AlfalfaGEDB | Msa1282980

M. sativa cultivar zhongmu-4 / Msa1282980

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1282980	sp\|Q1PEM5\|PERK3_ARATH	48.627	255	111	5	95	329	144	398	4.37e-69	227

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1282980	A0A396GJR5	95.327	321	15	0	10	330	1	321	0.0	650

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1282980	MtrunA17_Chr8g0354651	95.327	321	15	0	10	330	1	321	0.0	650
Msa1282980	MtrunA17_Chr3g0080901	73.663	243	64	0	87	329	94	336	3.57e-130	381
Msa1282980	MtrunA17_Chr3g0080881	68.182	264	81	3	75	335	25	288	7.82e-122	356
Msa1282980	MtrunA17_Chr2g0330471	55.466	247	101	5	92	329	377	623	1.20e-87	277
Msa1282980	MtrunA17_Chr8g0341881	55.466	247	101	5	92	329	375	621	7.39e-86	274
Msa1282980	MtrunA17_Chr8g0389251	54.251	247	104	5	92	329	374	620	5.86e-84	267
Msa1282980	MtrunA17_Chr3g0093601	51.383	253	121	2	79	329	64	316	1.07e-81	253
Msa1282980	MtrunA17_Chr3g0093591	52.000	250	115	4	84	329	95	343	1.05e-79	249
Msa1282980	MtrunA17_Chr3g0093611	49.398	249	116	3	91	329	35	283	3.97e-78	243
Msa1282980	MtrunA17_Chr5g0413511	50.000	228	107	4	109	329	47	274	2.15e-70	224

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1282980	AT5G56790.1	54.656	247	103	5	92	329	360	606	3.46e-85	271
Msa1282980	AT1G55200.3	54.435	248	102	6	92	329	349	595	4.55e-83	265
Msa1282980	AT1G55200.4	54.435	248	102	6	92	329	349	595	4.55e-83	265
Msa1282980	AT1G55200.1	54.435	248	102	6	92	329	349	595	4.55e-83	265
Msa1282980	AT1G55200.2	54.435	248	102	6	92	329	349	595	4.55e-83	265
Msa1282980	AT3G13690.2	53.629	248	104	6	92	329	381	627	3.10e-81	262
Msa1282980	AT3G13690.1	53.629	248	104	6	92	329	381	627	3.10e-81	262
Msa1282980	AT3G13690.3	53.629	248	104	6	92	329	381	627	3.10e-81	262
Msa1282980	AT3G24540.1	48.627	255	111	5	95	329	144	398	1.91e-70	227
Msa1282980	AT5G38560.1	42.140	299	154	7	42	329	266	556	1.21e-68	227

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Find 66 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATACACATCTGACT+TGG	0.618312	8_3:-24115259	None:intergenic
AAAGATCTAGATATGGCAAG+AGG	0.653248	8_3:+24114070	Msa1282980:CDS
AACCTCTTAATTGGTCAGCC+AGG	0.519014	8_3:+24114892	Msa1282980:CDS
AAGAACTATTTGTCAGAAGG+TGG	0.558282	8_3:+24114316	Msa1282980:CDS
AAGTCAGATGTGTATTCTTT+TGG	0.289391	8_3:+24115261	Msa1282980:CDS
ACACAAAAGTCTTGTGGGAT+GGG	0.501905	8_3:+24115338	Msa1282980:CDS
ACAGATGTTGTTGGAACATT+AGG	0.251263	8_3:+24115195	Msa1282980:CDS
ACTCCAGGAAACCTCTTAAT+TGG	0.328045	8_3:+24114883	Msa1282980:CDS
ACTTTCATGCAGAACACTCC+AGG	0.522767	8_3:+24114868	Msa1282980:intron
AGCCAGGATCAAAGTGGCAA+TGG	0.512784	8_3:+24114908	Msa1282980:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAAGCAATACATGAAAATG+TGG	+	chr8_3:24114453-24114472	Msa1282980:CDS	30.0%
TTTCACTAGGTAGATATTTG+TGG	+	chr8_3:24114700-24114719	Msa1282980:intron	30.0%
GATATTTGTGGAGATGATTT+AGG	+	chr8_3:24114712-24114731	Msa1282980:intron	30.0%
CTTCATAATCATGTGTAACA+AGG	-	chr8_3:24115004-24115023	None:intergenic	30.0%
TACACATGATTATGAAGCAA+TGG	+	chr8_3:24115007-24115026	Msa1282980:CDS	30.0%
AAAAGAATACACATCTGACT+TGG	-	chr8_3:24115262-24115281	None:intergenic	30.0%
CAAAGCAATACATGAAAATG+TGG	+	chr8_3:24114453-24114472	Msa1282980:CDS	30.0%
TTTCACTAGGTAGATATTTG+TGG	+	chr8_3:24114700-24114719	Msa1282980:intron	30.0%
GATATTTGTGGAGATGATTT+AGG	+	chr8_3:24114712-24114731	Msa1282980:intron	30.0%
CTTCATAATCATGTGTAACA+AGG	-	chr8_3:24115004-24115023	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	24113947	24115736	24113947	ID=Msa1282980;Name=Msa1282980
chr8_3	mRNA	24113947	24115736	24113947	ID=Msa1282980-mRNA-1;Parent=Msa1282980;Name=Msa1282980-mRNA-1;_AED=0.11;_eAED=0.14;_QI=0\|0\|0\|1\|0.66\|0.5\|4\|0\|340
chr8_3	exon	24113947	24114565	24113947	ID=Msa1282980-mRNA-1:exon:8543;Parent=Msa1282980-mRNA-1
chr8_3	exon	24114880	24115028	24114880	ID=Msa1282980-mRNA-1:exon:8544;Parent=Msa1282980-mRNA-1
chr8_3	exon	24115144	24115359	24115144	ID=Msa1282980-mRNA-1:exon:8545;Parent=Msa1282980-mRNA-1
chr8_3	exon	24115698	24115736	24115698	ID=Msa1282980-mRNA-1:exon:8546;Parent=Msa1282980-mRNA-1
chr8_3	CDS	24113947	24114565	24113947	ID=Msa1282980-mRNA-1:cds;Parent=Msa1282980-mRNA-1
chr8_3	CDS	24114880	24115028	24114880	ID=Msa1282980-mRNA-1:cds;Parent=Msa1282980-mRNA-1
chr8_3	CDS	24115144	24115359	24115144	ID=Msa1282980-mRNA-1:cds;Parent=Msa1282980-mRNA-1
chr8_3	CDS	24115698	24115736	24115698	ID=Msa1282980-mRNA-1:cds;Parent=Msa1282980-mRNA-1

Gene Sequence

>Msa1282980

CCTGAATCCTCACCAAGTTTGTGGAGCATGAGAAACGCTGTTTTCCGCTCCAAATCGAGCTCTAATCTAACAAAGATTGTTTGTCATCAACATCATTGTCAAATAATACAAACACCTGAAGATAAAGATCTAGATATGGCAAGAGGCTATTGCATATTACCAACTCCTGATAATCAAAAGACAAGAGTTCTTGAAATGCATCACAAAGATTCAACATTTAAACATATCAGAAACCATAAAACATATCAGATCGTTGAAAATTTTAAAAATTCTGTGTGCTCAGTGTGTGAGAACAAAAGGCCAAAATTTGAATTGATGAAAGAATTCACATATGGAGAGTTGCATGAAGCAACGCATGGTTTTTCGGCAAAGAACTATTTGTCAGAAGGTGGTTTCGGTTCTGTTTATTGGGGAAAGATGCAAGGGCTCAAGATCGCTGTTAAGAAACATAAATTTGCAAGCCTTCAAGGAGAGAAGGAATTCAAATCTGAAGTTAATGCACTCAACAAAGCAATACATGAAAATGTGGTCATGCTGCTAGGATCTTGTTCGGAGGGAAAGAACAGATTTCTTGTATATGAATTTGTTTGCAATGGCTCACTTGATCAACATTTATCGCAACACTCCAGGAAACCTCTTAATTGGTCAGCCAGGATCAAAGTGGCAATGGGGGCTGCAAAAGGCTTGTTGTATTTGCATGAGAATAACATCATACACAGAGATATGAGACCAAGTAACATCCTTGTTACACATGATTATGAAGCAATGATTGGAGATTTTGGTCTAGCTAGGACTGAACAAATGGACTCAGTGTACTCAACAGATGTTGTTGGAACATTAGGGTATATGGCACCAGAATATGCAGAATCTGGTAAAGTCTCAACCAAGTCAGATGTGTATTCTTTTGGAGTGGTGCTTCTTCAGTTAATCACAGGAATGAGGACCACAGACAAAAGGATTGGACACAAAAGTCTTGTGGGATGGGCCACTACTGAAGGAAAGGAACTATCCAGATTTAATTGA

Protein sequence

>Msa1282980

PESSPSLWSMRNAVFRSKSSSNLTKIVCHQHHCQIIQTPEDKDLDMARGYCILPTPDNQKTRVLEMHHKDSTFKHIRNHKTYQIVENFKNSVCSVCENKRPKFELMKEFTYGELHEATHGFSAKNYLSEGGFGSVYWGKMQGLKIAVKKHKFASLQGEKEFKSEVNALNKAIHENVVMLLGSCSEGKNRFLVYEFVCNGSLDQHLSQHSRKPLNWSARIKVAMGAAKGLLYLHENNIIHRDMRPSNILVTHDYEAMIGDFGLARTEQMDSVYSTDVVGTLGYMAPEYAESGKVSTKSDVYSFGVVLLQLITGMRTTDKRIGHKSLVGWATTEGKELSRFN*