Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1275060 | sp|P0DH62|Y4407_SELML | 45.517 | 580 | 205 | 11 | 104 | 677 | 5 | 479 | 6.82e-149 | 449 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1275060 | A0A072TXS4 | 98.675 | 755 | 10 | 0 | 1 | 755 | 1 | 755 | 0.0 | 1548 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1275060 | MtrunA17_Chr8g0341881 | 98.675 | 755 | 10 | 0 | 1 | 755 | 1 | 755 | 0.0 | 1548 |
| Msa1275060 | MtrunA17_Chr2g0330471 | 80.029 | 691 | 124 | 8 | 1 | 683 | 1 | 685 | 0.0 | 1099 |
| Msa1275060 | MtrunA17_Chr8g0389251 | 64.000 | 675 | 233 | 7 | 12 | 681 | 11 | 680 | 0.0 | 857 |
| Msa1275060 | MtrunA17_Chr1g0154291 | 45.777 | 592 | 268 | 15 | 143 | 700 | 1 | 573 | 2.12e-147 | 444 |
| Msa1275060 | MtrunA17_Chr3g0125631 | 52.427 | 309 | 145 | 2 | 371 | 678 | 39 | 346 | 1.40e-111 | 343 |
| Msa1275060 | MtrunA17_Chr3g0080901 | 53.311 | 302 | 132 | 5 | 374 | 675 | 98 | 390 | 3.18e-99 | 315 |
| Msa1275060 | MtrunA17_Chr7g0221191 | 49.057 | 318 | 152 | 4 | 392 | 700 | 382 | 698 | 1.50e-97 | 318 |
| Msa1275060 | MtrunA17_Chr3g0144481 | 48.276 | 319 | 153 | 5 | 369 | 682 | 59 | 370 | 2.09e-96 | 306 |
| Msa1275060 | MtrunA17_Chr5g0413511 | 48.580 | 317 | 151 | 4 | 377 | 682 | 25 | 340 | 1.41e-95 | 302 |
| Msa1275060 | MtrunA17_Chr6g0485761 | 48.624 | 327 | 156 | 4 | 370 | 691 | 266 | 585 | 2.29e-94 | 308 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1275060 | AT3G13690.2 | 73.553 | 760 | 173 | 15 | 1 | 748 | 1 | 744 | 0.0 | 1090 |
| Msa1275060 | AT3G13690.1 | 73.553 | 760 | 173 | 15 | 1 | 748 | 1 | 744 | 0.0 | 1090 |
| Msa1275060 | AT3G13690.3 | 73.553 | 760 | 173 | 15 | 1 | 748 | 1 | 744 | 0.0 | 1090 |
| Msa1275060 | AT1G55200.3 | 68.567 | 719 | 174 | 17 | 1 | 710 | 1 | 676 | 0.0 | 950 |
| Msa1275060 | AT1G55200.4 | 68.567 | 719 | 174 | 17 | 1 | 710 | 1 | 676 | 0.0 | 950 |
| Msa1275060 | AT1G55200.1 | 68.567 | 719 | 174 | 17 | 1 | 710 | 1 | 676 | 0.0 | 950 |
| Msa1275060 | AT1G55200.2 | 68.567 | 719 | 174 | 17 | 1 | 710 | 1 | 676 | 0.0 | 950 |
| Msa1275060 | AT5G56790.1 | 60.725 | 662 | 236 | 7 | 28 | 682 | 23 | 667 | 0.0 | 783 |
| Msa1275060 | AT1G68690.1 | 49.555 | 337 | 157 | 4 | 344 | 676 | 323 | 650 | 1.16e-103 | 334 |
| Msa1275060 | AT1G68690.3 | 49.555 | 337 | 157 | 4 | 344 | 676 | 323 | 650 | 1.16e-103 | 334 |
Find 232 sgRNAs with CRISPR-Local
Find 389 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACGGTTATGGAAATGAG+GGG | 0.718438 | 8_3:-8915127 | None:intergenic |
| AAAAGCAATTCCGTGATCCA+CGG | 0.626544 | 8_3:-8917754 | None:intergenic |
| AAAGCTAGTGAAATGATGAA+AGG | 0.545382 | 8_3:+8915860 | Msa1275060:five_prime_UTR |
| AAAGCTGATGTTTATTCCTT+TGG | 0.259718 | 8_3:+8919512 | Msa1275060:CDS |
| AAATAGTGACCTCCCAGCCT+CGG | 0.504614 | 8_3:-8920091 | None:intergenic |
| AACCTATAAATGATCATGTG+AGG | 0.570637 | 8_3:+8915600 | Msa1275060:five_prime_UTR |
| AACCTGCTTCCTATTGGGAT+AGG | 0.611778 | 8_3:+8920849 | Msa1275060:CDS |
| AAGCAAGAGAAAGGTTGTGA+TGG | 0.606710 | 8_3:+8916338 | Msa1275060:CDS |
| AAGCTAGTGAAATGATGAAA+GGG | 0.536539 | 8_3:+8915861 | Msa1275060:five_prime_UTR |
| AAGCTGATGTTTATTCCTTT+GGG | 0.282461 | 8_3:+8919513 | Msa1275060:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTTATAGGAATGAAGAAGAA+AGG | - | chr8_3:8915020-8915039 | None:intergenic | 30.0% | |
| TGTATTTGAGAAAACGGTTA+TGG | - | chr8_3:8915140-8915159 | None:intergenic | 30.0% | |
| ATGAAGGTCAAATACATCAT+TGG | - | chr8_3:8915387-8915406 | None:intergenic | 30.0% | |
| AACCTATAAATGATCATGTG+AGG | + | chr8_3:8915600-8915619 | Msa1275060:five_prime_UTR | 30.0% | |
| ACCTATAAATGATCATGTGA+GGG | + | chr8_3:8915601-8915620 | Msa1275060:five_prime_UTR | 30.0% | |
| GCTATACACAAATAGTATCT+TGG | - | chr8_3:8915668-8915687 | None:intergenic | 30.0% | |
| ACCTAAAAAAGCAACTTAGA+AGG | - | chr8_3:8915717-8915736 | None:intergenic | 30.0% | |
| AAAGCTAGTGAAATGATGAA+AGG | + | chr8_3:8915860-8915879 | Msa1275060:five_prime_UTR | 30.0% | |
| AAGCTAGTGAAATGATGAAA+GGG | + | chr8_3:8915861-8915880 | Msa1275060:five_prime_UTR | 30.0% | |
| GAGCATATATCTATCATACA+TGG | + | chr8_3:8916226-8916245 | Msa1275060:five_prime_UTR | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 8915006 | 8921318 | 8915006 | ID=Msa1275060;Name=Msa1275060 |
| chr8_3 | mRNA | 8915006 | 8921318 | 8915006 | ID=Msa1275060-mRNA-1;Parent=Msa1275060;Name=Msa1275060-mRNA-1;_AED=0.06;_eAED=0.06;_QI=618|1|1|1|0.72|0.66|12|402|755 |
| chr8_3 | exon | 8915006 | 8915206 | 8915006 | ID=Msa1275060-mRNA-1:exon:3722;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8915553 | 8915734 | 8915553 | ID=Msa1275060-mRNA-1:exon:3723;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8915845 | 8915962 | 8915845 | ID=Msa1275060-mRNA-1:exon:3724;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8916194 | 8916497 | 8916194 | ID=Msa1275060-mRNA-1:exon:3725;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8916656 | 8916807 | 8916656 | ID=Msa1275060-mRNA-1:exon:3726;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8916895 | 8916989 | 8916895 | ID=Msa1275060-mRNA-1:exon:3727;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8917306 | 8918345 | 8917306 | ID=Msa1275060-mRNA-1:exon:3728;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8918639 | 8918799 | 8918639 | ID=Msa1275060-mRNA-1:exon:3729;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8919038 | 8919114 | 8919038 | ID=Msa1275060-mRNA-1:exon:3730;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8919469 | 8919616 | 8919469 | ID=Msa1275060-mRNA-1:exon:3731;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8920046 | 8920195 | 8920046 | ID=Msa1275060-mRNA-1:exon:3732;Parent=Msa1275060-mRNA-1 |
| chr8_3 | exon | 8920659 | 8921318 | 8920659 | ID=Msa1275060-mRNA-1:exon:3733;Parent=Msa1275060-mRNA-1 |
| chr8_3 | five_prime_UTR | 8915006 | 8915206 | 8915006 | ID=Msa1275060-mRNA-1:five_prime_utr;Parent=Msa1275060-mRNA-1 |
| chr8_3 | five_prime_UTR | 8915553 | 8915734 | 8915553 | ID=Msa1275060-mRNA-1:five_prime_utr;Parent=Msa1275060-mRNA-1 |
| chr8_3 | five_prime_UTR | 8915845 | 8915962 | 8915845 | ID=Msa1275060-mRNA-1:five_prime_utr;Parent=Msa1275060-mRNA-1 |
| chr8_3 | five_prime_UTR | 8916194 | 8916310 | 8916194 | ID=Msa1275060-mRNA-1:five_prime_utr;Parent=Msa1275060-mRNA-1 |
| chr8_3 | CDS | 8916311 | 8916497 | 8916311 | ID=Msa1275060-mRNA-1:cds;Parent=Msa1275060-mRNA-1 |
| chr8_3 | CDS | 8916656 | 8916807 | 8916656 | ID=Msa1275060-mRNA-1:cds;Parent=Msa1275060-mRNA-1 |
| chr8_3 | CDS | 8916895 | 8916989 | 8916895 | ID=Msa1275060-mRNA-1:cds;Parent=Msa1275060-mRNA-1 |
| chr8_3 | CDS | 8917306 | 8918345 | 8917306 | ID=Msa1275060-mRNA-1:cds;Parent=Msa1275060-mRNA-1 |
| chr8_3 | CDS | 8918639 | 8918799 | 8918639 | ID=Msa1275060-mRNA-1:cds;Parent=Msa1275060-mRNA-1 |
| chr8_3 | CDS | 8919038 | 8919114 | 8919038 | ID=Msa1275060-mRNA-1:cds;Parent=Msa1275060-mRNA-1 |
| chr8_3 | CDS | 8919469 | 8919616 | 8919469 | ID=Msa1275060-mRNA-1:cds;Parent=Msa1275060-mRNA-1 |
| chr8_3 | CDS | 8920046 | 8920195 | 8920046 | ID=Msa1275060-mRNA-1:cds;Parent=Msa1275060-mRNA-1 |
| chr8_3 | CDS | 8920659 | 8920916 | 8920659 | ID=Msa1275060-mRNA-1:cds;Parent=Msa1275060-mRNA-1 |
| chr8_3 | three_prime_UTR | 8920917 | 8921318 | 8920917 | ID=Msa1275060-mRNA-1:three_prime_utr;Parent=Msa1275060-mRNA-1 |
| Gene Sequence |
>Msa1275060 ATGAGTCGAGAGCAGCAAAAGAGGGGGAAGCAAGAGAAAGGTTGTGATGGTGCTGAGAAGGTCATTGTTGCTGTTAAGGCATCGACAAAGGAAATTCCGAAGACAGCTCTTGTTTGGTCACTGACTCATGTTGTTCAACCTGGTGATTGCATTACACTTCTAGTGGTTGTTCCTTCACAAAATTCAGGTAGAAAATGGGGTTTTCCAAGATTTGCTGGTGACTGTGCCGGTGGTATTAAGAAGTATCCTCCAGGAACAATTTTAGAGCAGAAGAGTGATATCACCGATTCTTGCTCTCAGATGATCCTTCAGCTCCATGATGTCTATGATCCAAATAAGATAAATGTCAGGATTAAAATTGTTTCCGGATCACCTTGTGGATCAGTGGCTGCAGAAGCCAAGAAAGGACAAGCTAGTTGGGTTGTATTAGACAAACATCTCAAACCTGAGGAGAAGAAATGCATGGAAGAGCTGCAGTGTAACATTGTTGTTATGAAGCGTGCTCAACCGAAAGTACTTCGCTTGAACTTGATTGGACCAAAAAAGAAGGAAGTTGAAGAAGCATGTTCATCACCTTCTGTGCAAGGCGATATGCTTGAAAAGCAAACCAAAAATAAGATTGATTCTTTAGTAGATTCTATCAAAGGACCAAATGTTACTCCAACTAGCAGCCCTGAGTTAGGTACACCATTCACTACTACTACTGAGGCCGGTACTTCATCAGCTTCAAGCTCTGATCCTGGAACATCACCGTTCTTTATCTCAATGAATGGTGAGTCGAAGAAAGAGGAAACCATCAAGGAAAGTCAAGAACTTTGTGATACTAATTCAGACACAGAAAGTGAAAGTCTGTCCACATCTTCTGCTAGCTTTAGATACCAGCCGTGGATCACGGAATTGCTTTTACATCAACAATCATCTCAACGCAACGAGGAAAGATCAGAGCCATATCACGGTATGCCTCAAGCAACTACAACCAAAGCCTTGCTAGAAAAATTTTCTAGGCTTGATAGAGGAGCTGAAATTGAAATGTCGGCAGCATATAGGAAGGACTCGGATTTTAGTGGTAATTTAAGAGAAGCAATAGCATTATCAGGAAATGCTCCACTTGGTCCACCTCCATTGTGTTCAATATGTCAACACAAGGCACCTGTATTTGGAAAACCTCCTAGGTGGTTCAGCTATGCTGAGTTGGAACTCGCTACCGGTGGATTTTCACAAGCTAACTTCTTGGCAGAAGGAGGGTATGGTTCTGTTCACCGAGGGGTTCTACCAGAAGGACAGGTCATTGCTGTCAAGCAACATAAATTGGCTAGTTCTCAAGGCGATGTTGAGTTTTGCTCCGAGGTAGAAGTTCTGAGCTGTGCTCAGCACCGAAATGTTGTTATGCTGATTGGATTCTGTATAGAGGATAAGAGAAGGCTACTGGTTTATGAGTACATATGCAATGGATCATTGGATTCACATTTATATGGGCGGCAAAGGGATCCATTAGAATGGTCCGCACGACAGAAAATTGCTGTTGGAGCTGCTCGTGGGTTAAGGTATCTTCATGAAGAGTGCAGAGTAGGTTGTATCATCCACCGTGACATGAGGCCAAACAATATTCTCATAACTCATGATTTTGAACCTCTGGTTGGTGATTTTGGACTGGCAAGGTGGCAGCCTGATGGAGACATGGGTGTGGACACTAGAGTAATTGGAACATTTGGGTATTTGGCTCCTGAATATGCTCAAAGTGGCCAAATCACTGAAAAAGCTGATGTTTATTCCTTTGGGGTGGTACTGGTGGAGCTTGTTACTGGTAGAAAAGCTGTTGATCTCACCAGGCCCAAGGGACAACAGTGCCTTGCTGAGTGGGCACGACCACTATTAGAAGAATATGCTATTGACGAACTGATTGACCCGAGGCTGGGAGGTCACTATTTAGAACATGAGATCTATTGCATGCTTCATGCTGCTTCATTATGCATAAGGAGAGATCCTCATTCTAGACCACGGATGTCACAGGTTCTCCGAATTCTAGAGGGCGATATGGTGATGGACGCAAATTACATTTCAACTCCAGGCTATGATGTTGGAAACAGGAGCGGTCGCCTTTGGTCAGAGCCACTGCAGAGGCAGCACCATTACAGTGGTCCCCTCTTAGAAGATTCATTGGAGTCATCATTCAGTGGAAATCTATCTCTTGACAAGTACAAACCTGCTTCCTATTGGGATAGGAATAGGGACAAGCCAAGAAGGGCATCATGTGAAGGTGATACTTGA |
| Protein sequence |
>Msa1275060 MSREQQKRGKQEKGCDGAEKVIVAVKASTKEIPKTALVWSLTHVVQPGDCITLLVVVPSQNSGRKWGFPRFAGDCAGGIKKYPPGTILEQKSDITDSCSQMILQLHDVYDPNKINVRIKIVSGSPCGSVAAEAKKGQASWVVLDKHLKPEEKKCMEELQCNIVVMKRAQPKVLRLNLIGPKKKEVEEACSSPSVQGDMLEKQTKNKIDSLVDSIKGPNVTPTSSPELGTPFTTTTEAGTSSASSSDPGTSPFFISMNGESKKEETIKESQELCDTNSDTESESLSTSSASFRYQPWITELLLHQQSSQRNEERSEPYHGMPQATTTKALLEKFSRLDRGAEIEMSAAYRKDSDFSGNLREAIALSGNAPLGPPPLCSICQHKAPVFGKPPRWFSYAELELATGGFSQANFLAEGGYGSVHRGVLPEGQVIAVKQHKLASSQGDVEFCSEVEVLSCAQHRNVVMLIGFCIEDKRRLLVYEYICNGSLDSHLYGRQRDPLEWSARQKIAVGAARGLRYLHEECRVGCIIHRDMRPNNILITHDFEPLVGDFGLARWQPDGDMGVDTRVIGTFGYLAPEYAQSGQITEKADVYSFGVVLVELVTGRKAVDLTRPKGQQCLAEWARPLLEEYAIDELIDPRLGGHYLEHEIYCMLHAASLCIRRDPHSRPRMSQVLRILEGDMVMDANYISTPGYDVGNRSGRLWSEPLQRQHHYSGPLLEDSLESSFSGNLSLDKYKPASYWDRNRDKPRRASCEGDT* |
