Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1262520 | AES91783.1 | 91.160 | 181 | 16 | 0 | 1 | 181 | 1 | 181 | 6.23e-112 | 326 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1262520 | G7JJ33 | 91.160 | 181 | 16 | 0 | 1 | 181 | 1 | 181 | 2.98e-112 | 326 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1262520 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1262520 | MtrunA17_Chr4g0066461 | 45.045 | 111 | 42 | 3 | 77 | 181 | 1 | 98 | 2.50e-20 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGCTCTGTTACGGTTAC+TGG | 0.180827 | 8_2:-65323765 | Msa1262520:CDS |
| TCAAGCAATGTGGCTTTCTT+TGG | 0.199938 | 8_2:-65323741 | Msa1262520:CDS |
| TGTTGGTTCCGTGTCGTAAT+AGG | 0.277898 | 8_2:-65323794 | Msa1262520:CDS |
| GAGAGAGATTATGAGTTTGT+AGG | 0.295578 | 8_2:-65323556 | None:intergenic |
| GCTAATGTTTCTGTTAATAA+TGG | 0.298819 | 8_2:-65323639 | Msa1262520:CDS |
| CAAGCAATGTGGCTTTCTTT+GGG | 0.307847 | 8_2:-65323740 | Msa1262520:CDS |
| ATCACTGCTTTGGTTCAAAA+TGG | 0.315294 | 8_2:-65323600 | Msa1262520:CDS |
| TAACAAATCAGTTTCTGAAA+TGG | 0.363545 | 8_2:-65324211 | Msa1262520:CDS |
| TAATAATGGTAATTTGATCT+TGG | 0.376074 | 8_2:-65323625 | Msa1262520:CDS |
| GATCTTGGCGATCACTGCTT+TGG | 0.383378 | 8_2:-65323610 | Msa1262520:CDS |
| AACAAATCAGTTTCTGAAAT+GGG | 0.410347 | 8_2:-65324210 | Msa1262520:CDS |
| TTACATTGGGGTAGAGAGTT+GGG | 0.421719 | 8_2:+65324082 | None:intergenic |
| GAAAATGTTGGAGGAGATGA+AGG | 0.445802 | 8_2:-65323856 | Msa1262520:CDS |
| GAATCTCAATTGCTCTGTTA+CGG | 0.448731 | 8_2:-65323772 | Msa1262520:CDS |
| GTTACATTGGGGTAGAGAGT+TGG | 0.468914 | 8_2:+65324081 | None:intergenic |
| ATCAAAGAAACCTGTTGAAG+GGG | 0.470795 | 8_2:-65323826 | Msa1262520:CDS |
| TGAAGAAAAGAAGAAAATGT+TGG | 0.489430 | 8_2:-65323868 | Msa1262520:CDS |
| TTTCTTTGGGATTGAATCTG+TGG | 0.501659 | 8_2:-65323727 | Msa1262520:CDS |
| GAGTTGGGACAGTGGTAGTG+AGG | 0.504434 | 8_2:+65324097 | None:intergenic |
| ATTGAATCTGTGGCTAAACC+TGG | 0.515198 | 8_2:-65323717 | Msa1262520:CDS |
| TTCAACGTTTAGAACCTCTT+CGG | 0.524218 | 8_2:+65324158 | None:intergenic |
| AAATCAAAGAAACCTGTTGA+AGG | 0.526426 | 8_2:-65323828 | Msa1262520:CDS |
| AGAAAAGAAGAAAATGTTGG+AGG | 0.526690 | 8_2:-65323865 | Msa1262520:CDS |
| AATCAAAGAAACCTGTTGAA+GGG | 0.548949 | 8_2:-65323827 | Msa1262520:CDS |
| TAAACGTTGAACGTGAAAGA+AGG | 0.567677 | 8_2:-65324146 | Msa1262520:CDS |
| TCAAAGAAACCTGTTGAAGG+GGG | 0.622647 | 8_2:-65323825 | Msa1262520:CDS |
| CACTACTACTAAAACCGAAG+AGG | 0.646167 | 8_2:-65324172 | Msa1262520:CDS |
| AAAATCACAGTAATCAAACC+AGG | 0.647796 | 8_2:+65323699 | None:intergenic |
| GGTTACTGGTTCAAGCAATG+TGG | 0.661828 | 8_2:-65323751 | Msa1262520:CDS |
| GGGGTAGAGAGTTGGGACAG+TGG | 0.661869 | 8_2:+65324089 | None:intergenic |
| TACACAGGAAGTGATTGTGA+AGG | 0.673700 | 8_2:-65323916 | Msa1262520:CDS |
| TTGAGATTCCTATTACGACA+CGG | 0.693765 | 8_2:+65323786 | None:intergenic |
| GAAATGGGTAACAGAAAACG+TGG | 0.703774 | 8_2:-65324195 | Msa1262520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAATAAAAGAGAAAA+TGG | + | chr8_2:65323741-65323760 | None:intergenic | 15.0% |
| !! | ATAACAAATCAAACTATAAT+GGG | + | chr8_2:65323821-65323840 | None:intergenic | 15.0% |
| !! | AAAATAAAAGAGAAAATGGA+AGG | + | chr8_2:65323737-65323756 | None:intergenic | 20.0% |
| !! | GATAACAAATCAAACTATAA+TGG | + | chr8_2:65323822-65323841 | None:intergenic | 20.0% |
| !!! | TTTGATTTGTTATCTGAAAT+GGG | - | chr8_2:65323828-65323847 | Msa1262520:CDS | 20.0% |
| !! | TAATAATGGTAATTTGATCT+TGG | - | chr8_2:65324157-65324176 | Msa1262520:CDS | 20.0% |
| !! | TAACAAATCAGTTTCTGAAA+TGG | - | chr8_2:65323571-65323590 | Msa1262520:CDS | 25.0% |
| !!! | AACAAATCAGTTTCTGAAAT+GGG | - | chr8_2:65323572-65323591 | Msa1262520:CDS | 25.0% |
| ! | TTCATTCTTCATACTTCATT+TGG | - | chr8_2:65323755-65323774 | Msa1262520:CDS | 25.0% |
| ! | TCATTCTTCATACTTCATTT+GGG | - | chr8_2:65323756-65323775 | Msa1262520:CDS | 25.0% |
| !!! | GTTTGATTTGTTATCTGAAA+TGG | - | chr8_2:65323827-65323846 | Msa1262520:CDS | 25.0% |
| !!! | TGAAATGGGTTTTTGTTTTT+AGG | - | chr8_2:65323842-65323861 | Msa1262520:CDS | 25.0% |
| ! | TGAAGAAAAGAAGAAAATGT+TGG | - | chr8_2:65323914-65323933 | Msa1262520:CDS | 25.0% |
| !! | TTTGATTACTGTGATTTTGA+AGG | - | chr8_2:65324088-65324107 | Msa1262520:CDS | 25.0% |
| !! | GCTAATGTTTCTGTTAATAA+TGG | - | chr8_2:65324143-65324162 | Msa1262520:CDS | 25.0% |
| GAATGATGTTAGTTACATTG+GGG | + | chr8_2:65323715-65323734 | None:intergenic | 30.0% | |
| GGAATGATGTTAGTTACATT+GGG | + | chr8_2:65323716-65323735 | None:intergenic | 30.0% | |
| AGGAATGATGTTAGTTACAT+TGG | + | chr8_2:65323717-65323736 | None:intergenic | 30.0% | |
| !!! | TTTTTGTTTTTAGGCTACAC+AGG | - | chr8_2:65323851-65323870 | Msa1262520:CDS | 30.0% |
| AGAAAAGAAGAAAATGTTGG+AGG | - | chr8_2:65323917-65323936 | Msa1262520:CDS | 30.0% | |
| AAATCAAAGAAACCTGTTGA+AGG | - | chr8_2:65323954-65323973 | Msa1262520:intron | 30.0% | |
| AATCAAAGAAACCTGTTGAA+GGG | - | chr8_2:65323955-65323974 | Msa1262520:intron | 30.0% | |
| AAAATCACAGTAATCAAACC+AGG | + | chr8_2:65324086-65324105 | None:intergenic | 30.0% | |
| ! | TTCAACGTTTAGAACCTCTT+CGG | + | chr8_2:65323627-65323646 | None:intergenic | 35.0% |
| TAAACGTTGAACGTGAAAGA+AGG | - | chr8_2:65323636-65323655 | Msa1262520:CDS | 35.0% | |
| ATCAAAGAAACCTGTTGAAG+GGG | - | chr8_2:65323956-65323975 | Msa1262520:intron | 35.0% | |
| AAATTAAAACCCCCTTCAAC+AGG | + | chr8_2:65323969-65323988 | None:intergenic | 35.0% | |
| !!! | TGAAGGGGGTTTTAATTTGT+TGG | - | chr8_2:65323971-65323990 | Msa1262520:intron | 35.0% |
| TTGAGATTCCTATTACGACA+CGG | + | chr8_2:65323999-65324018 | None:intergenic | 35.0% | |
| GAATCTCAATTGCTCTGTTA+CGG | - | chr8_2:65324010-65324029 | Msa1262520:intron | 35.0% | |
| !! | TTTCTTTGGGATTGAATCTG+TGG | - | chr8_2:65324055-65324074 | Msa1262520:intron | 35.0% |
| !! | GTTTTTGAAGAATGAAGCTG+AGG | - | chr8_2:65324112-65324131 | Msa1262520:CDS | 35.0% |
| ! | ATCACTGCTTTGGTTCAAAA+TGG | - | chr8_2:65324182-65324201 | Msa1262520:CDS | 35.0% |
| GAAATGGGTAACAGAAAACG+TGG | - | chr8_2:65323587-65323606 | Msa1262520:CDS | 40.0% | |
| CACTACTACTAAAACCGAAG+AGG | - | chr8_2:65323610-65323629 | Msa1262520:CDS | 40.0% | |
| TTACATTGGGGTAGAGAGTT+GGG | + | chr8_2:65323703-65323722 | None:intergenic | 40.0% | |
| TACACAGGAAGTGATTGTGA+AGG | - | chr8_2:65323866-65323885 | Msa1262520:CDS | 40.0% | |
| GAAAATGTTGGAGGAGATGA+AGG | - | chr8_2:65323926-65323945 | Msa1262520:intron | 40.0% | |
| TCAAAGAAACCTGTTGAAGG+GGG | - | chr8_2:65323957-65323976 | Msa1262520:intron | 40.0% | |
| AATTGCTCTGTTACGGTTAC+TGG | - | chr8_2:65324017-65324036 | Msa1262520:intron | 40.0% | |
| TCAAGCAATGTGGCTTTCTT+TGG | - | chr8_2:65324041-65324060 | Msa1262520:intron | 40.0% | |
| CAAGCAATGTGGCTTTCTTT+GGG | - | chr8_2:65324042-65324061 | Msa1262520:intron | 40.0% | |
| ATTGAATCTGTGGCTAAACC+TGG | - | chr8_2:65324065-65324084 | Msa1262520:intron | 40.0% | |
| GTTACATTGGGGTAGAGAGT+TGG | + | chr8_2:65323704-65323723 | None:intergenic | 45.0% | |
| !! | TGTTGGTTCCGTGTCGTAAT+AGG | - | chr8_2:65323988-65324007 | Msa1262520:intron | 45.0% |
| !! | GGTTACTGGTTCAAGCAATG+TGG | - | chr8_2:65324031-65324050 | Msa1262520:intron | 45.0% |
| GATCTTGGCGATCACTGCTT+TGG | - | chr8_2:65324172-65324191 | Msa1262520:CDS | 50.0% | |
| ! | GAGTTGGGACAGTGGTAGTG+AGG | + | chr8_2:65323688-65323707 | None:intergenic | 55.0% |
| GGGGTAGAGAGTTGGGACAG+TGG | + | chr8_2:65323696-65323715 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 65323557 | 65324247 | 65323557 | ID=Msa1262520;Name=Msa1262520 |
| chr8_2 | mRNA | 65323557 | 65324247 | 65323557 | ID=Msa1262520-mRNA-1;Parent=Msa1262520;Name=Msa1262520-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|181 |
| chr8_2 | exon | 65324086 | 65324247 | 65324086 | ID=Msa1262520-mRNA-1:exon:21170;Parent=Msa1262520-mRNA-1 |
| chr8_2 | exon | 65323557 | 65323940 | 65323557 | ID=Msa1262520-mRNA-1:exon:21169;Parent=Msa1262520-mRNA-1 |
| chr8_2 | CDS | 65324086 | 65324247 | 65324086 | ID=Msa1262520-mRNA-1:cds;Parent=Msa1262520-mRNA-1 |
| chr8_2 | CDS | 65323557 | 65323940 | 65323557 | ID=Msa1262520-mRNA-1:cds;Parent=Msa1262520-mRNA-1 |
| Gene Sequence |
| Protein sequence |
