AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1283750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1283750 MtrunA17_Chr8g0355261 91.028 457 17 5 1 451 1 439 0.0 847
Msa1283750 MtrunA17_Chr6g0453321 36.869 396 174 12 107 450 123 494 1.48e-64 216
Msa1283750 MtrunA17_Chr4g0042481 37.076 383 169 14 109 449 111 463 3.17e-62 209
Msa1283750 MtrunA17_Chr7g0217581 32.480 508 250 20 11 450 100 582 2.79e-60 206
Msa1283750 MtrunA17_Chr3g0092111 34.112 428 204 15 66 450 72 464 3.94e-57 195
Msa1283750 MtrunA17_Chr7g0254161 36.364 374 170 11 108 451 89 424 1.04e-55 190
Msa1283750 MtrunA17_Chr5g0417561 32.620 374 155 13 83 451 82 363 3.23e-41 150
Msa1283750 MtrunA17_Chr5g0417551 30.000 460 208 19 14 451 4 371 1.03e-37 141
Msa1283750 MtrunA17_Chr6g0460721 32.425 367 162 14 16 346 13 329 5.75e-37 138
Msa1283750 MtrunA17_Chr6g0460731 29.219 397 193 13 13 360 10 367 4.42e-35 134
Msa1283750 MtrunA17_Chr2g0283811 77.419 62 14 0 112 173 39 100 2.37e-32 120
Msa1283750 MtrunA17_Chr5g0417571 74.627 67 17 0 109 175 127 193 2.51e-31 122
Msa1283750 MtrunA17_Chr5g0417601 62.338 77 29 0 97 173 117 193 1.84e-29 118
Msa1283750 MtrunA17_Chr5g0417601 32.105 190 72 9 274 451 237 381 7.11e-13 70.1
Msa1283750 MtrunA17_Chr4g0010151 67.797 59 19 0 116 174 78 136 3.82e-25 106
Msa1283750 MtrunA17_Chr3g0127201 59.701 67 27 0 109 175 16 82 4.60e-25 104
Msa1283750 MtrunA17_Chr2g0308011 74.000 50 13 0 119 168 36 85 9.35e-25 101
Msa1283750 MtrunA17_Chr4g0061761 71.698 53 15 0 118 170 38 90 2.74e-24 102
Msa1283750 MtrunA17_Chr8g0346551 61.290 62 24 0 115 176 19 80 4.75e-24 100
Msa1283750 MtrunA17_Chr2g0328021 65.000 60 21 0 120 179 62 121 5.64e-24 101
Msa1283750 MtrunA17_Chr8g0374111 60.606 66 25 1 120 185 29 93 6.12e-24 99.8
Msa1283750 MtrunA17_Chr4g0031411 66.102 59 20 0 116 174 71 129 8.67e-24 101
Msa1283750 MtrunA17_Chr2g0326001 63.333 60 22 0 115 174 35 94 1.32e-23 100
Msa1283750 MtrunA17_Chr8g0374251 72.222 54 15 0 120 173 68 121 1.59e-23 101
Msa1283750 MtrunA17_Chr8g0341671 69.231 52 16 0 120 171 20 71 3.94e-23 97.1
Msa1283750 MtrunA17_Chr8g0367161 46.914 81 43 0 94 174 6 86 9.78e-23 98.6
Msa1283750 MtrunA17_Chr7g0237921 69.811 53 16 0 122 174 78 130 1.30e-22 98.6
Msa1283750 MtrunA17_Chr7g0223311 49.451 91 35 2 84 174 52 131 1.33e-22 99.0
Msa1283750 MtrunA17_Chr1g0188851 56.452 62 27 0 114 175 37 98 1.88e-22 97.1
Msa1283750 MtrunA17_Chr4g0047001 67.925 53 17 0 122 174 57 109 5.90e-22 97.1
Msa1283750 MtrunA17_Chr5g0411951 65.455 55 19 0 116 170 39 93 6.13e-22 96.3
Msa1283750 MtrunA17_Chr4g0030051 60.000 65 25 1 109 172 29 93 2.92e-21 93.6
Msa1283750 MtrunA17_Chr3g0117601 53.521 71 32 1 101 170 41 111 4.65e-21 94.0
Msa1283750 MtrunA17_Chr3g0126201 64.151 53 19 0 122 174 53 105 6.09e-21 93.6
Msa1283750 MtrunA17_Chr2g0282441 68.000 50 16 0 122 171 27 76 1.06e-20 92.8
Msa1283750 MtrunA17_Chr2g0282511 68.000 50 16 0 119 168 44 93 1.15e-20 92.4
Msa1283750 MtrunA17_Chr1g0177251 64.151 53 19 0 122 174 14 66 1.26e-20 91.7
Msa1283750 MtrunA17_Chr4g0047761 65.455 55 18 1 120 174 72 125 1.71e-20 92.0
Msa1283750 MtrunA17_Chr2g0282051 66.038 53 17 1 122 174 48 99 2.37e-20 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1283750 AT5G39660.3 37.186 398 200 13 71 450 91 456 8.09e-61 205
Msa1283750 AT5G39660.1 37.186 398 200 13 71 450 91 456 8.09e-61 205
Msa1283750 AT5G39660.2 37.186 398 200 13 71 450 91 456 8.09e-61 205
Msa1283750 AT3G47500.1 33.402 485 239 20 11 451 4 448 1.58e-56 194
Msa1283750 AT1G69570.1 29.202 476 221 14 11 451 3 397 1.98e-45 163
Msa1283750 AT1G26790.1 30.747 348 153 14 113 451 104 372 4.86e-35 134
Msa1283750 AT1G29160.1 69.863 73 22 0 101 173 43 115 4.70e-34 126
Msa1283750 AT5G62430.1 62.921 89 32 1 85 173 20 107 5.22e-34 129
Msa1283750 AT5G62430.1 40.777 103 42 4 357 451 207 298 4.31e-11 64.3
Msa1283750 AT2G34140.1 76.667 60 14 0 114 173 52 111 3.59e-32 120
Msa1283750 AT5G60850.1 66.667 69 21 1 120 188 51 117 1.51e-26 109
Msa1283750 AT3G55370.2 68.333 60 19 0 116 175 72 131 5.83e-25 105
Msa1283750 AT3G55370.1 68.333 60 19 0 116 175 72 131 5.99e-25 105
Msa1283750 AT3G55370.3 68.333 60 19 0 116 175 117 176 8.78e-25 105
Msa1283750 AT1G51700.1 68.421 57 17 1 120 175 33 89 1.65e-24 100
Msa1283750 AT2G37590.1 65.000 60 21 0 116 175 85 144 1.83e-24 103
Msa1283750 AT2G28810.2 69.643 56 17 0 116 171 73 128 2.94e-24 103
Msa1283750 AT2G28810.1 69.643 56 17 0 116 171 90 145 4.92e-24 102
Msa1283750 AT3G45610.1 50.633 79 34 1 102 175 17 95 5.41e-24 100
Msa1283750 AT5G60200.1 61.667 60 23 0 116 175 51 110 8.20e-24 100
Msa1283750 AT3G61850.2 51.765 85 39 1 115 197 57 141 1.18e-23 100
Msa1283750 AT3G61850.3 51.765 85 39 1 115 197 57 141 1.18e-23 100
Msa1283750 AT3G61850.1 51.765 85 39 1 115 197 69 153 1.20e-23 100
Msa1283750 AT5G65590.1 51.685 89 40 1 87 175 11 96 1.39e-23 101
Msa1283750 AT1G07640.1 69.091 55 17 0 120 174 21 75 1.68e-23 100
Msa1283750 AT3G61850.5 51.765 85 39 1 115 197 57 141 1.84e-23 100
Msa1283750 AT4G38000.1 68.966 58 17 1 119 176 40 96 1.86e-23 99.4
Msa1283750 AT3G61850.4 51.765 85 39 1 115 197 69 153 1.87e-23 100
Msa1283750 AT3G21270.1 69.811 53 16 0 120 172 29 81 2.17e-23 97.8
Msa1283750 AT3G50410.1 50.000 80 30 1 105 174 5 84 2.21e-23 99.4
Msa1283750 AT1G07640.2 69.091 55 17 0 120 174 77 131 3.10e-23 100
Msa1283750 AT1G07640.3 69.091 55 17 0 120 174 85 139 3.88e-23 100
Msa1283750 AT5G02460.1 66.102 59 20 0 116 174 91 149 5.71e-23 100
Msa1283750 AT4G24060.1 56.061 66 29 0 109 174 42 107 5.72e-23 99.8
Msa1283750 AT1G64620.1 61.667 60 23 0 109 168 38 97 1.50e-22 99.0
Msa1283750 AT4G00940.1 50.000 78 39 0 115 192 63 140 2.98e-22 97.1
Msa1283750 AT4G00940.3 50.000 78 39 0 115 192 63 140 2.98e-22 97.1
Msa1283750 AT4G00940.2 50.000 78 39 0 115 192 63 140 2.98e-22 97.1
Msa1283750 AT2G28510.1 68.519 54 17 0 122 175 50 103 3.42e-22 96.7
Msa1283750 AT1G47655.1 69.388 49 15 0 120 168 29 77 9.35e-22 93.6
Msa1283750 AT1G28310.1 65.455 55 19 0 120 174 27 81 1.28e-21 95.5
Msa1283750 AT1G28310.2 65.455 55 19 0 120 174 51 105 1.86e-21 95.5
Msa1283750 AT5G66940.1 60.000 55 22 0 120 174 32 86 3.54e-21 92.4
Msa1283750 AT4G21050.1 43.956 91 49 1 105 193 9 99 3.86e-21 91.7
Msa1283750 AT2G46590.1 64.151 53 19 0 115 167 63 115 9.95e-21 93.6
Msa1283750 AT2G46590.2 64.151 53 19 0 115 167 75 127 1.03e-20 94.0
Msa1283750 AT3G52440.1 62.264 53 20 0 122 174 27 79 1.86e-20 90.9
Msa1283750 AT3G52440.2 62.264 53 20 0 122 174 46 98 2.14e-20 90.9
Msa1283750 AT1G21340.1 61.111 54 21 0 122 175 40 93 3.20e-20 90.5
Msa1283750 AT5G62940.1 65.306 49 17 0 122 170 75 123 1.72e-19 90.1
Msa1283750 AT4G21040.1 67.347 49 16 0 122 170 27 75 4.07e-18 83.6
Msa1283750 AT4G21080.1 65.306 49 17 0 122 170 27 75 2.12e-17 82.0
Msa1283750 AT4G21030.1 50.769 65 25 2 108 165 2 66 1.46e-12 66.6

Find 102 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACACTTCCTTTCATAAT+AGG 0.219092 8_3:-25380938 None:intergenic
ATACGATGAAGCCGAATATT+TGG 0.219567 8_3:+25380825 Msa1283750:CDS
TCCATTTGGCCAATCCAATT+TGG 0.234872 8_3:-25380671 None:intergenic
GAACTCTTAGCAGCCTCTTC+TGG 0.248749 8_3:-25380875 None:intergenic
GTTGTTGTTCTTGTTTAATA+TGG 0.278099 8_3:-25378619 None:intergenic
ATTGGGGCTGTATGCCAAAT+TGG 0.283292 8_3:+25380657 Msa1283750:CDS
TTGCTTATTTCTACGCTTAC+CGG 0.293241 8_3:-25380219 None:intergenic
GGACTTGACGTTATGTTATC+AGG 0.299089 8_3:-25380593 None:intergenic
GGTAATAGGTCACCAACTTT+AGG 0.303102 8_3:+25380770 Msa1283750:CDS
ACCGATGACACCTTCGTATT+GGG 0.307791 8_3:+25380640 Msa1283750:CDS
GGACAGCTGGTGGAGTAATT+AGG 0.315052 8_3:+25380180 Msa1283750:CDS
TTGATTGTCAGCATCTCTTT+TGG 0.322651 8_3:-25380024 None:intergenic
GTGGTACTTGTTCCGGTAAT+AGG 0.368265 8_3:+25380756 Msa1283750:CDS
TTGTGGATTAGCTTGTTTGC+TGG 0.369067 8_3:-25379910 None:intergenic
GCTAGCAGTGGTACTTGTTC+CGG 0.387977 8_3:+25380749 Msa1283750:CDS
TCTGTGATAGCTGCTGAATC+CGG 0.390319 8_3:+25380425 Msa1283750:CDS
GCCAAATTGGATTGGCCAAA+TGG 0.393333 8_3:+25380670 Msa1283750:CDS
AGCTGCTGAATCCGGGGAAA+TGG 0.396343 8_3:+25380433 Msa1283750:CDS
CTAACGATAATTCTTCCATT+TGG 0.399422 8_3:-25380685 None:intergenic
ATGGAAGAATTATCGTTAGT+TGG 0.407541 8_3:+25380689 Msa1283750:CDS
AAATGCCACCGGATTCCAAC+AGG 0.408721 8_3:-25380567 None:intergenic
GTACCGGTATTGTACATGTT+TGG 0.410276 8_3:-25379951 None:intergenic
GTAATTAGGAATGTTCCTAT+TGG 0.410965 8_3:+25380194 Msa1283750:CDS
GGTACTGCATCAAGGGTAAC+AGG 0.411943 8_3:-25380266 None:intergenic
CGAGAACTTGCATTTGACTT+AGG 0.412589 8_3:-25380974 None:intergenic
CTACGCTTACCGGCTCCAAT+AGG 0.417710 8_3:-25380209 None:intergenic
TACGATGAAGCCGAATATTT+GGG 0.418538 8_3:+25380826 Msa1283750:CDS
TAAAGTTGGTGACCTATTAC+CGG 0.424336 8_3:-25380768 None:intergenic
TCTACAGCCATCATGGTTGC+AGG 0.425416 8_3:-25380614 None:intergenic
GGACACGGCAGAACCTTGTC+TGG 0.427129 8_3:-25380065 None:intergenic
AGAAACTGTGTTAAATATCA+AGG 0.432446 8_3:+25380337 Msa1283750:CDS
GTTTCTACCGATTCACTAAG+AGG 0.436362 8_3:-25380320 None:intergenic
GTGTCCTCGCTGCAATAGTT+TGG 0.447623 8_3:+25380082 Msa1283750:CDS
CACGCTTTCCTGAAAGGATC+CGG 0.450006 8_3:-25381050 None:intergenic
GACTTATCCCTGTTGGAATC+CGG 0.450042 8_3:+25380559 Msa1283750:CDS
CATTCCATCACCTTCCTCTT+CGG 0.453115 8_3:+25380727 Msa1283750:CDS
CTGTTCAGATTTCGTTCTCA+AGG 0.456636 8_3:-25380909 None:intergenic
CTGTGATAGCTGCTGAATCC+GGG 0.458061 8_3:+25380426 Msa1283750:CDS
TTCTGAATGCTCCATTTCCC+CGG 0.459948 8_3:-25380444 None:intergenic
CAATACCGGTACTTTGAAAC+CGG 0.464327 8_3:+25379962 Msa1283750:CDS
CTCCATGGACTTATCCCTGT+TGG 0.466430 8_3:+25380552 Msa1283750:CDS
CCGGAGCGAGAGAAAGCAGC+AGG 0.481752 8_3:-25381031 None:intergenic
TTAGGAAAACATTGTAGAGA+TGG 0.504456 8_3:+25380788 Msa1283750:CDS
CGGTACTTCTACAGCCATCA+TGG 0.505901 8_3:-25380621 None:intergenic
GGAAGTTCCTCTTAGTGAAT+CGG 0.506714 8_3:+25380313 Msa1283750:CDS
TGTCAGAGGTATTGGACAGC+TGG 0.509122 8_3:+25380167 Msa1283750:CDS
AGGCTGCTAAGAGTTCTATA+TGG 0.510382 8_3:+25380882 Msa1283750:CDS
ACTAAGAGGAACTTCCTCCT+TGG 0.511294 8_3:-25380306 None:intergenic
ATTATTACTCCGCTCCTCCA+TGG 0.512821 8_3:+25380537 Msa1283750:CDS
CCTGCTGCTTTCTCTCGCTC+CGG 0.517821 8_3:+25381031 Msa1283750:CDS
GGCTGTATGCCAAATTGGAT+TGG 0.523809 8_3:+25380662 Msa1283750:CDS
TGTCACCGGTTTCAAAGTAC+CGG 0.526533 8_3:-25379967 None:intergenic
AGGAATGTTCCTATTGGAGC+CGG 0.533831 8_3:+25380200 Msa1283750:CDS
ATATTACACGCTTTCCTGAA+AGG 0.538533 8_3:-25381056 None:intergenic
GAAATGGAGCATTCAGAAAG+TGG 0.543981 8_3:+25380449 Msa1283750:CDS
TTAGTTGGATCTGCGTTCAA+CGG 0.546446 8_3:+25380704 Msa1283750:CDS
TTGGAATCTATGTGTATGAC+AGG 0.546648 8_3:-25380287 None:intergenic
AATGAAAAGCATGACCATGA+TGG 0.548748 8_3:-25378741 None:intergenic
ATTCCAAACATGTACAATAC+CGG 0.552268 8_3:+25379948 Msa1283750:CDS
TAATAATGAAGCGAATGTAA+AGG 0.553509 8_3:-25380521 None:intergenic
CAGAATTAATGATCCAGAAG+AGG 0.559620 8_3:+25380862 Msa1283750:CDS
TTATCAGGCTTAAATGCCAC+CGG 0.562271 8_3:-25380578 None:intergenic
CAGAGGTATTGGACAGCTGG+TGG 0.563331 8_3:+25380170 Msa1283750:CDS
TGTCATACACATAGATTCCA+AGG 0.569094 8_3:+25380289 Msa1283750:CDS
TATGACAGGTACTGCATCAA+GGG 0.571285 8_3:-25380273 None:intergenic
CAGAACAAACCTATTATGAA+AGG 0.575243 8_3:+25380929 Msa1283750:CDS
CCCCAATACGAAGGTGTCAT+CGG 0.575911 8_3:-25380641 None:intergenic
GTCAAGTCCTGCAACCATGA+TGG 0.576641 8_3:+25380607 Msa1283750:CDS
AAACTATTGCAGCGAGGACA+CGG 0.577128 8_3:-25380080 None:intergenic
TTCCAACAGGGATAAGTCCA+TGG 0.577632 8_3:-25380554 None:intergenic
ATAGTATTAGCATCTTCTTG+TGG 0.578337 8_3:-25379927 None:intergenic
GAAAGTGGAATAGAGCATGT+TGG 0.578755 8_3:+25380464 Msa1283750:CDS
AGCAGCTATCACAGAAGACC+TGG 0.580944 8_3:-25380417 None:intergenic
GAAACTGTGTTAAATATCAA+GGG 0.585315 8_3:+25380338 Msa1283750:CDS
GGAATTAAGCCGTTGCATTG+TGG 0.587832 8_3:-25380500 None:intergenic
CCGATGACACCTTCGTATTG+GGG 0.589925 8_3:+25380641 Msa1283750:CDS
TGTTGATGTTCGTACGAATG+AGG 0.589965 8_3:+25379884 Msa1283750:CDS
AGGATATTGAGAACTCGTCC+AGG 0.596188 8_3:+25380399 Msa1283750:CDS
GTATGACAGGTACTGCATCA+AGG 0.597457 8_3:-25380274 None:intergenic
TCCACATCAACAATCCATCA+TGG 0.598956 8_3:+25378727 Msa1283750:CDS
ACTTTGACACCACAATGCAA+CGG 0.601139 8_3:+25380491 Msa1283750:CDS
ACCATGATGGATTGTTGATG+TGG 0.602567 8_3:-25378728 None:intergenic
AGTACCACTGCTAGCCGAAG+AGG 0.608123 8_3:-25380741 None:intergenic
CTAGCCGAAGAGGAAGGTGA+TGG 0.608712 8_3:-25380731 None:intergenic
CCTTCCTCTTCGGCTAGCAG+TGG 0.612556 8_3:+25380737 Msa1283750:CDS
ATTACAATGTTAACCAACCA+AGG 0.617440 8_3:+25380129 Msa1283750:CDS
GGACGCTTCTACTGTCAAAG+AGG 0.623946 8_3:+25380379 Msa1283750:CDS
GTGAATCTGTGAATCTGTGG+TGG 0.627558 8_3:-25378699 None:intergenic
TTATCCCTGTTGGAATCCGG+TGG 0.630569 8_3:+25380562 Msa1283750:CDS
TTTGAAACCGGTGACAAAAG+CGG 0.633261 8_3:+25379974 Msa1283750:CDS
TGTGATAGCTGCTGAATCCG+GGG 0.635766 8_3:+25380427 Msa1283750:CDS
GGAACTTGACAATTCTGCAA+AGG 0.639283 8_3:-25380245 None:intergenic
GGCATACAGCCCCAATACGA+AGG 0.648370 8_3:-25380650 None:intergenic
GGGATAAGTCCATGGAGGAG+CGG 0.648425 8_3:-25380546 None:intergenic
CGGAGCGAGAGAAAGCAGCA+GGG 0.664686 8_3:-25381030 None:intergenic
TGAATCTGTGAATCTGTGGT+GGG 0.669351 8_3:-25378698 None:intergenic
AGAGTGAATCTGTGAATCTG+TGG 0.680020 8_3:-25378702 None:intergenic
AATGCCACCGGATTCCAACA+GGG 0.686661 8_3:-25380566 None:intergenic
CCACTGCTAGCCGAAGAGGA+AGG 0.690209 8_3:-25380737 None:intergenic
GTCTCCAAACTATTGCAGCG+AGG 0.702604 8_3:-25380086 None:intergenic
CAACAGGGATAAGTCCATGG+AGG 0.740228 8_3:-25380551 None:intergenic
CATACACATAGATTCCAAGG+AGG 0.765853 8_3:+25380292 Msa1283750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATAAGAAAGATAAAA+AGG - chr8_3:25378788-25378807 None:intergenic 10.0%
!!! TATCTTTCTTATATATTTTT+TGG + chr8_3:25378791-25378810 Msa1283750:intron 10.0%
!! AATATGAAAAATAAAATTCT+GGG - chr8_3:25378869-25378888 None:intergenic 10.0%
!!! ATTTTATTTTTCATATTGAA+TGG + chr8_3:25378872-25378891 Msa1283750:intron 10.0%
!! AAATTAAAATGAAATATGTT+AGG + chr8_3:25379394-25379413 Msa1283750:intron 10.0%
!! CAATATGAAAAATAAAATTC+TGG - chr8_3:25378870-25378889 None:intergenic 15.0%
!!! TGTTTGATAATTTATTTTGA+AGG + chr8_3:25379010-25379029 Msa1283750:intron 15.0%
!! TGACATGAAAAATATTAAAT+TGG + chr8_3:25379147-25379166 Msa1283750:intron 15.0%
!!! ATATTTTTCATGTCAATTAC+TGG - chr8_3:25379142-25379161 None:intergenic 20.0%
!! TAATCAAACAGTTAAAGAAT+AGG - chr8_3:25379491-25379510 None:intergenic 20.0%
!! AAATCAATGTTGTTAAATAG+TGG + chr8_3:25379586-25379605 Msa1283750:intron 20.0%
!!! TATTGACTTATTCAAATAAG+CGG - chr8_3:25379708-25379727 None:intergenic 20.0%
!!! TATCAATGAGTTATAACTTT+TGG + chr8_3:25379771-25379790 Msa1283750:intron 20.0%
!!! TCATTAATTCTGATAGTTTT+CGG - chr8_3:25380854-25380873 None:intergenic 20.0%
!! GTTGTTGTTCTTGTTTAATA+TGG - chr8_3:25378622-25378641 None:intergenic 25.0%
!!! ATTTTGATTTTGTTCTAGCT+AGG + chr8_3:25378977-25378996 Msa1283750:intron 25.0%
! GAATTATTTGTGTTTGAATC+TGG + chr8_3:25379089-25379108 Msa1283750:intron 25.0%
!!! TTGAATCTGGTTTTTTAAGT+TGG + chr8_3:25379102-25379121 Msa1283750:intron 25.0%
! AAATTGGACCAAATTAATTG+AGG + chr8_3:25379163-25379182 Msa1283750:intron 25.0%
! TGTTCTATTTACTGTATCAT+TGG + chr8_3:25379188-25379207 Msa1283750:intron 25.0%
!! TTAATTTTGTGTTAATCCTC+TGG - chr8_3:25379381-25379400 None:intergenic 25.0%
! TAAACAATACCAATAACTCA+TGG - chr8_3:25379530-25379549 None:intergenic 25.0%
! TATTGGTATTGTTTAATCTG+TGG + chr8_3:25379535-25379554 Msa1283750:intron 25.0%
! ATTGGTATTGTTTAATCTGT+GGG + chr8_3:25379536-25379555 Msa1283750:intron 25.0%
! TTGTACTAAATAATGTATCG+TGG - chr8_3:25379649-25379668 None:intergenic 25.0%
!! TTGATAGTATGCATTTCTAT+CGG - chr8_3:25379757-25379776 None:intergenic 25.0%
! TTAAAAAGACAACTTGCATT+CGG - chr8_3:25379798-25379817 None:intergenic 25.0%
! AGAAACTGTGTTAAATATCA+AGG + chr8_3:25380337-25380356 Msa1283750:CDS 25.0%
! GAAACTGTGTTAAATATCAA+GGG + chr8_3:25380338-25380357 Msa1283750:CDS 25.0%
! TAATAATGAAGCGAATGTAA+AGG - chr8_3:25380524-25380543 None:intergenic 25.0%
! AAAACACTTCCTTTCATAAT+AGG - chr8_3:25380941-25380960 None:intergenic 25.0%
! GTGTTACTACAGAAAATAAG+TGG + chr8_3:25379053-25379072 Msa1283750:intron 30.0%
TACTACAGAAAATAAGTGGT+AGG + chr8_3:25379057-25379076 Msa1283750:intron 30.0%
ACTACAGAAAATAAGTGGTA+GGG + chr8_3:25379058-25379077 Msa1283750:intron 30.0%
AGAACACACCTCAATTAATT+TGG - chr8_3:25379174-25379193 None:intergenic 30.0%
ATAATTTCAGCCCAATTACT+CGG - chr8_3:25379229-25379248 None:intergenic 30.0%
ACTAAGTATAAATCGTTCGT+GGG + chr8_3:25379260-25379279 Msa1283750:intron 30.0%
TTTGACCTGTTGTTATGTTA+AGG + chr8_3:25379429-25379448 Msa1283750:intron 30.0%
TTTGACCTTAACATAACAAC+AGG - chr8_3:25379437-25379456 None:intergenic 30.0%
AGAGTAAAACCATGAGTTAT+TGG + chr8_3:25379518-25379537 Msa1283750:intron 30.0%
ATAGTATTAGCATCTTCTTG+TGG - chr8_3:25379930-25379949 None:intergenic 30.0%
ATTCCAAACATGTACAATAC+CGG + chr8_3:25379948-25379967 Msa1283750:CDS 30.0%
ATTACAATGTTAACCAACCA+AGG + chr8_3:25380129-25380148 Msa1283750:CDS 30.0%
ATTTCTGCAAAAATTGTCAG+AGG + chr8_3:25380153-25380172 Msa1283750:CDS 30.0%
GTAATTAGGAATGTTCCTAT+TGG + chr8_3:25380194-25380213 Msa1283750:CDS 30.0%
! CGTCCATTTCAATTTTTCTA+TGG - chr8_3:25380364-25380383 None:intergenic 30.0%
CTAACGATAATTCTTCCATT+TGG - chr8_3:25380688-25380707 None:intergenic 30.0%
ATGGAAGAATTATCGTTAGT+TGG + chr8_3:25380689-25380708 Msa1283750:CDS 30.0%
! CTACAATGTTTTCCTAAAGT+TGG - chr8_3:25380785-25380804 None:intergenic 30.0%
TTAGGAAAACATTGTAGAGA+TGG + chr8_3:25380788-25380807 Msa1283750:CDS 30.0%
CAGAACAAACCTATTATGAA+AGG + chr8_3:25380929-25380948 Msa1283750:CDS 30.0%
! GATCCTTCAATCAAGCTTTT+TGG + chr8_3:25378653-25378672 Msa1283750:CDS 35.0%
CTTCCAAAAAGCTTGATTGA+AGG - chr8_3:25378659-25378678 None:intergenic 35.0%
! AATGAAAAGCATGACCATGA+TGG - chr8_3:25378744-25378763 None:intergenic 35.0%
AAGGTGCAAAAAAGATGCAA+AGG - chr8_3:25378769-25378788 None:intergenic 35.0%
CTGTATCATTGGAATATGTG+TGG + chr8_3:25379199-25379218 Msa1283750:intron 35.0%
CACGAACGATTTATACTTAG+TGG - chr8_3:25379261-25379280 None:intergenic 35.0%
CACTAAGTATAAATCGTTCG+TGG + chr8_3:25379259-25379278 Msa1283750:intron 35.0%
CAAATAGCAGTGATATAGCA+CGG + chr8_3:25379677-25379696 Msa1283750:intron 35.0%
TTTGGTACTCAATTGCAGAA+TGG + chr8_3:25379837-25379856 Msa1283750:intron 35.0%
! TTGATTGTCAGCATCTCTTT+TGG - chr8_3:25380027-25380046 None:intergenic 35.0%
! CAATTTTTGCAGAAATGCCT+TGG - chr8_3:25380149-25380168 None:intergenic 35.0%
! GCAAAAATTGTCAGAGGTAT+TGG + chr8_3:25380159-25380178 Msa1283750:CDS 35.0%
!! TTGCTTATTTCTACGCTTAC+CGG - chr8_3:25380222-25380241 None:intergenic 35.0%
TTGGAATCTATGTGTATGAC+AGG - chr8_3:25380290-25380309 None:intergenic 35.0%
TGTCATACACATAGATTCCA+AGG + chr8_3:25380289-25380308 Msa1283750:CDS 35.0%
GGGCCATAGAAAAATTGAAA+TGG + chr8_3:25380358-25380377 Msa1283750:CDS 35.0%
!! TAAAGTTGGTGACCTATTAC+CGG - chr8_3:25380771-25380790 None:intergenic 35.0%
ATACGATGAAGCCGAATATT+TGG + chr8_3:25380825-25380844 Msa1283750:CDS 35.0%
TACGATGAAGCCGAATATTT+GGG + chr8_3:25380826-25380845 Msa1283750:CDS 35.0%
CAGAATTAATGATCCAGAAG+AGG + chr8_3:25380862-25380881 Msa1283750:CDS 35.0%
TGAATCTGTGAATCTGTGGT+GGG - chr8_3:25378701-25378720 None:intergenic 40.0%
AGAGTGAATCTGTGAATCTG+TGG - chr8_3:25378705-25378724 None:intergenic 40.0%
TCCACATCAACAATCCATCA+TGG + chr8_3:25378727-25378746 Msa1283750:CDS 40.0%
!! ACCATGATGGATTGTTGATG+TGG - chr8_3:25378731-25378750 None:intergenic 40.0%
TTCCTGAGTGGGATAATTCT+TGG + chr8_3:25379296-25379315 Msa1283750:intron 40.0%
GTCAGACTTTACTTACGTTC+TGG + chr8_3:25379318-25379337 Msa1283750:intron 40.0%
! GTTAATCCTCTGGTTCCTAA+GGG - chr8_3:25379371-25379390 None:intergenic 40.0%
! TGTTAATCCTCTGGTTCCTA+AGG - chr8_3:25379372-25379391 None:intergenic 40.0%
GTATGTCAACTGACACAGTT+TGG + chr8_3:25379819-25379838 Msa1283750:intron 40.0%
TGTTGATGTTCGTACGAATG+AGG + chr8_3:25379884-25379903 Msa1283750:CDS 40.0%
!! TTGTGGATTAGCTTGTTTGC+TGG - chr8_3:25379913-25379932 None:intergenic 40.0%
! GTACCGGTATTGTACATGTT+TGG - chr8_3:25379954-25379973 None:intergenic 40.0%
!! CAATACCGGTACTTTGAAAC+CGG + chr8_3:25379962-25379981 Msa1283750:CDS 40.0%
! TTTGAAACCGGTGACAAAAG+CGG + chr8_3:25379974-25379993 Msa1283750:CDS 40.0%
!! AGTTTTCAAGAAGCCAGACA+AGG + chr8_3:25380052-25380071 Msa1283750:CDS 40.0%
! TTTTGCAGAAATGCCTTGGT+TGG - chr8_3:25380145-25380164 None:intergenic 40.0%
GGAACTTGACAATTCTGCAA+AGG - chr8_3:25380248-25380267 None:intergenic 40.0%
! TATGACAGGTACTGCATCAA+GGG - chr8_3:25380276-25380295 None:intergenic 40.0%
CATACACATAGATTCCAAGG+AGG + chr8_3:25380292-25380311 Msa1283750:CDS 40.0%
GGAAGTTCCTCTTAGTGAAT+CGG + chr8_3:25380313-25380332 Msa1283750:CDS 40.0%
!! GTTTCTACCGATTCACTAAG+AGG - chr8_3:25380323-25380342 None:intergenic 40.0%
! GAAATGGAGCATTCAGAAAG+TGG + chr8_3:25380449-25380468 Msa1283750:CDS 40.0%
GAAAGTGGAATAGAGCATGT+TGG + chr8_3:25380464-25380483 Msa1283750:CDS 40.0%
ACTTTGACACCACAATGCAA+CGG + chr8_3:25380491-25380510 Msa1283750:CDS 40.0%
TTATCAGGCTTAAATGCCAC+CGG - chr8_3:25380581-25380600 None:intergenic 40.0%
GGACTTGACGTTATGTTATC+AGG - chr8_3:25380596-25380615 None:intergenic 40.0%
TCCATTTGGCCAATCCAATT+TGG - chr8_3:25380674-25380693 None:intergenic 40.0%
TTAGTTGGATCTGCGTTCAA+CGG + chr8_3:25380704-25380723 Msa1283750:CDS 40.0%
GGTAATAGGTCACCAACTTT+AGG + chr8_3:25380770-25380789 Msa1283750:CDS 40.0%
! GTTTTCGGCACCCAAATATT+CGG - chr8_3:25380839-25380858 None:intergenic 40.0%
AGGCTGCTAAGAGTTCTATA+TGG + chr8_3:25380882-25380901 Msa1283750:CDS 40.0%
CTGTTCAGATTTCGTTCTCA+AGG - chr8_3:25380912-25380931 None:intergenic 40.0%
! CGAGAACTTGCATTTGACTT+AGG - chr8_3:25380977-25380996 None:intergenic 40.0%
GTGAATCTGTGAATCTGTGG+TGG - chr8_3:25378702-25378721 None:intergenic 45.0%
TGTGTGGTTGACCGAGTAAT+TGG + chr8_3:25379215-25379234 Msa1283750:intron 45.0%
GTGTGGTTGACCGAGTAATT+GGG + chr8_3:25379216-25379235 Msa1283750:intron 45.0%
ATCTGAGTTCGATTCCTGAG+TGG + chr8_3:25379284-25379303 Msa1283750:intron 45.0%
TCTGAGTTCGATTCCTGAGT+GGG + chr8_3:25379285-25379304 Msa1283750:intron 45.0%
GACCAAGAATTATCCCACTC+AGG - chr8_3:25379301-25379320 None:intergenic 45.0%
!! TCTATCGGTGCTGTCAATCA+CGG - chr8_3:25379742-25379761 None:intergenic 45.0%
!! TGTCACCGGTTTCAAAGTAC+CGG - chr8_3:25379970-25379989 None:intergenic 45.0%
!! CTATGAACCGCTTTTGTCAC+CGG - chr8_3:25379984-25380003 None:intergenic 45.0%
AAACTATTGCAGCGAGGACA+CGG - chr8_3:25380083-25380102 None:intergenic 45.0%
AGGAATGTTCCTATTGGAGC+CGG + chr8_3:25380200-25380219 Msa1283750:CDS 45.0%
! GTATGACAGGTACTGCATCA+AGG - chr8_3:25380277-25380296 None:intergenic 45.0%
ACTAAGAGGAACTTCCTCCT+TGG - chr8_3:25380309-25380328 None:intergenic 45.0%
AGGATATTGAGAACTCGTCC+AGG + chr8_3:25380399-25380418 Msa1283750:CDS 45.0%
TCTGTGATAGCTGCTGAATC+CGG + chr8_3:25380425-25380444 Msa1283750:CDS 45.0%
TTCTGAATGCTCCATTTCCC+CGG - chr8_3:25380447-25380466 None:intergenic 45.0%
GGAATTAAGCCGTTGCATTG+TGG - chr8_3:25380503-25380522 None:intergenic 45.0%
ATTATTACTCCGCTCCTCCA+TGG + chr8_3:25380537-25380556 Msa1283750:CDS 45.0%
TTCCAACAGGGATAAGTCCA+TGG - chr8_3:25380557-25380576 None:intergenic 45.0%
GACTTATCCCTGTTGGAATC+CGG + chr8_3:25380559-25380578 Msa1283750:CDS 45.0%
TACCGATGACACCTTCGTAT+TGG + chr8_3:25380639-25380658 Msa1283750:CDS 45.0%
ACCGATGACACCTTCGTATT+GGG + chr8_3:25380640-25380659 Msa1283750:CDS 45.0%
ATTGGGGCTGTATGCCAAAT+TGG + chr8_3:25380657-25380676 Msa1283750:CDS 45.0%
GGCTGTATGCCAAATTGGAT+TGG + chr8_3:25380662-25380681 Msa1283750:CDS 45.0%
!! GCCAAATTGGATTGGCCAAA+TGG + chr8_3:25380670-25380689 Msa1283750:CDS 45.0%
CATTCCATCACCTTCCTCTT+CGG + chr8_3:25380727-25380746 Msa1283750:CDS 45.0%
GTGGTACTTGTTCCGGTAAT+AGG + chr8_3:25380756-25380775 Msa1283750:CDS 45.0%
CGTTCCAGTAATCCGGAGTT+CGG - chr8_3:25379344-25379363 None:intergenic 50.0%
TTACTGGAACGCCTTCCCTT+AGG + chr8_3:25379353-25379372 Msa1283750:intron 50.0%
! ATCCTCTGGTTCCTAAGGGA+AGG - chr8_3:25379367-25379386 None:intergenic 50.0%
GTGTCCTCGCTGCAATAGTT+TGG + chr8_3:25380082-25380101 Msa1283750:CDS 50.0%
GTCTCCAAACTATTGCAGCG+AGG - chr8_3:25380089-25380108 None:intergenic 50.0%
!! TGTCAGAGGTATTGGACAGC+TGG + chr8_3:25380167-25380186 Msa1283750:CDS 50.0%
GGACAGCTGGTGGAGTAATT+AGG + chr8_3:25380180-25380199 Msa1283750:CDS 50.0%
GGTACTGCATCAAGGGTAAC+AGG - chr8_3:25380269-25380288 None:intergenic 50.0%
GGACGCTTCTACTGTCAAAG+AGG + chr8_3:25380379-25380398 Msa1283750:CDS 50.0%
AGCAGCTATCACAGAAGACC+TGG - chr8_3:25380420-25380439 None:intergenic 50.0%
CTGTGATAGCTGCTGAATCC+GGG + chr8_3:25380426-25380445 Msa1283750:CDS 50.0%
TGTGATAGCTGCTGAATCCG+GGG + chr8_3:25380427-25380446 Msa1283750:CDS 50.0%
CAACAGGGATAAGTCCATGG+AGG - chr8_3:25380554-25380573 None:intergenic 50.0%
CTCCATGGACTTATCCCTGT+TGG + chr8_3:25380552-25380571 Msa1283750:CDS 50.0%
TTATCCCTGTTGGAATCCGG+TGG + chr8_3:25380562-25380581 Msa1283750:CDS 50.0%
AATGCCACCGGATTCCAACA+GGG - chr8_3:25380569-25380588 None:intergenic 50.0%
AAATGCCACCGGATTCCAAC+AGG - chr8_3:25380570-25380589 None:intergenic 50.0%
GTCAAGTCCTGCAACCATGA+TGG + chr8_3:25380607-25380626 Msa1283750:CDS 50.0%
TCTACAGCCATCATGGTTGC+AGG - chr8_3:25380617-25380636 None:intergenic 50.0%
CGGTACTTCTACAGCCATCA+TGG - chr8_3:25380624-25380643 None:intergenic 50.0%
!! CCCCAATACGAAGGTGTCAT+CGG - chr8_3:25380644-25380663 None:intergenic 50.0%
CCGATGACACCTTCGTATTG+GGG + chr8_3:25380641-25380660 Msa1283750:CDS 50.0%
GCTAGCAGTGGTACTTGTTC+CGG + chr8_3:25380749-25380768 Msa1283750:CDS 50.0%
GAACTCTTAGCAGCCTCTTC+TGG - chr8_3:25380878-25380897 None:intergenic 50.0%
CACGCTTTCCTGAAAGGATC+CGG - chr8_3:25381053-25381072 None:intergenic 50.0%
CTTACGTTCTGGCCGAACTC+CGG + chr8_3:25379329-25379348 Msa1283750:intron 55.0%
!! GGGAAGGCGTTCCAGTAATC+CGG - chr8_3:25379351-25379370 None:intergenic 55.0%
!! CAGAGGTATTGGACAGCTGG+TGG + chr8_3:25380170-25380189 Msa1283750:CDS 55.0%
!! CTACGCTTACCGGCTCCAAT+AGG - chr8_3:25380212-25380231 None:intergenic 55.0%
AGCTGCTGAATCCGGGGAAA+TGG + chr8_3:25380433-25380452 Msa1283750:CDS 55.0%
GGGATAAGTCCATGGAGGAG+CGG - chr8_3:25380549-25380568 None:intergenic 55.0%
GGCATACAGCCCCAATACGA+AGG - chr8_3:25380653-25380672 None:intergenic 55.0%
CTAGCCGAAGAGGAAGGTGA+TGG - chr8_3:25380734-25380753 None:intergenic 55.0%
AGTACCACTGCTAGCCGAAG+AGG - chr8_3:25380744-25380763 None:intergenic 55.0%
CTGGCCGAACTCCGGATTAC+TGG + chr8_3:25379337-25379356 Msa1283750:intron 60.0%
CGCCTTCCCTTAGGAACCAG+AGG + chr8_3:25379362-25379381 Msa1283750:intron 60.0%
GGACACGGCAGAACCTTGTC+TGG - chr8_3:25380068-25380087 None:intergenic 60.0%
CCACTGCTAGCCGAAGAGGA+AGG - chr8_3:25380740-25380759 None:intergenic 60.0%
CCTTCCTCTTCGGCTAGCAG+TGG + chr8_3:25380737-25380756 Msa1283750:CDS 60.0%
CGGAGCGAGAGAAAGCAGCA+GGG - chr8_3:25381033-25381052 None:intergenic 60.0%
!! CCTGCTGCTTTCTCTCGCTC+CGG + chr8_3:25381031-25381050 Msa1283750:CDS 60.0%
CTCTCGCTCCGGATCCTTTC+AGG + chr8_3:25381042-25381061 Msa1283750:CDS 60.0%
CCGGAGCGAGAGAAAGCAGC+AGG - chr8_3:25381034-25381053 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 25378614 25381075 25378614 ID=Msa1283750;Name=Msa1283750
chr8_3 mRNA 25378614 25381075 25378614 ID=Msa1283750-mRNA-1;Parent=Msa1283750;Name=Msa1283750-mRNA-1;_AED=0.01;_eAED=0.03;_QI=0|1|0|1|1|1|2|0|451
chr8_3 exon 25378614 25378748 25378614 ID=Msa1283750-mRNA-1:exon:8882;Parent=Msa1283750-mRNA-1
chr8_3 exon 25379855 25381075 25379855 ID=Msa1283750-mRNA-1:exon:8883;Parent=Msa1283750-mRNA-1
chr8_3 CDS 25378614 25378748 25378614 ID=Msa1283750-mRNA-1:cds;Parent=Msa1283750-mRNA-1
chr8_3 CDS 25379855 25381075 25379855 ID=Msa1283750-mRNA-1:cds;Parent=Msa1283750-mRNA-1
Gene Sequence

>Msa1283750

ATGTTCCATATTAAACAAGAACAACAACAACAACAAGAAGATCCTTCAATCAAGCTTTTTGGAAGTGATATTCAAATTCATACTCCCACCACAGATTCACAGATTCACTCTCTTCCACATCAACAATCCATCATGAATGGACTTGAAGAATCAGCTAGTGCTGATGTTGATGTTCGTACGAATGAGGTTTCCAGCAAACAAGCTAATCCACAAGAAGATGCTAATACTATTCCAAACATGTACAATACCGGTACTTTGAAACCGGTGACAAAAGCGGTTCATAGAACTAATGAACACAAGACTGCCAAAAGAGATGCTGACAATCAAGAGAAAGTTTTCAAGAAGCCAGACAAGGTTCTGCCGTGTCCTCGCTGCAATAGTTTGGAGACAAAGTTTTGCTATTTCAACAATTACAATGTTAACCAACCAAGGCATTTCTGCAAAAATTGTCAGAGGTATTGGACAGCTGGTGGAGTAATTAGGAATGTTCCTATTGGAGCCGGTAAGCGTAGAAATAAGCAATCGCCTTTGCAGAATTGTCAAGTTCCTGTTACCCTTGATGCAGTACCTGTCATACACATAGATTCCAAGGAGGAAGTTCCTCTTAGTGAATCGGTAGAAACTGTGTTAAATATCAAGGGCCATAGAAAAATTGAAATGGACGCTTCTACTGTCAAAGAGGATATTGAGAACTCGTCCAGGTCTTCTGTGATAGCTGCTGAATCCGGGGAAATGGAGCATTCAGAAAGTGGAATAGAGCATGTTGGTTTGACTTTGACACCACAATGCAACGGCTTAATTCCTTTACATTCGCTTCATTATTACTCCGCTCCTCCATGGACTTATCCCTGTTGGAATCCGGTGGCATTTAAGCCTGATAACATAACGTCAAGTCCTGCAACCATGATGGCTGTAGAAGTACCGATGACACCTTCGTATTGGGGCTGTATGCCAAATTGGATTGGCCAAATGGAAGAATTATCGTTAGTTGGATCTGCGTTCAACGGCATTCCATCACCTTCCTCTTCGGCTAGCAGTGGTACTTGTTCCGGTAATAGGTCACCAACTTTAGGAAAACATTGTAGAGATGGAAGTACACAAGAAGATACGATGAAGCCGAATATTTGGGTGCCGAAAACTATCAGAATTAATGATCCAGAAGAGGCTGCTAAGAGTTCTATATGGTCAACCTTGAGAACGAAATCTGAACAGAACAAACCTATTATGAAAGGAAGTGTTTTTAAATCATTTGAACCTAAGTCAAATGCAAGTTCTCGTGATTTAGACGATAATCAAATTCTACGAGCTAACCCTGCTGCTTTCTCTCGCTCCGGATCCTTTCAGGAAAGCGTGTAA
Protein sequence

>Msa1283750

MFHIKQEQQQQQEDPSIKLFGSDIQIHTPTTDSQIHSLPHQQSIMNGLEESASADVDVRTNEVSSKQANPQEDANTIPNMYNTGTLKPVTKAVHRTNEHKTAKRDADNQEKVFKKPDKVLPCPRCNSLETKFCYFNNYNVNQPRHFCKNCQRYWTAGGVIRNVPIGAGKRRNKQSPLQNCQVPVTLDAVPVIHIDSKEEVPLSESVETVLNIKGHRKIEMDASTVKEDIENSSRSSVIAAESGEMEHSESGIEHVGLTLTPQCNGLIPLHSLHYYSAPPWTYPCWNPVAFKPDNITSSPATMMAVEVPMTPSYWGCMPNWIGQMEELSLVGSAFNGIPSPSSSASSGTCSGNRSPTLGKHCRDGSTQEDTMKPNIWVPKTIRINDPEEAAKSSIWSTLRTKSEQNKPIMKGSVFKSFEPKSNASSRDLDDNQILRANPAAFSRSGSFQESV*