AlfalfaGEDB | Msa1281500

M. sativa cultivar zhongmu-4 / Msa1281500

KEGG: K08064
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0008150 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0010154 GO:0010262 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0045892 GO:0045934 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
PFAM: PF02045.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1281500	AFK49649.1	95.395	304	14	0	1	304	1	304	0.0	602

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1281500	sp\|Q84ZN6\|CESA8_ORYSJ	80.000	95	18	1	298	391	987	1081	2.12e-43	165

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1281500	I3TAV7	95.395	304	14	0	1	304	1	304	0.0	602

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TFs/TRs:

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Gene ID	Type	Classification
Msa1281500	TF	NF-YA

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1281500	MtrunA17_Chr8g0352251	95.189	291	14	0	1	291	1	291	0.0	575
Msa1281500	MtrunA17_Chr8g0352231	86.316	95	12	1	298	391	984	1078	1.34e-47	174
Msa1281500	MtrunA17_Chr3g0088821	82.105	95	16	1	298	391	984	1078	5.11e-46	169
Msa1281500	MtrunA17_Chr3g0103391	45.933	209	91	5	21	209	20	226	2.81e-45	159
Msa1281500	MtrunA17_Chr4g0073711	68.750	96	30	0	296	391	943	1038	9.53e-41	154
Msa1281500	MtrunA17_Chr7g0267731	72.414	87	23	1	306	391	900	986	6.92e-38	145
Msa1281500	MtrunA17_Chr2g0296991	64.583	96	34	0	296	391	949	1044	1.08e-36	142
Msa1281500	MtrunA17_Chr2g0300261	49.669	151	67	4	114	259	33	179	5.42e-36	131
Msa1281500	MtrunA17_Chr3g0078671	60.194	103	34	1	296	391	947	1049	6.80e-36	139
Msa1281500	MtrunA17_Chr2g0292411	71.111	90	26	0	127	216	86	175	2.40e-35	129

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1281500	AT5G05170.1	80.000	95	18	1	298	391	971	1065	2.61e-44	164
Msa1281500	AT5G17420.1	83.333	78	13	0	314	391	949	1026	1.70e-40	153
Msa1281500	AT5G44030.3	82.051	78	14	0	314	391	693	770	4.23e-39	149
Msa1281500	AT5G44030.2	82.051	78	14	0	314	391	1001	1078	4.54e-39	149
Msa1281500	AT5G44030.1	82.051	78	14	0	314	391	972	1049	4.71e-39	149
Msa1281500	AT5G12840.4	38.750	240	120	7	29	245	27	262	2.40e-38	139
Msa1281500	AT5G12840.2	38.750	240	120	7	29	245	27	262	2.40e-38	139
Msa1281500	AT5G12840.5	47.917	144	74	1	103	245	120	263	9.66e-38	137
Msa1281500	AT5G12840.3	47.917	144	74	1	103	245	120	263	9.66e-38	137
Msa1281500	AT5G12840.1	47.917	144	74	1	103	245	120	263	9.66e-38	137

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Find 124 sgRNAs with CRISPR-Local

Find 285 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTGTGAAAGGATGCC+AGG	0.439397	8_3:-21118666	Msa1281500:exon
AAACAGGAGGGTGACTTCTT+TGG	0.313426	8_3:-21116018	Msa1281500:CDS
AAAGCTGTGAAAGGATGCCA+GGG	0.629084	8_3:-21118665	Msa1281500:exon
AACAATACTGGTGCAACATC+GGG	0.490937	8_3:-21116165	Msa1281500:CDS
AACAGTGGTTACCAATCATG+GGG	0.667481	8_3:-21115222	Msa1281500:intron
AACATGCTTTGAGAAGGGCA+AGG	0.629479	8_3:-21116260	Msa1281500:CDS
AACCCATAATAGAGAGAAAA+TGG	0.274581	8_3:+21115071	None:intergenic
AAGAAAAGTTTACCAAAGAG+CGG	0.667745	8_3:+21115198	None:intergenic
AAGAGAATATAAGAATAACA+AGG	0.662027	8_3:+21119390	None:intergenic
AATCGACCAGACTACAACAA+TGG	0.628569	8_3:+21115104	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGGATCTCTTTCATTTAATT+AGG	-	chr8_3:21115403-21115422	Msa1281500:intron	30.0%
TTGACAAAGCTCATATTGTT+AGG	-	chr8_3:21115760-21115779	Msa1281500:intron	30.0%
TAGAAGAACTTACTATTGGA+AGG	+	chr8_3:21116291-21116310	None:intergenic	30.0%
AAACACCCTTTACAAAATTG+TGG	+	chr8_3:21116371-21116390	None:intergenic	30.0%
TCTCAAATTATTAGTGGTGT+CGG	+	chr8_3:21116661-21116680	None:intergenic	30.0%
TTATCAGTTAGAGAAAGTGA+AGG	+	chr8_3:21117085-21117104	None:intergenic	30.0%
TCTAGATACCAAATTGCAAA+TGG	+	chr8_3:21117260-21117279	None:intergenic	30.0%
TCTAGATTTGTCAAACCAAT+TGG	-	chr8_3:21117274-21117293	Msa1281500:intron	30.0%
TTTCCATGAAAAAGTCCAAT+TGG	+	chr8_3:21117292-21117311	None:intergenic	30.0%
CCTTATTCAAATGCACAATT+CGG	-	chr8_3:21117772-21117791	Msa1281500:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	21115005	21120567	21115005	ID=Msa1281500;Name=Msa1281500
chr8_3	mRNA	21115005	21120567	21115005	ID=Msa1281500-mRNA-1;Parent=Msa1281500;Name=Msa1281500-mRNA-1;_AED=0.16;_eAED=0.16;_QI=1310\|0.71\|0.62\|1\|0.85\|0.75\|8\|0\|391
chr8_3	exon	21115005	21115239	21115005	ID=Msa1281500-mRNA-1:exon:7724;Parent=Msa1281500-mRNA-1
chr8_3	exon	21115798	21115867	21115798	ID=Msa1281500-mRNA-1:exon:7723;Parent=Msa1281500-mRNA-1
chr8_3	exon	21115998	21116307	21115998	ID=Msa1281500-mRNA-1:exon:7722;Parent=Msa1281500-mRNA-1
chr8_3	exon	21116707	21116871	21116707	ID=Msa1281500-mRNA-1:exon:7721;Parent=Msa1281500-mRNA-1
chr8_3	exon	21117744	21117812	21117744	ID=Msa1281500-mRNA-1:exon:7720;Parent=Msa1281500-mRNA-1
chr8_3	exon	21117883	21117998	21117883	ID=Msa1281500-mRNA-1:exon:7719;Parent=Msa1281500-mRNA-1
chr8_3	exon	21118463	21118690	21118463	ID=Msa1281500-mRNA-1:exon:7718;Parent=Msa1281500-mRNA-1
chr8_3	exon	21119275	21120567	21119275	ID=Msa1281500-mRNA-1:exon:7717;Parent=Msa1281500-mRNA-1
chr8_3	five_prime_UTR	21119275	21120567	21119275	ID=Msa1281500-mRNA-1:five_prime_utr;Parent=Msa1281500-mRNA-1
chr8_3	five_prime_UTR	21118674	21118690	21118674	ID=Msa1281500-mRNA-1:five_prime_utr;Parent=Msa1281500-mRNA-1
chr8_3	CDS	21118463	21118673	21118463	ID=Msa1281500-mRNA-1:cds;Parent=Msa1281500-mRNA-1
chr8_3	CDS	21117883	21117998	21117883	ID=Msa1281500-mRNA-1:cds;Parent=Msa1281500-mRNA-1
chr8_3	CDS	21117744	21117812	21117744	ID=Msa1281500-mRNA-1:cds;Parent=Msa1281500-mRNA-1
chr8_3	CDS	21116707	21116871	21116707	ID=Msa1281500-mRNA-1:cds;Parent=Msa1281500-mRNA-1
chr8_3	CDS	21115998	21116307	21115998	ID=Msa1281500-mRNA-1:cds;Parent=Msa1281500-mRNA-1
chr8_3	CDS	21115798	21115867	21115798	ID=Msa1281500-mRNA-1:cds;Parent=Msa1281500-mRNA-1
chr8_3	CDS	21115005	21115239	21115005	ID=Msa1281500-mRNA-1:cds;Parent=Msa1281500-mRNA-1

Gene Sequence

>Msa1281500

ATGCCAGGGAAACCAGAAACTGATGATTGGAGAATAAAACATAGTGAGCAGGTTCAGTTGCCATCTTCCATTTACTCTCATCATCAGCCTTGGTGGCTTGAAAATGGCTCCAAACCATCTTCAACTGATCAGCTAAATAGTTCAATCATGAATGATGTCACACATTCAGAGCCTTTGGTTTCTCCTTCACTTTCTCTAACTGATAACTCAGGTGGGGACGTTGCCAAGGAGCACCGAAACATCAAACATGCCCTGTCCTCAACCGCATTTACCATGGACAAACACCTTGGTCCAAATTCGCAGACGGAACTTGTTGGTCATTCAATTGTTTTAACATCTCCTTATTCAAATGCACAATTCGGCCAAATCTTGAATGCTTATGGACAACAAGCTATGATGAACCCTCAGCTATACCAAATGCATCATGCTAGAATGCTTTTGCCGCTTAAAATGGAAGAGGAACCTGTGTATGTCAATGCAAAGCAGTATCATGGTATTTTGAGACGAAGACAATCGCGTGCAAAGGCTGAGCTTGAAAAGAAAGTTATTAAAGTTAGAAAGCCATATCTTCATGAATCCCGTCACCAACATGCTTTGAGAAGGGCAAGGGGAAATGGCGGTCGTTTCCTCAATACAAAGAAGCTCGAACACAACGATTCTAATGCTGCTCTAGAAAAAGGCAACAATACTGGTGCAACATCGGGTACCAACAATGAGAATCAAGGCTCATCTAATGTGTCGCAGCACATGCACAAAATGCAGGGTTTCAACATCAGTTACCATGATGGAAATGACTTTACAGCACTATGTCATTCACAGGCAAATGGAAAACAGGAGGGTGACTTCTTTGGTAAAAAGATTGAATCAAATGGTGAAGATGAAGGGCTTTGCCTCATCCAACTCATGATGCTGTATATCAATGGCAACTCATTCTTGGCTAATGGTTACCAATCATGGGGTCCGCTCTTTGGTAAACTTTTCTTTGCATTTTGGGTTATCGTTCATCTCTACCCTTTCCTTAAAGGTCTTATGGGACGCCAGAATCGAACGCCGACCATTGTTGTAGTCTGGTCGATTCTTCTTGCGTCCATTTTCTCTCTATTATGGGTTCGAGTTGATCCGTTTACTACAAGAGTCACTGGTCCTAAAGCTGAAGAGTGTGGAATTAACTGCTAG

Protein sequence

>Msa1281500

MPGKPETDDWRIKHSEQVQLPSSIYSHHQPWWLENGSKPSSTDQLNSSIMNDVTHSEPLVSPSLSLTDNSGGDVAKEHRNIKHALSSTAFTMDKHLGPNSQTELVGHSIVLTSPYSNAQFGQILNAYGQQAMMNPQLYQMHHARMLLPLKMEEEPVYVNAKQYHGILRRRQSRAKAELEKKVIKVRKPYLHESRHQHALRRARGNGGRFLNTKKLEHNDSNAALEKGNNTGATSGTNNENQGSSNVSQHMHKMQGFNISYHDGNDFTALCHSQANGKQEGDFFGKKIESNGEDEGLCLIQLMMLYINGNSFLANGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVVWSILLASIFSLLWVRVDPFTTRVTGPKAEECGINC*