AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1262070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262070 MtrunA17_Chr4g0063361 83.607 244 40 0 1 244 1 244 8.34e-155 434
Msa1262070 MtrunA17_Chr4g0063401 60.769 260 80 6 1 246 1 252 1.40e-112 327
Msa1262070 MtrunA17_Chr4g0063391 52.066 242 83 3 1 242 1 209 9.75e-84 253
Msa1262070 MtrunA17_Chr4g0063371 41.411 326 90 6 1 321 1 230 3.96e-72 223
Msa1262070 MtrunA17_Chr3g0098251 47.644 191 80 4 51 241 7 177 2.87e-57 184
Msa1262070 MtrunA17_Chr8g0360671 45.270 148 58 4 105 250 41 167 4.33e-35 125
Msa1262070 MtrunA17_Chr8g0366501 29.236 301 149 7 77 322 83 374 3.50e-29 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262070 AT3G19360.1 36.697 327 148 6 49 325 59 376 1.49e-64 209
Msa1262070 AT3G19360.2 36.697 327 148 6 49 325 59 376 1.49e-64 209
Msa1262070 AT1G32360.1 27.703 296 156 5 82 322 90 382 2.15e-25 105
Msa1262070 AT2G35430.1 27.638 199 116 5 138 322 66 250 5.20e-15 74.3

Find 89 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAGATCAATCGTGTTTA+TGG 0.098083 8_2:-64643155 Msa1262070:CDS
GACAGCAGCAGCTGAGAAAT+TGG 0.269527 8_2:-64645196 Msa1262070:CDS
TCCTTTGCCATAAGTCTTGC+AGG 0.273145 8_2:+64643221 None:intergenic
ATGAGGATCCTGCTAAGTTT+AGG 0.284721 8_2:-64645079 Msa1262070:CDS
CTAACTAGCTCCTGCCAATT+TGG 0.294724 8_2:+64645233 None:intergenic
TCTATAAGCATTGGGGCTAT+TGG 0.301858 8_2:-64645026 Msa1262070:CDS
ATTGGTTCTTCAAAGTCATT+TGG 0.320226 8_2:-64645008 Msa1262070:CDS
GATGGAGGAAGAACCATCTT+TGG 0.330810 8_2:+64645377 None:intergenic
TGAGGATCCTGCTAAGTTTA+GGG 0.331108 8_2:-64645078 Msa1262070:CDS
AATGGTGAGAAATGTTTCTT+TGG 0.334123 8_2:-64645122 Msa1262070:CDS
CTAAGTTTAGGGATCATTCT+TGG 0.335825 8_2:-64645067 Msa1262070:CDS
CACCTTCCCTGCAAGACTTA+TGG 0.338047 8_2:-64643228 Msa1262070:CDS
TTGCGGCGGTGACATCTTAC+AGG 0.346131 8_2:+64645556 None:intergenic
AGCCAGCTAGGGGCACTTAT+TGG 0.348926 8_2:-64644962 Msa1262070:CDS
ACTTCACGAAAAGCCTTAAC+AGG 0.358557 8_2:-64643278 Msa1262070:CDS
TTGGTTCTTCAAAGTCATTT+GGG 0.358694 8_2:-64645007 Msa1262070:CDS
TCAATCGTGTTTATGGTGAT+TGG 0.369324 8_2:-64643148 Msa1262070:CDS
ATCGAGGGAGATGTCCCTTT+GGG 0.370733 8_2:-64644911 Msa1262070:CDS
ATCAACCCCATTCCTTCAAA+AGG 0.390230 8_2:-64645448 Msa1262070:CDS
TAAGACTGCCACCACCAAAT+TGG 0.391036 8_2:-64645247 Msa1262070:CDS
ACGTGGTCTGCTCTGTTTGA+TGG 0.398666 8_2:+64645359 None:intergenic
AGTGTTCTTCTATAAGCATT+GGG 0.403399 8_2:-64645034 Msa1262070:CDS
CTAAGAAGATGCATTTACTT+TGG 0.420995 8_2:-64643187 Msa1262070:CDS
GGGCTGTGGACAAGCCAGCT+AGG 0.423889 8_2:-64644974 Msa1262070:CDS
AAAGAAACATTTCTCACCAT+TGG 0.440730 8_2:+64645124 None:intergenic
TTACAGGTTGGAATATTGTC+TGG 0.446583 8_2:+64645572 None:intergenic
GGCTGTGGACAAGCCAGCTA+GGG 0.459838 8_2:-64644973 Msa1262070:CDS
AGTCATTTGGGGATGGCACT+AGG 0.462888 8_2:-64644995 Msa1262070:CDS
ATATGATGTTCAAATTATAG+TGG 0.472827 8_2:-64644704 Msa1262070:CDS
TGCCATAAGTCTTGCAGGGA+AGG 0.474061 8_2:+64643226 None:intergenic
CATCGAGGGAGATGTCCCTT+TGG 0.474158 8_2:-64644912 Msa1262070:CDS
TTCCAATAAGTGCCCCTAGC+TGG 0.475908 8_2:+64644960 None:intergenic
ACAGCAGCAGCTGAGAAATT+GGG 0.480050 8_2:-64645195 Msa1262070:CDS
GATATGCCCTCCTCCAAAGA+TGG 0.484001 8_2:-64645390 Msa1262070:CDS
TGGGGATGGCACTAGGGCTG+TGG 0.491393 8_2:-64644988 Msa1262070:CDS
GTTTCTACAAGTACCAAGTC+TGG 0.493005 8_2:+64645307 None:intergenic
AGTCAGTTCTCCACTGCACT+TGG 0.503719 8_2:+64643092 None:intergenic
GAATGATCCCTAAACTTAGC+AGG 0.506220 8_2:+64645071 None:intergenic
AAAGATTGCAACTTTGCTCA+TGG 0.506227 8_2:-64645281 Msa1262070:CDS
TCCACTGCACTTGGCAAGGT+GGG 0.506459 8_2:+64643101 None:intergenic
TTCACAAGATGAAGCTATGC+AGG 0.506731 8_2:-64645154 Msa1262070:CDS
CAATTTGCTCATGGAGAAGC+AGG 0.507401 8_2:-64644879 Msa1262070:intron
AGACTAAGACGTGTTTCCAT+TGG 0.510007 8_2:-64644938 Msa1262070:CDS
ATCTCCCTCGATGCTGCCAA+TGG 0.511208 8_2:+64644922 None:intergenic
TGGTAGGTGCATATGCATCA+TGG 0.515067 8_2:+64643249 None:intergenic
GAGTGTTCTTCTATAAGCAT+TGG 0.515583 8_2:-64645035 Msa1262070:CDS
AGACTTGGTACTTGTAGAAA+CGG 0.521320 8_2:-64645305 Msa1262070:CDS
AAGACTTATGGCAAAGGAAG+AGG 0.525073 8_2:-64643216 Msa1262070:CDS
ATCATGGGCAGAACCTGTTA+AGG 0.525153 8_2:+64643265 None:intergenic
GAGGACTGTCAATTTGCTCA+TGG 0.526443 8_2:-64644888 Msa1262070:CDS
GTTTCCATTGGCAGCATCGA+GGG 0.527680 8_2:-64644926 Msa1262070:CDS
TATGGCAAAGGAAGAGGAGC+AGG 0.527945 8_2:-64643210 Msa1262070:CDS
TCTTCAAAGTCATTTGGGGA+TGG 0.542249 8_2:-64645002 Msa1262070:CDS
TCCCACCTTGCCAAGTGCAG+TGG 0.542507 8_2:-64643102 Msa1262070:CDS
GTCATTTGGGGATGGCACTA+GGG 0.548609 8_2:-64644994 Msa1262070:CDS
AGCTCCTGCCAATTTGGTGG+TGG 0.549332 8_2:+64645239 None:intergenic
ACTAGCTCCTGCCAATTTGG+TGG 0.554201 8_2:+64645236 None:intergenic
AGGAGGGCATATCAAAGCAT+TGG 0.555491 8_2:+64645400 None:intergenic
ACTGCCACCACCAAATTGGC+AGG 0.556769 8_2:-64645243 Msa1262070:CDS
GGAAGAACCATCTTTGGAGG+AGG 0.559899 8_2:+64645383 None:intergenic
AATTGACAGTCCTCCCCAAA+GGG 0.560613 8_2:+64644897 None:intergenic
GTTCTCCACTGCACTTGGCA+AGG 0.561112 8_2:+64643097 None:intergenic
TCAACCCCATTCCTTCAAAA+GGG 0.562069 8_2:-64645447 Msa1262070:CDS
GGAGGAAGAACCATCTTTGG+AGG 0.566069 8_2:+64645380 None:intergenic
GGTAGGTGCATATGCATCAT+GGG 0.568261 8_2:+64643250 None:intergenic
ATCTGTAATAAGTCCAGACT+TGG 0.576422 8_2:-64645320 Msa1262070:CDS
CCTTTGCCATAAGTCTTGCA+GGG 0.581829 8_2:+64643222 None:intergenic
CATAAGTCTTGCAGGGAAGG+TGG 0.582500 8_2:+64643229 None:intergenic
CTCCACTGCACTTGGCAAGG+TGG 0.585446 8_2:+64643100 None:intergenic
CCCTGCAAGACTTATGGCAA+AGG 0.589645 8_2:-64643222 Msa1262070:CDS
AGTCTTGCAGGGAAGGTGGT+AGG 0.597135 8_2:+64643233 None:intergenic
GGCGGTGACATCTTACAGGT+TGG 0.597501 8_2:+64645560 None:intergenic
TGGTTCTTCAAAGTCATTTG+GGG 0.598253 8_2:-64645006 Msa1262070:CDS
GGCAAGGTGGGAAGAACAAG+AGG 0.612688 8_2:+64643113 None:intergenic
CTAGTTAGTCCTCGCAGCGA+CGG 0.619159 8_2:-64645218 Msa1262070:CDS
AAATTGACAGTCCTCCCCAA+AGG 0.626382 8_2:+64644896 None:intergenic
CTGTTTGATGTAGTGAATTG+CGG 0.628943 8_2:+64645539 None:intergenic
GAAGAACCATCTTTGGAGGA+GGG 0.630074 8_2:+64645384 None:intergenic
AGGGAGATGTCCCTTTGGGG+AGG 0.650401 8_2:-64644907 Msa1262070:CDS
CTATGCAGGATGTATTCCAA+TGG 0.653635 8_2:-64645140 Msa1262070:CDS
GTGTTCTTCTATAAGCATTG+GGG 0.654358 8_2:-64645033 Msa1262070:CDS
TGGTCTGCTCTGTTTGATGG+AGG 0.659565 8_2:+64645362 None:intergenic
GCTGTGGACAAGCCAGCTAG+GGG 0.660887 8_2:-64644972 Msa1262070:CDS
TCGAGGGAGATGTCCCTTTG+GGG 0.664726 8_2:-64644910 Msa1262070:CDS
TGTTTCCATTGGCAGCATCG+AGG 0.674096 8_2:-64644927 Msa1262070:CDS
GCAGCAGCTGAGAAATTGGG+AGG 0.680326 8_2:-64645192 Msa1262070:CDS
TTTGATGTAGTGAATTGCGG+CGG 0.699237 8_2:+64645542 None:intergenic
TACGAGTCTTGTAGAAAACG+TGG 0.701748 8_2:+64645342 None:intergenic
AATATTGTCTGGAAGAACCA+TGG 0.708668 8_2:+64645583 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATCAATAATCAAAATA+GGG + chr8_2:64643857-64643876 None:intergenic 15.0%
!!! ATCCATTTACAAATTTTTTA+TGG + chr8_2:64644466-64644485 None:intergenic 15.0%
!!! TTAATCATCTCAATTTTATA+AGG - chr8_2:64644684-64644703 Msa1262070:intron 15.0%
!! AAAAGATGAATATAACATAA+TGG + chr8_2:64644975-64644994 None:intergenic 15.0%
!! CAAGCAATATATATAAAAAA+TGG + chr8_2:64645231-64645250 None:intergenic 15.0%
!!! ATTTTACAATTTAAACTGTT+TGG - chr8_2:64645302-64645321 Msa1262070:CDS 15.0%
!!! TGAAAGTTTTCATTTTTCAT+TGG + chr8_2:64643190-64643209 None:intergenic 20.0%
!! AATAATCAAAATAGGGAAAA+GGG + chr8_2:64643850-64643869 None:intergenic 20.0%
!! CCAAAATCAATAATCAAAAT+AGG + chr8_2:64643858-64643877 None:intergenic 20.0%
!!! CCTATTTTGATTATTGATTT+TGG - chr8_2:64643855-64643874 Msa1262070:intron 20.0%
!! ATATGATGTTCAAATTATAG+TGG - chr8_2:64643971-64643990 Msa1262070:intron 20.0%
!! AAAGATAAAATAATCACTAG+TGG + chr8_2:64644247-64644266 None:intergenic 20.0%
!! AGCCATAAAAAATTTGTAAA+TGG - chr8_2:64644461-64644480 Msa1262070:intron 20.0%
!!! GTGTTTATATGTTGTATTTT+TGG - chr8_2:64644519-64644538 Msa1262070:intron 20.0%
!! TCGGATACAAAAAAAAAATT+AGG - chr8_2:64644861-64644880 Msa1262070:intron 20.0%
!!! TAAGTGTTATTTTCTTCATG+AGG - chr8_2:64643579-64643598 Msa1262070:intron 25.0%
! CAATAATCAAAATAGGGAAA+AGG + chr8_2:64643851-64643870 None:intergenic 25.0%
! AAAACTGTGTTGTCAAATAT+CGG - chr8_2:64644081-64644100 Msa1262070:intron 25.0%
! GATATGTAAACCTTAGATAA+AGG + chr8_2:64644387-64644406 None:intergenic 25.0%
!! TACATATCAGTGTTGTTAAA+TGG - chr8_2:64644399-64644418 Msa1262070:intron 25.0%
! TCAAATAGAAATTCCTAATG+TGG + chr8_2:64644632-64644651 None:intergenic 25.0%
! AAGGCATGATTACTATTAAA+TGG - chr8_2:64644703-64644722 Msa1262070:CDS 25.0%
!!! TGACTGAAATTTTAGTAGTT+TGG - chr8_2:64644750-64644769 Msa1262070:intron 25.0%
!! TATATGTCGATGGATATTTT+GGG - chr8_2:64644898-64644917 Msa1262070:CDS 25.0%
!! TATTTCTATGTATTGGATCA+CGG + chr8_2:64644931-64644950 None:intergenic 25.0%
!! TCACATGTATTTCTATGTAT+TGG + chr8_2:64644938-64644957 None:intergenic 25.0%
! TGAGTTAGACATCAAATTAT+AGG + chr8_2:64645038-64645057 None:intergenic 25.0%
! CATATTTGACTGCAATATTA+AGG + chr8_2:64645121-64645140 None:intergenic 25.0%
! CTTAATATTGCAGTCAAATA+TGG - chr8_2:64645119-64645138 Msa1262070:CDS 25.0%
!! TCATTTTTCATTGGAAATCG+AGG + chr8_2:64643181-64643200 None:intergenic 30.0%
AAAGAAACATTTCTCACCAT+TGG + chr8_2:64643554-64643573 None:intergenic 30.0%
AATGGTGAGAAATGTTTCTT+TGG - chr8_2:64643553-64643572 Msa1262070:intron 30.0%
AGTGTTCTTCTATAAGCATT+GGG - chr8_2:64643641-64643660 Msa1262070:intron 30.0%
! ATTGGTTCTTCAAAGTCATT+TGG - chr8_2:64643667-64643686 Msa1262070:intron 30.0%
! TTGGTTCTTCAAAGTCATTT+GGG - chr8_2:64643668-64643687 Msa1262070:intron 30.0%
ATCATATAATTCTGCTGTAC+TGG - chr8_2:64643882-64643901 Msa1262070:intron 30.0%
! GCGTATGATATTTTCATGTT+TGG - chr8_2:64644019-64644038 Msa1262070:intron 30.0%
TCTTTGTGTTCAAATTTCCT+TGG - chr8_2:64644262-64644281 Msa1262070:intron 30.0%
TGACAACTGCTATGTTTATA+TGG - chr8_2:64644313-64644332 Msa1262070:intron 30.0%
TGATAACACTCCTTTATCTA+AGG - chr8_2:64644374-64644393 Msa1262070:intron 30.0%
CTTTATAGCTGATAACAGAA+AGG + chr8_2:64644795-64644814 None:intergenic 30.0%
TGTTATCAGCTATAAAGCAT+GGG - chr8_2:64644798-64644817 Msa1262070:intron 30.0%
! GTATATGTCGATGGATATTT+TGG - chr8_2:64644897-64644916 Msa1262070:CDS 30.0%
!! CTAACTCATCAATAGTTTTG+AGG - chr8_2:64645050-64645069 Msa1262070:CDS 30.0%
CAATATTAAGGATTGTGACA+TGG + chr8_2:64645109-64645128 None:intergenic 30.0%
!!! ACGTTGAGGTTTTTTAATTG+AGG + chr8_2:64645184-64645203 None:intergenic 30.0%
CTAAGAAGATGCATTTACTT+TGG - chr8_2:64645488-64645507 Msa1262070:CDS 30.0%
AACAAGATCAATCGTGTTTA+TGG - chr8_2:64645520-64645539 Msa1262070:CDS 30.0%
! TTACAGGTTGGAATATTGTC+TGG + chr8_2:64643106-64643125 None:intergenic 35.0%
CTGTTTGATGTAGTGAATTG+CGG + chr8_2:64643139-64643158 None:intergenic 35.0%
! AGAAATTCTTGCCCTTTTGA+AGG + chr8_2:64643242-64643261 None:intergenic 35.0%
ATCTGTAATAAGTCCAGACT+TGG - chr8_2:64643355-64643374 Msa1262070:intron 35.0%
! AGACTTGGTACTTGTAGAAA+CGG - chr8_2:64643370-64643389 Msa1262070:intron 35.0%
AAAGATTGCAACTTTGCTCA+TGG - chr8_2:64643394-64643413 Msa1262070:intron 35.0%
CTAAGTTTAGGGATCATTCT+TGG - chr8_2:64643608-64643627 Msa1262070:intron 35.0%
GAGTGTTCTTCTATAAGCAT+TGG - chr8_2:64643640-64643659 Msa1262070:intron 35.0%
! GTGTTCTTCTATAAGCATTG+GGG - chr8_2:64643642-64643661 Msa1262070:intron 35.0%
TGGTTCTTCAAAGTCATTTG+GGG - chr8_2:64643669-64643688 Msa1262070:intron 35.0%
GTGGCTTAAAATTGCACATT+TGG - chr8_2:64644176-64644195 Msa1262070:intron 35.0%
CAACTGCTATGTTTATATGG+CGG - chr8_2:64644316-64644335 Msa1262070:intron 35.0%
!! TTTATATGGCGGATTTCAGT+TGG - chr8_2:64644327-64644346 Msa1262070:intron 35.0%
GGATGGTTAAATATACTGGA+AGG + chr8_2:64644545-64644564 None:intergenic 35.0%
TGGAGGATGGTTAAATATAC+TGG + chr8_2:64644549-64644568 None:intergenic 35.0%
GTATATTTAACCATCCTCCA+TGG - chr8_2:64644549-64644568 Msa1262070:intron 35.0%
CTGTTATCAGCTATAAAGCA+TGG - chr8_2:64644797-64644816 Msa1262070:intron 35.0%
!! TAGGTATCGAAATTGCTTGT+CGG - chr8_2:64644842-64644861 Msa1262070:intron 35.0%
AAAATTAGGATGCTGCACTT+TGG - chr8_2:64644875-64644894 Msa1262070:intron 35.0%
!! TCCAAACCTGTTTTTCCTTT+TGG - chr8_2:64644994-64645013 Msa1262070:CDS 35.0%
ACCAAAAGGAAAAACAGGTT+TGG + chr8_2:64644998-64645017 None:intergenic 35.0%
CAGAAACCAAAAGGAAAAAC+AGG + chr8_2:64645003-64645022 None:intergenic 35.0%
ATGAGTCAGAGTATGATACA+TGG + chr8_2:64645331-64645350 None:intergenic 35.0%
TCAATCGTGTTTATGGTGAT+TGG - chr8_2:64645527-64645546 Msa1262070:CDS 35.0%
TTTGATGTAGTGAATTGCGG+CGG + chr8_2:64643136-64643155 None:intergenic 40.0%
ATCAACCCCATTCCTTCAAA+AGG - chr8_2:64643227-64643246 Msa1262070:CDS 40.0%
TCAACCCCATTCCTTCAAAA+GGG - chr8_2:64643228-64643247 Msa1262070:CDS 40.0%
!! TCTTGCCCTTTTGAAGGAAT+GGG + chr8_2:64643236-64643255 None:intergenic 40.0%
!! TTCTTGCCCTTTTGAAGGAA+TGG + chr8_2:64643237-64643256 None:intergenic 40.0%
! TACGAGTCTTGTAGAAAACG+TGG + chr8_2:64643336-64643355 None:intergenic 40.0%
GTTTCTACAAGTACCAAGTC+TGG + chr8_2:64643371-64643390 None:intergenic 40.0%
TTCACAAGATGAAGCTATGC+AGG - chr8_2:64643521-64643540 Msa1262070:intron 40.0%
CTATGCAGGATGTATTCCAA+TGG - chr8_2:64643535-64643554 Msa1262070:intron 40.0%
ATGAGGATCCTGCTAAGTTT+AGG - chr8_2:64643596-64643615 Msa1262070:intron 40.0%
TGAGGATCCTGCTAAGTTTA+GGG - chr8_2:64643597-64643616 Msa1262070:intron 40.0%
GAATGATCCCTAAACTTAGC+AGG + chr8_2:64643607-64643626 None:intergenic 40.0%
!! TCTATAAGCATTGGGGCTAT+TGG - chr8_2:64643649-64643668 Msa1262070:intron 40.0%
TCTTCAAAGTCATTTGGGGA+TGG - chr8_2:64643673-64643692 Msa1262070:intron 40.0%
AGACTAAGACGTGTTTCCAT+TGG - chr8_2:64643737-64643756 Msa1262070:intron 40.0%
ACTGTGTTGTCAAATATCGG+CGG - chr8_2:64644084-64644103 Msa1262070:intron 40.0%
! CGGTCAAATGCCAGATTTTT+AGG - chr8_2:64644104-64644123 Msa1262070:intron 40.0%
CATGGTGCTTCCTAAAAATC+TGG + chr8_2:64644117-64644136 None:intergenic 40.0%
!!! CAGATTTTTAGGAAGCACCA+TGG - chr8_2:64644115-64644134 Msa1262070:intron 40.0%
ATATCAATGCGTTGCTACCA+AGG + chr8_2:64644282-64644301 None:intergenic 40.0%
AAACACACCATTGTGACCTA+TGG + chr8_2:64644505-64644524 None:intergenic 40.0%
! ATTTTCGTGTCAGCCACATT+AGG - chr8_2:64644616-64644635 Msa1262070:intron 40.0%
TGCACTTTGGTATATGTCGA+TGG - chr8_2:64644888-64644907 Msa1262070:CDS 40.0%
!!! TTCCTTTTGGTTTCTGCTGA+TGG - chr8_2:64645007-64645026 Msa1262070:CDS 40.0%
!!! TCCTTTTGGTTTCTGCTGAT+GGG - chr8_2:64645008-64645027 Msa1262070:CDS 40.0%
AGGTACATTGCTCATTTGCA+AGG - chr8_2:64645070-64645089 Msa1262070:CDS 40.0%
! GTTAAGGCTTTTCGTGAAGT+TGG + chr8_2:64645397-64645416 None:intergenic 40.0%
ACTTCACGAAAAGCCTTAAC+AGG - chr8_2:64645397-64645416 Msa1262070:CDS 40.0%
AAGACTTATGGCAAAGGAAG+AGG - chr8_2:64645459-64645478 Msa1262070:CDS 40.0%
!! CTTGCCCTTTTGAAGGAATG+GGG + chr8_2:64643235-64643254 None:intergenic 45.0%
! AGGAGGGCATATCAAAGCAT+TGG + chr8_2:64643278-64643297 None:intergenic 45.0%
! GAAGAACCATCTTTGGAGGA+GGG + chr8_2:64643294-64643313 None:intergenic 45.0%
GATGGAGGAAGAACCATCTT+TGG + chr8_2:64643301-64643320 None:intergenic 45.0%
TAAGACTGCCACCACCAAAT+TGG - chr8_2:64643428-64643447 Msa1262070:intron 45.0%
CTAACTAGCTCCTGCCAATT+TGG + chr8_2:64643445-64643464 None:intergenic 45.0%
ACAGCAGCAGCTGAGAAATT+GGG - chr8_2:64643480-64643499 Msa1262070:intron 45.0%
AATTGACAGTCCTCCCCAAA+GGG + chr8_2:64643781-64643800 None:intergenic 45.0%
AAATTGACAGTCCTCCCCAA+AGG + chr8_2:64643782-64643801 None:intergenic 45.0%
! GAGGACTGTCAATTTGCTCA+TGG - chr8_2:64643787-64643806 Msa1262070:intron 45.0%
! CAATTTGCTCATGGAGAAGC+AGG - chr8_2:64643796-64643815 Msa1262070:intron 45.0%
CATCCATCACAAATTGGCCA+TGG + chr8_2:64644135-64644154 None:intergenic 45.0%
! CATGGCCAATTTGTGATGGA+TGG - chr8_2:64644133-64644152 Msa1262070:intron 45.0%
CATCGCCATCCATCACAAAT+TGG + chr8_2:64644141-64644160 None:intergenic 45.0%
TAAGCCACCATAAAACGCCA+TGG + chr8_2:64644164-64644183 None:intergenic 45.0%
GTGTTTACGATGACAGATGG+TGG + chr8_2:64644206-64644225 None:intergenic 45.0%
CCAGTGTTTACGATGACAGA+TGG + chr8_2:64644209-64644228 None:intergenic 45.0%
CCATCTGTCATCGTAAACAC+TGG - chr8_2:64644206-64644225 Msa1262070:intron 45.0%
GTGTTATCAGTAGCAGATGG+TGG + chr8_2:64644363-64644382 None:intergenic 45.0%
! GGAGTGTTATCAGTAGCAGA+TGG + chr8_2:64644366-64644385 None:intergenic 45.0%
AGTGCCAAGACAGAATAGTG+TGG - chr8_2:64644423-64644442 Msa1262070:intron 45.0%
TCTGCCACACTATTCTGTCT+TGG + chr8_2:64644430-64644449 None:intergenic 45.0%
ACCCATCAGCAGAAACCAAA+AGG + chr8_2:64645012-64645031 None:intergenic 45.0%
CAATTGTTGCAGAGACGTTG+AGG + chr8_2:64645198-64645217 None:intergenic 45.0%
ATCATGGGCAGAACCTGTTA+AGG + chr8_2:64645413-64645432 None:intergenic 45.0%
GGTAGGTGCATATGCATCAT+GGG + chr8_2:64645428-64645447 None:intergenic 45.0%
TGGTAGGTGCATATGCATCA+TGG + chr8_2:64645429-64645448 None:intergenic 45.0%
CCTTTGCCATAAGTCTTGCA+GGG + chr8_2:64645456-64645475 None:intergenic 45.0%
TCCTTTGCCATAAGTCTTGC+AGG + chr8_2:64645457-64645476 None:intergenic 45.0%
GATATGCCCTCCTCCAAAGA+TGG - chr8_2:64643285-64643304 Msa1262070:CDS 50.0%
! GGAAGAACCATCTTTGGAGG+AGG + chr8_2:64643295-64643314 None:intergenic 50.0%
GGAGGAAGAACCATCTTTGG+AGG + chr8_2:64643298-64643317 None:intergenic 50.0%
TGGTCTGCTCTGTTTGATGG+AGG + chr8_2:64643316-64643335 None:intergenic 50.0%
ACGTGGTCTGCTCTGTTTGA+TGG + chr8_2:64643319-64643338 None:intergenic 50.0%
ACTAGCTCCTGCCAATTTGG+TGG + chr8_2:64643442-64643461 None:intergenic 50.0%
GACAGCAGCAGCTGAGAAAT+TGG - chr8_2:64643479-64643498 Msa1262070:intron 50.0%
AGTCATTTGGGGATGGCACT+AGG - chr8_2:64643680-64643699 Msa1262070:intron 50.0%
GTCATTTGGGGATGGCACTA+GGG - chr8_2:64643681-64643700 Msa1262070:intron 50.0%
TTCCAATAAGTGCCCCTAGC+TGG + chr8_2:64643718-64643737 None:intergenic 50.0%
TGTTTCCATTGGCAGCATCG+AGG - chr8_2:64643748-64643767 Msa1262070:intron 50.0%
! GTTTCCATTGGCAGCATCGA+GGG - chr8_2:64643749-64643768 Msa1262070:intron 50.0%
ATCGAGGGAGATGTCCCTTT+GGG - chr8_2:64643764-64643783 Msa1262070:intron 50.0%
GCACCATGGCCAATTTGTGA+TGG - chr8_2:64644129-64644148 Msa1262070:intron 50.0%
! GATGCCATGGCGTTTTATGG+TGG - chr8_2:64644157-64644176 Msa1262070:intron 50.0%
!! TAGCAGATGGTGGATCACGA+CGG + chr8_2:64644353-64644372 None:intergenic 50.0%
AGCGTCACCATAGGTCACAA+TGG - chr8_2:64644495-64644514 Msa1262070:intron 50.0%
ACAGTGTTGTCCATGGAGGA+TGG + chr8_2:64644562-64644581 None:intergenic 50.0%
AGTGACAGTGTTGTCCATGG+AGG + chr8_2:64644566-64644585 None:intergenic 50.0%
CACAGTGACAGTGTTGTCCA+TGG + chr8_2:64644569-64644588 None:intergenic 50.0%
CTATAAAGCATGGGTACCCG+TGG - chr8_2:64644807-64644826 Msa1262070:intron 50.0%
ACCTACTTGCGTAACCACCA+CGG + chr8_2:64644827-64644846 None:intergenic 50.0%
CATAAGTCTTGCAGGGAAGG+TGG + chr8_2:64645449-64645468 None:intergenic 50.0%
CACCTTCCCTGCAAGACTTA+TGG - chr8_2:64645447-64645466 Msa1262070:CDS 50.0%
TGCCATAAGTCTTGCAGGGA+AGG + chr8_2:64645452-64645471 None:intergenic 50.0%
CCCTGCAAGACTTATGGCAA+AGG - chr8_2:64645453-64645472 Msa1262070:CDS 50.0%
TATGGCAAAGGAAGAGGAGC+AGG - chr8_2:64645465-64645484 Msa1262070:CDS 50.0%
GGCGGTGACATCTTACAGGT+TGG + chr8_2:64643118-64643137 None:intergenic 55.0%
TTGCGGCGGTGACATCTTAC+AGG + chr8_2:64643122-64643141 None:intergenic 55.0%
ACTGCCACCACCAAATTGGC+AGG - chr8_2:64643432-64643451 Msa1262070:intron 55.0%
! AGCTCCTGCCAATTTGGTGG+TGG + chr8_2:64643439-64643458 None:intergenic 55.0%
CTAGTTAGTCCTCGCAGCGA+CGG - chr8_2:64643457-64643476 Msa1262070:intron 55.0%
GCAGCAGCTGAGAAATTGGG+AGG - chr8_2:64643483-64643502 Msa1262070:intron 55.0%
!! AGCCAGCTAGGGGCACTTAT+TGG - chr8_2:64643713-64643732 Msa1262070:intron 55.0%
ATCTCCCTCGATGCTGCCAA+TGG + chr8_2:64643756-64643775 None:intergenic 55.0%
CATCGAGGGAGATGTCCCTT+TGG - chr8_2:64643763-64643782 Msa1262070:intron 55.0%
TCGAGGGAGATGTCCCTTTG+GGG - chr8_2:64643765-64643784 Msa1262070:intron 55.0%
!! TGTGATGGATGGCGATGCCA+TGG - chr8_2:64644144-64644163 Msa1262070:intron 55.0%
! GGCGATGCCATGGCGTTTTA+TGG - chr8_2:64644154-64644173 Msa1262070:intron 55.0%
! TGTGACCTATGGTGACGCTG+TGG + chr8_2:64644494-64644513 None:intergenic 55.0%
TAAAGCATGGGTACCCGTGG+TGG - chr8_2:64644810-64644829 Msa1262070:intron 55.0%
CCTACTTGCGTAACCACCAC+GGG + chr8_2:64644826-64644845 None:intergenic 55.0%
TGCAGCTGCAGTTGCAGTGT+CGG - chr8_2:64645146-64645165 Msa1262070:CDS 55.0%
AGTCTTGCAGGGAAGGTGGT+AGG + chr8_2:64645445-64645464 None:intergenic 55.0%
GGCAAGGTGGGAAGAACAAG+AGG + chr8_2:64645565-64645584 None:intergenic 55.0%
TCCACTGCACTTGGCAAGGT+GGG + chr8_2:64645577-64645596 None:intergenic 55.0%
GTTCTCCACTGCACTTGGCA+AGG + chr8_2:64645581-64645600 None:intergenic 55.0%
GGCTGTGGACAAGCCAGCTA+GGG - chr8_2:64643702-64643721 Msa1262070:intron 60.0%
GCTGTGGACAAGCCAGCTAG+GGG - chr8_2:64643703-64643722 Msa1262070:intron 60.0%
! AGGGAGATGTCCCTTTGGGG+AGG - chr8_2:64643768-64643787 Msa1262070:intron 60.0%
GTCTGCCACAGCGTCACCAT+AGG - chr8_2:64644486-64644505 Msa1262070:intron 60.0%
CCCGTGGTGGTTACGCAAGT+AGG - chr8_2:64644823-64644842 Msa1262070:intron 60.0%
TCCCACCTTGCCAAGTGCAG+TGG - chr8_2:64645573-64645592 Msa1262070:CDS 60.0%
CTCCACTGCACTTGGCAAGG+TGG + chr8_2:64645578-64645597 None:intergenic 60.0%
TGGGGATGGCACTAGGGCTG+TGG - chr8_2:64643687-64643706 Msa1262070:intron 65.0%
GGGCTGTGGACAAGCCAGCT+AGG - chr8_2:64643701-64643720 Msa1262070:intron 65.0%
TGCTGCTGTCCGTCGCTGCG+AGG + chr8_2:64643469-64643488 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 64643094 64645603 64643094 ID=Msa1262070;Name=Msa1262070
chr8_2 mRNA 64643094 64645603 64643094 ID=Msa1262070-mRNA-1;Parent=Msa1262070;Name=Msa1262070-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|0.66|1|1|3|0|336
chr8_2 exon 64644880 64645603 64644880 ID=Msa1262070-mRNA-1:exon:20908;Parent=Msa1262070-mRNA-1
chr8_2 exon 64644698 64644742 64644698 ID=Msa1262070-mRNA-1:exon:20907;Parent=Msa1262070-mRNA-1
chr8_2 exon 64643094 64643335 64643094 ID=Msa1262070-mRNA-1:exon:20906;Parent=Msa1262070-mRNA-1
chr8_2 CDS 64644880 64645603 64644880 ID=Msa1262070-mRNA-1:cds;Parent=Msa1262070-mRNA-1
chr8_2 CDS 64644698 64644742 64644698 ID=Msa1262070-mRNA-1:cds;Parent=Msa1262070-mRNA-1
chr8_2 CDS 64643094 64643335 64643094 ID=Msa1262070-mRNA-1:cds;Parent=Msa1262070-mRNA-1
Gene Sequence

>Msa1262070

ATGGTTCTTCCAGACAATATTCCAACCTGTAAGATGTCACCGCCGCAATTCACTACATCAAACAGCGATATCATTGAAGTGAGACCTCGATTTCCAATGAAAAATGAAAACTTTCAACAACATTCAACATTGAATCAACCCCATTCCTTCAAAAGGGCAAGAATTTCTGATAACAATCGTTCCAATGCTTTGATATGCCCTCCTCCAAAGATGGTTCTTCCTCCATCAAACAGAGCAGACCACGTTTTCTACAAGACTCGTATCTGTAATAAGTCCAGACTTGGTACTTGTAGAAACGGTAAAGATTGCAACTTTGCTCATGGAGTTGAAGAACTAAGACTGCCACCACCAAATTGGCAGGAGCTAGTTAGTCCTCGCAGCGACGGACAGCAGCAGCTGAGAAATTGGGAGGAAGATCAAAAAATGATTCACAAGATGAAGCTATGCAGGATGTATTCCAATGGTGAGAAATGTTTCTTTGGTAGTAAGTGTTATTTTCTTCATGAGGATCCTGCTAAGTTTAGGGATCATTCTTGGAAGTCAAGAGAGTGTTCTTCTATAAGCATTGGGGCTATTGGTTCTTCAAAGTCATTTGGGGATGGCACTAGGGCTGTGGACAAGCCAGCTAGGGGCACTTATTGGAAGACTAAGACGTGTTTCCATTGGCAGCATCGAGGGAGATGTCCCTTTGGGGAGGACTGTCAATTTGCTCATGGAGAAGCAGAAGATCATTTTATGTTATATGATGTTCAAATTATAGTGGTTGAATTTGCAATAGCAAATTCAACAAAAGCTATCATTCCAACTTCACGAAAAGCCTTAACAGGTTCTGCCCATGATGCATATGCACCTACCACCTTCCCTGCAAGACTTATGGCAAAGGAAGAGGAGCAGGCTAAGAAGATGCATTTACTTTGGTTGAAATTAAACAAGATCAATCGTGTTTATGGTGATTGGATTGATGATTCACCTCTTGTTCTTCCCACCTTGCCAAGTGCAGTGGAGAACTGA
Protein sequence

>Msa1262070

MVLPDNIPTCKMSPPQFTTSNSDIIEVRPRFPMKNENFQQHSTLNQPHSFKRARISDNNRSNALICPPPKMVLPPSNRADHVFYKTRICNKSRLGTCRNGKDCNFAHGVEELRLPPPNWQELVSPRSDGQQQLRNWEEDQKMIHKMKLCRMYSNGEKCFFGSKCYFLHEDPAKFRDHSWKSRECSSISIGAIGSSKSFGDGTRAVDKPARGTYWKTKTCFHWQHRGRCPFGEDCQFAHGEAEDHFMLYDVQIIVVEFAIANSTKAIIPTSRKALTGSAHDAYAPTTFPARLMAKEEEQAKKMHLLWLKLNKINRVYGDWIDDSPLVLPTLPSAVEN*