AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1285950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1285950 MtrunA17_Chr2g0289121 58.854 192 17 3 10 140 637 827 1.96e-61 201
Msa1285950 MtrunA17_Chr2g0289031 84.270 89 14 0 47 135 308 396 3.78e-48 159
Msa1285950 MtrunA17_Chr4g0075261 75.789 95 23 0 44 138 776 870 2.81e-45 157
Msa1285950 MtrunA17_Chr2g0289091 51.266 158 13 4 1 97 266 420 9.36e-38 132
Msa1285950 MtrunA17_Chr1g0211711 69.663 89 27 0 44 132 960 1048 2.02e-37 134
Msa1285950 MtrunA17_Chr1g0211701 65.169 89 31 0 44 132 27 115 2.32e-37 125
Msa1285950 MtrunA17_Chr5g0426961 69.663 89 27 0 44 132 744 832 8.01e-37 133
Msa1285950 MtrunA17_Chr1g0183311 68.539 89 28 0 44 132 791 879 1.37e-36 132
Msa1285950 MtrunA17_Chr7g0275771 66.279 86 29 0 47 132 839 924 5.25e-35 127
Msa1285950 MtrunA17_Chr1g0147021 62.366 93 35 0 47 139 1610 1702 1.18e-34 127
Msa1285950 MtrunA17_Chr8g0361391 41.398 186 10 3 15 141 18 163 1.72e-32 112
Msa1285950 MtrunA17_Chr5g0420361 46.970 132 54 3 1 116 648 779 2.77e-30 114
Msa1285950 MtrunA17_Chr6g0472441 65.753 73 25 0 44 116 629 701 7.90e-28 107
Msa1285950 MtrunA17_Chr4g0048531 50.000 84 42 0 48 131 1192 1275 6.05e-26 102
Msa1285950 MtrunA17_Chr2g0281051 46.429 84 45 0 48 131 870 953 1.16e-24 98.2
Msa1285950 MtrunA17_Chr1g0192011 41.304 92 54 0 44 135 872 963 1.50e-22 92.4
Msa1285950 MtrunA17_Chr1g0211671 54.386 57 26 0 76 132 17 73 8.06e-16 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1285950 AT1G11950.1 71.910 89 25 0 47 135 783 871 1.08e-41 147
Msa1285950 AT1G11950.3 71.910 89 25 0 47 135 783 871 1.08e-41 147
Msa1285950 AT3G07610.1 65.556 90 31 0 44 133 803 892 8.23e-37 133
Msa1285950 AT3G07610.3 65.556 90 31 0 44 133 825 914 8.82e-37 133
Msa1285950 AT1G62310.1 81.159 69 13 0 47 115 809 877 3.77e-34 125
Msa1285950 AT4G00990.1 53.684 95 44 0 44 138 741 835 2.88e-32 120
Msa1285950 AT1G09060.2 43.820 89 50 0 47 135 805 893 7.91e-21 87.8
Msa1285950 AT1G09060.1 43.820 89 50 0 47 135 805 893 7.91e-21 87.8
Msa1285950 AT1G09060.4 43.820 89 50 0 47 135 819 907 8.32e-21 87.8
Msa1285950 AT1G09060.3 43.820 89 50 0 47 135 819 907 8.32e-21 87.8
Msa1285950 AT4G21430.1 36.957 92 58 0 48 139 828 919 2.15e-18 80.9

Find 28 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTGCCCTGTGATTCTT+AGG 0.277632 8_3:+32505995 None:intergenic
GTGATTAGAGGAACTTGATT+TGG 0.307949 8_3:+32507577 None:intergenic
TGATCAAGTTGTTCAAGATT+TGG 0.356114 8_3:-32505799 Msa1285950:CDS
AAGAAATGGAAGGGCCTTCA+TGG 0.363671 8_3:-32508777 None:intergenic
GATGTATCTTCTGTGATTAG+AGG 0.363682 8_3:+32507565 None:intergenic
GAGGGTCTTGTTCAACATAA+TGG 0.371403 8_3:-32507631 Msa1285950:CDS
AGCTTTCCTTTGATCTCAAA+AGG 0.429428 8_3:+32507604 None:intergenic
TCACCTTCAGATTTCGAACT+TGG 0.433332 8_3:+32506223 None:intergenic
ACTTGGTGAGGGCATCCAAC+AGG 0.485363 8_3:+32506240 None:intergenic
GTCGCCTTCCTAAGAATCAC+AGG 0.498370 8_3:-32506003 Msa1285950:CDS
TTGCCCTGTGATTCTTAGGA+AGG 0.529013 8_3:+32505999 None:intergenic
TTTCATTAGATGCTCATCAC+TGG 0.540922 8_3:-32505772 Msa1285950:CDS
CAATTACAGGTGTAGTACCT+TGG 0.542531 8_3:-32506299 Msa1285950:intron
CTTTGTATCACCAGAAAATG+TGG 0.555701 8_3:-32506056 Msa1285950:CDS
TTCAGATTTCGAACTTGGTG+AGG 0.558607 8_3:+32506228 None:intergenic
GGCAAGAGAAGACAAACTTA+AGG 0.580172 8_3:-32505981 Msa1285950:intron
TCAGATTTCGAACTTGGTGA+GGG 0.580542 8_3:+32506229 None:intergenic
CAATAACTGGTTCAGAAAGA+GGG 0.585313 8_3:-32507649 Msa1285950:intron
AGGGAAATCCAAGAAATGGA+AGG 0.594356 8_3:-32508787 None:intergenic
AGCTTCTGCTCAAAAGTCCA+AGG 0.599846 8_3:+32506282 None:intergenic
GAAGCAGTATTTATACCTGT+TGG 0.603645 8_3:-32506255 Msa1285950:CDS
GAAGATACATCAGAAACAGC+TGG 0.604910 8_3:-32507553 Msa1285950:intron
TCACCAAGTTCGAAATCTGA+AGG 0.610305 8_3:-32506226 Msa1285950:intron
ATGGAAAGTGAGAAAAGAAG+AGG 0.612198 8_3:-32508758 Msa1285950:intron
GGGAAATCCAAGAAATGGAA+GGG 0.618012 8_3:-32508786 None:intergenic
GAACAACTTGATCAACAACA+TGG 0.662311 8_3:+32505808 None:intergenic
GGTCTTGTTCAACATAATGG+AGG 0.691225 8_3:-32507628 Msa1285950:CDS
TCGCCTTCCTAAGAATCACA+GGG 0.703131 8_3:-32506002 Msa1285950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTAATTTCTGAAAATTA+TGG - chr8_3:32506071-32506090 Msa1285950:CDS 10.0%
!! TATAATAAAAATTGACACAT+GGG + chr8_3:32507202-32507221 None:intergenic 15.0%
!! ATATAATAAAAATTGACACA+TGG + chr8_3:32507203-32507222 None:intergenic 15.0%
!!! AGTAACATTTTATTTTATAG+AGG + chr8_3:32507865-32507884 None:intergenic 15.0%
!!! GTACTTTTAAACTTAAATTT+AGG + chr8_3:32508370-32508389 None:intergenic 15.0%
!!! ATTCATTTGAAATATTTGCA+AGG - chr8_3:32506125-32506144 Msa1285950:intron 20.0%
!! ATCATCCAATAAAGTAAAAA+TGG - chr8_3:32506249-32506268 Msa1285950:CDS 20.0%
!! GAAAATATTCCAAAGAATTT+AGG - chr8_3:32507065-32507084 Msa1285950:intron 20.0%
!!! TAATATAATACCTCTTTTAC+TGG + chr8_3:32507106-32507125 None:intergenic 20.0%
!! ATAACCCAAATAAATATTAG+CGG + chr8_3:32507148-32507167 None:intergenic 20.0%
!!! ATATTTATTTGGGTTATGTT+TGG - chr8_3:32507151-32507170 Msa1285950:intron 20.0%
!!! TTTTTGTAACAAGGTTTATA+TGG - chr8_3:32508055-32508074 Msa1285950:intron 20.0%
!!! TTTTGTAACAAGGTTTATAT+GGG - chr8_3:32508056-32508075 Msa1285950:intron 20.0%
!! AATTAATCTAGAAACAGTTT+CGG + chr8_3:32508182-32508201 None:intergenic 20.0%
!! AAACTTAAATTTAGGTAAAG+AGG + chr8_3:32508362-32508381 None:intergenic 20.0%
!!! AGATAACTTTGCTTTATTTT+TGG - chr8_3:32508662-32508681 Msa1285950:intron 20.0%
!!! GATAACTTTGCTTTATTTTT+GGG - chr8_3:32508663-32508682 Msa1285950:intron 20.0%
!!! ATAACTTTGCTTTATTTTTG+GGG - chr8_3:32508664-32508683 Msa1285950:intron 20.0%
!!! CTTTGCTAGTTTTACTTTAT+TGG - chr8_3:32506012-32506031 Msa1285950:CDS 25.0%
!!! AATCCCTTTAAAGCTATTTT+AGG - chr8_3:32506045-32506064 Msa1285950:CDS 25.0%
!!! TGACCTAAAATAGCTTTAAA+GGG + chr8_3:32506051-32506070 None:intergenic 25.0%
!!! TTGACCTAAAATAGCTTTAA+AGG + chr8_3:32506052-32506071 None:intergenic 25.0%
!!! TGCAAACTAAGTTTTAATCT+TGG + chr8_3:32506180-32506199 None:intergenic 25.0%
!!! CTTTGCCATTTTTACTTTAT+TGG + chr8_3:32506257-32506276 None:intergenic 25.0%
! ATAAGTATGCCTAAATTCTT+TGG + chr8_3:32507077-32507096 None:intergenic 25.0%
! GTTTCCGCTAATATTTATTT+GGG - chr8_3:32507141-32507160 Msa1285950:intron 25.0%
!! GAAAAAATACTGTAACTTGT+CGG - chr8_3:32507263-32507282 Msa1285950:intron 25.0%
!! CAAAACCTAGAATTTAAGAT+CGG + chr8_3:32507730-32507749 None:intergenic 25.0%
!!! TACTGAGTTTTTTTGTAACA+AGG - chr8_3:32508046-32508065 Msa1285950:intron 25.0%
! CAAGAATCTTAATCAATTAC+AGG - chr8_3:32508227-32508246 Msa1285950:intron 25.0%
! TGAAATTCACATCAAAAGAA+AGG + chr8_3:32508609-32508628 None:intergenic 25.0%
! TCAAACTAAGAAAGTTGAAA+AGG - chr8_3:32508626-32508645 Msa1285950:intron 25.0%
! TTTTGTATATCTTGCCCTAA+CGG - chr8_3:32505889-32505908 Msa1285950:intron 30.0%
GAATGTAATCAATTCCGTTA+GGG + chr8_3:32505906-32505925 None:intergenic 30.0%
TGAATGTAATCAATTCCGTT+AGG + chr8_3:32505907-32505926 None:intergenic 30.0%
!! AACTTAGTTTGCATACTTTG+TGG - chr8_3:32506187-32506206 Msa1285950:intron 30.0%
!!! TTGCTCTGAGTTTTAAAACA+AGG + chr8_3:32506218-32506237 None:intergenic 30.0%
!!! TGTTTTAAAACTCAGAGCAA+AGG - chr8_3:32506218-32506237 Msa1285950:intron 30.0%
TGATATCAAATTGTACTGCA+AGG + chr8_3:32506337-32506356 None:intergenic 30.0%
! TTTTGTATATCTTGCGCTAA+CGG + chr8_3:32506380-32506399 None:intergenic 30.0%
! GAAAAGCTAGTTGATTCTAT+GGG + chr8_3:32506595-32506614 None:intergenic 30.0%
! TGAAAAGCTAGTTGATTCTA+TGG + chr8_3:32506596-32506615 None:intergenic 30.0%
AGAAGGTTTCAATAATGATG+TGG + chr8_3:32506653-32506672 None:intergenic 30.0%
AATTTCAAAAGTTCTGCAGA+AGG + chr8_3:32506670-32506689 None:intergenic 30.0%
ATTGAAGTCAGTTGAAAAAC+CGG - chr8_3:32506690-32506709 Msa1285950:intron 30.0%
!! TGAATGTTGATTTCGAACAT+CGG + chr8_3:32506789-32506808 None:intergenic 30.0%
TGTAAACCATTGTCAATAAC+TGG - chr8_3:32506877-32506896 Msa1285950:intron 30.0%
TTATTGTTCTCCAGTAAAAG+AGG - chr8_3:32507093-32507112 Msa1285950:intron 30.0%
CGTTTCCGCTAATATTTATT+TGG - chr8_3:32507140-32507159 Msa1285950:intron 30.0%
AATAAAAATTGACACATGGG+TGG + chr8_3:32507199-32507218 None:intergenic 30.0%
TTGAATTACAGTAGATCTGA+GGG + chr8_3:32507300-32507319 None:intergenic 30.0%
AGTTTCCGATCTTAAATTCT+AGG - chr8_3:32507722-32507741 Msa1285950:intron 30.0%
!! AGTTTGATGAACTGTTTCTA+CGG - chr8_3:32507908-32507927 Msa1285950:intron 30.0%
! GGTATATCATCTTTTGATGA+AGG + chr8_3:32508097-32508116 None:intergenic 30.0%
! ATGCATATATCCACATTTTC+TGG + chr8_3:32508496-32508515 None:intergenic 30.0%
CAAAAGAAAGGAAGACAAAA+AGG + chr8_3:32508597-32508616 None:intergenic 30.0%
! TGATCAAGTTGTTCAAGATT+TGG - chr8_3:32508740-32508759 Msa1285950:intron 30.0%
! TCTTTTCTCACTTTCCATGA+AGG + chr8_3:32505779-32505798 None:intergenic 35.0%
ATGGAAAGTGAGAAAAGAAG+AGG - chr8_3:32505781-32505800 Msa1285950:CDS 35.0%
TGATACCAAATTGTACTCCA+AGG - chr8_3:32505932-32505951 Msa1285950:intron 35.0%
!!! CTGAGTTTTAAAACAAGGCT+AGG + chr8_3:32506213-32506232 None:intergenic 35.0%
ATGGAAAGTGAGAAAAGAAG+AGG + chr8_3:32505781-32505800 None:intergenic 35.0%
AGGTTTCAATAATGATGTGG+AGG + chr8_3:32506650-32506669 None:intergenic 35.0%
CTCAGAAATAGGAAAGTTAC+CGG + chr8_3:32506712-32506731 None:intergenic 35.0%
!! ACTTTCCTATTTCTGAGTAG+TGG - chr8_3:32506715-32506734 Msa1285950:intron 35.0%
GTCAAAATGGTGACTTAGTT+AGG + chr8_3:32506744-32506763 None:intergenic 35.0%
ATCAACATTCACTTCATCGA+TGG - chr8_3:32506798-32506817 Msa1285950:intron 35.0%
TCAACATTCACTTCATCGAT+GGG - chr8_3:32506799-32506818 Msa1285950:intron 35.0%
TTTAACGTTAGGTTCCCATT+TGG + chr8_3:32506829-32506848 None:intergenic 35.0%
GTGTTGCAAACTTTAACGTT+AGG + chr8_3:32506840-32506859 None:intergenic 35.0%
TCTGAACCAGTTATTGACAA+TGG + chr8_3:32506886-32506905 None:intergenic 35.0%
! TCAATAACTGGTTCAGAAAG+AGG - chr8_3:32506889-32506908 Msa1285950:intron 35.0%
! CAATAACTGGTTCAGAAAGA+GGG - chr8_3:32506890-32506909 Msa1285950:intron 35.0%
AGCTTTCCTTTGATCTCAAA+AGG + chr8_3:32506938-32506957 None:intergenic 35.0%
GTGATTAGAGGAACTTGATT+TGG + chr8_3:32506965-32506984 None:intergenic 35.0%
GATGTATCTTCTGTGATTAG+AGG + chr8_3:32506977-32506996 None:intergenic 35.0%
GTTGAATTACAGTAGATCTG+AGG + chr8_3:32507301-32507320 None:intergenic 35.0%
GTGTAAGAGACATGTATAGT+TGG + chr8_3:32507429-32507448 None:intergenic 35.0%
!!! TTTCTTTTGGTGCTTTAGGT+TGG - chr8_3:32507664-32507683 Msa1285950:intron 35.0%
!! GTTTGATGAACTGTTTCTAC+GGG - chr8_3:32507909-32507928 Msa1285950:intron 35.0%
AAAACTATCCTGCCACATAA+GGG - chr8_3:32508117-32508136 Msa1285950:intron 35.0%
GAAGCAGTATTTATACCTGT+TGG - chr8_3:32508284-32508303 Msa1285950:intron 35.0%
CTTTGTATCACCAGAAAATG+TGG - chr8_3:32508483-32508502 Msa1285950:intron 35.0%
GAACAACTTGATCAACAACA+TGG + chr8_3:32508734-32508753 None:intergenic 35.0%
TTTCATTAGATGCTCATCAC+TGG - chr8_3:32508767-32508786 Msa1285950:CDS 35.0%
TGACTCCTTGGAGTACAATT+TGG + chr8_3:32505940-32505959 None:intergenic 40.0%
AATTGTACTCCAAGGAGTCA+TGG - chr8_3:32505940-32505959 Msa1285950:intron 40.0%
AGATGTTAACCATGACTCCT+TGG + chr8_3:32506317-32506336 None:intergenic 40.0%
TTACCGCTGTAACTTTCATG+CGG - chr8_3:32505972-32505991 Msa1285950:intron 40.0%
TTACCGCTGTAACTTTCATG+CGG + chr8_3:32505972-32505991 None:intergenic 40.0%
AATTGTACTGCAAGGAGTCA+TGG + chr8_3:32506329-32506348 None:intergenic 40.0%
! TCTTTTCTCACTTTCCATGC+AGG - chr8_3:32506490-32506509 Msa1285950:intron 40.0%
GGGAAATCCAACAAATGGAA+GGG + chr8_3:32506516-32506535 None:intergenic 40.0%
AGGGAAATCCAACAAATGGA+AGG + chr8_3:32506517-32506536 None:intergenic 40.0%
! TTCCATTTGTTGGATTTCCC+TGG - chr8_3:32506516-32506535 Msa1285950:intron 40.0%
ACAAGCGGATGATAGAAGAT+AGG + chr8_3:32506572-32506591 None:intergenic 40.0%
! AGTTGATTCTATGGGACAAG+CGG + chr8_3:32506587-32506606 None:intergenic 40.0%
!! GAGGGTCTTGTTCAACATAA+TGG - chr8_3:32506908-32506927 Msa1285950:intron 40.0%
!! GGTCTTGTTCAACATAATGG+AGG - chr8_3:32506911-32506930 Msa1285950:intron 40.0%
GAAGATACATCAGAAACAGC+TGG - chr8_3:32506986-32507005 Msa1285950:intron 40.0%
ATGGGTGGTATGGCAATTAA+AGG + chr8_3:32507184-32507203 None:intergenic 40.0%
AAATTGACACATGGGTGGTA+TGG + chr8_3:32507194-32507213 None:intergenic 40.0%
TAGATCTGAGGGCAATGAAA+GGG + chr8_3:32507289-32507308 None:intergenic 40.0%
CATGTATAGTTGGAGAGGAA+GGG + chr8_3:32507419-32507438 None:intergenic 40.0%
ACATGTATAGTTGGAGAGGA+AGG + chr8_3:32507420-32507439 None:intergenic 40.0%
AGAGACATGTATAGTTGGAG+AGG + chr8_3:32507424-32507443 None:intergenic 40.0%
ATCGTTGAAAGAGGAAAGAG+TGG + chr8_3:32507544-32507563 None:intergenic 40.0%
TTAACGAGTTGTTCTGCGAT+AGG + chr8_3:32507570-32507589 None:intergenic 40.0%
!! GTTTCAGGTCGTGTTTCTTT+TGG - chr8_3:32507651-32507670 Msa1285950:intron 40.0%
!!! CGTGTTTCTTTTGGTGCTTT+AGG - chr8_3:32507660-32507679 Msa1285950:intron 40.0%
GGTTCTTCTGAATTGTGAGA+AGG - chr8_3:32507930-32507949 Msa1285950:intron 40.0%
TCTTCTGAATTGTGAGAAGG+AGG - chr8_3:32507933-32507952 Msa1285950:intron 40.0%
! CTTATGTGGCAGGATAGTTT+TGG + chr8_3:32508118-32508137 None:intergenic 40.0%
CAAAACTATCCTGCCACATA+AGG - chr8_3:32508116-32508135 Msa1285950:intron 40.0%
AAACTATCCTGCCACATAAG+GGG - chr8_3:32508118-32508137 Msa1285950:intron 40.0%
! AAGAGTGTGTTTCCAGCTAT+GGG - chr8_3:32508146-32508165 Msa1285950:intron 40.0%
CAATTACAGGTGTAGTACCT+TGG - chr8_3:32508240-32508259 Msa1285950:intron 40.0%
! TCAGATTTCGAACTTGGTGA+GGG + chr8_3:32508313-32508332 None:intergenic 40.0%
! TTCAGATTTCGAACTTGGTG+AGG + chr8_3:32508314-32508333 None:intergenic 40.0%
TCACCAAGTTCGAAATCTGA+AGG - chr8_3:32508313-32508332 Msa1285950:intron 40.0%
TCACCTTCAGATTTCGAACT+TGG + chr8_3:32508319-32508338 None:intergenic 40.0%
GGCAAGAGAAGACAAACTTA+AGG - chr8_3:32508558-32508577 Msa1285950:intron 40.0%
ATGTATGCCAGCAGATGCAA+TGG + chr8_3:32506548-32506567 None:intergenic 45.0%
GGAAGCCACTACTCAGAAAT+AGG + chr8_3:32506723-32506742 None:intergenic 45.0%
!! TAAGTCACCATTTTGACCCC+AGG - chr8_3:32506747-32506766 Msa1285950:intron 45.0%
!! ACCATTTTGACCCCAGGAAT+CGG - chr8_3:32506753-32506772 Msa1285950:intron 45.0%
!!! GGAGGTCCTTTTGAGATCAA+AGG - chr8_3:32506929-32506948 Msa1285950:intron 45.0%
AGAAACAGCTGGTGCACTTT+GGG - chr8_3:32506997-32507016 Msa1285950:intron 45.0%
GTAGATCTGAGGGCAATGAA+AGG + chr8_3:32507290-32507309 None:intergenic 45.0%
TGAGGTTGGAGAAACAAACG+AGG + chr8_3:32507389-32507408 None:intergenic 45.0%
GGAGTGAAGGAATTGAGGAA+TGG + chr8_3:32507478-32507497 None:intergenic 45.0%
ACGATGGAGTGAAGGAATTG+AGG + chr8_3:32507483-32507502 None:intergenic 45.0%
GAAAAGTGACGATGGAGTGA+AGG + chr8_3:32507491-32507510 None:intergenic 45.0%
GAGGTGAAACAAGGAACTGA+GGG + chr8_3:32507515-32507534 None:intergenic 45.0%
AGAGGTGAAACAAGGAACTG+AGG + chr8_3:32507516-32507535 None:intergenic 45.0%
GATAGGTGCATCGTTGAAAG+AGG + chr8_3:32507553-32507572 None:intergenic 45.0%
ACAAATTCGCGGCTAACTGA+GGG + chr8_3:32507624-32507643 None:intergenic 45.0%
AAACCCTAGCGACAAATTCG+CGG + chr8_3:32507635-32507654 None:intergenic 45.0%
GAATTTGTCGCTAGGGTTTC+AGG - chr8_3:32507636-32507655 Msa1285950:CDS 45.0%
! CAAGAGTGTGTTTCCAGCTA+TGG - chr8_3:32508145-32508164 Msa1285950:intron 45.0%
AGCTTCTGCTCAAAAGTCCA+AGG + chr8_3:32508260-32508279 None:intergenic 45.0%
! TGGACTTTTGAGCAGAAGCT+TGG - chr8_3:32508260-32508279 Msa1285950:intron 45.0%
TCGCCTTCCTAAGAATCACA+GGG - chr8_3:32508537-32508556 Msa1285950:intron 45.0%
TTGCCCTGTGATTCTTAGGA+AGG + chr8_3:32508543-32508562 None:intergenic 45.0%
TCTCTTGCCCTGTGATTCTT+AGG + chr8_3:32508547-32508566 None:intergenic 45.0%
ACGCCGCATGAAAGTTACAG+CGG + chr8_3:32506291-32506310 None:intergenic 50.0%
ACGCCGCATGAAAGTTACAG+CGG - chr8_3:32506291-32506310 Msa1285950:CDS 50.0%
AACAAATGGAAGGGCCTGCA+TGG + chr8_3:32506507-32506526 None:intergenic 50.0%
ATGCAGGCCCTTCCATTTGT+TGG - chr8_3:32506506-32506525 Msa1285950:intron 50.0%
CGCCAGGGAAATCCAACAAA+TGG + chr8_3:32506521-32506540 None:intergenic 50.0%
AGATGCAATGGTAGACGCCA+GGG + chr8_3:32506536-32506555 None:intergenic 50.0%
! ACCGATTCCTGGGGTCAAAA+TGG + chr8_3:32506757-32506776 None:intergenic 50.0%
TGACCCCAGGAATCGGTATT+TGG - chr8_3:32506760-32506779 Msa1285950:intron 50.0%
GACCCCAGGAATCGGTATTT+GGG - chr8_3:32506761-32506780 Msa1285950:intron 50.0%
ACCCCAGGAATCGGTATTTG+GGG - chr8_3:32506762-32506781 Msa1285950:intron 50.0%
!! TCATCGATGGGAGCACCAAA+TGG - chr8_3:32506811-32506830 Msa1285950:intron 50.0%
!! CATCGATGGGAGCACCAAAT+GGG - chr8_3:32506812-32506831 Msa1285950:intron 50.0%
CAGAAACAGCTGGTGCACTT+TGG - chr8_3:32506996-32507015 Msa1285950:intron 50.0%
!! CCTTGGTTTGTACGACCGAA+GGG + chr8_3:32507354-32507373 None:intergenic 50.0%
GGAAGGGTTAGAGATGAGGT+TGG + chr8_3:32507403-32507422 None:intergenic 50.0%
GAGAGGAAGGGTTAGAGATG+AGG + chr8_3:32507407-32507426 None:intergenic 50.0%
CTGAGGGAGAAAAGTGACGA+TGG + chr8_3:32507499-32507518 None:intergenic 50.0%
TGGCGATGAGAGGTGAAACA+AGG + chr8_3:32507524-32507543 None:intergenic 50.0%
GACAAATTCGCGGCTAACTG+AGG + chr8_3:32507625-32507644 None:intergenic 50.0%
TTAGCCGCGAATTTGTCGCT+AGG - chr8_3:32507628-32507647 Msa1285950:CDS 50.0%
TAGCCGCGAATTTGTCGCTA+GGG - chr8_3:32507629-32507648 Msa1285950:CDS 50.0%
GTGAGAAGGAGGATGAGAAG+AGG - chr8_3:32507944-32507963 Msa1285950:intron 50.0%
AGGATGAGAAGAGGAGTGAG+CGG - chr8_3:32507953-32507972 Msa1285950:intron 50.0%
ACTCTTGCACACCCCTTATG+TGG + chr8_3:32508132-32508151 None:intergenic 50.0%
GGTAAGTGAACACCCATAGC+TGG + chr8_3:32508161-32508180 None:intergenic 50.0%
GTCGCCTTCCTAAGAATCAC+AGG - chr8_3:32508536-32508555 Msa1285950:intron 50.0%
CAGATGCAATGGTAGACGCC+AGG + chr8_3:32506537-32506556 None:intergenic 55.0%
GCGTCTACCATTGCATCTGC+TGG - chr8_3:32506538-32506557 Msa1285950:intron 55.0%
!! CCCCCAAATACCGATTCCTG+GGG + chr8_3:32506766-32506785 None:intergenic 55.0%
CCCCAGGAATCGGTATTTGG+GGG - chr8_3:32506763-32506782 Msa1285950:intron 55.0%
!! GCCCCCAAATACCGATTCCT+GGG + chr8_3:32506767-32506786 None:intergenic 55.0%
GGTGTCAGCAGCAACGTTTG+TGG + chr8_3:32507323-32507342 None:intergenic 55.0%
GGTTTGTACGACCGAAGGGA+TGG + chr8_3:32507350-32507369 None:intergenic 55.0%
CCCTTCGGTCGTACAAACCA+AGG - chr8_3:32507351-32507370 Msa1285950:intron 55.0%
!! GCCTTGGTTTGTACGACCGA+AGG + chr8_3:32507355-32507374 None:intergenic 55.0%
GAGGAAAGAGTGGCGATGAG+AGG + chr8_3:32507534-32507553 None:intergenic 55.0%
CGTAGGAAGCAGAGAGGTTG+AGG + chr8_3:32507599-32507618 None:intergenic 55.0%
AGGGATCGTAGGAAGCAGAG+AGG + chr8_3:32507605-32507624 None:intergenic 55.0%
ATGAGAAGAGGAGTGAGCGG+CGG - chr8_3:32507956-32507975 Msa1285950:intron 55.0%
TTGCACACCCCTTATGTGGC+AGG + chr8_3:32508128-32508147 None:intergenic 55.0%
!! ACTTGGTGAGGGCATCCAAC+AGG + chr8_3:32508302-32508321 None:intergenic 55.0%
!! GGCCCCCAAATACCGATTCC+TGG + chr8_3:32506768-32506787 None:intergenic 60.0%
TGACACCGCTGCCATCCCTT+CGG - chr8_3:32507336-32507355 Msa1285950:intron 60.0%
TACGACCGAAGGGATGGCAG+CGG + chr8_3:32507344-32507363 None:intergenic 60.0%
CGAGGCAGATTGTGCTGCCT+TGG + chr8_3:32507371-32507390 None:intergenic 60.0%
GCGGCTAACTGAGGGATCGT+AGG + chr8_3:32507616-32507635 None:intergenic 60.0%
! ACGGTGACGGTGAAGGAGAG+AGG - chr8_3:32507979-32507998 Msa1285950:intron 60.0%
! CGGTGACGGTGAAGGAGAGA+GGG - chr8_3:32507980-32507999 Msa1285950:intron 60.0%
! GGTGACGGTGAAGGAGAGAG+GGG - chr8_3:32507981-32508000 Msa1285950:intron 60.0%
GTGACGGTGAAGGAGAGAGG+GGG - chr8_3:32507982-32508001 Msa1285950:intron 60.0%
AAGGAGAGAGGGGGCGATGA+TGG - chr8_3:32507991-32508010 Msa1285950:intron 60.0%
GAAGAGGAGTGAGCGGCGGA+CGG - chr8_3:32507960-32507979 Msa1285950:intron 65.0%
! GAGAGAGGGGGCGATGATGG+TGG - chr8_3:32507994-32508013 Msa1285950:intron 65.0%
!! AGAGGGGGCGATGATGGTGG+AGG - chr8_3:32507997-32508016 Msa1285950:intron 65.0%
GAGTGAGCGGCGGACGGTGA+CGG - chr8_3:32507966-32507985 Msa1285950:intron 70.0%
! GCGGCGGACGGTGACGGTGA+AGG - chr8_3:32507972-32507991 Msa1285950:intron 75.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 32505767 32508794 32505767 ID=Msa1285950;Name=Msa1285950
chr8_3 mRNA 32505767 32508794 32505767 ID=Msa1285950-mRNA-1;Parent=Msa1285950;Name=Msa1285950-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|0.5|0.6|5|0|141
chr8_3 exon 32508759 32508794 32508759 ID=Msa1285950-mRNA-1:exon:10051;Parent=Msa1285950-mRNA-1
chr8_3 exon 32507554 32507656 32507554 ID=Msa1285950-mRNA-1:exon:10050;Parent=Msa1285950-mRNA-1
chr8_3 exon 32506227 32506312 32506227 ID=Msa1285950-mRNA-1:exon:10049;Parent=Msa1285950-mRNA-1
chr8_3 exon 32505982 32506101 32505982 ID=Msa1285950-mRNA-1:exon:10048;Parent=Msa1285950-mRNA-1
chr8_3 exon 32505767 32505847 32505767 ID=Msa1285950-mRNA-1:exon:10047;Parent=Msa1285950-mRNA-1
chr8_3 CDS 32508759 32508794 32508759 ID=Msa1285950-mRNA-1:cds;Parent=Msa1285950-mRNA-1
chr8_3 CDS 32507554 32507656 32507554 ID=Msa1285950-mRNA-1:cds;Parent=Msa1285950-mRNA-1
chr8_3 CDS 32506227 32506312 32506227 ID=Msa1285950-mRNA-1:cds;Parent=Msa1285950-mRNA-1
chr8_3 CDS 32505982 32506101 32505982 ID=Msa1285950-mRNA-1:cds;Parent=Msa1285950-mRNA-1
chr8_3 CDS 32505767 32505847 32505767 ID=Msa1285950-mRNA-1:cds;Parent=Msa1285950-mRNA-1
Gene Sequence

>Msa1285950

ATGGAAGGGCCTTCATGGAAAGTGAGAAAAGAAGAGAAAGAGGGTCTTGTTCAACATAATGGAGGTCCTTTTGAGATCAAAGGAAAGCTATTTCCAAATCAAGTTCCTCTAATCACAGAAGATACATCAGAAACAGCTGGTGTAGTACCTTGGACTTTTGAGCAGAAGCTTGGTGAAGCAGTATTTATACCTGTTGGATGCCCTCACCAAGTTCGAAATCTGAAGTCATGCACAAAAGTTGCAGTAGACTTTGTATCACCAGAAAATGTGGATATATGCATGCTTTTAAGTGAAGAGTTTCGTCGCCTTCCTAAGAATCACAGGGCAAGAGAAGACAAACTTAAGATAAAAAAGATGATAATCCATGTTGTTGATCAAGTTGTTCAAGATTTGGAAGCTTTCATTAGATGCTCATCACTGGACTAA
Protein sequence

>Msa1285950

MEGPSWKVRKEEKEGLVQHNGGPFEIKGKLFPNQVPLITEDTSETAGVVPWTFEQKLGEAVFIPVGCPHQVRNLKSCTKVAVDFVSPENVDICMLLSEEFRRLPKNHRAREDKLKIKKMIIHVVDQVVQDLEAFIRCSSLD*