AlfalfaGEDB | Msa1285240

M. sativa cultivar zhongmu-4 / Msa1285240

KEGG: K02206
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1285240	sp\|Q41639\|CDC2_VIGAC	48.299	294	147	2	48	341	2	290	9.58e-92	288

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1285240	A0A396GIJ5	92.420	343	23	1	1	343	1	340	0.0	672

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1285240	MtrunA17_Chr8g0358921	92.420	343	23	1	1	343	1	340	0.0	672
Msa1285240	MtrunA17_Chr4g0051261	47.279	294	150	2	48	341	2	290	5.18e-90	281
Msa1285240	MtrunA17_Chr2g0293491	46.259	294	153	2	48	341	2	290	2.39e-89	279
Msa1285240	MtrunA17_Chr8g0364621	75.132	189	44	1	446	634	3	188	6.73e-89	274
Msa1285240	MtrunA17_Chr1g0187371	42.809	299	165	4	48	342	16	312	4.05e-79	253
Msa1285240	MtrunA17_Chr4g0002001	41.801	311	162	7	47	342	1	307	1.01e-74	241
Msa1285240	MtrunA17_Chr3g0099951	40.278	288	160	6	54	338	18	296	1.92e-63	215
Msa1285240	MtrunA17_Chr6g0483151	39.860	286	162	5	54	338	18	294	1.10e-61	210
Msa1285240	MtrunA17_Chr3g0093391	34.121	381	215	11	53	406	26	397	1.71e-56	199
Msa1285240	MtrunA17_Chr3g0093571	38.235	306	160	8	53	338	28	324	1.73e-56	199

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1285240	AT3G48750.1	45.734	293	152	3	53	345	7	292	2.12e-87	275
Msa1285240	AT1G76540.1	43.686	293	159	4	53	341	17	307	2.09e-79	254
Msa1285240	AT1G20930.1	42.809	299	165	4	47	341	13	309	5.03e-78	251
Msa1285240	AT2G38620.2	41.424	309	166	6	47	342	1	307	7.93e-73	237
Msa1285240	AT3G54180.1	38.562	306	177	5	47	342	1	305	1.12e-67	223
Msa1285240	AT1G18040.1	39.510	286	163	5	54	338	16	292	1.43e-63	215
Msa1285240	AT1G73690.1	38.811	286	165	5	54	338	15	291	2.42e-62	212
Msa1285240	AT1G66750.1	39.437	284	162	5	56	338	19	293	6.20e-62	209
Msa1285240	AT1G24050.1	54.595	185	82	1	446	628	1	185	5.93e-61	201
Msa1285240	AT2G38620.4	37.162	296	135	4	47	342	1	245	1.28e-58	197

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Find 142 sgRNAs with CRISPR-Local

Find 307 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGAGACTTCTCTGATGA+TGG	0.476409	8_3:-29044525	None:intergenic
AAACGCTTCGGAGTTATTGT+TGG	0.483935	8_3:-29048398	None:intergenic
AAAGTTTGGGATGACGGTGG+AGG	0.569321	8_3:+29048303	Msa1285240:CDS
AACATCCTCGGACTCATGGA+AGG	0.581002	8_3:+29048502	Msa1285240:CDS
AACTCCGAAGCGTTTGTAGT+GGG	0.600740	8_3:+29048406	Msa1285240:CDS
AACTGGTCTTAATCTTCTAA+TGG	0.406752	8_3:+29046159	Msa1285240:CDS
AAGCAGAGGCAGATGCTGAG+AGG	0.662063	8_3:+29048680	Msa1285240:CDS
AAGCTTGGTGAAGGTGGTTT+CGG	0.390300	8_3:+29044410	Msa1285240:CDS
AATAACAATGCAGAAAAGTT+TGG	0.282433	8_3:+29048289	Msa1285240:CDS
AATAATTACCTAACAATGTT+AGG	0.349233	8_3:-29044566	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GACGAAAAAGAATTCAATGA+AGG	+	chr8_3:29044356-29044375	Msa1285240:CDS	30.0%
TGAATCATCCTAACATTGTT+AGG	+	chr8_3:29044558-29044577	Msa1285240:CDS	30.0%
GTTGAATGTAAAGTACAAGA+AGG	+	chr8_3:29044674-29044693	Msa1285240:CDS	30.0%
TAACGATCCAATGACTAAAA+AGG	+	chr8_3:29044785-29044804	Msa1285240:CDS	30.0%
TTTATCATGTTAGACAGTTG+TGG	+	chr8_3:29045221-29045240	Msa1285240:intron	30.0%
TAACTAGTAGAGTAACTGAA+AGG	+	chr8_3:29045453-29045472	Msa1285240:intron	30.0%
AACTAGTAGAGTAACTGAAA+GGG	+	chr8_3:29045454-29045473	Msa1285240:intron	30.0%
GAAATAGTTATTCACAAGAG+TGG	+	chr8_3:29045486-29045505	Msa1285240:intron	30.0%
TAATTTCACTCACACAAAAC+AGG	+	chr8_3:29045857-29045876	Msa1285240:intron	30.0%
ATTAGAAGATTAAGACCAGT+TGG	-	chr8_3:29046160-29046179	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	29044160	29048933	29044160	ID=Msa1285240;Name=Msa1285240
chr8_3	mRNA	29044160	29048933	29044160	ID=Msa1285240-mRNA-1;Parent=Msa1285240;Name=Msa1285240-mRNA-1;_AED=0.05;_eAED=0.08;_QI=0\|0\|0\|1\|1\|1\|9\|0\|634
chr8_3	exon	29044160	29044206	29044160	ID=Msa1285240-mRNA-1:exon:9698;Parent=Msa1285240-mRNA-1
chr8_3	exon	29044295	29044579	29044295	ID=Msa1285240-mRNA-1:exon:9699;Parent=Msa1285240-mRNA-1
chr8_3	exon	29044671	29044806	29044671	ID=Msa1285240-mRNA-1:exon:9700;Parent=Msa1285240-mRNA-1
chr8_3	exon	29044890	29045063	29044890	ID=Msa1285240-mRNA-1:exon:9701;Parent=Msa1285240-mRNA-1
chr8_3	exon	29045692	29045855	29045692	ID=Msa1285240-mRNA-1:exon:9702;Parent=Msa1285240-mRNA-1
chr8_3	exon	29045939	29046026	29045939	ID=Msa1285240-mRNA-1:exon:9703;Parent=Msa1285240-mRNA-1
chr8_3	exon	29046127	29046180	29046127	ID=Msa1285240-mRNA-1:exon:9704;Parent=Msa1285240-mRNA-1
chr8_3	exon	29046919	29046999	29046919	ID=Msa1285240-mRNA-1:exon:9705;Parent=Msa1285240-mRNA-1
chr8_3	exon	29048058	29048933	29048058	ID=Msa1285240-mRNA-1:exon:9706;Parent=Msa1285240-mRNA-1
chr8_3	CDS	29044160	29044206	29044160	ID=Msa1285240-mRNA-1:cds;Parent=Msa1285240-mRNA-1
chr8_3	CDS	29044295	29044579	29044295	ID=Msa1285240-mRNA-1:cds;Parent=Msa1285240-mRNA-1
chr8_3	CDS	29044671	29044806	29044671	ID=Msa1285240-mRNA-1:cds;Parent=Msa1285240-mRNA-1
chr8_3	CDS	29044890	29045063	29044890	ID=Msa1285240-mRNA-1:cds;Parent=Msa1285240-mRNA-1
chr8_3	CDS	29045692	29045855	29045692	ID=Msa1285240-mRNA-1:cds;Parent=Msa1285240-mRNA-1
chr8_3	CDS	29045939	29046026	29045939	ID=Msa1285240-mRNA-1:cds;Parent=Msa1285240-mRNA-1
chr8_3	CDS	29046127	29046180	29046127	ID=Msa1285240-mRNA-1:cds;Parent=Msa1285240-mRNA-1
chr8_3	CDS	29046919	29046999	29046919	ID=Msa1285240-mRNA-1:cds;Parent=Msa1285240-mRNA-1
chr8_3	CDS	29048058	29048933	29048058	ID=Msa1285240-mRNA-1:cds;Parent=Msa1285240-mRNA-1

Gene Sequence

>Msa1285240

ATGCTGAGAAATCTCCAAGAACACGATTCAAGTTACTCTGATTCTCTGTTTGTTCGCATCATAGCATGCGAATTATGCTGCAGTGCTTGTAATTCAAGGCGCAAGAAAGACGAAAAAGAATTCAATGAAGGAAAACCGTTCGAAAATCTGGATTTTCTTGAGAAGCTTGGTGAAGGTGGTTTCGGTACAGTTTTCCGTTGCTGCGACGTCGAAACTAAACGAATCGTGGCTGTTAAACAAGTTTCTATTCATGAAACTTATAATGCTGTTCCTGGTTCCATCATCAGAGAAGTCTCGTTTCTTAGAGAATTGAATCATCCTAACATTGTTAGGTTGTTGAATGTAAAGTACAAGAAGGGAACAAGACTCGTGCACTTGGTTTTAGAGTATCTTGATTGTGATCTTCATGATTACATTAGTGACGCGGAACGCTTCGATTCATCTATTAACGATCCAATGACTAAAAAGAGTTTTCTGTATCAGATACTATCTGCGGTGGAGTACTGTCATTCTCGCAAGATTATTCATAGAGATTTGAAACCGAGTAACCTTTTAATTGATCATTCTGAAAAGATAATCAAGTTGGCTGATTTTGGCTTGGCTAGAGAGTTGGGAGATCCTGACGTGTTATATTCGCCGAAGGTAGCAACTCGGTTTTATAGGGCACCTGAGATATTGTTTGAATGCGGCCAATATTCAACTCCAATTGATATTTGGTCTGTCGGCTGTATTTTTGGTGAGATGGTACTTGGAATACCAATTCTCCGAGCAATTGATTGCAAGGATGAACTAGAGACGATTTTCAGAATGTTCGGTACCCCAACCGAGGAAACATGGCCAGGAGTCACTGCTTTATGTGCTAACCTTCAAACCTATCCCAAATTTAGACCGATGGACCTGTCATCAATTTTTCCTCCTTTTGATCCAACTGGTCTTAATCTTCTAATGAGGATGCTTTGCTTGGACCCAAACAGGAGGATTTCTGCCGAAGCTGCTCTTAGACATGCTTACTTTGATGACTTGGATCCGGGTGGCAACAAAAACATCACAGAAGAGTTTGTGACTACGATGGAAGGTGGTAGCTGCACCGCCAACAGCAAAAATGTAGAAGAGTTTGCGACTACGATGGAGGATGGCAGCACCACCCCCACCAACAACAATGCAGAAGCGTCTGCGATGATGTTGGAGGGTGGCAGCAGTACCAACAAGGCAGAAGAGATTGTGACGATAATGGATGGTGTCAGCATCCCCACCACTGACAATAACAATGCAGAAAAGTTTGGGATGACGGTGGAGGATGACACCACCAGTAGCAACAATGCAGAGGAGTTTTTGATGACGATGGAGGGTGGCAGCAGCACCACACCAACCAACAATAACTCCGAAGCGTTTGTAGTGGGATGTTTGCTGTCGATCAGGACAACATTTGGTGAAGAATTTGAAGCCCAAGTTGTTACGTTTGATCATTCCTCCAACATCCTCGGACTCATGGAAGGTTCCAAAGATGCCCCTCTTAGAAAAATTAGGCTTTTGAACGCTGATTACATCCAAGACTTCACCTTTCTAGGTCAATATGAAGACCCACTTGATCCGCGTAATTTCTACGTTGATCTCAATGATCTCCAAGCAAGAGAAGAACTTGCCATTAGACAAGCAGAGGCAGATGCTGAGAGGATTGGGGTTGGTGTTACAAGCGAAGCGCAGAGCATCTTTGATGCTTTATCTAAAACGCTTCCAGTCCACTGGGACAAGACTGTCATAGTTGTAATGAATGAGGTGCATATCAGCAGTCCTTATCACTCGGATTGTGTGATTGGCGGTGCTCCTGCTGCCAATGACCGGGTGAAGAAAGTGCTTGAGATGTTGAGGAAGCGTTTGCAACTTCCCTGTTCTGGTGCTCAGTAG

Protein sequence

>Msa1285240

MLRNLQEHDSSYSDSLFVRIIACELCCSACNSRRKKDEKEFNEGKPFENLDFLEKLGEGGFGTVFRCCDVETKRIVAVKQVSIHETYNAVPGSIIREVSFLRELNHPNIVRLLNVKYKKGTRLVHLVLEYLDCDLHDYISDAERFDSSINDPMTKKSFLYQILSAVEYCHSRKIIHRDLKPSNLLIDHSEKIIKLADFGLARELGDPDVLYSPKVATRFYRAPEILFECGQYSTPIDIWSVGCIFGEMVLGIPILRAIDCKDELETIFRMFGTPTEETWPGVTALCANLQTYPKFRPMDLSSIFPPFDPTGLNLLMRMLCLDPNRRISAEAALRHAYFDDLDPGGNKNITEEFVTTMEGGSCTANSKNVEEFATTMEDGSTTPTNNNAEASAMMLEGGSSTNKAEEIVTIMDGVSIPTTDNNNAEKFGMTVEDDTTSSNNAEEFLMTMEGGSSTTPTNNNSEAFVVGCLLSIRTTFGEEFEAQVVTFDHSSNILGLMEGSKDAPLRKIRLLNADYIQDFTFLGQYEDPLDPRNFYVDLNDLQAREELAIRQAEADAERIGVGVTSEAQSIFDALSKTLPVHWDKTVIVVMNEVHISSPYHSDCVIGGAPAANDRVKKVLEMLRKRLQLPCSGAQ*