AlfalfaGEDB | Msa1265380

M. sativa cultivar zhongmu-4 / Msa1265380

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1265380	AES92320.1	55.639	133	15	1	233	365	151	239	8.90e-32	130
Msa1265380	AES92320.1	59.561	319	47	5	1	319	1	237	3.52e-110	332

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1265380	sp\|O80883\|MB101_ARATH	50.000	120	59	1	9	128	16	134	2.46e-38	146

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa1265380	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1265380	MtrunA17_Chr5g0441141	70.492	244	47	7	12	251	13	235	1.75e-110	323
Msa1265380	MtrunA17_Chr5g0440761	71.429	238	47	6	12	247	13	231	1.23e-109	321
Msa1265380	MtrunA17_Chr5g0440781	80.110	181	28	2	12	192	13	185	4.14e-101	296
Msa1265380	MtrunA17_Chr5g0441131	64.706	187	46	3	2	188	4	170	1.43e-76	234
Msa1265380	MtrunA17_Chr4g0072451	60.759	158	16	3	1	158	1	112	4.20e-51	167
Msa1265380	MtrunA17_Chr5g0425591	82.979	94	12	1	2	95	4	93	6.84e-51	165
Msa1265380	MtrunA17_Chr1g0154031	43.787	169	91	3	11	177	76	242	1.44e-39	145
Msa1265380	MtrunA17_Chr1g0192411	44.500	200	95	8	2	191	4	197	1.76e-39	144
Msa1265380	MtrunA17_Chr3g0080861	41.104	163	93	3	11	171	37	198	2.91e-39	146
Msa1265380	MtrunA17_Chr4g0048511	49.630	135	61	3	6	134	8	141	1.29e-38	141

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1265380	AT2G26960.1	47.500	160	71	4	9	158	18	174	2.23e-43	155
Msa1265380	AT2G32460.2	50.000	120	59	1	9	128	4	122	2.40e-39	146
Msa1265380	AT2G32460.1	50.000	120	59	1	9	128	16	134	2.50e-39	146
Msa1265380	AT4G21440.1	55.263	114	49	2	10	122	11	123	1.99e-38	140
Msa1265380	AT5G55020.1	53.509	114	52	1	11	124	26	138	2.54e-38	144
Msa1265380	AT4G26930.1	48.760	121	61	1	11	131	19	138	1.03e-37	140
Msa1265380	AT5G10280.1	37.900	219	107	6	3	198	4	216	1.68e-37	138
Msa1265380	AT5G06100.1	50.000	120	59	1	10	129	31	149	2.35e-37	140
Msa1265380	AT5G56110.1	51.282	117	55	2	11	126	12	127	3.50e-37	137
Msa1265380	AT3G11440.2	49.587	121	60	1	11	131	41	160	4.31e-37	139

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Find 79 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAACTTAAAAGGCG+TGG	0.582597	8_2:+69661089	Msa1265380:CDS
AAAATTCTTGATGTCATTAT+CGG	0.314551	8_2:-69661054	None:intergenic
AAACATGCTCTACAATAATG+TGG	0.540325	8_2:+69661295	Msa1265380:CDS
AAAGAAGCCAATTTCTTTGG+AGG	0.456698	8_2:+69661874	Msa1265380:CDS
AAAGGACCCTTCTTCACATC+TGG	0.443578	8_2:-69660479	None:intergenic
AACATGCTCTACAATAATGT+GGG	0.582958	8_2:+69661296	Msa1265380:CDS
AACGAGGTATCAAGAAATGC+GGG	0.517508	8_2:+69660404	Msa1265380:CDS
AACGATGTTGCAGCAAGCCA+TGG	0.571591	8_2:+69661764	Msa1265380:CDS
AATGCGGGGCTTATACGTTG+CGG	0.702899	8_2:+69660419	Msa1265380:CDS
ACAAATTTGGTTGATGAAAA+AGG	0.207164	8_2:+69661851	Msa1265380:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCATCAATGGATACTAACAA+AGG	+	chr8_2:69660302-69660321	Msa1265380:CDS	30.0%
CATCAATGGATACTAACAAA+GGG	+	chr8_2:69660303-69660322	Msa1265380:CDS	30.0%
ACTAACAAAGGGTTGAAAAA+AGG	+	chr8_2:69660314-69660333	Msa1265380:CDS	30.0%
AAGGGTTGAAAAAAGGAAAA+TGG	+	chr8_2:69660321-69660340	Msa1265380:CDS	30.0%
AAAGCATATGTGGAAAAACA+TGG	+	chr8_2:69660368-69660387	Msa1265380:CDS	30.0%
AAAAACATGGAACCAGAAAT+TGG	+	chr8_2:69660381-69660400	Msa1265380:CDS	30.0%
GTTAAAGAACTGAAAGACTT+TGG	-	chr8_2:69660522-69660541	None:intergenic	30.0%
AATGTGTGATCTAACGAATA+TGG	-	chr8_2:69660657-69660676	None:intergenic	30.0%
TACTAAACAACTTATAGGCA+TGG	-	chr8_2:69660911-69660930	None:intergenic	30.0%
TGATAAGTTGCATGTGAATA+AGG	+	chr8_2:69660936-69660955	Msa1265380:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	69660296	69661958	69660296	ID=Msa1265380;Name=Msa1265380
chr8_2	mRNA	69660296	69661958	69660296	ID=Msa1265380-mRNA-1;Parent=Msa1265380;Name=Msa1265380-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|0\|0\|1\|1\|1\|3\|0\|365
chr8_2	exon	69660296	69660580	69660296	ID=Msa1265380-mRNA-1:exon:23026;Parent=Msa1265380-mRNA-1
chr8_2	exon	69661041	69661360	69661041	ID=Msa1265380-mRNA-1:exon:23027;Parent=Msa1265380-mRNA-1
chr8_2	exon	69661466	69661958	69661466	ID=Msa1265380-mRNA-1:exon:23028;Parent=Msa1265380-mRNA-1
chr8_2	CDS	69660296	69660580	69660296	ID=Msa1265380-mRNA-1:cds;Parent=Msa1265380-mRNA-1
chr8_2	CDS	69661041	69661360	69661041	ID=Msa1265380-mRNA-1:cds;Parent=Msa1265380-mRNA-1
chr8_2	CDS	69661466	69661958	69661466	ID=Msa1265380-mRNA-1:cds;Parent=Msa1265380-mRNA-1

Gene Sequence

>Msa1265380

ATGTCTTCATCAATGGATACTAACAAAGGGTTGAAAAAAGGAAAATGGTCAAAAGAAGAGGATGAAATGTTAAAAGCATATGTGGAAAAACATGGAACCAGAAATTGGAACGAGGTATCAAGAAATGCGGGGCTTATACGTTGCGGTAAAAGTTGTCGTCTAAGATGGTATAATCACCTTCAACCAGATGTGAAGAAGGGTCCTTTTAGTGAAGAAGAAAAAACCAAAGTCTTTCAGTTCTTTAACATGTTTGGTGAATTCAAATGGTCTAAGCTGGCTCACGAGTTGCCTGGAAGATCCGATAATGACATCAAGAATTTTTGGAATGCAAGAAAAAGAAAACTTAAAAGGCGTGGCTTGTCACCCTTCCCTGACAAGATGGAACTGAATGATGAGTTGAATAAAAATGGTTCAAATAGCCAACAAGTTAAGGATTCACAAGATGATGAATTTTATATACCACAAGTGAAATTTGGAAAATATTCATCAATTTATGATAAGATAAGTGAAAACAACTTTGAAAAACTTTTAGATGTCCCAAACATGCTCTACAATAATGTGGGAAGTACTTCTACAAGCAATCATACTACTTCAATGATTTATAGGGGTAGCATTGGAACTTCTTGTATACCTATGGCTATGGTATCAGATCTGCCTCCACCCTCAGAATGTTCATACCTTCCACTACTTCCTTCACGTATGATGCTTGGTGAGTATCCTCAAATTCCACCACCTATGGAGACTCGATATAAGATGTCAGGATCAAGTTCTGCGTATAAAAGTGTCTCATTGGAGTCTATGTTATATAGACAAGAGAATTGGGAAGGCTTCGATGCTAGTCACGTGCATTCCTCTGAGATAAATTTGGTTGATGAAAAAGGGAAGAAGTCAATTGCTTTGGAGAACGATGTTGCAGCAAGCCATGGTGACTTTATGGATCTGGATCTTCGGTTAGACAATAACGATGCTAGGCATGTGCATTCGTCTGAGACAAATTTGGTTGATGAAAAAGGAAAGAAGCCAATTTCTTTGGAGGACAATGTTGAAGCAAACCTTGGTGACTTCATGGATCCGTGGCTTTTGATTGACTTCAGTTGA

Protein sequence

>Msa1265380

MSSSMDTNKGLKKGKWSKEEDEMLKAYVEKHGTRNWNEVSRNAGLIRCGKSCRLRWYNHLQPDVKKGPFSEEEKTKVFQFFNMFGEFKWSKLAHELPGRSDNDIKNFWNARKRKLKRRGLSPFPDKMELNDELNKNGSNSQQVKDSQDDEFYIPQVKFGKYSSIYDKISENNFEKLLDVPNMLYNNVGSTSTSNHTTSMIYRGSIGTSCIPMAMVSDLPPPSECSYLPLLPSRMMLGEYPQIPPPMETRYKMSGSSSAYKSVSLESMLYRQENWEGFDASHVHSSEINLVDEKGKKSIALENDVAASHGDFMDLDLRLDNNDARHVHSSETNLVDEKGKKPISLEDNVEANLGDFMDPWLLIDFS*