AlfalfaGEDB | Msa1265360

M. sativa cultivar zhongmu-4 / Msa1265360

KEGG: K19199
GO: -
PFAM: PF09273.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1265360	AES92327.2	97.441	547	14	0	1	547	93	639	0.0	1108

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1265360	G7JVG8	97.441	547	14	0	1	547	93	639	0.0	1108

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TFs/TRs:

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Gene ID	Type	Classification
Msa1265360	TR	SET

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1265360	MtrunA17_Chr4g0072481	97.441	547	14	0	1	547	1	547	0.0	1102
Msa1265360	MtrunA17_Chr3g0120751	21.951	410	278	12	142	518	63	463	4.81e-18	87.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1265360	AT2G18850.1	52.060	534	250	4	5	537	10	538	0.0	558
Msa1265360	AT2G18850.2	51.685	534	245	5	5	537	10	531	0.0	550
Msa1265360	AT3G07670.1	23.840	474	310	18	82	520	15	472	7.90e-21	96.3
Msa1265360	AT3G07670.2	24.528	318	200	11	234	520	26	334	1.87e-15	78.6

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Find 133 sgRNAs with CRISPR-Local

Find 711 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCCATGGTGTGAAGAC+AGG	0.508669	8_2:+69630266	Msa1265360:CDS
AAAGCACTAAATCTACCGCG+TGG	0.790865	8_2:-69638826	None:intergenic
AAAGTCTTTGTTTGCATCAC+AGG	0.436089	8_2:-69637810	None:intergenic
AAATGTAATAAAATGAGAGC+TGG	0.529710	8_2:-69637443	None:intergenic
AAGAATCATACAGTTAAACG+AGG	0.733021	8_2:+69629727	Msa1265360:CDS
AAGAATGCTGCTTAAGCTAT+GGG	0.536764	8_2:+69637413	Msa1265360:CDS
AAGATGATCCAATAATGGAG+AGG	0.665042	8_2:-69637772	None:intergenic
AAGCTCCTCAGAAATGATAA+TGG	0.442652	8_2:-69630507	None:intergenic
AATCACAGCATATTCTATTA+TGG	0.363717	8_2:+69637744	Msa1265360:CDS
AATTTCAATGAATTTGTTGA+AGG	0.427682	8_2:-69637361	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTATATCATGTACAAACCCT+AGG	-	chr8_2:69628766-69628785	None:intergenic	30.0%
ATCAGTAATATCAAGGTATG+AGG	-	chr8_2:69628977-69628996	None:intergenic	30.0%
TCCTTTCTTTGATGAAAAGA+AGG	+	chr8_2:69629182-69629201	Msa1265360:CDS	30.0%
AATGATGTAAGTCTTCCATA+TGG	+	chr8_2:69629322-69629341	Msa1265360:intron	30.0%
TATATTGCCTTGAATAACGT+TGG	-	chr8_2:69629455-69629474	None:intergenic	30.0%
TTATACTGTAACGACTAATC+TGG	+	chr8_2:69629580-69629599	Msa1265360:intron	30.0%
TCAGAAATTACTACGATGTA+AGG	+	chr8_2:69629616-69629635	Msa1265360:intron	30.0%
CAGAAATTACTACGATGTAA+GGG	+	chr8_2:69629617-69629636	Msa1265360:intron	30.0%
AGAAATTACTACGATGTAAG+GGG	+	chr8_2:69629618-69629637	Msa1265360:intron	30.0%
AAGAATCATACAGTTAAACG+AGG	+	chr8_2:69629727-69629746	Msa1265360:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	69628412	69639123	69628412	ID=Msa1265360;Name=Msa1265360
chr8_2	mRNA	69628412	69639123	69628412	ID=Msa1265360-mRNA-1;Parent=Msa1265360;Name=Msa1265360-mRNA-1;_AED=0.14;_eAED=0.14;_QI=114\|0.90\|1\|1\|0.90\|0.83\|12\|303\|547
chr8_2	exon	69628412	69628523	69628412	ID=Msa1265360-mRNA-1:exon:23029;Parent=Msa1265360-mRNA-1
chr8_2	exon	69629130	69629203	69629130	ID=Msa1265360-mRNA-1:exon:23030;Parent=Msa1265360-mRNA-1
chr8_2	exon	69629620	69629748	69629620	ID=Msa1265360-mRNA-1:exon:23031;Parent=Msa1265360-mRNA-1
chr8_2	exon	69630008	69630302	69630008	ID=Msa1265360-mRNA-1:exon:23032;Parent=Msa1265360-mRNA-1
chr8_2	exon	69630429	69630547	69630429	ID=Msa1265360-mRNA-1:exon:23033;Parent=Msa1265360-mRNA-1
chr8_2	exon	69631126	69631258	69631126	ID=Msa1265360-mRNA-1:exon:23034;Parent=Msa1265360-mRNA-1
chr8_2	exon	69633695	69633771	69633695	ID=Msa1265360-mRNA-1:exon:23035;Parent=Msa1265360-mRNA-1
chr8_2	exon	69636629	69636826	69636629	ID=Msa1265360-mRNA-1:exon:23036;Parent=Msa1265360-mRNA-1
chr8_2	exon	69637325	69637514	69637325	ID=Msa1265360-mRNA-1:exon:23037;Parent=Msa1265360-mRNA-1
chr8_2	exon	69637655	69637833	69637655	ID=Msa1265360-mRNA-1:exon:23038;Parent=Msa1265360-mRNA-1
chr8_2	exon	69638170	69638270	69638170	ID=Msa1265360-mRNA-1:exon:23039;Parent=Msa1265360-mRNA-1
chr8_2	exon	69638670	69639123	69638670	ID=Msa1265360-mRNA-1:exon:23040;Parent=Msa1265360-mRNA-1
chr8_2	five_prime_UTR	69628412	69628523	69628412	ID=Msa1265360-mRNA-1:five_prime_utr;Parent=Msa1265360-mRNA-1
chr8_2	five_prime_UTR	69629130	69629131	69629130	ID=Msa1265360-mRNA-1:five_prime_utr;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69629132	69629203	69629132	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69629620	69629748	69629620	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69630008	69630302	69630008	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69630429	69630547	69630429	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69631126	69631258	69631126	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69633695	69633771	69633695	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69636629	69636826	69636629	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69637325	69637514	69637325	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69637655	69637833	69637655	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69638170	69638270	69638170	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	CDS	69638670	69638820	69638670	ID=Msa1265360-mRNA-1:cds;Parent=Msa1265360-mRNA-1
chr8_2	three_prime_UTR	69638821	69639123	69638821	ID=Msa1265360-mRNA-1:three_prime_utr;Parent=Msa1265360-mRNA-1

Gene Sequence

>Msa1265360

ATGGATAGTGACGAGGAGTGCTCAATTGTTCTTGAGTTATCTGAAAATGATCCTTTCTTTGATGAAAAGAAGAAATTACTACGATGTAAGGGGTTTGGTCCCAAGGAACGCATACATCTCAAGCACTCCTCAGGACCTTCTTGGATGAATGATATCGTAAAGATCTTACTTCAGATTGCAAGAATCATACAGTTAAACGAGCTGGAACTCTATTTTGCTGAAGACGATGCCTCGGTGGAGTTTTACAGCCCCAGGAATGAGTTGGAGGCTCTCAATTCAATTGTTTCGCTCACAGACAAATCACTTTCTAGTTGTCCACATTTCCATACAAACGTCCTGCAAGGGTTACAACAAAAGATTCTTGATTTAATTTCTGATTTTGGGGACCAAAACAATATGAAGGGAGTACTTGAAAAGTACCACAGTTGTGAGAGAGAAGAACACCTGATTGAGTGGGGTAAAAGCCATGGTGTGAAGACAGGATTAAAGATAGCTTATGTTGAAGGAGCAGGTCGGGGAATGATAGCAGGGAAAGATCTAAAAGTTGGAGATACTGCTATAGAGATTCCCGTGTCCATTATCATTTCTGAGGAGCTTGTGCTTGAAACTGATATGTATCGTCTCTTGAAAGAGGTTGATGGCATATCATCTGAGACAATATTGCTGTTATGGAGTATGAAAGAGAAGTACAACTGTGATTCAAAATTCAAAATTTACTTTGACACACTTCCAGAAAAATTTAATACAGGATTGAGTTTTGGGATTGAAGCAATTACAATGCTAGATGGAACCTTGCTGTTTGAAGAGATAATGCAATCAAGACAGCACTTGCATGCTCAGTATGATGAGTTGGTTCCTGCTTTGTGCAATGGTTTTCCTGATATATTTCCTCCAGAGATTTACACTTGGGAGAATTTTTTATGGGCTTGTGAACTTTGGTATTCCAATAGCATGAAGATAATGTATTCAGATGGAAAACTGAGGACATGTTTGATACCTCTTGCTGGCTTTCTTAATCATTCACTGTGCCCACATATTACACACTATGGCAAAGTAGATCCTTCAACAAATTCATTGAAATTTTGTCTATCAAGACCATGCCGATCTGGAGAAGAATGCTGCTTAAGCTATGGGAACTTCTCCAGCTCTCATTTTATTACATTTTATGGTTTCTTACCTCAAGGAGACAACCCGTATGATGTCATTCCTTTAGACATCGATAGTTCTGATGTTGATTCTATTGAGGATAAACCTGTGTCCAAATGGACGACTCACATGGTGCGTGGTACCTGGCTCTCCAACAATCACAGCATATTCTATTATGGTCTTCCCTCTCCATTATTGGATCATCTTCGGAGATCTCGAAGTCCTGTGATGCAAACAAAGACTTTCTTGCAAGGAAACTTGGAAAATGAGCTAGAAGTTCTTGGAGATCTCAAAGACATCTTTGACGACATGATGGACAGTATGGGTGAAGTCGATCTTGATAACAGAGAAAGCTACACCTGGGATGAAAAATTGGCTGTGGATTGTAAAAATTTACAAAGACGAATAGCCCATTCTGTTTCAACCTCTTGCTGTACTGGGATCAACATGTTGAAGAATGAACTGCACAAGTGTTTGGCTGAGGACATTGTAGGTTAA

Protein sequence

>Msa1265360

MDSDEECSIVLELSENDPFFDEKKKLLRCKGFGPKERIHLKHSSGPSWMNDIVKILLQIARIIQLNELELYFAEDDASVEFYSPRNELEALNSIVSLTDKSLSSCPHFHTNVLQGLQQKILDLISDFGDQNNMKGVLEKYHSCEREEHLIEWGKSHGVKTGLKIAYVEGAGRGMIAGKDLKVGDTAIEIPVSIIISEELVLETDMYRLLKEVDGISSETILLLWSMKEKYNCDSKFKIYFDTLPEKFNTGLSFGIEAITMLDGTLLFEEIMQSRQHLHAQYDELVPALCNGFPDIFPPEIYTWENFLWACELWYSNSMKIMYSDGKLRTCLIPLAGFLNHSLCPHITHYGKVDPSTNSLKFCLSRPCRSGEECCLSYGNFSSSHFITFYGFLPQGDNPYDVIPLDIDSSDVDSIEDKPVSKWTTHMVRGTWLSNNHSIFYYGLPSPLLDHLRRSRSPVMQTKTFLQGNLENELEVLGDLKDIFDDMMDSMGEVDLDNRESYTWDEKLAVDCKNLQRRIAHSVSTSCCTGINMLKNELHKCLAEDIVG*