355332 | Msa1259630:CDS | 55.0% | |
| TTTAGTACGACCACTCGGGG+AGG | + | chr8_2:61355326-61355345 | None:intergenic | 55.0% | |
| CAGCGATTCTTCCGAACTGC+AGG | + | chr8_2:61355466-61355485 | None:intergenic | 55.0% | |
| CCTCTGGTCGAGGGAACCTG+TGG | + | chr8_2:61355272-61355291 | None:intergenic | 65.0% | |
| CCACAGGTTCCCTCGACCAG+AGG | - | chr8_2:61355269-61355288 | Msa1259630:CDS | 65.0% | |
| CCTCTGGTCGAGGGAACCTG+TGG | + | chr8_2:61355272-61355291 | None:intergenic | 65.0% | |
| CCACAGGTTCCCTCGACCAG+AGG | - | chr8_2:61355269-61355288 | Msa1259630:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 61354387 | 61355572 | 61354387 | ID=Msa1259630;Name=Msa1259630 |
| chr8_2 | mRNA | 61354387 | 61355572 | 61354387 | ID=Msa1259630-mRNA-1;Parent=Msa1259630;Name=Msa1259630-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|0|0|1|1|1|2|0|299 |
| chr8_2 | exon | 61354776 | 61355572 | 61354776 | ID=Msa1259630-mRNA-1:exon:19304;Parent=Msa1259630-mRNA-1 |
| chr8_2 | exon | 61354387 | 61354489 | 61354387 | ID=Msa1259630-mRNA-1:exon:19303;Parent=Msa1259630-mRNA-1 |
| chr8_2 | CDS | 61354776 | 61355572 | 61354776 | ID=Msa1259630-mRNA-1:cds;Parent=Msa1259630-mRNA-1 |
| chr8_2 | CDS | 61354387 | 61354489 | 61354387 | ID=Msa1259630-mRNA-1:cds;Parent=Msa1259630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
